Leandra Naíra Zambelli Ramalho - Academia.edu (original) (raw)
Papers by Leandra Naíra Zambelli Ramalho
RESUMO: A deiscência de anastomose é uma das mais graves complicações advindas de operações do tu... more RESUMO: A deiscência de anastomose é uma das mais graves complicações advindas de operações do tubo gastrintestinal. Algumas condições gerais podem prejudicar o processo de cicatrização, tais como: desnutrição e hipoalbuminemia. Este estudo experimental teve como objetivo avaliar a cicatrização de anastomoses colônicas na vigência de desnutrição protéico-calórica e hipoalbuminemia. Dividimos os animais em dois grupos, sendo um deles o controle e o outro desnutrido (ingestão diária de metade da ração do grupo controle por vinte dias). O peso corporal, a albumina sérica, a evolução clínica, a cavidade abdominal, os aspectos macro e microscópicos da anastomose, a esteatose hepática e a concentração tecidual de hidroxiprolina foram observadas em cada animal. Pudemos notar que o método utilizado para desnutrir os animais mostrou-se eficaz, uma vez que houve redução significativa do peso do grupo experimental. Observamos que o grupo desnutrido apresentou dados necroscópicos de pior prognóstico e mortalidade superior ao grupo controle. Concluímos que a desnutrição influencia negativamente na cicatrização de anastomoses colônicas e aumenta significativamente a mortalidade. Descritores: desnutrição, anastomose colônica, cicatrização. FERREIRA MM; SCIALOM JM; CAMPOS AD; RAMALHO LLZ; MARCHINI JS; FÉRES O; ROCHA JJR. Efeito da Desnutrição na Cicatrização de Anastomoses Colônicas: Estudo Experimental em Ratos. Rev bras Coloproct, 2006;26(3): 239-243.
BioMed Research International, 2015
Nonalcoholic fatty liver disease (NAFLD) is characterized by hepatic steatosis, which occurs in t... more Nonalcoholic fatty liver disease (NAFLD) is characterized by hepatic steatosis, which occurs in the absence of alcohol abuse. NAFLD can evolve into progressive liver injury and fibrosis in the form of nonalcoholic steatohepatitis (NASH). Several animal models have been developed to attempt to represent the morphological, biochemical, and clinical features of human NASH. The actual review presents a critical analysis of the most commonly used experimental models of NAFLD/NASH development. These models can be classified into genetic, nutritional, and a combination of genetic and nutritional factors. The main genetic models are ob/ob and db/db mutant mice and Zucker rats. The principal nutritional models employ methionine-and choline-deficient, high-fat, high-cholesterol and high-cholate, cafeteria, and high-fructose diets. Currently, associations between high-fructose and various compositions of high-fat diets have been widely studied. Previous studies have encountered significant difficulties in developing animal models capable of reproducing human NASH. Some models produce consistent morphological findings, but the induction method differs significantly compared with the pathophysiology of human NASH. Other models precisely represent the clinical and etiological contexts of this disease but fail to provide accurate histopathological representations mainly in the progression from steatosis to liver fibrosis.
Archives of pathology & laboratory medicine, 2007
Although there is evidence that endometriosis results from basal endometrium dislocation, the und... more Although there is evidence that endometriosis results from basal endometrium dislocation, the underlying biology is not fully understood. One protein that plays an important role in regulating epithelial proliferation and differentiation is the 63-kDa membrane protein (p63), which is also a marker of basal and reserve cells in the female genital tract. To determine whether p63 is expressed differently in peritoneal endometriosis, endometriomas, and adenomyosis, as well as in deep endometriotic nodules of the rectovaginal septum and abdominal wall. This study includes a prospective series of consecutive patients (Canadian Task Force classification II-2) from a tertiary care university hospital. Specimens collected from 83 patients (15 peritoneal endometriosis specimens, 22 endometrioma specimens, 36 adenomyosis specimens, and 10 rectovaginal septum/abdominal wall specimens) were evaluated. Diagnostic and operative laparoscopies or laparotomies were performed, and tissue samples were ...
