Kundan Prasad - Academia.edu (original) (raw)

Papers by Kundan Prasad

World Journal of Pharmaceutical Research, 2015

Wild plants play a vital role in the health security of Himalayan inhabitants and constitute sign... more Wild plants play a vital role in the health security of Himalayan inhabitants and constitute significantly to their diet. In this report, we investigated the nutritional composition of Euphorbia hirta Linn growing at high altitudes in the Himalayas. The collected plant materials were first washed with cold water to remove the soil particles and then shade dried. The dried material was finely powdered in the grinding machine and weighed in an electrical balance. The dried material was finely powdered in the grinding machine and weighed in an electrical balance. Amino acid analysis was performed using the Waters Associates PICO-TAG method. The total amino acid content in P. indica was 58.80 mg amino acid/g sample (dry weight), E. thymifolia was 123.92 mg amino acid/g sample (dry weight) and P. hirta was 225.73 mg amino acid/g sample (dry weight). The total essential amino acids in P. indica, E. thymifolia and P. hirta were 33.58, 57.99 and 145.82 mg amino acid/g respectively. Among th...

Journal of pharmacology & clinical research, 2017

200 mg/kg) showed antiurolithiatic potential against calcium oxalate stones when tested against e... more 200 mg/kg) showed antiurolithiatic potential against calcium oxalate stones when tested against ethylene glycol induced

This study investigates the influence of extraction system on the extractability of phytochemical... more This study investigates the influence of extraction system on the extractability of phytochemical compounds and antioxidant activity of ethanolic extracts of Viola serpens & Morus nigra. It can be concluded that the solvent used affects significantly the phytochemical content and the antioxidant activity of the extract and therefore it is recommended to use more than one extraction system for better assessment of the antioxidant activity of natural products. Several of the investigated herbs contain substantial amounts of free radical scavengers and can serve as a potential source of natural antioxidants for medicinal and commercial uses. The phytochemical screening of ethanolic extract of M.nigra showed the the presence of tannins and terpenoids where as alkaloids, saponins, flavonoids, amino acids, reducing sugars and glycosides were absent. Ethanolic extract of V.serpens showed the absence of alkaloids, saponins, tannins, terpenoids and glycosides, flavonoids amino acids and redu...

Vegetable Science, 2006

A field experiment was laid out with tomato cv. Kashi Anupam at the research farm of Indian Insti... more A field experiment was laid out with tomato cv. Kashi Anupam at the research farm of Indian Institute of Vegetable Research, Varanasi during 2002-2003. The treatments consisted of endosulfan 35 EC @ single dose 2.5 mill it, double dose 5.0 mlJlit and an untreated control. Fruit samples were collected from each replicate at 0, 3, 5, 7, 15 and 30 days after second spray and the residue levels were analyzed. From composite fruit sample (500g), a portion of sample (50 g) was blended in a high-speed blender and extracted With acetone and isopropanol (250) in 3:1. The suspension was filtered under slight vacuum through Buchner funnel overlaid with Wattman No.­ I filter paper. The aqueous phase was partitioned with n-hexane (3 x 50 ml). Aqueous phase was discarded and hexane phase was concentrated to 50 ml in a RotoryVacuum Evaporator. To this 50 ml concentrate, activated charcoal 10.5g) was added for adsorbing the pigments. Later this mixture was filtered and concentrated to dryness. The ...

Plant essential oils are potential source of antimicrobials of natural origin. Essential oils and... more Plant essential oils are potential source of antimicrobials of natural origin. Essential oils and extracts obtained from many plants have recently gained a great popularity and scientific interest. Consumer demand for natural preservatives has increased, whereas the safety aspect of chemical additives has been questioned. The plant oil has been reported to have antibacterial, antifungal, antiviral, antiparasitic and antidermatophytic properties. Thus, this study aimed to investigate the antifungal activity of three essential oils (EOs), against Picher guilliermondii and Candida albicans strains. Three medicinally important plants Swertia ciliata, Acorus calamus and Viola serpens were evaluated for their antifungal activities. The oil of Swertia ciliata had moderate to good activity against the tested Candida albicans pathogens and the oil of Acorus calamus and Viola serpens show good activity against Pichia guilliermondii on the basis of zone of inhibition and MIC values. These resu...

