Livio Giuliani - Academia.edu (original) (raw)

Papers by Livio Giuliani

Biomagnetic research and …, 2004

... There is almost no remnant magnetization at 300 K but at 5 K, hysteresis loop was observed (F... more ... There is almost no remnant magnetization at 300 K but at 5 K, hysteresis loop was observed (Fig 4). This ... Adv Mater 1998, 10:827-837. 3. Zrinyi M, Barsi L, Buki A: Ferrogel: a new magneto-controlled elastic medium. Polym Gels Netw 1997, 5:415-427. ...

International Journal of Vaccine Theory, Practice, and Research

The engineered spike protein of SARS-COV-2, and the corresponding infectious disease COVID-19 att... more The engineered spike protein of SARS-COV-2, and the corresponding infectious disease COVID-19 attributed to it, hold in their grip a large portion of humanity. The global race for a counter strategy quickly turned into a search for a vaccine as the preferred means to contain the virus. An unusually rapid development of different and completely new classes of experimental therapies that would widely be referred to as “vaccines” raised questions about safety, especially with regard to emergency use approval (EUA) being granted with unprecedented urgency and hardly any critical scrutiny. At present, independent researchers, even some former proponents and insiders, of the currently ongoing global experiment represented as a “vaccination” campaign point primarily to the lack of public safety studies based on empirical datasets that should be obtainable for the tens of millions, even hundreds of millions, of doses of mRNA and DNA vector therapeutics being distributed as “vaccines”. Studi...

Electricity and Magnetism in Biology and Medicine, 1999

Possibility of negative effects on health from exposure to radio frequency (RF) or extremely low ... more Possibility of negative effects on health from exposure to radio frequency (RF) or extremely low frequency (ELF) fields has stimulated the increase in recent years of publications on the biological effects of electric and magnetic fields l, 2, 3. Particularly debated is the question of the epidemiological evidence of adverse effects of ELF fields generated by 50–60 Hz high voltage power transmission lines, video display terminals, electric blankets and other home appliances. Reported ranges of exposure are: magnetic flux density (B): 0.1–1000 μT; and electric field intensity: 0.01-100 V/cm (in air). This value of external field is reduced by about seven orders of magnitude to the μV/cm range when air-capacity coupled to conductive organisms or tissues. An electric field may also be set up in tissues and cells by a time-varying magnetic field according to Faraday’s law. In humans, the peak values of this magnetically induced electrical field, also fall in the μV/cm range for ambient values of μT. 4, 5 A strong objection to the acceptance of reported weak field effects is that their intensity is many orders of magnitude below the noise threshold 6 so that selective, cooperative or amplifying mechanisms, must be postulated. The minimum value of applied field to which a cell would respond without any such mechanism has been calculated to be about 1 mV/cm for a large elongated cell and 20–40 mV/cm for a spherical cell 6, 7. Much higher magnetic flux densities, pulsed or sinusoidal, in the 1–10 mT range (induced electric fields: 1-10 mV/cm) are produced by some industrial processes and by clinical (gradient fields in NMR imaging) and therapeutic procedures (healing of wounds and bone fractures). In general, a physiological framework for EMF effects has still to be established. This may be attempted by studying the mechanisms by which EMFs interact with cells under controlled laboratory conditions and by correlating in vivo evidence with in vitro data. Reviews of related studies on tissues and cells may be found in literature. 8 In their pioneering work Bawin and Adey in the middle 1970s 9 reported a 15% decrease of Ca2+ efflux in chick and cat cerebral tissue after a 20 min exposure to an electric field of 10 V/m in air (in the ambient range).

