Khaled Sharafeldin - Academia.edu (original) (raw)

Papers by Khaled Sharafeldin

The effect of acute and chronic exposure of pesticide profenofos on its accumulation and some hem... more The effect of acute and chronic exposure of pesticide profenofos on its accumulation and some hematological parameters of the Nile tilapia (Oreochromis niloticus) were studied. The 96 hrs LC 50 of profenofos to Nile tilapia was determined to be 0.87 mg/l. No detectable profenofos in aquaria water was observed after 14, 21 and 28 days of trial period and during recovery. Profenofos were accumulated in higher rate fish tissues during the acute exposure. However, during chronic period a gradual increase was observed through 1 to 7 days followed by gradual decrease until it was not detectable at the end of 28 days. Regarding hematology, highly significant increase in WBCs counts during both the acute and chronic exposure to was observed. Highly significant decrease in RBCs counts, Hb content and Hct % was noticed during experimental periods. For most of the recorded data, it showed marked improvement during the recovery period. There was a significant increase in MCHC during both acute ...

Aquaculture, 2015

The present study was undertaken to evaluate the use of roasted coffee powder (RCP; Coffea arabic... more The present study was undertaken to evaluate the use of roasted coffee powder (RCP; Coffea arabica) in practical diets for common carp, Cyprinus carpio L. to improve their growth, biochemical status, and resistance against Zn toxicity. However, RCP was added to the ingredients of tested diets to represent 0.0 (control), 0.50, 1.0, 2.0, or 5.0 g/kg diet. Fish (10.2 ± 0.42 g) were distributed into various treatments at a rate of 20 fish per 100-L aquarium and fed one of the experimental diets for 10 weeks in triplicates. After the feeding trial, fish from each treatment were further-exposed to 5.0 mg Zn/L for 7 days. It is noticed that final fish performance was not significantly (P b 0.05) affected by increasing RCP levels up to 1.0 g/kg after which fish growth declined. Moreover, fish fed diets containing 2.0-5.0 g RCP/kg consumed less diet than the other treatments giving highest FCRs (1.46 and 1.53, respectively), whereas fish fed 0.0-1.0 RCP/kg diet consumed approximately the same feed amount giving the same FCR (1.30-1.33). Furthermore, energy utilization decreased significantly at 2.0-5.0 g RCP/kg. No significant differences were observed in fish survival and its range was 93.3-96.7% among the different treatments. The supplementation of RCP reduced significantly protein and lipid contents and improved significantly ash content in whole-fish body. Furthermore, RCP inclusion resulted in significant decreases in plasmatic glucose, protein, and lipids, whereas their highest values were obtained with fish fed the control diet. Contrarily, plasmatic AST, ALT, creatinine, and uric acid values increased significantly and nitroblue tetrazolium (NBT) was significantly higher at RCP levels over 1.0 g/kg diet. After Zn exposure, Zn effect was more severe in fish fed RCP-free diet than those fed RCP-enrich diets. In control Zn-exposed fish, plasmatic glucose, total protein, and total lipids were significantly higher; meanwhile, plasmatic AST, ALT, creatinine, and uric acid levels were lower than those in fish fed RCP levels. In addition, NBT decreased due to Zn exposure. Likewise, Zn residues in whole-fish body decreased significantly with increasing RCP levels in diets and lowest daily Zn content was detected in fish fed 2.0-5.0 g RCP/kg diet. These results suggested that RCP supplementation cannot improve fish growth and feed utilization but it could improve their immunity and reduce the impact of water-born Zn toxicity and bioaccumulation in fish body.

