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Papers by Lana Pađen
PROCEEDINGS OF THE 2nd INTERNATIONAL SCIENTIFIC AND PROFESSIONAL MEETING ON REPTILES AND EXOTIC ANIMALS “REPTILIA”, 2021
9TH INTERNATIONAL CONGRESS „VETERINARY SCIENCE AND PROFESSION” Book Of Abstracts, 2021
Hrvatski veterinarski vjesnik, Mar 14, 2021
Metabolites
The composition and properties of the milk fat of mares’ milk is interesting from a nutritional s... more The composition and properties of the milk fat of mares’ milk is interesting from a nutritional standpoint. The aim of this study was to determine the nutritional value of Lipizzaner mare’s milk for possible human consumption and identity the influence of the parity and stage of lactation on its fatty acid (FA) composition. This study was conducted on 17 Lipizzaner mares from a state stud farm in the Slavonian region (eastern Croatia). Mares were hand-milked twice during lactation in the fourth and sixth months. Significantly higher contents of MUFAs (monounsaturated fatty acids) and PUFAs (polyunsaturated fatty acids) and lower ratios of n-6/n-3 were found in the fourth month of lactation. This parity significantly affected the PUFA/SFA ratio (polyunsaturated fatty acids/saturated fatty acids), with lower values found in older mares. The fatty acid composition of mare’s milk that is especially high in UFAs (unsaturated fatty acids) is considered to be beneficial for consumers.
Vuk (Canis lupus) zasticena je vrsta u Hrvatskoj, te je za stete koje vuk napravi stanovnistvu dr... more Vuk (Canis lupus) zasticena je vrsta u Hrvatskoj, te je za stete koje vuk napravi stanovnistvu država dužna isplatiti odstetu. Kod utvrđivanja stete na stoci, u mnogim slucajevima je tesko odrediti da li je pocinitelj bio vuk ili pas (Canis lupus familiaris), a cesto se na mjestu gdje je steta pocinjena može naci bioloski materijal pocinitelja. Nadalje, u nekim slucajevima je na temelju morfoloskih karakteristika pronađenih lesina vrlo tesko razlikovati vuka i psa. U ovom radu smo željeli razviti molekularnu metodu razlikovanja vuka od pasa, koristenjem kontrolne regije mitohondrijske DNA. Iz cetiri uzorka (vuk, dva psa i jedan sumnjivi, za koji se nije znalo da li je vuk ili pas) izolirali smo DNA, sekvencirali dio kontrolne regije mitohondrijske DNA (235 bp dugacke), te ih usporedili zajedno s jos cetiri sekvence (tri vuka i jedne psa) uzete iz genske baze podataka, GenBank. Za analizu sekvenci koristili smo programe BioEdit i Mega3. Pronasli smo specificna mjesta u kontrolnim reg...
ISJ-Invertebrate Survival Journal, 2017
The aim of this study was to determine interspecies and gender differences in the lipid metabolis... more The aim of this study was to determine interspecies and gender differences in the lipid metabolism and hemolymph oxidation stability in intermoult period of the European spider crab (M. squinado), the warty crab (E. verrucosa) and the European spiny lobster (P. elephas). In hemolymph samples activities of superoxide dismutase (SOD), glutathione peroxidase (GSH-Px) and paraoxonase 1 (PON 1), as well as hemolymph cholesterol, triacylglycerol and non-esterified fatty acid (NEFA) concentrations were determined. The hemolymph of M. squinado showed the highest levels of SOD activity, cholesterol and triacylglycerol and the lowest levels of PON 1 activity compared to values determined in the hemolymph of E. verrucosa and P. elephas. Gender differences were determined only in M. squinado samples. Males had significantly higher activity of PON 1 and lower NEFA values compared to females. M. squinado hemolymph lipid metabolism and oxidation stability in intermoult period could be attributed t...
Lipids are an important component of the follicular fluid (FF) as a cellular energy source and als... more Lipids are an important component of the follicular fluid (FF) as a cellular energy source and also have important biological functions in cell membrane biogenesis and signalling. Fatty acids (FAs) in FF are present in esterifed form as triacylglycerol (TAG), cholesterol esters and phospholipids (PHL), and as nonesterifed FAs (NEFAs). The aim of the study was to determine the lipid composition of bovine FF during folliculogenesis. Bovine ovaries were collected at a slaughterhouse, and follicular fluid samples were obtained from follicles of three different sizes ( 5 mm N=22 ; and >10 mm N=23). Concentrations of TAG, PHL, total cholesterol (TC), low- density lipoprotein cholesterol (LDL- C), high- density lipoprotein cholesterol (HDL- C), NEFA and FAs in FF were analysed. The saturated fatty acids were dominant in bovine FF and the most common was myristic acid (C14:0). Polyunsaturated fatty acids were the second most represented, with linoleic acid (C18:2) in the highest percentage....
