Md. Abdul Khaleque - Academia.edu (original) (raw)

Papers by Md. Abdul Khaleque

Research paper thumbnail of Liposome Immobilization on Cross-linked Polymer Gel by In Situ Formation of Cleavable Covalent Bonds

Journal of Bioactive and Compatible Polymers, Nov 1, 2006

Research paper thumbnail of Trace metals contamination potential and health risk assessment of commonly consumed fish of Perak River, Malaysia

Research paper thumbnail of The Application of Nanotechnology in Medical Sciences: New Horizon of Treatment

American Journal of Biomedical Sciences, 2017

Nanotechnology has become enormously promising technology during last few decades and now it is e... more Nanotechnology has become enormously promising technology during last few decades and now it is estimated to have a significant effect on medical equipment. The possible effects of novel nanomedical applications on sickness identification, treatment, as well as anticipation are expected to improve healthiness care greatly. In addition, therapeutic selection may gradually be adapted to all patients' profile. Applications of nanomaterials are reported in operation, cancer diagnosis as well as therapy, biodetection of sickness markers, molecular imaging, implant technology, tissue engineering, as well as devices for drug, protein, and gene and radionuclide release. Various medical nanotechnology applications are at a standstill in their immaturity. A larger amount of nanodevices are presently under medical examination as well as several devices are commercially available. In this review work some applications of nanodevices in medical technology are also summarized.

Research paper thumbnail of Waterlogging in the Southwest Coastal Areas of Bangladesh: Local Adaptation Techniques and Challenges

American Scientific Research Journal for Engineering, Technology, and Sciences, 2020

Waterlogging is a critical environmental problem in the southwest coast of Bangladesh predominant... more Waterlogging is a critical environmental problem in the southwest coast of Bangladesh predominantly in some parts of Jashore, Satkhira and Khulna districts which create substantial impacts on livelihood. Therefore, this research aims to analyze how the coastal society deals with the increasing pressure on livelihood that emerged during and after waterlogging episodes. This research is dependent on empirically gathered data using both quantitative and qualitative approaches. A total 210 in-depth interviews from six unions, two unions from each of the three districts were conducted using a semi-structured questionnaire. In addition, six focus group discussion sessions and six key informant interviews were organized. For statistical analysis of collected data, SPSS was used. The study identifies some critical impacts of waterlogging on livelihood which include physical and mental illness, crop failure and damage, disruption in cropping mechanism, loss of income, and increasing level of...

Research paper thumbnail of Chikungunya outbreak (2017) in Bangladesh: Clinical profile, economic impact and quality of life during the acute phase of the disease

PLoS neglected tropical diseases, Jun 1, 2018

Chikungunya virus causes mosquito-transmitted infection that leads to extensive morbidity affecti... more Chikungunya virus causes mosquito-transmitted infection that leads to extensive morbidity affecting substantial quality of life. Disease associated morbidity, quality of life, and financial loss are seldom reported in resources limited countries, such as Bangladesh. We reported the acute clinical profile, quality of life and consequent economic burden of the affected individuals in the recent chikungunya outbreak (May to September 2017) in Dhaka city, Bangladesh. We conducted a cross-sectional study during the peak of chikungunya outbreak (July 24 to August 5, 2017) to document the clinical profiles of confirmed cases (laboratory test positive) and probable cases diagnosed by medical practitioners. Data related to clinical symptoms, treatment cost, loss of productivity due to missing work days, and quality of life during their first two-weeks of symptom onset were collected via face to face interview using a structured questionnaire. World Health Organization endorsed questionnaire ...

