E. Loew - Academia.edu (original) (raw)
Papers by E. Loew
Journal of the American Veterinary Medical Association, 1985
ABSTRACT NCAR's Electra Doppler radar (ELDORA) with a dual-beam slotted waveguide array u... more ABSTRACT NCAR's Electra Doppler radar (ELDORA) with a dual-beam slotted waveguide array using dual-transmitter, dual-beam, rapid scan and step-chirped waveform significantly improved the spatial scale to 300m (Hildebrand et al. 1996). However, ELDORA X-band radar's penetration into precipitation is limited by attenuation and is not designed to collect polarimetric measurements to remotely estimate microphysics. ELDORA has been placed on dormancy because its airborne platform (P3 587) was retired in January 2013. The US research community has strongly voiced the need to continue measurement capability similar to the ELDORA. A critical weather research area is quantitative precipitation estimation/forecasting (QPE/QPF). In recent years, hurricane intensity change involving eye-eyewall interactions has drawn research attention (Montgomery et al., 2006; Bell and Montgomery, 2006). In the case of convective precipitation, two issues, namely, (1) when and where convection will be initiated, and (2) determining the organization and structure of ensuing convection, are key for QPF. Therefore collocated measurements of 3-D winds and precipitation microphysics are required for achieving significant skills in QPF and QPE. Multiple radars in dual-Doppler configuration with polarization capability estimate dynamical and microphysical characteristics of clouds and precipitation are mostly available over land. However, storms over complex terrain, the ocean and in forest regions are not observable by ground-based radars (Bluestein and Wakimoto, 2003). NCAR/EOL is investigating potential configurations for the next generation airborne radar that is capable of retrieving dynamic and microphysical characteristics of clouds and precipitation. ELDORA's slotted waveguide array radar is not compatible for dual-polarization measurements. Therefore, the new design has to address both dual-polarization capability and platform requirements to replace the ELDORA system. NCAR maintains a C-130 aircraft in its fleet for airborne atmospheric measurements, including dropsonde, and in situ sampling and remote sensing of clouds, chemistry and aerosols. Therefore, the addition of a precipitation radar to the NSF/NCAR C-130 platform will produce transformational change in its mission. This new design can be cloned for C-130s operated by a number of agencies, including NOAA and the Air Force hurricane reconnaissance fleet. This paper presents a possible configuration of a novel, airborne phased array radar (APAR) to be installed on the NSF/NCAR C-130 aircraft with improved spatial resolution and polarimetric capability to meet or exceed that of ELDORA. The preliminary design, an update of the APAR project, and a future plan will be presented. References: Bell, M. M. , M. T. Montgomery, 2008: Observed Structure, Evolution, and Potential Intensity of Category 5 Hurricane Isabel (2003) from 12 to 14 September. Monthly Weather Review, Vol. 136, Issue 6, pp. 2023-2046. Hildebrand, P. H., W.-C. Lee, C. A. Walther, C. Frush, M. Randall, E. Loew, R. Neitzel, R. Parsons, J. Testud, F. Baudin, and A. LeCornec, 1996: The ELDORA/ASTRAIA airborne Doppler weather radar: High resolution observations from TOGA COARE. Bull. Amer. Metoro. Soc., 77, 213-232 Howard B. Bluestein, Roger M. Wakimoto, 2003: Mobile Radar Observations of Severe Convective Storms re Convective Storms. Meteorological Monographs, Vol. 30, Issue 52, pp. 105-105. Montgomery, M. T., M. M. Bell, S. D. Aberson, M. L. Black, 2006: Hurricane Isabel (2003): New Insights into the Physics of Intense Storms. Part I: Mean Vortex Structure and Maximum Intensity Estimates. Bull. of the American Meteorl. Soc., Vol. 87, Issue 10, pp. 1335-1347.
