Luis Camargo - Academia.edu (original) (raw)
Papers by Luis Camargo
Revista da Sociedade Brasileira de Medicina Tropical, 2011
INTRODUCTION: Mansonelliasis is caused by Mansonella ozzardi. It is widespread in the Amazon regi... more INTRODUCTION: Mansonelliasis is caused by Mansonella ozzardi. It is widespread in the Amazon region, with a high prevalence. The common exam of thick blood smears stained with Giemsa shows low efficacy levels and has been an obstacle to diagnosing individuals with low blood parasitemia. METHODS: In order to increase diagnosis efficacy, the PCR technique was improved. RESULTS AND CONCLUSIONS: PCR demonstrated the best performance, with sensitivity and negative predictive values (NPV) of 100%, followed by blood filtration through membrane filters, which showed a sensitivity of 88.9% and a NPV of 84.6%, when compared to thick blood smears.
The Journal of Immunology, 2010
In many hemolytic disorders, such as malaria, the release of free heme has been involved in the t... more In many hemolytic disorders, such as malaria, the release of free heme has been involved in the triggering of oxidative stress and tissue damage. Patients presenting with severe forms of malaria commonly have impaired regulatory responses. Although intriguing, there is scarce data about the involvement of heme on the regulation of immune responses. In this study, we investigated the relation of free heme and the suppression of anti-inflammatory mediators such as PGE 2 and TGF-b in human vivax malaria. Patients with severe disease presented higher hemolysis and higher plasma concentrations of Cu/Zn superoxide dismutase (SOD-1) and lower concentrations of PGE 2 and TGF-b than those with mild disease. In addition, there was a positive correlation between SOD-1 concentrations and plasma levels of TNF-a. During antimalaria treatment, the concentrations of plasma SOD-1 reduced whereas PGE 2 and TGF-b increased in the individuals severely ill. Using an in vitro model with human mononuclear cells, we demonstrated that the heme effect on the impairment of the production of PGE 2 and TGF-b partially involves heme binding to CD14 and depends on the production of SOD-1. Aside from furthering the current knowledge about the pathogenesis of vivax malaria, the present results may represent a general mechanism for hemolytic diseases and could be useful for future studies of therapeutic approaches.
Clinics, 2005
PURPOSE: This study was carried out in Monte Negro (state of Rondônia), a village in the Brazilia... more PURPOSE: This study was carried out in Monte Negro (state of Rondônia), a village in the Brazilian western Amazon region, where a University of São Paulo Medical School program for medical student training in rural assistance took place. It aimed to determine the ...
Memorias do Instituto Oswaldo Cruz, Jul 1, 2017
The sandfly Trichophoromyia auraensis has recently evolved as a proven vector of Leishmania (Vian... more The sandfly Trichophoromyia auraensis has recently evolved as a proven vector of Leishmania (Viannia) endemic to state of Acre in the north of Brazil. This note is intended to propose a correction in the report of the first occurrence of natural infection of Leishmania (Viannia) in this species. We and the other scientific groups reinforced that Tr. auraensis is a possible vector involved in the transmission of American cutaneous leishmaniasis in Acre, Brazil.
Brazilian Journal of Otorhinolaryngology, 2015
Introduction: Mucosal leishmaniosis (ML) is a severe clinical form of leishmaniosis. Complex fact... more Introduction: Mucosal leishmaniosis (ML) is a severe clinical form of leishmaniosis. Complex factors related to the parasite and the host are attributed to the development of mucosal lesions. Leishmania RNA virus 1 (LRV1) can disrupt immune response, and may be the main determinant of severity of the disease; it should be investigated. Objective: To study the existence of clinical differences between patients with ML with endosymbiosis by LRV1 and. those without it. Methods: A cross-sectional cohort study with clinical evaluation, polymerase chain reaction (PCR) detection of Leishmania, species classification, and search of LRV1 was performed. Only patients with confirmed diagnosis of ML by positive PCR and with nasal mucosa injuries were included in this analysis. Results: Out of 37 patients, 30 (81.1%) were diagnosed with Leishmania braziliensis, five (13.5%) with Leishmania guyanensis, and two (5.4%) with mixed infection of L. braziliensis and L. guyanensis. LVR1 virus was present in 26 (70.3%) of the cases.