Research paper thumbnail of A Study Of Environmental Impacts On The Coral Resources In The Vicinity Of The Saint Martin Island, Bangladesh

International Journal of Scientific & Technology Research, Jan 25, 2016

A study of the environmental impacts on the coral resources in the vicinity of the Saint Martin I... more A study of the environmental impacts on the coral resources in the vicinity of the Saint Martin Island, Bangladesh was conducted with a view to making an assessment of the current status of coral resources in the island and identifying major natural and anthropogenic environmental threats to the future sustainability of these resources. It is evident that the coral resources have been reduced significantly, and currently, only 41 coral species are available. The existing environmental condition (assessed by pH, salinity, turbidity and temperature) in the island is not found responsible for the survival of the corals. The study also reveals that the major anthropogenic interventions are responsible for the gradual depletion of the coral resources. The major anthropogenic threats to the coral resources are coral collection and overfishing. In addition, coral extraction is identified as a potential threat to the future integrity of coral communities in the island. Environmental threats from anthropogenic activities related to sedimentation, land erosion and pollution are also the concerns for the coral communities in the island. For the conservation of the coral population, no coral monitoring cell has been established in this island.

Research paper thumbnail of Sludge Management in the Textile Industries of Bangladesh: An Industrial Survey of the Impact of the 2015 Standards and Guidelines

Water

The textile sector of Bangladesh has positively contributed to a significant impact on its nation... more The textile sector of Bangladesh has positively contributed to a significant impact on its national economy and employment opportunities due to its rapid growth. The increasing number of wet processing units has led to a growing amount of wastewater volume as well as textile sludge (a byproduct of wastewater or effluent treatment plants). In 2015, the government of Bangladesh instituted the “Bangladesh Standards and Guidelines for Sludge Management”. Therefore, this case study aimed to assess these standards’ impact on the textile industry’s sludge management practices, informing academic scholars of the research opportunities available, and serving as a policymaking tool for various other South Asia and Southeast Asia economies. The sludge management situation of thirty-six industries (namely, twelve dyeing, twelve printing, and twelve washing) was herein assessed through a self-administered questionnaire survey of respondents from the respective ‘Top Management’ and ‘Environmental...

Research paper thumbnail of Removing Reactive Dyes from Textile Effluent Using Banana Fibre

The ability of a particular banana fibre in removing reactive dyes from textile wastewater was st... more The ability of a particular banana fibre in removing reactive dyes from textile wastewater was studied in the present work. Locally available ground seeded banana fibre of ≤ 212 μ was used as adsorbent. Initially the adsorbent was tested on standard Novacron Blue FN-R reactive dye solutions and then it was also applied to raw textile wastewater. The adsorbent was found very effective to remove reactive dyes from water. The adsorption was found dependent on pH, contact time, adsorbent/dye ratio and initial concentration of the dye solution. It was found that the adsorption efficiency became maximum at pH 2 and it decreased with increasing pH. The adsorption was quite fast; approximately 90% of removal efficiency was achieved in 5 m, and the adsorption was completed in 20 m under suitable conditions. It was observed that at adsorbent-dye ratio of 333:1, the adsorbent was able to eliminate the reactive dye completely from standard aqueous solution within 20 m at pH 2. The removal effic...

Research paper thumbnail of Liposome immobilization on polymer gel particles by in situ formation of covalent linkages

Research paper thumbnail of Liposome immobilization on cross-linked polymer gel by in situ formation of cleavable covalent bonds

Journal of bioactive and …, 2006

Research paper thumbnail of HOGLA LEAF AS A POTENTIAL BIO-ADSORBENT FOR THE TREATMENT OF REACTIVE DYES IN TEXTILE EFFLUENTS

A new bio-adsorbent to remove reactive dyes from textile effluent was investigated in the present... more A new bio-adsorbent to remove reactive dyes from textile effluent was investigated in the present study. The adsorbent was the leaves of locally available hogla plant (Typha angustata). Initially, sunfix yellow, a reactive dye widely used in textile effluents, was used to check the removal efficiency in terms of contact time, pH of dye solution and adsorbent dosage. Complete removal (100%) of dye was achieved at adsorbent/dye ratio of 2300:1 at pH 10 with 180 minutes contact time. The adsorbent was then applied to deep colored, raw textile wastewater samples and it was found that 2.3 g of adsorbent was able to convert 100 mL of deep colored wastewater to transparent water at pH 10. Additionally, treatment by the adsorbent resulted in significant decreases in pH, BOD, COD, TS, TDS and TSS of wastewater, while improving the DO level.

