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Papers by Liliana Serwecińska

Microbial Ecology, 2015

An increased incidence of cyanobacterial blooms, which are largely composed of toxigenic cyanobac... more An increased incidence of cyanobacterial blooms, which are largely composed of toxigenic cyanobacteria from the Microcystis genus, leads to a disruption of aquatic ecosystems worldwide. Therefore, a better understanding of the impact of environmental parameters on the development and collapse of blooms is important. The objectives of the present study were as follows: (1) to investigate the presence and identity of Microcystis-specific cyanophages capable of cyanobacterial cell lysis in a lowland dam reservoir in Central Europe; (2) to investigate Microcystis sensitivity to phage infections with regard to toxic genotypes; and (3) to identify key abiotic parameters influencing phage infections during the summer seasons between 2009 and 2013. Sequencing analysis of selected g91 gene amplification products confirmed that the identified cyanophages belonged to the family Myoviridae (95 % homology). Cyanophages and Microcystis hosts, including toxic genotypes, were positively correlated in 4 of the 5 years analyzed (r=0.67-0.82). The average percentage of infected Microcystis cells varied between 0.1 and 32 %, and no particular sensitivity of the phages to toxigenic genotypes was recorded. The highest number of cyanophages (>10 4 gene copy number per microliter) was observed in the period preceded by the following: an increase of the water retention time, growth of the water temperature, optimum nutrient concentrations, and the predomination of Microcystis bloom.

Ecohydrology & Hydrobiology

Biologia

The paper describes the isolation and characterization of a novel pelagic bacterial strain capabl... more The paper describes the isolation and characterization of a novel pelagic bacterial strain capable of hepatotoxic microcystin (MC) degradation. From a total of 24 bacterial strains isolated from water samples collected during the cyanobacteria blooming period in Jeziorsko reservoir, Poland, only the strain JEZ-8L was found to completely degrade microcystin-LR (MC-LR). The strain JEZ-8L was found to include the full

Ecohydrology & Hydrobiology

Water

The release of antibiotics to the environment, and the consequences of the presence of persistent... more The release of antibiotics to the environment, and the consequences of the presence of persistent antimicrobial residues in ecosystems, have been the subject of numerous studies in all parts of the world. The overuse and misuse of antibiotics is a common global phenomenon, which substantially increases the levels of antibiotics in the environment and the rates of their spread. Today, it can be said with certainty that the mass production and use of antibiotics for purposes other than medical treatment has an impact on both the environment and human health. This review aims to track the pathways of the environmental distribution of antimicrobials and identify the biological effects of their subinhibitory concentration in different environmental compartments; it also assesses the associated public health risk and government policy interventions needed to ensure the effectiveness of existing antimicrobials. The recent surge in interest in this issue has been driven by the dramatic incr...

Scientific Reports

Novel heterotrophic bacterial strains—Bzr02 and Str21, effective in nitrogen transformation, were... more Novel heterotrophic bacterial strains—Bzr02 and Str21, effective in nitrogen transformation, were isolated from sequential sedimentation-biofiltration systems (SSBSs). Bzr02, identified as Citrobacter freundii, removed up to 99.0% of N–NH4 and 70.2% of N–NO3, while Str21, identified as Pseudomonas mandelii, removed up to 98.9% of N–NH4 and 87.7% of N–NO3. The key functional genes napA/narG and hao were detected for Bzr02, confirming its ability to reduce nitrate to nitrite and remove hydroxylamine. Str21 was detected with the genes narG, nirS, norB and nosZ, confirming its potential for complete denitrification process. Nitrogen total balance experiments determined that Bzr02 and Str21 incorporated nitrogen into cell biomass (up to 94.7% and 74.7%, respectively), suggesting that nitrogen assimilation was also an important process occurring simultaneously with denitrification. Based on these results, both strains are suitable candidates for improving nutrient removal efficiencies in ...

Science of The Total Environment

Carbohydrate Research, 2000

The N-trifluoroacetyl-and N-tetrachlorophthaloyl-protected bromide of D-glucosamine has been used... more The N-trifluoroacetyl-and N-tetrachlorophthaloyl-protected bromide of D-glucosamine has been used for the first time as a glycosyl donor for the glycosylation of diosgenin [(25R)-spirost-5-en-3b-ol]. Both 1,3,4,6-tetra-O-acetyl-2deoxy-2-trifluoroacetamido-b-D-glucopyranoside and 1,3,4,6-tetra-O-acetyl-2-deoxy-2-tetrachlorophthalimido-a,b-Dglucopyranoside were transformed into the appropriate glycosyl bromides. These reacted with diosgenin under mild conditions, using silver triflate as a promoter, and gave the corresponding protected diosgenyl glycosides. Each was deprotected to give diosgenyl 2-amino-2-deoxy-b-D-glucopyranoside hydrochloride. The structures of the new glycosides were established by 1 H NMR spectroscopy.

