Luciano Casagrande - Academia.edu (original) (raw)

Papers by Luciano Casagrande

Journal of dentistry for children, 2020

Purpose: To assess the survival and risk factors associated with the failure of atraumatic restor... more Purpose: To assess the survival and risk factors associated with the failure of atraumatic restorative treatment (ART) restorations placed in children with early child-hood caries (ECC). Methods: The sample consisted of 286 restorations from the records of 137 children treated in a university dental clinic. The date of restoration placement, any reintervention (failure), and the patient's last checkup were recorded. Restoration longevity up to one year of follow-up was assessed via the Kaplan-Meier survival test. Multi-variate Cox regression analysis with shared frailty was used to evaluate the factors associated with failures (P <0.05). Results: The mean age of the children was 2.3±0.6 years, presenting a decayed, missing, and filled teeth (dmft) mean of 6.3±3.2. Mean survival time was 11.1 months (95 percent confidence interval = 10.8 to 11.4), with 85 percent of the restorations surviving after one year. Caries experience was associated with risk of restoration failure in ...

Revista da ABENO, 2020

Este estudo investigou o ensino do reparo de restauração direta de resina composta em dentes decí... more Este estudo investigou o ensino do reparo de restauração direta de resina composta em dentes decíduos nos cursos de graduação em Odontologia no Brasil. Um questionário referente a este tópico foi desenvolvido e enviado por e-mail para 205 cursos de Odontologia entre maio e setembro de 2019. Os dados obtidos foram analisados descritivamente. A taxa de resposta foi de 43,4% e dos cursos respondentes, 82% incluíram esse tópico em seu currículo. As duas razões mais comumente relatadas para o ensino do reparo de restaurações de resina composta foram preservação da estrutura dentária (95,9%) e redução do risco de complicações pulpares (71,2%). No que diz respeito ao protocolo para reparo, poucos instituições (24,7%) ensinaram o desgaste mecânico da porção da resina a ser reparada com pontas diamantadas. Por outro lado, o condicionamento com ácido fosfórico da superfície preparada foi recomendado por 87,7% das instituições e 76,7% indicaram aplicação de adesivo na superfície preparada. O ...

Revista da Faculdade de Odontologia de Porto Alegre, 2012

Objetivo: Este trabalho teve como propósito verificar a associação entre o tipo e o tempo de amam... more Objetivo: Este trabalho teve como propósito verificar a associação entre o tipo e o tempo de amamentação fornecida ao bebê com a presença de hábitos de respiração bucal, bruxismo, mordida aberta anterior e presença de processos respiratórios alérgicos. Materiais e métodos: Foram analisados todos os prontuários clínicos de crianças entre 0 a 12 anos de idade atendidos nos ambulatórios odontológicos do Centro Universitário Franciscano (UNIFRA), no período de 2005 a 2010. De um total de 507 prontuários, foram incluídos no estudo prontuários de 390 crianças. Resultados: Os resultados demonstraram haver associação entre o tipo de amamentação com a presença de respiração bucal, bruxismo e mordida aberta anterior (p

The Journal of Contemporary Dental Practice, 2009

Aim The aim of this paper is to present a review and discussion of the current status of stem cel... more Aim The aim of this paper is to present a review and discussion of the current status of stem cell research with regard to tooth generation. Background Stem cells have been isolated from the pulp tissue of both deciduous and permanent teeth as well as from the periodontal ligament. Dental pulp stem cells demonstrate the capacity to form a dentin pulp-like complex in immunocompromised mice. A tooth-like structure was successfully formed, using a heterogeneous mixture of dental enamel epithelium, pulp mesenchymal cells, and scaffolds. Conclusion The scientific community understands the need for more investigations to completely understand the conditions that would best favor the creation of a tooth substitute. Recent gains in the understanding of the molecular regulation of tooth morphogenesis, stem cell biology, and biotechnology offers the opportunity to realize this goal. Clinical Significance These findings, combined with the recent progress in stem cell research and tissue engine...

The Journal of the American Dental Association, 2019

Clinical oral investigations, Jan 7, 2018

Cultivation under hypoxia promotes different responses in the mesenchymal stem cells and it has b... more Cultivation under hypoxia promotes different responses in the mesenchymal stem cells and it has been producing promising results for clinical applications. Pulp tissue from deciduous teeth is a source of stem cells which has a high proliferative potential but this is usually discarded. This study has evaluated the effects of hypoxia on proliferation, apoptosis, and the expression of the pluripotency-related genes of the stem cells from human exfoliated deciduous teeth (SHED). The cells were isolated from dental pulp (n = 5) and characterized as mesenchymal stem cells, in accordance with the International Society for Cell Therapy. The cells were cultivated under hypoxia (3% oxygen) and compared to the normoxia cells (21% oxygen). The proliferation rate was evaluated by the Ki67 antibody for up to 7 days, while the metabolic activity was measured by the wst-8 assay for up to 14 days. The apoptotic cells were analyzed by Annexin V and propidium iodide staining at 24 h and 4 and 7 days....

