Luis del Rio - Academia.edu (original) (raw)
Papers by Luis del Rio
Plant Physiol Biochem, 2002
Growth and development in all organisms occur as a result of an overall balance between synthesis... more Growth and development in all organisms occur as a result of an overall balance between synthesis and proteolysis. In plants, protein degradation is a crucial mechanism in some developmental stages such as germination, morphogenesis and cell biogenesis, senescence, and programmed cell death. In this work, the main proteases that take part in these processes are reviewed. Proteolysis is also an important component together with protein oxidation in oxidative stress situations induced by senescence and heavy metals. The presence of exo- and endoproteolytic activity in plant peroxisomes is analyzed, and the role of peroxisomal proteases in different physiological events that take place under oxidative stress situations is discussed.
... ou de Pondal. A partir de agora débese engadir a esa nómina o traballo de Aguirre del 13ío, q... more ... ou de Pondal. A partir de agora débese engadir a esa nómina o traballo de Aguirre del 13ío, que sempre se citou como per-dido e que nesta súa primeira edición ofrecemos felizmente ó publico interesado. О manuscrito de ...
Free Radical Res, 1999
The effect of growing pea plants with 50 microM CdCl2 on the activated oxygen metabolism was stud... more The effect of growing pea plants with 50 microM CdCl2 on the activated oxygen metabolism was studied at subcellular level in peroxisomes isolated from pea leaves. Cadmium treatment produced proliferation of peroxisomes as well as an increase in the content of H2O2 in peroxisomes from pea leaves, but in peroxisomal membranes no significant effect on the NADH-dependent O2*- production was observed. The rate of lipid peroxidation of membranes was slightly decreased in peroxisomes from Cd-treated plants. This could be due to the Cd-induced increase in the activity of some antioxidative enzymes involved in H2O2 removal, mainly ascorbate peroxidase and glutathione reductase, as well as the NADP-dependent dehydrogenases present in these organelles. The activity of xanthine oxidase did not experiment changes by Cd treatment and this suggests that O2*- production in the peroxisomal matrix is not involved in Cd toxicity. This was supported by the absence of changes in plants treated with Cd in the Mn-SOD activity, responsible for O2*- removal in the peroxisomal matrix. Results obtained indicate that toxic Cd levels induce imbalances in the activated oxygen metabolism of pea leaf peroxisomes, but its main effect is an enhancement of the H2O2 concentration of these organelles. Peroxisomes respond to Cd toxicity by increasing the activity of antioxidative enzymes involved in the ascorbate-glutathione cycle and the NADP-dependent dehydrogenases located in these organelles.
Free Radical Research, Feb 1, 1997
Cytosolic copperzinc-superoxide dismutase (CuZn-SOD I; EC 1.15.1.1) was purified to homogeneity f... more Cytosolic copperzinc-superoxide dismutase (CuZn-SOD I; EC 1.15.1.1) was purified to homogeneity from watermelon (Citrullus vulgaris Schrad.) cotyledons. The stepwise purification procedure consisted of acetone precipitation, batch anion-exchange chromatography, anion-exchange Fast Protein Liquid Chromatography, gel-filtration column chromatography, and affinity chromatography on concanavalin A-Sepharose. CuZn-SOD I was purified 310-fold with a yield of 12.6 micrograms enzyme per gram cotyledons, and had a specific activity of 3,450 units per milligram protein. The relative molecular mass for cytosolic CuZn-SOD was 34000, and it was composed by two equal subunits of 16.3 kDa. CuZn-SOD I did not contain neutral carbohydrates in its molecule, and its ultraviolet and visible absorption spectra showed two absorption maxima at 254 nm and 580 nm. Metal analysis showed that the enzyme contained 1 gram-atom Cu and 1 gram-atom Zn per mole dimer. Cytosolic CuZn-SOD was recognized by the antibody against peroxisomal CuZn-SOD from watermelon cotyledons, and its enzymatic activity was inhibited by this antibody. By IEF (pH 4.2-4.9), using a new method for vertical slab gels set up in our laboratory, purified cytosolic CuZn-SOD was resolved into two equal isoforms with isoelectric point of 4.63 and 4.66.
Http Dx Doi Org 10 4161 Psb 3 5 5277, May 1, 2008
In animal cells, nitric oxide and NO-derived molecules have been shown to mediate post-translatio... more In animal cells, nitric oxide and NO-derived molecules have been shown to mediate post-translational modifications such as S-nitrosylation and protein tyrosine nitration which are associated with cell signalling and pathological processes, respectively. In plant cells, knowledge of the function of these post-translational modifications under physiological and stress conditions is still very rudimentary. In this addendum, we briefly examine how reactive nitrogen species (RNS) can exert important effects on proteins that could mediate signalling processes in plants.
Plant Physiology, Aug 1, 1989
The activity and isozymic composition of superoxide dismutase (SOD; EC 1.15.1.1) were determined ... more The activity and isozymic composition of superoxide dismutase (SOD; EC 1.15.1.1) were determined in nodules of Phaseolus vulgaris L., Pisum sativum L., and Vigna ungukulata (L.) Walp. formed by Rhizoblum phaseoll 3622, R. leguminosarum 3855, and Bradyrhizobium sp. BR7301, respectively. A Mn-SOD was present in Rhizobium and two in Bradyrhizobium and bacteroids. Nodule mitochondria from all three legume species had a single Mn-SOD with similar relative mobility, whereas the cytosol contained several CuZn-SODs: two in Phaseolus and Pisum, and four in Vigna.
