Luis García - Academia.edu (original) (raw)
Papers by Luis García
Process Biochemistry, 2003
... Carlos de la Roza a , Adriana Laca a , Luis A. Garcı́a a and Mario Dı́az Corresponding Author... more ... Carlos de la Roza a , Adriana Laca a , Luis A. Garcı́a a and Mario Dı́az Corresponding Author Contact Information , a , E-mail The ... In this sense, beer is probably the beverage whose production process has been most studied and several models have even been proposed to ...
Journal of Pharmaceutical and Biomedical Analysis, 1999
Journal of Comparative Pathology, 2003
The finding of brain tissue fragments in blood and lungs of cattle stunned in slaughterhouses has... more The finding of brain tissue fragments in blood and lungs of cattle stunned in slaughterhouses has raised concerns about food safety in the context of the bovine spongiform encephalopathy epidemic. In the present study, the possible occurrence of brain tissue emboli in animals killed in traditional Spanish bullfighting was investigated. Thorough histological analysis of multiple possible target organs was carried out in 434 bulls. No evidence of brain tissue embolism was obtained, but emboli from diverse sources were detected in pulmonary and hepatic tissue of a significant number of animals. These emboli seem to have been caused by the use of a long sword, which extensively disrupts intra-thoracic and intra-abdominal organs and vascular structures.
La esquizofrenia es un síndrome psicopatológico variable, crónicoy por lo general deteriorante, q... more La esquizofrenia es un síndrome psicopatológico variable, crónicoy por lo general deteriorante, que compromete la percepción, elpensamiento, las emociones, el movimiento y el comportamiento. Su etiología no es clara, pero en algunos pacientes parece deberse a la herencia de variantes génicas que interfieren en el neurodesarrollo. Esta hipótesis está fundamentada principalmente en las alteraciones neuroanatómicas y funcionales obtenidas por imaginología y en los estudios postmortem, lo cual sugiere que un inadecuado neurodesarrollo origina un cerebro con estructura y funcionamiento anormales ue lo hacen más vulnerable a efectos adversos del ambiente interno y externo del individuo y lo predisponen al desarrollo de la enfermedad (1). Bajo este enfoque, uno de los genes involucrados en el fenotipo esquizofrenia es RELN, el cual está ubicado en la región 7q22 y codifica una glicoproteína llamada relina implicada en el posicionamiento y migración de neuronas al hipocampo y la corteza dur...
Journal of Biotechnology, 2000
Different immobilised cells models, including very simple ones, can be useful in the fitting of e... more Different immobilised cells models, including very simple ones, can be useful in the fitting of experimental results. However, goodness or the ability to extrapolate results needs to be in accordance with basic observations and these will also suggest models to be proposed. In this paper, observations of calcium alginate/bacteria systems are used to show the ability of basic models to fit classic observations, as well as how new observations, in this case from electronic microscopy, oblige us to think about more complex mechanisms and mathematical treatments. Nevertheless it is not only important to discuss the model type, but also the type of kinetics assumed in the interior of the beads, as well as the internal structure, the boundary conditions related to bead shredding and cell escape and finally, geometrical effects.
Experimental Cell Research, 1996
Protease nexin I (PNI) is a 43- to 50-kDa glycoprotein capable of inhibiting a number of serine p... more Protease nexin I (PNI) is a 43- to 50-kDa glycoprotein capable of inhibiting a number of serine proteases and belongs to the serpin superfamily. PNI is identical to glia-derived nexin, a neurite outgrowth promoter by virtue of its thrombin-inhibiting activity. Of particular relevance to neuromuscular biology and pathology, PNI was the first serpin shown to be highly localized to the neuromuscular junction and it maps to precisely the same locus as autosomal recessive amyotrophic lateral sclerosis (ALSJ) at chromosome 2q33-35. In the present report, we now show that in cultures of human skeletal muscle, PNI protein is expressed only after myoblast fusion into multinuclear myotubes and is localized in patches on their surfaces. We performed complex formation experiments with labeled thrombin, another target protease for PNI, with intact human muscle cells in culture. We detected specific SDS-stable PNI/thrombin complexes in myotube extracts only, indicating that active PNI was bound to their surfaces. We studied the gene expression of PNI mRNA using a 300-bp cDNA synthesized from the published sequence of human PNI. Confirming the protein data, upregulation of PNI appears in myotubes using Northern blot analysis. The current results reinforce the hypothesis that the regulation of the balance of serine proteases and serpins, such as PNI, is involved in muscle differentiation. They also prompt us to explore PNI abnormalities in several neuromuscular diseases, including ALSJ.