Carbohydrate research, Jan 22, 2000

The N-trifluoroacetyl- and N-tetrachlorophthaloyl-protected bromide of D-glucosamine has been use... more The N-trifluoroacetyl- and N-tetrachlorophthaloyl-protected bromide of D-glucosamine has been used for the first time as a glycosyl donor for the glycosylation of diosgenin [(25R)-spirost-5-en-3beta-ol]. Both 1,3,4,6-tetra-O-acetyl-2-deoxy-2-trifluoroacetamido-beta-D-glucopy ranoside and 1,3,4,6-tetra-O-acetyl-2-deoxy-2-tetrachlorophthalimido-alpha,beta -D-glucopyranoside were transformed into the appropriate glycosyl bromides. These reacted with diosgenin under mild conditions, using silver triflate as a promoter, and gave the corresponding protected diosgenyl glycosides. Each was deprotected to give diosgenyl 2-amino-2-deoxy-beta-D-glucopyranoside hydrochloride. The structures of the new glycosides were established by 1H NMR spectroscopy.

Science of The Total Environment

The aim of the study was to determine the impact of sewage sludge from three wastewater treatment... more The aim of the study was to determine the impact of sewage sludge from three wastewater treatment plants of different sizes (small, medium and large) applied in two doses (3 and 9 tons per hectare) on soil properties, determined as the content of organic carbon and humus fractions, bacterial abundance, phytotoxicity and PCDD/PCDF TEQ concentrations. The study also evaluated the impact of this sewage sludge on the biometric and physiological parameters and detoxification reaction of willow (Salix sp.) as a typical crop used for the remediation of soil following sludge application. The cultivation of willow on soil treated with sludge was found to result in a gradual increase of humus fractions, total organic carbon content and bacterial abundance as well as soil properties measured using Lepidium sativum. However, it also produced an initial increase of soil phytotoxicity, indicated by Sinapis alba and Sorghum sacharatum, and PCDD/PCDF Toxic Equivalent (TEQ) concentrations, which then fell during the course of the experiment, particularly in areas planted by willow. Although the soil phytotoxicity and PCDD/PCDF TEQ content of the sewage sludge-amended soil initially increased, sludge application was found to have a positive influence on willow, probably due to its high nutrient and carbon content. The obtained results reveal increases in willow biomass, average leaf surface area and leaf length as well as chlorophyll a+b content. Moreover, a strong decline was found in the activity of the detoxifying enzyme glutathione S-transferase (GSTs), a multifunctional enzyme involved in the metabolism of xenobiotics in plants, again demonstrating the used sludge had a positive influence on willow performance.

Microbial Ecology, 2015

An increased incidence of cyanobacterial blooms, which are largely composed of toxigenic cyanobac... more An increased incidence of cyanobacterial blooms, which are largely composed of toxigenic cyanobacteria from the Microcystis genus, leads to a disruption of aquatic ecosystems worldwide. Therefore, a better understanding of the impact of environmental parameters on the development and collapse of blooms is important. The objectives of the present study were as follows: (1) to investigate the presence and identity of Microcystis-specific cyanophages capable of cyanobacterial cell lysis in a lowland dam reservoir in Central Europe; (2) to investigate Microcystis sensitivity to phage infections with regard to toxic genotypes; and (3) to identify key abiotic parameters influencing phage infections during the summer seasons between 2009 and 2013. Sequencing analysis of selected g91 gene amplification products confirmed that the identified cyanophages belonged to the family Myoviridae (95 % homology). Cyanophages and Microcystis hosts, including toxic genotypes, were positively correlated in 4 of the 5 years analyzed (r=0.67-0.82). The average percentage of infected Microcystis cells varied between 0.1 and 32 %, and no particular sensitivity of the phages to toxigenic genotypes was recorded. The highest number of cyanophages (>10 4 gene copy number per microliter) was observed in the period preceded by the following: an increase of the water retention time, growth of the water temperature, optimum nutrient concentrations, and the predomination of Microcystis bloom.

Ecohydrology & Hydrobiology

Biologia

The paper describes the isolation and characterization of a novel pelagic bacterial strain capabl... more The paper describes the isolation and characterization of a novel pelagic bacterial strain capable of hepatotoxic microcystin (MC) degradation. From a total of 24 bacterial strains isolated from water samples collected during the cyanobacteria blooming period in Jeziorsko reservoir, Poland, only the strain JEZ-8L was found to completely degrade microcystin-LR (MC-LR). The strain JEZ-8L was found to include the full

Ecohydrology & Hydrobiology

Water

The release of antibiotics to the environment, and the consequences of the presence of persistent... more The release of antibiotics to the environment, and the consequences of the presence of persistent antimicrobial residues in ecosystems, have been the subject of numerous studies in all parts of the world. The overuse and misuse of antibiotics is a common global phenomenon, which substantially increases the levels of antibiotics in the environment and the rates of their spread. Today, it can be said with certainty that the mass production and use of antibiotics for purposes other than medical treatment has an impact on both the environment and human health. This review aims to track the pathways of the environmental distribution of antimicrobials and identify the biological effects of their subinhibitory concentration in different environmental compartments; it also assesses the associated public health risk and government policy interventions needed to ensure the effectiveness of existing antimicrobials. The recent surge in interest in this issue has been driven by the dramatic incr...