Vibrational Spectroscopy, 2010

Ultrasonics, 2009

The structural effect induced by therapeutic ultrasound on proteins in aqueous solution has been ... more The structural effect induced by therapeutic ultrasound on proteins in aqueous solution has been investigated with FTIR spectroscopy, UV-VIS spectroscopy, circular dichroism and light scattering. Six proteins (cytochrome, lysozyme, myoglobin, bovine serum albumin, trypsinogen, and a-chymotrypsinogen A) with different molecular weight and secondary structure have been studied. The experiment has been performed using an ultrasound source at resonant frequency of 1 MHz and sonication times of 10, 20, 30, 40, 50, and 60 min. A different behaviour of proteins under sonication depends on the dominant secondary structure type (a-helix or b-sheets) and on the grade of the ordered structure. The results suggest that the free radicals, produced by water sonolysis, have an important role in the changes of structural order.

Radiation

In order to test the theory of Brizhik et al. about the dynamic allocation of acupuncture meridia... more In order to test the theory of Brizhik et al. about the dynamic allocation of acupuncture meridians on human body and their role as hydric paths for solitons, we tested the effect of both acupuncture and exposure to extremely low frequencies (ELF) tuned with suitable ion cyclotron frequencies. The similarity of the effects, inducing variations of body impedance measured in well-known acupuncture points, up to the interchangeability and the synergy of the two treatments, the mechanic and the electromagnetic ones, turns to be evidence of the validity of the theory. Resistance and reactance variations have been detected in a group of 31 healthy volunteers before and after stimulation with a standard sequence of cyclotron frequencies, emitted from an innovative electromagnetic therapy (EMT) device. These variations were then compared with the variations produced by the well-known percutaneous stimuli of mechanical and piezoelectric nature, and, in particular in this work, acupuncture. O...

Applied Sciences, 2021

The global rise of antimicrobial resistance (AMR) constitutes a future health threat and dictates... more The global rise of antimicrobial resistance (AMR) constitutes a future health threat and dictates a need to explore alternative and non-chemical approaches. The aim of this study was to explore the use of weak resonant electromagnetic fields as a method to disrupt biofilm formation of a pathogenic bacterium in cystic fibrosis patients. We developed a bioresonance laboratory setup able to distinguish between changes in planktonic growth and changes in biofilm formation and showed that certain resonant frequencies were able to affect biofilm formation without affecting planktonic growth. In addition, we show that the ambient day-to-day magnetic field affects biofilm formation in a non-consistent manner. Overall, we conclude that our assay is suitable for studying the potential of resonant magnetic fields as a treatment and prevention strategy to prevent biofilm infections, and that certain resonant frequencies may be used as future medical applications to combat antimicrobial resistance.

Aerosol and Air Quality Research, 2021

Journal of Vaccines and Vaccination, 2017

Influenza is a viral disease; it spreads during the winter season in the form of epidemics or eve... more Influenza is a viral disease; it spreads during the winter season in the form of epidemics or even pandemics. Its clinical and social importance is highly amplified by International and National (mainly in Italy) Health Authorities in order to promote and justify the vaccination campaigns. This aim is achieved by providing distorted or fake information to the public. These distortions mainly concern 4 aspects: 1) the real diffusion of influenza viruses; 2) the death rate relative to this disease; 3) vaccine effectiveness; 4) the presence of new viruses in new vaccines. Each of these points will be analysed. Real data, reviews of the literature and arguments will be shown for comparison. The conclusion is that: 1) the spread of the disease is about 10 times lower to the declared one; 2) mortality is very modest, questionable and anyway far less than what was declared; 3) vaccine effectiveness has always been proved as being far low than what had previously been declared, and it has o...

The Mechanical Vibration: Therapeutic Effects and Applications, 2017

$Research paper thumbnail of Weak-field H3O+ion cyclotron resonance alters water refractive index$

Electromagnetic Biology and Medicine, 2016

ABSTRACT Heretofore only observed in living systems, we report that weak-field ion cyclotron reso... more ABSTRACT Heretofore only observed in living systems, we report that weak-field ion cyclotron resonance (ICR) also occurs in inanimate matter. Weak magnetic field (50 nT) hydronium ICR at the field combination (7.84 Hz, 7.5 µT) markedly changes water structure, as evidenced by finding an altered index of refraction exactly at this combined field. This observation utilizes a novel technique which measures the scattering of a He–Ne laser beam as the sample is exposed to a ramped magnetic field frequency. In addition to the hydronium resonance, we find evidence of ICR coupling to a more massive structure, possibly a tetrahedral combination of three waters and a single hydronium ion. To check our observations, we extended this technique to D2O, successfully predicting the specific ICR charge-to-mass ratio for D3O+ that alters the index of refraction.