Cadernos de Saúde Pública, 2015
Este estudo objetivou identificar fatores associados à incidência de concessão de benefícios entr... more Este estudo objetivou identificar fatores associados à incidência de concessão de benefícios entre trabalhadores na atividade de carne e pescado no Brasil. Foram estudados os benefícios auxílio-doença concedidos pela Previdência Social aos empregados em carne e pescado no Brasil em 2008. As incidências por 10 mil vínculos empregatícios foram estratificadas por sexo, idade, diagnóstico, atividade econômica, unidade federada, espécie e duração dos benefícios auxílio-doença. Foram concedidos 31.913 benefícios auxílio-doença, com incidência de 788,7. O ramo abate de bovinos, equinos, ovinos e caprinos apresentou a maior incidência (1.223,2); o pescado, a maior duração (61 dias) de benefícios auxílio-doença. As mulheres apresentaram maior incidência, enquanto os homens, maior duração de benefícios auxílio-doença. Os grupos Lesões, doenças osteomusculares e transtornos mentais representaram 67,2% dos benefícios auxílio-doença. Os diagnósticos mais incidentes foram as dorsopatias, hemorrag...
Revista Brasileira de Epidemiologia, 2004
A Leishmaniose Tegumentar Americana (LTA) é uma doença causada por protozoários do gênero Leishma... more A Leishmaniose Tegumentar Americana (LTA) é uma doença causada por protozoários do gênero Leishmania, transmitida ao homem pela picada de mosquitos flebotomíneos (Ordem Diptera; Família Psychodidae; Sub-Família Phlebotominae). No Brasil existem atualmente 6 espécies de Leishmania responsáveis pela doença humana, e mais de 200 espécies de flebotomíneos implicados em sua transmissão. Trata-se de uma doença que acompanha o homem desde tempos remotos e que tem apresentado, nos últimos 20 anos, um aumento do número de casos e ampliação de sua ocorrência geográfica, sendo encontrada atualmente em todos os Estados brasileiros, sob diferentes perfis epidemiológicos. Estima-se que, entre 1985 e 2003, ocorreram 523.975 casos autóctones, a sua maior parte nas regiões Nordeste e Norte do Brasil. Neste estudo, são discutidos aspectos relacionados ao tratamento e ao controle dessa doença, assim como também as dificuldades para a implementação dessas medidas. São apontadas alternativas que passam ...
Saúde (Santa Maria), 2012
A presente revisão objetivou descrever um histórico da busca de fármacos para tratamento da Tripa... more A presente revisão objetivou descrever um histórico da busca de fármacos para tratamento da Tripanossomíase Americana, e sua situação atual. A pesquisa foi desenvolvida através de revisão bibliográfica do tipo exploratória, retrospectiva e descritiva baseada em livros e pesquisa de artigos em base de dados Scientific Electronic Library Online (Scielo), Biblioteca Virtual em Saúde (BVS) e National Institute of Health (PUBMED), utilizando os descritores: Tripanossomíase Americana, tratamento e perspectivas. Já se passaram mais de 100 anos da descoberta da doença de Chagas, e até hoje não foi desenvolvido nenhum fármaco com potencial satisfatório de cura. O medicamento ainda utilizado é o benzonidazol, utilizado em combinação com outros medicamentos. Entre os quimioterápicos o Posaconazol, é o mais forte candidato para novos tratamentos específicos da doença e, entre os fitoterápicos, citamse as espécies Banisteriopsis caapi e Psychotria viridis, utilizadas na região amazônica como alternativa.
Memórias do Instituto Oswaldo Cruz, 1993
Zootaxa
The larval stage of Amblyomma oblongoguttatum Koch is redescribed using optical and scanning elec... more The larval stage of Amblyomma oblongoguttatum Koch is redescribed using optical and scanning electron microscopy.Unfed larvae were obtained from a colony of A. oblongoguttatum originated from engorged females collected on domesticpigs from Monte Negro municipally (10°29’S, 63°32’W), State of Rondônia, Western Amazon, Brazil. Several charactersare presented including the chaetotaxy of the idiosoma, palpi and Haller’s organ, as well as morphological features of theidiosoma, gnathosoma and legs. In addition, the porotaxy (topographical and numerical patterns of integumentary struc-tures) were presented by using a new nomenclature recently proposed. The chaetotaxy of the larvae of A. oblongoguttat-um, in general, is similar to other Neotropical Amblyomma species. Three types of integumentary structures were observedon the idiosoma: lyrifissures, small glands, and large wax glands. Topographic and numerical patterns of the integumen-tary structures consisted of 5 pairs of large wax gland...
