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Papers by Khaleel Basha

Journal of Umm Al-Qura University for Applied Sciences

The present work reports an efficient removal of a cationic dye, methylene blue (MB), and an anio... more The present work reports an efficient removal of a cationic dye, methylene blue (MB), and an anionic dye, methyl orange (MO) dye from an aqueous solution using graphene oxide (GO)–based nanocomposite as an adsorbent. GO was investigated as a potential nano-reinforcing filler in starch/poly(vinyl alcohol) (PVA) biopolymer matrix. Bio-nanocomposite based on starch/PVA matrix and GO were prepared by an aqueous casting method. The fabricated nanocomposites were characterized using FT-IR, XRD, Raman, TEM, FE-SEM, tensile study, Brunauer–Emmett–Teller (BET) method, Barrett–Joyner–Halenda (BJH) method, zeta potential, and swelling study. The effect of the various compositions of GO nanofiller in the starch/PVA matrix was highlighted and the impact of GO nanosheets on the properties of the nanocomposites was revealed. The results demonstrated that the starch/PVA matrix with 3 g of GO was found to be the optimal concentration of GO. Batch adsorption experiments were conducted to optimize the...

International Journal of Biological Macromolecules

Polymer Bulletin, 2022

Nanotechnology based platforms have gained new insights into the development of effective modes o... more Nanotechnology based platforms have gained new insights into the development of effective modes of drug delivery systems for addressing wounds and related pathologies. Drugs encapsulated in nanodimensioned materials or nanoparticles are becoming a dermatologically attractive and versatile strategy for the development of optimized pharmaceutical formulations. In the current study, we developed gel formulations of Quercetin (Q) loaded alginate (ALG)/chitosan nanoparticle (CSNP) with concentrations 0.01% and 0.075% incorporated into carbopol encoded as Q-ALG/CSNP-G1, Q-ALG/CSNP-G2, respectively, and assessed their wound healing potential when topically applied to open excision wounds on adult Wistar rats. The characterization tests confirmed Q-ALG/CSNP-G2 featured pH, spreadability, extrudability and consistency. The in vitro release profile showed that the optimized Q-ALG/CSNP-G2 released quercetin in a sustained manner of 62.51 ± 0.72% over the period of 24 h as optimally needed for the wound healing onrush covering the inflammatory and proliferative phases. The in vivo acute dermal toxicity study did not produce any overt indications of toxicity as compared with control rats. The healing time for wounds treated with quercetin was even longer than those treated with Q-ALG/CSNP-G2. Antioxidant assays (SOD, CAT, LPO, and NO) revealed enhanced free radical scavenging ability of Q-ALG/CSNP-G2 gel receiving rats, thus improving healing quality. Furthermore, the restoration of biomarkers hydroxyproline and hexosamine content significantly proved increased re-epithelialization and collagen formation. The histopathological investigations on wounds treated with drug-loaded gel demonstrated efficient healing, as evidenced by the deficit of inflammation, established fibrous tissues, well-organized fibroblasts, and blood capillaries. Combining the unique properties of controlled drug release, enhanced antioxidant and antibacterial effects, the developed Q-ALG/CSNP-G2 were topically effective and showed synergistic wound healing capabilities compared with free quercetin in Wistar albino rats.

Journal of Drug Delivery Science and Technology, 2020

Tissue engineering is the immense area of research in recent years because of its vast potential ... more Tissue engineering is the immense area of research in recent years because of its vast potential in the repair or replacement of impaired tissues and organs. Bone is a nanomaterial composed of organic (collagen) and inorganic (mainly nano-hydroxyapatite) components, with a hierarchical structure ranging from nanoscale to macroscale. The bone disorder has been increasing and the goal is to restore and improve the function of bone tissue by scaffolds, providing a suitable environment for tissue regeneration and repair. In this review biopolymeric nanocomposites provide a closer structural support approximation to native bone architecture for the cells and regulate cell proliferation, differentiation, and migration, which results in bone regeneration. Furthermore, there are some new challenges about the future research on the application of biopolymeric nanocomposites as scaffolds in the bone regeneration.