Electromagnetic Biology and Medicine, 2014

Abstract There is an ongoing question regarding the structure forming capabilities of water at am... more Abstract There is an ongoing question regarding the structure forming capabilities of water at ambient temperatures. To probe for different structures, we studied effects in pure water following magnetic field exposures corresponding to the ion cyclotron resonance of H3O(+). Included were measurements of conductivity and pH. We find that under ion cyclotron resonance (ICR) stimulation, water undergoes a transition to a form that is hydroxonium-like, with the subsequent emission of a transient 48.5 Hz magnetic signal, in the absence of any other measurable field. Our results indicate that hydronium resonance stimulation alters the structure of water, enhancing the concentration of EZ-water. These results are not only consistent with Del Giudice's model of electromagnetically coherent domains, but they can also be interpreted to show that these domains exist in quantized spin states.

International journal of biomedical science : IJBS, 2009

Investigations into the ion cyclotronic resonance (ICR) in living matter confront the so called Z... more Investigations into the ion cyclotronic resonance (ICR) in living matter confront the so called Zhadin effect (12), whose explanation is not fully achieved. Several attempts have been done to explain this phenomenon, the most interesting of which is based on Quantum Electrodynamics (18): the molecules of water, the ions and the biomolecules form extended mesoscopic regions, called Coherence Domains (CD), where they oscillate in unison between two selected levels of their spectra in tune with a self-produced coherent E.M. field having a well defined frequency, dynamically trapped within the CD. Moreover, it is possible, to induce, by an external applied field (either hydrodynamical or EM) or also by a chemical stimulation, coherent excitations of CD's that give rise to electric currents circulating without friction within the CD's: as a consequence magnetic fields are produced. A resonating magnetic field thus is able to extract the ions from the orbit and push them in the fl...

Session 2P1b Biophoton, …, 2008

ABSTRACT Studies for the identification of suitable stem cells culture and differentiating con-di... more ABSTRACT Studies for the identification of suitable stem cells culture and differentiating con-ditions that are devoid of xenogenic growth supplements is actually considered an important issue for the clinical applicability of cell therapy for heart failure and bone remodeling, growth and repair. About the first one we have recently demonstrated the possibility to obtain Cardiac Stem Cells (CSCs), from human endomyocardial biopsy specimens. CSCs self-assemble into multi-cellular clusters known as cardiospheres (CSps) that engraft and partially regenerate infarcted myocardium. CSps and Cardiospheres-derived-Cells CDCs were exposed for five days in an incubator inside a solenoid system with temperature and humidity and CO 2 regulated. This exposure system were placed in an amagnetic shielded room in the simultaneous presence of a static MF and a low-alternating-frequency-MF, close to the cyclotron frequency corresponding to the charge/mass ratio of Ca ++ ion. In this exposure conditions CSps and CDCs modulate their differentiation turning on cardiogenesis and turning off vasculogenesis. Cardiac markers such as Troponin I (TnI) or Myosin Heavy Chain (MHC) were up-regulated, conversely angiogenic markers such as Vascu-lar Endothelial Growth Factor (VEGF) or Kinase Domain Receptor (KDR) were down-rugulated as evidenced by immunocytochemistry. The improvement in the cardiogenic differentiation was confirmed by Real-Time PCR and Western Blotting. Interestingly, an increase in the prolifer-ation (particularly of the CDCs) was observed and evidenced by Brd-U incorporation (ELISA) and cell counting kit-8 (WST-8) analysis. Exposure to Calcium ion ICR can modulates the cardiogenic versus angiogenic differentiation process of ex vivo expanded CSCs.This may pave the way for novel approaches in tissue engi-neering and cell therapy. Concerning bone remodelling our study shows that exposure of human MSC to ELF-MF enhanced expression of osteoblast marker differentiation such as Alkaline phosphatase, (AP), Osteocalcin (OCL), and osteopontin (OPN), analyzed by real-time quantitative PCR, without affecting cell proliferation. As expected, while the markers differentiation factors where up regulated, elec-tromagnetic field down regulate osteoprotegerin (OPG) gene expression, a critical regulator of postnatal skeletal development and homeostasis in humans as well as mice. The exposure of hMSC for 5 days to the field resulted in a change in shape and in plasma membrane morphology and this modification were also accompanied by a rearrangement in actin filaments, as showed by confocal miscroscopy analysis after cells labelling with FITC-phalloidin.