Ticks and Tick-borne Diseases, 2010
In the present study, we compared 2 methods for collecting ixodid ticks on the verges of animal t... more In the present study, we compared 2 methods for collecting ixodid ticks on the verges of animal trails in a primary Amazon forest area in northern Brazil. (i) Dragging: This method was based on passing a 1-m2 white flannel over the vegetation and checking the flannel for the presence of caught ticks every 5–10m. (ii) Visual search: This method consisted
Arquivos do Instituto Biológico
RESUMO O presente estudo determinou a prevalência de anticorpos anti-Leptospira spp. em ovinos do... more RESUMO O presente estudo determinou a prevalência de anticorpos anti-Leptospira spp. em ovinos do Município de Monte Negro, RO. Foram examinados soros de 141 ovinos de raça, idade e sexo variados provenientes de 15 fazendas, pela técnica de Soroaglutinação Microscópica. Doze (80,0%) propriedades apresentaram pelo menos um animal reagente. Títulos de anticorpos iguais ou superiores a 100 foram detectados em 47 (33,3%) animais, e os sorovares mais frequentes foram Patoc (29,7%), Autumnalis (14,8%), Pyrogenes (10,6%), Australis (4,2%), Bratislava (4,2%), Hardjo (4,2%), Icterohaemorrhagiae (4,2%), Castellonis (2,1%) e Hebdomadis (2,1%). Em 11 (23,4%) soros não foi possível a determinação do provável sorovar envolvido na reação. Alerta-se também para a possibilidade de infecção no homem, tendo em vista as características regionais de fronteira agrícola amazônica.
Zootaxa
We present here a multisource approach that takes advantage of several disciplines to address a t... more We present here a multisource approach that takes advantage of several disciplines to address a taxonomic issue. A triatomine related to Rhodnius robustus Larrousse, 1927 was recently found in the state of Rondônia, Brazil. The name Rhodnius montenegrensis n. sp. is suggested because it was found in the municipality of Monte Negro. The main differences between these two species can be detected in the female and male genitalia, but there are also noticeable differences in their eggs. Molecular analysis using PCR-RFLP technique and Bayesian inferences based on a fragment of the Cytochrome b (Cyt b) gene corroborated the morphological findings. We used this integrative approach to address the taxonomic decision for a new Rhodnius species and its relationship with others of this genus. Results obtained herein stress that morphology must be used as the major approach for obtaining phenotypic information, and molecular data should be taken as a complementary tool.
Vector borne and zoonotic diseases (Larchmont, N.Y.), Nov 17, 2018
Rhodnius montenegrensis was described in 2012. Since then, reports of the occurrence of this spec... more Rhodnius montenegrensis was described in 2012. Since then, reports of the occurrence of this species associated with palm trees near households in Rondônia have been published. This study aimed to analyze the natural infection of R. montenegrensis with trypanosomatids in the municipality of Monte Negro, Rondônia, Brazilian western Amazon. Capture of triatomines occurred in Attalea speciosa (babassu) specimens around households. Twelve of the 72 captured triatomines were identified by morphological and morphometric characters, by molecular analysis made using the PCR, DNA sequencing, and phylogenetic analyses. The intestinal content was collected from 60 of these 72 specimens. The positivity for trypanosomatids was confirmed by examining the intestinal content followed by PCR amplification of the cathepsin L-like gene specific for Trypanosoma cruzi (PCR-DTcrCatL) and Trypanosoma rangeli (PCR-DTraCatL). Of the 60 specimens analyzed by microscopy, 22 (36.7%) were positive for trypanoso...
Revista da Sociedade Brasileira de Medicina Tropical
This communication reports the occurrence of Rhodnius neglectus in the State of Acre, Brazil. An ... more This communication reports the occurrence of Rhodnius neglectus in the State of Acre, Brazil. An adult male R. neglectus specimen was collected from the Catuaba Experimental Reserve, which is located in the municipality of Senador Guiomard. This increases the number of triatomine species reported from the State of Acre to eight and is also the first report of R. neglectus from the Brazilian Western Amazon Region. The occurrence of R. neglectus is alarming because even though the species is wild, it can invade and colonize human dwellings and peridomiciles, thereby increasing Chagas disease transmission.
