Luciana Honorato - Academia.edu (original) (raw)

Papers by Luciana Honorato

Ciência Rural, 2014

O objetivo deste trabalho foi adaptar e testar uma metodologia participativa para promover e aval... more O objetivo deste trabalho foi adaptar e testar uma metodologia participativa para promover e avaliar as mudanças de percepção e de atitude dos agricultores com relação às práticas de manejo sanitário da ordenha, através da construção e uso de indicadores que permitissem aos agricultores um processo de ação-avaliação constante. Foi realizado um estudo de caso em quatro Unidades de Produção Familiar (UPF), no município de Guaraciaba (SC), envolvidas na atividade leiteira. Após a caracterização das UPF e diagnóstico sanitário do rebanho, foi feita a construção participativa de indicadores de manejo sanitário de ordenha, a qual foi utilizada pelos agricultores ao longo de três meses para avaliação de suas práticas de manejo. Ao final desse período, foram realizadas entrevistas com as famílias participantes para apreender a sua percepção a respeito da metodologia empregada. Os agricultores avaliaram positivamente o uso da metodologia, destacando a melhoria de vários indicadores, devido à...

F1000Research

Background: Aloe barbadensis Miller, also known as Aloe vera is a well-known phytotherapeutic, an... more Background: Aloe barbadensis Miller, also known as Aloe vera is a well-known phytotherapeutic, and parts of its leaves are used for a wide range of medicinal purposes. This study seeks to assess the in vitro antimicrobial and cytotoxic effects of leaf exudate (LE) from A. barbadensis leaves against Staphylococcus aureus and MAC-T bovine mammary epithelial cells. Methods: Seasonal LE samples were collected, and the effect on total phenolic and aloin contents was determined. Antimicrobial activity of LE was evaluated using the broth microdilution method, and toxicity to MAC-T cells was determined by MTT assay. Results: Samples collected during different seasons of the year showed a seasonal effect on the chemical profile of LE (P<0.05). However, despite these chemical variations, we found no differences in antimicrobial activity against S. aureus. For all studied samples, the minimum inhibitory concentration (MIC) was 1,000 µg/mL. Furthermore, we found an elevated cytotoxic effect ...

Journal of Dairy Science, 2014

The aims of this study were to investigate the environmental, feeding, and health management of o... more The aims of this study were to investigate the environmental, feeding, and health management of organic (ORG) family dairy farms in the south of Brazil in comparison with conventional (CONV) farms, and to assess their degree of compliance with Brazilian organic legislation and the strategies they adopt to accomplish this (n = 17 per group). During 2 visits to each farm in March and September, 2010, observations were made on the environment, feed, and health management, followed by bulk milk testing, clinical evaluation, and breed assessment of each individual cow, and an evaluation of diseases and treatments reported within the period. Additional data were collected directly from the farmers through direct interviews. The number of lactating cows was, on average, 11 (range 5 to 19) in the ORG and 16 (range 7 to 42) in the CONV herds. The ORG herds presented a lower percentage of the Holstein breed; whereas CONV herds were predominantly Holstein, in the ORG herds, only 2 herds were 100% Holstein and the remaining herds were crosses of Holstein, Jersey, and Gir (Bos indicus) cattle. Milk production per cow was lower (10.2 vs. 15.1 ± 1.22 L/cow, respectively) in ORG than in the CONV farms. The ORG farms offered less concentrate feed than CONV farms and had better pasture management. Organic farmers reported using phytotherapic and homeopathic products, and pasture management as a strategy to keep infection levels of endo-and ectoparasites low, whereas CONV farmers regularly used anthelmintics and acaricides. Milk production was lower in ORG than in CONV farms, but cow health and condition scores were broadly similar, indicating that the with these strategies ORG farms were able to secure levels of animal welfare comparable with CONV farms while complying with organic regulation, although at the cost of lower cow productivity.

Animals, 2015

Pasture-based dairy production is used widely on family dairy farms in Southern Brazil. This stud... more Pasture-based dairy production is used widely on family dairy farms in Southern Brazil. This study investigates conventional high input (C-HI), conventional low input (C-LI), and organic low input (O-LI) pasture-based systems and their effects on quantity and quality of the milk produced. We conducted technical site visits and interviews monthly over one year on 24 family farms (n = 8 per type). C-HI farms had the greatest total area (28.9 ha), greatest percentage of area with annual pasture (38.7%), largest number of lactating animals (26.2) and greatest milk yield per cow (22.8 kg·day(-1)). O-LI farms had the largest perennial pasture area (52.3%), with the greatest botanical richness during all seasons. Area of perennial pasture was positively correlated with number of species consumed by the animals (R² = 0.74). Milk from O-LI farms had higher levels of fat and total solids only during the winter. Hygienic and microbiological quality of the milk was poor for all farms and need to be improved. C-HI farms had high milk yield related to high input, C-LI had intermediate characteristics and O-LI utilized a year round perennial pasture as a strategy to diminish the use of supplements in animal diets, which is an important aspect in ensuring production sustainability.

