Laura Araujo - Academia.edu (original) (raw)
Papers by Laura Araujo
Revista Conectividad
Contextualizar históricamente los espacios museales ha sido un reto debido a la poca información ... more Contextualizar históricamente los espacios museales ha sido un reto debido a la poca información que se ha podido investigar sobre la constitución de algunos museos en el Ecuador, si algo se ha podido obtener es sobre el primer Museo Nacional que estuvo ubicado en la ciudad de Quito desde 1839. A raíz de los procesos independentistas que se empezaron a visibilizar en la ciudad de Guayaquil y debido a un importante auge comercial de muchos productos que empezaron a ingresar por el puerto, se crea el Museo Industrial representado por objetos representativos de la nación y de sus habitantes poco a poco se va fortaleciendo y enriqueciendo y es así que en el 1908 pasa a constituirse como Museo Municipal de Guayaquil cuyo objetivo fue mostrar las reliquias y memorias de nuestros antepasados. A través de esta investigación se pretende reflexionar sobre instituciones que busquen crear un sentimiento de identidad que permita mantener el legado patrimonial como evidencia de identidad territor...
Staphylococcus aureus es el patogeno causante de mastitis bovina con mayor prevalencia en nuestro... more Staphylococcus aureus es el patogeno causante de mastitis bovina con mayor prevalencia en nuestro pais, y suele vincularse a brotes de enfermedades transmitidas por los alimentos. Este patogeno cobra protagonismo por su capacidad de resistir a la terapia antibiotica y producir infecciones en humanos. La perdida de sensibilidad frente a los antimicrobianos es causa de preocupacion a nivel mundial, por lo cual el estudio de la antibioticorresistencia en microorganismos aislados de animales vinculados a la produccion de alimentos es fundamental para la vigilancia epidemiologica. Para realizar el estudio de fenotipos de resistencia, la tecnica de difusion en agar es la mas utilizada y el uso de tecnicas cuantitativas, como la determinacion de la concentracion inhibitoria minima (CIM) y la concentracion bactericida minima (CBM), aportan datos valiosos. El objetivo general es evaluar la susceptibilidad in vitro de cepas de S. aureus aisladas de muestras de leche de vacas provenientes de t...
La evaluacion de la condicion corporal (CC) en vacas es un indicador subjetivo de la cantidad de ... more La evaluacion de la condicion corporal (CC) en vacas es un indicador subjetivo de la cantidad de reservas energeticas del animal y uno de los mejores indicadores del estado nutricional. El monitoreo de la CC permite prever la produccion de leche y la eficiencia reproductiva, evaluar el estado nutricional y reducir la incidencia de enfermedades metabolicas. Uno de los metodos mas utilizados para medir la CC es la forma visual, con la utilizacion de una escala de 1 a 5. En los ultimos anos, han aparecido nuevas tecnologias que podrian reemplazar a la medicion visual y, en esta linea, la empresa DeLaval desarrollo una camara infrarroja que a traves del registro de imagenes 3D de la parte posterior del cuerpo de las vacas calcula un “indice de condicion corporal BCS”. Se trata de un metodo sistematico de estimacion de CC, y es una herramienta de gestion facil de usar. La enfermedad que mayores perdidas economicas genera en los establecimientos lecheros y en la industria lactea es la mas...
La tesi doctoral titulada "Auto-assemblatge de copolimers de bloc per modificacio quimica de... more La tesi doctoral titulada "Auto-assemblatge de copolimers de bloc per modificacio quimica de la superficie", presenta com a objectiu principal el desenvolupament, implementacio i caracteritzacio d'un metode de guiatge de copolimers de bloc basat en la modificacio quimica de la superficie. El desenvolupament d'aquest metode de nanofabricacio contribueix a la futura generacio de dispositius i circuits nanoelectronics. Primer de tot, es presenten els aspectes generals sobre l'auto-assemblatge dirigit de copolimers de bloc, aixi com el seu rol dins del futur de la nanoelectronica comparat amb altres tecnologies emergents. Despres, per tal d'entendre i determinar les interaccions que tenen lloc durant el proces d'auto-assemblatge, es dona una visio general sobre els processos quimics i fisics que tenen lloc en les pel·licules primes de copolimers de bloc. La part principal de la tesi es focalitza en l'estudi, desenvolupament i implementacio d'un meto...
Bovine mastitis is the infectious disease with the greatest impact on world dairy production. In ... more Bovine mastitis is the infectious disease with the greatest impact on world dairy production. In Argentina, Staphylococcus aureus (S. aureus) is the most important pathogen in terms of prevalence and pathogenicity. It can resist antimicrobial treatments through different mechanisms. Currently, there is great concern about antibiotic resistance, both in human medicine and animal health. In our country, there is scarce information about antimicrobial resistance on bovine mastitis strains. The main objective of the project was to evaluate the in Vitro susceptibility of S. aureus isolated from milk samples obtained in dairy farms located in Buenos Aires. Between 2015 and 2016, 109 isolates of milk samples were obtained from cows with clinical mastitis, subclinical and bulk tank milk samples. Until July 2016, the susceptibility of 44 isolates were evaluated following the recommendations of the Clinical and Laboratory Standards Institute (CLSI). The antimicrobial agents included in the st...