Sweet flag (Acorus calamus) is mentioned in Ayurveda and belongs to the genus Acorus L. of the fa... more Sweet flag (Acorus calamus) is mentioned in Ayurveda and belongs to the genus Acorus L. of the family Acoraceae and is widely distributed temperate to sub temperate regions. It is commonly used in traditional medicinal systems of Asian and European countries to treat appetite loss, diarrhoea, digestive disorders, bronchitis indigestion, chest pain, nervous disorders. This review focuses on the therapeutic potential of essential oils, phenyl propanoids, sesquiterpenes and monoterpenes as well as xanthone, glycosides, flavones, lignans, steroids and inorganic constituents.

Introduction: Euphorbia pilosa is much branch, milky latex bearing tall herb commonly used to tre... more Introduction: Euphorbia pilosa is much branch, milky latex bearing tall herb commonly used to treat bronchitis, Purgative, emetic, fistulous sores and asthma-related problems. The plants were collected in the month of September 2015 from Kalamuni (Munsiari) near Pithoragarh, location of Kumaon Himalayas. Methods: The plant Euphorbia pilosa including leaves, stem, and flowers were extracted by hydro distillation method for 6 hours using Clevenger apparatus. The hydro-distilled essential oil of Euphorbia pilosa has been examined by means of gas chromatography-mass spectrometry (GC-MS). Results: Phytochemical investigation of whole plants of Euphorbia pilosa led to the isolation and identification of essential oil. Separation was achieved by Elite-5MS fused capillary column. The mass spectra were compared with the spectra of known components stored in the NIST and WILEY databases for compound identification. Forty-six chemical constituents were identified. The major constituents were Phytol (5.75%), n-Pentadecanal (5.12%), n-Pentadecane (4.02 %), δ-Terpineol (3.31) and Tricos-(9Z)-ene (3.24%). Conclusion: The results data obtained in the present study suggest that essential oil possesses strong medicinal activities can be utilized for treatment of diseases.

Journal of pharmacology & clinical research, Oct 25, 2017

Medicinal plants play a pivotal role in the health care of ancient and modern cultures. The plant... more Medicinal plants play a pivotal role in the health care of ancient and modern cultures. The plant Medicinal plants play a pivotal role in the health care of ancient and modern cultures. The plant Leptodermis lanceolata including leaves, stem flower, were extracted by hydro distillation method for 6 hours using Clevenger apparatus The hydro-distilled essential oil of Leptodermis lanceolata has been examined by means of gas chromatography-mass spectrometry (GC-MS). Take 1.0 ml E. Oil of various code samples and mixed with in 4.0 ml DMSO (Dimethyl sulfoxide). The prepared extract was used for the determination of antioxidant activity (i.e., DPPH assay & ABTS assay) in among samples. The hydro-distilled essential oil of Leptodermis lanceolata has been examined by means of gas chromatography-mass spectrometry (GC-MS).Thirty-four constituents have been identified representing 72.29% of the total oil. The main compounds were phytol 19.44%, Linalool 12.12%, n-Hexadecanoic acid 7.55%, 4(E)-4-Hexenyl acetate 4.49%, the compound in minor amount were γ-Cadinene 0.21%, Neryl acetone 0.32%, 4,6-Dimethyl dodecane 0.32%, The oxygenated diterpene (36.68%), and oxygenated monoterpene (20%), found in the oil as major components while sesquterpenes (5.72%),were minor components. The results data obtained in the present study suggest that some essential oils possess strong medicinal activities, which can be utilized for treatment of certain diseases.

