Levente Emődy - Academia.edu (original) (raw)
Papers by Levente Emődy
Acta microbiologica Academiae Scientiarum Hungaricae, 1972
ABSTRACT
Acta microbiologica Academiae Scientiarum Hungaricae, 1972
Acta microbiologica Academiae Scientiarum Hungaricae, 1972
ABSTRACT
Zentralblatt für Bakteriologie, Parasitenkunde, Infektionskrankheiten und Hygiene. Erste Abteilung Originale. Reihe A: Medizinische Mikrobiologie und Parasitologie, 1972
Journal of Bacteriology, Jul 1, 1992
Four strains of diarrheagenic Escherichia coli originally isolated from distinct geographic regio... more Four strains of diarrheagenic Escherichia coli originally isolated from distinct geographic regions were found to produce unusual thin aggregative fimbriae requiring depolymerization in formic acid prior to analysis by sodium dodecyl sulfate-polyacrylamide gel electrophoresis. Immunoelectron microscopy of native fimbriae and Western blot (immunoblot) analysis of the corresponding 18-kDa fimbrins showed that these E. coli fimbriae were serologically cross-reactive with SEF 17 (Salmonella enteritidis fimbriae with a fimbrin molecular mass of 17 kDa). The E. coli and S. enteritidis fimbrins had similar total amino acid compositions and highly conserved * Corresponding author.
Journal of Bacteriology, 1989
Binding of Yersinia enterocolitica and Yersinia pseudotuberculosis strains to type I, II, and IV ... more Binding of Yersinia enterocolitica and Yersinia pseudotuberculosis strains to type I, II, and IV collagens has been studied. Wild-type strains which harbored the 40- to 50-megadalton virulence plasmid specifically bound all three types of collagen. Curing of the virulence plasmid or Tn5 insertion in the yopA gene encoding the temperature-inducible outer membrane protein YOP1 abolished the binding of all three collagen types to Y. enterocolitica and type I and II collagens to Y. pseudotuberculosis. Full binding capacity was restored by introduction of the yopA gene into nonbinding Yersinia strains. Binding of type I, II, and IV collagens was expressed in Escherichia coli constructs harboring the yopA gene of either Y. enterocolitica or Y. pseudotuberculosis. The interaction of bacterial cells with type I collagen could be blocked by nonradiolabeled native collagens or denatured collagen but not with other serum and connective-tissue proteins. Unlabeled collagen could not displace bou...
Journal of Biological Chemistry, 1992
BioMed Research International, 2019
Campylobacter jejuniis one of the most common food-borne bacteria that causes gastrointestinal sy... more Campylobacter jejuniis one of the most common food-borne bacteria that causes gastrointestinal symptoms. In the present study we have investigated the molecular basis of the anti-Campylobactereffect of peppermint essential oil (PEO), one of the oldest EO used to treat gastrointestinal diseases. Transcriptomic, quantitative reverse transcription-polymerase chain reaction (qRT-PCR) and proteomic, two-dimensional polyacryl amid gel electrophoresis (2D-PAGE) methods have revealed that, in the presence of a sublethal concentration of PEO, the expression of several virulence-associated genes was decreased (cheY0.84x;flhB0.79x;flgE0.205x;cadF0.08x;wlaB0.89x;porA0.25x;cbf24.3x) while impaired motility was revealed with a functional analysis. Scanning electron micrographs of the exposed cells showed that, unlike in the presence of other stresses, the originally curvedC. jejunicells straightened upon PEO exposure. Gaining insight into the molecular background of this stress response, we have ...
