Luis carlos villamil - Academia.edu (original) (raw)

Papers by Luis carlos villamil

Con el objeto de conocer el metodo mas eficiente para el examen morfologico de Trichomonas, se en... more Con el objeto de conocer el metodo mas eficiente para el examen morfologico de Trichomonas, se ensayaron 5 tipos de coloracion de Giemsa empleando 100 extensiones de cultivos positivos para cada coloracion. Los metodos se basaron en fijacion con acetona-alcohol, cloruro mercurico, etanol, acido acetico y metanol y la coloracion con Giemsa o el metodo combinado May-Gruenwald Giemsa. La eficiencia de los diferentes metodos se evaluo teniendo en cuenta la visualizacion de los siguientes elementos: flagelos anteriores, membrana ondulante, axostilo y cuerpo parabasal. El metodo mas satisfactorio se obtuvo haciendo la fijacion con metanol durante 15-30 minutos y coloracion con Giemsa. La tecnica fue valorada nuevamente comparandola en 246 extensiones de lavados prepuciales frente al examen microscopico correspondiente a las mismas muestras. Esta coloracion permitio identificar mejor las estructuras de la T. foetus y por lo tanto se recomienda su utilizacion como metodo auxiliar para el diagnostico de la trichomoniasis genital bovina

Revista de la Facultad de Medicina Veterinaria y de Zootecnia, 1996

Revista de la Facultad de Medicina Veterinaria y de Zootecnia, 1994

Se analizaron aislamientos de campo y de laboratorio de las cepas citopaticas (CP) y no citopatic... more Se analizaron aislamientos de campo y de laboratorio de las cepas citopaticas (CP) y no citopaticas (NCP) nacionales y de referencia del virus de la diarrea viral bovina (VDVB) [...]

Revista De Salud Publica, Oct 8, 2010

involucrando aspectos históricos de su constitución y desarrollo, los campos de acción y los reto... more involucrando aspectos históricos de su constitución y desarrollo, los campos de acción y los retos actuales. Igualmente presenta una reflexión de la SPV en el marco de la reestructuración de los servicios veterinarios y finalmente plantea como la educación juega un papel determinante para poder enfrentar los retos de una nueva época.

Revista Lasallista De Investigacion, Jul 1, 2010

Proyecto académico sin fines de lucro, desarrollado bajo la iniciativa de acceso abierto

A Longitudinal epidemiologic study was developed for the isolation, biochemical characterization ... more A Longitudinal epidemiologic study was developed for the isolation, biochemical characterization and molecular typing the bacterial pathogen Clostridium spp found in soil from areas affected by bovine sudden mortality. Weincluded 10 herds, located in three localities. The genetic variability of Clostridium genus was analyzed by DNA sequence of a 1500 bp fragment from the 16S rRNA gene. Twenty four Clostridium isolates were biochemically classified as Clostridium sordellii (41,7%), Clostridium glycolicum (12,5%), Clostridium hastiforme (12,5%); Clostridium botulinum (8,3%), Clostridium butyricum (8,3%); Clostridium chauvoei (4,3%),Clostridium limosum (4,3%), Clostridium septicum (4,3%) and Clostridium tertium (4,3%). The bacteria that showed pathogenic activity were studied further by 16S rRNA gene sequencing at, which 55.5% was classified as C. botulinum, where the native strain isolated from areas with outbreaks wasfound in a different group from Clostridium spp. used by commercial laboratories for vaccine production. Additionally, the native strain identified here differs from others reported in Gen Bank, indicating that the native pathogenic Clostridium spp. is genetically different to other Clostridium spp. used to prepare immunogens affecting vaccine efficiency. Our results indicate that the use of native strains could improve commercial vaccine preparations, increasing bovine immune response.

Rev. salud pública, 2002

The role of veterinary public health in the demand of services for colombian cattle production Th... more The role of veterinary public health in the demand of services for colombian cattle production This study applies Stakeholder Analysis to appraise the role of veterinary public health within service demand for cattle production in Colombia after the process of restructuring of veterinary services. An exploratory study of the livestock service sector was carried out over 13 months through individual open-ended interviews at regional and national levels. The study involved five main stakeholders: producers, service providers, government, training institutions and product purchasers. Four regional case studies examined smallholder, dairy, beef and dual purpose cattle production systems. Despite service availability and professional oversupply , low demand for services and unsatisfied needs were detected. Veterinary public health was widely omitted, highlighting the need for the active role that the public sector must have in policy-making for provision of these services. Some policy recommendations which involve all stakeholders are presented. Despite limitations, the potential and value of participative approaches as a complementary tool is emphasized.