Allergologia et Immunopathologia, 2020
Please cite this article in press as: Araujo LML, et al. Identification of the major allergenic p... more Please cite this article in press as: Araujo LML, et al. Identification of the major allergenic proteins from silkworm moth (Bombyx mori) involved in respiratory allergic diseases. Allergol Immunopathol (Madr). 2020.
Resumos do..., 2019
Colorectal cancer (CRC) is the second most common malignant neoplasm in women and the third most ... more Colorectal cancer (CRC) is the second most common malignant neoplasm in women and the third most prevalent in men worldwide. In Brazil, about 36,000 new cases of CRC are estimated among men and women for 2018-2019. With advances in treatment, early CRC is being "reclassified" from a deadly disease to an illness that has great chance of cure. However, so far, the survivors who have completed treatment and are cancer-free, frequently suffer from late/longterm side effects, and some patients with metastasis have poor response to the therapies. In this context, the discovery of new therapeutic strategies is urgently needed. In this project we aimed to validate the hypothesis that 3-Bromopyruvate (3-BP), an alkylating agent, would be able to sensitize the human CRC cell lines to treatment with Vemurafenib, a BRAF inhibitor. Therefore, human CRC cell lines (HT-29 and HCT-116) were challenged with 3-BP followed by the treatment with Vemurafenib. Both cell lines were more responsive to Vemurafenib after sensitization with 3-BP (IC50 values were 7-and 1.4-fold lower than with Vemurafenib alone, for HT-29 and HCT-116 cells, respectively). The findings presented here, highlight the potential of 3-BP in increasing the response of metastatic cancer cells to Vemurafenib.
Animals, 2019
Body condition scoring (BCS) is the management practice of assessing body reserves of individual ... more Body condition scoring (BCS) is the management practice of assessing body reserves of individual animals by visual or tactile estimation of subcutaneous fat and muscle. Both high and low BCS can negatively impact milk production, disease, and reproduction. Visual or tactile estimation of subcutaneous fat reserves in dairy cattle relies on their body shape or thickness of fat layers and muscle on key areas of the body. Although manual BCS has proven beneficial, consistent qualitative scoring can be difficult to implement. The desirable BCS range for dairy cows varies within lactation and should be monitored at multiple time points throughout lactation for the most impact, a practice that can be hard to implement. However, a commercial automatic BCS camera is currently available for dairy cattle (DeLaval Body Condition Scoring, BCS DeLaval International AB, Tumba, Sweden). The objective of this study was to validate the implementation of an automated BCS system in a commercial setting...
Biophysical Journal, 2018
Advances in Patterning Materials and Processes XXXIV, 2017
Directed self-assembly of block copolymers is considered one of the candidates to fulfill the req... more Directed self-assembly of block copolymers is considered one of the candidates to fulfill the requirements of the next technological nodes [1,2]. Polymer domains are aligned by using a chemical and/or a topographical pre-pattern in which preferential surfaces to one of the two blocks or neutral wetting areas are created. In particular, polystyrene-block-polymetylmethacrylate (PS-b-PMMA) has been extensively studied during the last years showing strong capabilities to define periodic nanostructures. However, the relatively low Flory-Huggins parameter and, the resulting low segregation strength of PS-b-PMMA systems, limit their achievable resolution to around 11 nm [3]. The application of block copolymer on sub-10 nm technologies requires the development of the new block copolymer generation known as high- block copolymers. Additionally, an important requirement for their integration on the lithography roadmap is the capability of selectively remove one of the two blocks. The etch contrast between the two domains is typically low due to their organic chemistry. In this sense, selective sequential infiltration synthesis by ALD into one of the blocks can be used in order to incorporate an inorganic material. The formed organic/inorganic blend sustains better the plasma etching to remove the non-infiltrated organic block. In this contribution, we show the use of high- polystyrene-b-polylactide acid (PS-b-PLA) lamellar block copolymer for line/space applications. PS-b-PLA has a larger Flory-Huggins parameter (=0.218 at room temperature[4]) compared with PS-b-PMMA, allowing the size reduction of the self-assembled domains. The method to control the orientation of the polymer domains is similar to the one typically used for PS-b-PMMA. Chemical contrast and the subsequent alignment of the polymer domains are achieved by the definition of a chemical pre-pattern on a random copolymer PS-r-PMMA (48% of PS) (figure 1). The polymer brush is grafted on the substrate and then locally modified by the combination of e-beam lithography and soft oxygen plasma. Afterwards, the PS-b-PLA block copolymer is spin-coated and thermally annealed on the chemically pre-patterned substrate. A chemical contrast is observed between the modified and unmodified stripes. While, the lamellar domains are oriented perpendicular to the