Mobilisation and remobilisation of a large archetypal pathogenicity island of uropathogenic Esche... more Mobilisation and remobilisation of a large archetypal pathogenicity island of uropathogenic Escherichia coli in vitro support the role of conjugation for horizontal transfer of genomic islands
Infection and Immunity, 1991
The ability of 16 isolates of the human gastroduodenal pathogen Helicobacter pylori to bind 125I-... more The ability of 16 isolates of the human gastroduodenal pathogen Helicobacter pylori to bind 125I-radiolabelled tissue proteins was quantitated by liquid-phase assay. While capable of binding generally low levels of collagen types I and II, vitronectin, and fibronectin (average binding, 8%; highest binding, 23%), the various H. pylori isolates were good binders of the basement membrane proteins collagen type IV and laminin (average binding, 27%; highest binding, 60%). Campylobacter species tested bound lower levels of collagen type IV and laminin (average binding, 12%; highest binding, 17%). Trypsin and proteinase K treatment of H. pylori cells markedly reduced the binding of collagen type IV and laminin, as did heat treatment, suggesting that the binding of basement membrane proteins is mediated by bacterial surface proteins. Binding of both basement membrane proteins was rapid and saturable. 125I-collagen type IV binding to H. pylori 915 was inhibited by preincubation with unlabell...
Infection and Immunity, 1998
The effect of the plasminogen activator Pla of Yersinia pestis on the adhesiveness of bacteria to... more The effect of the plasminogen activator Pla of Yersinia pestis on the adhesiveness of bacteria to the mammalian extracellular matrix was determined. Y. pestis KIM D27 harbors the 9.5-kb plasmid pPCP1, encoding Pla and pesticin; the strain efficiently adhered to the reconstituted basement membrane preparation Matrigel, to the extracellular matrix prepared from human lung NCI-H292 epithelial cells, as well as to immobilized laminin. The isogenic strain Y. pestis KIM D34 lacking pPCP1 exhibited lower adhesiveness to both matrix preparations and to laminin. Both strains showed weak adherence to type I, IV, and V collagens as well as to human plasma and cellular fibronectin. The Pla-expressing recombinant Escherichia coli LE392(pC4006) exhibited specific adhesiveness to both extracellular matrix preparations as well as to laminin. The Pla-expressing strains showed a low-affinity adherence to another basement membrane component, heparan sulfate proteoglycan, but not to chondroitin sulfate...
Applied and Environmental Microbiology, 1997
Legionella pneumophila is an aquatic bacterium and is responsible for Legionnaires' disease i... more Legionella pneumophila is an aquatic bacterium and is responsible for Legionnaires' disease in humans. Free-living amoebae are parasitized by legionellae and provide the intracellular environment required for the replication of this bacterium. In low-nutrient environments, however, L. pneumophila is able to enter a non-replicative viable but nonculturable (VBNC) state. In this study, L. pneumophila Philadelphia I JR 32 was suspended in sterilized tap water at 10(4) cells/ml. The decreasing number of bacteria was monitored by CFU measurements, acridine orange direct count (AODC), and hybridization with 16S rRNA-targeted oligonucleotide probes. After 125 days of incubation in water, the cells were no longer culturable on routine plating media; however, they were still detectable by AODC and by in situ hybridization. The addition of Acanthamoeba castellanii to the dormant bacteria resulted in the resuscitation of L. pneumophila JR 32 to a culturable state. A comparison of plate-gro...
Frontiers in microbiology, 2018
Asymptomatic bacterial colonization of the urinary bladder (asymptomatic bacteriuria, ABU) can pr... more Asymptomatic bacterial colonization of the urinary bladder (asymptomatic bacteriuria, ABU) can prevent bladder colonization by uropathogens and thus symptomatic urinary tract infection (UTI). Deliberate bladder colonization withABU isolate 83972 has been shown to outcompete uropathogens and prevent symptomatic UTI by bacterial interference. Many ABU isolates evolved from uropathogenic ancestors and, although attenuated, may still be able to express virulence-associated factors. Our aim was to screen for efficient and safe candidate strains that could be used as alternatives to83972 for preventive and therapeutic bladder colonization. To identify ABUstrains with minimal virulence potential but maximal interference efficiency, we compared nine ABU isolates from diabetic patients regarding their virulence- and fitness-associated phenotypes, their virulence in a murine model of sepsis and their genome content. We identified strains in competitive growth experiments, which successfully i...