Revista da Sociedade Brasileira de Medicina Tropical
The occurrence of Triatoma sordida in the Brazilian Western Amazon is reported for the first time... more The occurrence of Triatoma sordida in the Brazilian Western Amazon is reported for the first time. Triatoma sordida specimens were collected from a Gallus gallus nest in a peridomiciliary area of Senador Guiomard municipality in the state of Acre. The number of triatomine species in Acre increased from six to seven with this first report of T. sordida in the Brazilian Western Amazon. The occurrence of T. sordida in Acre is of concern since it is among the most captured triatomines in peridomiciliary environments in Brazil, and carries a high potential for vector transmission.
Arquivos brasileiros de oftalmologia
Malignant lesions of the eyelid are the most common eye cancers. Although rarely lethal, late dia... more Malignant lesions of the eyelid are the most common eye cancers. Although rarely lethal, late diagnosis can lead to serious visual morbidity. Definitive diagnosis of neoplastic lesions is pathological. Indications and types of eyelid lesions in a single high-volume ophthalmic reference center in Sao Paulo, Brazil, were analyzed. The surgical pathological reports of patients who underwent eye removal procedures between January 2000 and December 2012 were retrieved from the electronic database of the Ophthalmology Department and retrospectively reviewed. Data regarding the final anatomopathological diagnosis, sex, and age were analyzed via the χ2 test with Yates' correction. Of the 1,113 eyelid tumors resected over the 13-year study period, 324 (29%) lesions were malignant. The most prevalent lesions were basal cell, squamous cell, and sebaceous gland carcinomas. The median ages of patients (females, n=165, 51%; males, n=159, 49%) with a diagnosis of basal cell, squamous cell, and...
Revista da Sociedade Brasileira de Medicina Tropical
Rhodnius stali infection by Trypanosoma rangeli is reported in this study for the first time. The... more Rhodnius stali infection by Trypanosoma rangeli is reported in this study for the first time. The triatomines were collected from the campus of the Federal University of Acre in Rio Branco, Acre, Brazil. The identification of T. rangeli was confirmed by multiplex polymerase chain reaction. The examinations of two specimens revealed R. stali infection by the epimastigote forms of T. rangeli. The encounter of R. stali infected by T. rangeli generates an alert for the state of Acre, since the simultaneous presence with Trypanosoma cruzi can make the differential diagnosis of Chagas disease difficult.
The American journal of tropical medicine and hygiene, Jan 8, 2018
The treatment of mansonelliasis is still a challenge because there are few clinical trials for th... more The treatment of mansonelliasis is still a challenge because there are few clinical trials for the treatment of the disease. This double-blind, randomized, placebo-controlled study (phase III clinical trial) was conducted to evaluate the effectiveness of a single oral dose of ivermectin (0.15 mg/kg) in the reduction of the Mansonella ozzardi microfilaraemia and the occurrence of adverse effects in infected people compared with the control group treated with placebo. A total of 49 microfilaraemic patients were randomly selected from the municipality of Lábrea, State of Amazonas, in the Brazilian Amazon. Among them, 40 patients have concluded the study, 19 treated with ivermectin and 21 treated with placebo. In the first and third days after the treatment, all the patients were clinically evaluated, and the diagnostic and quantification of blood microfilariae through blood filtration in polycarbonate membranes was performed. A significant reduction of the microfilaraemia (99.9%) was o...
Revista da Sociedade Brasileira de Medicina Tropical
Introduction: This study reports, for the first time, the presence of Panstrongylus lignarius in ... more Introduction: This study reports, for the first time, the presence of Panstrongylus lignarius in the State of Rondonia, Brazil. Methods: Specimen of P. lignarius was collected from a flat in an urban area of the municipality of Porto Velho, Western Amazon. Results: With this finding of P. lignarius, the number of Triatominae species in the State of Rondonia has increased from six to seven. Conclusions: The occurrence of P. lignarius is worrisome, because this species has been found to be naturally infected with Trypanosoma cruzi and there has been evidence of its domiciliation capabilities in other countries of South America.
