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Papers by Kurnia Nisa Kinasih

Research paper thumbnail of Identification of Escherichia coli Multidrug Resistance in Cattle in Abattoirs

Jurnal medik veteriner‬, Apr 1, 2024

Escherichia coli is a typical flora of an animals' digestive tract. Based on these details, this ... more Escherichia coli is a typical flora of an animals' digestive tract. Based on these details, this study was done on the detection and identification of multidrug-resistant E. coli in cattle in Surabaya abattoirs. Each cattle rectum swab sample was streaked into EMB agar media and followed by Gram staining. The IMViC test was used to confirm the presence of E. coli. Based on morphological culture features, Gram staining, and biochemical testing, the sample examination results revealed that 41 samples (41%) of the 100 cattle rectal swab samples that were isolated were confirmed to be positive for E. coli. The profile of antibiotic resistance from the results of the E. coli resistance test to antibiotics showed that out of a total of 41 isolates of E. coli, 12 isolates (29.27%) were detected as resistant to 1 class of antibiotics tested, while 2 isolates (4.88%) were resistant to 2 classes of antibiotics, and 3 isolates (7.32%) were confirmed to be multidrug resistance (MDR).

Research paper thumbnail of Implementasi Program Sosialisasi Pengolahan Dan Pemasaran Produk Olahan Ikan Air Tawar Dalam Upaya Meningkatkan Pendapatan Masyarakat Terhadap Perekonomian DI Desa Tapen Kecamatan Tapen Kabupaten Bondowoso

Jurnal Layanan Masyarakat (Journal of Public Services), 2021

The freshwater fishery sector is one of the potentials possessed by the village of Tapen due to i... more The freshwater fishery sector is one of the potentials possessed by the village of Tapen due to its location near the river and the Sampean Baru dam, which is the largest river in Bondowoso Regency. However, the income of the people with the livelihood of freshwater fish fishermen in Tapen Village is not optimal because the community does not understand how to process and market processed freshwater fish products in order to increase the selling value of fish. Efforts to increase community income through the fisheries sector carried out by the 60 KKN BBM Uniar period eke-63 are socialization of processing and marketing of processed freshwater fish products. The activity was carried out with a presentation method regarding the processing and marketing of processed freshwater fish products as well as a demonstration on the process of making processed freshwater fish products in the form of catfish dumplings and tilapia nuggets. The socialization participants were PKK women, village ca...

Research paper thumbnail of Molecular detection of Klebsiella pneumoniae producing extended-spectrum beta-lactamase isolated from bat feces from the Tanjung Ringgit bat cave, Lombok Island, Indonesia

International Journal of One Health, Apr 5, 2024

Background and Aim: Bats are a reservoir for the pathogenic bacteria Klebsiella pneumoniae and ca... more Background and Aim: Bats are a reservoir for the pathogenic bacteria Klebsiella pneumoniae and can spread it through feces that fall in nests/caves, carried, and dropped while they fly near human settlements, and from their saliva. The emergence and spread of multidrug resistance (MDR) strains of K. pneumoniae indicate that resistant to antibiotics, especially extended-spectrum beta-lactamase (ESBL), is considered an important global health threat. The aim of this study was to determine the presence of the gene encoding extended beta-lactamase in K. pneumoniae isolated from fresh bat feces collected from the Tanjung Ringgit bat cave, East Lombok.

Materials and Methods: In this study, 150 fresh fecal samples were analyzed using standard microbiological techniques for the presence of K. pneumoniae. K. pneumoniae-positive isolates were subjected to antibiotic sensitivity testing, followed by molecular detection using polymerase chain reaction.

Results: This study showed that 14 (9.3%) of 150 samples were positive for K. pneumoniae. Ten of the 14 samples (71.4%) were MDR isolates and 6 (42.9%) had the blaSHV gene identified.

Conclusion: The presence of K. pneumoniae isolated from fresh bat feces, which is MDR and has the blaSHV gene encoding ESBL indicates that bats can be a reservoir for the transmission of MDR and ESBL bacteria has an impact on public health in the study area.

Keywords: bat, blaSHV, extended-spectrum beta-lactamase, Klebsiella pneumoniae, public health.

