Laura Soares Veloso - Academia.edu (original) (raw)

Papers by Laura Soares Veloso

Research paper thumbnail of Evolução dos inventários nacionais de gases de efeito estufa para o setor de uso da terra, mudança de uso da terra e florestas

Desde sua entrada na Convencao, o Brasil submeteu tres Inventarios nacionais de GEE. Nesses docum... more Desde sua entrada na Convencao, o Brasil submeteu tres Inventarios nacionais de GEE. Nesses documentos e significativa a contribuicao do setor Uso da terra, Mudanca do Uso da Terra e Florestas (LULUCF) nas emissoes totais. Por ser este um setor-chave para o pais, o presente trabalho teve como objetivo principal compreender a evolucao das metodologias de estimativas das emissoes do setor LULUCF nos Inventarios de GEE do Brasil, bem como analisar comparativamente as origens e implicacoes das alteracoes metodologicas entre o Segundo e Terceiro Inventario na estimativa das emissoes para 2005. A analise desse ano e particularmente importante, pois a contribuicao nacionalmente determinada (NDC) do pais foi calculada com base em 2005, e o Terceiro Inventario apresenta uma emissao liquida total 25% superior ao mesmo ano de reporte do Segundo Inventario. Visto que ainda existe um debate, seja dentro do pais como tambem na UNFCCC, sobre como as mudancas metodologicas das comunicacoes devem se...

Research paper thumbnail of A defective TLR4 signaling for IFN-β expression is responsible for the innately lower ability of BALB/c macrophages to produce NO in response to LPS as compared to C57BL/6

PloS one, 2014

C57BL/6 mice macrophages innately produce higher levels of NO than BALB/c cells when stimulated w... more C57BL/6 mice macrophages innately produce higher levels of NO than BALB/c cells when stimulated with LPS. Here, we investigated the molecular events that account for this intrinsic differential production of NO. We found that the lower production of NO in BALB/c is not due to a subtraction of L-arginine by arginase, and correlates with a lower iNOS accumulation, which is independent of its degradation rate. Instead, the lower accumulation of iNOS is due to the lower levels of iNOS mRNA, previously shown to be also independent of its stability, suggesting that iNOS transcription is less efficient in BALB/c than in C57BL/6 macrophages. Activation of NFκB is more efficient in BALB/c, thus not correlating with iNOS expression. Conversely, activation of STAT-1 does correlate with iNOS expression, being more prominent in C57BL/6 than in BALB/c macrophages. IFN-β and IL-10 are more highly expressed in C57BL/6 than in BALB/c macrophages, and the opposite is true for TNF-α. Whereas IL-10 and...

Research paper thumbnail of The Rights and Wrongs of Brazil's Forest Monitoring System

Conservation Letters, 2017

Research paper thumbnail of A Defective TLR4 Signaling for IFN-b Expression Is Responsible for the Innately Lower Ability of BALB/c Macrophages to Produce NO in Response to LPS as Compared to C57BL/6

C57BL/6 mice macrophages innately produce higher levels of NO than BALB/c cells when stimulated w... more C57BL/6 mice macrophages innately produce higher levels of NO than BALB/c cells when stimulated with LPS. Here, we investigated the molecular events that account for this intrinsic differential production of NO. We found that the lower production of NO in BALB/c is not due to a subtraction of L-arginine by arginase, and correlates with a lower iNOS accumulation, which is independent of its degradation rate. Instead, the lower accumulation of iNOS is due to the lower levels of iNOS mRNA, previously shown to be also independent of its stability, suggesting that iNOS transcription is less efficient in BALB/c than in C57BL/6 macrophages. Activation of NFkB is more efficient in BALB/c, thus not correlating with iNOS expression. Conversely, activation of STAT-1 does correlate with iNOS expression, being more prominent in C57BL/6 than in BALB/c macrophages. IFN-b and IL-10 are more highly expressed in C57BL/6 than in BALB/c macrophages, and the opposite is true for TNF-a. Whereas IL-10 and TNF-a do not seem to participate in their differential production of NO, IFN-b has a determinant role since 1) anti-IFN-b neutralizing antibodies abolish STAT-1 activation reducing NO production in C57BL/6 macrophages to levels as low as in BALB/c cells and 2) exogenous rIFN-b confers to LPS-stimulated BALB/c macrophages the ability to phosphorylate STAT-1 and to produce NO as efficiently as C57BL/6 cells. We demonstrate, for the first time, that BALB/c macrophages are innately lower NO producers than C57BL/6 cells because they are defective in the TLR-4-induced IFN-b-mediated STAT-1 activation pathway.

