Linhong Deng - Academia.edu (original) (raw)

Papers by Linhong Deng

Journal of Advances in Biomedical Engineering and Technology, 2016

Chinese Science Bulletin, May 15, 2013

Science Translational Medicine, Feb 7, 2018

International Journal of Biological Sciences, 2022

Frontiers in Molecular Biosciences, 2021

Airway smooth muscle cells (ASMCs) exist in a form of helical winding bundles within the bronchia... more Airway smooth muscle cells (ASMCs) exist in a form of helical winding bundles within the bronchial airway wall. Such tubular tissue provides cells with considerable curvature as a physical constraint, which is widely thought as an important determinant of cell behaviors. However, this process is difficult to mimic in the conventional planar cell culture system. Here, we report a method to develop chips with cell-scale tubular (concave and convex) surfaces from fused deposition modeling 3D printing to explore how ASMCs adapt to the cylindrical curvature for morphogenesis and function. Results showed that ASMCs self-organized into two distinctively different patterns of orientation on the concave and convex surfaces, eventually aligning either invariably perpendicular to the cylinder axis on the concave surface or curvature-dependently angled on the convex surface. Such oriented alignments of the ASMCs were maintained even when the cells were in dynamic movement during migration and s...

Experimental physiology, 2018

What is the central question of this study? What is the effect of catenin alpha-like 1 (CTNNAL1),... more What is the central question of this study? What is the effect of catenin alpha-like 1 (CTNNAL1), an asthma-related epithelial adhesion molecule that plays a vital role in airway epithelial wound repair, on airway epithelial-mesenchymal transition? What is the main finding and its importance? CTNNAL1 inhibits ozone-induced airway epithelial-mesenchymal transition features, mediated by repressing the expression of Twist1 mRNA and reducing TGF-β1 levels. These findings contribute to our understanding of the pathology of airway EMT and may indicate a possible therapeutic target for airway remodelling in bronchial asthma. Epithelial-mesenchymal transition (EMT), a crucial event occurring during epithelial and mesenchymal repair, was reported to be a possible mechanism for airway remodelling. Our previous work showed that the expression of catenin alpha-like 1 (CTNNAL1) was down-regulated in the bronchial epithelial cells of asthmatic models and played a vital role in airway epithelial w...

Experimental and therapeutic medicine, 2018

Ozone (O) as a major air pollutant is widely recognized for causing pathological changes of the a... more Ozone (O) as a major air pollutant is widely recognized for causing pathological changes of the airway system. However, it is not clear whether O exposure of bronchial epithelial cells (BECs) influences the proliferation and collagen synthesis of submucosal fibroblasts and contributes to the pathogenesis of airway remodeling in diseases, including asthma. In the present study, a co-culture method was applied to culture human lung fibroblasts (HLFs) with human bronchial epithelial cells (HBECs) that were pre-stimulated with O. Following co-culture for up to 24 h, the proliferation of HLFs was measured using MTT colorimetry. Furthermore, the collagen synthesis capacity of HLFs was determined by the level of hydroxyproline. In addition, the protein expression levels of cytokines, including transforming growth factor (TGF)-β1, tumor necrosis factor (TNF)-α and prostaglandin E2 (PGE2) were assessed. Results indicated that the proliferation of HLFs co-cultured with HBECs was significantly...

The Journal of allergy and clinical immunology, Jan 25, 2017

Allergic inflammation has long been implicated in asthmatic hyperresponsiveness of airway smooth ... more Allergic inflammation has long been implicated in asthmatic hyperresponsiveness of airway smooth muscle (ASM), but its mechanism underlying remains incompletely understood. Serving as GPCR agonists, several inflammatory mediators can induce membrane depolarization, contract ASM and augment cholinergic contractile response. We hypothesized that the signal cascade integrating on membrane depolarization by the mediators might involve asthmatic hyperresponsiveness. We sought to investigate the signalling transduction of inflammatory mediators in ASM contraction and assess its contribution in the genesis of hyperresponsiveness. We assessed the capacity of inflammatory mediators to induce depolarization currents by electrophysiological analysis. We analysed the phenotypes of TMEM16A knockout mice, applied pharmacological reagents and measured the Ca(2+) signal during ASM contraction. To study the role of the depolarization signalling in asthmatic hyperresponsiveness, we measured the syner...