Journal of photochemistry and photobiology. B, Biology, 2015
Nitric oxide (NO) levels increase considerably after 24h of exposure of skin to ultraviolet B (UV... more Nitric oxide (NO) levels increase considerably after 24h of exposure of skin to ultraviolet B (UVB) radiation, which leads to nitrosative skin injury. In addition, increased NO levels after exposure to UVB radiation are associated with inhibition of cell proliferation. Compared to the UV-control group, UV-genistein at 10mg/kg (UV-GEN10) group showed tissue protection, decreased lipid peroxide and nitrotyrosine formation, and low CAT activity. Furthermore, NO levels and iNOS labeling remained high. In this group, the reduction in lipid peroxides and nitrotyrosine was accompanied by upregulation of cell proliferation factors (Ki67 and PCNA), which indicated that prevention of nitrosative skin injury promoted cell proliferation and DNA repair. Genistein also prevented nitrosative events, inhibited ONOO(-) formation, which leads to tissue protection and cell proliferation. The UV-GEN15 group did not result in a greater protective effect compared to that with UV-GEN10 group. In the UV-GE...
Acta Cirurgica Brasileira, 2001
ABSTRACT
Acta Cirurgica Brasileira, 2001
ABSTRACT
Acta Cirurgica Brasileira, 2001
ABSTRACT
Genetics and molecular research : GMR, 2005
Hereditary hemochromatosis (HH) is the most common genetic disease among individuals of European ... more Hereditary hemochromatosis (HH) is the most common genetic disease among individuals of European descent. Two mutations (845G-->A, C282Y and 187C-->G, H63D) in the hemochromatosis gene (HFE gene) are associated with HH. About 85-90% of patients of northern European descent with HH are C282Y homozygous. The prevalence of HH in the Brazilian population, which has a very high level of racial admixture, is unknown. The aims of the present study were to identify individuals with diagnostic criteria for HH among patients with a body iron overload attended at the university hospital of the Faculty of Medicine of Ribeirao Preto from 1990 to 2000, and to evaluate the prevalence of HFE mutations. We screened first-degree relatives for HFE mutations. Four of 72 patients (three men and one woman, mean age 47 years) fulfilled the criteria for HH. HFE mutations were studied in three patients [two C282Y homozygotes (patients 1 and 2) and one H63D heterozygote]. Patient 1 had four children (a...
Molecular Medicine Reports, 2008
This study aims to investigate human papilloma virus (HPV) frequency in Brazilian patients with o... more This study aims to investigate human papilloma virus (HPV) frequency in Brazilian patients with oral squamous cell carcinoma (OSCC) in order to establish a clinicopathological profile. It will also examine the correlation between patient survival and HPV expression in primary tumors (PTs), and their matched samples (MSs) of recidives, lymph nodal metastasis (LNM) or necropsies. Eighty-seven PTs and their corresponding 87 MSs were tested for HPV infection by polymerase chain reaction (PCR) using general and type-specific HPV primers. The following data were obtained from patient medical files: primary site, age, gender, tobacco consumption, histological differentiation, recurrences, metastasis, disease-free survival (DFS) and overall survival (OS). Of the 87 patients investigated, 17 (19.5%) were found to have HPV DNA in their tumors. An investigation of all the paraffin-embedded specimens revealed the presence of HPV DNA in 18 of the 174 samples (10.4%), 10 (11.5%) from PTs and 8 (9.2%) from MSs. No virus was detected in the corresponding PT of 7 (8.1%) MSs, and only one patient demonstrated HPV DNA positivity in both samples. The HPV genotypes 16 and 18 were detected in 4 (22.2%) and 3 (16.7%) of the positive samples, respectively. Infection with both genotypes was found in 6 (33.3%) investigated samples, and the HPV genotype was unidentified in 5 (27.8%) samples. The tongue was the most prevalent infected anatomical site. We did not find any significant association between HPV infection and gender, age, histological differentiation, DFS or OS. A significant number of HPV samples were positive among non-smoking patients. Although a possible influence of the virus on tumoral induction cannot be ruled out, the low frequency of OSCC cases that contain HPV does not suggest that this virus has the same etiological influence on patients as tobacco consumption does.
