Liana Gouveia - Academia.edu (original) (raw)

Papers by Liana Gouveia

Research paper thumbnail of Results from PLS-DA modeling

<p>(A) Scores scatter plot discriminating among coinfected and monoinfected patients. (B) T... more <p>(A) Scores scatter plot discriminating among coinfected and monoinfected patients. (B) The optimal number of PLS-DA components, according to the squared correlation coefficient (R<sup>2</sup>), the predictive ability (Q<sup>2</sup>), and the accuracy of the model; the asterisk indicates the best number of components based on accuracy of the model.</p

Research paper thumbnail of Well-Defined ENENES Re and Mn Complexes and Their Application in Catalysis: The Role of Potassium tert-Butoxide

Research paper thumbnail of <sup>1</sup>H NMR spectra (PRESAT-CPMG, 300 MHz) of serum, with attribution of signals responsible for discrimination

<p><sup>1</sup>H NMR spectra (PRESAT-CPMG, 300 MHz) of serum, with attribution ... more <p><sup>1</sup>H NMR spectra (PRESAT-CPMG, 300 MHz) of serum, with attribution of signals responsible for discrimination.</p

Research paper thumbnail of Clinical and laboratory characteristics of the coinfected (n = 18) and monoinfected groups (n = 22)

<p>Clinical and laboratory characteristics of the coinfected (n = 18) and monoinfected grou... more <p>Clinical and laboratory characteristics of the coinfected (n = 18) and monoinfected groups (n = 22).</p

Research paper thumbnail of Results from PCA modeling

<p>(A) PCA scores of coinfected patients and monoinfected patients. (B) Loading scatter plo... more <p>(A) PCA scores of coinfected patients and monoinfected patients. (B) Loading scatter plot of PC1 versus PC2, explaining 80.7% of variance.</p

Research paper thumbnail of Results from PLS-DA modeling

<p>(A) Scores scatter plot discriminating among coinfected and monoinfected patients. (B) T... more <p>(A) Scores scatter plot discriminating among coinfected and monoinfected patients. (B) The optimal number of PLS-DA components, according to the squared correlation coefficient (R<sup>2</sup>), the predictive ability (Q<sup>2</sup>), and the accuracy of the model; the asterisk indicates the best number of components based on accuracy of the model.</p

Research paper thumbnail of Identificação De Alterações Na Atividade Das Enzimas Hepáticas Em Pacientes Com Infecção Crônica Pelos Vírus Da Hepatite B Ou C Por Metabonômica Baseada Em RMN De 1 H

Avanços científicos em medicina 2, 2021

O objetivo deste estudo foi identificar alterações na atividade das enzimas hepáticas (ALT, AST e... more O objetivo deste estudo foi identificar alterações na atividade das enzimas hepáticas (ALT, AST e GGT) devido à infecção pelos vírus da hepatite B (HBV) ou C (HCV), em uma única amostra de soro, por meio de modelos metabonômicos baseados em espectroscopia de Ressonância Magnética Nuclear de hidrogênio (RMN de 1 H). Foram incluídos 203 pacientes adultos previamente diagnosticados com monoinfecção pelo HBV (n=117) ou HCV (n=86). Os espectros de RMN foram obtidos utilizando o espectrômetro VARIAN Unity Plus 300, enquanto os níveis de atividade enzimática por método cinético automatizado, que foram tidos como padrão-ouro. Os modelos foram construídos usando Análise Discriminante por Projeção Ortogonal de Estruturas Latentes (OPLS-DA), na plataforma MetaboAnalyst 3.6. São três modelos para avaliação da atividade enzimática, independentemente do tipo de vírus relacionado à infecção: (1) ALT; (2) AST; e (3) GGT. O modelo ALT apresentou sensibilidade (Sn) de 95,4%, especificidade (Sp) de 93...

Research paper thumbnail of Diagnosis of coinfection by schistosomiasis and viral hepatitis B or C using 1H NMR-based metabonomics

PloS one, 2017

Diagnosis of liver involvement due to schistosomiasis in asymptomatic patients from endemic areas... more Diagnosis of liver involvement due to schistosomiasis in asymptomatic patients from endemic areas previously diagnosed with chronic hepatitis B (HBV) or C (HCV) and periportal fibrosis is challenging. H-1 Nuclear Magnetic Resonance (NMR)-based metabonomics strategy is a powerful tool for providing a profile of endogenous metabolites of low molecular weight in biofluids in a non-invasive way. The aim of this study was to diagnose periportal fibrosis due to schistosomiasis mansoni in patients with chronic HBV or HCV infection through NMR-based metabonomics models. The study included 40 patients divided into two groups: (i) 18 coinfected patients with schistosomiasis mansoni and HBV or HCV; and (ii) 22 HBV or HCV monoinfected patients. The serum samples were analyzed through H-1 NMR spectroscopy and the models were based on Principal Component Analysis (PCA) and Partial Least Squares-Discriminant Analysis (PLS-DA). Ultrasonography examination was used to ascertain the diagnosis of peri...