Revista Brasileira de Saúde e Produção Animal, 2018

SUMMARY Resistance to use antifungal drugs is a great concern seeking for scientists to discover ... more SUMMARY Resistance to use antifungal drugs is a great concern seeking for scientists to discover new products to treat fungal infections. The aim of this study was to evaluate the antioxidant and antifungal activities of essential oils and extracts of Plectranthus grandis and Plectranthus ornatus against Trichophyton rubrum and Microsporum canis dermatophytes strains. Extracts were obtained from leaves by maceration in ethanol (96%) during 7 days. The oils were obtained by hydrodistillation and analyzed by gas chromatography/mass spectrometry. A total of 25 components were identified, as major constituents the sesquiterpenes β-caryophyllene, α-copaene, germacrene, β-caryophyllene and caryophyllene oxide. Antioxidant activities were evaluated using DPPH scavenging assay and antifungal action was determined by the broth microdilution method. The decocts obtained from the extraction of essential oil presented a greater antioxidant action when compared with the essential oils, with IC50...

Journal of Analytical & Pharmaceutical Research, 2017

Antioxidant phytochemicals such as vitamin C, β-carotene, lutein, α-tocopherol, amino acid and to... more Antioxidant phytochemicals such as vitamin C, β-carotene, lutein, α-tocopherol, amino acid and total phenolics, were estimated in some medicinal plants using a reverse phase HPLC system. Wild plants play a vital role in the health security of Himalayan inhabitants and constitute significantly to their diet. Amino acid was analysis performed using the Waters Associates PICO-TAG method. The total amino acid content in P. indica was 58.80mg amino acid/g sample (dry weight), E. thymifolia was 123.92mg amino acid/g sample (dry weight) and P. hirta was 225.73mg amino acid/g sample (dry weight). The total essential amino acids in P. indica, E. thymifolia and P. hirta were 33.58, 57.99 and 145.82mg amino acid/g respectively. Among the three investigated plants, Carotenoids viz xanthophylls content was found 0.13 to 151.01 mg/100g dry weight basis. The maximum xanthophylls content was found in P. indica leaves and minimum in P. hirta rhizomes. The β-carotene content varies from 4.62-374.55mg/100g on dry weight basis. DL-α-tocopherol in these medicinal plants was found 13.48mg/100g, 24.95mg/100g and 9.13mg/100g on dry weight basis in P. hirta, E. thymifolia and P. indica respectively. This study discovers amino acid and antioxidant content in medicinal plants. Such information will increase the understanding of the faction of these antioxidant phytochemical in lowering incidence of ageing and other chronic diseases. Results of our study suggest the great value of these species for use in pharmacy and phytotherapy. Based on this information, it could be concluded that this plant is natural sources of antioxidant substances of high importance.

Journal of Pharmaceutics & Pharmacology, 2018

Introduction: Polygonum amplexicaule (Polygonaceae) is an herbal drug used to treat fractures, rh... more Introduction: Polygonum amplexicaule (Polygonaceae) is an herbal drug used to treat fractures, rheumatism, osteoporosis, muscle injuries and pain. It has also been reported to be effective to treat atherosclerosis and its antibiotic and antivirus effects. Methods: The plant Polygonum amplexicaule including leaves, stem, and flowers were extracted by hydro distillation method for 6 hours using Clevenger apparatus. Results: Total fifty one compounds were identified constituting 96.09% of the total oil. The main compounds was Geraniol (19.91%), β-Linalool (19.63), Citronellol (16.22%), 2-Methyl-6-hepten-1-ol (7.77%), Heptan-2-ol (5.66). Conclusion: The results data obtained in the present study suggest that an essential oil and whole plant possesses strong medicinal activities can be utilized for treatment of diseases.

