Lewis Johnson - Academia.edu (original) (raw)
Papers by Lewis Johnson
Film-Philosophy, 1999
Jan-Christopher Horak _Making Images Move: Photographers and Avant-Garde Cinema_ Washington and L... more Jan-Christopher Horak _Making Images Move: Photographers and Avant-Garde Cinema_ Washington and London: Smithsonian Institution Press, 1997 1-56098-744-8 297 pages
The chapter is a relatively brief overview and guide to the very-complicated endeavor of sampling... more The chapter is a relatively brief overview and guide to the very-complicated endeavor of sampling and analysis of hazardous waste and related products. Stack sampling and analysis of waste combustion products is emphasized partly due to the authors' backgrounds and partly due to the relatively recent development of most of the technology.
Proceedings of the International Pig Veterinary Society 6th Congress Copenhagen June 30th July 3rd 1980, 1980
This report was prepared as an account of work sponsored by an agency of the United States Govern... more This report was prepared as an account of work sponsored by an agency of the United States Government. Neither the United States Government nor any agency thereof, nor any of their employees, makes any wat'ranty, express or implied, or assumes any legal liability or responsibility for the accuracy, completeness, or usefulness of any information, apparatus, product, or pi, :ss disclosed, or represents that its use would not infringe privately owned rights. Rcfcr-cn_ herein to any specific commercial product, process,or service by trade name, trademark, manufacturer, or otherwise dots not necessarily constitute or imply its endorsement, recommendation, or favoring by the United States Government or any agency thereof. The views and opinions of authors expressed herein do not nlwzssarily state or reflect those of the United States Government or any agency thereof.
American Journal of Infection Control, 2014
protection was the lack of accessibility 53%; not a priority in an emergency (47%) or not necessa... more protection was the lack of accessibility 53%; not a priority in an emergency (47%) or not necessary (27%). 53% reported that face protection is available in or just outside the patient’s room. 70% wanted it available in or just outside the room. 37% reported they would use it if it were more accessible and 35% reported being in a situation inwhich they regretted not wearing face protectionwhile suctioning. LESSON LEARNED: Access to face protection supplies is inconsistent within our hospital. We learned we need to identify standard locations to store masks and eye protection, as improved accessibility may increase use by staff. Most staff would like face protection to be available either inside or directly outside of the room. Continuing education is needed regarding situations in which face protection is indicated for standard precautions.
Journal of Vacuum Science & Technology B: Microelectronics and Nanometer Structures, 1998
Extreme ultraviolet lithography (EUVL) is a promising technology for integrated circuit fabricati... more Extreme ultraviolet lithography (EUVL) is a promising technology for integrated circuit fabrication for feature sizes less than 0.1 μm. It is an optical projection lithography scheme using 13 nm radiation with all-reflective optics based on Mo/Si or Mo/Be multilayer coatings. An EUVL ...
2014 IEEE International Conference on Big Data (Big Data), 2014
Mike Barley Cristiano Castelfranchi Frank Dignum Boi Faltings Klaus Fischer Maria Gini Fausto Giu... more Mike Barley Cristiano Castelfranchi Frank Dignum Boi Faltings Klaus Fischer Maria Gini Fausto Giunchiglia Chun-Nan Hsu Lewis Johnson Jeff Kephart Kazuhiro Kuwabara Yves Lespérance Victor Lesser Jyi-shane Liu Michael Luck Stacy Marsella John-Jules Meyer Jörg Müller Pablo Noriega BV Eugenio Oliveira Lin Padgham David Parkes H. Van Dyke Parunak Omer Rana Ken Satoh Onn Shehory Jaime Sichman Reid Simmons Von-Wun Soo Leon Sterling Peter Stone Katia Sycara Daniel Thalmann Wiebe van der Hoek Gerhard Weiss Michael Wooldridge ...