Journal of Bacteriology, 1991
Novel fimbriae were isolated and purified from the human enteropathogen Salmonella enteritidis 27... more Novel fimbriae were isolated and purified from the human enteropathogen Salmonella enteritidis 27655. These fimbriae were thin (measuring 3 to 4 nm in diameter), were extremely aggregative, and remained cell associated despite attempts to separate them from blended cells by centrifugation. The thin fimbriae were not solubilized in 5 M NaOH or in boiling 0.5% deoxycholate, 8 M urea, or 1 to 2% sodium dodecyl sulfate (SDS) with or without 5% beta-mercaptoethanol. Therefore, an unconventional purification procedure based on the removal of contaminating cell macromolecules in sonicated cell extracts by enzymatic digestion and preparative SDS-polyacrylamide gel electrophoresis (PAGE) was used. The insoluble fimbriae recovered from the well of the gel required depolymerization in formic acid prior to analysis by SDS-PAGE. Acid depolymerization revealed that the fimbriae were composed of fimbrin subunits, each with an apparent molecular mass of 17 kDa. Although their biochemical characteri...
Advances in Experimental Medicine and Biology, 2002
... 3. Blum, G., Ott, M., Lischewski, A., Ritter, A., Imrich, H., Tschape, H., and Hacker, J., 19... more ... 3. Blum, G., Ott, M., Lischewski, A., Ritter, A., Imrich, H., Tschape, H., and Hacker, J., 1994, Excision of large DNA region termed ... 6. Oswald, E., personal communication 7. Le Bouguenec, C., Archaumband, M., and Labigne, A., 1992, Rapid and specific detection ofpap, afa, and ...
Advances in Experimental Medicine and Biology
Escherichia coli is by far the most common causative agent of urinary tract infections. The abili... more Escherichia coli is by far the most common causative agent of urinary tract infections. The ability of uropathogenic strains to produce certain virulence factors distinguishes them from commensal and intestinal pathogenic strains. Pathogenicity factors, such as fimbriae, ferric uptake ...
Acta microbiologica Academiae Scientiarum Hungaricae, 1972
ABSTRACT
Acta microbiologica Academiae Scientiarum Hungaricae, 1972
Acta microbiologica Academiae Scientiarum Hungaricae, 1972
ABSTRACT
Zentralblatt für Bakteriologie, Parasitenkunde, Infektionskrankheiten und Hygiene. Erste Abteilung Originale. Reihe A: Medizinische Mikrobiologie und Parasitologie, 1972
Journal of Bacteriology, Jul 1, 1992
Four strains of diarrheagenic Escherichia coli originally isolated from distinct geographic regio... more Four strains of diarrheagenic Escherichia coli originally isolated from distinct geographic regions were found to produce unusual thin aggregative fimbriae requiring depolymerization in formic acid prior to analysis by sodium dodecyl sulfate-polyacrylamide gel electrophoresis. Immunoelectron microscopy of native fimbriae and Western blot (immunoblot) analysis of the corresponding 18-kDa fimbrins showed that these E. coli fimbriae were serologically cross-reactive with SEF 17 (Salmonella enteritidis fimbriae with a fimbrin molecular mass of 17 kDa). The E. coli and S. enteritidis fimbrins had similar total amino acid compositions and highly conserved * Corresponding author.
Journal of Bacteriology, 1989
Binding of Yersinia enterocolitica and Yersinia pseudotuberculosis strains to type I, II, and IV ... more Binding of Yersinia enterocolitica and Yersinia pseudotuberculosis strains to type I, II, and IV collagens has been studied. Wild-type strains which harbored the 40- to 50-megadalton virulence plasmid specifically bound all three types of collagen. Curing of the virulence plasmid or Tn5 insertion in the yopA gene encoding the temperature-inducible outer membrane protein YOP1 abolished the binding of all three collagen types to Y. enterocolitica and type I and II collagens to Y. pseudotuberculosis. Full binding capacity was restored by introduction of the yopA gene into nonbinding Yersinia strains. Binding of type I, II, and IV collagens was expressed in Escherichia coli constructs harboring the yopA gene of either Y. enterocolitica or Y. pseudotuberculosis. The interaction of bacterial cells with type I collagen could be blocked by nonradiolabeled native collagens or denatured collagen but not with other serum and connective-tissue proteins. Unlabeled collagen could not displace bou...