Current Drug Research Reviews, 2020

Background: Objective:: The stability and delivery of drugs remain one of the key hurdles in the ... more Background: Objective:: The stability and delivery of drugs remain one of the key hurdles in the present situation. The present study depends on the design of a novel nanoemulsion drugdelivery system that would encapsulate a drug and to improve drug stability. The charisma of nanotechnology is majorly due to the smallest particle size at the nanoscale. Methods: Nanoemulsions attention is focused on emphasizing formulation aspect, method of preparation characterization techniques, evaluation parameters and various application of the nanoemulsions, several techniques to be used for the preparation of nanoemulsions like microfluidization, high-pressure homogenization, low energy emulsification and solvent evaporation method and their parameters to be characterized. Results: The design of effective formulations for drugs is being applied to enhance the solubility and bioavailability of water-insoluble drugs. The nanosized droplets have led to considerable attraction for this formulation...

Asian Pacific Journal of Tropical Disease, 2012

Journal of Applied Chemical Science International, Jun 7, 2022

Materials Today: Proceedings, 2020

This review examines the large polysaccharide particles play in functional drug delivery. The sig... more This review examines the large polysaccharide particles play in functional drug delivery. The significance of these frameworks is because of the wide assortment of polysaccharides and their typical source which implies that they can achieve biocompatible and biodegradable systems with a scope of biological and chemical usefulness benefits critical to drug tracking / delivery. This value incorporates giving and presenting specialists working through shirk of the reticuloendothelial framework, regulating biomacromolecules, and growing the bioavailability of incorporated small molecule drugs. Transport of the helpful is likewise key basic for the utilization of polysaccharide particles, moves prescriptions from the site of organization through mucosal official and transport, and utilizing the science, size and receptor meddles sedate focusing to explicit tissues. This review also elaborates on techniques for integrating and developing frameworks for the use of high-affinity sedate transport polysaccharide molecules and extending the usefulness of normal polysaccharides.

Journal of Nanoscience and Nanotechnology, 2019

The present work reports low cost, green synthesis of Zinc oxide (ZnO) nanoparticles. The biosynt... more The present work reports low cost, green synthesis of Zinc oxide (ZnO) nanoparticles. The biosynthesized nanoparticles were characterized by transmission electron microscopy (TEM), X-ray diffraction (XRD) and Fourier transform infrared spectroscopy (ATR-FTIR). The synthesized ZnO nanoparticles were pure, predominantly spherical in shape with size ranging from 25 nm. The biosynthesized ZnO nanoparticles have been used for antibacterial and in vitro applications. The antibacterial activity of the prepared CS/PVA/MC3-ZnO1, CS/PVA/MC3-ZnO2, and CS/PVA/MC3-ZnO3 nanocomposites samples was tested against Staphylococcus aureus (S. aureus) and Escherichia coli (E. coli) due to disk diffusion method. When adding the ZnO nanoparticles both bacteria were very good inhibition zone was obtained. The transmission ZnO micrographs depicts spherical with uniform shape and good crystallinity and are composed of nanoparticles with a diameter less than 25 nm the average cube size was 100 nm. The AFM thickness of the CS/PVA/MC3-ZnO scaffold was, estimated from the AFM image, was about 10-20 nm and a roughness-like structure was observed. The FE-SEM film exhibits a scaffold exhibited porous structures. The excellent cell viability of the composite scaffolds was attributed to the good biocompatibility of the CS/PVA/MC3-ZnO3 as well as green fabrication process of the scaffolds. MTT analysis exposed that the samples did not have any toxicity. Since these positive points, these two kinds of scaffolds show appropriate properties for attachment, proliferation, and tendency to form group from L929 cells. In this work, we have prepared zinc oxide by high pressure homogenization process and the resultant zinc oxide was evaluated as fibers in CS/PVA/MC3 films.