Visual Neuroscience, 1996
Retinas of the nocturnal geckos, Hemidactylus turcicus, Hemidactylus garnotii, and Teratoscincus ... more Retinas of the nocturnal geckos, Hemidactylus turcicus, Hemidactylus garnotii, and Teratoscincus scincus, were studied with microspectrophotometry and immunocytochemistry against various visual pigment epitopes to reveal UV-sensitive photoreceptors. From 6-20% of the thinner members of type C double photoreceptors, earlier believed to be blue-sensitive, were found to contain a UV-absorbing visual pigment with lambda max at 363-366 nm. The pigment had bleaching and dichroic properties typical of other photoreceptor cell types of the retina. Presumptive UV-sensitive cells in retinal sections were "negatively" labeled as they did not react with either the cone-specific monoclonal antibody COS-1 or with the anti-rhodopsin polyclonal serum AO, which together labeled all of the remaining photoreceptor types (green-sensitive A singles, B doubles, and thicker members of C doubles, as well as the blue-sensitive majority of thinner members of C doubles). UV cells were moderately stained with the mAb K42-41 produced against the 5-6 loop of bovine rhodopsin, which also moderately labeled blue-sensitive cells. mAb OS-2 strongly stained all outer segments, including the UV-sensitive ones. Similarities between gecko UV visual pigments, and UV visual pigments of other vertebrates, as well as possible functional significance of these cells are discussed.
Visual Neuroscience, 2001
Absorbance spectra of cone outer segments and oil droplets were recorded microspectrophotometrica... more Absorbance spectra of cone outer segments and oil droplets were recorded microspectrophotometrically in the retina of the red-eared turtle, Trachemys scripta elegans. There are four cone visual pigments, with lambda(max) = 617 nm (red sensitive), 515 nm (green sensitive), 458 nm (blue sensitive), and 372 nm (UV-sensitive). The red-sensitive pigment resides in single cones with red or orange oil droplets, and in both members of double cones. The principal member of the double cone contains an orange oil droplet, and the accessory member is droplet free. The green-sensitive pigment is situated in single cones with orange/dark yellow droplets. The blue-sensitive pigment is combined with the UV-absorbing oil droplet in single cones. The UV-sensitive pigment resides in single cones with clear oil droplets that exhibited virtually no absorbance down to 325 nm. Thus, seven types of cones can be identified based on their morphology, oil droplet color, and the visual pigment absorbance. At the moment, this is the most complex cone system described for vertebrates.
Vision Research, 1992
As part of a broad study of the ocular and extraocular photoreceptors of reptiles, we have used h... more As part of a broad study of the ocular and extraocular photoreceptors of reptiles, we have used high performance liquid chromatography (HPLC) to identify the retinoids present in whole eye extracts of the arboreal lizard Anolis carolinensis and the non-arboreal ruin lizard Podarcis sicula. Unexpectedly, only vitamin A2-derived chromophore was detected in Anolis, while a mixture of vitamin A1- and vitamin A2-derived chromophores was detected in Podarcis. These are the first examples of fully terrestrial vertebrates using vitamin A2-derived chromophore for visual pigment generation. Furthermore, microspectrophotometric (MSP) data for Anolis show a class of photoreceptor having a visual pigment with maximum absorbance at about 625 nm, some 40 nm further into the red than has been found in any terrestrial vertebrate examined to date.
Journal of Fish Biology, 2006
To predict spectral sensitivity, microspectrophotometry (MSP) was used to measure absorbance of p... more To predict spectral sensitivity, microspectrophotometry (MSP) was used to measure absorbance of photoreceptor cells from 15 species of Lake Malaŵ i cichlids. Each fish had one rod and at least three cone pigments. UV-sensitive pigments were common, but spectral sensitivity did not clearly correlate with feeding mode or habitat.