Revista de Salud Pública, 1999

Revista de Medicina Veterinaria, 2011

Revista de Medicina Veterinaria, 2022

This Editorial is brought to you for free and open access by the Revistas científicas at Ciencia ... more This Editorial is brought to you for free and open access by the Revistas científicas at Ciencia Unisalle. It has been accepted for inclusion in Revista de Medicina Veterinaria by an authorized editor of Ciencia Unisalle. For more information, please contact

Revue Scientifique et Technique de l'OIE, 1999

The authors discuss the relative importance of the livestock sector in South America, in developi... more The authors discuss the relative importance of the livestock sector in South America, in developing countries and in countries world-wide. The development of animal health services is highlighted and the new challenges which these services must meet, with respect to the restructuring of international trade, are described. The economic impact of animal diseases and of disease control is evaluated by taking a regional approach and by analysing production systems. A classification of production systems is accompanied by case studies on the economics of animal health. Emphasis is placed on national control programmes, diseases which affect reproduction, parasitic diseases and mastitis, with particular reference to the livestock sector of Colombia. The authors suggest that integrated livestock development programmes include the management of animal health and production information. A change of attitude is also recommended with greater participation of those involved. The need to co-ordinate sustained research initiatives is stressed.

Leptospirosis es una zoonosis que impacta la salud humana, animal y ambiental; en los bovinos y p... more Leptospirosis es una zoonosis que impacta la salud humana, animal y ambiental; en los bovinos y porcinos se presentan pérdidas por problemas reproductivos que inciden negativamente en la producción. Objetivo: identificar genes de patogenicidad (lipl32 y ompl1) asociados con Leptospirosis en Cundinamarca, Colombia. Materiales y Métodos: se utilizaron 26 cepas, 10 aisladas de orina de porcinos y 16 fueron obtenidas como muestra representativa (I-C 95%) de 70 cepas aisladas de orina de bovinos en un estudio previo (Hernández-Rodríguez et al., 2011). Con el DNA extraído se amplificaron los genes asociados con patogenicidad, lipl32 y ompl1; también las secuencias rrl y rrs que identifican el género Leptospira y leptospiras saprófitas. Como control positivo se utilizó la cepa Leptospira interrogans (ATCC); y como controles negativos las cepas ATTC Leptospira biflexa (saprófita) y Escherichia coli. Resultados y Discusión: en el 100% de las muestras se identificaron amplicones de 960 pb y 4...

Infectious diseases, considered mostly of zoonotic origin, have significant impacts on wildlife, ... more Infectious diseases, considered mostly of zoonotic origin, have significant impacts on wildlife, domestic animals and humans. Anthropogenic changes, extensive use of land and agriculture, among other factors, are involved in the apparent increase in the frequency of zoonotic diseases in recent decades. As an approach to human/animal interface in Neotropical conditions, cases of zoonotic diseases of viral origin (West Nile fever) and other bacterial (mycobacteriosis), where samples were taken for detection of agents causes of these diseases, based on field methodologies (commercial tests) and laboratory (plates essays, staining and microscopic observation, microbiological culture and molecular techniques), in populations of resident and migratory wild birds in island locations (San Andres Island) and continental in Colombia, in oropharyngeal swabs, cloacal swabs, blood, internal organs, among others, from 300 birds for West Nile virus and 250 birds for mycobacteria. Additionally, hyp...

This paper underscores the need for animal health professionals to play a more significant role i... more This paper underscores the need for animal health professionals to play a more significant role in the sphere of public health, particularly during natural disasters and other emergency situations which can reduce the availability of safe foods from animal sources. In order to help readers understand the importance of the emerging field of veterinary public health (VPH), the authors review the importance and current status of VPH in different countries and assess the role that veterinarians can play in overcoming situations that threaten human health. The last section discusses the need for training veterinarians in VPH and the important role that veterinarians can play within international public health organizations and multidisciplinary groups, such as SAPUVET and SPVet networks.