Revista da Sociedade Brasileira de Medicina Tropical, 2011
INTRODUCTION: Mansonelliasis is caused by Mansonella ozzardi. It is widespread in the Amazon regi... more INTRODUCTION: Mansonelliasis is caused by Mansonella ozzardi. It is widespread in the Amazon region, with a high prevalence. The common exam of thick blood smears stained with Giemsa shows low efficacy levels and has been an obstacle to diagnosing individuals with low blood parasitemia. METHODS: In order to increase diagnosis efficacy, the PCR technique was improved. RESULTS AND CONCLUSIONS: PCR demonstrated the best performance, with sensitivity and negative predictive values (NPV) of 100%, followed by blood filtration through membrane filters, which showed a sensitivity of 88.9% and a NPV of 84.6%, when compared to thick blood smears.
The Journal of Immunology, 2010
In many hemolytic disorders, such as malaria, the release of free heme has been involved in the t... more In many hemolytic disorders, such as malaria, the release of free heme has been involved in the triggering of oxidative stress and tissue damage. Patients presenting with severe forms of malaria commonly have impaired regulatory responses. Although intriguing, there is scarce data about the involvement of heme on the regulation of immune responses. In this study, we investigated the relation of free heme and the suppression of anti-inflammatory mediators such as PGE 2 and TGF-b in human vivax malaria. Patients with severe disease presented higher hemolysis and higher plasma concentrations of Cu/Zn superoxide dismutase (SOD-1) and lower concentrations of PGE 2 and TGF-b than those with mild disease. In addition, there was a positive correlation between SOD-1 concentrations and plasma levels of TNF-a. During antimalaria treatment, the concentrations of plasma SOD-1 reduced whereas PGE 2 and TGF-b increased in the individuals severely ill. Using an in vitro model with human mononuclear cells, we demonstrated that the heme effect on the impairment of the production of PGE 2 and TGF-b partially involves heme binding to CD14 and depends on the production of SOD-1. Aside from furthering the current knowledge about the pathogenesis of vivax malaria, the present results may represent a general mechanism for hemolytic diseases and could be useful for future studies of therapeutic approaches.
Clinics, 2005
PURPOSE: This study was carried out in Monte Negro (state of Rondônia), a village in the Brazilia... more PURPOSE: This study was carried out in Monte Negro (state of Rondônia), a village in the Brazilian western Amazon region, where a University of São Paulo Medical School program for medical student training in rural assistance took place. It aimed to determine the ...
Memorias do Instituto Oswaldo Cruz, Jul 1, 2017
The sandfly Trichophoromyia auraensis has recently evolved as a proven vector of Leishmania (Vian... more The sandfly Trichophoromyia auraensis has recently evolved as a proven vector of Leishmania (Viannia) endemic to state of Acre in the north of Brazil. This note is intended to propose a correction in the report of the first occurrence of natural infection of Leishmania (Viannia) in this species. We and the other scientific groups reinforced that Tr. auraensis is a possible vector involved in the transmission of American cutaneous leishmaniasis in Acre, Brazil.
Brazilian Journal of Otorhinolaryngology, 2015
Introduction: Mucosal leishmaniosis (ML) is a severe clinical form of leishmaniosis. Complex fact... more Introduction: Mucosal leishmaniosis (ML) is a severe clinical form of leishmaniosis. Complex factors related to the parasite and the host are attributed to the development of mucosal lesions. Leishmania RNA virus 1 (LRV1) can disrupt immune response, and may be the main determinant of severity of the disease; it should be investigated. Objective: To study the existence of clinical differences between patients with ML with endosymbiosis by LRV1 and. those without it. Methods: A cross-sectional cohort study with clinical evaluation, polymerase chain reaction (PCR) detection of Leishmania, species classification, and search of LRV1 was performed. Only patients with confirmed diagnosis of ML by positive PCR and with nasal mucosa injuries were included in this analysis. Results: Out of 37 patients, 30 (81.1%) were diagnosed with Leishmania braziliensis, five (13.5%) with Leishmania guyanensis, and two (5.4%) with mixed infection of L. braziliensis and L. guyanensis. LVR1 virus was present in 26 (70.3%) of the cases.