Journal of Comparative Physiology A: Sensory, Neural, and Behavioral Physiology, 1997
Scanning electron microscopy, immunocytochemistry, and single cell microspectrophotometry were em... more Scanning electron microscopy, immunocytochemistry, and single cell microspectrophotometry were employed to characterize the photoreceptors and visual pigments in the retina of the garter snake, Thamnophis sirtalis. The photoreceptor population was found to be comprised entirely of cones, of which four distinct types were identi®ed. About 45.5% of the photoreceptors are double cones consisting of a large principal member joined near the outer segment with a much smaller accessory member. About 40% of the photoreceptors are large single cones, and about 14.5% are small single cones forming two subtypes. The outer segments of the large single cones and both the principal and accessory members of the doubles contain the same visual pigment, one with peak absorbance near 554 nm. The small single cones contain either a visual pigment with peak absorbance near 482 nm or one with peak absorbance near 360 nm. Two classes of small single cones could be distinguished also by immunocytochemistry and scanning electron microscopy. The small single cones with the 360-nm pigment provide the garter snake with selective sensitivity to light in the near ultraviolet region of the spectrum. This ultraviolet sensitivity might be important in localization of pheromone trails.
Journal of Comparative Physiology A: Sensory, Neural, and Behavioral Physiology, 2003
Studies of visual ecology have typically focused on differences among species while paying less a... more Studies of visual ecology have typically focused on differences among species while paying less attention to variation among populations and/or individuals. Here, we show that the relative abundance of UV, violet, yellow, and red cones varies between two populations of bluefin killifish, Lucania goodei. Animals from a spring population (high-transmission UV/blue light) have a higher frequency of UV and violet cones and a lower frequency of yellow and red cones than animals from a swamp population (low-transmission UV/blue light). Visual sensitivity does not vary significantly between the populations, but spring animals tend to be more sensitive in the UV/blue wavelengths (360-440 nm) and less sensitive in longer wavelengths (560-600 nm) than swamp animals. The results have two important implications. First, the tight conservation of functional regions of opsin genes across taxa does not imply that visual systems are constrained in their evolution; differential sensitivity can arise through differential expression of cone classes within the retina. Second, intraspecific visual signals in this species may evolve to maximize contrast between the signaler and the background (as opposed to brightness); males with blue anal fins are most abundant in swamp habitats where animals express fewer UV and violet cones.
Equine Veterinary Journal, 2000
The characteristics of the ocular manifestations of equine motor neuron disease (EMND) are descri... more The characteristics of the ocular manifestations of equine motor neuron disease (EMND) are described. Forty-two horses with histories, clinical signs and necropsies compatible with EMND were the subjects of this study. Ophthalmoscopic lesions that varied in severity were found in 40 of 42 horses and appeared as a distinct pigmented reticulated pattern at the tapetal-nontapetal junction or throughout the fundus, depending upon severity. The pattern colours ranged from yellow brown to black. Areas of hyperreflectivity formed mosaic patterns in the tapetal fundus. ERG B-wave amplitudes were usually at least 50% reduced and many animals showed extinguished amplitudes. None of the horses had apparent visual impairment. Histopathologically, all 42 horses had retinal pigment epithelial (RPE) congestion with ceroid-lipofuscin. Retinal degeneration was variable even within the eyes. Thin layer chromatography (TLC) analysis of the RPE and neural retina identified both green and orange emitting fluorescent compounds not found in normal horses. All unsupplemented horses had plasma vitamin E levels <1.0 microg/ml. The potential significance of this report is the pathognomonic role the ocular manifestations exhibit in helping to diagnose equine motor neuron disease.