Enzyme and Microbial Technology, 2001
Genetics and Molecular Biology, 2015
A significant association between HFE gene mutations and the HLA-A*03-B*07 and HLA-A*29-B*44 hapl... more A significant association between HFE gene mutations and the HLA-A*03-B*07 and HLA-A*29-B*44 haplotypes has been reported in the Spanish population. It has been proposed that these mutations are probably connected with Celtic and North African ancestry, respectively. We aimed to find the possible ancestral association between HLA alleles and haplotypes associated with the HFE gene (C282Y and H63D) mutations in 214 subjects from Antioquia, Colombia. These were 18 individuals with presumed hereditary hemochromatosis…
Environmental Health Perspectives, 2007
Gayana (Concepción), 2009
ABSTRACT Se registra Anelosimus lorenzo para Uruguay, constituyendo el hallazgo más austral de la... more ABSTRACT Se registra Anelosimus lorenzo para Uruguay, constituyendo el hallazgo más austral de la especie. El presente registro confirma la inusual presencia de una araña social para una zona de clima templado. Con este nuevo reporte, el número de especies de Anelosimus conocidas para este país se eleva a tres.
Journal of Arachnology, 2014
Biosystems Engineering, 2004
Medicina, 2007
The immune response elicited by an allogenic transplant usually leads to an effector response res... more The immune response elicited by an allogenic transplant usually leads to an effector response resulting in allograft rejection; however, some individuals maintain a long-term functioning transplant without signs of rejection (operational tolerance) even in the absence of immunosuppression. It has been suggested that the same mechanisms are responsible for tolerance to self-antigens and alloantigens. One of such mechanisms is immune regulation and several cell subsets with regulatory properties have been identified. Among them, the best characterized cell populations are the regulatory T cells (Treg). Although Treg in mice are CD4+CD25+, in humans the Treg phenotype is restricted to CD4 T cells with high expression of CD25 (CD25high) and Foxp3. Phenotypic and functional analysis of circulating regulatory or suppressor T cells in transplant patients may be useful for detection of operationally tolerant patients. Moreover, future in vitro manipulation of these cells with therapeutic pu...
Biochemical Engineering Journal, 2004
Biomédica : revista del Instituto Nacional de Salud, 2007
Genetic characterization of the human leucocyte antigen (HLA) system has provided insights into m... more Genetic characterization of the human leucocyte antigen (HLA) system has provided insights into mechanisms of susceptibility to diverse diseases and immunological phenomena during pregnancy, as well as providing evidence for compatibility in the selection of organ transplant donors and recipients. The HLA-A,-B,-DRB1 allele, genotype and haplotype frequencies were determined in deceased organ donors in Medellín, Colombia. The genotypes of 926 deceased donors were evaluated over a 17-year period (1989- 2006). HLA-A, HLA-B and HLA-DRB1 typing was performed by sequence specific primer-polymerase chain reaction (SSP-PCR). Maximum likelihood frequencies were estimated by the zipper version of expectation maximation algorithm. Hardy-Weinberg equilibrium were determined by an exact test analogous to Fishers test by using Markovs chain, and linkage disequilibrium between pairs of loci. Twenty-two, 43 and 14 alleles were identified for HLA-A, -B and -DRB loci, respectively. The most frequent ...
