Luciana Retz Carvalho - Profile on Academia.edu (original) (raw)

Papers by Luciana Retz Carvalho

Fen�is halogenados e/ou sulfatados de macroalgas marinhas

Quim Nova, 2000

Estudos taxonômicos in vitro e prospecção de toxinas de cianobactérias isoladas de reservatórios do estado de São Paulo

raibt.net.br

... cianobactérias isoladas de reservatórios do estado de São Paulo Raquel Ieda Lopes (1) ,Lucian... more ... cianobactérias isoladas de reservatórios do estado de São Paulo Raquel Ieda Lopes (1) ,Luciana Retz de Carvalho (2) , Andréa Tucci (2) ... Biomagnification of cyanobacterial neurotoxins and neurodegeneraive disease among the Chamorro people of Guam. ...

Revista Brasileira de Plantas Medicinais, 2014

Hypericum cordatum é uma espécie do cerrado que foi selecionada em triagem de plantas com ativida... more Hypericum cordatum é uma espécie do cerrado que foi selecionada em triagem de plantas com atividade fungitóxica. O objetivo do presente trabalho foi isolar e identificar compostos com atividade antifúngica em extratos de folhas em diclorometano. O pó das folhas das plantas foi submetido à extração exaustiva com éter de petróleo e diclorometano. O extrato em diclorometano, e as frações ativas, foram submetidos à fracionamentos biomonitorados em coluna de Sephadex LH-20, respectivamente, com os eluentes clorofórmio:metanol (1:1) e com um gradiente de hexano:diclorometano (1:4); diclorometano:acetona (3:2 e 1:4), metanol, e água. As frações que mostraram atividade foram submetidas à cromatografia em camada delgada preparativa de sílica gel GF254, sendo que o material de maior massa foi analisado em CLAE semipreparativa. A fração ativa foi analisada por RMN de ¹H, tendo sido identificado o aloaromadendrano - 4α -10β - diol como componente principal da fração. Conclui-se, portanto, que e...

Brazilian Journal of Botany, 2018

Radiocystis fernandoi Komárek and Komárková-Legnerová has great ecological importance in tropical... more Radiocystis fernandoi Komárek and Komárková-Legnerová has great ecological importance in tropical regions, due to its widespread occurrence in freshwaters in the American continent, besides its ability to form blooms and to produce toxins. Global warming has a very important and positive role on cyanobacterial development considering that the growth rate of these organisms increases at temperatures above 25°C. Considering that there is no information in the literature about the effects of temperature on the development of R. fernandoi, we performed an experimental study on its effects on growth and microcystin-LR production, employing a tropical microcystin-producer R. fernandoi. The selected strain was tested at three temperatures: 20, 25 and 30°C and sampled at intervals of 2 days including different growth phases. Our results show that the strain presents greater growth rates and the smaller cell size at 25 and 30°C. Differently from Microcystis aeruginosa (Kützing) Kützing that presents the greatest microcystin production at the end of the exponential growth phase, R. fernandoi produced microcystin only in the stationary phase. This result emphasizes the importance of monitoring Radiocystis blooms for a long period of time considering that these toxins remain in the water for up to 42 days. Thus, besides the morphological differences between M. aeruginosa and R. fernandoi, our results reveal that physiologically these cyanobacterial species also present different strategies that allow them to co-occur in nature forming long-lasting blooms.

Article Analysis of the Toxicity and Histopathology Induced by the Oral Administration of Pseudanabaena galeata and Geitlerinema splendidum (Cyanobacteria) Extracts to Mice

marine drugs

Revista de Biología Marina y Oceanografía, 2020

Macroalgae are considered bioindicators for marine pollution, because they have the ability to qu... more Macroalgae are considered bioindicators for marine pollution, because they have the ability to quickly react to changes in their environment. In consequence, macroalgae populations fluctuate, according to species characteristics and adaptive strategies. Their cell wall polysaccharides contain sulfate groups that are capable of retaining and accumulating heavy metals. In addition to traditional contaminants, emerging pollutants are being recognized in aquatic environments. Herein, emerging pollutants have been identified after being desorbed from the macroalga Dichotomaria marginata, collected from Fortaleza Beach, Ubatuba -SP, Brazil. Based on that algal polysaccharide networks have the potential of forming hydrogen bonds with polar compounds, it was hypothesized that these pollutants would be bound to sugar polymers. Compounds present in the D. marginata samples were identified using both gas and liquid chromatography/mass spectrometry (GC/MS and HPLC/MS), assisted by computational...