Journal of Biological Chemistry, 1992
BioMed Research International, 2019
Campylobacter jejuniis one of the most common food-borne bacteria that causes gastrointestinal sy... more Campylobacter jejuniis one of the most common food-borne bacteria that causes gastrointestinal symptoms. In the present study we have investigated the molecular basis of the anti-Campylobactereffect of peppermint essential oil (PEO), one of the oldest EO used to treat gastrointestinal diseases. Transcriptomic, quantitative reverse transcription-polymerase chain reaction (qRT-PCR) and proteomic, two-dimensional polyacryl amid gel electrophoresis (2D-PAGE) methods have revealed that, in the presence of a sublethal concentration of PEO, the expression of several virulence-associated genes was decreased (cheY0.84x;flhB0.79x;flgE0.205x;cadF0.08x;wlaB0.89x;porA0.25x;cbf24.3x) while impaired motility was revealed with a functional analysis. Scanning electron micrographs of the exposed cells showed that, unlike in the presence of other stresses, the originally curvedC. jejunicells straightened upon PEO exposure. Gaining insight into the molecular background of this stress response, we have ...
Mobilisation and remobilisation of a large archetypal pathogenicity island of uropathogenic Esche... more Mobilisation and remobilisation of a large archetypal pathogenicity island of uropathogenic Escherichia coli in vitro support the role of conjugation for horizontal transfer of genomic islands
Infection and Immunity, 1991
The ability of 16 isolates of the human gastroduodenal pathogen Helicobacter pylori to bind 125I-... more The ability of 16 isolates of the human gastroduodenal pathogen Helicobacter pylori to bind 125I-radiolabelled tissue proteins was quantitated by liquid-phase assay. While capable of binding generally low levels of collagen types I and II, vitronectin, and fibronectin (average binding, 8%; highest binding, 23%), the various H. pylori isolates were good binders of the basement membrane proteins collagen type IV and laminin (average binding, 27%; highest binding, 60%). Campylobacter species tested bound lower levels of collagen type IV and laminin (average binding, 12%; highest binding, 17%). Trypsin and proteinase K treatment of H. pylori cells markedly reduced the binding of collagen type IV and laminin, as did heat treatment, suggesting that the binding of basement membrane proteins is mediated by bacterial surface proteins. Binding of both basement membrane proteins was rapid and saturable. 125I-collagen type IV binding to H. pylori 915 was inhibited by preincubation with unlabell...
Infection and Immunity, 1998
The effect of the plasminogen activator Pla of Yersinia pestis on the adhesiveness of bacteria to... more The effect of the plasminogen activator Pla of Yersinia pestis on the adhesiveness of bacteria to the mammalian extracellular matrix was determined. Y. pestis KIM D27 harbors the 9.5-kb plasmid pPCP1, encoding Pla and pesticin; the strain efficiently adhered to the reconstituted basement membrane preparation Matrigel, to the extracellular matrix prepared from human lung NCI-H292 epithelial cells, as well as to immobilized laminin. The isogenic strain Y. pestis KIM D34 lacking pPCP1 exhibited lower adhesiveness to both matrix preparations and to laminin. Both strains showed weak adherence to type I, IV, and V collagens as well as to human plasma and cellular fibronectin. The Pla-expressing recombinant Escherichia coli LE392(pC4006) exhibited specific adhesiveness to both extracellular matrix preparations as well as to laminin. The Pla-expressing strains showed a low-affinity adherence to another basement membrane component, heparan sulfate proteoglycan, but not to chondroitin sulfate...