The potential ability of the electric field to produce genotoxic effect is still a matter of deba... more The potential ability of the electric field to produce genotoxic effect is still a matter of debate. Indeed limited data are available about chromosomal aberrtions and other genetic alternations after exposure to electric field.

garlic has been used as a medicinal agent for thousands of years

International Journal of Basic and Applied Sciences, 2014

Profenofos as an organophosphorus insecticide has been used in the agricultural countries as Egyp... more Profenofos as an organophosphorus insecticide has been used in the agricultural countries as Egypt, may find its way to water system and adversely effect on aquatic life particularly fish. Nile tilapia, Oreochromis niloticus as a major fish species in River Nile and one of the major sources of protein for human beings in Egypt, and it can also be a source of threaten to human health. Transport profenofos directly to tilapia fish may affect their physiological status and then fish production. The mortality of profenofos toxicity was estimated on tilapia and LC50 was detected as 0.87 mg/l. Also fish were exposed to 1/2 LC50 for 96 hrs and to 1/10 LC50 for 28 days and lastly were left after the chronic toxicity for another 28 days as recovery period. The increase of blood glucose was accompanied with decrease in liver and muscles glycogen throughout the acute and chronic trail periods. Fish showed also highly significant decrease in serum total protein and globulin with increasing in albumin and A/G ratio. A sharp elevation in serum creatinine, urea and uric acid with decrease in serum total lipid, triglyceride and cholesterol were also recorded. Lastly gradual and sharp elevation in the levels of serum enzymes, S-AST, S-ALT and S-ALP was revealed in profenofos-exposed tilapia. Same behavior as S-AST and S-ALT were in liver transaminases (L-AST & L-ALT). Our study revealed adverse change of metabolism in tilapia due to profenofos exposure. This may inform about the dangerous use of profenofos and limitations should be managed.

faculty.ksu.edu.sa

Liver specimens were taken from seventy fish, Tilapia zillii, living in 3 ecologically different ... more Liver specimens were taken from seventy fish, Tilapia zillii, living in 3 ecologically different localities in Qalyobya governorate, i.e., unpolluted, agricultural-polluted and industrial-polluted waters of River Nile. Water analysis was carried out to evaluate the heavy metals in these tested localities. Polymorphism of liver proteins was revealed by SDS-PAGE electrophoresis. Also, DNA damage was detected by counting the fragmentation percent. Results showed that the measured heavy metals were higher in the agricultural and industrial localities than in control; this was to extent that crossed the permissible concentrations. Fractions of liver proteins showed disappearance and polymorphism in the fish especially that was very close to the outlets of agricultural and industrial drainage. Additionally, both agricultural and industrial water pollution caused an increase in the percentage of DNA fragmentation however the percentage of industrial pollution was higher than in agricultural pollution.

zoology.eajbs.eg.net

Shanawan drainage canal is one of the many drainage canals present in Menufiya province, Egypt. I... more Shanawan drainage canal is one of the many drainage canals present in Menufiya province, Egypt. It receives sewage water from Kafr Shanawan drainage canal. Also, it receive illegal run-off from sewage and wastes besides sewage of Shebeen Al-koom city. This study is aimed to investigate the frequency of chromosomal damage by using chromosomal aberrations test of head kidney cells in Oreochromis niloticus and Tilapia zillii fish. The study was performed on 5 groups of fish, control and four polluted groups. A and B stations are located before the source of sewage discharge while C and D stations are located after the source of sewage. In each group cytological studies were performed. Fishes caught from areas before the sources of sewage showing different types of chromosomal aberrations, including centromeric attenuations, chromatid breaks, chromatid gabs, chromatid deletion, centric fusion and fragmentation. The total aberrations were more obvious among the fishes of the areas after the source of sewage than those of the areas before the source of sewage. It was noted that O. niloticus is more sensitive to the effect of pollution than T. zillii. Cytogenetic Studies of the current Work could be used as criteria for pollution intensity, which can be used to avoid its toxic effect on aquatic environment.

