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Papers by Lauren Lichten

Research paper thumbnail of The zebrafish gene claudinj is essential for normal ear function and important for the formation of the otoliths

Mechanisms of Development, 2005

We have identified a mutation in the zebrafish gene claudinj generated by retroviral integration.... more We have identified a mutation in the zebrafish gene claudinj generated by retroviral integration. Mutant embryos display otoliths severely reduced in size, no response to tapping stimulus, and an inability to balance properly suggesting vestibular and hearing dysfunction. Antisense in situ hybridization to the cldnj gene showed expression first in the otic placode and later asymmetric expression in the otic vesicle. Morpholino inhibition of claudinj expression showed similar defects in otolith formation. Phylogenetic analysis of claudin sequences from multiple species demonstrates that claudinj was part of a gene expansion that began in the common ancestor of fish and humans, but additional fish specific gene duplications must have also occurred. Published by Elsevier Ireland Ltd.

Research paper thumbnail of Prenatal genetics: the evolution and future directions of screening and diagnosis

The Journal of perinatal & neonatal nursing

... Neonatal Care. Prenatal Genetics: The Evolution and Future Directions of Screening and Diagno... more ... Neonatal Care. Prenatal Genetics: The Evolution and Future Directions of Screening and Diagnosis. Slack, Carolyn MS; Lurix, Kerry MS; Lewis, Sarah MS; Lichten, Lauren MS. Article Outline. Collapse Box Author Information. Division ...

Research paper thumbnail of Prenatal Genetics

The Journal of Perinatal & Neonatal Nursing, 2006

Research paper thumbnail of The Karyopherin Msn5/Kap142 Requires Nup82 for Nuclear Export and Performs a Function Distinct from Translocation in RPA Protein Import

Journal of Biological Chemistry, 2004

Protein transport between the nucleus and cytoplasm requires interactions between nuclear pore co... more Protein transport between the nucleus and cytoplasm requires interactions between nuclear pore complex proteins (nucleoporins) and soluble nuclear transport factors (karyopherins, importins, and exportins). Exactly how these interactions contribute to the nucleocytoplasmic transport of substrates remains unclear. Using a synthetic lethal screen with the nucleoporin NUP1, we have identified a conditional allele of NUP82, encoding an essential nuclear pore complex protein in Saccharomyces cerevisiae. This nup82-3 allele also exhibits synthetic genetic interactions with mutants of the karyopherin MSN5. nup82-3 mutants accumulate the Msn5 export substrate Pho4 within the nucleus at nonpermissive temperatures. The nuclear import of the RPA complex subunit Rfa2 is impaired in nup82-3 and in mutants of the karyopherin KAP95, but is not affected by the loss of MSN5. Interestingly, deletion of MSN5 results in retention of Rfa2-GFP within the nucleus under conditions in which it normally diffuses out. These data provide evidence that Nup82 is important for Msn5mediated nuclear protein export and Kap95-mediated protein import. In addition, Msn5 may play a role independent of import in the localization of Rfa2.

Research paper thumbnail of The zebrafish gene claudinj is essential for normal ear function and important for the formation of the otoliths

Mechanisms of Development, 2005

We have identified a mutation in the zebrafish gene claudinj generated by retroviral integration.... more We have identified a mutation in the zebrafish gene claudinj generated by retroviral integration. Mutant embryos display otoliths severely reduced in size, no response to tapping stimulus, and an inability to balance properly suggesting vestibular and hearing dysfunction. Antisense in situ hybridization to the cldnj gene showed expression first in the otic placode and later asymmetric expression in the otic vesicle. Morpholino inhibition of claudinj expression showed similar defects in otolith formation. Phylogenetic analysis of claudin sequences from multiple species demonstrates that claudinj was part of a gene expansion that began in the common ancestor of fish and humans, but additional fish specific gene duplications must have also occurred. Published by Elsevier Ireland Ltd.

Research paper thumbnail of Prenatal genetics: the evolution and future directions of screening and diagnosis

The Journal of perinatal & neonatal nursing

... Neonatal Care. Prenatal Genetics: The Evolution and Future Directions of Screening and Diagno... more ... Neonatal Care. Prenatal Genetics: The Evolution and Future Directions of Screening and Diagnosis. Slack, Carolyn MS; Lurix, Kerry MS; Lewis, Sarah MS; Lichten, Lauren MS. Article Outline. Collapse Box Author Information. Division ...

Research paper thumbnail of Prenatal Genetics

The Journal of Perinatal & Neonatal Nursing, 2006

Research paper thumbnail of The Karyopherin Msn5/Kap142 Requires Nup82 for Nuclear Export and Performs a Function Distinct from Translocation in RPA Protein Import

Journal of Biological Chemistry, 2004

Protein transport between the nucleus and cytoplasm requires interactions between nuclear pore co... more Protein transport between the nucleus and cytoplasm requires interactions between nuclear pore complex proteins (nucleoporins) and soluble nuclear transport factors (karyopherins, importins, and exportins). Exactly how these interactions contribute to the nucleocytoplasmic transport of substrates remains unclear. Using a synthetic lethal screen with the nucleoporin NUP1, we have identified a conditional allele of NUP82, encoding an essential nuclear pore complex protein in Saccharomyces cerevisiae. This nup82-3 allele also exhibits synthetic genetic interactions with mutants of the karyopherin MSN5. nup82-3 mutants accumulate the Msn5 export substrate Pho4 within the nucleus at nonpermissive temperatures. The nuclear import of the RPA complex subunit Rfa2 is impaired in nup82-3 and in mutants of the karyopherin KAP95, but is not affected by the loss of MSN5. Interestingly, deletion of MSN5 results in retention of Rfa2-GFP within the nucleus under conditions in which it normally diffuses out. These data provide evidence that Nup82 is important for Msn5mediated nuclear protein export and Kap95-mediated protein import. In addition, Msn5 may play a role independent of import in the localization of Rfa2.

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