World Journal of Pharmaceutical Research, 2017

The hydro-distilled essential oil of Excocaria acerifolia has been examined by means of gas chrom... more The hydro-distilled essential oil of Excocaria acerifolia has been examined by means of gas chromatography-mass spectrometry (GCMS). The oil constituents were identified according to their mass spectra and their relative retention indices determined on a non-polar stationary phase capillary column. Fifty-seven constituents have been identified representing 93.37% of the total oil. The main compounds in major amounts were Isopropyl isothiocyanate (14.03%), (E)- Caryophyllen (11.65%), Benzyl isothiocyanate (9.32%), 1, 3, 8- paramenthatriene (6.61%), the compound in trace or in minor amount were n-hexanol (0.06%), 2-Ethyl-5-methyl tetrahydrofuran (0.06%), the oil was found rich in oxygenated mono and sesquiterpene hydrocarbons.

World Journal of Pharmaceutical Research, 2017

Research Journal of Phytochemistry, 2014

Research Journal of Phytochemistry, 2014

Biomedical Chromatography, 2009

The flavonoid content of several methanolic extract fractions of Navel orange peel (flavedo and a... more The flavonoid content of several methanolic extract fractions of Navel orange peel (flavedo and albedo of Citrus sinensis) cultivated in Crete (Greece) was first analysed phytochemically and then assessed for its antioxidant activity in vitro. The chemical structures of the constituents fractionated were originally determined by comparing their retention times and the obtained UV spectral data with the available bibliographic data and further verified by detailed LC-DAD-MS (ESI+) analysis. The main flavonoid groups found within the fractions examined were polymethoxylated flavones, O-glycosylated flavones, C-glycosylated flavones, O-glycosylated flavonols, O-glycosylated flavanones and phenolic acids along with their ester derivatives. In addition, the quantitative HPLC analysis confirmed that hesperidin is the major flavonoid glycoside found in the orange peel. Interestingly enough, its quantity at 48 mg/g of dry peel permits the commercial use of orange peel as a source for the production of hesperidin. The antioxidant activity of the orange peel methanolic extract fractions was evaluated by applying two complementary methodologies, DPPH • assay and the Co(II)/EDTA-induced luminol chemiluminescence approach. Overall, the results have shown that orange peel methanolic extracts possess moderate antioxidant activity as compared with the activity seen in tests where the corresponding aglycones, diosmetin and hesperetin were assessed in different ratios.

ethnoleaflets.com

... Corresponding author Arvind Bhatt, School of Biological & Conservation Sciences. Universi... more ... Corresponding author Arvind Bhatt, School of Biological & Conservation Sciences. University of Kwazulu-Natal , Private Bag X54001, Durban 4000, South Africa. ... Kala, CP . 2002. Medicinal Plants of Indian Trans-Himalaya Bishen Singh Mahendra Pal Singh, Dehradun , India ,. ...

Journal of Medicinal Plants Research, 2017

The families Violaceae (alternatively known as Alsodeiace or Leoniaceae or Retrosepalaceae) compr... more The families Violaceae (alternatively known as Alsodeiace or Leoniaceae or Retrosepalaceae) comprise twenty genera and about 800 species. Viola serpens belongs to family Violaceae and commonly known as "Banafsa". It is a small glabrous, perennial herb, which is found throughout India in moist woods and hilly districts. The essential oil of aerial parts of V. serpens, were extracted by steam distillation. The quantitative and qualitative analysis of volatile essential oil constituents of the plant was done by Gas Chromatography (GC) and GC-Mass Spectrometry. A total of 50 components of the essential oil of V. serpens were identified, accounting for 81.38% of the total oil. The main compounds found were Bis (2ethylhexyl) maleate (15.62%), 2, 4, 4, 6-Tetramethyl-2-heptene (11.52%), Hexen-3-ol (6.56%), and Cis Verbeno (l 4.77%). The chemical constituents in the essential oil from V. serpens were identified in the following classes or groups of chemical compounds, such as monoterpens, sesquiterpenes volatile organic compounds and their oxygenated hydrocarbons. Therefore, the essential constituents could be used as antioxidant, antifungal or antimicrobial agent in new drugs preparation for therapy of infectious diseases.

