Leonardo Moreira - Academia.edu (original) (raw)
Papers by Leonardo Moreira
Jornal de Estudos Espíritas, 2020
Jornal de Estudos Espíritas, 2017
Jornal de Estudos Espíritas, 2016
Jornal de Estudos Espíritas, 2017
Jornal de Estudos Espíritas, 2015
Jornal de Estudos Espíritas, 2016
Resumo: O objetivo do presente estudo foi avaliar a suscetibilidade a antifúngicos de espécies de... more Resumo: O objetivo do presente estudo foi avaliar a suscetibilidade a antifúngicos de espécies de Trichosporon isoladas de diferentes fontes comparando duas metodologias: CLSI (Clinical and Laboratorial Standards Institute) e E-test. O perfil de suscetibilidade de 34 isolados foi testado contra anfotericina B, 5-flucitisina, fluconazol, itraconazol, voriconazol e terbinafina. A comparação entre as metodologias revelou maiores discrepâncias entre os resultados obtidos para o itraconazol e a 5-flucitosina. Palavras-chave: Trichosporon spp., antifúngicos, CLSI, E-test Área do Conhecimento: Saúde Introdução Trichosporon spp. podem causar infecções em seres humanos que variam de envolvimento cutâneo superficial a doenças sistêmicas em imunossuprimidos (WOLF et al., 2001). A tricosporonose é causada principalmente por seis espécies: T. asahiie T. mucoides são agents de infecções profundas, T. cutaneum e T. asteróides causam infecções superficiais e T. ovoides e T. inkin estão envolvidos n...
Brazilian journal of microbiology : [publication of the Brazilian Society for Microbiology], 2010
Several molecular methods, such as Southern blotting hybridization, Multilocus Sequence Typing, a... more Several molecular methods, such as Southern blotting hybridization, Multilocus Sequence Typing, and DNA microsatellite analysis, have been employed to genotype Candida albicans. The genotype analysis allows to group strains in clades, that is, a group composed of one ancestor and its descendants. These genotype studies demonstrate that clades distribution is influenced by geographic area as well as that antifungal resistance is associated with particular clades. These findings suggested that C. albicans reproduces mainly in a clonal manner, with certain degree of DNA microevolution. Additionally, virulence factors and site of isolation have also been associated with clade specificity. The present article is a brief review about the methods used for Candida genotyping and the correlated clade systems established. Special emphasis is given to Ca3 hybridization, MLST, and Microsatellites. The present work is also focused on the phenotypic and physiological traits associated with Candid...
Dyes and Pigments, 2015
Abstract In this work, the study on the influence of binary mixtures of solvents (water-acetonitr... more Abstract In this work, the study on the influence of binary mixtures of solvents (water-acetonitrile, water-ethanol and water-glycerol) upon the spectroscopic properties of methylene blue (MB) was done. In addition, the photophysical characterization of the MB in different concentrations in the solvent mixtures was done. In the mixtures, the increase in the quantity of water has decreased the fluorescence quantum yield together with other photophysical alterations. The studies of time-resolved fluorescence have demonstrated a first-order decay, with lifetimes between 328 and 550 ps. These values increase as the organic solvent proportion is increased. The results have shown a direct relationship between the viscosity and the rotational lifetime, correlating with the interference in the processes of deactivation of the excited state, which are slower in media with higher viscosity. The conformation of the clusters in the binary mixtures was also identified as a key factor to determine the results obtained in this work.
Spectroscopy, 2008
The present work focuses on the recent applications of Raman spectroscopy (RS) on biochemical ana... more The present work focuses on the recent applications of Raman spectroscopy (RS) on biochemical analysis and diagnosis of several biological materials with or without pathological alterations. Important published works about Raman spectroscopy and its use for medical applications were critically reviewed, including articles form our group in order to evaluate the state of the art of the subject. The potential for sample characterization with RS associated to the possibility of analysisin situmakes this instrumental technique in a very auspicious tool of biochemical analysis. RS can promote a significant improvement in the chemical identification and characterization of biological systems, clinical diagnosis and prognosis regarding several diseases and quality of life of innumerous patients. The spectroscopic evaluation is based on the analysis of the Raman spectrum regarding the identification of fingerprint bands of main biological macromolecules, such as nucleic acids, proteins and ...