Urology, 2015
OBJECTIVE To evaluate the role of stone size in predicting urinary calculus composition using Hou... more OBJECTIVE To evaluate the role of stone size in predicting urinary calculus composition using Hounsfield units on noncontrasted computed tomography (CT) scan. METHODS A retrospective review was performed for all patients who underwent ureteroscopy or percutaneous nephrolithotomy during a 1-year period, had a stone analysis performed, and had CT imaging available for review. All CT scans were reviewed by a board-certified radiologist. Variables evaluated included age, sex, body mass index, stone size, stone location, Hounsfield units (HUs), and stone composition. RESULTS We identified a total of 91 patients (41 men and 50 women) with CT imaging and stone analysis available for review. Stone analysis showed 41 calcium oxalate monohydrate (CaOxMH), 13 calcium oxalate dihydrate, 29 calcium phosphate, 5 uric acid, 2 struvite, and 1 cystine stone. Average age was 46 years, and average body mass index was 32 kg/m 2. Measured HUs varied significantly with size for CaOxMH and calcium oxalate dihydrate stones (P values <.05), but not for calcium phosphate stones (P ¼ .126). Using a CaOxMH identification value of 700-1000 HUs, 28 of 41 stone compositions (68%) would not have been correctly identified, including all 10 (100%) small (<5 mm) stones, 13 of 22 (59%) medium (5-10 mm) stones, and 5 of 9 large (>10 mm) stones (55%). CONCLUSION For calcium stones, the ability of CT HUs to predict stone composition was limited, likely due to the mixed stone composition. Within a cohort of CaOxMH stone formers, measured HUs varied linearly with stone size. All stones <5 mm were below thresholds for CaOxMH composition. UROLOGY 85: 292e295, 2015.
Journal of Vacuum Science & Technology B: Microelectronics and Nanometer Structures, 2000
Short wavelength x-ray radiation microscopy is well suited for a number of material and life scie... more Short wavelength x-ray radiation microscopy is well suited for a number of material and life science studies. The x-ray microscope ͑XM1͒ at the Advanced Light Source Synchrotron in Berkeley, California uses two diffractive Fresnel zone plate lenses. The first is a large condenser lens, which collects soft x-ray radiation from a bending magnet, focuses it, and serves as a linear monochromator. The second is the objective zone plate lens, which magnifies the image of the specimen onto a high-efficiency charge coupled device detector. The objective lens determines the numerical aperture and ultimate resolution. New objective lens zone plates with a minimum linewidth of 25 nm and excellent linewidth control have been fabricated using Berkeley Lab's 100 keV Nanowriter electron beam lithography tool, a calixarene high-resolution negative resist, and gold electroplating. Although the condenser zone plate is less critical to the resolution of the instrument, its efficiency determines the flux on the sample and ultimately the exposure time. A new condenser zone plate was fabricated and has a 9 mm diameter, 44 000 zones, and a minimum zone width of 54 nm ͑optimally the condenser and objective should have the same zone width͒. It is also fabricated with the Nanowriter at 100 keV using poly͑methylmethacrylate͒ resist and nickel electroplating. The phase shift through the nickel absorber material enhances the diffraction efficiency over an amplitude only zone plate. To evaluate the microscope's performance transmission test patterns have been made and imaged. Lineout data show modulation for 30 nm lines and 60 ͑1:2͒ spaces to be almost 100%. These new diffractive optical elements represent a significant advancement in the field of high-resolution soft x-ray microscopy. Diffractive optical elements have been used to measure the wave front error of an extreme ultraviolet projection optical system. The reference wave is generated by the spherical wave generated by diffraction from a small freestanding pinhole.
Journal of Vacuum Science & Technology B: Microelectronics and Nanometer Structures, 1999
We present recent experimental results from a prototype actinic ͑operates at the 13 nm extreme ul... more We present recent experimental results from a prototype actinic ͑operates at the 13 nm extreme ultraviolet wavelength͒ defect inspection system for extreme ultraviolet lithography mask blanks. The defect sensitivity of the current actinic inspection system is shown to reach 100 nm in experiments with programmed defects. A method to cross register and cross correlate between the actinic inspection system and a commercial visible-light scattering defect inspection system is also demonstrated. Thus, random, native defects identified using the visible-light tool can reliably be found and scanned by our actinic tool. We found that native defects as small as 86 nm ͑as classified by the visible-light tool͒ were detectable by the actinic tool. These results demonstrate the capability of this tool for independent defect counting experiments.
Two variants of photon-based soft x-ray microscopy using zone plate lenses (scanning and imaging)... more Two variants of photon-based soft x-ray microscopy using zone plate lenses (scanning and imaging) offer complementary and possibly unique capabilities to non-destructively image magnetic nanostructures compared to other magnetic imaging techniques. These capabilities include good spatial resolution (20-30 nm), element-specificity and penetrating power to study interactions between magnetic layers, field-dependent imaging capabilities, and direct quantitative sensitivity to vector magnetization. Studies using existing x-ray microscopes at LBNL's Advanced Light Source are looking at magnetic interactions between coupled magnetic layers, remnant and field-dependent magnetization in MRAM elements, vector magnetization in demagnetized films, and domain structures in films with perpendicular anisotropy. Microscopes and techniques will be reviewed, along with results from one or more of these studies. Growing experience points to limitations of existing microscopes and the need for a ...