Efeito inibitório de extratos de cianobactérias frente ao vírus Herpes simplex tipo 1

Xiv Congresso Brasileiro De Ficologia, May 28, 2012

Infeccoes causadas pelo virus herpes simplex tipo 1 (HSV-1) incidem sobre consideravel parcela d... more Infeccoes causadas pelo virus herpes simplex tipo 1 (HSV-1) incidem sobre consideravel parcela da populacao humana mundial, atingindo indices de ate 90%. Nao existe cura para a infeccao pelo HSV-1, cujas cepas, por vezes, sao resistentes as terapias existentes. Cyanobacteria tem se mostrado potencial fonte de farmacos o que levou-nos, neste estudo, a avaliacao da citotoxicidade e da atividade inibitoria, frente ao HSV-1, dos extratos metanolicos (EM) e em acido acetico 0,1M (EHAc) das cianobacterias Rhabdoderma sp. CCIBt773, Geitlerinema unigranulatum CCIBt971 e Phormidium sp. CCIBt1018. Para detectar alteracoes morfologicas celulares, determinar a concentracao maxima nao toxica (CMNT) e o efeito na viabilidade celular (CC 50 ) dos diferentes extratos, foram empregadas celulas Vero. Foram determinados os titulos virais, o indice de inibicao viral (IIV) e a porcentagem de Inibicao (PI) dos extratos. A partir das curvas dose-resposta, foram calculados as concentracoes capazes de inibir em 50% a replicacao viral (EC 50 ) e o indice de seletividade (IS). A toxicidade para celulas Vero (alteracoes morfologicas) variou entre 62,5 e 500mg/mL, nao sendo observada diferenca significativa entre os extratos EM e EHAc. Quanto a viabilidade, com excecao do EM de CCIBt971, que apresentou CC 50 =455µg/mL, todos os extratos mostraram CC 50 superiores a maior concentracao avaliada (500µg/mL). O EM de CCIBt971 inibiu em 99,9%, com EC 50 =25µg/mL e IS=18,2 e o EHAc, em 60,2%, com EC 50 =26µg/mL e IS>8,7. O EM de CCIBt773, inibiu em 99,8% a propagacao viral com EC 50 =141µg/mL e IS>3,5, enquanto o EHAc inibiu em 82,2%, com EC 50 =34µg/mL e IS >14,7. Em relacao a CCIBt1018, o EM mostrou inibicao de 82,2%, com EC 50 =52µg/mL e IS > 9,6 e o EHAc apresentou inibicao de 96% e EC 50 =383µg/mL e IS>1,3. Cinco dos seis extratos inibiram em mais de 80% a propagacao viral; este e os demais resultados obtidos indicam o potencial efeito anti HSV-1 destes extratos e justificam a continuacao destes estudos.

Histopathological evaluation of injected mice with extracts of two toxic cyanobacteria strains

Revista Brasileira de Farmacognosia, 2011

In Brazil, the Laurencia complex is represented by twenty taxa: Laurencia s.s. with twelve specie... more In Brazil, the Laurencia complex is represented by twenty taxa: Laurencia s.s. with twelve species, Palisada with four species (including Chondrophycus furcatus now that the proposal of its transference to Palisada is in process), and Osmundea and Yuzurua with two species each. The majority of the Brazilian species of the Laurencia complex have been phylogenetically analyzed by 54 rbcL sequences, including five other Rhodomelacean species as outgroups. The analysis showed that the Laurencia complex is monophyletic with high posterior probability value. The complex was separated into five clades, corresponding to the genera: Chondrophycus, Laurencia, Osmundea, Palisada, and Yuzurua. A bibliographical survey of the terpenoids produced by Brazilian species showed that only six species of Laurencia and five of Palisada (including C. furcatcus) have been submitted to chemical analysis with 48 terpenoids (47 sesquiterpenes and one triterpene) isolated. No diterpenes were found. Of the total, 23 sesquiterpenes belong to the bisabolane class and eighteen to the chamigrene type, whose biochemical precursor is bisabolane, two are derived from lauranes and four are triquinols. Despite the considerable number of known terpenes and their ecological and pharmacological importance, few experimental biological studies have been performed. In this review, only bioactivities related to human health were considered.