![Research paper thumbnail of [617] H63D MUTATION IN HFE GENE: ANOTHER INDEPENDENT FACTOR OF SUSTAINED VIROLOGICAL RESPONSE TO INTERFERON AND RIBAVIRIN IN PATIENTS WITH CHRONIC HEPATITIS C INFECTION (HCV)?](https://attachments.academia-assets.com/44871184/thumbnails/1.jpg)
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Statins exert beneficial effects in chronically damaged tissues. Angiotensin II (ANG II) particip... more Statins exert beneficial effects in chronically damaged tissues. Angiotensin II (ANG II) participates in liver fibrogenesis by inducing oxidative stress, inflammation, and transforming growth factor-beta1 (TGF-beta1) expression. We investigate whether atorvastatin modulates ANG II-induced pathogenic effects in the liver. Male Wistar rats were infused with saline or ANG II (100 ng kg(-1) min(-1)) for 4 wk through a subcutaneous osmotic pump. Rats received either vehicle or atorvastatin (5 mg kg(-1) day(-1)) by gavage. ANG II infusion resulted in infiltration of inflammatory cells (CD43 immunostaining), oxidative stress (4-hydroxynonenal), hepatic stellate cells (HSC) activation (smooth muscle alpha-actin), increased intercellular adhesion molecule (ICAM-1), and interleukin-6 hepatic gene expression (quantitative PCR). These effects were markedly blunted in rats receiving atorvastatin. The beneficial effects of atorvastatin were confirmed in an additional model of acute liver injury (carbon tetrachloride administration). We next explored whether the beneficial effects of atorvastatin on ANG II-induced actions are also reproduced at the cellular level. We studied HSC, a cell type with inflammatory and fibrogenic properties. ANG II (10(-8)M) stimulated cell proliferation, proinflammatory actions (NF-kappaB activation, ICAM-1 expression, interleukin-8 secretion) as well as expression of procollagen-alpha(1(I)) and TGF-beta1. All of these effects were reduced in the presence of atorvastatin (10(-7)M). These results indicate that atorvastatin attenuates the pathogenic events induced by ANG II in the liver both in vivo and in vitro. Therefore, statins could have beneficial effects in conditions characterized by hepatic inflammation.
ABSTRACT The inhibition of the renin-angiotensin system (RAS) display anti-fibrogenic effects in ... more ABSTRACT The inhibition of the renin-angiotensin system (RAS) display anti-fibrogenic effects in liver cirrhosis, however RAS influence on schistosomal fibrosis has not been totally clarified. Thus, our objective was to compare the activities of different antihypertensive drugs on the inhibition of the RAS in murine schistosomal fibrosis. BALB/c mice (n=40) weighing 20g were subjected to inoculation of 50 cercariae and subdivided in three groups: the first group (n=15), animals were treated with losartan (10 mgkg -1 body weight); the second group (n=15) was treated with lisinopril (10 mgkg -1 body weight); while the third group (n=10) was treated with proportional volume of 0.9% NaCl, daily for 12 weeks. For this purpose, histological analysis, RT-PCR of genes related to hepatic fibrosis, as well as TGF1 and p42/44 MAPK protein quantification were evaluated. There was observed a decrease in the collagen deposits in animals treated with losartan (p<0.01) and lisinopril (p<0.01) once compared to the controls. This improvement was accompanied by a reduction of -SMA labeled cells (p<0.001 and p<0.001) and procollagen 1(I) gene expression in both treated groups (p<0.05 and p<0.05). However, only losartan induced diminution of TGF1 and TIMP1 gene expression (p<0.01 and p<0.01), and TGF1 protein amount. Diversely, p42/44 MAPK protein was augmented in lisinopril group. Our findings demonstrated the effect of lisinopril and losartan on the decrease of hepatic fibrosis in murine schistosomiasis. The main mechanism of this process involves anti-fibrogenic activity via inhibition of TGF1 and TIMP1 by losartan treatment. Nevertheless, other possible mechanisms associated to lisinopril treatment may be implicated.