PROCEEDINGS OF THE 2nd INTERNATIONAL SCIENTIFIC AND PROFESSIONAL MEETING ON REPTILES AND EXOTIC ANIMALS “REPTILIA”, 2021
9TH INTERNATIONAL CONGRESS „VETERINARY SCIENCE AND PROFESSION” Book Of Abstracts, 2021
Hrvatski veterinarski vjesnik, Mar 14, 2021
Metabolites
The composition and properties of the milk fat of mares’ milk is interesting from a nutritional s... more The composition and properties of the milk fat of mares’ milk is interesting from a nutritional standpoint. The aim of this study was to determine the nutritional value of Lipizzaner mare’s milk for possible human consumption and identity the influence of the parity and stage of lactation on its fatty acid (FA) composition. This study was conducted on 17 Lipizzaner mares from a state stud farm in the Slavonian region (eastern Croatia). Mares were hand-milked twice during lactation in the fourth and sixth months. Significantly higher contents of MUFAs (monounsaturated fatty acids) and PUFAs (polyunsaturated fatty acids) and lower ratios of n-6/n-3 were found in the fourth month of lactation. This parity significantly affected the PUFA/SFA ratio (polyunsaturated fatty acids/saturated fatty acids), with lower values found in older mares. The fatty acid composition of mare’s milk that is especially high in UFAs (unsaturated fatty acids) is considered to be beneficial for consumers.
Vuk (Canis lupus) zasticena je vrsta u Hrvatskoj, te je za stete koje vuk napravi stanovnistvu dr... more Vuk (Canis lupus) zasticena je vrsta u Hrvatskoj, te je za stete koje vuk napravi stanovnistvu država dužna isplatiti odstetu. Kod utvrđivanja stete na stoci, u mnogim slucajevima je tesko odrediti da li je pocinitelj bio vuk ili pas (Canis lupus familiaris), a cesto se na mjestu gdje je steta pocinjena može naci bioloski materijal pocinitelja. Nadalje, u nekim slucajevima je na temelju morfoloskih karakteristika pronađenih lesina vrlo tesko razlikovati vuka i psa. U ovom radu smo željeli razviti molekularnu metodu razlikovanja vuka od pasa, koristenjem kontrolne regije mitohondrijske DNA. Iz cetiri uzorka (vuk, dva psa i jedan sumnjivi, za koji se nije znalo da li je vuk ili pas) izolirali smo DNA, sekvencirali dio kontrolne regije mitohondrijske DNA (235 bp dugacke), te ih usporedili zajedno s jos cetiri sekvence (tri vuka i jedne psa) uzete iz genske baze podataka, GenBank. Za analizu sekvenci koristili smo programe BioEdit i Mega3. Pronasli smo specificna mjesta u kontrolnim reg...
ISJ-Invertebrate Survival Journal, 2017
The aim of this study was to determine interspecies and gender differences in the lipid metabolis... more The aim of this study was to determine interspecies and gender differences in the lipid metabolism and hemolymph oxidation stability in intermoult period of the European spider crab (M. squinado), the warty crab (E. verrucosa) and the European spiny lobster (P. elephas). In hemolymph samples activities of superoxide dismutase (SOD), glutathione peroxidase (GSH-Px) and paraoxonase 1 (PON 1), as well as hemolymph cholesterol, triacylglycerol and non-esterified fatty acid (NEFA) concentrations were determined. The hemolymph of M. squinado showed the highest levels of SOD activity, cholesterol and triacylglycerol and the lowest levels of PON 1 activity compared to values determined in the hemolymph of E. verrucosa and P. elephas. Gender differences were determined only in M. squinado samples. Males had significantly higher activity of PON 1 and lower NEFA values compared to females. M. squinado hemolymph lipid metabolism and oxidation stability in intermoult period could be attributed t...
Lipids are an important component of the follicular fluid (FF) as a cellular energy source and als... more Lipids are an important component of the follicular fluid (FF) as a cellular energy source and also have important biological functions in cell membrane biogenesis and signalling. Fatty acids (FAs) in FF are present in esterifed form as triacylglycerol (TAG), cholesterol esters and phospholipids (PHL), and as nonesterifed FAs (NEFAs). The aim of the study was to determine the lipid composition of bovine FF during folliculogenesis. Bovine ovaries were collected at a slaughterhouse, and follicular fluid samples were obtained from follicles of three different sizes ( 5 mm N=22 ; and >10 mm N=23). Concentrations of TAG, PHL, total cholesterol (TC), low- density lipoprotein cholesterol (LDL- C), high- density lipoprotein cholesterol (HDL- C), NEFA and FAs in FF were analysed. The saturated fatty acids were dominant in bovine FF and the most common was myristic acid (C14:0). Polyunsaturated fatty acids were the second most represented, with linoleic acid (C18:2) in the highest percentage....