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Papers by Leonardo Santos

Pathology, 1996

Chromoblastomycosis, a localized chronic cutaneous and subcutaneous infection of the skin caused ... more Chromoblastomycosis, a localized chronic cutaneous and subcutaneous infection of the skin caused by pigmented fungi, is most common in the world's tropical and subtropical zones. The condition rarely occurs in Australia. We present 6 cases of chromoblastomycosis seen at the Royal Darwin Hospital, Northern Territory, from 1989 to 1994 and affecting predominantly male Caucasians ranging from 38 to 71 yrs of age. Clinically the lesions were verrucous or nodular. They mimicked basal or squamous cell carcinoma, nevi or solar keratoses. Histopathologic findings were nonspecific. The only pathognomonic finding was the presence of brown spores or sclerotic bodies within granulomata or within microabscesses in the skin.

Anais da Academia Brasileira de Ciências, 2015

Plants have the ability to undergo morphophysiological changes based on availability of light. Th... more Plants have the ability to undergo morphophysiological changes based on availability of light. The present study evaluated biomass accumulation, leaf morphoanatomy and physiology of Neonotonia wightii and Pueraria phaseoloides grown in full sunlight, as well as in 30% and 50% shade. Two assays were performed, one for each species, using a randomized block design with 10 replicates. A higher accumulation of fresh mass in the shoot of the plants was observed for both species under cultivation in 50% shade, while no differences were detected between the full sunlight and 30% shade. N. wightii and P. phaseoloides showed increase in area and reduction in thickness leaf when cultivated in 50% shade. There were no changes in photosynthetic rate, stomatal conductance, water use efficiency and evapotranspiration of P. phaseoloides plants because growth environment. However, the shade treatments caused alterations in physiological parameters of N. wightii. In both species, structural changes in the mesophyll occurred depending on the availability of light; however, the amount of leaf blade tissue remained unaltered. Despite the influence of light intensity variation on the morphophysiological plasticity of N. wightii and P. phaseoloides, no effects on biomass accumulation were observed in response to light.

PLoS ONE, 2009

Background: Cell therapy approaches for biologic cardiac repair hold great promises, although bas... more Background: Cell therapy approaches for biologic cardiac repair hold great promises, although basic fundamental issues remain poorly understood. In the present study we examined the effects of timing and routes of administration of bone marrow cells (BMC) post-myocardial infarction (MI) and the efficacy of an injectable biopolymer scaffold to improve cardiac cell retention and function.

Journal of Cardiac Failure, 2009

BackgroundThe purpose of the current study was to create a model of myocardial infarction (MI) th... more BackgroundThe purpose of the current study was to create a model of myocardial infarction (MI) that is homogeneous in size with a low immediate (24 hours) mortality.

Journal of Aging Research, 2010

Twenty five subjects were randomized to untrained (UN) and resistive-trained (RT) groups. The RT ... more Twenty five subjects were randomized to untrained (UN) and resistive-trained (RT) groups. The RT group exercised three sessions per week at 60%-70% of the load according to individual 1RM test during 24 weeks. Both groups were evaluated before and after protocol period assessing lumbar spine (LS) and femoral neck (FN) BMD by dual-energy X-ray absorptiometry, VO 2 max, and neuromuscular fitness. After 24 weeks, there were significant reductions in LS (0.89 ± 0.16% loss) and FN BMD (1.54 ± 0.35% loss) for UN but no change was found in the TR (LS: 0.01 ± 0.12% and FN: 0.04 ± 0.05% loss). The UN group had no changes in neuromuscular performance. However, RT exhibited a significant improvement on the functional fitness parameters evaluated, with the exception of agility. Our results indicate RT suppresses the decline in BMD and simultaneously improves the functional fitness of postmenopausal women without hormone replacement therapy, which may reduce fall risk and related bone fractures. of Hindawi Publishing Corporation

PLoS ONE, 2010

Background: Cardiac cell transplantation is compromised by low cell retention and poor graft viab... more Background: Cardiac cell transplantation is compromised by low cell retention and poor graft viability. Here, the effects of co-injecting adipose tissue-derived stem cells (ASCs) with biopolymers on cell cardiac retention, ventricular morphometry and performance were evaluated in a rat model of myocardial infarction (MI).