faculty.ksu.edu.sa

The potential ability of the electric field to produce genotoxic effect is still a matter of deba... more The potential ability of the electric field to produce genotoxic effect is still a matter of debate. Indeed, limited data are available about chromosomal aberrations and other genetic alternations after exposure to electric field. Fourty five mature female albino rats Rattus norvegicus were subjected as three groups. Rats were exposed to continuous microwave (MW) radiation of 9.865 GHz for 30, 45 and 60 days. The same intervals were studied to show the effect of garlic, Allium sativum treatment in association with the MW exposure. Results showed a significant induction of the structural chromosomal aberrations; deletion, break, gap, end to end association, centromeric attenuation, ring chromosome and stickness in the bone marrow cells. Administration of 20mg/kg garlic daily suppressed in significant way the occurred aberrations. The present work suggested the in vivo radioprotective role and antimutagenic effect of garlic. Chromosome aberrations as biomarkers of radiation exposure: modelling basic mechanisms. Adv. Space Res., 31(6): 1557-1568. Banerjee, R.; Goldfeder, A. and Mitra, J. (1983): Sister chromatid exchanges and chromosome aberrations induced by radiosensitizing agents in bone marrow cells of treated tumorbearing mice. J. Natl. Cancer Inst., 70(3): 517-521. Baranski, S. and Czerski, P. (1976): Biological effects of microwaves, Dowen, Hutchinson and Ross, New York. Bauchinger, M.; Hauf, R.; Schmid, E. and Dresp, J. (1981): Analysis of structural chromosome changes and SCE after occupational long-term exposure to electric and magnetic fields from KV-systems, Radiat. Environ. Biophys., 19: 235-238. Blackman, C.F. (1990): "ELF effects on calcium homeostasis". In"Extremely low frequency electromagnetic fields: The question of cancer", BW Wilson, RG Stevens, LE Anderson Eds, Publ. Battelle Press Columbus: 187-208. Cohen, M.M.; Kunska, A.; Astemborski, J.A.; McCulloch, D. and Paskewitz, D.A. (1986): Effect of low-level, 60-Hz electromagnetic fields on human lymphoid cells: I. Mitotic rate and chromosome breakage in human peripheral lymphocytes. Bioelectr., 7(4): 415-423. Cooke, P. and Morris, P.G. (1981): 12 The effects of NMR exposure on living organisms, . A genetic study of human lymphocytes. Br. J. Radiol., 45: 622-625. D`Ambrosio, G.; Massa, R.; Di Berardino, D.; Lioi, M.B.; Scaglione, A. and Scarfi, M.R. (1988): Chromosomal aberrations in Bovine lymphocytes exposed to 50-Hz electric currents. J. Bioelectr., 7(2): 239-245. D`Ambrosio, G.; Secaglione, A.; Dibernardino, D.; Lioi, M.B.; Lannuzzi, L; Mostacciuolo, E. and Scarfi, M.R. (1985): Chromosomal aberrations induced by FLF electric fields. J. Bioelect., 4: 279-284.

The present study was carried out to investigate the effect of sublethal zinc (Zn) concentrations... more The present study was carried out to investigate the effect of sublethal zinc (Zn)
concentrations on the growth, biochemical variables, and Zn residues in common
carp, Cyprinus carpio L.. Fish (18.1 – 19.1 g) were exposed to 0.0 (control), 5.0 and
10.0 mg Zn/L for 7, 14, 28, and 56 days. At each time interval and each treatment,
fish were collected, weighed and sampled to measure the growth, biochemical
variables, and Zn residues in whole-fish body. Growth performance was significantly
reduced with increasing Zn concentrations. However, fish exposed to 10.0 mg Zn/L
for 56 days grew lower than that of the control group. Likewise, the optimum feed
intake and feed conversion ratio were obtained at control group at 56 days.
Furthermore, glucose, AST, ALT, creatinine, and cortisol increased significantly with
increasing Zn concentration and exposure time, with maximal values at 56 days.
Meanwhile, the highest values of serum protein and lipids of were obtained in the
control fish reared for 56 days, whereas the lowest values were observed in fish
exposed to 10.0 mg Zn/L for 56 days. The content of whole-body moisture and total
ash increased significantly, while crude protein and total lipid contents decreased
significantly with increasing Zn concentrations. In addition, Zn exposure increased Zn
residues in fish body; however, Zn bioaccumulation in fish body was Zn dose and
time dependant. The present study revealed that the growth and health status of
common carp were deteriorated by Zn toxicity.