Cadernos de Saúde Pública, 2015
Este estudo objetivou identificar fatores associados à incidência de concessão de benefícios entr... more Este estudo objetivou identificar fatores associados à incidência de concessão de benefícios entre trabalhadores na atividade de carne e pescado no Brasil. Foram estudados os benefícios auxílio-doença concedidos pela Previdência Social aos empregados em carne e pescado no Brasil em 2008. As incidências por 10 mil vínculos empregatícios foram estratificadas por sexo, idade, diagnóstico, atividade econômica, unidade federada, espécie e duração dos benefícios auxílio-doença. Foram concedidos 31.913 benefícios auxílio-doença, com incidência de 788,7. O ramo abate de bovinos, equinos, ovinos e caprinos apresentou a maior incidência (1.223,2); o pescado, a maior duração (61 dias) de benefícios auxílio-doença. As mulheres apresentaram maior incidência, enquanto os homens, maior duração de benefícios auxílio-doença. Os grupos Lesões, doenças osteomusculares e transtornos mentais representaram 67,2% dos benefícios auxílio-doença. Os diagnósticos mais incidentes foram as dorsopatias, hemorrag...
Revista Brasileira de Epidemiologia, 2004
A Leishmaniose Tegumentar Americana (LTA) é uma doença causada por protozoários do gênero Leishma... more A Leishmaniose Tegumentar Americana (LTA) é uma doença causada por protozoários do gênero Leishmania, transmitida ao homem pela picada de mosquitos flebotomíneos (Ordem Diptera; Família Psychodidae; Sub-Família Phlebotominae). No Brasil existem atualmente 6 espécies de Leishmania responsáveis pela doença humana, e mais de 200 espécies de flebotomíneos implicados em sua transmissão. Trata-se de uma doença que acompanha o homem desde tempos remotos e que tem apresentado, nos últimos 20 anos, um aumento do número de casos e ampliação de sua ocorrência geográfica, sendo encontrada atualmente em todos os Estados brasileiros, sob diferentes perfis epidemiológicos. Estima-se que, entre 1985 e 2003, ocorreram 523.975 casos autóctones, a sua maior parte nas regiões Nordeste e Norte do Brasil. Neste estudo, são discutidos aspectos relacionados ao tratamento e ao controle dessa doença, assim como também as dificuldades para a implementação dessas medidas. São apontadas alternativas que passam ...
Saúde (Santa Maria), 2012
A presente revisão objetivou descrever um histórico da busca de fármacos para tratamento da Tripa... more A presente revisão objetivou descrever um histórico da busca de fármacos para tratamento da Tripanossomíase Americana, e sua situação atual. A pesquisa foi desenvolvida através de revisão bibliográfica do tipo exploratória, retrospectiva e descritiva baseada em livros e pesquisa de artigos em base de dados Scientific Electronic Library Online (Scielo), Biblioteca Virtual em Saúde (BVS) e National Institute of Health (PUBMED), utilizando os descritores: Tripanossomíase Americana, tratamento e perspectivas. Já se passaram mais de 100 anos da descoberta da doença de Chagas, e até hoje não foi desenvolvido nenhum fármaco com potencial satisfatório de cura. O medicamento ainda utilizado é o benzonidazol, utilizado em combinação com outros medicamentos. Entre os quimioterápicos o Posaconazol, é o mais forte candidato para novos tratamentos específicos da doença e, entre os fitoterápicos, citamse as espécies Banisteriopsis caapi e Psychotria viridis, utilizadas na região amazônica como alternativa.
Memórias do Instituto Oswaldo Cruz, 1993
Zootaxa
The larval stage of Amblyomma oblongoguttatum Koch is redescribed using optical and scanning elec... more The larval stage of Amblyomma oblongoguttatum Koch is redescribed using optical and scanning electron microscopy.Unfed larvae were obtained from a colony of A. oblongoguttatum originated from engorged females collected on domesticpigs from Monte Negro municipally (10°29’S, 63°32’W), State of Rondônia, Western Amazon, Brazil. Several charactersare presented including the chaetotaxy of the idiosoma, palpi and Haller’s organ, as well as morphological features of theidiosoma, gnathosoma and legs. In addition, the porotaxy (topographical and numerical patterns of integumentary struc-tures) were presented by using a new nomenclature recently proposed. The chaetotaxy of the larvae of A. oblongoguttat-um, in general, is similar to other Neotropical Amblyomma species. Three types of integumentary structures were observedon the idiosoma: lyrifissures, small glands, and large wax glands. Topographic and numerical patterns of the integumen-tary structures consisted of 5 pairs of large wax gland...
