Larissa de Paula - Academia.edu (original) (raw)

Papers by Larissa de Paula

Research paper thumbnail of Glutathione and S-nitrosoglutathione in alginate/chitosan nanoparticles: Cytotoxicity

Journal of Physics: Conference Series, 2011

Nitric oxide (NO) is involved in several physiological processes, such as the control of vascular... more Nitric oxide (NO) is involved in several physiological processes, such as the control of vascular tone, the immune response and the wound healing process. Thus, there is a great interest in the development of NO-releasing drugs and in matrices which are able to stabilize and release NO locally in different tissues. Thiols, such as glutathione (GSH), are ready nitrosated to form the NO donors S-nitrosothiols (RSNOs). In this work, GSH, a precursor of the NO donor S-nitrosoglutathione (GSNO), was encapsulated into a mucoadhesive combination of alginate/chitosan nanoparticles. The encapsulated GSH was nitrosated in the alginate/chitosan nanoparticles by adding sodium nitrite, leading to the formation of encapsulated GSNO. The cytotoxicity characterization of the nanoparticles containing either GSH or GSNO showed that these materials were completely non cytotoxic to cellular viability. These results show that this novel nanostructure biomaterial has a great potential to be use in biomedical applications where NO has a therapeutical effect.

Research paper thumbnail of New Strategy for Controlled Release of Nitric Oxide

Journal of Nano Research, 2012

Nitric oxide (NO) is involved in several physiological processes, such as the control of vascular... more Nitric oxide (NO) is involved in several physiological processes, such as the control of vascular tone, the inhibition of platelet aggregation, smooth muscle cell replication, immune response and neuronal communication. Several pathologies have been associated to dysfunctions in the endogenous NO production. Thus, there is a great interest in the development of NO-releasing drugs and in matrices which are able to stabilize and release NO locally in different tissues. In this scenario, the preparation of NO-releasing nanomaterials, such as dendrimers, liposomes, metallic, silica, and polymeric nanoparticles, zeolites and metal organic frameworks, is a promising strategy for delivering NO in diverse applications, as discussed in this work.

Research paper thumbnail of Índices De Estresse Oxidativo Em Sujeitos Com Diferentes Níveis De Composição Corporal e Aderência a Prática De Atividade Física

ANTUNES NETO, J. M. F.; PAULA, L. B. Indices of oxidative stress in subjects with different level... more ANTUNES NETO, J. M. F.; PAULA, L. B. Indices of oxidative stress in subjects with different levels of body composition and tack the practice of physical activity. Brazilian Journal of Biomotricity, v. 5, n. 2, p. 117-131, 2011. The reactive oxygen species (ROS) are derived from exogenous sources or produced endogenously, as a normal consequence of cellular functions. The unbalance caused by the excess in the production of these species associated with the fall in the cellular antioxidant capacity provokes what we call oxidative stress; a process that generates, over all, alterations in the cellular membranes and in the genes structures, at extreme circumstances. The objective of this work consisted in investigating the oxidative stress potential in sedentary obese subjects, since the obesity became a public health problem, over all in industrialized countries. Thirty men volunteers (age 26,6 ± 3,5) participated of the study and have divided in three groups, in accordance with corpor...

Research paper thumbnail of In Vivo Evaluation of Complex Biogenic Silver Nanoparticle and Enoxaparin in Wound Healing

Journal of Nanomaterials, 2015

The burns treatment is difficult, uncomfortable for the patient, and expensive for health system.... more The burns treatment is difficult, uncomfortable for the patient, and expensive for health system. Due to antimicrobial properties of silver nanoparticles (AgNP), these particles can avoid bacterial infection in wound and accelerate the wound healing. Furthermore, the complexation of AgNP with enoxaparin (low molecular weight heparin) may improve the healing process of lesions due to anti-inflammatory and angiogenic activity of enoxaparin (Enox). The aim of this study was evaluated the activity and toxicity of biogenic AgNP and AgNP complexed with Enox inin vivoburn wound model. AgNP was produced by biosynthesis method usingFusarium oxysporum. AgNP (20–40 nm) exhibited high stability due to protein capping around the particles that was confirmed by TEM, fluorescence spectroscopy, and FTIR. The wound contraction inin vivomodel, after 28 days of treatment, was 55, 89, 91, and 95% for control, Enox, AgNP, and AgNP-Enox groups, respectively. No clear toxic effects in the biochemistry and...