Research paper thumbnail of An Investigation Into The Water Quality Of Buriganga -A River Running Through Dhaka

Buriganga river is used for bathing, drinking, irrigation and industrial purposes and is consider... more Buriganga river is used for bathing, drinking, irrigation and industrial purposes and is considered to be the lifeline of Dhaka city. The water quality of Buriganga has become a matter of concern due to serious levels of pollution. The objective of the study was to determine the water quality of the selected section of Buriganga river which passes through Dhaka city. The water quality parameters were sampled during different seasons (summer, winter and autumn) and in 10 different sampling points along the river along the banks of the Buriganga River. The water quality parameters studied for this study were dissolved oxygen (DO), biochemical oxygen demand (BOD), chemical oxygen demand (COD), pH, turbidity, conductivity, total dissolved solids (TDS), nitrate and phosphate. The results showed that DO, BOD, COD, TDS, turbidity, nitrate and phosphate are at an alarming level and a discussion on the possible sources of the pollution are presented.

Research paper thumbnail of JNN

Fabrication of a hybrid consists of gold nanoparticles and multi walled carbon nanotubes (MWCNTs)... more Fabrication of a hybrid consists of gold nanoparticles and multi walled carbon nanotubes (MWCNTs) with the help of poly (amino acid) was investigated. Poly(acryloyl -alanine) was synthesized by precipitation polymerization in tetrahydrofuran. The polymers were used to form hybrids with MWCNTs in aqueous media. Subsequently, the polymer functionalized MWCNTs were fabricated by in situ formed gold nanoparticles. The fabrication by gold nanoparticles was confirmed by transmission electron microscopic analyses. The fabrication was attempted with different concentrations of lithium auric chloride solutions in the range of 0.1-1.2 mM in water. The lower concentration of the gold precursor solution resulted in the formation and attachment of gold nanoparticles without aggregation while the higher concentration above 1.0 mM led to the aggregation of gold nanoparticles. The gold nanoparticles were observed only on the surface of MWCNTs and none was in the bulk aqueous phase.

Research paper thumbnail of 2016-CEJ-Online

Research paper thumbnail of CEJ-2014

Research paper thumbnail of EPDM Rubber

Conducting rubber composites of ethylene propylene diene M-class rubber (EPDM) were prepared with... more Conducting rubber composites of ethylene propylene diene M-class rubber (EPDM) were prepared with multi walled carbon nanotubes (MWCNTs) and organo-clay, Cloisite Ò 15A to develop flexible strain sensitive materials. The composites were prepared with a constant amount of organo-clay and various amounts of MWCNTs from 5 to 50 wt.%. Organo-clay led to improved dispersion of MWCNTs in the rubber matrix. Upon increasing the MWCNT contents, the tensile strength, stiffness and electrical conductivity of the composites increased significantly but the elongation at break decreased in the EPDM/MWCNT composites. The organo-clay was able to improve tensile strength, stiffness, elongation at break and electrical conductivity of the composites. The non-symmetric linear resistance change was observed by the composites under deformation.