Polymer Bulletin, 2017

In this study Ampicillin drug loaded with alginate and nanocellulose film was prepared by solutio... more In this study Ampicillin drug loaded with alginate and nanocellulose film was prepared by solution casting method. Nanocellulose and ampicillin incorporated into alginate to improve both mechanical and swelling property. The formulated ampicillin loaded Alg/NC film gave acceptable physicochemical properties compared with Alg-amp film and was able to deliver the drug in a prolonged release pattern. In vitro drug release showed that alginate, could provide an immediate release of ampicillin with further enhanced nanocellulose, and followed by a sustained release over 500 min of the remaining drug. The present study exhibited a simple and useful approach to systematically design for providing drug release profiles.

Journal of Nanoscience and Nanotechnology, 2018

Freeze drying and salt leaching methods were applied to fabricate Chitosan/Poly(vinyl alcohol)/Ca... more Freeze drying and salt leaching methods were applied to fabricate Chitosan/Poly(vinyl alcohol)/Carboxymethyl cellulose (CPCMC) biomimetic porous scaffolds for soft tissue engineering. The properties of these scaffolds were investigated and compared to those by freeze drying and salt leaching methods respectively. The salt-leached CS/PVA/CMC scaffolds were easily formed into desired shapes with a uniformly distributed and interconnected pore structure with an average pore size. The mechanical strength of the scaffolds increased with the porosity, and were easily modulated by the addition of carboxymethyl cellulose. The morphology of the porous scaffolds observed using a SEM exhibited good porosity and interconnectivity of pores. MTT assay using L929 fibroblast cells demonstrated that the cell viability of the porous scaffold was good. Scaffolds prepared by salt leached method show larger swelling capacity, and mechanical strength, potent antibacterial activity and more cell viability than freeze dried method. It is found that salt leaching method has distinguished characteristics of simple, efficient, feasible and less economic than freeze dried scaffolds.

International Journal of Biological Macromolecules, 2018

The present study was to develop a novel chitosan based symmetric and asymmetric bionanocomposite... more The present study was to develop a novel chitosan based symmetric and asymmetric bionanocomposite for potential wound dressing application. Chitosan (C)/Poly (vinyl pyrrolidone) (P)/nanocellulose (NC) membrane were fabricated by salt leaching method with the addition of 3% and 5% wt of nanocellulose. To obtain asymmetric material one side of the membrane was coated by stearic acid (S) which could form hydrophobic surface and another side acts as a hydrophilic surface. Nanocellulose of size 2-10 nm was synthesized and characterized by TEM analysis. SEM showed the hydrophilic surface of asymmetric bionanocomposite consists of porous structure and hydrophobic surface is smooth and homogeneous. The results revealed that the Chitosan/PVP/Nanocellulose 3%-Stearic acid (CPNC3%-S) had a moderate swelling ratio, porosity, barrier and mechanical properties. Incorporation of nanocellulose into chitosan/PVP matrix could enhance the antibacterial activity. The hydrophobic surface of the CPNC3%-S bionanocomposite shows water repellent and antiadhesion properties towards E.coli bacteria and also the hydrophilic surface exhibit excellent antibacterial property and cytotoxicity towards bacterial pathogens. In vivo wound healing test shows better re-epithelialization and wound contraction compared with control and Chitosan/PVP-stearic acid (CP-S) bionanocomposite. Asymmetric bionanocomposite Chitosan/PVP/Nanocellulose coated with 3%-Stearic acid (CPNC3%-S) exhibited very good invitro cytocompatibility and enabled a faster wound healing than symmetric dressing, hence showing great potential to be applied as wound dressings.