Research paper thumbnail of Glutathione and S-nitrosoglutathione in alginate/chitosan nanoparticles: Cytotoxicity

Journal of Physics: Conference Series, 2011

Nitric oxide (NO) is involved in several physiological processes, such as the control of vascular... more Nitric oxide (NO) is involved in several physiological processes, such as the control of vascular tone, the immune response and the wound healing process. Thus, there is a great interest in the development of NO-releasing drugs and in matrices which are able to stabilize and release NO locally in different tissues. Thiols, such as glutathione (GSH), are ready nitrosated to form the NO donors S-nitrosothiols (RSNOs). In this work, GSH, a precursor of the NO donor S-nitrosoglutathione (GSNO), was encapsulated into a mucoadhesive combination of alginate/chitosan nanoparticles. The encapsulated GSH was nitrosated in the alginate/chitosan nanoparticles by adding sodium nitrite, leading to the formation of encapsulated GSNO. The cytotoxicity characterization of the nanoparticles containing either GSH or GSNO showed that these materials were completely non cytotoxic to cellular viability. These results show that this novel nanostructure biomaterial has a great potential to be use in biomedical applications where NO has a therapeutical effect.

Research paper thumbnail of Nanoparticles in treatment of thermal injured rats: Is it safe?

Journal of Physics: Conference Series, 2011

The aim of this study was to assess whether thermal trauma induced oxidative stress altered the b... more The aim of this study was to assess whether thermal trauma induced oxidative stress altered the balance between oxidant and antioxidant systems in the blood of burn wound rats in the absence and presence of silver nanoparticles and S-nitrosoglutathione, GSNO. Free silver nanoparticles, free GSNO and silver nanoparticles + GSNO had no cytotoxic effects. Under anesthesia, the shaved dorsum of the rats was exposed to 90ºC (burn group) water bath. Studied compounds were administered topically immediately and at 28 days after the burn injury, four times a day. Silver nanoparticles and silver nanoparticles + GSNO were no toxic in vitro and in vivo. There were no significant differences in the levels of urea, creatinine, aminotransferases and hematological parameters, in control-burn groups (free silver nanoparticles) and treated-burn groups (free GSNO or silver nanoparticles + GSNO). There were no differences in lipid peroxidation and in the levels of protein carbonyls and glutathione, used as oxidative stress markers. A little inflammatory cell response, papillary dermis vascularization, fibroblasts differentiated into contractile myofibroblasts and the presence of a large amount of extracellular matrix were evidenced in treated groups following skin injury. These results indicate that silver nanoparticles and GSNO may provide an effective action on wound healing.

Research paper thumbnail of New Strategy for Controlled Release of Nitric Oxide

Journal of Nano Research, 2012

Nitric oxide (NO) is involved in several physiological processes, such as the control of vascular... more Nitric oxide (NO) is involved in several physiological processes, such as the control of vascular tone, the inhibition of platelet aggregation, smooth muscle cell replication, immune response and neuronal communication. Several pathologies have been associated to dysfunctions in the endogenous NO production. Thus, there is a great interest in the development of NO-releasing drugs and in matrices which are able to stabilize and release NO locally in different tissues. In this scenario, the preparation of NO-releasing nanomaterials, such as dendrimers, liposomes, metallic, silica, and polymeric nanoparticles, zeolites and metal organic frameworks, is a promising strategy for delivering NO in diverse applications, as discussed in this work.