Journal of the Science of Food and Agriculture, 2014

Phenolic compounds are widely present in forage. However, few studies have been carried out to in... more Phenolic compounds are widely present in forage. However, few studies have been carried out to investigate the presence of these compounds in animal products such as milk. In this study, the total phenolic content (TPC) and ferric reducing-antioxidant power (FRAP) of milk produced under agroecological and conventional management systems in southern Brazil were determined. Three systems were compared: (a) agroecological, with intensive rotational grazing, also known as Voisin grazing (ECO); (b) semi-intensive conventional (SIC); and (c) conventional grazing (CGR) (n = 8 per group). Pastures with distinct and more diverse botanical composition were observed on the ECO farms. Significantly (P &amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;lt; 0.05) lower amounts of TPC were found in agroecologically produced milk in autumn and summer and the CGR system showed the highest FRAP value (P &amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;lt; 0.05) for the samples collected in autumn. Positive correlations where found between the TPC and FRAP values obtained for the milk samples (0.198, P &amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;lt; 0.05), milk FRAP and forage TPC values (0.344, P &amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;lt; 0.05), and the TPC and FRAP values obtained for the forage (0.70, p &amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;lt; 0.01). PCA applied to the UV spectra dataset (200-350 nm) clearly distinguished the samples collected from the ECO system in the winter. Our results revealed that, under the conditions prevalent in southern Brazil, since the cows were allowed to graze in all of the systems, the TPC and antioxidant capacity of the milk samples showed minimal variation. However, since the forage TPC and FRAP values for the milk were correlated, TPC appears to be a promising variable for the purpose of monitoring forage prior to its selection aimed at enhancing the antioxidant activity of milk.

Revista Brasileira De Agroecologia, Apr 24, 2014

RESUMO: É crescente o número de produtores interessados no sistema de produção orgânico, especial... more RESUMO: É crescente o número de produtores interessados no sistema de produção orgânico, especialmente dentro da Agricultura Familiar. Porém, pouco se sabe sobre a estruturação das unidades produtivas, tecnologias e práticas que estão sendo utilizadas nesse sistema. O objetivo desse trabalho foi identificar o perfil de produtores de leite orgânico através da caracterização das famílias e unidades produtivas, investigar as motivações, as principais dificuldades e aspectos positivos e negativos da produção orgânica, além de comparar características demográficas, produtivas e manejos utilizados nos sistemas orgânico e convencional. Foram selecionadas 17 unidades leiteiras orgânicas (ORG) e 17 unidades leiteiras convencionais (CONV), de agricultores familiares, no Oeste de Santa Catarina, Brasil. Foram realizadas entrevistas com os agricultores em março e setembro de 2010 sobre suas opiniões a respeito da produção orgânica, e sobre as diferenças entre as tecnologias e manejos adotados. O tamanho da propriedade, idade, escolaridade e tempo na atividade foram similares entre os sistemas. O ORG teve rebanhos menores e menor produção por vaca, o que foi relacionado à menor porcentagem de sangue holandês no rebanho e não ao sistema em si. Esse sistema apresentou uma produção leiteira menor do que o CONV com médias de 3342 e 7912 litros/mês, respectivamente. O ORG teve um perfil de pessoas mais ativas no seu meio social, tendo maior acesso a informações, o que pode ter influenciado na opção pelo sistema de produção. A melhor exploração dos recursos forrageiros e menor utilização de antibióticos fazem com que os produtores orgânicos percebam melhorias no agroecossistema e na qualidade de vida. O maior entrave percebido pelos produtores orgânicos é a falta de reconhecimento econômico pelo mercado e de assistência técnica especializada no assunto. A adequação dos produtores aos padrões normativos é limitada pela falta de planejamento alimentar e de saúde animal. PALAVRAS-CHAVE: leite orgânico; agricultura familiar; processo de transição.

Ciência Rural

Changes in the quality of interactions between animals and humans have a profound influence on th... more Changes in the quality of interactions between animals and humans have a profound influence on the productivity and welfare of animals and, potentially, of humans involved in the dairy activity. Important alterations in these interactions can be obtained through training programs, or other forms of extension. To do so effectively, it is necessary to understand how interact the several aspects of this relationship, which includes besides the animals humans, and the environment where they are inserted. Most of published studies confirm the model of positive feedback human behavior and attitudes, and animal behavior. However, these studies have been carried out mostly in European countries or Australia, mostly under intensive, confined systems, and usually with the aiming of indicating an ideal worker profile. In this review we show that the rearing system may exert a strong influence in this process and conclude suggesting the development of studies with the objective of understanding...

Journal of dairy science, Jan 23, 2015

The objective of this study was to evaluate in vitro the antimicrobial activity of Brazilian prop... more The objective of this study was to evaluate in vitro the antimicrobial activity of Brazilian propolis from Urupema, São Joaquim, and Agua Doce (Santa Catarina State) and green propolis from Minas Gerais State, and the effects of propolis on bovine mammary gland explant viability. The propolis samples differed in flavonoid content and antioxidant activity. Green propolis showed the highest content of flavonoids, followed by the sample from São Joaquim. The propolis from Urupema showed the lowest flavonoid content along with the lowest antioxidant activity. The total phenolics were similar across all studied samples. Despite phytochemical differences, the propolis samples from Minas Gerais, São Joaquim, and Urupema presented the same level of antimicrobial activity against Staphylococcus aureus strains. The reduction in S. aureus growth was, on average, 1.5 and 4 log10 times at 200 and 500 μg/mL, respectively. At concentrations of 1,000 μg/mL, all propolis reduced bacterial growth to ...