Revista Brasileira de Saúde e Produção Animal, 2013
The decisive role of metallic cations in the formation of supramolecular clusters involving ligni... more The decisive role of metallic cations in the formation of supramolecular clusters involving lignin, cellulose, and hemi-cellulose and its relationship to energy losses in ruminants associated with fibrous feed resources is still not well understood. Indeed, interactions between lignin, cellulose and metallic cations generate highly stable clusters that significantly decrease the capability of cellulase to break bonds between sugar units in order to facilitate the absorption of a great quantity of cellulose, which is ingested by ruminants as forage. Furthermore, several metallic cations cannot be absorbed as a consequence of the formation of coordinated ligations with the oxygen atoms of the lignocellulosic cluster. The loss of lignocellulose-metal clusters by ruminants is responsible for a substantial waste of nutrients, which is a significant problem in animal science. Moreover, the chemical structure of these relevant supramolecular systems is poorly understood. In the present rev...
Revista Brasileira de Zootecnia, 2014
Spectroscopy, 2009
This work describes experimental tests done using the LED-therapy equipment developed for neonata... more This work describes experimental tests done using the LED-therapy equipment developed for neonatal jaundice treatment. Neonatal photpublisher-idapy consists in the irradiation of jaundice new-born with a high intensity light, in the spectral range of 400–500 nm, in order to promote the photochemical transformation of the bilirubin molecules in the areas exposed to the light. A convenient light source for jaundice treatment can be obtained employing blue LEDs, which are simple devices that present low cost, small heating production, narrow spectral band and long life-time. Equipment using an array with 88 blue LEDs (emission peak at 472 nm with 50 nm bandwidth) was constructed, optically characterized and then validated in comparison with a commercial equipment of photpublisher-idapy, i.e., the Fluorescent Conventional. For the device validation, experiments were undertaken employing male Wistar rats, to which the hyperbilirubinemia was induced by the obstruction of the billi duct. U...
Revista Brasileira de Saúde e Produção Animal, 2013
Forty-nine male calves were allotted in a randomized design, initially seven replicates per treat... more Forty-nine male calves were allotted in a randomized design, initially seven replicates per treatment at the end of the experiment were slaughtered four animals per treatment. Treatments CONT: control diet; FBC120: Dicalcium Phosphate 120:1 ratio P: F; MBC60: Monodicalcium phosphate 60:1 ratio P:F; FBC30: Dicalcium Phosphate 30:1 ratio P:F; SFT30: Super triple phosphate 30:1 ratio P:F; FBC10: Dicalcium Phosphate 10:1 ratio P:F; CAR10: concentrated ratio of 10:1 rock Q:F. Phosphates less elaborate showed higher amounts of heavy metals. In bones, iron, aluminum and lead were higher in FBC10 diet, the copper content was higher in animals fed the diet MBC60. Magnesium in liver was higher in animals fed diet FBC30, aluminum was higher for treatments SFT30 and FBC10, FBC120 and the cadmium and lead levels were higher in animals that received STF30. Treatments did not affect phosphorus and fluorine in muscle and heart, being that chromium was higher in the muscle of animals fed diets CAR10...