Sensing and Bio-Sensing Research, 2016

Zinc oxide nanoparticles was synthesized by alginate (A) through a rapid and a facile step in the... more Zinc oxide nanoparticles was synthesized by alginate (A) through a rapid and a facile step in the aqueous solution condition at room temperature. Fabrication of zinc oxide nanoparticles was characterized by ATR-FTIR, TEM and XRD. ATR-FTIR analysis confirmed that the A/ZnO NPs were encapsulated by the polymerized alginate. Their shape, structure and composition were assessed by SEM. TEM and XRD analysis indicated that the A/ZnO NPs give evidence of the crystalline nature of ZnO and hybrid NPs structure, which is suitable for ammonia gas sensor development. The controlled size of the A/ZnO NPs obtained using this innovative synthesis strategy minimizes the response time of 2-3 s to sense the ammonia gas significantly with a detection limit of 1 ppm were found at room temperature. The antibacterial tests revealed that the A/ZnO NPs exhibits a potent activity against gram positive and gram negative bacteria.

International Journal of Biological Macromolecules, 2018

Starch is an abundant, relatively inexpensive and ecofriendly materials which can be easily conve... more Starch is an abundant, relatively inexpensive and ecofriendly materials which can be easily convert into nanoparticle and also as filler for the preparation of bionanocomposite for wound dressing application. Symmetric and asymmetric Chitosan(C)/PVP(P) films containing porous structure supported with nanostarch (NS) were prepared by salt leaching method for wound dressing application. Symmetric Chitosan/PVP/Nanostarch (CPNS) film with 1% and 3% wt nanostarch was prepared without coating of stearic acid whereas asymmetric Chitosan/PVP/Nanostarch-Stearic acid (CPNS-S) film was prepared by coating of stearic acid. The stearic acid coated surface possesses hydrophobic water repellent, microporous, bacterial anti adhesion property and the stearic acid uncoated hydrophilic surface shows superior antibacterial and non cytotoxicity property with highly porous character. All the symmetric and asymmetric films exhibit almost same mechanical, barrier, swelling and hemolytic property reveals that the stearic acid does not affect the physical and hemolytic property whereas the concentration of nanostarch greatly influence the above property. The reinforcement of nanostarch with chitosan and PVP was proved by TEM and SEM analysis. The CPNS1%-S film shows excellent S.aureus anti adhesion property. Furthermore, the in vivo excision-type wound healing proved that the CPNS1%-S film enhanced the healing effect and increased reepithelialization and collagen formation.

Applied Surface Science, 2018

Polymeric scaffolds with nanocomposites could be used for biomedical applications In addition of ... more Polymeric scaffolds with nanocomposites could be used for biomedical applications In addition of NC nanoparticles to PVA/MC matrices to improve the tensile strength Nanocomposites were effective against Gram positive and Gram negative bacteria Synthesis and characterization of (NC Nps) a cytotoxicity and cell viability Induced by NC Nps on fibroblast L929 cell lines

International Journal on Applied Bio-Engineering, 2010

Polymer-Plastics Technology and Engineering, 2017

ABSTRACT Nanostarch, which is inexpensive and environmentally abundant, has been used as filler t... more ABSTRACT Nanostarch, which is inexpensive and environmentally abundant, has been used as filler to prepare biocompatible films. The bio-nanocomposites were synthesized by solution casting method. TEM analysis proves that the average particle size of the nanostarch is in the range of 20–40 nm. Thermal stability and cell viability of CS/PVP matrix were best enhanced at 1% of nanostarch loading. The antibacterial activity exhibited by CPS bio-nanocomposite against Staphylococcus aureus and Pseudomonas aeruginosa and synergistic effects of CPS such as barrier properties, swelling properties, and better blood compatibility make it a suitable material for in vitro wound healing application. GRAPHICAL ABSTRACT

Carbohydrate Polymers, 2015

SLN was prepared by a combined technique of solvent-diffusion and hot homogenization Chitosan... more SLN was prepared by a combined technique of solvent-diffusion and hot homogenization Chitosan coating stabilized SLN in simulated gastric condition Chitosan coating significantly improved mucoadhesive property of SLN Chitosan formed a thick layer around SLN in gastric condition as clearly observed by TEM Surfactants but not chitosan coating determined cell uptake of SLN A c c e p t e d M a n u s c r i p t 2 Solid lipid nanoparticles for oral drug delivery: chitosan coating improves stability, controlled delivery, mucoadhesion and cellular uptake