The effect of acute and chronic exposure of pesticide profenofos on its accumulation and some hem... more The effect of acute and chronic exposure of pesticide profenofos on its accumulation and some hematological parameters of the Nile tilapia (Oreochromis niloticus) were studied. The 96 hrs LC 50 of profenofos to Nile tilapia was determined to be 0.87 mg/l. No detectable profenofos in aquaria water was observed after 14, 21 and 28 days of trial period and during recovery. Profenofos were accumulated in higher rate fish tissues during the acute exposure. However, during chronic period a gradual increase was observed through 1 to 7 days followed by gradual decrease until it was not detectable at the end of 28 days. Regarding hematology, highly significant increase in WBCs counts during both the acute and chronic exposure to was observed. Highly significant decrease in RBCs counts, Hb content and Hct % was noticed during experimental periods. For most of the recorded data, it showed marked improvement during the recovery period. There was a significant increase in MCHC during both acute ...

Aquaculture, 2015

The present study was undertaken to evaluate the use of roasted coffee powder (RCP; Coffea arabic... more The present study was undertaken to evaluate the use of roasted coffee powder (RCP; Coffea arabica) in practical diets for common carp, Cyprinus carpio L. to improve their growth, biochemical status, and resistance against Zn toxicity. However, RCP was added to the ingredients of tested diets to represent 0.0 (control), 0.50, 1.0, 2.0, or 5.0 g/kg diet. Fish (10.2 ± 0.42 g) were distributed into various treatments at a rate of 20 fish per 100-L aquarium and fed one of the experimental diets for 10 weeks in triplicates. After the feeding trial, fish from each treatment were further-exposed to 5.0 mg Zn/L for 7 days. It is noticed that final fish performance was not significantly (P b 0.05) affected by increasing RCP levels up to 1.0 g/kg after which fish growth declined. Moreover, fish fed diets containing 2.0-5.0 g RCP/kg consumed less diet than the other treatments giving highest FCRs (1.46 and 1.53, respectively), whereas fish fed 0.0-1.0 RCP/kg diet consumed approximately the same feed amount giving the same FCR (1.30-1.33). Furthermore, energy utilization decreased significantly at 2.0-5.0 g RCP/kg. No significant differences were observed in fish survival and its range was 93.3-96.7% among the different treatments. The supplementation of RCP reduced significantly protein and lipid contents and improved significantly ash content in whole-fish body. Furthermore, RCP inclusion resulted in significant decreases in plasmatic glucose, protein, and lipids, whereas their highest values were obtained with fish fed the control diet. Contrarily, plasmatic AST, ALT, creatinine, and uric acid values increased significantly and nitroblue tetrazolium (NBT) was significantly higher at RCP levels over 1.0 g/kg diet. After Zn exposure, Zn effect was more severe in fish fed RCP-free diet than those fed RCP-enrich diets. In control Zn-exposed fish, plasmatic glucose, total protein, and total lipids were significantly higher; meanwhile, plasmatic AST, ALT, creatinine, and uric acid levels were lower than those in fish fed RCP levels. In addition, NBT decreased due to Zn exposure. Likewise, Zn residues in whole-fish body decreased significantly with increasing RCP levels in diets and lowest daily Zn content was detected in fish fed 2.0-5.0 g RCP/kg diet. These results suggested that RCP supplementation cannot improve fish growth and feed utilization but it could improve their immunity and reduce the impact of water-born Zn toxicity and bioaccumulation in fish body.