Journal of Molecular Evolution, 2011
Female preference for male orange coloration in the genus Poecilia suggests a role for duplicated... more Female preference for male orange coloration in the genus Poecilia suggests a role for duplicated long wavelength-sensitive (LWS) opsin genes in facilitating behaviors related to mate choice in these species. Previous work has shown that LWS gene duplication in this genus has resulted in expansion of long wavelength visual capacity as determined by microspectrophotometry (MSP). However, the relationship between LWS genomic repertoires and expression of LWS retinal cone classes within a given species is unclear. Our previous study in the related species, Xiphophorus helleri, was the first characterization of the complete LWS opsin genomic repertoire in conjunction with MSP expression data in the family Poeciliidae, and revealed the presence of four LWS loci and two Electronic supplementary material The online version of this article (
Journal of the American Veterinary Medical Association, 1985
ABSTRACT NCAR's Electra Doppler radar (ELDORA) with a dual-beam slotted waveguide array u... more ABSTRACT NCAR's Electra Doppler radar (ELDORA) with a dual-beam slotted waveguide array using dual-transmitter, dual-beam, rapid scan and step-chirped waveform significantly improved the spatial scale to 300m (Hildebrand et al. 1996). However, ELDORA X-band radar's penetration into precipitation is limited by attenuation and is not designed to collect polarimetric measurements to remotely estimate microphysics. ELDORA has been placed on dormancy because its airborne platform (P3 587) was retired in January 2013. The US research community has strongly voiced the need to continue measurement capability similar to the ELDORA. A critical weather research area is quantitative precipitation estimation/forecasting (QPE/QPF). In recent years, hurricane intensity change involving eye-eyewall interactions has drawn research attention (Montgomery et al., 2006; Bell and Montgomery, 2006). In the case of convective precipitation, two issues, namely, (1) when and where convection will be initiated, and (2) determining the organization and structure of ensuing convection, are key for QPF. Therefore collocated measurements of 3-D winds and precipitation microphysics are required for achieving significant skills in QPF and QPE. Multiple radars in dual-Doppler configuration with polarization capability estimate dynamical and microphysical characteristics of clouds and precipitation are mostly available over land. However, storms over complex terrain, the ocean and in forest regions are not observable by ground-based radars (Bluestein and Wakimoto, 2003). NCAR/EOL is investigating potential configurations for the next generation airborne radar that is capable of retrieving dynamic and microphysical characteristics of clouds and precipitation. ELDORA's slotted waveguide array radar is not compatible for dual-polarization measurements. Therefore, the new design has to address both dual-polarization capability and platform requirements to replace the ELDORA system. NCAR maintains a C-130 aircraft in its fleet for airborne atmospheric measurements, including dropsonde, and in situ sampling and remote sensing of clouds, chemistry and aerosols. Therefore, the addition of a precipitation radar to the NSF/NCAR C-130 platform will produce transformational change in its mission. This new design can be cloned for C-130s operated by a number of agencies, including NOAA and the Air Force hurricane reconnaissance fleet. This paper presents a possible configuration of a novel, airborne phased array radar (APAR) to be installed on the NSF/NCAR C-130 aircraft with improved spatial resolution and polarimetric capability to meet or exceed that of ELDORA. The preliminary design, an update of the APAR project, and a future plan will be presented. References: Bell, M. M. , M. T. Montgomery, 2008: Observed Structure, Evolution, and Potential Intensity of Category 5 Hurricane Isabel (2003) from 12 to 14 September. Monthly Weather Review, Vol. 136, Issue 6, pp. 2023-2046. Hildebrand, P. H., W.-C. Lee, C. A. Walther, C. Frush, M. Randall, E. Loew, R. Neitzel, R. Parsons, J. Testud, F. Baudin, and A. LeCornec, 1996: The ELDORA/ASTRAIA airborne Doppler weather radar: High resolution observations from TOGA COARE. Bull. Amer. Metoro. Soc., 77, 213-232 Howard B. Bluestein, Roger M. Wakimoto, 2003: Mobile Radar Observations of Severe Convective Storms re Convective Storms. Meteorological Monographs, Vol. 30, Issue 52, pp. 105-105. Montgomery, M. T., M. M. Bell, S. D. Aberson, M. L. Black, 2006: Hurricane Isabel (2003): New Insights into the Physics of Intense Storms. Part I: Mean Vortex Structure and Maximum Intensity Estimates. Bull. of the American Meteorl. Soc., Vol. 87, Issue 10, pp. 1335-1347.