Research paper thumbnail of Paper 4

Immobilization of liposomes onto chemically modified Sephacryl gel particles by in situ reaction ... more Immobilization of liposomes onto chemically modified Sephacryl gel particles by in situ reaction between liposome-incorporated thiols and mercapto moieties on the gel to form disulfide linkages was investigated. For the immobilization, both the mercapto moieties and the incorporated thiol were essential. The immobilization occurred upon coincubation of the modified liposomes with the modified gel for 48 hours. Once immobilized, no spontaneous detachment of the immobilized liposomes was observed. The degree of immobilization depended on both the thiol content and the ratio of the liposomes to the gel partilces. In a typical immobilization with 25 mol% 1-octanethiol, 82% of the liposomal phosphatidylcholine in the system was found to be associated with the gel. By decreasing the ratio of the liposomes to gel it was possible to bring the immobilization close to quantitative one. Among the three different thiols examined (1-octanethiol, 1-hexadecanethiol and thiocholesterol), the extent of the immobilization was slightly higher with thiocholesterol than the alkanethiols. The immobilized liposomes were detached from the gel with dithiothreitol. Approximately 60% of the fluorescent dextran derivative encapsulated in the liposomes was retained throughout the immobilization-detachment process. The gel left after the detachment remained active for immobilizing a fresh batch of thiol-liposomes.

Research paper thumbnail of Paper 4

Immobilization of liposomes onto chemically modified Sephacryl gel particles by in situ reaction ... more Immobilization of liposomes onto chemically modified Sephacryl gel particles by in situ reaction between liposome-incorporated thiols and mercapto moieties on the gel to form disulfide linkages was investigated. For the immobilization, both the mercapto moieties and the incorporated thiol were essential. The immobilization occurred upon coincubation of the modified liposomes with the modified gel for 48 hours. Once immobilized, no spontaneous detachment of the immobilized liposomes was observed. The degree of immobilization depended on both the thiol content and the ratio of the liposomes to the gel partilces. In a typical immobilization with 25 mol% 1-octanethiol, 82% of the liposomal phosphatidylcholine in the system was found to be associated with the gel. By decreasing the ratio of the liposomes to gel it was possible to bring the immobilization close to quantitative one. Among the three different thiols examined (1-octanethiol, 1-hexadecanethiol and thiocholesterol), the extent of the immobilization was slightly higher with thiocholesterol than the alkanethiols. The immobilized liposomes were detached from the gel with dithiothreitol. Approximately 60% of the fluorescent dextran derivative encapsulated in the liposomes was retained throughout the immobilization-detachment process. The gel left after the detachment remained active for immobilizing a fresh batch of thiol-liposomes.

Research paper thumbnail of Paper3

Immobilization of liposomes on hydrophobized Sephacryl gel and controlled detachment of the lipos... more Immobilization of liposomes on hydrophobized Sephacryl gel and controlled detachment of the liposomes from the gel were examined. The gel was chemically modified and bore octyl, hexadecyl or cholesteryl moiety via disulfide linkage as anchors to liposomal bilayer membrane. Upon interaction with the gel, egg phosphatidylcholine liposomes were successfully immobilized onto the gel. The gel with cholesteryl moiety showed 1.7 times higher liposome immobilization per anchor moiety than the gels with the alkyl moieties. The immobilization of liposomes on the gel was stable, and no significant spontaneous detachment of phospholipid or leakage of fluorescein isothiocyanate-conjugated dextran encapsulated in the immobilized liposomes was observed in 24 h. Reductive cleavage of the disulfide linkage by dithiothreitol resulted in detachment of the liposomes from the gel. The majority of the detached liposomes were found encapsulating the dextran derivative, and these liposomes should have kept their structural integrity throughout the immobilization and the detachment processes. The release of the liposomes was insignificant until the ratio of the dithiothreitol to the hydrophobic anchor reached a threshold. The presence of the threshold suggests that the immobilization of liposomes should require a certain minimum number of the hydrophobic moieties anchored in the liposomal membrane. By applying the present immobilization-detachment system, preparation of liposomes encapsulating the dextran derivative without using costly gel filtration or ultracentrifugation procedure was successfully demonstrated.

Research paper thumbnail of Paper 2

In situ formation of disulfide linkages between liposomes and Sephacryl gel particles both of tha... more In situ formation of disulfide linkages between liposomes and Sephacryl gel particles both of that were bearing mercapto moieties was demonstrated. The reaction led to immobilization of the liposomes on the gel. The immobilized liposomes could be detached by reductive treatment of the liposome-gel conjugate, and the resulting gel was still capable of further immobilizing liposomes.