substrate on unmodified areas, PLA domains are strongly attracted to the O2 modified surfaces inducing a parallel orientation to the substrate. Additionally, the wetting behavior of the polymer domains is also studied through the difference of surface free energy between the substrate and each polymer block. The energy estimated by the Young´s equation [Δγ =γSA -γSB= γAB·cos(ØAB)], where γSA and γSB are the interface tensions between homo-polymers A and B with the substrate, and ØAB is the contact angle between A and B homo-polymers which is obtained in de-wetting experiments. Finally, sequential infiltration synthesis is used to selectively infiltrate alumina (Al3O2) on PLA domains (figure 2). A selective infiltration is achieved because the precursor molecules react with the carbonyl (C=O) groups that are only present in the PLA block. After five cycles of SIS, the SIS modified PLA domains become more resistant to O2 plasma etching than PS enabling the PS etching without using other kind of hard-masks. The research leading to these results received funding from the European Union’s Seventh Framework Program FP7/2007-2013, under the project CoLiSA and the European Nanoelectronics Initiative Advisory Council under the project PLACYD (ENIAC-2013-2). L. Evangelio acknowledges MECD for the PhD contract FPU13/03746 [1] R. Ruiz, H. Kang, F. A. Detcheverry, E. Dobisz, D. S. Kercher, T. R. Albrecht, J. J. de Pablo, P. F. Nealey, Science 321 (2008) 936-939. [2] M. Fernández-Regúlez, L, Evangelio, M. Lorenzoni, J. Fraxedas, F. Perez-Murano, ACS Appl. Mater. Interfaces 6 (2014) 21596-21602. [3] L. Wan, R. Ruiz, H. Gao, K. C. Patel, T. R. Albrecht, J. Yin, J. Kim, Y. Cao, G. Lin, ACS Nano 9 (2015) 7506-7514 [4] A. S. Zalusky, R. Olayo-Valles , J. H. Wolf , M. A. Hillmyer, J. Am. Chem. Soc. 124 (2002) 12761-12773
Revista da Escola de Enfermagem da USP, 2016
An essay that aims to reflect on the family experience of care in chronic situation, increasing t... more An essay that aims to reflect on the family experience of care in chronic situation, increasing the understanding of the family as the primary caregiver. It is based on comprehensive approach in studies conducted in three matrix searches from family care experiences. We have taken three axes to organize our reflections: a) conformation of family care in chronic situation, highlighting the multiple costs incurred to the family, which can exhaust the potential of care and establish or increase its vulnerability if it is not backed by networks support and sustenance; b) family rearrangements for the care, giving visibility to care cores in which many loved family members share the care, dynamic, plural and changeable way; c) self care modeling family care, pointing to the range of possibilities of the person taking care of diseased conditions supported by people close to them. We learn that the family takes care of itself in everyday life and in the illness experience, creating network...
Anuario De Investigacion Usal, Sep 24, 2014
La leche de alta calidad debe poseer una composición adecuada, estar libre de patógenos, sustanci... more La leche de alta calidad debe poseer una composición adecuada, estar libre de patógenos, sustancias extrañas, olores, sedimentos y debe tener un contenido bajo de bacterias contaminantes y células somáticas. El consumo de lácteos elaborados a partir de leche de baja calidad impacta negativamente en la salud de los consumidores (Errecalde,1996; Giesecke, 1983; & Heeschen et al.1985). Por el contrario, producir leche de buena calidad es un factor que estabiliza el desarrollo socio-económico en áreas rurales, particularmente en los países en desarrollo (De Boer, 1981; & Mc Dowell, 1981). Argentina es el segundo productor latinoamericano de leche cruda y es el onceavo a nivel mundial pero la producción primaria se encuentra dividida en dos grupos: uno de alta y otro de baja eficiencia. Según el INTA y en MAGyP, el mejoramiento de la calidad de leche es un punto crítico que los productores de menor eficiencia deben resolver para cumplir con la creciente demanda que se espera en el mundo (INTA-MAGYP, 2011). En nuestro país existen numerosos trabajos que caracterizan la calidad de la leche pero no hay
Enfermeria Cientifica, 2004
Dental Press Journal of Orthodontics, 2015
Objective: The aim of this study was to assess the effects of combined headgear used alone or in ... more Objective: The aim of this study was to assess the effects of combined headgear used alone or in association with rapid maxillary expansion, as the first step for Class II malocclusion treatment.Methods:The sample comprised 61 patients divided into three groups: Group 1, combined headgear (CH); Group 2, CH + rapid maxillary expansion (CH + RME); and Group 3, control (CG). In Group 1, patients were treated with combined headgear until Class I molar relationship was achieved. In Group 2, the protocol for headgear was the same; however, patients were previously subject to rapid maxillary expansion.Results:Results showed distal displacement of maxillary molars for both experimental groups (p < 0.001), with distal tipping only in Group 1 (CH) (p < 0.001). There was restriction of forward maxillary growth in Group 2 (CH + RME) (p < 0.05) and clockwise rotation of the maxilla in Group 1 (CH) (p < 0.05).Conclusion: Based on the results, it is possible to suggest that treatment w...