World Journal of Pharmaceutical Research, 2015

Wild plants play a vital role in the health security of Himalayan inhabitants and constitute sign... more Wild plants play a vital role in the health security of Himalayan inhabitants and constitute significantly to their diet. In this report, we investigated the nutritional composition of Euphorbia hirta Linn growing at high altitudes in the Himalayas. The collected plant materials were first washed with cold water to remove the soil particles and then shade dried. The dried material was finely powdered in the grinding machine and weighed in an electrical balance. The dried material was finely powdered in the grinding machine and weighed in an electrical balance. Amino acid analysis was performed using the Waters Associates PICO-TAG method. The total amino acid content in P. indica was 58.80 mg amino acid/g sample (dry weight), E. thymifolia was 123.92 mg amino acid/g sample (dry weight) and P. hirta was 225.73 mg amino acid/g sample (dry weight). The total essential amino acids in P. indica, E. thymifolia and P. hirta were 33.58, 57.99 and 145.82 mg amino acid/g respectively. Among th...

Journal of pharmacology & clinical research, 2017

200 mg/kg) showed antiurolithiatic potential against calcium oxalate stones when tested against e... more 200 mg/kg) showed antiurolithiatic potential against calcium oxalate stones when tested against ethylene glycol induced

This study investigates the influence of extraction system on the extractability of phytochemical... more This study investigates the influence of extraction system on the extractability of phytochemical compounds and antioxidant activity of ethanolic extracts of Viola serpens & Morus nigra. It can be concluded that the solvent used affects significantly the phytochemical content and the antioxidant activity of the extract and therefore it is recommended to use more than one extraction system for better assessment of the antioxidant activity of natural products. Several of the investigated herbs contain substantial amounts of free radical scavengers and can serve as a potential source of natural antioxidants for medicinal and commercial uses. The phytochemical screening of ethanolic extract of M.nigra showed the the presence of tannins and terpenoids where as alkaloids, saponins, flavonoids, amino acids, reducing sugars and glycosides were absent. Ethanolic extract of V.serpens showed the absence of alkaloids, saponins, tannins, terpenoids and glycosides, flavonoids amino acids and redu...

Vegetable Science, 2006

A field experiment was laid out with tomato cv. Kashi Anupam at the research farm of Indian Insti... more A field experiment was laid out with tomato cv. Kashi Anupam at the research farm of Indian Institute of Vegetable Research, Varanasi during 2002-2003. The treatments consisted of endosulfan 35 EC @ single dose 2.5 mill it, double dose 5.0 mlJlit and an untreated control. Fruit samples were collected from each replicate at 0, 3, 5, 7, 15 and 30 days after second spray and the residue levels were analyzed. From composite fruit sample (500g), a portion of sample (50 g) was blended in a high-speed blender and extracted With acetone and isopropanol (250) in 3:1. The suspension was filtered under slight vacuum through Buchner funnel overlaid with Wattman No.­ I filter paper. The aqueous phase was partitioned with n-hexane (3 x 50 ml). Aqueous phase was discarded and hexane phase was concentrated to 50 ml in a RotoryVacuum Evaporator. To this 50 ml concentrate, activated charcoal 10.5g) was added for adsorbing the pigments. Later this mixture was filtered and concentrated to dryness. The ...

Plant essential oils are potential source of antimicrobials of natural origin. Essential oils and... more Plant essential oils are potential source of antimicrobials of natural origin. Essential oils and extracts obtained from many plants have recently gained a great popularity and scientific interest. Consumer demand for natural preservatives has increased, whereas the safety aspect of chemical additives has been questioned. The plant oil has been reported to have antibacterial, antifungal, antiviral, antiparasitic and antidermatophytic properties. Thus, this study aimed to investigate the antifungal activity of three essential oils (EOs), against Picher guilliermondii and Candida albicans strains. Three medicinally important plants Swertia ciliata, Acorus calamus and Viola serpens were evaluated for their antifungal activities. The oil of Swertia ciliata had moderate to good activity against the tested Candida albicans pathogens and the oil of Acorus calamus and Viola serpens show good activity against Pichia guilliermondii on the basis of zone of inhibition and MIC values. These resu...