Revista Brasileira de Botânica, 2007

-(Cyanobacterial occurrence and detection of microcystins by planar chromatography in surface wat... more -(Cyanobacterial occurrence and detection of microcystins by planar chromatography in surface water of Billings and Guarapiranga Reservoirs, SP, Brazil). Billings and Guarapiranga Reservoirs were deeply affected by environmental disturbances, which more evident consequence are the cyanobacterial blooms. Microcystins are the most common cyanotoxin in freshwaters and more than 70 types are known. Different methods for microcystins analysis in water can be used, among which ELISA and HPLC are the most frequently employed. However, less sophisticated and more economic methods can also be used. This is the case of planar chromatography (thin-layer chromatography) method previously used in cyanotoxins purification but gradually replaced by others. Posterior optimization of the microcystin chromatography conditions and because of its simplicity, rapidity, efficiency and low cost, this method is again considered an option for the analysis of microcystins and nodularins. Considering the importance of Billings and Guarapiranga Reservoirs for drinking water supplies and the few scientific data about cyanobacteria and cyanotoxins in these water bodies, the aims of this work are to analyze the biodiversity of cyanobacteria in the Billings and Guarapiranga Reservoirs and the detection of dissolved microcystins in the water. It was possible to identify 17 species of cyanobacteria, 9 of them being potentially toxic. In Billings Reservoir Microcystis aeruginosa (Kützing) Kützing and Cylindrospermopsis raciborskii (Woloszynska) Seenayya & Subba Raju are the most common species, while in Guarapiranga Reservoir only M. aeruginosa was considered as a common species. Microcystins were detected in all Billings Reservoir samples and in only one sample from Guarapiranga Reservoir. Key words-cyanobacteria, drinking water, microcystins, planar chromatography, reservoirs RESUMO-(Ocorrência de cianobactérias e detecção por cromatografia planar de microcistinas dissolvidas nas águas superficiais das represas Billings e de Guarapiranga, SP, Brasil). As represas Billings e Guarapiranga foram profundamente afetadas por fatores ambientais cuja conseqüência mais evidente são as florações de cianobactérias. As microcistinas formam a classe de cianotoxinas mais freqüente em água doce e são comumente analisadas por ELISA ou CLAE. No entanto, processos menos sofisticados e mais econômicos também podem ser usados. Este é o caso da cromatografia planar (cromatografia em camada delgada), método anteriormente usado em trabalhos de purificação de cianotoxinas, mas que foi gradualmente substituído por outros. Assim, considerando a importância das represas Billings e Guarapiranga para o abastecimento público e a carência de informações científicas sobre as cianobactérias e as cianotoxinas que nelas ocorrem, nossos objetivos foram o estudo da composição desses organismos e a detecção de microcistina dissolvida na água. Foi possível identificar 19 espécies de cianobactérias, das quais 9 são consideradas potencialmente tóxicas. Microcystis aeruginosa (Kützing) Kützing e Cylindrospermopsis raciborskii (Woloszynska) Seenayya & Subba Raju são as espécies mais comuns no Reservatório Billings e, na Guarapiranga, apenas M. aeruginosa foi considerada como espécie de ocorrência comum. Microcistina-LR foi detectada em todas as amostras da Represa Billings e em somente uma amostra da Represa Guarapiranga. Palavras-chave-água de abastecimento, cianobactérias, cromatografia planar, microcistinas, reservatórios