Scientific reports, Jul 26, 2016

A disintegrin and metalloproteinase 8 (ADAM8) has been identified as a signature gene associated ... more A disintegrin and metalloproteinase 8 (ADAM8) has been identified as a signature gene associated with moderate and severe asthma. Studies in mice have demonstrated that the severity of asthma can be reduced by either transgenic knock-out or by antibodies blocking ADAM8 function, highlighting ADAM8 as potential drug target for asthma therapy. Here, we examined the therapeutic effect of an ADAM8 inhibitor peptide (BK-1361) that specifically blocks cellular ADAM8 activity in ovalbumin-sensitized and challenged Balb/c mice. We found that BK-1361 (25 μg/g body weight) attenuated airway responsiveness to methacholine stimulation by up to 42%, concomitantly reduced tissue remodeling by 50%, and decreased inflammatory cells (e.g. eosinophils down by 54%)/inflammatory factors (e.g. sCD23 down by 50%)/TH2 cytokines (e.g. IL-5 down by 70%)/ADAM8-positive eosinophils (down by 60%) in the lung. We further verified that BK-1361 specifically targets ADAM8 in vivo as the peptide caused significantl...

Physiological Reviews, 2009

The mechanical properties of lung tissue are important determinants of lung physiological functio... more The mechanical properties of lung tissue are important determinants of lung physiological functions. The connective tissue is composed mainly of cells and extracellular matrix, where collagen and elastic fibers are the main determinants of lung tissue mechanical properties. These fibers have essentially different elastic properties, form a continuous network along the lungs, and are responsible for passive expiration. In the last decade, many studies analyzed the relationship between tissue composition, microstructure, and macrophysiology, showing that the lung physiological behavior reflects both the mechanical properties of tissue individual components and its complex structural organization. Different lung pathologies such as acute respiratory distress syndrome, fibrosis, inflammation, and emphysema can affect the extracellular matrix. This review focuses on the mechanical properties of lung tissue and how the stress-bearing elements of lung parenchyma can influence its behavior.

Hepatitis Monthly, 2015

The molecular mechanisms of tumor suppressor gene DLEC1 are largely unknown. In this study, we es... more The molecular mechanisms of tumor suppressor gene DLEC1 are largely unknown. In this study, we established DLEC1 over-expression stable clones to study the cellular function of DLEC1 in the colorectal cancer cell line, HCT116. Stable clones with DLEC1 over-expression were first established by the transfection of DLEC1 expression construct pcDNA31DLEC1 in HCT116. On G418 selection, positive stable clones were screened for DLEC1 expression level by conventional reverse transcription-polymerase chain reaction (RT-PCR), and verified by real-time RT-PCR and Western blotting. Subsequently, these stable clones were subjected to colony formation and cell cycle analyses and identification of factors involved in G1 arrest. Lastly, three stable clones, DLEC1-7 (highest DLEC1 expression), DLEC1-3 (lowest expression) and pcDNA31 vector control, were employed to analyze cell proliferation and cell cycle after AP-2α2 knockdown by siRNAs. The DLEC1 over-expression was found to reduce the number of colonies in colony formation and to induce G1 arrest in seven clones, and apoptosis in one clone in the cell cycle analysis. Furthermore, regardless of the different cell cycle defects in all eight stable clones, the expression level of transcriptional factor AP-2α2 was found to be elevated. More interestingly, we found that when AP-2α2 was knocked down, DLEC1 over-expression neither suppressed cancer cell growth nor induced G1 arrest, yet, instead promoted cell growth and decreased cells in the G1 fraction. This promotion of cell proliferation and release of G1 cells also seemed to be proportional to DLEC1 expression levels in DLEC1 stable clones. DLEC1 suppresses tumor cell growth the presence of AP-2α2 and stimulates cell proliferation in the down-regulation of AP-2α2 in DLEC1 over-expression stable clones of HTC116.

Applied Clay Science, 2014

A clay polymer nanocomposite (CPN) based on aspirin-loaded palygorskite (Pal) modified polypyrrol... more A clay polymer nanocomposite (CPN) based on aspirin-loaded palygorskite (Pal) modified polypyrrole (PPy) was prepared by in situ electropolymerization of pyrrole monomer in the presence of Pal as the modifier and aspirin as the drug source. This drug-loading approach was simple and convenient, since colloid templates such as polystyrene microspheres used in conventional drug-loading system were not needed. The resulting CPN was characterized by TEM, XRD, cyclic voltammetry (CV), chronocoulometry, electrochemical impedance spectroscopy (EIS) and FTIR. The CPN was used as a new platform for aspirin delivery, which could significantly enhance aspirin loading capacity of the system and control aspirin release by external electrical stimulus. The results indicated that the proposed novel drug-delivery system might be promising as an implantable device where drug release could be electrically tuned according to the patient's requirement.