Journal of Umm Al-Qura University for Applied Sciences

The present work reports an efficient removal of a cationic dye, methylene blue (MB), and an anio... more The present work reports an efficient removal of a cationic dye, methylene blue (MB), and an anionic dye, methyl orange (MO) dye from an aqueous solution using graphene oxide (GO)–based nanocomposite as an adsorbent. GO was investigated as a potential nano-reinforcing filler in starch/poly(vinyl alcohol) (PVA) biopolymer matrix. Bio-nanocomposite based on starch/PVA matrix and GO were prepared by an aqueous casting method. The fabricated nanocomposites were characterized using FT-IR, XRD, Raman, TEM, FE-SEM, tensile study, Brunauer–Emmett–Teller (BET) method, Barrett–Joyner–Halenda (BJH) method, zeta potential, and swelling study. The effect of the various compositions of GO nanofiller in the starch/PVA matrix was highlighted and the impact of GO nanosheets on the properties of the nanocomposites was revealed. The results demonstrated that the starch/PVA matrix with 3 g of GO was found to be the optimal concentration of GO. Batch adsorption experiments were conducted to optimize the...

International Journal of Biological Macromolecules

Polymer Bulletin, 2022

Nanotechnology based platforms have gained new insights into the development of effective modes o... more Nanotechnology based platforms have gained new insights into the development of effective modes of drug delivery systems for addressing wounds and related pathologies. Drugs encapsulated in nanodimensioned materials or nanoparticles are becoming a dermatologically attractive and versatile strategy for the development of optimized pharmaceutical formulations. In the current study, we developed gel formulations of Quercetin (Q) loaded alginate (ALG)/chitosan nanoparticle (CSNP) with concentrations 0.01% and 0.075% incorporated into carbopol encoded as Q-ALG/CSNP-G1, Q-ALG/CSNP-G2, respectively, and assessed their wound healing potential when topically applied to open excision wounds on adult Wistar rats. The characterization tests confirmed Q-ALG/CSNP-G2 featured pH, spreadability, extrudability and consistency. The in vitro release profile showed that the optimized Q-ALG/CSNP-G2 released quercetin in a sustained manner of 62.51 ± 0.72% over the period of 24 h as optimally needed for the wound healing onrush covering the inflammatory and proliferative phases. The in vivo acute dermal toxicity study did not produce any overt indications of toxicity as compared with control rats. The healing time for wounds treated with quercetin was even longer than those treated with Q-ALG/CSNP-G2. Antioxidant assays (SOD, CAT, LPO, and NO) revealed enhanced free radical scavenging ability of Q-ALG/CSNP-G2 gel receiving rats, thus improving healing quality. Furthermore, the restoration of biomarkers hydroxyproline and hexosamine content significantly proved increased re-epithelialization and collagen formation. The histopathological investigations on wounds treated with drug-loaded gel demonstrated efficient healing, as evidenced by the deficit of inflammation, established fibrous tissues, well-organized fibroblasts, and blood capillaries. Combining the unique properties of controlled drug release, enhanced antioxidant and antibacterial effects, the developed Q-ALG/CSNP-G2 were topically effective and showed synergistic wound healing capabilities compared with free quercetin in Wistar albino rats.

Journal of Drug Delivery Science and Technology, 2020

Tissue engineering is the immense area of research in recent years because of its vast potential ... more Tissue engineering is the immense area of research in recent years because of its vast potential in the repair or replacement of impaired tissues and organs. Bone is a nanomaterial composed of organic (collagen) and inorganic (mainly nano-hydroxyapatite) components, with a hierarchical structure ranging from nanoscale to macroscale. The bone disorder has been increasing and the goal is to restore and improve the function of bone tissue by scaffolds, providing a suitable environment for tissue regeneration and repair. In this review biopolymeric nanocomposites provide a closer structural support approximation to native bone architecture for the cells and regulate cell proliferation, differentiation, and migration, which results in bone regeneration. Furthermore, there are some new challenges about the future research on the application of biopolymeric nanocomposites as scaffolds in the bone regeneration.