RESUMO: A deiscência de anastomose é uma das mais graves complicações advindas de operações do tu... more RESUMO: A deiscência de anastomose é uma das mais graves complicações advindas de operações do tubo gastrintestinal. Algumas condições gerais podem prejudicar o processo de cicatrização, tais como: desnutrição e hipoalbuminemia. Este estudo experimental teve como objetivo avaliar a cicatrização de anastomoses colônicas na vigência de desnutrição protéico-calórica e hipoalbuminemia. Dividimos os animais em dois grupos, sendo um deles o controle e o outro desnutrido (ingestão diária de metade da ração do grupo controle por vinte dias). O peso corporal, a albumina sérica, a evolução clínica, a cavidade abdominal, os aspectos macro e microscópicos da anastomose, a esteatose hepática e a concentração tecidual de hidroxiprolina foram observadas em cada animal. Pudemos notar que o método utilizado para desnutrir os animais mostrou-se eficaz, uma vez que houve redução significativa do peso do grupo experimental. Observamos que o grupo desnutrido apresentou dados necroscópicos de pior prognóstico e mortalidade superior ao grupo controle. Concluímos que a desnutrição influencia negativamente na cicatrização de anastomoses colônicas e aumenta significativamente a mortalidade. Descritores: desnutrição, anastomose colônica, cicatrização. FERREIRA MM; SCIALOM JM; CAMPOS AD; RAMALHO LLZ; MARCHINI JS; FÉRES O; ROCHA JJR. Efeito da Desnutrição na Cicatrização de Anastomoses Colônicas: Estudo Experimental em Ratos. Rev bras Coloproct, 2006;26(3): 239-243.
BioMed Research International, 2015
Nonalcoholic fatty liver disease (NAFLD) is characterized by hepatic steatosis, which occurs in t... more Nonalcoholic fatty liver disease (NAFLD) is characterized by hepatic steatosis, which occurs in the absence of alcohol abuse. NAFLD can evolve into progressive liver injury and fibrosis in the form of nonalcoholic steatohepatitis (NASH). Several animal models have been developed to attempt to represent the morphological, biochemical, and clinical features of human NASH. The actual review presents a critical analysis of the most commonly used experimental models of NAFLD/NASH development. These models can be classified into genetic, nutritional, and a combination of genetic and nutritional factors. The main genetic models are ob/ob and db/db mutant mice and Zucker rats. The principal nutritional models employ methionine-and choline-deficient, high-fat, high-cholesterol and high-cholate, cafeteria, and high-fructose diets. Currently, associations between high-fructose and various compositions of high-fat diets have been widely studied. Previous studies have encountered significant difficulties in developing animal models capable of reproducing human NASH. Some models produce consistent morphological findings, but the induction method differs significantly compared with the pathophysiology of human NASH. Other models precisely represent the clinical and etiological contexts of this disease but fail to provide accurate histopathological representations mainly in the progression from steatosis to liver fibrosis.
Archives of pathology & laboratory medicine, 2007
Although there is evidence that endometriosis results from basal endometrium dislocation, the und... more Although there is evidence that endometriosis results from basal endometrium dislocation, the underlying biology is not fully understood. One protein that plays an important role in regulating epithelial proliferation and differentiation is the 63-kDa membrane protein (p63), which is also a marker of basal and reserve cells in the female genital tract. To determine whether p63 is expressed differently in peritoneal endometriosis, endometriomas, and adenomyosis, as well as in deep endometriotic nodules of the rectovaginal septum and abdominal wall. This study includes a prospective series of consecutive patients (Canadian Task Force classification II-2) from a tertiary care university hospital. Specimens collected from 83 patients (15 peritoneal endometriosis specimens, 22 endometrioma specimens, 36 adenomyosis specimens, and 10 rectovaginal septum/abdominal wall specimens) were evaluated. Diagnostic and operative laparoscopies or laparotomies were performed, and tissue samples were ...