Virchows Archiv A Pathological Anatomy and Histopathology, 1988
Mollicutes are cell wall deficient bacteria which may be overlooked or confused with viruses beca... more Mollicutes are cell wall deficient bacteria which may be overlooked or confused with viruses because of indistinct light microscopic morphology, poor staining, difficulty in cultivation, and the ability to pass 0.450 micron filters. As they have a distinctive ultrastructural appearance they can be identified using transmission electron microscopy [TEM]. Using TEM vitreous leucocytes from chronic endogenous uveitis patients may demonstrate non-cultivable intracellular 0.005-1.0 micron mollicute-like organisms [MLO], some of which develop into distinctive cell walled cocci, 0.5-0.7 micron in diameter. Inoculation of those MLO containing human vitreous into mouse eyelids produces chronic cardiac and uveal vasculitis with orbital inflammation. Similar MLO are found within the mouse lesional leucocytes. This report describes the chronic orbital inflammation with vasculitis in 67 of 100 of those MLO inoculated mice versus 0 of 200 controls (P less than 0.05). Exophthalmos with inflammation also occurred in 12 of those 67 mice (P less than 0.05). MLO were found within orbital lesional leucocytes of 10 of 10 of those mice using a TEM versus 0 of 10 controls. The results indicate that vasculitis and exophthalmos were important features of this MLO induced mouse orbital inflammation. The implication of these results for human idiopathic chronic orbital inflammatory disease is discussed.
Journal of Money, Credit and Banking, 1977
LEWIS JOHNSON* Keynesian Dynamics and Growth 1. INTRODUCTION Central to Keynes's indictment ... more LEWIS JOHNSON* Keynesian Dynamics and Growth 1. INTRODUCTION Central to Keynes's indictment of classical economic analy-sis is the fundamental Keynesian proposition: a monetary economy will not in general tend towards full employment unaided by government policy. ...
Journal of Econometrics, 1981
Journal of Chemical Education, 1979
International Journal of Human-Computer Studies, 1998
In multi-agent environments, the task of agent tracking (i.e., tracking other agents' mental stat... more In multi-agent environments, the task of agent tracking (i.e., tracking other agents' mental states) increases in difficulty when a tracker (tracking agent) only has an imperfect model of the trackee (tracked agent). Such model imperfections arise in many realworld situations, where a tracker faces resource constraints and imperfect information, and the trackees themselves modify their behaviors dynamically. While such model imperfections are unavoidable, a tracker must nonetheless attempt to be adaptive in its agent tracking. In this paper, we analyze some key issues in adaptive agent tracking, and describe an initial approach based on discrimination-based learning. The main idea is to identify the deficiency of a model based on prediction failures, and revise the model by using features that are critical in discriminating successful and failed episodes. Our preliminary experiments in simulated air-to-air combat environments have shown some promising results but many problems remain open for future research.
IEEE Intelligent Systems, 2007
Endocrinology, 1978
We have established a double antibody RIA using a rabbit antiserum prepared against reduced, carb... more We have established a double antibody RIA using a rabbit antiserum prepared against reduced, carboxymethylated (RCXM) human LH alpha-subunit, with RCXM-alpha as tracer and standard. This antiserum did not cross-react with any native gonadotropins or subunit, and reacted only weakly with RCXM-alpha. A tryptic digest of RCXM alpha-subunit was completely reactive, while chymotryptic digestion abolished all immunoreactivity. By testing with separate tryptic fragments, the recognition site could be localized to a segment close to the amino-terminus of the peptide chain. When applied to measurement of serum and urine, an immunoreactive species, parallel to RCXM alpha-subunit by serial dilution, was found in concentrations of 1-2 ng/ml in serum and 3-4 ng/ml in urine. Similar levels of the immunoreactive component were found in conditions of elevated gonadotropins (e.g. pregnancy) as well as gonadotropin deficiency(panhypopituitarism and Kallmann's syndrome). After stimulation with LHRH, no rise was noted at times up to 6 h despite the fact that both LH and LH-alpha were elevated. The data indicate that the sequence-specific antiserum may be detecting an immunoreactive form of alpha-subunit of LH whose kinetics of appearance and disappearance differs from those of the native subunit.
