Kristen Vanderveen - Academia.edu (original) (raw)

Uploads

Papers by Kristen Vanderveen

Research paper thumbnail of Synthesis of a Methylenebis(phosphonate) Analogue of Mycophenolic Adenine Dinucleotide: A Glucuronidation-Resistant MAD Analogue of NAD

Journal of Medicinal Chemistry, Jan 24, 1998

Research paper thumbnail of Ternary Complex of Human Type-II Inosine Monophosphate Dehydrogenase with 6-CL-IMP and Selenazole Adenine Dinucleotide

Research paper thumbnail of Synthesis of Nonhydrolyzable Analogues of Thiazole-4-carboxamide and Benzamide Adenine Dinucleotide Containing Fluorine Atom at the C2‘ of Adenine Nucleoside: Induction of K562 Differentiation and Inosine Monophosphate Dehydrogenase Inhibitory Activity

Journal of Medicinal Chemistry, Aug 1, 1997

Research paper thumbnail of Crystal structure of human type II inosine monophosphate dehydrogenase: Implications for ligand binding and drug design

Proceedings of the National Academy of Sciences of the United States of America, Mar 30, 1999

Inosine monophosphate dehydrogenase (IM-PDH) controls a key metabolic step in the regulation of c... more Inosine monophosphate dehydrogenase (IM-PDH) controls a key metabolic step in the regulation of cell growth and differentiation. This step is the NAD-dependent oxidation of inosine 5 monophosphate (IMP) to xanthosine 5 monophosphate, the rate-limiting step in the synthesis of the guanine nucleotides. Two isoforms of IMPDH have been identified, one of which (type II) is significantly up-regulated in neoplastic and differentiating cells. As such, it has been identified as a major target in antitumor and immunosuppressive drug design. We present here the 2.9-Å structure of a ternary complex of the human type II isoform of IMPDH. The complex contains the substrate analogue 6-chloropurine riboside 5-monophosphate (6-Cl-IMP) and the NAD analogue selenazole-4carboxamide adenine dinucleotide, the selenium derivative of the active metabolite of the antitumor drug tiazofurin. The enzyme forms a homotetramer, with the dinucleotide binding at the monomer-monomer interface. The 6 chloro-substituted purine base is dehalogenated, forming a covalent adduct at C6 with Cys-331. The dinucleotide selenazole base is stacked against the 6-Cl-IMP purine ring in an orientation consistent with the B-side stereochemistry of hydride transfer seen with NAD. The adenosine end of the ligand interacts with residues not conserved between the type I and type II isoforms, suggesting strategies for the design of isoform-specific agents.

Research paper thumbnail of Ternary Complex of Human Type-II Inosine Monophosphate Dehydrogenase with 6-CL-IMP and Selenazole Adenine Dinucleotide

Research paper thumbnail of Synthesis of Nonhydrolyzable Analogues of Thiazole-4-carboxamide and Benzamide Adenine Dinucleotide Containing Fluorine Atom at the C2‘ of Adenine Nucleoside: Induction of K562 Differentiation and Inosine Monophosphate Dehydrogenase Inhibitory Activity

Journal of Medicinal Chemistry, 1997

Research paper thumbnail of Synthesis of a Methylenebis(phosphonate) Analogue of Mycophenolic Adenine Dinucleotide:  A Glucuronidation-Resistant MAD Analogue of NAD

Journal of Medicinal Chemistry, 1998

Research paper thumbnail of Crystal structure of human type II inosine monophosphate dehydrogenase: Implications for ligand binding and drug design

Proceedings of the National Academy of Sciences, 1999

Inosine monophosphate dehydrogenase (IMPDH) controls a key metabolic step in the regulation of ce... more Inosine monophosphate dehydrogenase (IMPDH) controls a key metabolic step in the regulation of cell growth and differentiation. This step is the NAD-dependent oxidation of inosine 5′ monophosphate (IMP) to xanthosine 5′ monophosphate, the rate-limiting step in the synthesis of the guanine nucleotides. Two isoforms of IMPDH have been identified, one of which (type II) is significantly up- regulated in neoplastic and differentiating cells. As such, it has been identified as a major target in antitumor and immunosuppressive drug design. We present here the 2.9-Å structure of a ternary complex of the human type II isoform of IMPDH. The complex contains the substrate analogue 6-chloropurine riboside 5′-monophosphate (6-Cl-IMP) and the NAD analogue selenazole-4-carboxamide adenine dinucleotide, the selenium derivative of the active metabolite of the antitumor drug tiazofurin. The enzyme forms a homotetramer, with the dinucleotide binding at the monomer–monomer interface. The 6 chloro-subs...

