Luísa Santos - Academia.edu (original) (raw)
Papers by Luísa Santos
Revista de Estudios e Investigación en Psicología y Educación, 2017
Partimos da premissa base - a relevância atribuída ao papel da família e das instituições educati... more Partimos da premissa base - a relevância atribuída ao papel da família e das instituições educativas desde a primeira infância, como principais agentes de socialização.Este estudo exploratório tem como objetivo principal - conhecer as perceções de 18 educadoras relativamente à sexualidade, às práticas promotoras de saúde e à igualdade de género na primeira infância, facilitando a reflexão pessoal e coletiva. Recorremos à metodologia qualitativa – focus group, realizaram-se sete sessões. A análise dos resultados revelou que o discurso social e o educativo refletem sistemas de valores culturais tradicionais, que dificultam a abordagem da sexualidade na primeira infância.
Multidisciplinarity, 2018
Multidisciplinarity, 2018
Multidisciplinarity, 2018
INYI Journal, 1969
From Conflict to Conviviality through Creativity and Culture is a transnational cooperation proje... more From Conflict to Conviviality through Creativity and Culture is a transnational cooperation project, in Portugal, Sweden, Denmark, Spain, Lithuania, France, England and Germany, that responds to a need amongst European cultural institutions that share a common challenge: to explore how training and education in art and culture can constitute powerful resources to reflect on emerging forms of conflict, as well as to envision creative ways to deal with conflictual phenomena, while contributing to audience development. As the title of the project suggests, it aims to look into the passage of conflictual situations into conviviality through the use of creativity and culture. In other words, it demands for the action of creativity and culture in a world characterised by conflict. In this article, the aim is to reflect on the project’s wishes to advance the conceptual framework of intercultural dialogue and enhance the role of public arts and cultural institutions in the promotion of toge...
PloS one, 2016
Dissolved organic matter (DOM) in estuaries derives from a diverse array of both allochthonous an... more Dissolved organic matter (DOM) in estuaries derives from a diverse array of both allochthonous and autochthonous sources. In the estuarine system Ria de Aveiro (Portugal), the seasonality and the sources of the fraction of DOM that absorbs light (CDOM) were inferred using its optical and fluorescence properties. CDOM parameters known to be affected by aromaticity and molecular weight were correlated with physical, chemical and meteorological parameters. Two sites, representative of the marine and brackish water zones of the estuary, and with different hydrological characteristics, were regularly surveyed along two years, in order to determine the major influences on CDOM properties. Terrestrial-derived compounds are the predominant source of CDOM in the estuary during almost all the year and the two estuarine zones presented distinct amounts, as well as absorbance and fluorescence characteristics. Freshwater inputs have major influence on the dynamics of CDOM in the estuary, in part...
FEMS Microbiology Ecology, 2011
Bacterial communities of the surface microlayer (SML) of the estuary Ria de Aveiro (Portugal) wer... more Bacterial communities of the surface microlayer (SML) of the estuary Ria de Aveiro (Portugal) were characterized in terms of abundance and activity during a 2-year survey at two sites with distinct hydrodynamic properties (marine and brackish water zones). The hydrodynamic conditions were simulated using a bidimensional numerical model and related to the microbiological observations. The pattern of variation of bacterial biomass productivity (BBP) was distinct between the two sampling sites. At the outer site, BBP was significantly lower at the SML, whereas at the inner site, it was significantly enhanced at the SML. Although the total bacterial abundance was similar in the SML and underlying water (UW), the fraction of cells attached to particles was significantly higher at the SML (two to three times). The integration of microbiological results with environmental and hydrological variables shows that strong currents in the marine zone promote the vertical mixing, inhibiting the establishment of an SML bacterial community distinct from that of UW. In contrast, in the brackish water zone, lower current velocities provide conditions for enhancing the bacterial activity in the enriched SML. Estuarine dynamics influence the distribution and activity of microorganisms at the SML and in the water column, with anticipated impacts for the carbon cycle in the estuarine environment.