Ticks and Tick-borne Diseases, 2010
In the present study, we compared 2 methods for collecting ixodid ticks on the verges of animal t... more In the present study, we compared 2 methods for collecting ixodid ticks on the verges of animal trails in a primary Amazon forest area in northern Brazil. (i) Dragging: This method was based on passing a 1-m2 white flannel over the vegetation and checking the flannel for the presence of caught ticks every 5–10m. (ii) Visual search: This method consisted
Arquivos do Instituto Biológico
RESUMO O presente estudo determinou a prevalência de anticorpos anti-Leptospira spp. em ovinos do... more RESUMO O presente estudo determinou a prevalência de anticorpos anti-Leptospira spp. em ovinos do Município de Monte Negro, RO. Foram examinados soros de 141 ovinos de raça, idade e sexo variados provenientes de 15 fazendas, pela técnica de Soroaglutinação Microscópica. Doze (80,0%) propriedades apresentaram pelo menos um animal reagente. Títulos de anticorpos iguais ou superiores a 100 foram detectados em 47 (33,3%) animais, e os sorovares mais frequentes foram Patoc (29,7%), Autumnalis (14,8%), Pyrogenes (10,6%), Australis (4,2%), Bratislava (4,2%), Hardjo (4,2%), Icterohaemorrhagiae (4,2%), Castellonis (2,1%) e Hebdomadis (2,1%). Em 11 (23,4%) soros não foi possível a determinação do provável sorovar envolvido na reação. Alerta-se também para a possibilidade de infecção no homem, tendo em vista as características regionais de fronteira agrícola amazônica.
Zootaxa
We present here a multisource approach that takes advantage of several disciplines to address a t... more We present here a multisource approach that takes advantage of several disciplines to address a taxonomic issue. A triatomine related to Rhodnius robustus Larrousse, 1927 was recently found in the state of Rondônia, Brazil. The name Rhodnius montenegrensis n. sp. is suggested because it was found in the municipality of Monte Negro. The main differences between these two species can be detected in the female and male genitalia, but there are also noticeable differences in their eggs. Molecular analysis using PCR-RFLP technique and Bayesian inferences based on a fragment of the Cytochrome b (Cyt b) gene corroborated the morphological findings. We used this integrative approach to address the taxonomic decision for a new Rhodnius species and its relationship with others of this genus. Results obtained herein stress that morphology must be used as the major approach for obtaining phenotypic information, and molecular data should be taken as a complementary tool.
Vector borne and zoonotic diseases (Larchmont, N.Y.), Nov 17, 2018
Rhodnius montenegrensis was described in 2012. Since then, reports of the occurrence of this spec... more Rhodnius montenegrensis was described in 2012. Since then, reports of the occurrence of this species associated with palm trees near households in Rondônia have been published. This study aimed to analyze the natural infection of R. montenegrensis with trypanosomatids in the municipality of Monte Negro, Rondônia, Brazilian western Amazon. Capture of triatomines occurred in Attalea speciosa (babassu) specimens around households. Twelve of the 72 captured triatomines were identified by morphological and morphometric characters, by molecular analysis made using the PCR, DNA sequencing, and phylogenetic analyses. The intestinal content was collected from 60 of these 72 specimens. The positivity for trypanosomatids was confirmed by examining the intestinal content followed by PCR amplification of the cathepsin L-like gene specific for Trypanosoma cruzi (PCR-DTcrCatL) and Trypanosoma rangeli (PCR-DTraCatL). Of the 60 specimens analyzed by microscopy, 22 (36.7%) were positive for trypanoso...
Revista da Sociedade Brasileira de Medicina Tropical
This communication reports the occurrence of Rhodnius neglectus in the State of Acre, Brazil. An ... more This communication reports the occurrence of Rhodnius neglectus in the State of Acre, Brazil. An adult male R. neglectus specimen was collected from the Catuaba Experimental Reserve, which is located in the municipality of Senador Guiomard. This increases the number of triatomine species reported from the State of Acre to eight and is also the first report of R. neglectus from the Brazilian Western Amazon Region. The occurrence of R. neglectus is alarming because even though the species is wild, it can invade and colonize human dwellings and peridomiciles, thereby increasing Chagas disease transmission.