Research paper thumbnail of Development of a Sustained-release System for Nitric Oxide Delivery using Alginate/Chitosan Nanoparticles

Current Nanoscience, 2013

Research paper thumbnail of Glutathione and S-nitrosoglutathione in alginate/chitosan nanoparticles: Cytotoxicity

Journal of Physics: Conference Series, 2011

Nitric oxide (NO) is involved in several physiological processes, such as the control of vascular... more Nitric oxide (NO) is involved in several physiological processes, such as the control of vascular tone, the immune response and the wound healing process. Thus, there is a great interest in the development of NO-releasing drugs and in matrices which are able to stabilize and release NO locally in different tissues. Thiols, such as glutathione (GSH), are ready nitrosated to form the NO donors S-nitrosothiols (RSNOs). In this work, GSH, a precursor of the NO donor S-nitrosoglutathione (GSNO), was encapsulated into a mucoadhesive combination of alginate/chitosan nanoparticles. The encapsulated GSH was nitrosated in the alginate/chitosan nanoparticles by adding sodium nitrite, leading to the formation of encapsulated GSNO. The cytotoxicity characterization of the nanoparticles containing either GSH or GSNO showed that these materials were completely non cytotoxic to cellular viability. These results show that this novel nanostructure biomaterial has a great potential to be use in biomedical applications where NO has a therapeutical effect.

Research paper thumbnail of New Strategy for Controlled Release of Nitric Oxide

Journal of Nano Research, 2012

Nitric oxide (NO) is involved in several physiological processes, such as the control of vascular... more Nitric oxide (NO) is involved in several physiological processes, such as the control of vascular tone, the inhibition of platelet aggregation, smooth muscle cell replication, immune response and neuronal communication. Several pathologies have been associated to dysfunctions in the endogenous NO production. Thus, there is a great interest in the development of NO-releasing drugs and in matrices which are able to stabilize and release NO locally in different tissues. In this scenario, the preparation of NO-releasing nanomaterials, such as dendrimers, liposomes, metallic, silica, and polymeric nanoparticles, zeolites and metal organic frameworks, is a promising strategy for delivering NO in diverse applications, as discussed in this work.

Research paper thumbnail of Índices De Estresse Oxidativo Em Sujeitos Com Diferentes Níveis De Composição Corporal e Aderência a Prática De Atividade Física

ANTUNES NETO, J. M. F.; PAULA, L. B. Indices of oxidative stress in subjects with different level... more ANTUNES NETO, J. M. F.; PAULA, L. B. Indices of oxidative stress in subjects with different levels of body composition and tack the practice of physical activity. Brazilian Journal of Biomotricity, v. 5, n. 2, p. 117-131, 2011. The reactive oxygen species (ROS) are derived from exogenous sources or produced endogenously, as a normal consequence of cellular functions. The unbalance caused by the excess in the production of these species associated with the fall in the cellular antioxidant capacity provokes what we call oxidative stress; a process that generates, over all, alterations in the cellular membranes and in the genes structures, at extreme circumstances. The objective of this work consisted in investigating the oxidative stress potential in sedentary obese subjects, since the obesity became a public health problem, over all in industrialized countries. Thirty men volunteers (age 26,6 ± 3,5) participated of the study and have divided in three groups, in accordance with corpor...

Research paper thumbnail of In Vivo Evaluation of Complex Biogenic Silver Nanoparticle and Enoxaparin in Wound Healing

Journal of Nanomaterials, 2015

The burns treatment is difficult, uncomfortable for the patient, and expensive for health system.... more The burns treatment is difficult, uncomfortable for the patient, and expensive for health system. Due to antimicrobial properties of silver nanoparticles (AgNP), these particles can avoid bacterial infection in wound and accelerate the wound healing. Furthermore, the complexation of AgNP with enoxaparin (low molecular weight heparin) may improve the healing process of lesions due to anti-inflammatory and angiogenic activity of enoxaparin (Enox). The aim of this study was evaluated the activity and toxicity of biogenic AgNP and AgNP complexed with Enox inin vivoburn wound model. AgNP was produced by biosynthesis method usingFusarium oxysporum. AgNP (20–40 nm) exhibited high stability due to protein capping around the particles that was confirmed by TEM, fluorescence spectroscopy, and FTIR. The wound contraction inin vivomodel, after 28 days of treatment, was 55, 89, 91, and 95% for control, Enox, AgNP, and AgNP-Enox groups, respectively. No clear toxic effects in the biochemistry and...