Allergologia et Immunopathologia, 2016
Prevalence of respiratory allergic diseases has increased worldwide. Identification of the aeroal... more Prevalence of respiratory allergic diseases has increased worldwide. Identification of the aeroallergens involved in allergenic sensitisation is important for diagnosis, treatment and prevention. To verify the molecular pattern of sensitisation to aeroallergens in patients with allergic respiratory diseases using microarray technique for specific IgE antibody detection. Cross-sectional study of 101 children with allergic rhinitis was followed in an outpatient clinic. All patients had positive skin prick tests (SPT) to at least one of the following antigens: Dermatophagoides pteronyssinus, Blomia tropicalis, Blattella germanica, Lolium multiflorum, and dog and cat epithelium. Serum specific IgE antibodies (sIgE) to mites, animal epithelia, fungi, cockroach and pollens components were determined by ImmunoCAP ISAC. sIgE to group 1 and 2 mite allergens showed higher positive rates: Der p 1 (74.2%), Der p 2 (73.3%), Der f 1 (74.2%), Der f 2 (72.3%). sIgE to animal epithelia were less frequent, Can f 1, Can f 2, Can f 3 in 4.9%, 2.9%, 1.9% respectively and Fel d 1, Fel d 2, Fel d 4 in 16.8%, 0.9% and 1.9%. respectively. Sensitisation to fungi and cockroach were rare, except for Bla g 7, to which 16.8% were positive. There was no significant recognition for tree pollens group. For grass, sIgE were detected to Cyn d 1 in 16.8%, Phl p 1 and Phl p 4 in 14.8% and 12.9%, respectively. Knowing that the pattern of allergic sensitisation varies according to environment and population, our results reinforce the need for local studies, using molecular-based diagnosis.
Revista da Escola de Enfermagem da USP, 2014
This study discusses ethical issues in research involving human beings and seeks to understand th... more This study discusses ethical issues in research involving human beings and seeks to understand the relationship between qualitative research and the ethical care guidelines for Integrative Community Therapy (ICT) circles based on Resolution 466/12 of the National Health Council of the Ministry of Health of Brazil. This is documentary research, which analyzed Resolution 466/12 and ICT circles seeking to make a connection between the ethical guidelines contained in both. The analysis of the corpus was directed toward the construction of the following results: the person's perception, cultural diversity and community. It also brings in consideration of the influence of the ethical dimension of the ICT circles on qualitative research. We conclude that ICT circles are innovative in the sense of the diversity of participants and respect for cultural and social differences. Thus, ICT circles promote acquisition of quality information for social research as well as compliance with the e...
Revista da Escola de Enfermagem da USP, 2014
Comprehensive approach study aimed understanding the reflections and contrasts between personal t... more Comprehensive approach study aimed understanding the reflections and contrasts between personal time and medical therapy protocol time in the life of a young woman with breast cancer. Addressed as a situational study and grounded in Beth’s life story about getting sick and dying of cancer at age 34, the study’s data collection process employed interviews, observation and medical record analysis. The construction of the analytic-synthetic box based on the chronology of Beth’s clinical progression, treatment phases and temporal perception of occurrences enabled us to point out a linear medical therapy protocol time identified by the diagnosis and treatment sequencing process. On the other hand, Beth’s experienced time was marked by simultaneous and non-linear events that generated suffering resulting from the disease. Such comprehension highlights the need for healthcare professionals to take into account the time experienced by the patient, thus providing an indispensable cancer ther...
Texto & Contexto - Enfermagem, 2009
Os Sistemas de Informação em Saúde, tem sido utilizados como ferramentas capazes de armazenar a p... more Os Sistemas de Informação em Saúde, tem sido utilizados como ferramentas capazes de armazenar a produção de dados para subsidiar a avaliação em saúde. Indagamos como esses dados são produzidos e analisados e como o usuário é visualizado no sistema. Trata-se de um Estudo de Caso que possibilitou analisar a configuração da rede de informação em saúde a partir do desenho analítico sobre a produção, fluxo e análise dos dados nos diferentes níveis de atenção à saúde em relação ao Sistema de Informação da Atenção Básica e Sistema de Cadastro Nacional de Portadores de Hipertensão e Diabetes. Evidenciamos que os dados que compõem esses Sistemas de Informação em Saúde não estão sendo analisados, consequentemente, não permitem a análise situacional em saúde e/ou produção de informações para o planejamento da assistência e resolutividade das ações de saúde.
Enfermería Clínica, 2003
Page 1. Resumen La administración de fármacos antineoplásicos en ciclos repetidos por vía intrave... more Page 1. Resumen La administración de fármacos antineoplásicos en ciclos repetidos por vía intravenosa es uno de los pilares fundamentales en el tratamiento del enfermo oncológico. Las diferencias entre el manejo de estas ...
Jornal de Pediatria, 2014
Objective: this study aimed to prepare a silkworm moth (Bombyx mori) antigenic extract and to per... more Objective: this study aimed to prepare a silkworm moth (Bombyx mori) antigenic extract and to perform skin prick tests with this extract in patients with allergic respiratory diseases; to evaluate serum specific immunoglobulin E (IgE) to Bombyx mori using ImmunoCAP® system and to report the frequency of positivity between the two methods and with clinical data. Methods: this was a cross-sectional study with 99 children and adolescents diagnosed with asthma and/or allergic rhinitis, who had skin reactivity to at least one of the six aeroallergens tested. Clinical data were evaluated: skin prick tests with Bombyx mori in-house extract, and total and specific IgE analysis using ImmunoCAP® were performed. Results: the frequency of Bombyx mori specific IgE was found to be 52.5% and 60% using the skin prick test and ImmunoCAP®, respectively. An association between a positive skin test for Bombyx mori and the presence of allergic rhinitis, atopic dermatitis, and urticaria was observed, but the same was not true for asthma or allergic conjunctivitis. There was no relation with the severity of asthma or rhinitis symptoms. Conclusions: a high frequency of sensitization to Bombyx mori was observed in a selected population of patients with respiratory allergic diseases in the city of Curitiba, state of Paraná, Brazil. The extract prepared from the wings of this moth species is effective in demonstrating this sensitivity.