Marine Drugs, 2014

Cyanobacteria are common members of the freshwater microbiota in lakes and drinking water reservo... more Cyanobacteria are common members of the freshwater microbiota in lakes and drinking water reservoirs, and are responsible for several cases of human intoxications in Brazil. Pseudanabaena galeata and Geitlerinema splendidum are examples of the toxic species that are very frequently found in reservoirs in Sao Paulo, which is the most densely populated area in Brazil. In the search for toxic strains collected from water reservoirs and maintained in the Cyanobacterial Culture Collection (CCIBt) of the Institute of Botany of Brazil, the acetic acid extracts (AE) of P. galeata CCIBt 3082 and G. splendidum CCIBt 3223 were analyzed by planar chromatography, which indicated the absence of cyanotoxins. Animal tests were then carried out, and both extracts were found to induce toxic effects in mice when administered intraperitoneally. The present study aimed to investigate whether the oral ingestion of the above mentioned cyanobacteria extracts would also induce toxic effects in mice. Necropsy and histopathological studies were conducted using tissue samples from the animals, which were euthanized one week after the administration of the extracts. The AE of P. galeata did not cause death but did induce transient symptoms, including eyebrow ptosis, straub tail, and pain. The euthanized

Revista Brasileira de Farmacognosia, 2013

An investigation was directed towards the antiacetylcholinesterase activity of the acid aqueous a... more An investigation was directed towards the antiacetylcholinesterase activity of the acid aqueous and methanolic extracts of fi ve cyanobacterial taxa, which encompasses an enzymatic inhibition essay and the evaluation of the physiological responses of mice to cyanobacterial extracts along with toxicological observations. The strains Calothrix sp. CCIBt 3320, Tolypothrix sp. CCIBt 3321, Phormidium cf. amoenum CCIBt 3412, Phormidium sp. CCIBt 3265, and Geitlerinema splendidum CCIBt 3223 were from the São Paulo Botanical Institute Cyanobacterial Culture Collection and all of them showed inhibitory effect on acetylcholinesterase activity (in vitro) and caused systemic effects similar to those described for anticholinesterase drugs (in vivo). With the exception of G. splendidum and Tolypothrix sp. strains, all extracts produced reversible antiacetylcolinesterase effects in mice. Complementary histopathological studies were carried out on tissues from animals administered with Phormidium sp. and P. cf. amoenum.

Toxicon : official journal of the International Society on Toxinology, 2011

Toxic cyanobacteria in public water reservoirs may cause severe health issues for livestock and h... more Toxic cyanobacteria in public water reservoirs may cause severe health issues for livestock and human beings. Geitlerinema amphibium, which is frequently found in São Paulo City's drinking water supplies, showed toxicity in the standard mouse bioassay, while displaying signs of intoxication and post-mortem findings different from those showed by animals intoxicated by known cyanotoxins. We report here the alterations caused by G. amphibium methanolic extract on mouse microcirculatory network, as seen by in vivo intravital microscopy, besides observations on leukocyte migration, cytokine quantitation, and results of toxicological essays. Our data showed that G. amphibium methanolic extract displayed time- and dose-dependent pro-inflammatory activity, and that at lower doses [125 and 250 mg/kg body weight (b.w.)] increased the leukocyte rolling, caused partial venular stasis, as well as induced an increase in leukocyte counts in the peripheral blood and peritoneal washings. At hi...

Review of toxic species of Cyanobacteria in Brazil

Algological Studies, 2008

Page 1. Review of toxic species of Cyanobacteria in Brazil CÉLIA L. SANT&amp;#x27;ANNA1, ... more Page 1. Review of toxic species of Cyanobacteria in Brazil CÉLIA L. SANT&amp;#x27;ANNA1, MARIA TERESA DE P. AZEVEDO1, VERA R. WERNER2, CAMILA R. DOGO1, FERNANDA R. RIOS1 and LUCIANA R. DE CARVALHO1 1Instituto ...

Toxicon

Toxic cyanobacteria in public water reservoirs may cause severe health issues for livestock and h... more Toxic cyanobacteria in public water reservoirs may cause severe health issues for livestock and human beings. Geitlerinema amphibium, which is frequently found in São Paulo City's drinking water supplies, showed toxicity in the standard mouse bioassay, while displaying signs of intoxication and post-mortem findings different from those showed by animals intoxicated by known cyanotoxins. We report here the alterations caused by G. amphibium methanolic extract on mouse microcirculatory network, as seen by in vivo intravital microscopy, besides observations on leukocyte migration, cytokine quantitation, and results of toxicological essays. Our data showed that G. amphibium methanolic extract displayed time-and dose-dependent pro-inflammatory activity, and that at lower doses [125 and 250 mg/kg body weight (b.w.)] increased the leukocyte rolling, caused partial venular stasis, as well as induced an increase in leukocyte counts in the peripheral blood and peritoneal washings. At higher doses (500 and 1000 mg/kg b.w.), the extract caused ischemic injury leading to animal death. As confirmed by mass spectrometric studies and polymyxin B test, the G. amphibium methanolic extract did not contain lipopolysaccharides.