Applied and Environmental Microbiology, 1997
Legionella pneumophila is an aquatic bacterium and is responsible for Legionnaires' disease i... more Legionella pneumophila is an aquatic bacterium and is responsible for Legionnaires' disease in humans. Free-living amoebae are parasitized by legionellae and provide the intracellular environment required for the replication of this bacterium. In low-nutrient environments, however, L. pneumophila is able to enter a non-replicative viable but nonculturable (VBNC) state. In this study, L. pneumophila Philadelphia I JR 32 was suspended in sterilized tap water at 10(4) cells/ml. The decreasing number of bacteria was monitored by CFU measurements, acridine orange direct count (AODC), and hybridization with 16S rRNA-targeted oligonucleotide probes. After 125 days of incubation in water, the cells were no longer culturable on routine plating media; however, they were still detectable by AODC and by in situ hybridization. The addition of Acanthamoeba castellanii to the dormant bacteria resulted in the resuscitation of L. pneumophila JR 32 to a culturable state. A comparison of plate-gro...
Frontiers in microbiology, 2018
Asymptomatic bacterial colonization of the urinary bladder (asymptomatic bacteriuria, ABU) can pr... more Asymptomatic bacterial colonization of the urinary bladder (asymptomatic bacteriuria, ABU) can prevent bladder colonization by uropathogens and thus symptomatic urinary tract infection (UTI). Deliberate bladder colonization withABU isolate 83972 has been shown to outcompete uropathogens and prevent symptomatic UTI by bacterial interference. Many ABU isolates evolved from uropathogenic ancestors and, although attenuated, may still be able to express virulence-associated factors. Our aim was to screen for efficient and safe candidate strains that could be used as alternatives to83972 for preventive and therapeutic bladder colonization. To identify ABUstrains with minimal virulence potential but maximal interference efficiency, we compared nine ABU isolates from diabetic patients regarding their virulence- and fitness-associated phenotypes, their virulence in a murine model of sepsis and their genome content. We identified strains in competitive growth experiments, which successfully i...
Journal of Bacteriology, 1991
Novel fimbriae were isolated and purified from the human enteropathogen Salmonella enteritidis 27... more Novel fimbriae were isolated and purified from the human enteropathogen Salmonella enteritidis 27655. These fimbriae were thin (measuring 3 to 4 nm in diameter), were extremely aggregative, and remained cell associated despite attempts to separate them from blended cells by centrifugation. The thin fimbriae were not solubilized in 5 M NaOH or in boiling 0.5% deoxycholate, 8 M urea, or 1 to 2% sodium dodecyl sulfate (SDS) with or without 5% beta-mercaptoethanol. Therefore, an unconventional purification procedure based on the removal of contaminating cell macromolecules in sonicated cell extracts by enzymatic digestion and preparative SDS-polyacrylamide gel electrophoresis (PAGE) was used. The insoluble fimbriae recovered from the well of the gel required depolymerization in formic acid prior to analysis by SDS-PAGE. Acid depolymerization revealed that the fimbriae were composed of fimbrin subunits, each with an apparent molecular mass of 17 kDa. Although their biochemical characteri...
Advances in Experimental Medicine and Biology, 2002
... 3. Blum, G., Ott, M., Lischewski, A., Ritter, A., Imrich, H., Tschape, H., and Hacker, J., 19... more ... 3. Blum, G., Ott, M., Lischewski, A., Ritter, A., Imrich, H., Tschape, H., and Hacker, J., 1994, Excision of large DNA region termed ... 6. Oswald, E., personal communication 7. Le Bouguenec, C., Archaumband, M., and Labigne, A., 1992, Rapid and specific detection ofpap, afa, and ...
Advances in Experimental Medicine and Biology
Escherichia coli is by far the most common causative agent of urinary tract infections. The abili... more Escherichia coli is by far the most common causative agent of urinary tract infections. The ability of uropathogenic strains to produce certain virulence factors distinguishes them from commensal and intestinal pathogenic strains. Pathogenicity factors, such as fimbriae, ferric uptake ...