Revista Conectividad
Contextualizar históricamente los espacios museales ha sido un reto debido a la poca información ... more Contextualizar históricamente los espacios museales ha sido un reto debido a la poca información que se ha podido investigar sobre la constitución de algunos museos en el Ecuador, si algo se ha podido obtener es sobre el primer Museo Nacional que estuvo ubicado en la ciudad de Quito desde 1839. A raíz de los procesos independentistas que se empezaron a visibilizar en la ciudad de Guayaquil y debido a un importante auge comercial de muchos productos que empezaron a ingresar por el puerto, se crea el Museo Industrial representado por objetos representativos de la nación y de sus habitantes poco a poco se va fortaleciendo y enriqueciendo y es así que en el 1908 pasa a constituirse como Museo Municipal de Guayaquil cuyo objetivo fue mostrar las reliquias y memorias de nuestros antepasados. A través de esta investigación se pretende reflexionar sobre instituciones que busquen crear un sentimiento de identidad que permita mantener el legado patrimonial como evidencia de identidad territor...
Staphylococcus aureus es el patogeno causante de mastitis bovina con mayor prevalencia en nuestro... more Staphylococcus aureus es el patogeno causante de mastitis bovina con mayor prevalencia en nuestro pais, y suele vincularse a brotes de enfermedades transmitidas por los alimentos. Este patogeno cobra protagonismo por su capacidad de resistir a la terapia antibiotica y producir infecciones en humanos. La perdida de sensibilidad frente a los antimicrobianos es causa de preocupacion a nivel mundial, por lo cual el estudio de la antibioticorresistencia en microorganismos aislados de animales vinculados a la produccion de alimentos es fundamental para la vigilancia epidemiologica. Para realizar el estudio de fenotipos de resistencia, la tecnica de difusion en agar es la mas utilizada y el uso de tecnicas cuantitativas, como la determinacion de la concentracion inhibitoria minima (CIM) y la concentracion bactericida minima (CBM), aportan datos valiosos. El objetivo general es evaluar la susceptibilidad in vitro de cepas de S. aureus aisladas de muestras de leche de vacas provenientes de t...
La evaluacion de la condicion corporal (CC) en vacas es un indicador subjetivo de la cantidad de ... more La evaluacion de la condicion corporal (CC) en vacas es un indicador subjetivo de la cantidad de reservas energeticas del animal y uno de los mejores indicadores del estado nutricional. El monitoreo de la CC permite prever la produccion de leche y la eficiencia reproductiva, evaluar el estado nutricional y reducir la incidencia de enfermedades metabolicas. Uno de los metodos mas utilizados para medir la CC es la forma visual, con la utilizacion de una escala de 1 a 5. En los ultimos anos, han aparecido nuevas tecnologias que podrian reemplazar a la medicion visual y, en esta linea, la empresa DeLaval desarrollo una camara infrarroja que a traves del registro de imagenes 3D de la parte posterior del cuerpo de las vacas calcula un “indice de condicion corporal BCS”. Se trata de un metodo sistematico de estimacion de CC, y es una herramienta de gestion facil de usar. La enfermedad que mayores perdidas economicas genera en los establecimientos lecheros y en la industria lactea es la mas...
La tesi doctoral titulada "Auto-assemblatge de copolimers de bloc per modificacio quimica de... more La tesi doctoral titulada "Auto-assemblatge de copolimers de bloc per modificacio quimica de la superficie", presenta com a objectiu principal el desenvolupament, implementacio i caracteritzacio d'un metode de guiatge de copolimers de bloc basat en la modificacio quimica de la superficie. El desenvolupament d'aquest metode de nanofabricacio contribueix a la futura generacio de dispositius i circuits nanoelectronics. Primer de tot, es presenten els aspectes generals sobre l'auto-assemblatge dirigit de copolimers de bloc, aixi com el seu rol dins del futur de la nanoelectronica comparat amb altres tecnologies emergents. Despres, per tal d'entendre i determinar les interaccions que tenen lloc durant el proces d'auto-assemblatge, es dona una visio general sobre els processos quimics i fisics que tenen lloc en les pel·licules primes de copolimers de bloc. La part principal de la tesi es focalitza en l'estudi, desenvolupament i implementacio d'un meto...
Bovine mastitis is the infectious disease with the greatest impact on world dairy production. In ... more Bovine mastitis is the infectious disease with the greatest impact on world dairy production. In Argentina, Staphylococcus aureus (S. aureus) is the most important pathogen in terms of prevalence and pathogenicity. It can resist antimicrobial treatments through different mechanisms. Currently, there is great concern about antibiotic resistance, both in human medicine and animal health. In our country, there is scarce information about antimicrobial resistance on bovine mastitis strains. The main objective of the project was to evaluate the in Vitro susceptibility of S. aureus isolated from milk samples obtained in dairy farms located in Buenos Aires. Between 2015 and 2016, 109 isolates of milk samples were obtained from cows with clinical mastitis, subclinical and bulk tank milk samples. Until July 2016, the susceptibility of 44 isolates were evaluated following the recommendations of the Clinical and Laboratory Standards Institute (CLSI). The antimicrobial agents included in the st...