The potential ability of the electric field to produce genotoxic effect is still a matter of deba... more The potential ability of the electric field to produce genotoxic effect is still a matter of debate. Indeed limited data are available about chromosomal aberrtions and other genetic alternations after exposure to electric field.

garlic has been used as a medicinal agent for thousands of years

International Journal of Basic and Applied Sciences, 2014

Profenofos as an organophosphorus insecticide has been used in the agricultural countries as Egyp... more Profenofos as an organophosphorus insecticide has been used in the agricultural countries as Egypt, may find its way to water system and adversely effect on aquatic life particularly fish. Nile tilapia, Oreochromis niloticus as a major fish species in River Nile and one of the major sources of protein for human beings in Egypt, and it can also be a source of threaten to human health. Transport profenofos directly to tilapia fish may affect their physiological status and then fish production. The mortality of profenofos toxicity was estimated on tilapia and LC50 was detected as 0.87 mg/l. Also fish were exposed to 1/2 LC50 for 96 hrs and to 1/10 LC50 for 28 days and lastly were left after the chronic toxicity for another 28 days as recovery period. The increase of blood glucose was accompanied with decrease in liver and muscles glycogen throughout the acute and chronic trail periods. Fish showed also highly significant decrease in serum total protein and globulin with increasing in albumin and A/G ratio. A sharp elevation in serum creatinine, urea and uric acid with decrease in serum total lipid, triglyceride and cholesterol were also recorded. Lastly gradual and sharp elevation in the levels of serum enzymes, S-AST, S-ALT and S-ALP was revealed in profenofos-exposed tilapia. Same behavior as S-AST and S-ALT were in liver transaminases (L-AST & L-ALT). Our study revealed adverse change of metabolism in tilapia due to profenofos exposure. This may inform about the dangerous use of profenofos and limitations should be managed.

faculty.ksu.edu.sa

Liver specimens were taken from seventy fish, Tilapia zillii, living in 3 ecologically different ... more Liver specimens were taken from seventy fish, Tilapia zillii, living in 3 ecologically different localities in Qalyobya governorate, i.e., unpolluted, agricultural-polluted and industrial-polluted waters of River Nile. Water analysis was carried out to evaluate the heavy metals in these tested localities. Polymorphism of liver proteins was revealed by SDS-PAGE electrophoresis. Also, DNA damage was detected by counting the fragmentation percent. Results showed that the measured heavy metals were higher in the agricultural and industrial localities than in control; this was to extent that crossed the permissible concentrations. Fractions of liver proteins showed disappearance and polymorphism in the fish especially that was very close to the outlets of agricultural and industrial drainage. Additionally, both agricultural and industrial water pollution caused an increase in the percentage of DNA fragmentation however the percentage of industrial pollution was higher than in agricultural pollution.

zoology.eajbs.eg.net

Shanawan drainage canal is one of the many drainage canals present in Menufiya province, Egypt. I... more Shanawan drainage canal is one of the many drainage canals present in Menufiya province, Egypt. It receives sewage water from Kafr Shanawan drainage canal. Also, it receive illegal run-off from sewage and wastes besides sewage of Shebeen Al-koom city. This study is aimed to investigate the frequency of chromosomal damage by using chromosomal aberrations test of head kidney cells in Oreochromis niloticus and Tilapia zillii fish. The study was performed on 5 groups of fish, control and four polluted groups. A and B stations are located before the source of sewage discharge while C and D stations are located after the source of sewage. In each group cytological studies were performed. Fishes caught from areas before the sources of sewage showing different types of chromosomal aberrations, including centromeric attenuations, chromatid breaks, chromatid gabs, chromatid deletion, centric fusion and fragmentation. The total aberrations were more obvious among the fishes of the areas after the source of sewage than those of the areas before the source of sewage. It was noted that O. niloticus is more sensitive to the effect of pollution than T. zillii. Cytogenetic Studies of the current Work could be used as criteria for pollution intensity, which can be used to avoid its toxic effect on aquatic environment.