Sweet flag (Acorus calamus) is mentioned in Ayurveda and belongs to the genus Acorus L. of the fa... more Sweet flag (Acorus calamus) is mentioned in Ayurveda and belongs to the genus Acorus L. of the family Acoraceae and is widely distributed temperate to sub temperate regions. It is commonly used in traditional medicinal systems of Asian and European countries to treat appetite loss, diarrhoea, digestive disorders, bronchitis indigestion, chest pain, nervous disorders. This review focuses on the therapeutic potential of essential oils, phenyl propanoids, sesquiterpenes and monoterpenes as well as xanthone, glycosides, flavones, lignans, steroids and inorganic constituents.

Introduction: Euphorbia pilosa is much branch, milky latex bearing tall herb commonly used to tre... more Introduction: Euphorbia pilosa is much branch, milky latex bearing tall herb commonly used to treat bronchitis, Purgative, emetic, fistulous sores and asthma-related problems. The plants were collected in the month of September 2015 from Kalamuni (Munsiari) near Pithoragarh, location of Kumaon Himalayas. Methods: The plant Euphorbia pilosa including leaves, stem, and flowers were extracted by hydro distillation method for 6 hours using Clevenger apparatus. The hydro-distilled essential oil of Euphorbia pilosa has been examined by means of gas chromatography-mass spectrometry (GC-MS). Results: Phytochemical investigation of whole plants of Euphorbia pilosa led to the isolation and identification of essential oil. Separation was achieved by Elite-5MS fused capillary column. The mass spectra were compared with the spectra of known components stored in the NIST and WILEY databases for compound identification. Forty-six chemical constituents were identified. The major constituents were Phytol (5.75%), n-Pentadecanal (5.12%), n-Pentadecane (4.02 %), δ-Terpineol (3.31) and Tricos-(9Z)-ene (3.24%). Conclusion: The results data obtained in the present study suggest that essential oil possesses strong medicinal activities can be utilized for treatment of diseases.

Journal of pharmacology & clinical research, Oct 25, 2017

Medicinal plants play a pivotal role in the health care of ancient and modern cultures. The plant... more Medicinal plants play a pivotal role in the health care of ancient and modern cultures. The plant Medicinal plants play a pivotal role in the health care of ancient and modern cultures. The plant Leptodermis lanceolata including leaves, stem flower, were extracted by hydro distillation method for 6 hours using Clevenger apparatus The hydro-distilled essential oil of Leptodermis lanceolata has been examined by means of gas chromatography-mass spectrometry (GC-MS). Take 1.0 ml E. Oil of various code samples and mixed with in 4.0 ml DMSO (Dimethyl sulfoxide). The prepared extract was used for the determination of antioxidant activity (i.e., DPPH assay & ABTS assay) in among samples. The hydro-distilled essential oil of Leptodermis lanceolata has been examined by means of gas chromatography-mass spectrometry (GC-MS).Thirty-four constituents have been identified representing 72.29% of the total oil. The main compounds were phytol 19.44%, Linalool 12.12%, n-Hexadecanoic acid 7.55%, 4(E)-4-Hexenyl acetate 4.49%, the compound in minor amount were γ-Cadinene 0.21%, Neryl acetone 0.32%, 4,6-Dimethyl dodecane 0.32%, The oxygenated diterpene (36.68%), and oxygenated monoterpene (20%), found in the oil as major components while sesquterpenes (5.72%),were minor components. The results data obtained in the present study suggest that some essential oils possess strong medicinal activities, which can be utilized for treatment of certain diseases.