Current Drug Research Reviews, 2020

Background: Objective:: The stability and delivery of drugs remain one of the key hurdles in the ... more Background: Objective:: The stability and delivery of drugs remain one of the key hurdles in the present situation. The present study depends on the design of a novel nanoemulsion drugdelivery system that would encapsulate a drug and to improve drug stability. The charisma of nanotechnology is majorly due to the smallest particle size at the nanoscale. Methods: Nanoemulsions attention is focused on emphasizing formulation aspect, method of preparation characterization techniques, evaluation parameters and various application of the nanoemulsions, several techniques to be used for the preparation of nanoemulsions like microfluidization, high-pressure homogenization, low energy emulsification and solvent evaporation method and their parameters to be characterized. Results: The design of effective formulations for drugs is being applied to enhance the solubility and bioavailability of water-insoluble drugs. The nanosized droplets have led to considerable attraction for this formulation...

Asian Pacific Journal of Tropical Disease, 2012

Journal of Applied Chemical Science International, Jun 7, 2022

Materials Today: Proceedings, 2020

This review examines the large polysaccharide particles play in functional drug delivery. The sig... more This review examines the large polysaccharide particles play in functional drug delivery. The significance of these frameworks is because of the wide assortment of polysaccharides and their typical source which implies that they can achieve biocompatible and biodegradable systems with a scope of biological and chemical usefulness benefits critical to drug tracking / delivery. This value incorporates giving and presenting specialists working through shirk of the reticuloendothelial framework, regulating biomacromolecules, and growing the bioavailability of incorporated small molecule drugs. Transport of the helpful is likewise key basic for the utilization of polysaccharide particles, moves prescriptions from the site of organization through mucosal official and transport, and utilizing the science, size and receptor meddles sedate focusing to explicit tissues. This review also elaborates on techniques for integrating and developing frameworks for the use of high-affinity sedate transport polysaccharide molecules and extending the usefulness of normal polysaccharides.

Journal of Nanoscience and Nanotechnology, 2019

The present work reports low cost, green synthesis of Zinc oxide (ZnO) nanoparticles. The biosynt... more The present work reports low cost, green synthesis of Zinc oxide (ZnO) nanoparticles. The biosynthesized nanoparticles were characterized by transmission electron microscopy (TEM), X-ray diffraction (XRD) and Fourier transform infrared spectroscopy (ATR-FTIR). The synthesized ZnO nanoparticles were pure, predominantly spherical in shape with size ranging from 25 nm. The biosynthesized ZnO nanoparticles have been used for antibacterial and in vitro applications. The antibacterial activity of the prepared CS/PVA/MC3-ZnO1, CS/PVA/MC3-ZnO2, and CS/PVA/MC3-ZnO3 nanocomposites samples was tested against Staphylococcus aureus (S. aureus) and Escherichia coli (E. coli) due to disk diffusion method. When adding the ZnO nanoparticles both bacteria were very good inhibition zone was obtained. The transmission ZnO micrographs depicts spherical with uniform shape and good crystallinity and are composed of nanoparticles with a diameter less than 25 nm the average cube size was 100 nm. The AFM thickness of the CS/PVA/MC3-ZnO scaffold was, estimated from the AFM image, was about 10-20 nm and a roughness-like structure was observed. The FE-SEM film exhibits a scaffold exhibited porous structures. The excellent cell viability of the composite scaffolds was attributed to the good biocompatibility of the CS/PVA/MC3-ZnO3 as well as green fabrication process of the scaffolds. MTT analysis exposed that the samples did not have any toxicity. Since these positive points, these two kinds of scaffolds show appropriate properties for attachment, proliferation, and tendency to form group from L929 cells. In this work, we have prepared zinc oxide by high pressure homogenization process and the resultant zinc oxide was evaluated as fibers in CS/PVA/MC3 films.