Process Biochemistry, 2003
... Carlos de la Roza a , Adriana Laca a , Luis A. Garcı́a a and Mario Dı́az Corresponding Author... more ... Carlos de la Roza a , Adriana Laca a , Luis A. Garcı́a a and Mario Dı́az Corresponding Author Contact Information , a , E-mail The ... In this sense, beer is probably the beverage whose production process has been most studied and several models have even been proposed to ...
Journal of Pharmaceutical and Biomedical Analysis, 1999
Journal of Comparative Pathology, 2003
The finding of brain tissue fragments in blood and lungs of cattle stunned in slaughterhouses has... more The finding of brain tissue fragments in blood and lungs of cattle stunned in slaughterhouses has raised concerns about food safety in the context of the bovine spongiform encephalopathy epidemic. In the present study, the possible occurrence of brain tissue emboli in animals killed in traditional Spanish bullfighting was investigated. Thorough histological analysis of multiple possible target organs was carried out in 434 bulls. No evidence of brain tissue embolism was obtained, but emboli from diverse sources were detected in pulmonary and hepatic tissue of a significant number of animals. These emboli seem to have been caused by the use of a long sword, which extensively disrupts intra-thoracic and intra-abdominal organs and vascular structures.
La esquizofrenia es un síndrome psicopatológico variable, crónicoy por lo general deteriorante, q... more La esquizofrenia es un síndrome psicopatológico variable, crónicoy por lo general deteriorante, que compromete la percepción, elpensamiento, las emociones, el movimiento y el comportamiento. Su etiología no es clara, pero en algunos pacientes parece deberse a la herencia de variantes génicas que interfieren en el neurodesarrollo. Esta hipótesis está fundamentada principalmente en las alteraciones neuroanatómicas y funcionales obtenidas por imaginología y en los estudios postmortem, lo cual sugiere que un inadecuado neurodesarrollo origina un cerebro con estructura y funcionamiento anormales ue lo hacen más vulnerable a efectos adversos del ambiente interno y externo del individuo y lo predisponen al desarrollo de la enfermedad (1). Bajo este enfoque, uno de los genes involucrados en el fenotipo esquizofrenia es RELN, el cual está ubicado en la región 7q22 y codifica una glicoproteína llamada relina implicada en el posicionamiento y migración de neuronas al hipocampo y la corteza dur...
Journal of Biotechnology, 2000
Different immobilised cells models, including very simple ones, can be useful in the fitting of e... more Different immobilised cells models, including very simple ones, can be useful in the fitting of experimental results. However, goodness or the ability to extrapolate results needs to be in accordance with basic observations and these will also suggest models to be proposed. In this paper, observations of calcium alginate/bacteria systems are used to show the ability of basic models to fit classic observations, as well as how new observations, in this case from electronic microscopy, oblige us to think about more complex mechanisms and mathematical treatments. Nevertheless it is not only important to discuss the model type, but also the type of kinetics assumed in the interior of the beads, as well as the internal structure, the boundary conditions related to bead shredding and cell escape and finally, geometrical effects.
Experimental Cell Research, 1996
Protease nexin I (PNI) is a 43- to 50-kDa glycoprotein capable of inhibiting a number of serine p... more Protease nexin I (PNI) is a 43- to 50-kDa glycoprotein capable of inhibiting a number of serine proteases and belongs to the serpin superfamily. PNI is identical to glia-derived nexin, a neurite outgrowth promoter by virtue of its thrombin-inhibiting activity. Of particular relevance to neuromuscular biology and pathology, PNI was the first serpin shown to be highly localized to the neuromuscular junction and it maps to precisely the same locus as autosomal recessive amyotrophic lateral sclerosis (ALSJ) at chromosome 2q33-35. In the present report, we now show that in cultures of human skeletal muscle, PNI protein is expressed only after myoblast fusion into multinuclear myotubes and is localized in patches on their surfaces. We performed complex formation experiments with labeled thrombin, another target protease for PNI, with intact human muscle cells in culture. We detected specific SDS-stable PNI/thrombin complexes in myotube extracts only, indicating that active PNI was bound to their surfaces. We studied the gene expression of PNI mRNA using a 300-bp cDNA synthesized from the published sequence of human PNI. Confirming the protein data, upregulation of PNI appears in myotubes using Northern blot analysis. The current results reinforce the hypothesis that the regulation of the balance of serine proteases and serpins, such as PNI, is involved in muscle differentiation. They also prompt us to explore PNI abnormalities in several neuromuscular diseases, including ALSJ.
