Khaled Osman - Academia.edu (original) (raw)

Papers by Khaled Osman

African Journal of Food Science, Apr 30, 2010

Environmental Toxicology and Pharmacology, 2019

Asian Journal of Chemistry, 2015

The median lethal dose (LD50) and the effect of the sublethal dose of 3.014 mg/kg as single or re... more The median lethal dose (LD50) and the effect of the sublethal dose of 3.014 mg/kg as single or repetitive doses every other day for 28 days of imidacloprid (IMI) on the domestic Japanese quail as a bioindicator for xenobiotic detection were investigated. The LD50 was found to be 75.35 mg/kg indicating that IMI is highly toxic to Japanese quail. The sub-acute study revealed that certain biomarkers in the selected tissues of the quail such as acetylcholinesterase (AChE), butyrylcholinesterase (BuChE), aminotransaminases (alanine aminotransferase, ALT and aspartate aminotransaminase, AST), phosphatases (acid phosphatase, ACP and alkaline phosphatase, ALP), lactate dehydrogenase (LDH), adenosine -triphosphatase (ATP-ase), glutathione-S-transferase (GST), lipid peroxidation (LPO) and blood glucose showed significant inductions, while significant reductions in the levels of glutathione reduced (GSH), DNA and RNA were recorded. It can be concluded that IMI can induce neurotoxicity, hepatot...

Journal of food science and technology, 2015

Distilled water and ozonated water as postharvest wash treatments for 15-60 min as dipping times ... more Distilled water and ozonated water as postharvest wash treatments for 15-60 min as dipping times were tested to remove two acaricides namely, dicofol and amitraz from different varieties of date fruits. Recovered amount of the acaricides was extracted using solid phase extraction (SPE) and then analyzed by gas chromatography-mass spectrometry (GC-MS). Dicofol removal percentages in the presence of ozone increased in the order of Nabtet Aly > Nabout Seif > Khalas > Sakay, while amitraz removal increased in the order of Nabtet Aly > Nabout Seif > Sakay > Khalas, respectively, and the percentages of residues on date fruits depended on the dipping time. Kinetic studies revealed that dicofol and amitraz were easily removable from date fruits treated with ozonated water. Ozone-treated date palm fruits were not associated with significant changes in antioxidant capacity, and phenolic and sugar contents. Due to the large amount of dates consumed by Saudi residents, a highe...

International Journal of Phytoremediation, 2015

The research was designated to study the ability of plants to bio-accumulate, translocate and rem... more The research was designated to study the ability of plants to bio-accumulate, translocate and remove the heavy metals, lead and cadmium from contaminated soil. The herbal plant ryegrass, Lolium multiflorum was investigated as a bio-accumulator plant for these metals. The translocation of these heavy metals in the herbal plant was compared considering root to shoot transport and redistribution of metals in the root and shoot system. The trace metal contents from root and shoot parts were determined using atomic absorption spectrometer. The results showed that the percent of lead and cadmium transferred to ryegrass plant were averaged as 51.39, and 74.57%, respectively, while those remained in the soil were averaged as 48.61 and 25.43% following 60 days of treatment. The soil-plant transfer index in root and shoot system of ryegrass was found to be 0.32 and 0.20 for lead, and 0.50 and 0.25 for cadmium. These findings indicated that the herbal plant ryegrass, Lolium multiflorum is a good accumulator for cadmium than lead. The soil-plant transfer factor (the conc. of heavy metal in plant to the conc. in soil) indicated that the mechanism of soil remedy using the investigated plant is phytoextraction where the amounts of heavy metals transferred by plant roots into the above ground portions were higher than that remained in the soil. The method offers green technology solution for the contamination problem since it is effective technology with minimal impact on the environment and can be easily used for soil remedy.

