Leonardo Puerta - Academia.edu (original) (raw)

Papers by Leonardo Puerta

CRC Press eBooks, Feb 19, 2014

Revista de la Academia Colombiana de Ciencias Exactas, Físicas y Naturales, 2001

IP 1107-12-050-99ARTICULO(S) EN REVISTA: Obtencion y caracterizacion de unnovelalergeno mediante ... more IP 1107-12-050-99ARTICULO(S) EN REVISTA: Obtencion y caracterizacion de unnovelalergeno mediante la tecnologia del DNA;recombinante / Leonardo Puerta. -- En: Revista de la AcademiaColombiana de Ciencias Exactas, Fisica y;Natural. -- vol. 25, no. 94. ( mar. 2001); p. 77-87. -- ISSN 03703908. --Caracterizacion inmunoquimica y;molecular de los alergenos de Blomia tropicalis, un acarocausante de asmaen el tropico. -- En: Revista de la;Academia Colombiana de Ciencias Exactas, Fisica y Natural.--Vol. 23, no.88. (sep. 1999); p. 434-443. --;ISSN 03703908

Avances en Periodoncia e Implantología Oral, Dec 1, 2011

International Archives of Allergy and Immunology, 2023

Background: Exposure to mosquitoes in the Tropics is perennial, and their somatic and saliva anti... more Background: Exposure to mosquitoes in the Tropics is perennial, and their somatic and saliva antigens have shown IgE binding capacity, although it is not clear whether this is due to cross-reactivity or primary sensitization. Inhalation of these allergens could trigger an allergic response. Objective: The aim of the study was to evaluate the clinical relevance of sensitization to Aedes aegypti in a group of patients with allergic rhinitis. Methods: A cross-sectional study with allergic rhinitis subjects and healthy controls sensitized to mosquito extract was performed. Sensitization to mosquito and house dust mites was evaluated using skin prick test (SPT) and antibody determination by ELISA. Nasal provocation test (NPT) with whole-body extract was used to determine clinical relevance. Results: Allergic rhinitis patients were more sensitized to mosquito extract than controls with (+) SPT (66.6% vs. 7.6%). From these (+) SPT patients, 44.5% had (+) NPT, and just two (11%) presented mono-sensitization to mosquito. Antibody reactivity was similar between patients and controls; however, (+) NPT patients showed a tendency to had higher levels of IgE and IgG4. Discussion: Mosquitoes are perennial in most tropical areas, and their body allergens could be associated with respiratory allergies.

Revista Med, Jul 1, 2020

Resumen: Introducción: la infección por el virus del dengue es un problema de salud pública mundi... more Resumen: Introducción: la infección por el virus del dengue es un problema de salud pública mundial. El virus es transmitido por la picadura de mosquitos del género Aedes. Las proteínas de la saliva del vector Aedes aegypti inducen anticuerpos IgE e IgG4 específicos, cuya relación con la gravedad del dengue aún es desconocida. Objetivo: evaluar la asociación entre anticuerpos IgE e IgG4 específicos anti A. aegypti con la gravedad de la infección por dengue. Método: se realizó un estudio transversal en el que se incluyeron 16 niños con dengue grave (dg), 15 niños con dengue con signos de alarma (dcsa) y 26 niños sanos, todos menores de 15 años. Se determinaron niveles séricos de IgE e IgG4 específicas de A. aegypti; también se cuantificó vegf, sst2 y vegfr1 por elisa. Para las variables cualitativas se calcularon proporciones y odds ratio (or); en las variables cuantitativas se hallaron medianas, rango intercuartílico y se utilizó la prueba U Mann Whitney. Resultados: la oportunidad de los niños de tener dg con niveles séricos de IgG4 específica mayores de 0,5 od es 78 % menor [or=0,22] (ic de 95 % de 0,06-0,77), comparado con la oportunidad de tener dg con niveles séricos de IgG4 específica menores de 0,5 od. Plaquetas (p=0,0002) y vefg (p=0,003) más elevado en los pacientes con dcsa y sst2 fue más alto en el dg (p=0,004). Conclusión: niveles de anticuerpos de IgG4 anti A. aegypti se relacionan con menor gravedad clínica del dengue.

