Lina Matera - Academia.edu (original) (raw)

Papers by Lina Matera

Research paper thumbnail of Influence of drug‐induced apoptotic death on processing and presentation of tumor antigens by dendritic cells

… journal of cancer, 2003

Here we have studied the effects of apoptotic cell death induced by chemotherapic agents on tumor... more Here we have studied the effects of apoptotic cell death induced by chemotherapic agents on tumor phagocytosis by dendritic cells (DC) and presentation of the relevant antigen to T lymphocytes. Annexin-V-FITC (Ann-V) and propidium iodide (PI) staining was used to assess early apoptotic (Ann-V ؉ /PI ؊ ) vs. late apoptotic/secondary necrotic (Ann-V ؉ /PI ؉ ) death after a 24 hr observation of untreated and drug-treated gastric carcinoma cells. After treatments, the HLA-A*0201 ؉ tumor cell line KATO III was exposed for 24 hr to allogeneic, HLA-related GM-CSF, IL-4-driven immature (i) DC. Tumorloaded iDC were tested for IL-12 release in an ELISA assay, incubated with the DC-maturating factor TNF-␣ and used as stimulators for autologous T lymphocytes.

Research paper thumbnail of A Late Quaternary canyon/channel system, northwest Gulf of Mexico continental slope

Abstract A late Quaternary submarine canyon and channel system, mapped on the north-west slope (l... more Abstract A late Quaternary submarine canyon and channel system, mapped on the north-west slope (long. 94–95 W) of the Gulf of Mexico with high-resolution (3.5 kHz) seismic reflection profiling and sidescan sonar, can be divided into two types of units:(1) leveed, ...

Research paper thumbnail of Prolactin is an autocrine growth factor for the Jurkat human T-leukemic cell line

Despite convincing evidence of cooperation between IL-2 and endogenous prolactin (PRL) during T c... more Despite convincing evidence of cooperation between IL-2 and endogenous prolactin (PRL) during T cell activation, the individual role of PRL as a T-cell lineage cytokine remains to be defined. We have examined the production and function of PRL on the Jurkat human T-leukemic cell line, which does not constitutively produce IL-2. The majority of Jurkat cells expressed PRL receptor (R) under standard culture conditions, whereas appearance of the alpha chain of the IL-2-R required PHA-PMA stimulation, as did IL-2 synthesis. Western blotting revealed a predominant band at 23.5 kDa and a weaker band at 25.5 kDa in both Jurkat cell lysates and human (h) pituitary PRL. Metabolic labeling of the cell lysates with 35S-methionine and immunoprecipitation with an antiserum against hPRL showed that both forms of PRL are actively synthesized by the Jurkat cell line. PRL released in the medium was biologically active in the rat Nb2 lymphoma mitogenic assay. Depletion of medium PRL with two polyclonal anti-hPRL antisera inhibited the growth of Jurkat cells in a dose-dependent manner, as evaluated by cell number and 3H-TdR uptake. Purified pituitary or recombinant hPRL at a wide range of concentrations had no significant effect on their growth, but reversed the blocking activity of the anti-hPRL antibody. Recombinant IL-2 had no effect on the antibody-induced growth inhibition. Taken as a whole, these results demonstrate that PRL can act as an autocrine T cell growth factor independently of IL-2 and are the first evidence of its involvement in human leukemic growth and possibly in leukemic transformation.

Research paper thumbnail of Endocrine, paracrine and autocrine actions of prolactin on immune cells

The immune response is regulated by locally released factors, collectively referred to as cytokin... more The immune response is regulated by locally released factors, collectively referred to as cytokines. Data on the human immune system have convincingly demonstrated that the hormone prolactin (PRL), in addition to exerting its endocrine control on the immune system, acts as a cytokine in that it is released within the immune system and regulates the lymphocyte response by paracrine and autocrine mechanisms. Both lymphocyte and pituitary PRLs are under the control of immune factors. Synthesis of human PRL by lymphocytes is induced by T-cell stimuli, while increased release of PRL by the pituitary, observed in vivo after immune challenge, may be mediated by cytokines produced by monocyte-macrophages. Since hyperprolactinemia and hypoprolactinemia are both immunosuppressive, physiological levels of circulating PRL must be necessary to maintain basal immunocompetence. The effects of Cyclosporin (CsA) on IL-2 and PRL gene activation and the analysis of the intracellular signaling events downstream IL-2 and PRL receptors suggest coordinate actions of these two cytokines during T cell activation.