Research paper thumbnail of Liposome Immobilization on Cross-linked Polymer Gel by In Situ Formation of Cleavable Covalent Bonds

Journal of Bioactive and Compatible Polymers, Nov 1, 2006

Research paper thumbnail of Trace metals contamination potential and health risk assessment of commonly consumed fish of Perak River, Malaysia

Research paper thumbnail of The Application of Nanotechnology in Medical Sciences: New Horizon of Treatment

American Journal of Biomedical Sciences, 2017

Nanotechnology has become enormously promising technology during last few decades and now it is e... more Nanotechnology has become enormously promising technology during last few decades and now it is estimated to have a significant effect on medical equipment. The possible effects of novel nanomedical applications on sickness identification, treatment, as well as anticipation are expected to improve healthiness care greatly. In addition, therapeutic selection may gradually be adapted to all patients' profile. Applications of nanomaterials are reported in operation, cancer diagnosis as well as therapy, biodetection of sickness markers, molecular imaging, implant technology, tissue engineering, as well as devices for drug, protein, and gene and radionuclide release. Various medical nanotechnology applications are at a standstill in their immaturity. A larger amount of nanodevices are presently under medical examination as well as several devices are commercially available. In this review work some applications of nanodevices in medical technology are also summarized.

Research paper thumbnail of Waterlogging in the Southwest Coastal Areas of Bangladesh: Local Adaptation Techniques and Challenges

American Scientific Research Journal for Engineering, Technology, and Sciences, 2020

Waterlogging is a critical environmental problem in the southwest coast of Bangladesh predominant... more Waterlogging is a critical environmental problem in the southwest coast of Bangladesh predominantly in some parts of Jashore, Satkhira and Khulna districts which create substantial impacts on livelihood. Therefore, this research aims to analyze how the coastal society deals with the increasing pressure on livelihood that emerged during and after waterlogging episodes. This research is dependent on empirically gathered data using both quantitative and qualitative approaches. A total 210 in-depth interviews from six unions, two unions from each of the three districts were conducted using a semi-structured questionnaire. In addition, six focus group discussion sessions and six key informant interviews were organized. For statistical analysis of collected data, SPSS was used. The study identifies some critical impacts of waterlogging on livelihood which include physical and mental illness, crop failure and damage, disruption in cropping mechanism, loss of income, and increasing level of...

Research paper thumbnail of Chikungunya outbreak (2017) in Bangladesh: Clinical profile, economic impact and quality of life during the acute phase of the disease

PLoS neglected tropical diseases, Jun 1, 2018

Chikungunya virus causes mosquito-transmitted infection that leads to extensive morbidity affecti... more Chikungunya virus causes mosquito-transmitted infection that leads to extensive morbidity affecting substantial quality of life. Disease associated morbidity, quality of life, and financial loss are seldom reported in resources limited countries, such as Bangladesh. We reported the acute clinical profile, quality of life and consequent economic burden of the affected individuals in the recent chikungunya outbreak (May to September 2017) in Dhaka city, Bangladesh. We conducted a cross-sectional study during the peak of chikungunya outbreak (July 24 to August 5, 2017) to document the clinical profiles of confirmed cases (laboratory test positive) and probable cases diagnosed by medical practitioners. Data related to clinical symptoms, treatment cost, loss of productivity due to missing work days, and quality of life during their first two-weeks of symptom onset were collected via face to face interview using a structured questionnaire. World Health Organization endorsed questionnaire ...