Revista Brasileira de Saúde e Produção Animal, 2018

SUMMARY Resistance to use antifungal drugs is a great concern seeking for scientists to discover ... more SUMMARY Resistance to use antifungal drugs is a great concern seeking for scientists to discover new products to treat fungal infections. The aim of this study was to evaluate the antioxidant and antifungal activities of essential oils and extracts of Plectranthus grandis and Plectranthus ornatus against Trichophyton rubrum and Microsporum canis dermatophytes strains. Extracts were obtained from leaves by maceration in ethanol (96%) during 7 days. The oils were obtained by hydrodistillation and analyzed by gas chromatography/mass spectrometry. A total of 25 components were identified, as major constituents the sesquiterpenes β-caryophyllene, α-copaene, germacrene, β-caryophyllene and caryophyllene oxide. Antioxidant activities were evaluated using DPPH scavenging assay and antifungal action was determined by the broth microdilution method. The decocts obtained from the extraction of essential oil presented a greater antioxidant action when compared with the essential oils, with IC50...

Journal of Analytical & Pharmaceutical Research, 2017

Antioxidant phytochemicals such as vitamin C, β-carotene, lutein, α-tocopherol, amino acid and to... more Antioxidant phytochemicals such as vitamin C, β-carotene, lutein, α-tocopherol, amino acid and total phenolics, were estimated in some medicinal plants using a reverse phase HPLC system. Wild plants play a vital role in the health security of Himalayan inhabitants and constitute significantly to their diet. Amino acid was analysis performed using the Waters Associates PICO-TAG method. The total amino acid content in P. indica was 58.80mg amino acid/g sample (dry weight), E. thymifolia was 123.92mg amino acid/g sample (dry weight) and P. hirta was 225.73mg amino acid/g sample (dry weight). The total essential amino acids in P. indica, E. thymifolia and P. hirta were 33.58, 57.99 and 145.82mg amino acid/g respectively. Among the three investigated plants, Carotenoids viz xanthophylls content was found 0.13 to 151.01 mg/100g dry weight basis. The maximum xanthophylls content was found in P. indica leaves and minimum in P. hirta rhizomes. The β-carotene content varies from 4.62-374.55mg/100g on dry weight basis. DL-α-tocopherol in these medicinal plants was found 13.48mg/100g, 24.95mg/100g and 9.13mg/100g on dry weight basis in P. hirta, E. thymifolia and P. indica respectively. This study discovers amino acid and antioxidant content in medicinal plants. Such information will increase the understanding of the faction of these antioxidant phytochemical in lowering incidence of ageing and other chronic diseases. Results of our study suggest the great value of these species for use in pharmacy and phytotherapy. Based on this information, it could be concluded that this plant is natural sources of antioxidant substances of high importance.

Journal of Pharmaceutics & Pharmacology, 2018

Introduction: Polygonum amplexicaule (Polygonaceae) is an herbal drug used to treat fractures, rh... more Introduction: Polygonum amplexicaule (Polygonaceae) is an herbal drug used to treat fractures, rheumatism, osteoporosis, muscle injuries and pain. It has also been reported to be effective to treat atherosclerosis and its antibiotic and antivirus effects. Methods: The plant Polygonum amplexicaule including leaves, stem, and flowers were extracted by hydro distillation method for 6 hours using Clevenger apparatus. Results: Total fifty one compounds were identified constituting 96.09% of the total oil. The main compounds was Geraniol (19.91%), β-Linalool (19.63), Citronellol (16.22%), 2-Methyl-6-hepten-1-ol (7.77%), Heptan-2-ol (5.66). Conclusion: The results data obtained in the present study suggest that an essential oil and whole plant possesses strong medicinal activities can be utilized for treatment of diseases.