Spectroscopy, 2011
In this study, Raman spectroscopy was employed in order to provide information about the effects ... more In this study, Raman spectroscopy was employed in order to provide information about the effects of different doses of creatine on bone tissue composition of phosphate apatite (960 cm–1), carbonate apatite (1170 cm–1) representing the mineral content and collagen matrix (amide I, 1665 cm–1). The animals (27 Balb-C male) were divided into three groups (n═ per group): control (CON), supplemented with 0.5 g/kg (Cre-0.5) and with 2.0 g/kg (Cre-2.0) creatine. The experiment was carried out for thirty days. After this time, the right femur of each animal was harvested. The specimens were assessment by FT-Raman spectroscopy and in a total of 81 spectra were acquired in the medial diaphysis of the femur. The Raman data strongly suggest that only the creatine supplementation of 0.5 g/kg effective to the bone constitution. Furthermore, the present results demonstrate that creatine ingestion provokes decrease in the relative presence of carbonate in the chemical constitution of bones. The decr...
Effect of GaAs Laser at 904 nm in the Pain Threshold in Tibia and Tolerance in Deltoid Evaluated ... more Effect of GaAs Laser at 904 nm in the Pain Threshold in Tibia and Tolerance in Deltoid Evaluated by Pressure Algometry. [AIP Conference Proceedings 1364, 172 (2011)]. Luiz GP Soares, Sidney K. Sato, Landulfo Silveira, Jr ...
Journal of sports science & medicine, 2009
The purpose of the current study was to compare the workout volume (sets x resistance x repetitio... more The purpose of the current study was to compare the workout volume (sets x resistance x repetitions per set) completed during two upper body resistance exercise sessions that incorporated 1 minute versus 3 minute rest intervals between sets and exercises. Twelve trained men completed two experimental sessions that consisted of 5 upper body exercises (i.e. barbell bench press, incline barbell bench press, pec deck flye, barbell lying triceps extension, triceps pushdown) performed for three sets with an 8-RM load. The two experimental sessions differed only in the length of the rest interval between sets and exercises; one session with a 1-minute and the other session with a 3-minute rest interval. The results demonstrated that for each exercise, significantly greater workout volume was completed when resting 3 minutes between sets and exercises (p < 0.05). These results indicate that during a resistance exercise session, if sufficient time is available, resting 3 minutes between s...
Jornal de Estudos Espíritas, 2020
Jornal de Estudos Espíritas, 2017
Jornal de Estudos Espíritas, 2016
Jornal de Estudos Espíritas, 2017
Jornal de Estudos Espíritas, 2015
Jornal de Estudos Espíritas, 2016
Resumo: O objetivo do presente estudo foi avaliar a suscetibilidade a antifúngicos de espécies de... more Resumo: O objetivo do presente estudo foi avaliar a suscetibilidade a antifúngicos de espécies de Trichosporon isoladas de diferentes fontes comparando duas metodologias: CLSI (Clinical and Laboratorial Standards Institute) e E-test. O perfil de suscetibilidade de 34 isolados foi testado contra anfotericina B, 5-flucitisina, fluconazol, itraconazol, voriconazol e terbinafina. A comparação entre as metodologias revelou maiores discrepâncias entre os resultados obtidos para o itraconazol e a 5-flucitosina. Palavras-chave: Trichosporon spp., antifúngicos, CLSI, E-test Área do Conhecimento: Saúde Introdução Trichosporon spp. podem causar infecções em seres humanos que variam de envolvimento cutâneo superficial a doenças sistêmicas em imunossuprimidos (WOLF et al., 2001). A tricosporonose é causada principalmente por seis espécies: T. asahiie T. mucoides são agents de infecções profundas, T. cutaneum e T. asteróides causam infecções superficiais e T. ovoides e T. inkin estão envolvidos n...