Visual Neuroscience, 1996
Retinas of the nocturnal geckos, Hemidactylus turcicus, Hemidactylus garnotii, and Teratoscincus ... more Retinas of the nocturnal geckos, Hemidactylus turcicus, Hemidactylus garnotii, and Teratoscincus scincus, were studied with microspectrophotometry and immunocytochemistry against various visual pigment epitopes to reveal UV-sensitive photoreceptors. From 6-20% of the thinner members of type C double photoreceptors, earlier believed to be blue-sensitive, were found to contain a UV-absorbing visual pigment with lambda max at 363-366 nm. The pigment had bleaching and dichroic properties typical of other photoreceptor cell types of the retina. Presumptive UV-sensitive cells in retinal sections were "negatively" labeled as they did not react with either the cone-specific monoclonal antibody COS-1 or with the anti-rhodopsin polyclonal serum AO, which together labeled all of the remaining photoreceptor types (green-sensitive A singles, B doubles, and thicker members of C doubles, as well as the blue-sensitive majority of thinner members of C doubles). UV cells were moderately stained with the mAb K42-41 produced against the 5-6 loop of bovine rhodopsin, which also moderately labeled blue-sensitive cells. mAb OS-2 strongly stained all outer segments, including the UV-sensitive ones. Similarities between gecko UV visual pigments, and UV visual pigments of other vertebrates, as well as possible functional significance of these cells are discussed.
Visual Neuroscience, 2001
Absorbance spectra of cone outer segments and oil droplets were recorded microspectrophotometrica... more Absorbance spectra of cone outer segments and oil droplets were recorded microspectrophotometrically in the retina of the red-eared turtle, Trachemys scripta elegans. There are four cone visual pigments, with lambda(max) = 617 nm (red sensitive), 515 nm (green sensitive), 458 nm (blue sensitive), and 372 nm (UV-sensitive). The red-sensitive pigment resides in single cones with red or orange oil droplets, and in both members of double cones. The principal member of the double cone contains an orange oil droplet, and the accessory member is droplet free. The green-sensitive pigment is situated in single cones with orange/dark yellow droplets. The blue-sensitive pigment is combined with the UV-absorbing oil droplet in single cones. The UV-sensitive pigment resides in single cones with clear oil droplets that exhibited virtually no absorbance down to 325 nm. Thus, seven types of cones can be identified based on their morphology, oil droplet color, and the visual pigment absorbance. At the moment, this is the most complex cone system described for vertebrates.
Vision Research, 1992
As part of a broad study of the ocular and extraocular photoreceptors of reptiles, we have used h... more As part of a broad study of the ocular and extraocular photoreceptors of reptiles, we have used high performance liquid chromatography (HPLC) to identify the retinoids present in whole eye extracts of the arboreal lizard Anolis carolinensis and the non-arboreal ruin lizard Podarcis sicula. Unexpectedly, only vitamin A2-derived chromophore was detected in Anolis, while a mixture of vitamin A1- and vitamin A2-derived chromophores was detected in Podarcis. These are the first examples of fully terrestrial vertebrates using vitamin A2-derived chromophore for visual pigment generation. Furthermore, microspectrophotometric (MSP) data for Anolis show a class of photoreceptor having a visual pigment with maximum absorbance at about 625 nm, some 40 nm further into the red than has been found in any terrestrial vertebrate examined to date.
Journal of Fish Biology, 2006
To predict spectral sensitivity, microspectrophotometry (MSP) was used to measure absorbance of p... more To predict spectral sensitivity, microspectrophotometry (MSP) was used to measure absorbance of photoreceptor cells from 15 species of Lake Malaŵ i cichlids. Each fish had one rod and at least three cone pigments. UV-sensitive pigments were common, but spectral sensitivity did not clearly correlate with feeding mode or habitat.