Research paper thumbnail of A Study Of Environmental Impacts On The Coral Resources In The Vicinity Of The Saint Martin Island, Bangladesh

International Journal of Scientific & Technology Research, Jan 25, 2016

A study of the environmental impacts on the coral resources in the vicinity of the Saint Martin I... more A study of the environmental impacts on the coral resources in the vicinity of the Saint Martin Island, Bangladesh was conducted with a view to making an assessment of the current status of coral resources in the island and identifying major natural and anthropogenic environmental threats to the future sustainability of these resources. It is evident that the coral resources have been reduced significantly, and currently, only 41 coral species are available. The existing environmental condition (assessed by pH, salinity, turbidity and temperature) in the island is not found responsible for the survival of the corals. The study also reveals that the major anthropogenic interventions are responsible for the gradual depletion of the coral resources. The major anthropogenic threats to the coral resources are coral collection and overfishing. In addition, coral extraction is identified as a potential threat to the future integrity of coral communities in the island. Environmental threats from anthropogenic activities related to sedimentation, land erosion and pollution are also the concerns for the coral communities in the island. For the conservation of the coral population, no coral monitoring cell has been established in this island.

Research paper thumbnail of Sludge Management in the Textile Industries of Bangladesh: An Industrial Survey of the Impact of the 2015 Standards and Guidelines

Water

The textile sector of Bangladesh has positively contributed to a significant impact on its nation... more The textile sector of Bangladesh has positively contributed to a significant impact on its national economy and employment opportunities due to its rapid growth. The increasing number of wet processing units has led to a growing amount of wastewater volume as well as textile sludge (a byproduct of wastewater or effluent treatment plants). In 2015, the government of Bangladesh instituted the “Bangladesh Standards and Guidelines for Sludge Management”. Therefore, this case study aimed to assess these standards’ impact on the textile industry’s sludge management practices, informing academic scholars of the research opportunities available, and serving as a policymaking tool for various other South Asia and Southeast Asia economies. The sludge management situation of thirty-six industries (namely, twelve dyeing, twelve printing, and twelve washing) was herein assessed through a self-administered questionnaire survey of respondents from the respective ‘Top Management’ and ‘Environmental...

Research paper thumbnail of Removing Reactive Dyes from Textile Effluent Using Banana Fibre

The ability of a particular banana fibre in removing reactive dyes from textile wastewater was st... more The ability of a particular banana fibre in removing reactive dyes from textile wastewater was studied in the present work. Locally available ground seeded banana fibre of ≤ 212 μ was used as adsorbent. Initially the adsorbent was tested on standard Novacron Blue FN-R reactive dye solutions and then it was also applied to raw textile wastewater. The adsorbent was found very effective to remove reactive dyes from water. The adsorption was found dependent on pH, contact time, adsorbent/dye ratio and initial concentration of the dye solution. It was found that the adsorption efficiency became maximum at pH 2 and it decreased with increasing pH. The adsorption was quite fast; approximately 90% of removal efficiency was achieved in 5 m, and the adsorption was completed in 20 m under suitable conditions. It was observed that at adsorbent-dye ratio of 333:1, the adsorbent was able to eliminate the reactive dye completely from standard aqueous solution within 20 m at pH 2. The removal effic...

Research paper thumbnail of Liposome immobilization on polymer gel particles by in situ formation of covalent linkages

Research paper thumbnail of Liposome immobilization on cross-linked polymer gel by in situ formation of cleavable covalent bonds

Journal of bioactive and …, 2006

Research paper thumbnail of HOGLA LEAF AS A POTENTIAL BIO-ADSORBENT FOR THE TREATMENT OF REACTIVE DYES IN TEXTILE EFFLUENTS

A new bio-adsorbent to remove reactive dyes from textile effluent was investigated in the present... more A new bio-adsorbent to remove reactive dyes from textile effluent was investigated in the present study. The adsorbent was the leaves of locally available hogla plant (Typha angustata). Initially, sunfix yellow, a reactive dye widely used in textile effluents, was used to check the removal efficiency in terms of contact time, pH of dye solution and adsorbent dosage. Complete removal (100%) of dye was achieved at adsorbent/dye ratio of 2300:1 at pH 10 with 180 minutes contact time. The adsorbent was then applied to deep colored, raw textile wastewater samples and it was found that 2.3 g of adsorbent was able to convert 100 mL of deep colored wastewater to transparent water at pH 10. Additionally, treatment by the adsorbent resulted in significant decreases in pH, BOD, COD, TS, TDS and TSS of wastewater, while improving the DO level.