The use of registered names, trademarks, etc. in this publication does not imply, even in the abs... more The use of registered names, trademarks, etc. in this publication does not imply, even in the absence of a specific statement, that such names are expempt from the relevant protective laws and regulations and therefore free for general use. Product liability: the publishers cannot guarantee the accuracy of any information about dosage and application contained in this book. In every individual case the user must check such information by consulting the relevant literature.

The fundamental Physical mechanisms of the resonant action of an extremely weak (40 nanoT) altern... more The fundamental Physical mechanisms of the resonant action of an extremely weak (40 nanoT) alternating magnetic field at the cyclotron frequency combined with a weak (40 mcT) static magnetic field, on living systems are analyzed in the present article. The experimental effects of such sort of magnetic fields were described in different articles: the very narrow resonant peaks in electrical conductivity of the aqueous solutions in the in vitro experiments and the Biomedical in vivo effects on living animals of magnetic fields with frequencies tuned to some amino acids. The existing experimental in vitro data had a good repeatability in different laboratories and countries. Unfortunately, for free ions such sort of effects are absolutely impossible because the dimensions of an ion rotation radius should be measured by meters at room temperature and at very low static magnetic fields used in all the above experiments. Even for bound ions these effects should be also absolutely impossib...

Journal of Physics: Conference Series, 2011

A high throughput screening system of coils for ELF magnetic fields experiments: proof of concept... more A high throughput screening system of coils for ELF magnetic fields experiments: proof of concept on the proliferation of cancer cell lines Leonardo Makinistian et al-Rate limiting factors for DNA transduction induced by weak electromagnetic field B.

Basic & Clinical Pharmacology & Toxicology

Epidemiological studies have suggested that human exposure to extremely low-frequency electromagn... more Epidemiological studies have suggested that human exposure to extremely low-frequency electromagnetic fields from the electric power and to mobile phone radiofrequency electromagnetic fields induce an increased risk of developing malignant tumours. However, no adequate laboratory data, in particular long-term carcinogenicity bioassays to support the epidemiological evidence, have yet been available.

American Journal of Industrial Medicine, 2016

Experimental rodent bioassays performed up to now have failed to provide conclusive confirmation ... more Experimental rodent bioassays performed up to now have failed to provide conclusive confirmation of the carcinogenicity of extremely low frequency magnetic fields (ELFMF). To evaluate the potential synergistic carcinogenic effects of concurrent exposure to ELFMF and formaldehyde in four groups of male and female Sprague-Dawley rats. One group was exposed from prenatal life until natural death to S-50 Hz MF and to formaldehyde in drinking water from 6 weeks of age for 104 weeks, two groups were treated only with formaldehyde or only with MF and one group served as untreated control. Compared to untreated controls, exposure to MF and formaldehyde causes in males a statistically significant increased incidence of malignant tumors (P ≤ 0.01), thyroid C-cell carcinomas (P ≤ 0.01), and hemolymphoreticular neoplasias (P ≤ 0.05). No statistically significant differences were observed among female groups. Life-span exposure to MF and formaldehyde induces statistically significant carcinogenic effects in male rats. Am. J. Ind. Med. 59:509-521, 2016. © 2016 Wiley Periodicals, Inc.