Film-Philosophy, 1999
Jan-Christopher Horak _Making Images Move: Photographers and Avant-Garde Cinema_ Washington and L... more Jan-Christopher Horak _Making Images Move: Photographers and Avant-Garde Cinema_ Washington and London: Smithsonian Institution Press, 1997 1-56098-744-8 297 pages
The chapter is a relatively brief overview and guide to the very-complicated endeavor of sampling... more The chapter is a relatively brief overview and guide to the very-complicated endeavor of sampling and analysis of hazardous waste and related products. Stack sampling and analysis of waste combustion products is emphasized partly due to the authors' backgrounds and partly due to the relatively recent development of most of the technology.
Proceedings of the International Pig Veterinary Society 6th Congress Copenhagen June 30th July 3rd 1980, 1980
This report was prepared as an account of work sponsored by an agency of the United States Govern... more This report was prepared as an account of work sponsored by an agency of the United States Government. Neither the United States Government nor any agency thereof, nor any of their employees, makes any wat'ranty, express or implied, or assumes any legal liability or responsibility for the accuracy, completeness, or usefulness of any information, apparatus, product, or pi, :ss disclosed, or represents that its use would not infringe privately owned rights. Rcfcr-cn_ herein to any specific commercial product, process,or service by trade name, trademark, manufacturer, or otherwise dots not necessarily constitute or imply its endorsement, recommendation, or favoring by the United States Government or any agency thereof. The views and opinions of authors expressed herein do not nlwzssarily state or reflect those of the United States Government or any agency thereof.
American Journal of Infection Control, 2014
protection was the lack of accessibility 53%; not a priority in an emergency (47%) or not necessa... more protection was the lack of accessibility 53%; not a priority in an emergency (47%) or not necessary (27%). 53% reported that face protection is available in or just outside the patient’s room. 70% wanted it available in or just outside the room. 37% reported they would use it if it were more accessible and 35% reported being in a situation inwhich they regretted not wearing face protectionwhile suctioning. LESSON LEARNED: Access to face protection supplies is inconsistent within our hospital. We learned we need to identify standard locations to store masks and eye protection, as improved accessibility may increase use by staff. Most staff would like face protection to be available either inside or directly outside of the room. Continuing education is needed regarding situations in which face protection is indicated for standard precautions.
Journal of Vacuum Science & Technology B: Microelectronics and Nanometer Structures, 1998
Extreme ultraviolet lithography (EUVL) is a promising technology for integrated circuit fabricati... more Extreme ultraviolet lithography (EUVL) is a promising technology for integrated circuit fabrication for feature sizes less than 0.1 μm. It is an optical projection lithography scheme using 13 nm radiation with all-reflective optics based on Mo/Si or Mo/Be multilayer coatings. An EUVL ...
2014 IEEE International Conference on Big Data (Big Data), 2014
Mike Barley Cristiano Castelfranchi Frank Dignum Boi Faltings Klaus Fischer Maria Gini Fausto Giu... more Mike Barley Cristiano Castelfranchi Frank Dignum Boi Faltings Klaus Fischer Maria Gini Fausto Giunchiglia Chun-Nan Hsu Lewis Johnson Jeff Kephart Kazuhiro Kuwabara Yves Lespérance Victor Lesser Jyi-shane Liu Michael Luck Stacy Marsella John-Jules Meyer Jörg Müller Pablo Noriega BV Eugenio Oliveira Lin Padgham David Parkes H. Van Dyke Parunak Omer Rana Ken Satoh Onn Shehory Jaime Sichman Reid Simmons Von-Wun Soo Leon Sterling Peter Stone Katia Sycara Daniel Thalmann Wiebe van der Hoek Gerhard Weiss Michael Wooldridge ...