Revista da Sociedade Brasileira de Medicina Tropical
The occurrence of Triatoma sordida in the Brazilian Western Amazon is reported for the first time... more The occurrence of Triatoma sordida in the Brazilian Western Amazon is reported for the first time. Triatoma sordida specimens were collected from a Gallus gallus nest in a peridomiciliary area of Senador Guiomard municipality in the state of Acre. The number of triatomine species in Acre increased from six to seven with this first report of T. sordida in the Brazilian Western Amazon. The occurrence of T. sordida in Acre is of concern since it is among the most captured triatomines in peridomiciliary environments in Brazil, and carries a high potential for vector transmission.
Arquivos brasileiros de oftalmologia
Malignant lesions of the eyelid are the most common eye cancers. Although rarely lethal, late dia... more Malignant lesions of the eyelid are the most common eye cancers. Although rarely lethal, late diagnosis can lead to serious visual morbidity. Definitive diagnosis of neoplastic lesions is pathological. Indications and types of eyelid lesions in a single high-volume ophthalmic reference center in Sao Paulo, Brazil, were analyzed. The surgical pathological reports of patients who underwent eye removal procedures between January 2000 and December 2012 were retrieved from the electronic database of the Ophthalmology Department and retrospectively reviewed. Data regarding the final anatomopathological diagnosis, sex, and age were analyzed via the χ2 test with Yates' correction. Of the 1,113 eyelid tumors resected over the 13-year study period, 324 (29%) lesions were malignant. The most prevalent lesions were basal cell, squamous cell, and sebaceous gland carcinomas. The median ages of patients (females, n=165, 51%; males, n=159, 49%) with a diagnosis of basal cell, squamous cell, and...
Revista da Sociedade Brasileira de Medicina Tropical
Rhodnius stali infection by Trypanosoma rangeli is reported in this study for the first time. The... more Rhodnius stali infection by Trypanosoma rangeli is reported in this study for the first time. The triatomines were collected from the campus of the Federal University of Acre in Rio Branco, Acre, Brazil. The identification of T. rangeli was confirmed by multiplex polymerase chain reaction. The examinations of two specimens revealed R. stali infection by the epimastigote forms of T. rangeli. The encounter of R. stali infected by T. rangeli generates an alert for the state of Acre, since the simultaneous presence with Trypanosoma cruzi can make the differential diagnosis of Chagas disease difficult.
The American journal of tropical medicine and hygiene, Jan 8, 2018
The treatment of mansonelliasis is still a challenge because there are few clinical trials for th... more The treatment of mansonelliasis is still a challenge because there are few clinical trials for the treatment of the disease. This double-blind, randomized, placebo-controlled study (phase III clinical trial) was conducted to evaluate the effectiveness of a single oral dose of ivermectin (0.15 mg/kg) in the reduction of the Mansonella ozzardi microfilaraemia and the occurrence of adverse effects in infected people compared with the control group treated with placebo. A total of 49 microfilaraemic patients were randomly selected from the municipality of Lábrea, State of Amazonas, in the Brazilian Amazon. Among them, 40 patients have concluded the study, 19 treated with ivermectin and 21 treated with placebo. In the first and third days after the treatment, all the patients were clinically evaluated, and the diagnostic and quantification of blood microfilariae through blood filtration in polycarbonate membranes was performed. A significant reduction of the microfilaraemia (99.9%) was o...
Revista da Sociedade Brasileira de Medicina Tropical
Introduction: This study reports, for the first time, the presence of Panstrongylus lignarius in ... more Introduction: This study reports, for the first time, the presence of Panstrongylus lignarius in the State of Rondonia, Brazil. Methods: Specimen of P. lignarius was collected from a flat in an urban area of the municipality of Porto Velho, Western Amazon. Results: With this finding of P. lignarius, the number of Triatominae species in the State of Rondonia has increased from six to seven. Conclusions: The occurrence of P. lignarius is worrisome, because this species has been found to be naturally infected with Trypanosoma cruzi and there has been evidence of its domiciliation capabilities in other countries of South America.