Enzyme and Microbial Technology, 2001
Genetics and Molecular Biology, 2015
A significant association between HFE gene mutations and the HLA-A*03-B*07 and HLA-A*29-B*44 hapl... more A significant association between HFE gene mutations and the HLA-A*03-B*07 and HLA-A*29-B*44 haplotypes has been reported in the Spanish population. It has been proposed that these mutations are probably connected with Celtic and North African ancestry, respectively. We aimed to find the possible ancestral association between HLA alleles and haplotypes associated with the HFE gene (C282Y and H63D) mutations in 214 subjects from Antioquia, Colombia. These were 18 individuals with presumed hereditary hemochromatosis…
Environmental Health Perspectives, 2007
Gayana (Concepción), 2009
ABSTRACT Se registra Anelosimus lorenzo para Uruguay, constituyendo el hallazgo más austral de la... more ABSTRACT Se registra Anelosimus lorenzo para Uruguay, constituyendo el hallazgo más austral de la especie. El presente registro confirma la inusual presencia de una araña social para una zona de clima templado. Con este nuevo reporte, el número de especies de Anelosimus conocidas para este país se eleva a tres.
Journal of Arachnology, 2014
Biosystems Engineering, 2004
Medicina, 2007
The immune response elicited by an allogenic transplant usually leads to an effector response res... more The immune response elicited by an allogenic transplant usually leads to an effector response resulting in allograft rejection; however, some individuals maintain a long-term functioning transplant without signs of rejection (operational tolerance) even in the absence of immunosuppression. It has been suggested that the same mechanisms are responsible for tolerance to self-antigens and alloantigens. One of such mechanisms is immune regulation and several cell subsets with regulatory properties have been identified. Among them, the best characterized cell populations are the regulatory T cells (Treg). Although Treg in mice are CD4+CD25+, in humans the Treg phenotype is restricted to CD4 T cells with high expression of CD25 (CD25high) and Foxp3. Phenotypic and functional analysis of circulating regulatory or suppressor T cells in transplant patients may be useful for detection of operationally tolerant patients. Moreover, future in vitro manipulation of these cells with therapeutic pu...
Biochemical Engineering Journal, 2004
Biomédica : revista del Instituto Nacional de Salud, 2007
Genetic characterization of the human leucocyte antigen (HLA) system has provided insights into m... more Genetic characterization of the human leucocyte antigen (HLA) system has provided insights into mechanisms of susceptibility to diverse diseases and immunological phenomena during pregnancy, as well as providing evidence for compatibility in the selection of organ transplant donors and recipients. The HLA-A,-B,-DRB1 allele, genotype and haplotype frequencies were determined in deceased organ donors in Medellín, Colombia. The genotypes of 926 deceased donors were evaluated over a 17-year period (1989- 2006). HLA-A, HLA-B and HLA-DRB1 typing was performed by sequence specific primer-polymerase chain reaction (SSP-PCR). Maximum likelihood frequencies were estimated by the zipper version of expectation maximation algorithm. Hardy-Weinberg equilibrium were determined by an exact test analogous to Fishers test by using Markovs chain, and linkage disequilibrium between pairs of loci. Twenty-two, 43 and 14 alleles were identified for HLA-A, -B and -DRB loci, respectively. The most frequent ...