Neurotoxicity Research, 2011

While evaluating several laboratory-cultured cyanobacteria strains for the presence of paralytic ... more While evaluating several laboratory-cultured cyanobacteria strains for the presence of paralytic shellfish poison neurotoxins, the hydrophilic extract of Microcystis aeruginosa strain SPC777—isolated from Billings’s reservoir, São Paulo, Brazil—was found to exhibit lethal neurotoxic effect in mouse bioassay. The in vivo test showed symptoms that unambiguously were those produced by PSP. In order to identify the presence of neurotoxins, cells were lyophilized, and the extracts were analyzed by HPLC–FLD and HPLC–MS. HPLC–FLD analysis revealed four main Gonyautoxins: GTX4(47.6%), GTX2(29.5%), GTX1(21.9%), and GTX3(1.0%). HPLC–MS analysis, on other hand, confirmed both epimers, with positive Zwitterions M+ 395.9 m/z for GTX3/GTX2 and M+ 411 m/z for GTX4/GTX1 epimers. The hepatotoxins (Microcystins) were also evaluated by ELISA and HPLC–MS analyses. Positive immunoreaction was observed by ELISA assay. Alongside, the HPLC–MS analyses revealed the presence of [l-ser7] MCYST-RR. The N-methyltransferase (NMT) domain of the microcystin synthetase gene mcyA was chosen as the target sequence to detect the presence of the mcy gene cluster. PCR amplification of the NMT domain, using the genomic DNA of the SPC777 strain and the MSF/MSR primer set, resulted in the expected 1,369 bp product. The phylogenetic analyses grouped the NMT sequence with the NMT sequences of other known Microcystis with high bootstrap support. The taxonomical position of M. aeruginosa SPC777 was confirmed by a detailed morphological description and a phylogenetic analysis of 16S rRNA gene sequence. Therefore, co-production of PSP neurotoxins and microcystins by an isolated M. aeruginosa strain is hereby reported for the first time.

Brazilian Journal of Microbiology, 2008

Reports of cyanobacterial blooms developing worldwide have considerably increased, and, in most c... more Reports of cyanobacterial blooms developing worldwide have considerably increased, and, in most cases, the predominant toxins are microcystins. The present study reports a cyanobacterial bloom in Lake Violão, Torres, Rio Grande do Sul State, in January 2005. Samples collected on January 13, 2005, were submitted to taxonomical, toxicological, and chemical studies. The taxonomical analysis showed many different species of cyanobacteria, and that Microcystis protocystis and Sphaerocavum cf. brasiliense were dominant. Besides these, Microcystis panniformis, Anabaena oumiana,Cylindrospermopsis raciborskii, and Anabaenopsis elenkinii f. circularis were also present. The toxicity of the bloom was confirmed through intraperitoneal tests in mice, and chemical analyses of bloom extracts showed that the major substance was anabaenopeptin F, followed by anabaenopeptin B, microcystin-LR, and microcystin-RR.

Review of toxic species of Cyanobacteria in Brazil

Algological Studies, 2008

Toxicon

Toxic cyanobacteria in public water reservoirs may cause severe health issues for livestock and h... more Toxic cyanobacteria in public water reservoirs may cause severe health issues for livestock and human beings. Geitlerinema amphibium, which is frequently found in São Paulo City's drinking water supplies, showed toxicity in the standard mouse bioassay, while displaying signs of intoxication and post-mortem findings different from those showed by animals intoxicated by known cyanotoxins. We report here the alterations caused by G. amphibium methanolic extract on mouse microcirculatory network, as seen by in vivo intravital microscopy, besides observations on leukocyte migration, cytokine quantitation, and results of toxicological essays. Our data showed that G. amphibium methanolic extract displayed time-and dose-dependent pro-inflammatory activity, and that at lower doses [125 and 250 mg/kg body weight (b.w.)] increased the leukocyte rolling, caused partial venular stasis, as well as induced an increase in leukocyte counts in the peripheral blood and peritoneal washings. At higher doses (500 and 1000 mg/kg b.w.), the extract caused ischemic injury leading to animal death. As confirmed by mass spectrometric studies and polymyxin B test, the G. amphibium methanolic extract did not contain lipopolysaccharides.