Scientific Reports

Novel heterotrophic bacterial strains—Bzr02 and Str21, effective in nitrogen transformation, were... more Novel heterotrophic bacterial strains—Bzr02 and Str21, effective in nitrogen transformation, were isolated from sequential sedimentation-biofiltration systems (SSBSs). Bzr02, identified as Citrobacter freundii, removed up to 99.0% of N–NH4 and 70.2% of N–NO3, while Str21, identified as Pseudomonas mandelii, removed up to 98.9% of N–NH4 and 87.7% of N–NO3. The key functional genes napA/narG and hao were detected for Bzr02, confirming its ability to reduce nitrate to nitrite and remove hydroxylamine. Str21 was detected with the genes narG, nirS, norB and nosZ, confirming its potential for complete denitrification process. Nitrogen total balance experiments determined that Bzr02 and Str21 incorporated nitrogen into cell biomass (up to 94.7% and 74.7%, respectively), suggesting that nitrogen assimilation was also an important process occurring simultaneously with denitrification. Based on these results, both strains are suitable candidates for improving nutrient removal efficiencies in ...

Science of The Total Environment

Carbohydrate Research, 2000

The N-trifluoroacetyl-and N-tetrachlorophthaloyl-protected bromide of D-glucosamine has been used... more The N-trifluoroacetyl-and N-tetrachlorophthaloyl-protected bromide of D-glucosamine has been used for the first time as a glycosyl donor for the glycosylation of diosgenin [(25R)-spirost-5-en-3b-ol]. Both 1,3,4,6-tetra-O-acetyl-2deoxy-2-trifluoroacetamido-b-D-glucopyranoside and 1,3,4,6-tetra-O-acetyl-2-deoxy-2-tetrachlorophthalimido-a,b-Dglucopyranoside were transformed into the appropriate glycosyl bromides. These reacted with diosgenin under mild conditions, using silver triflate as a promoter, and gave the corresponding protected diosgenyl glycosides. Each was deprotected to give diosgenyl 2-amino-2-deoxy-b-D-glucopyranoside hydrochloride. The structures of the new glycosides were established by 1 H NMR spectroscopy.

Carbohydrate research, Jan 22, 2000

The N-trifluoroacetyl- and N-tetrachlorophthaloyl-protected bromide of D-glucosamine has been use... more The N-trifluoroacetyl- and N-tetrachlorophthaloyl-protected bromide of D-glucosamine has been used for the first time as a glycosyl donor for the glycosylation of diosgenin [(25R)-spirost-5-en-3beta-ol]. Both 1,3,4,6-tetra-O-acetyl-2-deoxy-2-trifluoroacetamido-beta-D-glucopy ranoside and 1,3,4,6-tetra-O-acetyl-2-deoxy-2-tetrachlorophthalimido-alpha,beta -D-glucopyranoside were transformed into the appropriate glycosyl bromides. These reacted with diosgenin under mild conditions, using silver triflate as a promoter, and gave the corresponding protected diosgenyl glycosides. Each was deprotected to give diosgenyl 2-amino-2-deoxy-beta-D-glucopyranoside hydrochloride. The structures of the new glycosides were established by 1H NMR spectroscopy.

Science of The Total Environment

The aim of the study was to determine the impact of sewage sludge from three wastewater treatment... more The aim of the study was to determine the impact of sewage sludge from three wastewater treatment plants of different sizes (small, medium and large) applied in two doses (3 and 9 tons per hectare) on soil properties, determined as the content of organic carbon and humus fractions, bacterial abundance, phytotoxicity and PCDD/PCDF TEQ concentrations. The study also evaluated the impact of this sewage sludge on the biometric and physiological parameters and detoxification reaction of willow (Salix sp.) as a typical crop used for the remediation of soil following sludge application. The cultivation of willow on soil treated with sludge was found to result in a gradual increase of humus fractions, total organic carbon content and bacterial abundance as well as soil properties measured using Lepidium sativum. However, it also produced an initial increase of soil phytotoxicity, indicated by Sinapis alba and Sorghum sacharatum, and PCDD/PCDF Toxic Equivalent (TEQ) concentrations, which then fell during the course of the experiment, particularly in areas planted by willow. Although the soil phytotoxicity and PCDD/PCDF TEQ content of the sewage sludge-amended soil initially increased, sludge application was found to have a positive influence on willow, probably due to its high nutrient and carbon content. The obtained results reveal increases in willow biomass, average leaf surface area and leaf length as well as chlorophyll a+b content. Moreover, a strong decline was found in the activity of the detoxifying enzyme glutathione S-transferase (GSTs), a multifunctional enzyme involved in the metabolism of xenobiotics in plants, again demonstrating the used sludge had a positive influence on willow performance.