Allergologia et Immunopathologia, 2020
Please cite this article in press as: Araujo LML, et al. Identification of the major allergenic p... more Please cite this article in press as: Araujo LML, et al. Identification of the major allergenic proteins from silkworm moth (Bombyx mori) involved in respiratory allergic diseases. Allergol Immunopathol (Madr). 2020.
Resumos do..., 2019
Colorectal cancer (CRC) is the second most common malignant neoplasm in women and the third most ... more Colorectal cancer (CRC) is the second most common malignant neoplasm in women and the third most prevalent in men worldwide. In Brazil, about 36,000 new cases of CRC are estimated among men and women for 2018-2019. With advances in treatment, early CRC is being "reclassified" from a deadly disease to an illness that has great chance of cure. However, so far, the survivors who have completed treatment and are cancer-free, frequently suffer from late/longterm side effects, and some patients with metastasis have poor response to the therapies. In this context, the discovery of new therapeutic strategies is urgently needed. In this project we aimed to validate the hypothesis that 3-Bromopyruvate (3-BP), an alkylating agent, would be able to sensitize the human CRC cell lines to treatment with Vemurafenib, a BRAF inhibitor. Therefore, human CRC cell lines (HT-29 and HCT-116) were challenged with 3-BP followed by the treatment with Vemurafenib. Both cell lines were more responsive to Vemurafenib after sensitization with 3-BP (IC50 values were 7-and 1.4-fold lower than with Vemurafenib alone, for HT-29 and HCT-116 cells, respectively). The findings presented here, highlight the potential of 3-BP in increasing the response of metastatic cancer cells to Vemurafenib.
Animals, 2019
Body condition scoring (BCS) is the management practice of assessing body reserves of individual ... more Body condition scoring (BCS) is the management practice of assessing body reserves of individual animals by visual or tactile estimation of subcutaneous fat and muscle. Both high and low BCS can negatively impact milk production, disease, and reproduction. Visual or tactile estimation of subcutaneous fat reserves in dairy cattle relies on their body shape or thickness of fat layers and muscle on key areas of the body. Although manual BCS has proven beneficial, consistent qualitative scoring can be difficult to implement. The desirable BCS range for dairy cows varies within lactation and should be monitored at multiple time points throughout lactation for the most impact, a practice that can be hard to implement. However, a commercial automatic BCS camera is currently available for dairy cattle (DeLaval Body Condition Scoring, BCS DeLaval International AB, Tumba, Sweden). The objective of this study was to validate the implementation of an automated BCS system in a commercial setting...
Biophysical Journal, 2018
Advances in Patterning Materials and Processes XXXIV, 2017
Directed self-assembly of block copolymers is considered one of the candidates to fulfill the req... more Directed self-assembly of block copolymers is considered one of the candidates to fulfill the requirements of the next technological nodes [1,2]. Polymer domains are aligned by using a chemical and/or a topographical pre-pattern in which preferential surfaces to one of the two blocks or neutral wetting areas are created. In particular, polystyrene-block-polymetylmethacrylate (PS-b-PMMA) has been extensively studied during the last years showing strong capabilities to define periodic nanostructures. However, the relatively low Flory-Huggins parameter and, the resulting low segregation strength of PS-b-PMMA systems, limit their achievable resolution to around 11 nm [3]. The application of block copolymer on sub-10 nm technologies requires the development of the new block copolymer generation known as high- block copolymers. Additionally, an important requirement for their integration on the lithography roadmap is the capability of selectively remove one of the two blocks. The etch contrast between the two domains is typically low due to their organic chemistry. In this sense, selective sequential infiltration synthesis by ALD into one of the blocks can be used in order to incorporate an inorganic material. The formed organic/inorganic blend sustains better the plasma etching to remove the non-infiltrated organic block. In this contribution, we show the use of high- polystyrene-b-polylactide acid (PS-b-PLA) lamellar block copolymer for line/space applications. PS-b-PLA has a larger Flory-Huggins parameter (=0.218 at room temperature[4]) compared with PS-b-PMMA, allowing the size reduction of the self-assembled domains. The method to control the orientation of the polymer domains is similar to the one typically used for PS-b-PMMA. Chemical contrast and the subsequent alignment of the polymer domains are achieved by the definition of a chemical pre-pattern on a random copolymer PS-r-PMMA (48% of PS) (figure 1). The polymer brush is grafted on the substrate and then locally modified by the combination of e-beam lithography and soft oxygen plasma. Afterwards, the PS-b-PLA block copolymer is spin-coated and thermally annealed on the chemically pre-patterned substrate. A chemical contrast is observed between the modified and unmodified stripes. While, the lamellar domains are oriented perpendicular to the substrate on unmodified areas, PLA domains are strongly attracted to the O2 modified surfaces inducing a parallel orientation to the substrate. Additionally, the wetting behavior of the polymer domains is also studied through the difference of surface free energy between the substrate and each polymer block. The energy estimated by the Young´s equation [Δγ =γSA -γSB= γAB·cos(ØAB)], where γSA and γSB are the interface tensions between homo-polymers A and B with the substrate, and ØAB is the contact angle between A and B homo-polymers which is obtained in de-wetting experiments. Finally, sequential infiltration synthesis is used to selectively infiltrate alumina (Al3O2) on PLA domains (figure 2). A selective infiltration is achieved because the precursor molecules react with the carbonyl (C=O) groups that are only present in the PLA block. After five