CRC Press eBooks, Feb 26, 2020

Journal of Investigational Allergology and Clinical Immunology, Feb 21, 2023

Supplementary Table 1. Description of serum features included in the cross-inhibition assays.

Background: The mite Blomia tropicalis (Blo t) is an important source of allergens and a major ri... more Background: The mite Blomia tropicalis (Blo t) is an important source of allergens and a major risk factor for allergic rhinitis and asthma. A hybrid protein that includes epitopes of allergens Blo t 1, Blo t 5, Blo t 10, Blo t 12 and Blo t 13 (BTx5) and shows low IgE reactivity in sera from house dust mite allergic subjects was reported previously. This study evaluated the BTx5 immune response in BALB/c mice. Methods: Mice were immunized intraperitoneally with BTx5 mixed with aluminum hydroxide and intranasal challenge with BTx5. Additionally, a group of mice was similarly treated with Blomia tropicalis and another group with PBS. Serum levels of specifi c IgE, IgG1, IgG2a and cytokines in bronchoalveolar lavage fl uid (BALF) were determined by ELISA. Airway infl ammatory response was evaluated by methacholine challenge and the cellular infi ltrate in lung tissue. The blocking eff ect of the IgG raised in mice immunized with BTx5 on IgE binding to Blo t extract was analyzed by ELIS...

International Archives of Allergy and Immunology, 2021

Introduction: Cross-reactivity between shrimp and house dust mite (HDM) proteins has been widely ... more Introduction: Cross-reactivity between shrimp and house dust mite (HDM) proteins has been widely documented. In tropical region, shrimp (5–15%) and mite sensitization (80–95%) is prevalent in allergic patients. However, the clinical relevance of shrimp sensitization in patients with allergic rhinitis (AR) has been poorly studied. The aim of this study was to determine the prevalence and the clinical relevance shrimp IgE sensitization in AR patients sensitized to Dermatophagoides pteronyssinus. Methods: The study was conducted in Medellin (Colombia). A cross-sectional study in patients with AR sensitized to HDM was performed in 3 steps: (i) assessment of IgE sensitization frequency to shrimp Penaeus azteca, Litopenaeus vannamei, and tropomyosin homologous allergens rDer p 10, rPen a 1, and rLit v 1, (ii) evaluation of the clinical relevance of shrimp sensitization using oral challenge test (OCT) and (iii) identification of possible risk factors for positive-OCT results. Ethical commi...

Allergy, 2017

Background: Saliva and muscle derived mosquito allergens have been purified and characterized. Ho... more Background: Saliva and muscle derived mosquito allergens have been purified and characterized. However, the complete set of allergens remains to be elucidated. In this study, we identified and characterized IgE-binding proteins from the mosquito species A. aegypti. Methods: Serum was obtained from 15 allergic individuals with asthma and/or rhinitis and sensitized to mosquito. IgE-binding was determined by ELISA. Total proteins from freeze dried bodies of A. aegypti were extracted and IgE reactive proteins were identified by 2D-gel electrophoresis, followed by Western blot with pooled or individual sera. IgE-reactive spots were further characterized by mass spectrometry. Results: Twenty five IgE-reactive spots were identified, corresponding to 10 different proteins, some of which appeared as different variants or isoforms. Heat shock cognate 70 (HSC-70) and Tropomyosin showed IgE reactivity with 60% of the sera, Lysosomal aspartic protease and "AAEL006070-PA" (Uniprot: Q177P3) with 40% Accepted Article This article is protected by copyright. All rights reserved. and the other proteins with less than 33.3% of the sera. Different variants, or isoforms of Tropomyosin, Arginine or Creatine kinase, Glyceraldehyde-3-phosphate dehydrogenase (GPDH), Calcium-binding protein and Phosphoglycerate mutase were also identified. The mixture of three allergens (Aed a 6, Aed a 8 and Aed a 10) seems to identify more than 80% of A. aegypti sensitized individuals, indicating that these allergens should be considered when designing of improved mosquito allergy diagnostic tools. Conclusions: The newly identified allergens may play a role in the pathophysiology of mosquito allergy in the Tropics, and some of them might be important arthropodrelated proteins involved in cross-reactivity between A. aegypti and other allergenic arthropods.