American journal of dentistry, 2018

To evaluate the longevity and factors associated with failure of primary teeth restorations place... more To evaluate the longevity and factors associated with failure of primary teeth restorations placed in high caries-risk children. The sample was comprised of children treated in a University Dental Service. Patients' records were screened retrospectively to determine whether they had received restorative treatment in primary teeth presenting cavitated caries lesions. Kaplan-Meier estimator and Multivariate Cox regression analysis with shared frailty were used to assess restorations' survival and factors associated with failure, respectively. 123 high caries-risk children (10.3±4 DMF-T) with 316 restorations were analyzed. The 3-year survival reached 53.4% (AFR=18.8%). Restorations placed without rubber dam (P= 0.04), over selective caries removal (P= 0.03), with calcium hydroxide liner (P< 0.01) and glass-ionomer cement (P= 0.04) presented lower survival rates. Caries-controlled patients presented significantly (P= 0.03) higher rates of restoration survival (77.7%) than ca...

Clinical oral investigations, Jan 22, 2016

The aim of this retrospective university-based study has been to evaluate the longevity and facto... more The aim of this retrospective university-based study has been to evaluate the longevity and factors associated with failures of adhesive restorations performed in deep carious lesions of permanent molars after complete (CCR) and selective caries removal (SCR). The sample was composed of composite resin and resin-modified glass ionomer cement (RMGIC) restorations placed in permanent molars of children attending a university dental service who were followed up for up to 36 months. Information collected retrospectively from clinical records was used for analyzing data. The following factors were investigated: gender, caries experience, visible plaque and gingival bleeding indexes, operator's experiences, number of restored surfaces, and type of capping and restorative materials. The Kaplan-Meier survival test was used to analyze the longevity of the restorations. Multivariate Cox regression analysis with shared frailty was used to assess the factors associated with failures (p <...

International Endodontic Journal, 2015

Journal of Clinical Pediatric Dentistry, 2014

Objectives: To isolate cells from pulp of intact cryopreserved deciduous teeth. The null hypothes... more Objectives: To isolate cells from pulp of intact cryopreserved deciduous teeth. The null hypothesis raised here is to find no difference in the establishment of cell culture after cryopreservation (1) using culture medium supplemented with different concentrations of fetal bovine serum (FBS); and (2) between teeth with different stages of physiological root resorption. Study design: Intact deciduous teeth with different root resorption stages were cryopreserved using FBS and Dimethyl Sulfoxide (DMSO) medium (9:1) in a progressive freezing process, by placing the samples in the refrigerator (4°C/60 min) and subsequently transferring them to a -80°C freezer (controlled device -1°C/min/24 hours), and finally into liquid nitrogen (-196°C/30 days). After the thawing process, the cell isolation was performed by enzymatic digestion (type I collagenase). The cells were re-suspended into the culture medium with 10% (G1) or 20% (G2) of FBS. Microscopic analysis was performed after 30 days to ...

Journal of Clinical Pediatric Dentistry, 2008

Objective: To evaluate the clinical and radiographic outcomes (24 months) of indirect pulp treatm... more Objective: To evaluate the clinical and radiographic outcomes (24 months) of indirect pulp treatment (IPT)in primary teeth when a self-etching primer or a calcium hydroxide layer was used over the remaining carious dentin. Study design: Primary molar teeth with deep carious lesions without signs and symptoms of irreversible pulpitis were divided into two groups, according to the capping material: Experimental group(1): self-etching adhesive system (Clearfill SE Bond); and Control group (2): calcium hydroxide liner(Dycal). Both groups were followed up after application of a resin restoration (3M - Z250). Results: After 2 years of clinical and radiographic follow-up, no statistical difference was found between groups (p=1). The overall success rate reached 87%. Conclusion: These results demonstrate that IPT has a high clinical and radiographic performance in primary teeth and is not dependent on the capping material used over the demineralized dentin.

Journal of Clinical Pediatric Dentistry, 2007

Stem cells are undifferentiated cells that have the capacity to self-renew. They have been discov... more Stem cells are undifferentiated cells that have the capacity to self-renew. They have been discovered in many adult tissues, including teeth. The Dental Pulp Stem Cells are involved in dentinal repair by activation of growth factors, released after caries process and have the ability to regenerate the dentin-pulp-like complex. The molecular/cellular research raises the possibilities to grow new tissues and biological structures for clinical application, providing cells for therapies including cell transplantation and tissue engineering.