Applied Clay Science, 2013

Palygorskite (Pal) may be a promising material for enzyme immobilization due to its large surface... more Palygorskite (Pal) may be a promising material for enzyme immobilization due to its large surface, high biocompatibility and stability. This attractive material combined with a conducting polymer, poly(ophenylenediamine), was exploited as a platform for the immobilization of glucose oxidase (GOD) using glutaraldehyde as crosslinker, and thus a novel glucose biosensor was obtained. The results of electrochemical impedance spectroscopy (EIS) and SEM indicated the successful entrapment of GOD in the clay polymer nanocomposite (CPN) film. Amperometric detection of glucose was performed by holding the potential at the CPN electrode at 0.6 V for the oxidation of H 2 O 2 generated in the enzymatic reaction. The apparent Michaelis-Menten constant (K M app ) was calculated to be 5.25 mM, which is close to that of the free enzyme. The proposed biosensor exhibited a wide linear range, a low detection limit, a good reproducibility and accepted stability in the determination of glucose, providing a biocompatible platform for glucose biosensing.

Nanoscale research letters, 2011

Protein-protein interactions play crucial roles in numerous biological processes. However, it is ... more Protein-protein interactions play crucial roles in numerous biological processes. However, it is still challenging to evaluate the protein-protein interactions, such as antigen and antibody, in the presence of drug molecules in physiological liquid. In this study, the interaction between bovine serum albumin (BSA) and rabbit anti-BSA was investigated using atomic force microscopy (AFM) in the presence of various antimicrobial drugs (sulphathiazole sodium, tylosin and levofloxacin) under physiological condition. The results show that increasing the concentration of tylosin decreased the single-molecule-specific force between BSA and rabbit anti-BSA. As for sulphathiazole sodium, it dramatically decreased the specific force at a certain critical concentration, but increased the nonspecific force as its concentration increasing. In addition, the presence of levofloxacin did not greatly influence either the specific or nonspecific force. Collectively, these results suggest that these th...

Journal of Biological Physics, 2013

Depletion effects are well known to lead to phase separation in microsystems consisting of large ... more Depletion effects are well known to lead to phase separation in microsystems consisting of large and small particles with short-range repulsive interactions that act over macromolecular length scales. The equilibrium mechanics between an enveloped colloidal particle and a biomembrane caused by entropy is investigated by using a continuum model. We show that the favorable contact energy stems from entropy, which is sufficient to drive engulfment of the colloidal particle, and deformation of the biomembrane determines the resistance to the engulfment of the colloidal particle. The engulfment process depends on the ratio of the radii of the larger particle and smaller particles and the bending rigidity. The results show insights into the effects of depletion on biomembrane budding and nanoparticle transportation by a vesicle.

Biorheology

The electrical and dielectrical properties of blood are, in many ways, similar to the rheological... more The electrical and dielectrical properties of blood are, in many ways, similar to the rheological ones. A novel measurement system has been constructed to realize a concurrent measurement of both electrical and rheological properties of blood. The system is based on a Contraves LS30 rheometer. Ring electrodes of brass base with gold-coated surface were incorporated into the measuring cup of Teflon. A rotech three way slip ring-brush unit connects a Wayne Kerr B642 bridge with the electrodes on the rotating cup to record the variation of blood electrical conductance and capacitance while the LS30 simultaneously takes the measurement of shearinduced torque on the blood through blood. 0.9% saline, pig's blood and human blood and blood cell suspensions in different medium were tested on this system. It was found that whole blood conductance has an outstanding response upon shearing than blood cells suspension in saline. The elevation of blood conductance due to shear can be up to 15%. This corresponds to the change of cells' shape factor f r =(1) (G o ). Blood has an exponential recovery after shear as an indication of aggregation or rouleau formation while a distinctively different recovery process was found in RBC suspensions in saline. It is concluded that by using the above system, the effect of influential factors in blood rheology can well be detected by the electrical properties of it, which could bring another dimension in hemorheology studies.