Journal of Allergy and Clinical Immunology, 2012

RATIONALE: To examine the relationship between asthma-related emergency department visits (AREDV)... more RATIONALE: To examine the relationship between asthma-related emergency department visits (AREDV) and the effect of tree pollen concentrations in a high asthma prevalence area, the New York City borough of the Bronx. METHODS: A retrospective search of daily adult and pediatric AREDVat two Bronx hospitals (Montefiore-Moses and Weiler) was conducted using the Clinical Looking Glass data analysis software. Daily counts for tree pollen from 3/2001 to 10/2008 were obtained from the Armonk counting station located in close proximity to the Bronx. All data were statistically analyzed and graphed as daily values. A regression analysis was used to determine the excess AREDV over the expected AREDV. Daily excess AREDV was compared to daily tree pollen concentrations. RESULTS: From 2001-2008, there were a total of 42,065 AREDV at the two hospitals. There were three distinct peaks of increased visits-in January, May, and November. For most of the years studied, the spring peak was most prominent and consistently overlapped with high tree pollen levels. In addition, our regression analysis revealed that the largest excess AREDV consistently overlapped the larger tree pollen levels during the month of May every year. CONCLUSIONS: There is a large, spring increase in AREDV in the Bronx that closely correlates with high tree pollen counts. This may have prognostic value in anticipating increased asthma exacerbations. Early warning of tree pollen peaks based on ongoing surveillance could potentially guide clinical practice and prevent AREDV in the Bronx.

International Journal of Molecular Sciences

Only few allergens derived from house dust mite (HDM) species have been evaluated in terms of the... more Only few allergens derived from house dust mite (HDM) species have been evaluated in terms of their potential to induce allergic inflammation. In this study, we aimed to evaluate different aspects of the allergenicity and allergenic activity of Blo t 2, a Blomia tropicalis allergen. Blo t 2 was produced as a recombinant protein in Escherichia coli. Its allergenic activity was tested in humans by skin prick test and basophil activation assays, and in mice, by passive cutaneous anaphylaxis and a model of allergic airway inflammation. Sensitization rate to Blo t 2 (54.3%) was similar to that found to Blo t 21 (57.2%) and higher than to Der p 2 (37.5%). Most Blo t 2-sensitized patients showed a low intensity response (99.5%). Blo t 2 elicited CD203c upregulation and allergen induced skin inflammation. Additionally, immunized animals produced anti-Blo t 2 IgE antibodies and passive transfer of their serum to non-immunized animals induced skin inflammation after allergen exposure. Immuniz...

International Archives of Allergy and Immunology

Background: Patients with allergic rhinitis to house dust mites have an increased risk of shrimp ... more Background: Patients with allergic rhinitis to house dust mites have an increased risk of shrimp allergy. Der p 10 is a candidate biomarker to predict the risk of shrimp allergy among allergic rhinitis patients. Objectives: The aim of this study was to evaluate the diagnostic performance of anti-Der p 10 IgE as a predictor of shrimp allergy. Methods: A nested case-control study was carried out with eighty-six allergic rhinitis patients sensitized to mite (Dermatophagoides pteronyssinus) and shrimp (Litopenaeu vannamei). Cases and controls were defined by anti-Der p 10 IgE results. Oral challenge with shrimp was used as the gold standard for the evaluation of diagnostic performance. Results: All shrimp oral challenge test (OCT)-positive patients were positive for IgE against Der p 10. The level of anti-Der p 10 IgE >1.2 kUA/mL had the best diagnostic performance (sensitivity 100%, specificity 65%) Conclusion: Anti-Der p 10 IgE is useful for predicting shrimp allergy diagnosis and ...

Journal of Allergy and Clinical Immunology, 1991

Regression analysis of ISAAC Centre characteristics and atopic conditions. (DOCX 14Â kb)

Overview of ISAAC centres by age and country, for wheeze and rhinoconjunctivitis. (XLS 122Â kb)