L rahbarnia - Academia.edu (original) (raw)
I am an assistant professor of medical biotechnology in medical university of Tabriz,Iran.
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Papers by L rahbarnia
Journal of Analytical Research in Clinical Medicine, Jul 25, 2013
Asian Journal of Animal and Veterinary Advances, 2012
Enzyme Research, 2011
Parasite of the genusLeishmaniais reliant on the salvage pathway for recycling of ribonucleotides... more Parasite of the genusLeishmaniais reliant on the salvage pathway for recycling of ribonucleotides. A class I nuclease enzyme also known as P4 nuclease is involved in salvage of purines in cutaneous Leishmania species but the relevant enzymes have not been characterized inLeishmania infantum(L. infantum). The aim of this study was to clone and characterize the gene encoding class I nuclease inL. infantum. DNA extracted fromL. infantumwas used for amplification of P4 nuclease gene (Li-P4) by PCR. The product was cloned, sequenced, and expressed inE. colifor further characterization. Analysis of the sequence of Li-P4 revealed that the gene consists of an ORF of 951 bp. Sequence similarity analysis indicated that Li-P4 has a high homology to relevant enzymes of other kintoplastids with the highest homology (88%) to p1/s1 class I nuclease fromL. donovani. Western blotting of antirecombinant Li-P4 with promastigote and amastigote stages ofL. infantumshowed that this nuclease is present in...
Archives of Razi Institute, 2021
Brucellosis is recognized as a zoonotic disease with high morbidity in the absence of treatment. ... more Brucellosis is recognized as a zoonotic disease with high morbidity in the absence of treatment. The primary diagnosis of brucellosis can be effective in the achievement of satisfying treatment results and prevention of chronic infections. The present study aimed to compare the efficiency of conventional microbiological and serological approaches with nested Polymerase chain reaction (nested PCR) for rapid diagnosis of human brucellosis. A total of 120 subjects with symptoms of brucellosis were included in the study. The sensitivity and specificity of nested PCR for the detection of Brucella bacteria were compared with serological and blood culture methods. Out of 120 patients enrolled, brucellosis was detected in 73 (60.83%) cases based on serological tests with a blood culture confirmation in 8.33% of participants. Based on the obtained results, 55% of cases were positive in serum agglutination test (SAT≥1:160), and Coombs (C-SAT≥1:160) tests. Furthermore, seven negative SAT cases...
Journal of Analytical Research in Clinical Medicine, Jul 25, 2013
Asian Journal of Animal and Veterinary Advances, 2012
Enzyme Research, 2011
Parasite of the genusLeishmaniais reliant on the salvage pathway for recycling of ribonucleotides... more Parasite of the genusLeishmaniais reliant on the salvage pathway for recycling of ribonucleotides. A class I nuclease enzyme also known as P4 nuclease is involved in salvage of purines in cutaneous Leishmania species but the relevant enzymes have not been characterized inLeishmania infantum(L. infantum). The aim of this study was to clone and characterize the gene encoding class I nuclease inL. infantum. DNA extracted fromL. infantumwas used for amplification of P4 nuclease gene (Li-P4) by PCR. The product was cloned, sequenced, and expressed inE. colifor further characterization. Analysis of the sequence of Li-P4 revealed that the gene consists of an ORF of 951 bp. Sequence similarity analysis indicated that Li-P4 has a high homology to relevant enzymes of other kintoplastids with the highest homology (88%) to p1/s1 class I nuclease fromL. donovani. Western blotting of antirecombinant Li-P4 with promastigote and amastigote stages ofL. infantumshowed that this nuclease is present in...
Archives of Razi Institute, 2021
Brucellosis is recognized as a zoonotic disease with high morbidity in the absence of treatment. ... more Brucellosis is recognized as a zoonotic disease with high morbidity in the absence of treatment. The primary diagnosis of brucellosis can be effective in the achievement of satisfying treatment results and prevention of chronic infections. The present study aimed to compare the efficiency of conventional microbiological and serological approaches with nested Polymerase chain reaction (nested PCR) for rapid diagnosis of human brucellosis. A total of 120 subjects with symptoms of brucellosis were included in the study. The sensitivity and specificity of nested PCR for the detection of Brucella bacteria were compared with serological and blood culture methods. Out of 120 patients enrolled, brucellosis was detected in 73 (60.83%) cases based on serological tests with a blood culture confirmation in 8.33% of participants. Based on the obtained results, 55% of cases were positive in serum agglutination test (SAT≥1:160), and Coombs (C-SAT≥1:160) tests. Furthermore, seven negative SAT cases...