The present study is dealing with the development of a phytoremediation system such as using the ... more The present study is dealing with the development of a phytoremediation system such as using the herbal plant ryegrass, Lolium multiflorum as excluder plant to investigate its ability to exclude the two metals, copper and zinc from contaminated soil. The plant was grown in different levels of metals-contaminated soil. The translocation of metals in the plant was compared considering root to shoot transport and redistribution of metals in the root and shoot system. The trace metal contents from root and shoot parts were determined using atomic absorption spectrometer. The results showed that the percent of copper transferred to ryegrass plant was 96.01%, while the remained percentage in soil was 3.99% following 60 days of treatment. On the other hand, the percent of zinc transferred to ryegrass plant was 84.51%, while the remained percentage in soil was 15.49% following the same time interval. The soil-plant transfer index in root and shoot system of ryegrass was found to be 0.60 and...

Journal of Food Science and Technology, 2012

The effectiveness of different postharvest wash treatments at two levels (10 and 20 g/l) for diff... more The effectiveness of different postharvest wash treatments at two levels (10 and 20 g/l) for different dipping times was determined to remove chlorpyrifos from date fruits treated at concentration of 2 mg/l. The recovered amount of chlorpyrifos was extracted based on the solid phase extraction (SPE) method and then analyzed by gas chromatography with mass spectrometry (GC-MS). The results demonstrate that the removal of chlorpyrifos increased in the order of acetic acid (AA)> citric acid (CA)> hydrogen peroxide (H 2 O 2)> potassium permanganate (KMnO 4)> running water (H 2 O), and the percent of pesticide residue on date fruits depended on the concentration of tested washing treatments and dipping time without the formation of the toxic by-product, chlorpyrifos-oxon. Kinetic studies revealed that chlorpyrifos was found to be more easily removable from date fruits treated with the tested chemical solutions with t 1/2 values of 12-29 min compared with roughly 53 min in case of running water. The impact of these washing treatments on quality of date fruits illustrated that all treatments exerted a little negative effect on total sugars content but H 2 O 2 and KMnO4 at level of 2 % had more drastic effect. Whereas, running water, 10 and 20 g/l CA caused significant increases in total phenolic contents, during all the tested contact times compared with control. Except the insignificant effect of KMnO 4 treatments, antioxidant capacity of date fruits tended to increase in all wash treatments, when the contact times were 5 or 15 min.

Journal of Environmental Science and Health, Part B, 1992

The bran toxic baits (0.5 % w/w) of five oxime carbamate pesticides; aldicarb, aldoxycarb, methom... more The bran toxic baits (0.5 % w/w) of five oxime carbamate pesticides; aldicarb, aldoxycarb, methomyl, oxamyl and thiofanox were tested for their molluscicidal activity against Theba pisana snails under Laboratory conditions. In addition, the in vivo effects of these compounds on seven vital enzymes namely Acetylcholin‐esterase (AchE), glutathion‐S‐transferase (GST), glutamic oxlaoacetic transaminase (GOT), glutamic pyruvic transaminase (GPT), acid phosphatase (AcP),

International Biodeterioration & Biodegradation, 2009

The bioremediation of the nematicide oxamyl, applied at the recommended rate of 6lha−1 in sandy s... more The bioremediation of the nematicide oxamyl, applied at the recommended rate of 6lha−1 in sandy soil cultivated with tomato and amended with different animal manures at the recommended dose of 2.5tonsha−1, was investigated. The experiment was conducted in a controlled environmental chamber under a 16-h photoperiod, with a light intensity of 300μEm−2s−1 at 25°C and relative humidity of 70±5%. The