Research paper thumbnail of Glutathione and S-nitrosoglutathione in alginate/chitosan nanoparticles: Cytotoxicity

Journal of Physics: Conference Series, 2011

Nitric oxide (NO) is involved in several physiological processes, such as the control of vascular... more Nitric oxide (NO) is involved in several physiological processes, such as the control of vascular tone, the immune response and the wound healing process. Thus, there is a great interest in the development of NO-releasing drugs and in matrices which are able to stabilize and release NO locally in different tissues. Thiols, such as glutathione (GSH), are ready nitrosated to form the NO donors S-nitrosothiols (RSNOs). In this work, GSH, a precursor of the NO donor S-nitrosoglutathione (GSNO), was encapsulated into a mucoadhesive combination of alginate/chitosan nanoparticles. The encapsulated GSH was nitrosated in the alginate/chitosan nanoparticles by adding sodium nitrite, leading to the formation of encapsulated GSNO. The cytotoxicity characterization of the nanoparticles containing either GSH or GSNO showed that these materials were completely non cytotoxic to cellular viability. These results show that this novel nanostructure biomaterial has a great potential to be use in biomedical applications where NO has a therapeutical effect.

Research paper thumbnail of Nanoparticles in treatment of thermal injured rats: Is it safe?

Journal of Physics: Conference Series, 2011

The aim of this study was to assess whether thermal trauma induced oxidative stress altered the b... more The aim of this study was to assess whether thermal trauma induced oxidative stress altered the balance between oxidant and antioxidant systems in the blood of burn wound rats in the absence and presence of silver nanoparticles and S-nitrosoglutathione, GSNO. Free silver nanoparticles, free GSNO and silver nanoparticles + GSNO had no cytotoxic effects. Under anesthesia, the shaved dorsum of the rats was exposed to 90ºC (burn group) water bath. Studied compounds were administered topically immediately and at 28 days after the burn injury, four times a day. Silver nanoparticles and silver nanoparticles + GSNO were no toxic in vitro and in vivo. There were no significant differences in the levels of urea, creatinine, aminotransferases and hematological parameters, in control-burn groups (free silver nanoparticles) and treated-burn groups (free GSNO or silver nanoparticles + GSNO). There were no differences in lipid peroxidation and in the levels of protein carbonyls and glutathione, used as oxidative stress markers. A little inflammatory cell response, papillary dermis vascularization, fibroblasts differentiated into contractile myofibroblasts and the presence of a large amount of extracellular matrix were evidenced in treated groups following skin injury. These results indicate that silver nanoparticles and GSNO may provide an effective action on wound healing.

Research paper thumbnail of New Strategy for Controlled Release of Nitric Oxide

Journal of Nano Research, 2012

Nitric oxide (NO) is involved in several physiological processes, such as the control of vascular... more Nitric oxide (NO) is involved in several physiological processes, such as the control of vascular tone, the inhibition of platelet aggregation, smooth muscle cell replication, immune response and neuronal communication. Several pathologies have been associated to dysfunctions in the endogenous NO production. Thus, there is a great interest in the development of NO-releasing drugs and in matrices which are able to stabilize and release NO locally in different tissues. In this scenario, the preparation of NO-releasing nanomaterials, such as dendrimers, liposomes, metallic, silica, and polymeric nanoparticles, zeolites and metal organic frameworks, is a promising strategy for delivering NO in diverse applications, as discussed in this work.

Research paper thumbnail of Development of a Sustained-release System for Nitric Oxide Delivery using Alginate/Chitosan Nanoparticles

Current Nanoscience, 2013