Regenerative Medicine, 2014

Tissue engineering is a multidisciplinary science that aims to develop biological substitutes for... more Tissue engineering is a multidisciplinary science that aims to develop biological substitutes for tissues and organs in order to restore their functions in cases of injuries and deformities. In recent years, dentistry has explored the potential of tissue engineering through basic and translational research in order to be able to repair oral tissue damaged by biological substitutes. This new science is based on the balanced use of responsive cells, capable of differentiating in tissue of interest; biocompatible matrices, those that will support cell growth and mimic the extracellular matrix; and bioactive molecules responsible for morphogenetic signals. Currently, stem cell research has grown significantly, due to the recognition that therapies based on stem cells have the potential for treating patients under different conditions, such as bone tissue deficiency and ischemic heart disease, and even in severe conditions, such as Alzheimer’s disease and leukemia. Current evidence has demonstrated that stem cells are found in certain niches and some tissues contain more stem cells than others. Since the discovery that dental tissue can become a source of mesenchymal stem cells, a vast field of research has opened and there are promising opportunities for regenerative therapies. Due to its favorable characteristics, such as low risk of tumor formation, sources of relatively easy access, and lower ethical issues related to their use, stem cells from dental tissue are being considered as promising for clinical use in both medical and dental applications. The areas, such as endodontics, periodontics, oral surgery, and dental implants, are increasingly exploring the potential of dental tissue engineering in search of better and more advanced treatment options for their patients. Thus, this chapter aims to introduce the basic concepts of tissue bioengineering in dentistry, focusing on the isolation methods and characteristics presented by stem cells from dental tissue as well as its potential use in cell-based therapy.

ABSTRACT Objective: To evaluate the effect of dentin and scaffold porogen on in vitro and in vivo... more ABSTRACT Objective: To evaluate the effect of dentin and scaffold porogen on in vitro and in vivo dental pulp stem cells (DPSC) differentiation in odontoblasts-like cells and pulp-like tissue formation in vivo. Methods: Dentin disks were prepared from extracted human third molars. Poly-L(lactic) acid (PLLA) scaffolds were prepared in the pulp chamber using salt crystals or gelatin spheres as the porogen and PLLA scaffolds without dentin disks were used as control. Dentin was treated with EDTA, DPSC cells were seeded in the scaffolds and cultured for up to 28 days. RNA was extracted and the expression of DSPP, DMP1, and MEPE (putative odontoblastic markers) was evaluated by RT-PCR. Cell proliferation was evaluated using the WST1 assay, and cell distribution evaluated by SEM and confocal microscopy. In vivo, tooth slices/scaffolds or control scaffolds containing DPSC were implanted subcutaneously in immunodeficient mice. After 28 days, they were retrieved fixed, and evaluated by hematoxylin/eosin staining and by immunohistochemistry for Factor VIII to identify microvessels, and RT-PCR for DSPP, DMP1, and MEPE to evaluate differentiation of the cells in vivo. One-way ANOVA was used for statistical evaluation of data. Results: DPSC seeded in scaffolds/tooth slices expressed the odontoblast markers, but not those in PLLA scaffolds without the tooth slices. Cell proliferation was reduced in the presence of dentin as compared to control PLLA scaffolds (p<0.05). DPSC inside tooth slices produced a tissue with anatomical characteristics similar to those of a normal dental pulp, and expressed odontoblast markers. The type of porogen used here, i.e. gelatin spheres or salt crystals, did not affect DPSC proliferation, expression of odontoblastic markers, and pulp-like tissue formation in vivo. Conclusion: Bioactive molecules released from the dentin induce the expression of markers of odontoblastic differentiation by DPSC. Supported by the University of Michigan CRSE department and by CAPES and CNPq- Brazil.

Brazilian Oral Research, 2009

American journal of dentistry, 2006

To evaluate the bond strength and micromorphology of the fracture pattern of adhesive composite r... more To evaluate the bond strength and micromorphology of the fracture pattern of adhesive composite restorations in primary teeth after long-term clinical function. Subjects (8-10 years-old) with deep carious lesions in primary molars had their teeth restored with resin composite (Z250). The teeth were randomly divided into two groups, according to the adhesive system used: (1) Scotchbond Multi-Purpose (SMP); and (2) Clearfil SE Bond (CSE). After the clinical and radiographic follow-up period (15-17 months), the teeth were exfoliated and the adhesive restorations were subjected to microtensile bond test. Student's t-test revealed a statistical difference (P= 0.001) between the two adhesive systems tested (SMP = 16.8 +/- 2.62 MPa; CSE = 27.3 +/- 2.28 MPa). The micromorphology evaluation of the failure zone revealed a weak area in the demineralized dentin (SMP) and at the top of the hybrid layer (CSE).

American journal of dentistry, 2010

To evaluate clinical and radiographic outcomes of indirect pulp treatment (IPT) in primary molars... more To evaluate clinical and radiographic outcomes of indirect pulp treatment (IPT) in primary molars after long-term function (up to 60 months). Teeth with deep carious lesions without signs and symptoms of irreversible pulpitis were divided by random allocation into two groups, according to the capping material utilized over demineralized dentin: experimental group (1): self-etching adhesive system (Clearfil SE Bond); and control group (2): calcium hydroxide liner (Dycal). Both groups were filled with resin composite (Z250) and submitted to a clinical and radiographic monitoring period until exfoliation. After the follow-up period (up to 60 months), no statistical difference was found between groups (P= 0.514). The overall success rate reached 78%. The failures occurred after the first year period recall.

Archives of Oral Biology, 2014

Journal of Endodontics, 2010