Research paper thumbnail of Discriminating gamma-irradiated soybean seeds by 1H NMR-based metabonomics

Food Control, 2014

Abstract Gamma irradiation has been paramount for enhancing the quality of grain production, main... more Abstract Gamma irradiation has been paramount for enhancing the quality of grain production, mainly in terms of distribution and storage. Gamma irradiation classification models for soybeans, however, are still in development. In this paper, we present a metabonomic model able to distinguish between gamma-irradiated and non-irradiated soybeans. The metabonomic model works on 1H NMR spectra of chloroform extracts and makes use of PCA and PLS-DA formalisms to classify the samples and to investigate which spectral bins are discriminatory. The model has presented an accuracy of 100% in the face of a real dataset involving 49 samples from diverse cultivars. In turn, the most important chemical shifts (δ) for discriminating among the samples were δ 1.57 and 1.62 ppm, which are assigned to β-carboxyl methylene groups of aliphatic chains of fatty acids. Besides, the gamma-irradiated samples showed an increasing in the integration areas at δ 1.57 ppm (assigned to free fatty acid) whilst non-irradiated samples showed an increasing in the integration areas at δ 1.62 ppm (assigned to fatty acids linked to glycerol as esters).

Research paper thumbnail of Metabonômica aplicada ao diagnóstico diferencial de doenças hepáticas

Metabonomics can be defined as a tool that associates analytical data and multivariate statistics... more Metabonomics can be defined as a tool that associates analytical data and multivariate statistics analysis to identify changes in the concentration of endogenous metabolites in biofluids when there is a disturbance in the organism caused by a disease, for example. 1 H Nuclear Magnetic Resonance spectroscopy (1 H-NMR) is usually the analytical instrument used in the experiments. It is also necessary to use chemometrics techniques to extract the information of the spectra. Herein, we used blood serum samples from patients of Clinics Hospital of UFPE and from three hospitals of Recife city to built metabonomics models able to: 1) distinguish between steatosis and steatohepatitis; and 2) distinguish between monoinfected patients with viral hepatitis B (HBV) or C (HCV) and coinfected patients with HBV/HCV and schistosomiasis. These models were built using PLS-DA and OPLS-DA formalisms. The first model contains 39 samples and achieved values of accuracy, R 2 , sensitivity and specificity of 81.1%, 75.0%, 71.4% and 83.3%, respectively. This result was similar to the one obtained by cytokeratin-18, an efficient noninvasive biomarker for steatohepatitis. The second model contains 40 samples and achieved values of accuracy, R 2 and Q 2 equal to 100.0%, 98.1% and 97.5%, respectively. The metabonomics strategy, a minimally invasive strategy, showed potential to assess the presence of hepatic lesions.

Research paper thumbnail of Results from PLS-DA modeling

<p>(A) Scores scatter plot discriminating among coinfected and monoinfected patients. (B) T... more <p>(A) Scores scatter plot discriminating among coinfected and monoinfected patients. (B) The optimal number of PLS-DA components, according to the squared correlation coefficient (R<sup>2</sup>), the predictive ability (Q<sup>2</sup>), and the accuracy of the model; the asterisk indicates the best number of components based on accuracy of the model.</p

Research paper thumbnail of Well-Defined ENENES Re and Mn Complexes and Their Application in Catalysis: The Role of Potassium tert-Butoxide

Research paper thumbnail of <sup>1</sup>H NMR spectra (PRESAT-CPMG, 300 MHz) of serum, with attribution of signals responsible for discrimination

<p><sup>1</sup>H NMR spectra (PRESAT-CPMG, 300 MHz) of serum, with attribution ... more <p><sup>1</sup>H NMR spectra (PRESAT-CPMG, 300 MHz) of serum, with attribution of signals responsible for discrimination.</p

Research paper thumbnail of Clinical and laboratory characteristics of the coinfected (n = 18) and monoinfected groups (n = 22)

<p>Clinical and laboratory characteristics of the coinfected (n = 18) and monoinfected grou... more <p>Clinical and laboratory characteristics of the coinfected (n = 18) and monoinfected groups (n = 22).</p

Research paper thumbnail of Results from PCA modeling

<p>(A) PCA scores of coinfected patients and monoinfected patients. (B) Loading scatter plo... more <p>(A) PCA scores of coinfected patients and monoinfected patients. (B) Loading scatter plot of PC1 versus PC2, explaining 80.7% of variance.</p

Research paper thumbnail of Results from PLS-DA modeling

<p>(A) Scores scatter plot discriminating among coinfected and monoinfected patients. (B) T... more <p>(A) Scores scatter plot discriminating among coinfected and monoinfected patients. (B) The optimal number of PLS-DA components, according to the squared correlation coefficient (R<sup>2</sup>), the predictive ability (Q<sup>2</sup>), and the accuracy of the model; the asterisk indicates the best number of components based on accuracy of the model.</p

Research paper thumbnail of Identificação De Alterações Na Atividade Das Enzimas Hepáticas Em Pacientes Com Infecção Crônica Pelos Vírus Da Hepatite B Ou C Por Metabonômica Baseada Em RMN De 1 H