cycles of SIS, the SIS modified PLA domains become more resistant to O2 plasma etching than PS enabling the PS etching without using other kind of hard-masks. The research leading to these results received funding from the European Union’s Seventh Framework Program FP7/2007-2013, under the project CoLiSA and the European Nanoelectronics Initiative Advisory Council under the project PLACYD (ENIAC-2013-2). L. Evangelio acknowledges MECD for the PhD contract FPU13/03746 [1] R. Ruiz, H. Kang, F. A. Detcheverry, E. Dobisz, D. S. Kercher, T. R. Albrecht, J. J. de Pablo, P. F. Nealey, Science 321 (2008) 936-939. [2] M. Fernández-Regúlez, L, Evangelio, M. Lorenzoni, J. Fraxedas, F. Perez-Murano, ACS Appl. Mater. Interfaces 6 (2014) 21596-21602. [3] L. Wan, R. Ruiz, H. Gao, K. C. Patel, T. R. Albrecht, J. Yin, J. Kim, Y. Cao, G. Lin, ACS Nano 9 (2015) 7506-7514 [4] A. S. Zalusky, R. Olayo-Valles , J. H. Wolf , M. A. Hillmyer, J. Am. Chem. Soc. 124 (2002) 12761-12773
Revista da Escola de Enfermagem da USP, 2016
An essay that aims to reflect on the family experience of care in chronic situation, increasing t... more An essay that aims to reflect on the family experience of care in chronic situation, increasing the understanding of the family as the primary caregiver. It is based on comprehensive approach in studies conducted in three matrix searches from family care experiences. We have taken three axes to organize our reflections: a) conformation of family care in chronic situation, highlighting the multiple costs incurred to the family, which can exhaust the potential of care and establish or increase its vulnerability if it is not backed by networks support and sustenance; b) family rearrangements for the care, giving visibility to care cores in which many loved family members share the care, dynamic, plural and changeable way; c) self care modeling family care, pointing to the range of possibilities of the person taking care of diseased conditions supported by people close to them. We learn that the family takes care of itself in everyday life and in the illness experience, creating network...
Anuario De Investigacion Usal, Sep 24, 2014
La leche de alta calidad debe poseer una composición adecuada, estar libre de patógenos, sustanci... more La leche de alta calidad debe poseer una composición adecuada, estar libre de patógenos, sustancias extrañas, olores, sedimentos y debe tener un contenido bajo de bacterias contaminantes y células somáticas. El consumo de lácteos elaborados a partir de leche de baja calidad impacta negativamente en la salud de los consumidores (Errecalde,1996; Giesecke, 1983; & Heeschen et al.1985). Por el contrario, producir leche de buena calidad es un factor que estabiliza el desarrollo socio-económico en áreas rurales, particularmente en los países en desarrollo (De Boer, 1981; & Mc Dowell, 1981). Argentina es el segundo productor latinoamericano de leche cruda y es el onceavo a nivel mundial pero la producción primaria se encuentra dividida en dos grupos: uno de alta y otro de baja eficiencia. Según el INTA y en MAGyP, el mejoramiento de la calidad de leche es un punto crítico que los productores de menor eficiencia deben resolver para cumplir con la creciente demanda que se espera en el mundo (INTA-MAGYP, 2011). En nuestro país existen numerosos trabajos que caracterizan la calidad de la leche pero no hay
Enfermeria Cientifica, 2004
Dental Press Journal of Orthodontics, 2015
Objective: The aim of this study was to assess the effects of combined headgear used alone or in ... more Objective: The aim of this study was to assess the effects of combined headgear used alone or in association with rapid maxillary expansion, as the first step for Class II malocclusion treatment.Methods:The sample comprised 61 patients divided into three groups: Group 1, combined headgear (CH); Group 2, CH + rapid maxillary expansion (CH + RME); and Group 3, control (CG). In Group 1, patients were treated with combined headgear until Class I molar relationship was achieved. In Group 2, the protocol for headgear was the same; however, patients were previously subject to rapid maxillary expansion.Results:Results showed distal displacement of maxillary molars for both experimental groups (p < 0.001), with distal tipping only in Group 1 (CH) (p < 0.001). There was restriction of forward maxillary growth in Group 2 (CH + RME) (p < 0.05) and clockwise rotation of the maxilla in Group 1 (CH) (p < 0.05).Conclusion: Based on the results, it is possible to suggest that treatment w...
Allergologia et Immunopathologia, 2016
Prevalence of respiratory allergic diseases has increased worldwide. Identification of the aeroal... more Prevalence of respiratory allergic diseases has increased worldwide. Identification of the aeroallergens involved in allergenic sensitisation is important for diagnosis, treatment and prevention. To verify the molecular pattern of sensitisation to aeroallergens in patients with allergic respiratory diseases using microarray technique for specific IgE antibody detection. Cross-sectional study of 101 children with allergic rhinitis was followed in an outpatient clinic. All patients had positive skin prick tests (SPT) to at least one of the following antigens: Dermatophagoides pteronyssinus, Blomia tropicalis, Blattella germanica, Lolium multiflorum, and dog and cat epithelium. Serum specific IgE antibodies (sIgE) to mites, animal epithelia, fungi, cockroach and pollens components were determined by ImmunoCAP ISAC. sIgE to group 1 and 2 mite allergens showed higher positive rates: Der p 1 (74.2%), Der p 2 (73.3%), Der f 1 (74.2%), Der f 2 (72.3%). sIgE to animal epithelia were less frequent, Can f 1, Can f 2, Can f 3 in 4.9%, 2.9%, 1.9% respectively and Fel d 1, Fel d 2, Fel d 4 in 16.8%, 0.9% and 1.9%. respectively. Sensitisation to fungi and cockroach were rare, except for Bla g 7, to which 16.8% were positive. There was no significant recognition for tree pollens group. For grass, sIgE were detected to Cyn d 1 in 16.8%, Phl p 1 and Phl p 4 in 14.8% and 12.9%, respectively. Knowing that the pattern of allergic sensitisation varies according to environment and population, our results reinforce the need for local studies, using molecular-based diagnosis.