faculty.ksu.edu.sa

The potential ability of the electric field to produce genotoxic effect is still a matter of deba... more The potential ability of the electric field to produce genotoxic effect is still a matter of debate. Indeed, limited data are available about chromosomal aberrations and other genetic alternations after exposure to electric field. Fourty five mature female albino rats Rattus norvegicus were subjected as three groups. Rats were exposed to continuous microwave (MW) radiation of 9.865 GHz for 30, 45 and 60 days. The same intervals were studied to show the effect of garlic, Allium sativum treatment in association with the MW exposure. Results showed a significant induction of the structural chromosomal aberrations; deletion, break, gap, end to end association, centromeric attenuation, ring chromosome and stickness in the bone marrow cells. Administration of 20mg/kg garlic daily suppressed in significant way the occurred aberrations. The present work suggested the in vivo radioprotective role and antimutagenic effect of garlic. Chromosome aberrations as biomarkers of radiation exposure: modelling basic mechanisms. Adv. Space Res., 31(6): 1557-1568. Banerjee, R.; Goldfeder, A. and Mitra, J. (1983): Sister chromatid exchanges and chromosome aberrations induced by radiosensitizing agents in bone marrow cells of treated tumorbearing mice. J. Natl. Cancer Inst., 70(3): 517-521. Baranski, S. and Czerski, P. (1976): Biological effects of microwaves, Dowen, Hutchinson and Ross, New York. Bauchinger, M.; Hauf, R.; Schmid, E. and Dresp, J. (1981): Analysis of structural chromosome changes and SCE after occupational long-term exposure to electric and magnetic fields from KV-systems, Radiat. Environ. Biophys., 19: 235-238. Blackman, C.F. (1990): "ELF effects on calcium homeostasis". In"Extremely low frequency electromagnetic fields: The question of cancer", BW Wilson, RG Stevens, LE Anderson Eds, Publ. Battelle Press Columbus: 187-208. Cohen, M.M.; Kunska, A.; Astemborski, J.A.; McCulloch, D. and Paskewitz, D.A. (1986): Effect of low-level, 60-Hz electromagnetic fields on human lymphoid cells: I. Mitotic rate and chromosome breakage in human peripheral lymphocytes. Bioelectr., 7(4): 415-423. Cooke, P. and Morris, P.G. (1981): 12 The effects of NMR exposure on living organisms, . A genetic study of human lymphocytes. Br. J. Radiol., 45: 622-625. D`Ambrosio, G.; Massa, R.; Di Berardino, D.; Lioi, M.B.; Scaglione, A. and Scarfi, M.R. (1988): Chromosomal aberrations in Bovine lymphocytes exposed to 50-Hz electric currents. J. Bioelectr., 7(2): 239-245. D`Ambrosio, G.; Secaglione, A.; Dibernardino, D.; Lioi, M.B.; Lannuzzi, L; Mostacciuolo, E. and Scarfi, M.R. (1985): Chromosomal aberrations induced by FLF electric fields. J. Bioelect., 4: 279-284.

The present study was carried out to investigate the effect of sublethal zinc (Zn) concentrations... more The present study was carried out to investigate the effect of sublethal zinc (Zn)
concentrations on the growth, biochemical variables, and Zn residues in common
carp, Cyprinus carpio L.. Fish (18.1 – 19.1 g) were exposed to 0.0 (control), 5.0 and
10.0 mg Zn/L for 7, 14, 28, and 56 days. At each time interval and each treatment,
fish were collected, weighed and sampled to measure the growth, biochemical
variables, and Zn residues in whole-fish body. Growth performance was significantly
reduced with increasing Zn concentrations. However, fish exposed to 10.0 mg Zn/L
for 56 days grew lower than that of the control group. Likewise, the optimum feed
intake and feed conversion ratio were obtained at control group at 56 days.
Furthermore, glucose, AST, ALT, creatinine, and cortisol increased significantly with
increasing Zn concentration and exposure time, with maximal values at 56 days.
Meanwhile, the highest values of serum protein and lipids of were obtained in the
control fish reared for 56 days, whereas the lowest values were observed in fish
exposed to 10.0 mg Zn/L for 56 days. The content of whole-body moisture and total
ash increased significantly, while crude protein and total lipid contents decreased
significantly with increasing Zn concentrations. In addition, Zn exposure increased Zn
residues in fish body; however, Zn bioaccumulation in fish body was Zn dose and
time dependant. The present study revealed that the growth and health status of
common carp were deteriorated by Zn toxicity.