World Journal of Pharmaceutical Research, 2017

The hydro-distilled essential oil of Excocaria acerifolia has been examined by means of gas chrom... more The hydro-distilled essential oil of Excocaria acerifolia has been examined by means of gas chromatography-mass spectrometry (GCMS). The oil constituents were identified according to their mass spectra and their relative retention indices determined on a non-polar stationary phase capillary column. Fifty-seven constituents have been identified representing 93.37% of the total oil. The main compounds in major amounts were Isopropyl isothiocyanate (14.03%), (E)- Caryophyllen (11.65%), Benzyl isothiocyanate (9.32%), 1, 3, 8- paramenthatriene (6.61%), the compound in trace or in minor amount were n-hexanol (0.06%), 2-Ethyl-5-methyl tetrahydrofuran (0.06%), the oil was found rich in oxygenated mono and sesquiterpene hydrocarbons.

World Journal of Pharmaceutical Research, 2017

Research Journal of Phytochemistry, 2014

Research Journal of Phytochemistry, 2014

Biomedical Chromatography, 2009

The flavonoid content of several methanolic extract fractions of Navel orange peel (flavedo and a... more The flavonoid content of several methanolic extract fractions of Navel orange peel (flavedo and albedo of Citrus sinensis) cultivated in Crete (Greece) was first analysed phytochemically and then assessed for its antioxidant activity in vitro. The chemical structures of the constituents fractionated were originally determined by comparing their retention times and the obtained UV spectral data with the available bibliographic data and further verified by detailed LC-DAD-MS (ESI+) analysis. The main flavonoid groups found within the fractions examined were polymethoxylated flavones, O-glycosylated flavones, C-glycosylated flavones, O-glycosylated flavonols, O-glycosylated flavanones and phenolic acids along with their ester derivatives. In addition, the quantitative HPLC analysis confirmed that hesperidin is the major flavonoid glycoside found in the orange peel. Interestingly enough, its quantity at 48 mg/g of dry peel permits the commercial use of orange peel as a source for the production of hesperidin. The antioxidant activity of the orange peel methanolic extract fractions was evaluated by applying two complementary methodologies, DPPH • assay and the Co(II)/EDTA-induced luminol chemiluminescence approach. Overall, the results have shown that orange peel methanolic extracts possess moderate antioxidant activity as compared with the activity seen in tests where the corresponding aglycones, diosmetin and hesperetin were assessed in different ratios.

ethnoleaflets.com

... Corresponding author Arvind Bhatt, School of Biological & Conservation Sciences. Universi... more ... Corresponding author Arvind Bhatt, School of Biological & Conservation Sciences. University of Kwazulu-Natal , Private Bag X54001, Durban 4000, South Africa. ... Kala, CP . 2002. Medicinal Plants of Indian Trans-Himalaya Bishen Singh Mahendra Pal Singh, Dehradun , India ,. ...

Journal of Medicinal Plants Research, 2017

The families Violaceae (alternatively known as Alsodeiace or Leoniaceae or Retrosepalaceae) compr... more The families Violaceae (alternatively known as Alsodeiace or Leoniaceae or Retrosepalaceae) comprise twenty genera and about 800 species. Viola serpens belongs to family Violaceae and commonly known as "Banafsa". It is a small glabrous, perennial herb, which is found throughout India in moist woods and hilly districts. The essential oil of aerial parts of V. serpens, were extracted by steam distillation. The quantitative and qualitative analysis of volatile essential oil constituents of the plant was done by Gas Chromatography (GC) and GC-Mass Spectrometry. A total of 50 components of the essential oil of V. serpens were identified, accounting for 81.38% of the total oil. The main compounds found were Bis (2ethylhexyl) maleate (15.62%), 2, 4, 4, 6-Tetramethyl-2-heptene (11.52%), Hexen-3-ol (6.56%), and Cis Verbeno (l 4.77%). The chemical constituents in the essential oil from V. serpens were identified in the following classes or groups of chemical compounds, such as monoterpens, sesquiterpenes volatile organic compounds and their oxygenated hydrocarbons. Therefore, the essential constituents could be used as antioxidant, antifungal or antimicrobial agent in new drugs preparation for therapy of infectious diseases.