Journal of photochemistry and photobiology. B, Biology, 2015
Nitric oxide (NO) levels increase considerably after 24h of exposure of skin to ultraviolet B (UV... more Nitric oxide (NO) levels increase considerably after 24h of exposure of skin to ultraviolet B (UVB) radiation, which leads to nitrosative skin injury. In addition, increased NO levels after exposure to UVB radiation are associated with inhibition of cell proliferation. Compared to the UV-control group, UV-genistein at 10mg/kg (UV-GEN10) group showed tissue protection, decreased lipid peroxide and nitrotyrosine formation, and low CAT activity. Furthermore, NO levels and iNOS labeling remained high. In this group, the reduction in lipid peroxides and nitrotyrosine was accompanied by upregulation of cell proliferation factors (Ki67 and PCNA), which indicated that prevention of nitrosative skin injury promoted cell proliferation and DNA repair. Genistein also prevented nitrosative events, inhibited ONOO(-) formation, which leads to tissue protection and cell proliferation. The UV-GEN15 group did not result in a greater protective effect compared to that with UV-GEN10 group. In the UV-GE...
Acta Cirurgica Brasileira, 2001
ABSTRACT
Acta Cirurgica Brasileira, 2001
ABSTRACT
Acta Cirurgica Brasileira, 2001
ABSTRACT
Genetics and molecular research : GMR, 2005
Hereditary hemochromatosis (HH) is the most common genetic disease among individuals of European ... more Hereditary hemochromatosis (HH) is the most common genetic disease among individuals of European descent. Two mutations (845G-->A, C282Y and 187C-->G, H63D) in the hemochromatosis gene (HFE gene) are associated with HH. About 85-90% of patients of northern European descent with HH are C282Y homozygous. The prevalence of HH in the Brazilian population, which has a very high level of racial admixture, is unknown. The aims of the present study were to identify individuals with diagnostic criteria for HH among patients with a body iron overload attended at the university hospital of the Faculty of Medicine of Ribeirao Preto from 1990 to 2000, and to evaluate the prevalence of HFE mutations. We screened first-degree relatives for HFE mutations. Four of 72 patients (three men and one woman, mean age 47 years) fulfilled the criteria for HH. HFE mutations were studied in three patients [two C282Y homozygotes (patients 1 and 2) and one H63D heterozygote]. Patient 1 had four children (a...
Molecular Medicine Reports, 2008
This study aims to investigate human papilloma virus (HPV) frequency in Brazilian patients with o... more This study aims to investigate human papilloma virus (HPV) frequency in Brazilian patients with oral squamous cell carcinoma (OSCC) in order to establish a clinicopathological profile. It will also examine the correlation between patient survival and HPV expression in primary tumors (PTs), and their matched samples (MSs) of recidives, lymph nodal metastasis (LNM) or necropsies. Eighty-seven PTs and their corresponding 87 MSs were tested for HPV infection by polymerase chain reaction (PCR) using general and type-specific HPV primers. The following data were obtained from patient medical files: primary site, age, gender, tobacco consumption, histological differentiation, recurrences, metastasis, disease-free survival (DFS) and overall survival (OS). Of the 87 patients investigated, 17 (19.5%) were found to have HPV DNA in their tumors. An investigation of all the paraffin-embedded specimens revealed the presence of HPV DNA in 18 of the 174 samples (10.4%), 10 (11.5%) from PTs and 8 (9.2%) from MSs. No virus was detected in the corresponding PT of 7 (8.1%) MSs, and only one patient demonstrated HPV DNA positivity in both samples. The HPV genotypes 16 and 18 were detected in 4 (22.2%) and 3 (16.7%) of the positive samples, respectively. Infection with both genotypes was found in 6 (33.3%) investigated samples, and the HPV genotype was unidentified in 5 (27.8%) samples. The tongue was the most prevalent infected anatomical site. We did not find any significant association between HPV infection and gender, age, histological differentiation, DFS or OS. A significant number of HPV samples were positive among non-smoking patients. Although a possible influence of the virus on tumoral induction cannot be ruled out, the low frequency of OSCC cases that contain HPV does not suggest that this virus has the same etiological influence on patients as tobacco consumption does.