Research paper thumbnail of Platelet-Activating Factor-Stimulated In Vitro Erythropoiesis Bone Marrow Stroma-Derived Prolactin Is Involved in Basal and

Research paper thumbnail of In vitro maturation of dendritic cells from blood progenitors

Methods in molecular biology (Clifton, N.J.), 2003

Research paper thumbnail of Effect of two aliphatic aldehydes, methylglyoxal and 4-hydroxypentenal, on the growth of Yoshida ascites hepatoma AH-130

The influence of a ketoaldehyde, methylglyoxal (MG), and a hydroxyalkenal, 4-hydroxypentenal (HPE... more The influence of a ketoaldehyde, methylglyoxal (MG), and a hydroxyalkenal, 4-hydroxypentenal (HPE), on the growth of a highly-deviated turnout has been investigated. MG and HPE, administered intraperitoneally, strongly depressed in rats the proliferative activity of the Yoshida ascites hepatoma AH-130, reducing its mitotic and labelling indices as well as the proportion of cycling cells (growth fraction). Monitoring the effects on the cell cycle by the labelled mitoses method showed that the percentage of labelled mitoses was markedly lowered after either aldehyde, which is indicative for a blocking effect in the S phase. In addition, the mean cell cycle time was slightly prolonged by MG, probably due to accumulation of cells in G1, whereas HPE delayed the first mitotic peak and increased the mean DNA synthetic period without modifying the overall cycle time. The effects of HPE on the cell cycle were prevented by pretreatment with polyamines. Bvpw~cd do~c~ of MG ~ignlflcantly increased the fraction of tumour-bearlng rats surviving at 90 days ('indefinite' survivors) as well as the survival time of those which succumbed, implying that the carcinostatic effect of MG persisted over several cell cycles. By contrast, HPE did not significantly modify the survival of AH-130-bearing rats, suggesting that its influence on tumour growth was rapidly reversible.

Research paper thumbnail of Immunogenicity of 56° C and UVC‐treated prostate cancer is associated with release of HSP70 and HMGB1 from necrotic cells

The Prostate, 2009

BACKGROUND Prostate hyperthermia and photodynamic therapy can be delivered by a variety of proced... more BACKGROUND Prostate hyperthermia and photodynamic therapy can be delivered by a variety of procedures which result in a wide range of temperatures and light energy and cause different kinds of cell death. METHODS We have addressed the immunogenic effect ...

Research paper thumbnail of Increased expression of HSP70 by colon cancer cells is not always associated with access to the dendritic cell cross-presentation pathway

Cellular & Molecular …, 2007

Abstract: Dendritic cells (DCs) are highly specialized antigen-presenting cells endowed with the ... more Abstract: Dendritic cells (DCs) are highly specialized antigen-presenting cells endowed with the unique ability to not only present exogenous antigens upon exposure to MHC II, but also to cross-present these upon exposure to MHC I. This property was exploited to generate ...

Research paper thumbnail of Prostatectomy restores the maturation competence of blood dendritic cell precursors and reverses the abnormal expansion of regulatory T lymphocytes

The …, 2010

Despite loss of the monocytes marker CD14, cytokine-matured DCs of tumor bearing patients express... more Despite loss of the monocytes marker CD14, cytokine-matured DCs of tumor bearing patients expressed lower levels of the costimulatory molecule CD80 and of the maturation markers CD83 and CCR7 compared to mDC of normal subjects (NS, P = 0.001, 0.001, and 0.008, ...

Research paper thumbnail of Post-apoptotic tumors are more palatable to dendritic cells and enhance their antigen cross-presentation activity

Vaccine, 2008

Critical issues for cytotoxic lymphocyte (CTL) cross-priming are (a) the maturation state of dend... more Critical issues for cytotoxic lymphocyte (CTL) cross-priming are (a) the maturation state of dendritic cells (DC), (b) the source of the tumor-associated antigens (TAA) and (c) the context in which they are delivered to DCs. Drug-induced apoptosis has recently been implicated in CTL cross-priming. However, since drug-treatment produces in vivo more tumor cells than the DC default apoptotic clearance program can cope with, they are expected to proceed to secondary necrosis and change their molecular pattern.