Neurotoxicity Research, 2011

Fen�is halogenados e/ou sulfatados de macroalgas marinhas

Quim Nova, 2000

Estudos taxonômicos in vitro e prospecção de toxinas de cianobactérias isoladas de reservatórios do estado de São Paulo

raibt.net.br

Revista Brasileira de Plantas Medicinais, 2014

Brazilian Journal of Botany, 2018

Article Analysis of the Toxicity and Histopathology Induced by the Oral Administration of Pseudanabaena galeata and Geitlerinema splendidum (Cyanobacteria) Extracts to Mice

marine drugs

Revista de Biología Marina y Oceanografía, 2020

Efeito inibitório de extratos de cianobactérias frente ao vírus Herpes simplex tipo 1

Xiv Congresso Brasileiro De Ficologia, May 28, 2012

Histopathological evaluation of injected mice with extracts of two toxic cyanobacteria strains

Revista Brasileira de Farmacognosia, 2011

Revista Brasileira de Botânica, 2007

Marine Drugs, 2014

Revista Brasileira de Farmacognosia, 2013

Toxicon : official journal of the International Society on Toxinology, 2011

Review of toxic species of Cyanobacteria in Brazil

Algological Studies, 2008

Toxicon

Toxic cyanobacteria in public water reservoirs may cause severe health issues for livestock and h... more Toxic cyanobacteria in public water reservoirs may cause severe health issues for livestock and human beings. Geitlerinema amphibium, which is frequently found in São Paulo City's drinking water supplies, showed toxicity in the standard mouse bioassay, while displaying signs of intoxication and post-mortem findings different from those showed by animals intoxicated by known cyanotoxins. We report here the alterations caused by G. amphibium methanolic extract on mouse microcirculatory network, as seen by in vivo intravital microscopy, besides observations on leukocyte migration, cytokine quantitation, and results of toxicological essays. Our data showed that G. amphibium methanolic extract displayed time-and dose-dependent pro-inflammatory activity, and that at lower doses [125 and 250 mg/kg body weight (b.w.)] increased the leukocyte rolling, caused partial venular stasis, as well as induced an increase in leukocyte counts in the peripheral blood and peritoneal washings. At higher doses (500 and 1000 mg/kg b.w.), the extract caused ischemic injury leading to animal death. As confirmed by mass spectrometric studies and polymyxin B test, the G. amphibium methanolic extract did not contain lipopolysaccharides.

Neurotoxicity Research, 2011

Brazilian Journal of Microbiology, 2008

Review of toxic species of Cyanobacteria in Brazil

Algological Studies, 2008

Toxicon

Toxic cyanobacteria in public water reservoirs may cause severe health issues for livestock and h... more Toxic cyanobacteria in public water reservoirs may cause severe health issues for livestock and human beings. Geitlerinema amphibium, which is frequently found in São Paulo City's drinking water supplies, showed toxicity in the standard mouse bioassay, while displaying signs of intoxication and post-mortem findings different from those showed by animals intoxicated by known cyanotoxins. We report here the alterations caused by G. amphibium methanolic extract on mouse microcirculatory network, as seen by in vivo intravital microscopy, besides observations on leukocyte migration, cytokine quantitation, and results of toxicological essays. Our data showed that G. amphibium methanolic extract displayed time-and dose-dependent pro-inflammatory activity, and that at lower doses [125 and 250 mg/kg body weight (b.w.)] increased the leukocyte rolling, caused partial venular stasis, as well as induced an increase in leukocyte counts in the peripheral blood and peritoneal washings. At higher doses (500 and 1000 mg/kg b.w.), the extract caused ischemic injury leading to animal death. As confirmed by mass spectrometric studies and polymyxin B test, the G. amphibium methanolic extract did not contain lipopolysaccharides.

Neurotoxicity Research, 2011