Revista da Escola de Enfermagem da USP, 2014
This study discusses ethical issues in research involving human beings and seeks to understand th... more This study discusses ethical issues in research involving human beings and seeks to understand the relationship between qualitative research and the ethical care guidelines for Integrative Community Therapy (ICT) circles based on Resolution 466/12 of the National Health Council of the Ministry of Health of Brazil. This is documentary research, which analyzed Resolution 466/12 and ICT circles seeking to make a connection between the ethical guidelines contained in both. The analysis of the corpus was directed toward the construction of the following results: the person's perception, cultural diversity and community. It also brings in consideration of the influence of the ethical dimension of the ICT circles on qualitative research. We conclude that ICT circles are innovative in the sense of the diversity of participants and respect for cultural and social differences. Thus, ICT circles promote acquisition of quality information for social research as well as compliance with the e...
Revista da Escola de Enfermagem da USP, 2014
Comprehensive approach study aimed understanding the reflections and contrasts between personal t... more Comprehensive approach study aimed understanding the reflections and contrasts between personal time and medical therapy protocol time in the life of a young woman with breast cancer. Addressed as a situational study and grounded in Beth’s life story about getting sick and dying of cancer at age 34, the study’s data collection process employed interviews, observation and medical record analysis. The construction of the analytic-synthetic box based on the chronology of Beth’s clinical progression, treatment phases and temporal perception of occurrences enabled us to point out a linear medical therapy protocol time identified by the diagnosis and treatment sequencing process. On the other hand, Beth’s experienced time was marked by simultaneous and non-linear events that generated suffering resulting from the disease. Such comprehension highlights the need for healthcare professionals to take into account the time experienced by the patient, thus providing an indispensable cancer ther...
Texto & Contexto - Enfermagem, 2009
Os Sistemas de Informação em Saúde, tem sido utilizados como ferramentas capazes de armazenar a p... more Os Sistemas de Informação em Saúde, tem sido utilizados como ferramentas capazes de armazenar a produção de dados para subsidiar a avaliação em saúde. Indagamos como esses dados são produzidos e analisados e como o usuário é visualizado no sistema. Trata-se de um Estudo de Caso que possibilitou analisar a configuração da rede de informação em saúde a partir do desenho analítico sobre a produção, fluxo e análise dos dados nos diferentes níveis de atenção à saúde em relação ao Sistema de Informação da Atenção Básica e Sistema de Cadastro Nacional de Portadores de Hipertensão e Diabetes. Evidenciamos que os dados que compõem esses Sistemas de Informação em Saúde não estão sendo analisados, consequentemente, não permitem a análise situacional em saúde e/ou produção de informações para o planejamento da assistência e resolutividade das ações de saúde.
Enfermería Clínica, 2003
Page 1. Resumen La administración de fármacos antineoplásicos en ciclos repetidos por vía intrave... more Page 1. Resumen La administración de fármacos antineoplásicos en ciclos repetidos por vía intravenosa es uno de los pilares fundamentales en el tratamiento del enfermo oncológico. Las diferencias entre el manejo de estas ...
Jornal de Pediatria, 2014
Objective: this study aimed to prepare a silkworm moth (Bombyx mori) antigenic extract and to per... more Objective: this study aimed to prepare a silkworm moth (Bombyx mori) antigenic extract and to perform skin prick tests with this extract in patients with allergic respiratory diseases; to evaluate serum specific immunoglobulin E (IgE) to Bombyx mori using ImmunoCAP® system and to report the frequency of positivity between the two methods and with clinical data. Methods: this was a cross-sectional study with 99 children and adolescents diagnosed with asthma and/or allergic rhinitis, who had skin reactivity to at least one of the six aeroallergens tested. Clinical data were evaluated: skin prick tests with Bombyx mori in-house extract, and total and specific IgE analysis using ImmunoCAP® were performed. Results: the frequency of Bombyx mori specific IgE was found to be 52.5% and 60% using the skin prick test and ImmunoCAP®, respectively. An association between a positive skin test for Bombyx mori and the presence of allergic rhinitis, atopic dermatitis, and urticaria was observed, but the same was not true for asthma or allergic conjunctivitis. There was no relation with the severity of asthma or rhinitis symptoms. Conclusions: a high frequency of sensitization to Bombyx mori was observed in a selected population of patients with respiratory allergic diseases in the city of Curitiba, state of Paraná, Brazil. The extract prepared from the wings of this moth species is effective in demonstrating this sensitivity.