Urology, 2015
OBJECTIVE To evaluate the role of stone size in predicting urinary calculus composition using Hou... more OBJECTIVE To evaluate the role of stone size in predicting urinary calculus composition using Hounsfield units on noncontrasted computed tomography (CT) scan. METHODS A retrospective review was performed for all patients who underwent ureteroscopy or percutaneous nephrolithotomy during a 1-year period, had a stone analysis performed, and had CT imaging available for review. All CT scans were reviewed by a board-certified radiologist. Variables evaluated included age, sex, body mass index, stone size, stone location, Hounsfield units (HUs), and stone composition. RESULTS We identified a total of 91 patients (41 men and 50 women) with CT imaging and stone analysis available for review. Stone analysis showed 41 calcium oxalate monohydrate (CaOxMH), 13 calcium oxalate dihydrate, 29 calcium phosphate, 5 uric acid, 2 struvite, and 1 cystine stone. Average age was 46 years, and average body mass index was 32 kg/m 2. Measured HUs varied significantly with size for CaOxMH and calcium oxalate dihydrate stones (P values <.05), but not for calcium phosphate stones (P ¼ .126). Using a CaOxMH identification value of 700-1000 HUs, 28 of 41 stone compositions (68%) would not have been correctly identified, including all 10 (100%) small (<5 mm) stones, 13 of 22 (59%) medium (5-10 mm) stones, and 5 of 9 large (>10 mm) stones (55%). CONCLUSION For calcium stones, the ability of CT HUs to predict stone composition was limited, likely due to the mixed stone composition. Within a cohort of CaOxMH stone formers, measured HUs varied linearly with stone size. All stones <5 mm were below thresholds for CaOxMH composition. UROLOGY 85: 292e295, 2015.
Journal of Vacuum Science & Technology B: Microelectronics and Nanometer Structures, 2000
Short wavelength x-ray radiation microscopy is well suited for a number of material and life scie... more Short wavelength x-ray radiation microscopy is well suited for a number of material and life science studies. The x-ray microscope ͑XM1͒ at the Advanced Light Source Synchrotron in Berkeley, California uses two diffractive Fresnel zone plate lenses. The first is a large condenser lens, which collects soft x-ray radiation from a bending magnet, focuses it, and serves as a linear monochromator. The second is the objective zone plate lens, which magnifies the image of the specimen onto a high-efficiency charge coupled device detector. The objective lens determines the numerical aperture and ultimate resolution. New objective lens zone plates with a minimum linewidth of 25 nm and excellent linewidth control have been fabricated using Berkeley Lab's 100 keV Nanowriter electron beam lithography tool, a calixarene high-resolution negative resist, and gold electroplating. Although the condenser zone plate is less critical to the resolution of the instrument, its efficiency determines the flux on the sample and ultimately the exposure time. A new condenser zone plate was fabricated and has a 9 mm diameter, 44 000 zones, and a minimum zone width of 54 nm ͑optimally the condenser and objective should have the same zone width͒. It is also fabricated with the Nanowriter at 100 keV using poly͑methylmethacrylate͒ resist and nickel electroplating. The phase shift through the nickel absorber material enhances the diffraction efficiency over an amplitude only zone plate. To evaluate the microscope's performance transmission test patterns have been made and imaged. Lineout data show modulation for 30 nm lines and 60 ͑1:2͒ spaces to be almost 100%. These new diffractive optical elements represent a significant advancement in the field of high-resolution soft x-ray microscopy. Diffractive optical elements have been used to measure the wave front error of an extreme ultraviolet projection optical system. The reference wave is generated by the spherical wave generated by diffraction from a small freestanding pinhole.
Journal of Vacuum Science & Technology B: Microelectronics and Nanometer Structures, 1999
We present recent experimental results from a prototype actinic ͑operates at the 13 nm extreme ul... more We present recent experimental results from a prototype actinic ͑operates at the 13 nm extreme ultraviolet wavelength͒ defect inspection system for extreme ultraviolet lithography mask blanks. The defect sensitivity of the current actinic inspection system is shown to reach 100 nm in experiments with programmed defects. A method to cross register and cross correlate between the actinic inspection system and a commercial visible-light scattering defect inspection system is also demonstrated. Thus, random, native defects identified using the visible-light tool can reliably be found and scanned by our actinic tool. We found that native defects as small as 86 nm ͑as classified by the visible-light tool͒ were detectable by the actinic tool. These results demonstrate the capability of this tool for independent defect counting experiments.
Two variants of photon-based soft x-ray microscopy using zone plate lenses (scanning and imaging)... more Two variants of photon-based soft x-ray microscopy using zone plate lenses (scanning and imaging) offer complementary and possibly unique capabilities to non-destructively image magnetic nanostructures compared to other magnetic imaging techniques. These capabilities include good spatial resolution (20-30 nm), element-specificity and penetrating power to study interactions between magnetic layers, field-dependent imaging capabilities, and direct quantitative sensitivity to vector magnetization. Studies using existing x-ray microscopes at LBNL's Advanced Light Source are looking at magnetic interactions between coupled magnetic layers, remnant and field-dependent magnetization in MRAM elements, vector magnetization in demagnetized films, and domain structures in films with perpendicular anisotropy. Microscopes and techniques will be reviewed, along with results from one or more of these studies. Growing experience points to limitations of existing microscopes and the need for a ...