Pathology, 2001

Close Window. Close Window. Thank you for choosing to subscribe to the eTOC for Pathology. Enter ... more Close Window. Close Window. Thank you for choosing to subscribe to the eTOC for Pathology. Enter your Email address: Wolters Kluwer Health may email you for journal alerts and information, but is committed to maintaining ...

Pathology, 2002

We reviewed all cases of pilonidal sinus excision specimens from our surgical pathology records f... more We reviewed all cases of pilonidal sinus excision specimens from our surgical pathology records from 1990 to 2000 to determine the presence of glomus coccygeum. We found only two cases of glomus coccygeum, also known as coccygeal body. We describe the histology and immunohistochemical findings of two glomera coccygea incidentally discovered in pilonidal sinus excision specimens, and review the world's English literature.

Pathology, 2002

Cystic neoplasms of the pancreas are uncommon lesions but are becoming increasingly prevalent. He... more Cystic neoplasms of the pancreas are uncommon lesions but are becoming increasingly prevalent. Herein we report a case of an oligolocular cystic lesion in the head of the pancreas in a young female that had undergone a cystenteroanastomosis 10 years ago. She underwent a duodenopancreatectomy with an uneventful recovery. The histology showed a serous oligocystic adenoma of the pancreas and the immunohistochemistry study confirmed the diagnosis. There is no sign of recurrence after the surgery. The role of pre-operative diagnosis based on tomographic, echoendoscopy and fine needle aspiration are discussed.

Pathology, 2006

There has been very little mention of benign solid lesions of the Bartholin&a... more There has been very little mention of benign solid lesions of the Bartholin's gland (BG) in pathology and gynaecology textbooks, and very few cases have been reported in the literature. Among these lesions, the distinction between nodular hyperplasia (NH) and adenoma has not been well defined. We report ten cases of NH of the BG, describe their clinicopathological, immunohistochemical and ultrastructural findings, and review the literature. We examined retrospectively all lesions involving BGs from our surgical pathology records from 1990 to 2004 with emphasis on NH. To separate NH from adenoma, we applied the criteria proposed by Koenig and Tavassoli. Special stains (PAS with and without prior digestion, Mayer's mucicarmine and Alcian blue with and without hyaluronidase) and immunohistochemistry (CAM5.2, AE1/AE3, HMWK, monoclonal CEA, EMA, ER, PR, ALA, SMA, Ki-67, p53 and polyclonal CEA) were performed on NHs. Two cases were examined ultrastructurally. Using specific criteria, ten cases (age range 23-45 years; mean 36.1) of NH were identified, two of which were diagnosed previously as adenoma, but re-classified as NH. Clinically, these lesions were described either as Bartholin's duct cysts (BDCs) or vulvar lumps. Grossly, NHs were solid, tan and unencapsulated, measuring 12.5-45.0 mm in maximum dimension (mean 23.8). Histologically, the NHs were composed of a proliferation of mucus-secreting acini with preservation of the normal duct-to-acinar relationship. Chronic inflammation and squamous metaplasia were present. Eight lesions focally involved the surgical margins. Intracytoplasmic and intra-luminal secretions were positive for PAS with and without digestion, Alcian blue with and without hyaluronidase and mucicarmine. All lesions showed positive staining for CAM5.2, AE1/AE3, HMWK, EMA, and polyclonal CEA. There was negative staining for Ki-67, ER, PR, ALA, p53 and monoclonal CEA. Periacinar myoepithelial cells stained for SMA. Ultrastructurally, the findings included abundant intracytoplasmic secretory granules, granulofibrillar bodies, prominent Golgi and ribosomes. Myoepithelial cells were identified. There was no tumour recurrence or malignant transformation in eight patients with clinical follow-up. NH of the BG is a rare lesion with benign behaviour. It is a distinct entity and can be separated histologically from an adenoma.