Statins exert beneficial effects in chronically damaged tissues. Angiotensin II (ANG II) particip... more Statins exert beneficial effects in chronically damaged tissues. Angiotensin II (ANG II) participates in liver fibrogenesis by inducing oxidative stress, inflammation, and transforming growth factor-beta1 (TGF-beta1) expression. We investigate whether atorvastatin modulates ANG II-induced pathogenic effects in the liver. Male Wistar rats were infused with saline or ANG II (100 ng kg(-1) min(-1)) for 4 wk through a subcutaneous osmotic pump. Rats received either vehicle or atorvastatin (5 mg kg(-1) day(-1)) by gavage. ANG II infusion resulted in infiltration of inflammatory cells (CD43 immunostaining), oxidative stress (4-hydroxynonenal), hepatic stellate cells (HSC) activation (smooth muscle alpha-actin), increased intercellular adhesion molecule (ICAM-1), and interleukin-6 hepatic gene expression (quantitative PCR). These effects were markedly blunted in rats receiving atorvastatin. The beneficial effects of atorvastatin were confirmed in an additional model of acute liver injury (carbon tetrachloride administration). We next explored whether the beneficial effects of atorvastatin on ANG II-induced actions are also reproduced at the cellular level. We studied HSC, a cell type with inflammatory and fibrogenic properties. ANG II (10(-8)M) stimulated cell proliferation, proinflammatory actions (NF-kappaB activation, ICAM-1 expression, interleukin-8 secretion) as well as expression of procollagen-alpha(1(I)) and TGF-beta1. All of these effects were reduced in the presence of atorvastatin (10(-7)M). These results indicate that atorvastatin attenuates the pathogenic events induced by ANG II in the liver both in vivo and in vitro. Therefore, statins could have beneficial effects in conditions characterized by hepatic inflammation.
ABSTRACT The inhibition of the renin-angiotensin system (RAS) display anti-fibrogenic effects in ... more ABSTRACT The inhibition of the renin-angiotensin system (RAS) display anti-fibrogenic effects in liver cirrhosis, however RAS influence on schistosomal fibrosis has not been totally clarified. Thus, our objective was to compare the activities of different antihypertensive drugs on the inhibition of the RAS in murine schistosomal fibrosis. BALB/c mice (n=40) weighing 20g were subjected to inoculation of 50 cercariae and subdivided in three groups: the first group (n=15), animals were treated with losartan (10 mgkg -1 body weight); the second group (n=15) was treated with lisinopril (10 mgkg -1 body weight); while the third group (n=10) was treated with proportional volume of 0.9% NaCl, daily for 12 weeks. For this purpose, histological analysis, RT-PCR of genes related to hepatic fibrosis, as well as TGF1 and p42/44 MAPK protein quantification were evaluated. There was observed a decrease in the collagen deposits in animals treated with losartan (p<0.01) and lisinopril (p<0.01) once compared to the controls. This improvement was accompanied by a reduction of -SMA labeled cells (p<0.001 and p<0.001) and procollagen 1(I) gene expression in both treated groups (p<0.05 and p<0.05). However, only losartan induced diminution of TGF1 and TIMP1 gene expression (p<0.01 and p<0.01), and TGF1 protein amount. Diversely, p42/44 MAPK protein was augmented in lisinopril group. Our findings demonstrated the effect of lisinopril and losartan on the decrease of hepatic fibrosis in murine schistosomiasis. The main mechanism of this process involves anti-fibrogenic activity via inhibition of TGF1 and TIMP1 by losartan treatment. Nevertheless, other possible mechanisms associated to lisinopril treatment may be implicated.