Brazilian journal of microbiology : [publication of the Brazilian Society for Microbiology], 2010
Several molecular methods, such as Southern blotting hybridization, Multilocus Sequence Typing, a... more Several molecular methods, such as Southern blotting hybridization, Multilocus Sequence Typing, and DNA microsatellite analysis, have been employed to genotype Candida albicans. The genotype analysis allows to group strains in clades, that is, a group composed of one ancestor and its descendants. These genotype studies demonstrate that clades distribution is influenced by geographic area as well as that antifungal resistance is associated with particular clades. These findings suggested that C. albicans reproduces mainly in a clonal manner, with certain degree of DNA microevolution. Additionally, virulence factors and site of isolation have also been associated with clade specificity. The present article is a brief review about the methods used for Candida genotyping and the correlated clade systems established. Special emphasis is given to Ca3 hybridization, MLST, and Microsatellites. The present work is also focused on the phenotypic and physiological traits associated with Candid...
Dyes and Pigments, 2015
Abstract In this work, the study on the influence of binary mixtures of solvents (water-acetonitr... more Abstract In this work, the study on the influence of binary mixtures of solvents (water-acetonitrile, water-ethanol and water-glycerol) upon the spectroscopic properties of methylene blue (MB) was done. In addition, the photophysical characterization of the MB in different concentrations in the solvent mixtures was done. In the mixtures, the increase in the quantity of water has decreased the fluorescence quantum yield together with other photophysical alterations. The studies of time-resolved fluorescence have demonstrated a first-order decay, with lifetimes between 328 and 550 ps. These values increase as the organic solvent proportion is increased. The results have shown a direct relationship between the viscosity and the rotational lifetime, correlating with the interference in the processes of deactivation of the excited state, which are slower in media with higher viscosity. The conformation of the clusters in the binary mixtures was also identified as a key factor to determine the results obtained in this work.
Spectroscopy, 2008
The present work focuses on the recent applications of Raman spectroscopy (RS) on biochemical ana... more The present work focuses on the recent applications of Raman spectroscopy (RS) on biochemical analysis and diagnosis of several biological materials with or without pathological alterations. Important published works about Raman spectroscopy and its use for medical applications were critically reviewed, including articles form our group in order to evaluate the state of the art of the subject. The potential for sample characterization with RS associated to the possibility of analysisin situmakes this instrumental technique in a very auspicious tool of biochemical analysis. RS can promote a significant improvement in the chemical identification and characterization of biological systems, clinical diagnosis and prognosis regarding several diseases and quality of life of innumerous patients. The spectroscopic evaluation is based on the analysis of the Raman spectrum regarding the identification of fingerprint bands of main biological macromolecules, such as nucleic acids, proteins and ...
Revista Brasileira de Saúde e Produção Animal, 2013
The decisive role of metallic cations in the formation of supramolecular clusters involving ligni... more The decisive role of metallic cations in the formation of supramolecular clusters involving lignin, cellulose, and hemi-cellulose and its relationship to energy losses in ruminants associated with fibrous feed resources is still not well understood. Indeed, interactions between lignin, cellulose and metallic cations generate highly stable clusters that significantly decrease the capability of cellulase to break bonds between sugar units in order to facilitate the absorption of a great quantity of cellulose, which is ingested by ruminants as forage. Furthermore, several metallic cations cannot be absorbed as a consequence of the formation of coordinated ligations with the oxygen atoms of the lignocellulosic cluster. The loss of lignocellulose-metal clusters by ruminants is responsible for a substantial waste of nutrients, which is a significant problem in animal science. Moreover, the chemical structure of these relevant supramolecular systems is poorly understood. In the present rev...
Revista Brasileira de Zootecnia, 2014
Spectroscopy, 2009
This work describes experimental tests done using the LED-therapy equipment developed for neonata... more This work describes experimental tests done using the LED-therapy equipment developed for neonatal jaundice treatment. Neonatal photpublisher-idapy consists in the irradiation of jaundice new-born with a high intensity light, in the spectral range of 400–500 nm, in order to promote the photochemical transformation of the bilirubin molecules in the areas exposed to the light. A convenient light source for jaundice treatment can be obtained employing blue LEDs, which are simple devices that present low cost, small heating production, narrow spectral band and long life-time. Equipment using an array with 88 blue LEDs (emission peak at 472 nm with 50 nm bandwidth) was constructed, optically characterized and then validated in comparison with a commercial equipment of photpublisher-idapy, i.e., the Fluorescent Conventional. For the device validation, experiments were undertaken employing male Wistar rats, to which the hyperbilirubinemia was induced by the obstruction of the billi duct. U...