Polymer Bulletin, 2017

In this study Ampicillin drug loaded with alginate and nanocellulose film was prepared by solutio... more In this study Ampicillin drug loaded with alginate and nanocellulose film was prepared by solution casting method. Nanocellulose and ampicillin incorporated into alginate to improve both mechanical and swelling property. The formulated ampicillin loaded Alg/NC film gave acceptable physicochemical properties compared with Alg-amp film and was able to deliver the drug in a prolonged release pattern. In vitro drug release showed that alginate, could provide an immediate release of ampicillin with further enhanced nanocellulose, and followed by a sustained release over 500 min of the remaining drug. The present study exhibited a simple and useful approach to systematically design for providing drug release profiles.

Journal of Nanoscience and Nanotechnology, 2018

Freeze drying and salt leaching methods were applied to fabricate Chitosan/Poly(vinyl alcohol)/Ca... more Freeze drying and salt leaching methods were applied to fabricate Chitosan/Poly(vinyl alcohol)/Carboxymethyl cellulose (CPCMC) biomimetic porous scaffolds for soft tissue engineering. The properties of these scaffolds were investigated and compared to those by freeze drying and salt leaching methods respectively. The salt-leached CS/PVA/CMC scaffolds were easily formed into desired shapes with a uniformly distributed and interconnected pore structure with an average pore size. The mechanical strength of the scaffolds increased with the porosity, and were easily modulated by the addition of carboxymethyl cellulose. The morphology of the porous scaffolds observed using a SEM exhibited good porosity and interconnectivity of pores. MTT assay using L929 fibroblast cells demonstrated that the cell viability of the porous scaffold was good. Scaffolds prepared by salt leached method show larger swelling capacity, and mechanical strength, potent antibacterial activity and more cell viability than freeze dried method. It is found that salt leaching method has distinguished characteristics of simple, efficient, feasible and less economic than freeze dried scaffolds.

International Journal of Biological Macromolecules, 2018

The present study was to develop a novel chitosan based symmetric and asymmetric bionanocomposite... more The present study was to develop a novel chitosan based symmetric and asymmetric bionanocomposite for potential wound dressing application. Chitosan (C)/Poly (vinyl pyrrolidone) (P)/nanocellulose (NC) membrane were fabricated by salt leaching method with the addition of 3% and 5% wt of nanocellulose. To obtain asymmetric material one side of the membrane was coated by stearic acid (S) which could form hydrophobic surface and another side acts as a hydrophilic surface. Nanocellulose of size 2-10 nm was synthesized and characterized by TEM analysis. SEM showed the hydrophilic surface of asymmetric bionanocomposite consists of porous structure and hydrophobic surface is smooth and homogeneous. The results revealed that the Chitosan/PVP/Nanocellulose 3%-Stearic acid (CPNC3%-S) had a moderate swelling ratio, porosity, barrier and mechanical properties. Incorporation of nanocellulose into chitosan/PVP matrix could enhance the antibacterial activity. The hydrophobic surface of the CPNC3%-S bionanocomposite shows water repellent and antiadhesion properties towards E.coli bacteria and also the hydrophilic surface exhibit excellent antibacterial property and cytotoxicity towards bacterial pathogens. In vivo wound healing test shows better re-epithelialization and wound contraction compared with control and Chitosan/PVP-stearic acid (CP-S) bionanocomposite. Asymmetric bionanocomposite Chitosan/PVP/Nanocellulose coated with 3%-Stearic acid (CPNC3%-S) exhibited very good invitro cytocompatibility and enabled a faster wound healing than symmetric dressing, hence showing great potential to be applied as wound dressings.