Aquatic Microbial Ecology, 2011
Bacteria that degrade polycyclic aromatic hydrocarbons (PAHs) in the estuarine surface microlayer... more Bacteria that degrade polycyclic aromatic hydrocarbons (PAHs) in the estuarine surface microlayer (SML) of the Ria de Aveiro, Portugal-which is chronically polluted with oil hydrocarbons (OH)-were isolated and characterized; Pseudomonas was dominant among the PAH-degrading bacteria. Screening for PAH dioxygenase genes detected almost identical nahAc genes (encoding the alpha subunits of naphthalene dioxygenase) in 2 phylogenetically distinct isolates: Pseudomonas sp. and an unknown species of the family Enterobacteriaceae; this suggested that horizontal transfer of nah genes might be involved in PAH degradation in the SML. We also investigated the effect of PAH contamination on the spatial variability of the bacterioneuston along a gradient of pollution in the estuarine system of the Ria de Aveiro. Culture-independent techniques-fluorescence in situ hybridization (FISH) and denaturing-gradient gel electrophoresis (DGGE)-revealed a similar structure among the bacterioneuston communities along the estuary. In contrast, we detected differences in the relative abundance and diversity of organisms of the Gammaproteobacteria, including those of the genus Pseudomonas (which belongs to the Gammaproteobacteria). This is the first insight into the hydrocarbonoclastic bacterial communities in the SML of an estuarine area polluted with hydrocarbons. Our findings highlight the importance of SML-adapted hydrocarbonoclastic bacterioneuston as a potential source of new PAH-degrading bacteria (including new pseudomonads) with potential use in the bioremediation of hydrocarbon-polluted ecosystems.
Annals of Microbiology, 2013
Annals of Microbiology, 2013
ABSTRACT It is well known that there are prokaryotes small in size (e.g. ultra-microprokaryotes) ... more ABSTRACT It is well known that there are prokaryotes small in size (e.g. ultra-microprokaryotes) that pass through a 0.2-μm filter. As bacterial and viral abundances are determined by epifluorescence microscopy and the differentiation between them is based on particle size, some bacteria can be erroneously enumerated as viruses, namely in marine waters where bacteria are small. However, there is no information on the proportion of prokaryotes that could be misidentified as viruses by epifluorescence microscopy. In this work, we assessed, in water samples collected in the estuarine system Ria de Aveiro (Portugal), the proportion of prokaryotes that could be counted as viruses by the current widespread epifluorescence microscopy and, for the first time, by fluorescence in situ hybridization (FISH). The total number of particles was determined on membranes of 0.2 and 0.02 μm after staining with 4′,6-diamidino-2-phenylindole (DAPI), and the number of prokaryotes (Bacteria and Archaea) was determined by FISH for both pore size membranes. The results show that, in the marine zone of the estuarine system, 28 % of particles enumerated as virus-like particles were prokaryotes, but, in the brackish water zone, only 13 % of the particles counted as viruses were actually prokaryotic cells. Epifluorescence microscopy overestimates viral abundance, and also the ratio viruses:prokaryotes, and this error must be taken into consideration because it can vary significantly within a system. In fact, in the marine zone of an estuarine system, the overestimation of viral abundance can be twice as high as in the brackish water zone.
Revista de Estudios e Investigación en Psicología y Educación, 2017
Partimos da premissa base - a relevância atribuída ao papel da família e das instituições educati... more Partimos da premissa base - a relevância atribuída ao papel da família e das instituições educativas desde a primeira infância, como principais agentes de socialização.Este estudo exploratório tem como objetivo principal - conhecer as perceções de 18 educadoras relativamente à sexualidade, às práticas promotoras de saúde e à igualdade de género na primeira infância, facilitando a reflexão pessoal e coletiva. Recorremos à metodologia qualitativa – focus group, realizaram-se sete sessões. A análise dos resultados revelou que o discurso social e o educativo refletem sistemas de valores culturais tradicionais, que dificultam a abordagem da sexualidade na primeira infância.
Multidisciplinarity, 2018
Multidisciplinarity, 2018
Multidisciplinarity, 2018
INYI Journal, 1969
From Conflict to Conviviality through Creativity and Culture is a transnational cooperation proje... more From Conflict to Conviviality through Creativity and Culture is a transnational cooperation project, in Portugal, Sweden, Denmark, Spain, Lithuania, France, England and Germany, that responds to a need amongst European cultural institutions that share a common challenge: to explore how training and education in art and culture can constitute powerful resources to reflect on emerging forms of conflict, as well as to envision creative ways to deal with conflictual phenomena, while contributing to audience development. As the title of the project suggests, it aims to look into the passage of conflictual situations into conviviality through the use of creativity and culture. In other words, it demands for the action of creativity and culture in a world characterised by conflict. In this article, the aim is to reflect on the project’s wishes to advance the conceptual framework of intercultural dialogue and enhance the role of public arts and cultural institutions in the promotion of toge...
PloS one, 2016
Dissolved organic matter (DOM) in estuaries derives from a diverse array of both allochthonous an... more Dissolved organic matter (DOM) in estuaries derives from a diverse array of both allochthonous and autochthonous sources. In the estuarine system Ria de Aveiro (Portugal), the seasonality and the sources of the fraction of DOM that absorbs light (CDOM) were inferred using its optical and fluorescence properties. CDOM parameters known to be affected by aromaticity and molecular weight were correlated with physical, chemical and meteorological parameters. Two sites, representative of the marine and brackish water zones of the estuary, and with different hydrological characteristics, were regularly surveyed along two years, in order to determine the major influences on CDOM properties. Terrestrial-derived compounds are the predominant source of CDOM in the estuary during almost all the year and the two estuarine zones presented distinct amounts, as well as absorbance and fluorescence characteristics. Freshwater inputs have major influence on the dynamics of CDOM in the estuary, in part...