Research paper thumbnail of Influence of drug‐induced apoptotic death on processing and presentation of tumor antigens by dendritic cells

… journal of cancer, 2003

Here we have studied the effects of apoptotic cell death induced by chemotherapic agents on tumor... more Here we have studied the effects of apoptotic cell death induced by chemotherapic agents on tumor phagocytosis by dendritic cells (DC) and presentation of the relevant antigen to T lymphocytes. Annexin-V-FITC (Ann-V) and propidium iodide (PI) staining was used to assess early apoptotic (Ann-V ؉ /PI ؊ ) vs. late apoptotic/secondary necrotic (Ann-V ؉ /PI ؉ ) death after a 24 hr observation of untreated and drug-treated gastric carcinoma cells. After treatments, the HLA-A*0201 ؉ tumor cell line KATO III was exposed for 24 hr to allogeneic, HLA-related GM-CSF, IL-4-driven immature (i) DC. Tumorloaded iDC were tested for IL-12 release in an ELISA assay, incubated with the DC-maturating factor TNF-␣ and used as stimulators for autologous T lymphocytes.

Research paper thumbnail of A Late Quaternary canyon/channel system, northwest Gulf of Mexico continental slope

Abstract A late Quaternary submarine canyon and channel system, mapped on the north-west slope (l... more Abstract A late Quaternary submarine canyon and channel system, mapped on the north-west slope (long. 94–95 W) of the Gulf of Mexico with high-resolution (3.5 kHz) seismic reflection profiling and sidescan sonar, can be divided into two types of units:(1) leveed, ...

Research paper thumbnail of Prolactin is an autocrine growth factor for the Jurkat human T-leukemic cell line

Despite convincing evidence of cooperation between IL-2 and endogenous prolactin (PRL) during T c... more Despite convincing evidence of cooperation between IL-2 and endogenous prolactin (PRL) during T cell activation, the individual role of PRL as a T-cell lineage cytokine remains to be defined. We have examined the production and function of PRL on the Jurkat human T-leukemic cell line, which does not constitutively produce IL-2. The majority of Jurkat cells expressed PRL receptor (R) under standard culture conditions, whereas appearance of the alpha chain of the IL-2-R required PHA-PMA stimulation, as did IL-2 synthesis. Western blotting revealed a predominant band at 23.5 kDa and a weaker band at 25.5 kDa in both Jurkat cell lysates and human (h) pituitary PRL. Metabolic labeling of the cell lysates with 35S-methionine and immunoprecipitation with an antiserum against hPRL showed that both forms of PRL are actively synthesized by the Jurkat cell line. PRL released in the medium was biologically active in the rat Nb2 lymphoma mitogenic assay. Depletion of medium PRL with two polyclonal anti-hPRL antisera inhibited the growth of Jurkat cells in a dose-dependent manner, as evaluated by cell number and 3H-TdR uptake. Purified pituitary or recombinant hPRL at a wide range of concentrations had no significant effect on their growth, but reversed the blocking activity of the anti-hPRL antibody. Recombinant IL-2 had no effect on the antibody-induced growth inhibition. Taken as a whole, these results demonstrate that PRL can act as an autocrine T cell growth factor independently of IL-2 and are the first evidence of its involvement in human leukemic growth and possibly in leukemic transformation.

Research paper thumbnail of Endocrine, paracrine and autocrine actions of prolactin on immune cells

The immune response is regulated by locally released factors, collectively referred to as cytokin... more The immune response is regulated by locally released factors, collectively referred to as cytokines. Data on the human immune system have convincingly demonstrated that the hormone prolactin (PRL), in addition to exerting its endocrine control on the immune system, acts as a cytokine in that it is released within the immune system and regulates the lymphocyte response by paracrine and autocrine mechanisms. Both lymphocyte and pituitary PRLs are under the control of immune factors. Synthesis of human PRL by lymphocytes is induced by T-cell stimuli, while increased release of PRL by the pituitary, observed in vivo after immune challenge, may be mediated by cytokines produced by monocyte-macrophages. Since hyperprolactinemia and hypoprolactinemia are both immunosuppressive, physiological levels of circulating PRL must be necessary to maintain basal immunocompetence. The effects of Cyclosporin (CsA) on IL-2 and PRL gene activation and the analysis of the intracellular signaling events downstream IL-2 and PRL receptors suggest coordinate actions of these two cytokines during T cell activation.