Research paper thumbnail of An Investigation Into The Water Quality Of Buriganga -A River Running Through Dhaka

Buriganga river is used for bathing, drinking, irrigation and industrial purposes and is consider... more Buriganga river is used for bathing, drinking, irrigation and industrial purposes and is considered to be the lifeline of Dhaka city. The water quality of Buriganga has become a matter of concern due to serious levels of pollution. The objective of the study was to determine the water quality of the selected section of Buriganga river which passes through Dhaka city. The water quality parameters were sampled during different seasons (summer, winter and autumn) and in 10 different sampling points along the river along the banks of the Buriganga River. The water quality parameters studied for this study were dissolved oxygen (DO), biochemical oxygen demand (BOD), chemical oxygen demand (COD), pH, turbidity, conductivity, total dissolved solids (TDS), nitrate and phosphate. The results showed that DO, BOD, COD, TDS, turbidity, nitrate and phosphate are at an alarming level and a discussion on the possible sources of the pollution are presented.

Research paper thumbnail of JNN

Fabrication of a hybrid consists of gold nanoparticles and multi walled carbon nanotubes (MWCNTs)... more Fabrication of a hybrid consists of gold nanoparticles and multi walled carbon nanotubes (MWCNTs) with the help of poly (amino acid) was investigated. Poly(acryloyl -alanine) was synthesized by precipitation polymerization in tetrahydrofuran. The polymers were used to form hybrids with MWCNTs in aqueous media. Subsequently, the polymer functionalized MWCNTs were fabricated by in situ formed gold nanoparticles. The fabrication by gold nanoparticles was confirmed by transmission electron microscopic analyses. The fabrication was attempted with different concentrations of lithium auric chloride solutions in the range of 0.1-1.2 mM in water. The lower concentration of the gold precursor solution resulted in the formation and attachment of gold nanoparticles without aggregation while the higher concentration above 1.0 mM led to the aggregation of gold nanoparticles. The gold nanoparticles were observed only on the surface of MWCNTs and none was in the bulk aqueous phase.

Research paper thumbnail of 2016-CEJ-Online

Research paper thumbnail of CEJ-2014

Research paper thumbnail of EPDM Rubber

Conducting rubber composites of ethylene propylene diene M-class rubber (EPDM) were prepared with... more Conducting rubber composites of ethylene propylene diene M-class rubber (EPDM) were prepared with multi walled carbon nanotubes (MWCNTs) and organo-clay, Cloisite Ò 15A to develop flexible strain sensitive materials. The composites were prepared with a constant amount of organo-clay and various amounts of MWCNTs from 5 to 50 wt.%. Organo-clay led to improved dispersion of MWCNTs in the rubber matrix. Upon increasing the MWCNT contents, the tensile strength, stiffness and electrical conductivity of the composites increased significantly but the elongation at break decreased in the EPDM/MWCNT composites. The organo-clay was able to improve tensile strength, stiffness, elongation at break and electrical conductivity of the composites. The non-symmetric linear resistance change was observed by the composites under deformation.

Research paper thumbnail of Paper 4

Immobilization of liposomes onto chemically modified Sephacryl gel particles by in situ reaction ... more Immobilization of liposomes onto chemically modified Sephacryl gel particles by in situ reaction between liposome-incorporated thiols and mercapto moieties on the gel to form disulfide linkages was investigated. For the immobilization, both the mercapto moieties and the incorporated thiol were essential. The immobilization occurred upon coincubation of the modified liposomes with the modified gel for 48 hours. Once immobilized, no spontaneous detachment of the immobilized liposomes was observed. The degree of immobilization depended on both the thiol content and the ratio of the liposomes to the gel partilces. In a typical immobilization with 25 mol% 1-octanethiol, 82% of the liposomal phosphatidylcholine in the system was found to be associated with the gel. By decreasing the ratio of the liposomes to gel it was possible to bring the immobilization close to quantitative one. Among the three different thiols examined (1-octanethiol, 1-hexadecanethiol and thiocholesterol), the extent of the immobilization was slightly higher with thiocholesterol than the alkanethiols. The immobilized liposomes were detached from the gel with dithiothreitol. Approximately 60% of the fluorescent dextran derivative encapsulated in the liposomes was retained throughout the immobilization-detachment process. The gel left after the detachment remained active for immobilizing a fresh batch of thiol-liposomes.