Research paper thumbnail of Synthesis of a Methylenebis(phosphonate) Analogue of Mycophenolic Adenine Dinucleotide: A Glucuronidation-Resistant MAD Analogue of NAD

Journal of Medicinal Chemistry, Jan 24, 1998

Research paper thumbnail of Ternary Complex of Human Type-II Inosine Monophosphate Dehydrogenase with 6-CL-IMP and Selenazole Adenine Dinucleotide

Research paper thumbnail of Synthesis of Nonhydrolyzable Analogues of Thiazole-4-carboxamide and Benzamide Adenine Dinucleotide Containing Fluorine Atom at the C2‘ of Adenine Nucleoside: Induction of K562 Differentiation and Inosine Monophosphate Dehydrogenase Inhibitory Activity

Journal of Medicinal Chemistry, Aug 1, 1997

Research paper thumbnail of Crystal structure of human type II inosine monophosphate dehydrogenase: Implications for ligand binding and drug design

Proceedings of the National Academy of Sciences of the United States of America, Mar 30, 1999

Inosine monophosphate dehydrogenase (IM-PDH) controls a key metabolic step in the regulation of c... more Inosine monophosphate dehydrogenase (IM-PDH) controls a key metabolic step in the regulation of cell growth and differentiation. This step is the NAD-dependent oxidation of inosine 5 monophosphate (IMP) to xanthosine 5 monophosphate, the rate-limiting step in the synthesis of the guanine nucleotides. Two isoforms of IMPDH have been identified, one of which (type II) is significantly up-regulated in neoplastic and differentiating cells. As such, it has been identified as a major target in antitumor and immunosuppressive drug design. We present here the 2.9-Å structure of a ternary complex of the human type II isoform of IMPDH. The complex contains the substrate analogue 6-chloropurine riboside 5-monophosphate (6-Cl-IMP) and the NAD analogue selenazole-4carboxamide adenine dinucleotide, the selenium derivative of the active metabolite of the antitumor drug tiazofurin. The enzyme forms a homotetramer, with the dinucleotide binding at the monomer-monomer interface. The 6 chloro-substituted purine base is dehalogenated, forming a covalent adduct at C6 with Cys-331. The dinucleotide selenazole base is stacked against the 6-Cl-IMP purine ring in an orientation consistent with the B-side stereochemistry of hydride transfer seen with NAD. The adenosine end of the ligand interacts with residues not conserved between the type I and type II isoforms, suggesting strategies for the design of isoform-specific agents.

Research paper thumbnail of Ternary Complex of Human Type-II Inosine Monophosphate Dehydrogenase with 6-CL-IMP and Selenazole Adenine Dinucleotide

Research paper thumbnail of Synthesis of Nonhydrolyzable Analogues of Thiazole-4-carboxamide and Benzamide Adenine Dinucleotide Containing Fluorine Atom at the C2‘ of Adenine Nucleoside: Induction of K562 Differentiation and Inosine Monophosphate Dehydrogenase Inhibitory Activity

Journal of Medicinal Chemistry, 1997

Research paper thumbnail of Synthesis of a Methylenebis(phosphonate) Analogue of Mycophenolic Adenine Dinucleotide:  A Glucuronidation-Resistant MAD Analogue of NAD

Journal of Medicinal Chemistry, 1998

Research paper thumbnail of Crystal structure of human type II inosine monophosphate dehydrogenase: Implications for ligand binding and drug design

Proceedings of the National Academy of Sciences, 1999

Inosine monophosphate dehydrogenase (IMPDH) controls a key metabolic step in the regulation of ce... more Inosine monophosphate dehydrogenase (IMPDH) controls a key metabolic step in the regulation of cell growth and differentiation. This step is the NAD-dependent oxidation of inosine 5′ monophosphate (IMP) to xanthosine 5′ monophosphate, the rate-limiting step in the synthesis of the guanine nucleotides. Two isoforms of IMPDH have been identified, one of which (type II) is significantly up- regulated in neoplastic and differentiating cells. As such, it has been identified as a major target in antitumor and immunosuppressive drug design. We present here the 2.9-Å structure of a ternary complex of the human type II isoform of IMPDH. The complex contains the substrate analogue 6-chloropurine riboside 5′-monophosphate (6-Cl-IMP) and the NAD analogue selenazole-4-carboxamide adenine dinucleotide, the selenium derivative of the active metabolite of the antitumor drug tiazofurin. The enzyme forms a homotetramer, with the dinucleotide binding at the monomer–monomer interface. The 6 chloro-subs...

Log In