Lê Phát - Academia.edu (original) (raw)
Papers by Lê Phát
Bulletin of Experimental Biology and Medicine, 1989
Much attention is currently being paid to problems connected with the study of the mechanisms del... more Much attention is currently being paid to problems connected with the study of the mechanisms delaying maturation of the fetal lungs in the intrauterine period, one result of which, according to a number of authorities, is the development of neonatal respiratory distress syndrome [6], in the pathogenesis of which a leading role is played by immaturity of the lung surfactant (LS) system. Among the factors inhibiting maturation of the fetal lungs and of the LS system are extragenital maternal pathology [5] and also intrauterine fetal hypoxia [1-4], arising in various pathological states of the mother during pregnancy. The aim of this investigation was to study the state of surface activity of LS and the ultrastructure of the type II alveolocytes of the fetal and neonatal lungs, responsible for LS production, during experimental maternal pneumonia. EXPERIMENTAL METHOD The maximal and minimal surface tension (ST) of the surface-active fraction of animal lung extracts was determined by means of Wilhelmy's scales. The content of total lipids and phospholipids of LS was determined by biochemical methods. The lungs of 14 live-born and five stillborn guinea pigs (19 observations altogether), born to eight mothers, and divided into four groups, were subjected to histological and electron-microscopic investigation. Group 1 consisted of eight animals: five stillborn and three live born, to three mothers on the 64th-65th days of pregnancy, into which sterile threads were inserted transtracheally under open ether anesthesia on the 58th-60th days of pregnancy. Group 2 consisted of five live-born guinea pigs, born to two mothers, into which the sterile threads were inserted transtracheally before the beginning of pregnancy. To rule out any possible effect of brief ether anesthesia in the mother on the state of the LS system of the fetus, the lungs of two live-born guinea pigs, born to the same Legend. i. STmi n) minimal ST, IS) index of stability. 2. Significance of differences (p) given in text after Table i. 3. Data on concentrations of cholesterol, neutral lipids, and various phospholipid fractions identified by thin-layer chromatography are not given in this Table.
The New England …, 2004
From the Hospital for Tropical Diseases (TTH, NTD, PPM, NVC, DBK, NT Truong) and the Oxford Unive... more From the Hospital for Tropical Diseases (TTH, NTD, PPM, NVC, DBK, NT Truong) and the Oxford University Clinical Research Unit at the Hospital for Tropical Diseases (CD, TTT, MJ, CS, JF); Pedi- atric Hospital Number Two (VCD); Health Service of Ho Chi Minh City (LTG); ...
Bulletin of Experimental Biology and Medicine, 1989
Much attention is currently being paid to problems connected with the study of the mechanisms del... more Much attention is currently being paid to problems connected with the study of the mechanisms delaying maturation of the fetal lungs in the intrauterine period, one result of which, according to a number of authorities, is the development of neonatal respiratory distress syndrome [6], in the pathogenesis of which a leading role is played by immaturity of the lung surfactant (LS) system. Among the factors inhibiting maturation of the fetal lungs and of the LS system are extragenital maternal pathology [5] and also intrauterine fetal hypoxia [1-4], arising in various pathological states of the mother during pregnancy. The aim of this investigation was to study the state of surface activity of LS and the ultrastructure of the type II alveolocytes of the fetal and neonatal lungs, responsible for LS production, during experimental maternal pneumonia. EXPERIMENTAL METHOD The maximal and minimal surface tension (ST) of the surface-active fraction of animal lung extracts was determined by means of Wilhelmy's scales. The content of total lipids and phospholipids of LS was determined by biochemical methods. The lungs of 14 live-born and five stillborn guinea pigs (19 observations altogether), born to eight mothers, and divided into four groups, were subjected to histological and electron-microscopic investigation. Group 1 consisted of eight animals: five stillborn and three live born, to three mothers on the 64th-65th days of pregnancy, into which sterile threads were inserted transtracheally under open ether anesthesia on the 58th-60th days of pregnancy. Group 2 consisted of five live-born guinea pigs, born to two mothers, into which the sterile threads were inserted transtracheally before the beginning of pregnancy. To rule out any possible effect of brief ether anesthesia in the mother on the state of the LS system of the fetus, the lungs of two live-born guinea pigs, born to the same Legend. i. STmi n) minimal ST, IS) index of stability. 2. Significance of differences (p) given in text after Table i. 3. Data on concentrations of cholesterol, neutral lipids, and various phospholipid fractions identified by thin-layer chromatography are not given in this Table.
The New England …, 2004
From the Hospital for Tropical Diseases (TTH, NTD, PPM, NVC, DBK, NT Truong) and the Oxford Unive... more From the Hospital for Tropical Diseases (TTH, NTD, PPM, NVC, DBK, NT Truong) and the Oxford University Clinical Research Unit at the Hospital for Tropical Diseases (CD, TTT, MJ, CS, JF); Pedi- atric Hospital Number Two (VCD); Health Service of Ho Chi Minh City (LTG); ...