Research paper thumbnail of The Rights and Wrongs of Brazil's Forest Monitoring Systems

Research paper thumbnail of Evolução dos inventários nacionais de gases de efeito estufa para o setor de uso da terra, mudança de uso da terra e florestas

Desde sua entrada na Convencao, o Brasil submeteu tres Inventarios nacionais de GEE. Nesses docum... more Desde sua entrada na Convencao, o Brasil submeteu tres Inventarios nacionais de GEE. Nesses documentos e significativa a contribuicao do setor Uso da terra, Mudanca do Uso da Terra e Florestas (LULUCF) nas emissoes totais. Por ser este um setor-chave para o pais, o presente trabalho teve como objetivo principal compreender a evolucao das metodologias de estimativas das emissoes do setor LULUCF nos Inventarios de GEE do Brasil, bem como analisar comparativamente as origens e implicacoes das alteracoes metodologicas entre o Segundo e Terceiro Inventario na estimativa das emissoes para 2005. A analise desse ano e particularmente importante, pois a contribuicao nacionalmente determinada (NDC) do pais foi calculada com base em 2005, e o Terceiro Inventario apresenta uma emissao liquida total 25% superior ao mesmo ano de reporte do Segundo Inventario. Visto que ainda existe um debate, seja dentro do pais como tambem na UNFCCC, sobre como as mudancas metodologicas das comunicacoes devem se...

Research paper thumbnail of A defective TLR4 signaling for IFN-β expression is responsible for the innately lower ability of BALB/c macrophages to produce NO in response to LPS as compared to C57BL/6

PloS one, 2014

C57BL/6 mice macrophages innately produce higher levels of NO than BALB/c cells when stimulated w... more C57BL/6 mice macrophages innately produce higher levels of NO than BALB/c cells when stimulated with LPS. Here, we investigated the molecular events that account for this intrinsic differential production of NO. We found that the lower production of NO in BALB/c is not due to a subtraction of L-arginine by arginase, and correlates with a lower iNOS accumulation, which is independent of its degradation rate. Instead, the lower accumulation of iNOS is due to the lower levels of iNOS mRNA, previously shown to be also independent of its stability, suggesting that iNOS transcription is less efficient in BALB/c than in C57BL/6 macrophages. Activation of NFκB is more efficient in BALB/c, thus not correlating with iNOS expression. Conversely, activation of STAT-1 does correlate with iNOS expression, being more prominent in C57BL/6 than in BALB/c macrophages. IFN-β and IL-10 are more highly expressed in C57BL/6 than in BALB/c macrophages, and the opposite is true for TNF-α. Whereas IL-10 and...

Research paper thumbnail of The Rights and Wrongs of Brazil's Forest Monitoring System

Conservation Letters, 2017

Research paper thumbnail of A Defective TLR4 Signaling for IFN-b Expression Is Responsible for the Innately Lower Ability of BALB/c Macrophages to Produce NO in Response to LPS as Compared to C57BL/6

C57BL/6 mice macrophages innately produce higher levels of NO than BALB/c cells when stimulated w... more C57BL/6 mice macrophages innately produce higher levels of NO than BALB/c cells when stimulated with LPS. Here, we investigated the molecular events that account for this intrinsic differential production of NO. We found that the lower production of NO in BALB/c is not due to a subtraction of L-arginine by arginase, and correlates with a lower iNOS accumulation, which is independent of its degradation rate. Instead, the lower accumulation of iNOS is due to the lower levels of iNOS mRNA, previously shown to be also independent of its stability, suggesting that iNOS transcription is less efficient in BALB/c than in C57BL/6 macrophages. Activation of NFkB is more efficient in BALB/c, thus not correlating with iNOS expression. Conversely, activation of STAT-1 does correlate with iNOS expression, being more prominent in C57BL/6 than in BALB/c macrophages. IFN-b and IL-10 are more highly expressed in C57BL/6 than in BALB/c macrophages, and the opposite is true for TNF-a. Whereas IL-10 and TNF-a do not seem to participate in their differential production of NO, IFN-b has a determinant role since 1) anti-IFN-b neutralizing antibodies abolish STAT-1 activation reducing NO production in C57BL/6 macrophages to levels as low as in BALB/c cells and 2) exogenous rIFN-b confers to LPS-stimulated BALB/c macrophages the ability to phosphorylate STAT-1 and to produce NO as efficiently as C57BL/6 cells. We demonstrate, for the first time, that BALB/c macrophages are innately lower NO producers than C57BL/6 cells because they are defective in the TLR-4-induced IFN-b-mediated STAT-1 activation pathway.

Research paper thumbnail of The Rights and Wrongs of Brazil's Forest Monitoring Systems