Avanços científicos em medicina 2, 2021

O objetivo deste estudo foi identificar alterações na atividade das enzimas hepáticas (ALT, AST e... more O objetivo deste estudo foi identificar alterações na atividade das enzimas hepáticas (ALT, AST e GGT) devido à infecção pelos vírus da hepatite B (HBV) ou C (HCV), em uma única amostra de soro, por meio de modelos metabonômicos baseados em espectroscopia de Ressonância Magnética Nuclear de hidrogênio (RMN de 1 H). Foram incluídos 203 pacientes adultos previamente diagnosticados com monoinfecção pelo HBV (n=117) ou HCV (n=86). Os espectros de RMN foram obtidos utilizando o espectrômetro VARIAN Unity Plus 300, enquanto os níveis de atividade enzimática por método cinético automatizado, que foram tidos como padrão-ouro. Os modelos foram construídos usando Análise Discriminante por Projeção Ortogonal de Estruturas Latentes (OPLS-DA), na plataforma MetaboAnalyst 3.6. São três modelos para avaliação da atividade enzimática, independentemente do tipo de vírus relacionado à infecção: (1) ALT; (2) AST; e (3) GGT. O modelo ALT apresentou sensibilidade (Sn) de 95,4%, especificidade (Sp) de 93...

Research paper thumbnail of Diagnosis of coinfection by schistosomiasis and viral hepatitis B or C using 1H NMR-based metabonomics

PloS one, 2017

Diagnosis of liver involvement due to schistosomiasis in asymptomatic patients from endemic areas... more Diagnosis of liver involvement due to schistosomiasis in asymptomatic patients from endemic areas previously diagnosed with chronic hepatitis B (HBV) or C (HCV) and periportal fibrosis is challenging. H-1 Nuclear Magnetic Resonance (NMR)-based metabonomics strategy is a powerful tool for providing a profile of endogenous metabolites of low molecular weight in biofluids in a non-invasive way. The aim of this study was to diagnose periportal fibrosis due to schistosomiasis mansoni in patients with chronic HBV or HCV infection through NMR-based metabonomics models. The study included 40 patients divided into two groups: (i) 18 coinfected patients with schistosomiasis mansoni and HBV or HCV; and (ii) 22 HBV or HCV monoinfected patients. The serum samples were analyzed through H-1 NMR spectroscopy and the models were based on Principal Component Analysis (PCA) and Partial Least Squares-Discriminant Analysis (PLS-DA). Ultrasonography examination was used to ascertain the diagnosis of peri...

Research paper thumbnail of Discriminating gamma-irradiated soybean seeds by 1H NMR-based metabonomics

Food Control, 2014

Abstract Gamma irradiation has been paramount for enhancing the quality of grain production, main... more Abstract Gamma irradiation has been paramount for enhancing the quality of grain production, mainly in terms of distribution and storage. Gamma irradiation classification models for soybeans, however, are still in development. In this paper, we present a metabonomic model able to distinguish between gamma-irradiated and non-irradiated soybeans. The metabonomic model works on 1H NMR spectra of chloroform extracts and makes use of PCA and PLS-DA formalisms to classify the samples and to investigate which spectral bins are discriminatory. The model has presented an accuracy of 100% in the face of a real dataset involving 49 samples from diverse cultivars. In turn, the most important chemical shifts (δ) for discriminating among the samples were δ 1.57 and 1.62 ppm, which are assigned to β-carboxyl methylene groups of aliphatic chains of fatty acids. Besides, the gamma-irradiated samples showed an increasing in the integration areas at δ 1.57 ppm (assigned to free fatty acid) whilst non-irradiated samples showed an increasing in the integration areas at δ 1.62 ppm (assigned to fatty acids linked to glycerol as esters).

Research paper thumbnail of Metabonômica aplicada ao diagnóstico diferencial de doenças hepáticas

Metabonomics can be defined as a tool that associates analytical data and multivariate statistics... more Metabonomics can be defined as a tool that associates analytical data and multivariate statistics analysis to identify changes in the concentration of endogenous metabolites in biofluids when there is a disturbance in the organism caused by a disease, for example. 1 H Nuclear Magnetic Resonance spectroscopy (1 H-NMR) is usually the analytical instrument used in the experiments. It is also necessary to use chemometrics techniques to extract the information of the spectra. Herein, we used blood serum samples from patients of Clinics Hospital of UFPE and from three hospitals of Recife city to built metabonomics models able to: 1) distinguish between steatosis and steatohepatitis; and 2) distinguish between monoinfected patients with viral hepatitis B (HBV) or C (HCV) and coinfected patients with HBV/HCV and schistosomiasis. These models were built using PLS-DA and OPLS-DA formalisms. The first model contains 39 samples and achieved values of accuracy, R 2 , sensitivity and specificity of 81.1%, 75.0%, 71.4% and 83.3%, respectively. This result was similar to the one obtained by cytokeratin-18, an efficient noninvasive biomarker for steatohepatitis. The second model contains 40 samples and achieved values of accuracy, R 2 and Q 2 equal to 100.0%, 98.1% and 97.5%, respectively. The metabonomics strategy, a minimally invasive strategy, showed potential to assess the presence of hepatic lesions.