Pathology, 1996

Chromoblastomycosis, a localized chronic cutaneous and subcutaneous infection of the skin caused ... more Chromoblastomycosis, a localized chronic cutaneous and subcutaneous infection of the skin caused by pigmented fungi, is most common in the world's tropical and subtropical zones. The condition rarely occurs in Australia. We present 6 cases of chromoblastomycosis seen at the Royal Darwin Hospital, Northern Territory, from 1989 to 1994 and affecting predominantly male Caucasians ranging from 38 to 71 yrs of age. Clinically the lesions were verrucous or nodular. They mimicked basal or squamous cell carcinoma, nevi or solar keratoses. Histopathologic findings were nonspecific. The only pathognomonic finding was the presence of brown spores or sclerotic bodies within granulomata or within microabscesses in the skin.

Anais da Academia Brasileira de Ciências, 2015

Plants have the ability to undergo morphophysiological changes based on availability of light. Th... more Plants have the ability to undergo morphophysiological changes based on availability of light. The present study evaluated biomass accumulation, leaf morphoanatomy and physiology of Neonotonia wightii and Pueraria phaseoloides grown in full sunlight, as well as in 30% and 50% shade. Two assays were performed, one for each species, using a randomized block design with 10 replicates. A higher accumulation of fresh mass in the shoot of the plants was observed for both species under cultivation in 50% shade, while no differences were detected between the full sunlight and 30% shade. N. wightii and P. phaseoloides showed increase in area and reduction in thickness leaf when cultivated in 50% shade. There were no changes in photosynthetic rate, stomatal conductance, water use efficiency and evapotranspiration of P. phaseoloides plants because growth environment. However, the shade treatments caused alterations in physiological parameters of N. wightii. In both species, structural changes in the mesophyll occurred depending on the availability of light; however, the amount of leaf blade tissue remained unaltered. Despite the influence of light intensity variation on the morphophysiological plasticity of N. wightii and P. phaseoloides, no effects on biomass accumulation were observed in response to light.

PLoS ONE, 2009

Background: Cell therapy approaches for biologic cardiac repair hold great promises, although bas... more Background: Cell therapy approaches for biologic cardiac repair hold great promises, although basic fundamental issues remain poorly understood. In the present study we examined the effects of timing and routes of administration of bone marrow cells (BMC) post-myocardial infarction (MI) and the efficacy of an injectable biopolymer scaffold to improve cardiac cell retention and function.

Journal of Cardiac Failure, 2009

BackgroundThe purpose of the current study was to create a model of myocardial infarction (MI) th... more BackgroundThe purpose of the current study was to create a model of myocardial infarction (MI) that is homogeneous in size with a low immediate (24 hours) mortality.

Journal of Aging Research, 2010

Twenty five subjects were randomized to untrained (UN) and resistive-trained (RT) groups. The RT ... more Twenty five subjects were randomized to untrained (UN) and resistive-trained (RT) groups. The RT group exercised three sessions per week at 60%-70% of the load according to individual 1RM test during 24 weeks. Both groups were evaluated before and after protocol period assessing lumbar spine (LS) and femoral neck (FN) BMD by dual-energy X-ray absorptiometry, VO 2 max, and neuromuscular fitness. After 24 weeks, there were significant reductions in LS (0.89 ± 0.16% loss) and FN BMD (1.54 ± 0.35% loss) for UN but no change was found in the TR (LS: 0.01 ± 0.12% and FN: 0.04 ± 0.05% loss). The UN group had no changes in neuromuscular performance. However, RT exhibited a significant improvement on the functional fitness parameters evaluated, with the exception of agility. Our results indicate RT suppresses the decline in BMD and simultaneously improves the functional fitness of postmenopausal women without hormone replacement therapy, which may reduce fall risk and related bone fractures. of Hindawi Publishing Corporation

PLoS ONE, 2010

Background: Cardiac cell transplantation is compromised by low cell retention and poor graft viab... more Background: Cardiac cell transplantation is compromised by low cell retention and poor graft viability. Here, the effects of co-injecting adipose tissue-derived stem cells (ASCs) with biopolymers on cell cardiac retention, ventricular morphometry and performance were evaluated in a rat model of myocardial infarction (MI).