Research paper thumbnail of Identification of Escherichia coli Multidrug Resistance in Cattle in Abattoirs

Jurnal medik veteriner‬, Apr 1, 2024

Escherichia coli is a typical flora of an animals' digestive tract. Based on these details, this ... more Escherichia coli is a typical flora of an animals' digestive tract. Based on these details, this study was done on the detection and identification of multidrug-resistant E. coli in cattle in Surabaya abattoirs. Each cattle rectum swab sample was streaked into EMB agar media and followed by Gram staining. The IMViC test was used to confirm the presence of E. coli. Based on morphological culture features, Gram staining, and biochemical testing, the sample examination results revealed that 41 samples (41%) of the 100 cattle rectal swab samples that were isolated were confirmed to be positive for E. coli. The profile of antibiotic resistance from the results of the E. coli resistance test to antibiotics showed that out of a total of 41 isolates of E. coli, 12 isolates (29.27%) were detected as resistant to 1 class of antibiotics tested, while 2 isolates (4.88%) were resistant to 2 classes of antibiotics, and 3 isolates (7.32%) were confirmed to be multidrug resistance (MDR).

Research paper thumbnail of Implementasi Program Sosialisasi Pengolahan Dan Pemasaran Produk Olahan Ikan Air Tawar Dalam Upaya Meningkatkan Pendapatan Masyarakat Terhadap Perekonomian DI Desa Tapen Kecamatan Tapen Kabupaten Bondowoso

Jurnal Layanan Masyarakat (Journal of Public Services), 2021

The freshwater fishery sector is one of the potentials possessed by the village of Tapen due to i... more The freshwater fishery sector is one of the potentials possessed by the village of Tapen due to its location near the river and the Sampean Baru dam, which is the largest river in Bondowoso Regency. However, the income of the people with the livelihood of freshwater fish fishermen in Tapen Village is not optimal because the community does not understand how to process and market processed freshwater fish products in order to increase the selling value of fish. Efforts to increase community income through the fisheries sector carried out by the 60 KKN BBM Uniar period eke-63 are socialization of processing and marketing of processed freshwater fish products. The activity was carried out with a presentation method regarding the processing and marketing of processed freshwater fish products as well as a demonstration on the process of making processed freshwater fish products in the form of catfish dumplings and tilapia nuggets. The socialization participants were PKK women, village ca...

Research paper thumbnail of Molecular detection of Klebsiella pneumoniae producing extended-spectrum beta-lactamase isolated from bat feces from the Tanjung Ringgit bat cave, Lombok Island, Indonesia

International Journal of One Health, Apr 5, 2024

Background and Aim: Bats are a reservoir for the pathogenic bacteria Klebsiella pneumoniae and ca... more Background and Aim: Bats are a reservoir for the pathogenic bacteria Klebsiella pneumoniae and can spread it through feces that fall in nests/caves, carried, and dropped while they fly near human settlements, and from their saliva. The emergence and spread of multidrug resistance (MDR) strains of K. pneumoniae indicate that resistant to antibiotics, especially extended-spectrum beta-lactamase (ESBL), is considered an important global health threat. The aim of this study was to determine the presence of the gene encoding extended beta-lactamase in K. pneumoniae isolated from fresh bat feces collected from the Tanjung Ringgit bat cave, East Lombok.

Materials and Methods: In this study, 150 fresh fecal samples were analyzed using standard microbiological techniques for the presence of K. pneumoniae. K. pneumoniae-positive isolates were subjected to antibiotic sensitivity testing, followed by molecular detection using polymerase chain reaction.

Results: This study showed that 14 (9.3%) of 150 samples were positive for K. pneumoniae. Ten of the 14 samples (71.4%) were MDR isolates and 6 (42.9%) had the blaSHV gene identified.

Conclusion: The presence of K. pneumoniae isolated from fresh bat feces, which is MDR and has the blaSHV gene encoding ESBL indicates that bats can be a reservoir for the transmission of MDR and ESBL bacteria has an impact on public health in the study area.

Keywords: bat, blaSHV, extended-spectrum beta-lactamase, Klebsiella pneumoniae, public health.