Research paper thumbnail of Platelet-Activating Factor-Stimulated In Vitro Erythropoiesis Bone Marrow Stroma-Derived Prolactin Is Involved in Basal and

Research paper thumbnail of In vitro maturation of dendritic cells from blood progenitors

Methods in molecular biology (Clifton, N.J.), 2003

Research paper thumbnail of Effect of two aliphatic aldehydes, methylglyoxal and 4-hydroxypentenal, on the growth of Yoshida ascites hepatoma AH-130

The influence of a ketoaldehyde, methylglyoxal (MG), and a hydroxyalkenal, 4-hydroxypentenal (HPE... more The influence of a ketoaldehyde, methylglyoxal (MG), and a hydroxyalkenal, 4-hydroxypentenal (HPE), on the growth of a highly-deviated turnout has been investigated. MG and HPE, administered intraperitoneally, strongly depressed in rats the proliferative activity of the Yoshida ascites hepatoma AH-130, reducing its mitotic and labelling indices as well as the proportion of cycling cells (growth fraction). Monitoring the effects on the cell cycle by the labelled mitoses method showed that the percentage of labelled mitoses was markedly lowered after either aldehyde, which is indicative for a blocking effect in the S phase. In addition, the mean cell cycle time was slightly prolonged by MG, probably due to accumulation of cells in G1, whereas HPE delayed the first mitotic peak and increased the mean DNA synthetic period without modifying the overall cycle time. The effects of HPE on the cell cycle were prevented by pretreatment with polyamines. Bvpw~cd do~c~ of MG ~ignlflcantly increased the fraction of tumour-bearlng rats surviving at 90 days ('indefinite' survivors) as well as the survival time of those which succumbed, implying that the carcinostatic effect of MG persisted over several cell cycles. By contrast, HPE did not significantly modify the survival of AH-130-bearing rats, suggesting that its influence on tumour growth was rapidly reversible.

Research paper thumbnail of Immunogenicity of 56° C and UVC‐treated prostate cancer is associated with release of HSP70 and HMGB1 from necrotic cells

The Prostate, 2009

BACKGROUND Prostate hyperthermia and photodynamic therapy can be delivered by a variety of proced... more BACKGROUND Prostate hyperthermia and photodynamic therapy can be delivered by a variety of procedures which result in a wide range of temperatures and light energy and cause different kinds of cell death. METHODS We have addressed the immunogenic effect ...

Research paper thumbnail of Increased expression of HSP70 by colon cancer cells is not always associated with access to the dendritic cell cross-presentation pathway

Cellular & Molecular …, 2007

Abstract: Dendritic cells (DCs) are highly specialized antigen-presenting cells endowed with the ... more Abstract: Dendritic cells (DCs) are highly specialized antigen-presenting cells endowed with the unique ability to not only present exogenous antigens upon exposure to MHC II, but also to cross-present these upon exposure to MHC I. This property was exploited to generate ...

Research paper thumbnail of Prostatectomy restores the maturation competence of blood dendritic cell precursors and reverses the abnormal expansion of regulatory T lymphocytes

The …, 2010

Despite loss of the monocytes marker CD14, cytokine-matured DCs of tumor bearing patients express... more Despite loss of the monocytes marker CD14, cytokine-matured DCs of tumor bearing patients expressed lower levels of the costimulatory molecule CD80 and of the maturation markers CD83 and CCR7 compared to mDC of normal subjects (NS, P = 0.001, 0.001, and 0.008, ...

Research paper thumbnail of Post-apoptotic tumors are more palatable to dendritic cells and enhance their antigen cross-presentation activity

Vaccine, 2008

Critical issues for cytotoxic lymphocyte (CTL) cross-priming are (a) the maturation state of dend... more Critical issues for cytotoxic lymphocyte (CTL) cross-priming are (a) the maturation state of dendritic cells (DC), (b) the source of the tumor-associated antigens (TAA) and (c) the context in which they are delivered to DCs. Drug-induced apoptosis has recently been implicated in CTL cross-priming. However, since drug-treatment produces in vivo more tumor cells than the DC default apoptotic clearance program can cope with, they are expected to proceed to secondary necrosis and change their molecular pattern.