Food Control, 2011

Residues of 23 pesticides (13 insecticides, 3 fungicides, 3 herbicides, 2 acaricides, 1 rodentici... more Residues of 23 pesticides (13 insecticides, 3 fungicides, 3 herbicides, 2 acaricides, 1 rodenticide and 1 nematicide) in 160 different vegetable samples cultivated under greenhouse conditions and collected from 4 major supermarkets located in Al-Qassim region, Saudi Arabia were monitored. A multiresidue method was carried out by gas chromatography with mass spectrometry (GCeMS). Residues were found in 89 samples. Fifty four samples were found above the maximum residue levels (MRLs). The most frequently found pesticides were carbaryl followed by biphenyl and then carbofuran. MRL values exceeded most often in cabbage (11 samples) followed by squash, green pepper and carrot (7 samples each) followed by cucumber and lettuce (6 samples each), eggplant (5 samples) and then tomato (4 samples). Also, cabbage showed the highest contamination rates (16 samples), followed by carrot, cucumber and green pepper (12 samples each), squash (11 samples), lettuce and tomato (9 samples each) and eggplant (8 samples). The highest concentrations were determined in lettuce followed by squash, cabbage and carrot. Al-Qassim region population's average daily intake (EDI) has been estimated between 2 Â 10 À5 and 0.597 mg/kg body weight/day and the hazard index (EDI/acceptable daily intake (ADI)) less than the unity for the tested compounds. The data illustrated that the intakes are much lower than the ADIs and the exposure level to whole pesticides was below the level to produce health risk. The data indicated also the need for regular monitoring programs for pesticide residues in vegetables cultivated under greenhouse conditions, at the national level, to protect consumers' health.

Journal of Food Science and Technology

This study aimed to determine the levels of epichlorohydrin (ECH) and some metals in 3 brands of ... more This study aimed to determine the levels of epichlorohydrin (ECH) and some metals in 3 brands of bagged and loose black teas which are widely marketed in Alexandria markets, Egypt, and estimate the acceptable daily intake as a safety indicator. Gas chromatography–mass spectrometry results revealed that ECH levels significantly differed between the tested brands steeped for 2 min and tea bags contained higher levels than in loose teas and the levels increased by increasing the steep time. These levels of ECH in all the tested brands either in tea bags or loose teas were higher than the guideline value of 0.10 μg/L. Also, the results illustrated that the adding of sucrose or washing of bags with deionized water for 1 min significantly decreased the levels of ECH. In addition, inductively coupled plasma optic emission spectrometry (ICP–OES) results illustrated that the levels of Al, Pb, and Cd were too low in infusions compared to the established guidelines. Because of the high consump...

Nonpoint source (NPS) pollution from agriculture is the leading source of impairment to Survey Ri... more Nonpoint source (NPS) pollution from agriculture is the leading source of impairment to Survey Rivers and lakes. Pesticides are one of the major NPS pollutants that result from agricultural activities. Among those pesticides, is Bromoxynil (BRMX) which is a widely used herbicide. The present study was carried out to determine the capability of selected biological control agents to degrade BRMX at different incubation periods. Microbial degradation of BRMX at the rate of 100 ppm in pure liquid culture media of either seven isolates of bacteria; Azotobacter chroococcum, Azospirillium barasilense, Klebsilense pneumoneae, Pseudomonas cepacia, Pseudomonas fluoresences, Bacillus subtilis, and Bacillus polymyxa or two isolates of fungi; Trichoderma viride and Trichoderma harzianum has been determined at different time intervals using high performance liquid chromatography (HPLC) with UV detection, and solid phase extraction (SPE) technique. A biphasic model was assumed in order to carry ou...

Alexandria Science Exchange Journal, 2019

The ameliorating effect of 150 mg/kg b.w. of Nacetylcysteine (NAC) against the oral administratio... more The ameliorating effect of 150 mg/kg b.w. of Nacetylcysteine (NAC) against the oral administration of 7.88 (1/25 LD50) or 202.07 (1/10 LD50) mg/kg/day for 14 days of chlorpyrifos-ethyl (CPF-E) and chlorpyrifosmethyl (CPF-M), respectively, was investigated using neurobehavioral and biochemical markers for this toxicity. Neurobehavioral tests; open field test (OFT), hole-board test (HBT), light/dark box test (LDBT) and elevated plus maze (EPM) showed increase frequency of exploration, low level of anxiety and locomotor in rats treated with either CPE-E or CPF-M, while the coadministration of NAC to treated rats attenuated neurobehavioral parameters. Biomarkers such as acetylcholinesterase (AChE), butyrylcholinesterase (BuChE), paraoxonase (PON) and adenosine 5'triphosphatase, (ATP-ase) showed declining in their activities, while calcium (Ca +2) levels in brain were increased. However, the administration of NAC following the intoxification of CPF-E or CPF-M attenuated the values of these biomarkers. It can be concluded that NAC can be used to ameliorate the toxicity of certain organophosphorus compounds such as CPF-E and CPF-M and considered it as a choice for the prevention and treatment of either CPF-E or CPF-M-induced toxicity.