FEMS Microbiology Ecology, 2011
Bacterial communities of the surface microlayer (SML) of the estuary Ria de Aveiro (Portugal) wer... more Bacterial communities of the surface microlayer (SML) of the estuary Ria de Aveiro (Portugal) were characterized in terms of abundance and activity during a 2-year survey at two sites with distinct hydrodynamic properties (marine and brackish water zones). The hydrodynamic conditions were simulated using a bidimensional numerical model and related to the microbiological observations. The pattern of variation of bacterial biomass productivity (BBP) was distinct between the two sampling sites. At the outer site, BBP was significantly lower at the SML, whereas at the inner site, it was significantly enhanced at the SML. Although the total bacterial abundance was similar in the SML and underlying water (UW), the fraction of cells attached to particles was significantly higher at the SML (two to three times). The integration of microbiological results with environmental and hydrological variables shows that strong currents in the marine zone promote the vertical mixing, inhibiting the establishment of an SML bacterial community distinct from that of UW. In contrast, in the brackish water zone, lower current velocities provide conditions for enhancing the bacterial activity in the enriched SML. Estuarine dynamics influence the distribution and activity of microorganisms at the SML and in the water column, with anticipated impacts for the carbon cycle in the estuarine environment.
Aquatic Microbial Ecology, 2011
Bacteria that degrade polycyclic aromatic hydrocarbons (PAHs) in the estuarine surface microlayer... more Bacteria that degrade polycyclic aromatic hydrocarbons (PAHs) in the estuarine surface microlayer (SML) of the Ria de Aveiro, Portugal-which is chronically polluted with oil hydrocarbons (OH)-were isolated and characterized; Pseudomonas was dominant among the PAH-degrading bacteria. Screening for PAH dioxygenase genes detected almost identical nahAc genes (encoding the alpha subunits of naphthalene dioxygenase) in 2 phylogenetically distinct isolates: Pseudomonas sp. and an unknown species of the family Enterobacteriaceae; this suggested that horizontal transfer of nah genes might be involved in PAH degradation in the SML. We also investigated the effect of PAH contamination on the spatial variability of the bacterioneuston along a gradient of pollution in the estuarine system of the Ria de Aveiro. Culture-independent techniques-fluorescence in situ hybridization (FISH) and denaturing-gradient gel electrophoresis (DGGE)-revealed a similar structure among the bacterioneuston communities along the estuary. In contrast, we detected differences in the relative abundance and diversity of organisms of the Gammaproteobacteria, including those of the genus Pseudomonas (which belongs to the Gammaproteobacteria). This is the first insight into the hydrocarbonoclastic bacterial communities in the SML of an estuarine area polluted with hydrocarbons. Our findings highlight the importance of SML-adapted hydrocarbonoclastic bacterioneuston as a potential source of new PAH-degrading bacteria (including new pseudomonads) with potential use in the bioremediation of hydrocarbon-polluted ecosystems.
Annals of Microbiology, 2013
Annals of Microbiology, 2013
ABSTRACT It is well known that there are prokaryotes small in size (e.g. ultra-microprokaryotes) ... more ABSTRACT It is well known that there are prokaryotes small in size (e.g. ultra-microprokaryotes) that pass through a 0.2-μm filter. As bacterial and viral abundances are determined by epifluorescence microscopy and the differentiation between them is based on particle size, some bacteria can be erroneously enumerated as viruses, namely in marine waters where bacteria are small. However, there is no information on the proportion of prokaryotes that could be misidentified as viruses by epifluorescence microscopy. In this work, we assessed, in water samples collected in the estuarine system Ria de Aveiro (Portugal), the proportion of prokaryotes that could be counted as viruses by the current widespread epifluorescence microscopy and, for the first time, by fluorescence in situ hybridization (FISH). The total number of particles was determined on membranes of 0.2 and 0.02 μm after staining with 4′,6-diamidino-2-phenylindole (DAPI), and the number of prokaryotes (Bacteria and Archaea) was determined by FISH for both pore size membranes. The results show that, in the marine zone of the estuarine system, 28 % of particles enumerated as virus-like particles were prokaryotes, but, in the brackish water zone, only 13 % of the particles counted as viruses were actually prokaryotic cells. Epifluorescence microscopy overestimates viral abundance, and also the ratio viruses:prokaryotes, and this error must be taken into consideration because it can vary significantly within a system. In fact, in the marine zone of an estuarine system, the overestimation of viral abundance can be twice as high as in the brackish water zone.