Research paper thumbnail of Paper 4

Immobilization of liposomes onto chemically modified Sephacryl gel particles by in situ reaction ... more Immobilization of liposomes onto chemically modified Sephacryl gel particles by in situ reaction between liposome-incorporated thiols and mercapto moieties on the gel to form disulfide linkages was investigated. For the immobilization, both the mercapto moieties and the incorporated thiol were essential. The immobilization occurred upon coincubation of the modified liposomes with the modified gel for 48 hours. Once immobilized, no spontaneous detachment of the immobilized liposomes was observed. The degree of immobilization depended on both the thiol content and the ratio of the liposomes to the gel partilces. In a typical immobilization with 25 mol% 1-octanethiol, 82% of the liposomal phosphatidylcholine in the system was found to be associated with the gel. By decreasing the ratio of the liposomes to gel it was possible to bring the immobilization close to quantitative one. Among the three different thiols examined (1-octanethiol, 1-hexadecanethiol and thiocholesterol), the extent of the immobilization was slightly higher with thiocholesterol than the alkanethiols. The immobilized liposomes were detached from the gel with dithiothreitol. Approximately 60% of the fluorescent dextran derivative encapsulated in the liposomes was retained throughout the immobilization-detachment process. The gel left after the detachment remained active for immobilizing a fresh batch of thiol-liposomes.

Research paper thumbnail of Paper3

Immobilization of liposomes on hydrophobized Sephacryl gel and controlled detachment of the lipos... more Immobilization of liposomes on hydrophobized Sephacryl gel and controlled detachment of the liposomes from the gel were examined. The gel was chemically modified and bore octyl, hexadecyl or cholesteryl moiety via disulfide linkage as anchors to liposomal bilayer membrane. Upon interaction with the gel, egg phosphatidylcholine liposomes were successfully immobilized onto the gel. The gel with cholesteryl moiety showed 1.7 times higher liposome immobilization per anchor moiety than the gels with the alkyl moieties. The immobilization of liposomes on the gel was stable, and no significant spontaneous detachment of phospholipid or leakage of fluorescein isothiocyanate-conjugated dextran encapsulated in the immobilized liposomes was observed in 24 h. Reductive cleavage of the disulfide linkage by dithiothreitol resulted in detachment of the liposomes from the gel. The majority of the detached liposomes were found encapsulating the dextran derivative, and these liposomes should have kept their structural integrity throughout the immobilization and the detachment processes. The release of the liposomes was insignificant until the ratio of the dithiothreitol to the hydrophobic anchor reached a threshold. The presence of the threshold suggests that the immobilization of liposomes should require a certain minimum number of the hydrophobic moieties anchored in the liposomal membrane. By applying the present immobilization-detachment system, preparation of liposomes encapsulating the dextran derivative without using costly gel filtration or ultracentrifugation procedure was successfully demonstrated.

Research paper thumbnail of Paper 2

In situ formation of disulfide linkages between liposomes and Sephacryl gel particles both of tha... more In situ formation of disulfide linkages between liposomes and Sephacryl gel particles both of that were bearing mercapto moieties was demonstrated. The reaction led to immobilization of the liposomes on the gel. The immobilized liposomes could be detached by reductive treatment of the liposome-gel conjugate, and the resulting gel was still capable of further immobilizing liposomes.