International Journal of Food and Nutritional Science, 2017

The removal of chlorpyrifos pesticide residues from vegetables was achieved by using low level of... more The removal of chlorpyrifos pesticide residues from vegetables was achieved by using low level of ozonated water (OZW) for 15-60 min as contact times at 25 and 35ºC. Recovered amount of chlorpyrifos was extracted using solid phase extraction (SPE) and then analyzed by gas chromatography-mass spectrometry (GC-MS). The initial levels of residual chlorpyrifos varied with the kind of vegetables, where arugula had the highest level followed by parsley, leek, tomato, carrot, cucumber, cabbage, and then bell pepper. When vegetables washed in OZW was at 25 ºC, the percentages of chlorpyrifos removal were time-dependent and ranged from 30-83, 91-97, 80-92, 92-95, 87-97, 95-97, 64-100 and 90-97% for bell pepper, tomato, cucumber, carrot, arugula, parsley, cabbage and leek, respectively. In case of vegetables washed with OZW at 35ºC, increasing of the contact time was not significantly effect on the removal percentages of chlorpyrifos except with arugula and cabbage. Likewise, the increasing of OZW temperature caused a negative consequence on the removal percentages of pesticide. The effect of these wash treatments on vegetable quality parameters indicated that the removing of chlorpyrifos by using OZW did not produce any significant undesirable effects on antioxidant capacity, total phenolic contents and vitamin C of the tested vegetables. Due to the large amount of vegetables consumed in fresh form, a higher risk of exposure to chlorpyrifos may occur and the search for a safety method to remove this pesticide with negligible residual deposits has always been preferred. Therefore, the present study validated that ozone technology as wash treatment is safe and promising processes for the removal of chlorpyrifos from the vegetable's surface under domestic conditions to reduce the impact over consumer's health.

Journal of Agricultural and Marine Sciences [JAMS], 2005

The fate of pre-harvest-sprayed dicofol (DCF) on date fruits has been investigated. Date palm tre... more The fate of pre-harvest-sprayed dicofol (DCF) on date fruits has been investigated. Date palm trees, variety Sukkari, were sprayed with DCF (18.5%, EC) at the rate of 200 ml/100 L. DCF residues in date fruits were determined at different time intervals using high performance liquid chromatography (HPLC) with UV detection at 220 nm. The method was based on extraction with ethyl acetate. The results showed that the HPLC response was linear (r2 > 0.98) for DCF in the range of 0.0 to 2 mμg. The limits of detection and quantification were 0.24 and 0.80 ppm, respectively. The method was developed by using spiked date fruits at levels of 0.25, 0.50 and 1.0 ppm. Recovery percentage was satisfactory with a range of 77.2 to 103.6% and an RSD ranging from 6.33 to 11.84%. A biphasic model was assumed in order to carry out the statistical study of the loss of DCF from date fruits. The results showed that the initial deposit of DCF on date fruits was 11.22 ppm, while the residue value decrease...

The currently used techniques for soil remediation such as landfilling or metal extraction using ... more The currently used techniques for soil remediation such as landfilling or metal extraction using toxic agents as well as the use of metal-chelating bio-surfactant need to be replaced by green technology solution. Therefore, the research was designated to study the ability of plants to bio-accumulate, translocate and remove some metals such as copper, zinc, lead and cadmium from contaminated soil. The herbal plant ryegrass, Lolium multiflorum was investigated as a bio-accumulator plant for these metals. The translocation of these heavy metals in ryegrass was compared considering root to shoot transport and redistribution of metals in the root and shoot system. The trace metal contents from root and shoot parts were determined using atomic absorption spectrometer. The results showed that the percent of copper, zinc, lead and cadmium transferred to ryegrass plant were 96.01, 84.51, 51.39, and 74.57%, respectively, while those remained in the soil were 3.81, 14.42, 45.16 and 27.40 % fol...