Revista Brasileira de Saúde e Produção Animal, 2013
Forty-nine male calves were allotted in a randomized design, initially seven replicates per treat... more Forty-nine male calves were allotted in a randomized design, initially seven replicates per treatment at the end of the experiment were slaughtered four animals per treatment. Treatments CONT: control diet; FBC120: Dicalcium Phosphate 120:1 ratio P: F; MBC60: Monodicalcium phosphate 60:1 ratio P:F; FBC30: Dicalcium Phosphate 30:1 ratio P:F; SFT30: Super triple phosphate 30:1 ratio P:F; FBC10: Dicalcium Phosphate 10:1 ratio P:F; CAR10: concentrated ratio of 10:1 rock Q:F. Phosphates less elaborate showed higher amounts of heavy metals. In bones, iron, aluminum and lead were higher in FBC10 diet, the copper content was higher in animals fed the diet MBC60. Magnesium in liver was higher in animals fed diet FBC30, aluminum was higher for treatments SFT30 and FBC10, FBC120 and the cadmium and lead levels were higher in animals that received STF30. Treatments did not affect phosphorus and fluorine in muscle and heart, being that chromium was higher in the muscle of animals fed diets CAR10...
Spectroscopy, 2011
In this study, Raman spectroscopy was employed in order to provide information about the effects ... more In this study, Raman spectroscopy was employed in order to provide information about the effects of different doses of creatine on bone tissue composition of phosphate apatite (960 cm–1), carbonate apatite (1170 cm–1) representing the mineral content and collagen matrix (amide I, 1665 cm–1). The animals (27 Balb-C male) were divided into three groups (n═ per group): control (CON), supplemented with 0.5 g/kg (Cre-0.5) and with 2.0 g/kg (Cre-2.0) creatine. The experiment was carried out for thirty days. After this time, the right femur of each animal was harvested. The specimens were assessment by FT-Raman spectroscopy and in a total of 81 spectra were acquired in the medial diaphysis of the femur. The Raman data strongly suggest that only the creatine supplementation of 0.5 g/kg effective to the bone constitution. Furthermore, the present results demonstrate that creatine ingestion provokes decrease in the relative presence of carbonate in the chemical constitution of bones. The decr...
Effect of GaAs Laser at 904 nm in the Pain Threshold in Tibia and Tolerance in Deltoid Evaluated ... more Effect of GaAs Laser at 904 nm in the Pain Threshold in Tibia and Tolerance in Deltoid Evaluated by Pressure Algometry. [AIP Conference Proceedings 1364, 172 (2011)]. Luiz GP Soares, Sidney K. Sato, Landulfo Silveira, Jr ...
Journal of sports science & medicine, 2009
The purpose of the current study was to compare the workout volume (sets x resistance x repetitio... more The purpose of the current study was to compare the workout volume (sets x resistance x repetitions per set) completed during two upper body resistance exercise sessions that incorporated 1 minute versus 3 minute rest intervals between sets and exercises. Twelve trained men completed two experimental sessions that consisted of 5 upper body exercises (i.e. barbell bench press, incline barbell bench press, pec deck flye, barbell lying triceps extension, triceps pushdown) performed for three sets with an 8-RM load. The two experimental sessions differed only in the length of the rest interval between sets and exercises; one session with a 1-minute and the other session with a 3-minute rest interval. The results demonstrated that for each exercise, significantly greater workout volume was completed when resting 3 minutes between sets and exercises (p < 0.05). These results indicate that during a resistance exercise session, if sufficient time is available, resting 3 minutes between s...