Sensing and Bio-Sensing Research, 2016

Zinc oxide nanoparticles was synthesized by alginate (A) through a rapid and a facile step in the... more Zinc oxide nanoparticles was synthesized by alginate (A) through a rapid and a facile step in the aqueous solution condition at room temperature. Fabrication of zinc oxide nanoparticles was characterized by ATR-FTIR, TEM and XRD. ATR-FTIR analysis confirmed that the A/ZnO NPs were encapsulated by the polymerized alginate. Their shape, structure and composition were assessed by SEM. TEM and XRD analysis indicated that the A/ZnO NPs give evidence of the crystalline nature of ZnO and hybrid NPs structure, which is suitable for ammonia gas sensor development. The controlled size of the A/ZnO NPs obtained using this innovative synthesis strategy minimizes the response time of 2-3 s to sense the ammonia gas significantly with a detection limit of 1 ppm were found at room temperature. The antibacterial tests revealed that the A/ZnO NPs exhibits a potent activity against gram positive and gram negative bacteria.

International Journal of Biological Macromolecules, 2018

Starch is an abundant, relatively inexpensive and ecofriendly materials which can be easily conve... more Starch is an abundant, relatively inexpensive and ecofriendly materials which can be easily convert into nanoparticle and also as filler for the preparation of bionanocomposite for wound dressing application. Symmetric and asymmetric Chitosan(C)/PVP(P) films containing porous structure supported with nanostarch (NS) were prepared by salt leaching method for wound dressing application. Symmetric Chitosan/PVP/Nanostarch (CPNS) film with 1% and 3% wt nanostarch was prepared without coating of stearic acid whereas asymmetric Chitosan/PVP/Nanostarch-Stearic acid (CPNS-S) film was prepared by coating of stearic acid. The stearic acid coated surface possesses hydrophobic water repellent, microporous, bacterial anti adhesion property and the stearic acid uncoated hydrophilic surface shows superior antibacterial and non cytotoxicity property with highly porous character. All the symmetric and asymmetric films exhibit almost same mechanical, barrier, swelling and hemolytic property reveals that the stearic acid does not affect the physical and hemolytic property whereas the concentration of nanostarch greatly influence the above property. The reinforcement of nanostarch with chitosan and PVP was proved by TEM and SEM analysis. The CPNS1%-S film shows excellent S.aureus anti adhesion property. Furthermore, the in vivo excision-type wound healing proved that the CPNS1%-S film enhanced the healing effect and increased reepithelialization and collagen formation.

Applied Surface Science, 2018

Polymeric scaffolds with nanocomposites could be used for biomedical applications In addition of ... more Polymeric scaffolds with nanocomposites could be used for biomedical applications In addition of NC nanoparticles to PVA/MC matrices to improve the tensile strength Nanocomposites were effective against Gram positive and Gram negative bacteria Synthesis and characterization of (NC Nps) a cytotoxicity and cell viability Induced by NC Nps on fibroblast L929 cell lines

International Journal on Applied Bio-Engineering, 2010

Polymer-Plastics Technology and Engineering, 2017

ABSTRACT Nanostarch, which is inexpensive and environmentally abundant, has been used as filler t... more ABSTRACT Nanostarch, which is inexpensive and environmentally abundant, has been used as filler to prepare biocompatible films. The bio-nanocomposites were synthesized by solution casting method. TEM analysis proves that the average particle size of the nanostarch is in the range of 20–40 nm. Thermal stability and cell viability of CS/PVP matrix were best enhanced at 1% of nanostarch loading. The antibacterial activity exhibited by CPS bio-nanocomposite against Staphylococcus aureus and Pseudomonas aeruginosa and synergistic effects of CPS such as barrier properties, swelling properties, and better blood compatibility make it a suitable material for in vitro wound healing application. GRAPHICAL ABSTRACT

Carbohydrate Polymers, 2015

SLN was prepared by a combined technique of solvent-diffusion and hot homogenization Chitosan... more SLN was prepared by a combined technique of solvent-diffusion and hot homogenization Chitosan coating stabilized SLN in simulated gastric condition Chitosan coating significantly improved mucoadhesive property of SLN Chitosan formed a thick layer around SLN in gastric condition as clearly observed by TEM Surfactants but not chitosan coating determined cell uptake of SLN A c c e p t e d M a n u s c r i p t 2 Solid lipid nanoparticles for oral drug delivery: chitosan coating improves stability, controlled delivery, mucoadhesion and cellular uptake