Virchows Archiv A Pathological Anatomy and Histopathology, 1988
Mollicutes are cell wall deficient bacteria which may be overlooked or confused with viruses beca... more Mollicutes are cell wall deficient bacteria which may be overlooked or confused with viruses because of indistinct light microscopic morphology, poor staining, difficulty in cultivation, and the ability to pass 0.450 micron filters. As they have a distinctive ultrastructural appearance they can be identified using transmission electron microscopy [TEM]. Using TEM vitreous leucocytes from chronic endogenous uveitis patients may demonstrate non-cultivable intracellular 0.005-1.0 micron mollicute-like organisms [MLO], some of which develop into distinctive cell walled cocci, 0.5-0.7 micron in diameter. Inoculation of those MLO containing human vitreous into mouse eyelids produces chronic cardiac and uveal vasculitis with orbital inflammation. Similar MLO are found within the mouse lesional leucocytes. This report describes the chronic orbital inflammation with vasculitis in 67 of 100 of those MLO inoculated mice versus 0 of 200 controls (P less than 0.05). Exophthalmos with inflammation also occurred in 12 of those 67 mice (P less than 0.05). MLO were found within orbital lesional leucocytes of 10 of 10 of those mice using a TEM versus 0 of 10 controls. The results indicate that vasculitis and exophthalmos were important features of this MLO induced mouse orbital inflammation. The implication of these results for human idiopathic chronic orbital inflammatory disease is discussed.
Journal of Money, Credit and Banking, 1977
LEWIS JOHNSON* Keynesian Dynamics and Growth 1. INTRODUCTION Central to Keynes's indictment ... more LEWIS JOHNSON* Keynesian Dynamics and Growth 1. INTRODUCTION Central to Keynes's indictment of classical economic analy-sis is the fundamental Keynesian proposition: a monetary economy will not in general tend towards full employment unaided by government policy. ...
Journal of Econometrics, 1981
Journal of Chemical Education, 1979
International Journal of Human-Computer Studies, 1998
In multi-agent environments, the task of agent tracking (i.e., tracking other agents' mental stat... more In multi-agent environments, the task of agent tracking (i.e., tracking other agents' mental states) increases in difficulty when a tracker (tracking agent) only has an imperfect model of the trackee (tracked agent). Such model imperfections arise in many realworld situations, where a tracker faces resource constraints and imperfect information, and the trackees themselves modify their behaviors dynamically. While such model imperfections are unavoidable, a tracker must nonetheless attempt to be adaptive in its agent tracking. In this paper, we analyze some key issues in adaptive agent tracking, and describe an initial approach based on discrimination-based learning. The main idea is to identify the deficiency of a model based on prediction failures, and revise the model by using features that are critical in discriminating successful and failed episodes. Our preliminary experiments in simulated air-to-air combat environments have shown some promising results but many problems remain open for future research.
IEEE Intelligent Systems, 2007
Endocrinology, 1978
We have established a double antibody RIA using a rabbit antiserum prepared against reduced, carb... more We have established a double antibody RIA using a rabbit antiserum prepared against reduced, carboxymethylated (RCXM) human LH alpha-subunit, with RCXM-alpha as tracer and standard. This antiserum did not cross-react with any native gonadotropins or subunit, and reacted only weakly with RCXM-alpha. A tryptic digest of RCXM alpha-subunit was completely reactive, while chymotryptic digestion abolished all immunoreactivity. By testing with separate tryptic fragments, the recognition site could be localized to a segment close to the amino-terminus of the peptide chain. When applied to measurement of serum and urine, an immunoreactive species, parallel to RCXM alpha-subunit by serial dilution, was found in concentrations of 1-2 ng/ml in serum and 3-4 ng/ml in urine. Similar levels of the immunoreactive component were found in conditions of elevated gonadotropins (e.g. pregnancy) as well as gonadotropin deficiency(panhypopituitarism and Kallmann's syndrome). After stimulation with LHRH, no rise was noted at times up to 6 h despite the fact that both LH and LH-alpha were elevated. The data indicate that the sequence-specific antiserum may be detecting an immunoreactive form of alpha-subunit of LH whose kinetics of appearance and disappearance differs from those of the native subunit.