Journal of Comparative Physiology A: Sensory, Neural, and Behavioral Physiology, 1997
Scanning electron microscopy, immunocytochemistry, and single cell microspectrophotometry were em... more Scanning electron microscopy, immunocytochemistry, and single cell microspectrophotometry were employed to characterize the photoreceptors and visual pigments in the retina of the garter snake, Thamnophis sirtalis. The photoreceptor population was found to be comprised entirely of cones, of which four distinct types were identi®ed. About 45.5% of the photoreceptors are double cones consisting of a large principal member joined near the outer segment with a much smaller accessory member. About 40% of the photoreceptors are large single cones, and about 14.5% are small single cones forming two subtypes. The outer segments of the large single cones and both the principal and accessory members of the doubles contain the same visual pigment, one with peak absorbance near 554 nm. The small single cones contain either a visual pigment with peak absorbance near 482 nm or one with peak absorbance near 360 nm. Two classes of small single cones could be distinguished also by immunocytochemistry and scanning electron microscopy. The small single cones with the 360-nm pigment provide the garter snake with selective sensitivity to light in the near ultraviolet region of the spectrum. This ultraviolet sensitivity might be important in localization of pheromone trails.
Journal of Comparative Physiology A: Sensory, Neural, and Behavioral Physiology, 2003
Studies of visual ecology have typically focused on differences among species while paying less a... more Studies of visual ecology have typically focused on differences among species while paying less attention to variation among populations and/or individuals. Here, we show that the relative abundance of UV, violet, yellow, and red cones varies between two populations of bluefin killifish, Lucania goodei. Animals from a spring population (high-transmission UV/blue light) have a higher frequency of UV and violet cones and a lower frequency of yellow and red cones than animals from a swamp population (low-transmission UV/blue light). Visual sensitivity does not vary significantly between the populations, but spring animals tend to be more sensitive in the UV/blue wavelengths (360-440 nm) and less sensitive in longer wavelengths (560-600 nm) than swamp animals. The results have two important implications. First, the tight conservation of functional regions of opsin genes across taxa does not imply that visual systems are constrained in their evolution; differential sensitivity can arise through differential expression of cone classes within the retina. Second, intraspecific visual signals in this species may evolve to maximize contrast between the signaler and the background (as opposed to brightness); males with blue anal fins are most abundant in swamp habitats where animals express fewer UV and violet cones.
Equine Veterinary Journal, 2000
The characteristics of the ocular manifestations of equine motor neuron disease (EMND) are descri... more The characteristics of the ocular manifestations of equine motor neuron disease (EMND) are described. Forty-two horses with histories, clinical signs and necropsies compatible with EMND were the subjects of this study. Ophthalmoscopic lesions that varied in severity were found in 40 of 42 horses and appeared as a distinct pigmented reticulated pattern at the tapetal-nontapetal junction or throughout the fundus, depending upon severity. The pattern colours ranged from yellow brown to black. Areas of hyperreflectivity formed mosaic patterns in the tapetal fundus. ERG B-wave amplitudes were usually at least 50% reduced and many animals showed extinguished amplitudes. None of the horses had apparent visual impairment. Histopathologically, all 42 horses had retinal pigment epithelial (RPE) congestion with ceroid-lipofuscin. Retinal degeneration was variable even within the eyes. Thin layer chromatography (TLC) analysis of the RPE and neural retina identified both green and orange emitting fluorescent compounds not found in normal horses. All unsupplemented horses had plasma vitamin E levels <1.0 microg/ml. The potential significance of this report is the pathognomonic role the ocular manifestations exhibit in helping to diagnose equine motor neuron disease.
Journal of Molecular Evolution, 2011
Female preference for male orange coloration in the genus Poecilia suggests a role for duplicated... more Female preference for male orange coloration in the genus Poecilia suggests a role for duplicated long wavelength-sensitive (LWS) opsin genes in facilitating behaviors related to mate choice in these species. Previous work has shown that LWS gene duplication in this genus has resulted in expansion of long wavelength visual capacity as determined by microspectrophotometry (MSP). However, the relationship between LWS genomic repertoires and expression of LWS retinal cone classes within a given species is unclear. Our previous study in the related species, Xiphophorus helleri, was the first characterization of the complete LWS opsin genomic repertoire in conjunction with MSP expression data in the family Poeciliidae, and revealed the presence of four LWS loci and two Electronic supplementary material The online version of this article (