Pathology, 2001

Close Window. Close Window. Thank you for choosing to subscribe to the eTOC for Pathology. Enter ... more Close Window. Close Window. Thank you for choosing to subscribe to the eTOC for Pathology. Enter your Email address: Wolters Kluwer Health may email you for journal alerts and information, but is committed to maintaining ...

Pathology, 2002

We reviewed all cases of pilonidal sinus excision specimens from our surgical pathology records f... more We reviewed all cases of pilonidal sinus excision specimens from our surgical pathology records from 1990 to 2000 to determine the presence of glomus coccygeum. We found only two cases of glomus coccygeum, also known as coccygeal body. We describe the histology and immunohistochemical findings of two glomera coccygea incidentally discovered in pilonidal sinus excision specimens, and review the world's English literature.

Pathology, 2002

Cystic neoplasms of the pancreas are uncommon lesions but are becoming increasingly prevalent. He... more Cystic neoplasms of the pancreas are uncommon lesions but are becoming increasingly prevalent. Herein we report a case of an oligolocular cystic lesion in the head of the pancreas in a young female that had undergone a cystenteroanastomosis 10 years ago. She underwent a duodenopancreatectomy with an uneventful recovery. The histology showed a serous oligocystic adenoma of the pancreas and the immunohistochemistry study confirmed the diagnosis. There is no sign of recurrence after the surgery. The role of pre-operative diagnosis based on tomographic, echoendoscopy and fine needle aspiration are discussed.

Pathology, 2006

There has been very little mention of benign solid lesions of the Bartholin&a... more There has been very little mention of benign solid lesions of the Bartholin's gland (BG) in pathology and gynaecology textbooks, and very few cases have been reported in the literature. Among these lesions, the distinction between nodular hyperplasia (NH) and adenoma has not been well defined. We report ten cases of NH of the BG, describe their clinicopathological, immunohistochemical and ultrastructural findings, and review the literature. We examined retrospectively all lesions involving BGs from our surgical pathology records from 1990 to 2004 with emphasis on NH. To separate NH from adenoma, we applied the criteria proposed by Koenig and Tavassoli. Special stains (PAS with and without prior digestion, Mayer's mucicarmine and Alcian blue with and without hyaluronidase) and immunohistochemistry (CAM5.2, AE1/AE3, HMWK, monoclonal CEA, EMA, ER, PR, ALA, SMA, Ki-67, p53 and polyclonal CEA) were performed on NHs. Two cases were examined ultrastructurally. Using specific criteria, ten cases (age range 23-45 years; mean 36.1) of NH were identified, two of which were diagnosed previously as adenoma, but re-classified as NH. Clinically, these lesions were described either as Bartholin's duct cysts (BDCs) or vulvar lumps. Grossly, NHs were solid, tan and unencapsulated, measuring 12.5-45.0 mm in maximum dimension (mean 23.8). Histologically, the NHs were composed of a proliferation of mucus-secreting acini with preservation of the normal duct-to-acinar relationship. Chronic inflammation and squamous metaplasia were present. Eight lesions focally involved the surgical margins. Intracytoplasmic and intra-luminal secretions were positive for PAS with and without digestion, Alcian blue with and without hyaluronidase and mucicarmine. All lesions showed positive staining for CAM5.2, AE1/AE3, HMWK, EMA, and polyclonal CEA. There was negative staining for Ki-67, ER, PR, ALA, p53 and monoclonal CEA. Periacinar myoepithelial cells stained for SMA. Ultrastructurally, the findings included abundant intracytoplasmic secretory granules, granulofibrillar bodies, prominent Golgi and ribosomes. Myoepithelial cells were identified. There was no tumour recurrence or malignant transformation in eight patients with clinical follow-up. NH of the BG is a rare lesion with benign behaviour. It is a distinct entity and can be separated histologically from an adenoma.