Horticultura Revista De Industria Distribucion Y Socioeconomia Horticola Frutas Hortalizas Flores Plantas Arboles Ornamentales Y Viveros, 2007
Free Radical Res, 1997
In previous works using cell fractionation methods we demonstrated the presence of a Cu,Zn-contai... more In previous works using cell fractionation methods we demonstrated the presence of a Cu,Zn-containing superoxide dismutase in peroxisomes from watermelon cotyledons. In this work, this intracellular localization was evaluated by using western blot and EM immunocytochemical analysis with a polyclonal antibody against peroxisomal Cu,Zn-SOD II from watermelon cotyledons. In crude extracts from 6-day old cotyledons, analysis by western blot showed two cross-reactivity bands which belonged to the isozymes Cu,Zn-SOD I and Cu,Zn-SOD II. In peroxisomes purified by sucrose density-gradient centrifugation only one cross-reactivity band was found in the peroxisomal matrix which corresponded to the isozyme Cu,Zn-SOD II. When SOD activity was assayed in purified peroxisomes two isozymes were detected, Cu,Zn-SOD II in the matrix, and a Mn-SOD in the membrane fraction which was removed by sodium carbonate washing. EM immunocytochemistry of Cu,Zn-SOD on sections of 6-day old cotyledons, showed that gold label was mainly localized over plastids and also in peroxisomes and the cytosol, whereas mitochondria did not label for Cu,Zn-SOD.
... Tal sistema debería proporcionar las siguientes ventajas: - Una plataforma de tecnología inte... more ... Tal sistema debería proporcionar las siguientes ventajas: - Una plataforma de tecnología integrada que se añadiría a las inver-siones ya realizadas por una organización, para proporcionar infor-mación de alta calidad a cada PC o servidor del departamento, aña-diendo ...
Revista Espanola De Drogodependencias, 2006
Plant and Soil, May 1, 2010
Plant Physiol Biochem, 2002
Growth and development in all organisms occur as a result of an overall balance between synthesis... more Growth and development in all organisms occur as a result of an overall balance between synthesis and proteolysis. In plants, protein degradation is a crucial mechanism in some developmental stages such as germination, morphogenesis and cell biogenesis, senescence, and programmed cell death. In this work, the main proteases that take part in these processes are reviewed. Proteolysis is also an important component together with protein oxidation in oxidative stress situations induced by senescence and heavy metals. The presence of exo- and endoproteolytic activity in plant peroxisomes is analyzed, and the role of peroxisomal proteases in different physiological events that take place under oxidative stress situations is discussed.
... ou de Pondal. A partir de agora débese engadir a esa nómina o traballo de Aguirre del 13ío, q... more ... ou de Pondal. A partir de agora débese engadir a esa nómina o traballo de Aguirre del 13ío, que sempre se citou como per-dido e que nesta súa primeira edición ofrecemos felizmente ó publico interesado. О manuscrito de ...
Free Radical Res, 1999
The effect of growing pea plants with 50 microM CdCl2 on the activated oxygen metabolism was stud... more The effect of growing pea plants with 50 microM CdCl2 on the activated oxygen metabolism was studied at subcellular level in peroxisomes isolated from pea leaves. Cadmium treatment produced proliferation of peroxisomes as well as an increase in the content of H2O2 in peroxisomes from pea leaves, but in peroxisomal membranes no significant effect on the NADH-dependent O2*- production was observed. The rate of lipid peroxidation of membranes was slightly decreased in peroxisomes from Cd-treated plants. This could be due to the Cd-induced increase in the activity of some antioxidative enzymes involved in H2O2 removal, mainly ascorbate peroxidase and glutathione reductase, as well as the NADP-dependent dehydrogenases present in these organelles. The activity of xanthine oxidase did not experiment changes by Cd treatment and this suggests that O2*- production in the peroxisomal matrix is not involved in Cd toxicity. This was supported by the absence of changes in plants treated with Cd in the Mn-SOD activity, responsible for O2*- removal in the peroxisomal matrix. Results obtained indicate that toxic Cd levels induce imbalances in the activated oxygen metabolism of pea leaf peroxisomes, but its main effect is an enhancement of the H2O2 concentration of these organelles. Peroxisomes respond to Cd toxicity by increasing the activity of antioxidative enzymes involved in the ascorbate-glutathione cycle and the NADP-dependent dehydrogenases located in these organelles.
Free Radical Research, Feb 1, 1997
Cytosolic copperzinc-superoxide dismutase (CuZn-SOD I; EC 1.15.1.1) was purified to homogeneity f... more Cytosolic copperzinc-superoxide dismutase (CuZn-SOD I; EC 1.15.1.1) was purified to homogeneity from watermelon (Citrullus vulgaris Schrad.) cotyledons. The stepwise purification procedure consisted of acetone precipitation, batch anion-exchange chromatography, anion-exchange Fast Protein Liquid Chromatography, gel-filtration column chromatography, and affinity chromatography on concanavalin A-Sepharose. CuZn-SOD I was purified 310-fold with a yield of 12.6 micrograms enzyme per gram cotyledons, and had a specific activity of 3,450 units per milligram protein. The relative molecular mass for cytosolic CuZn-SOD was 34000, and it was composed by two equal subunits of 16.3 kDa. CuZn-SOD I did not contain neutral carbohydrates in its molecule, and its ultraviolet and visible absorption spectra showed two absorption maxima at 254 nm and 580 nm. Metal analysis showed that the enzyme contained 1 gram-atom Cu and 1 gram-atom Zn per mole dimer. Cytosolic CuZn-SOD was recognized by the antibody against peroxisomal CuZn-SOD from watermelon cotyledons, and its enzymatic activity was inhibited by this antibody. By IEF (pH 4.2-4.9), using a new method for vertical slab gels set up in our laboratory, purified cytosolic CuZn-SOD was resolved into two equal isoforms with isoelectric point of 4.63 and 4.66.
Http Dx Doi Org 10 4161 Psb 3 5 5277, May 1, 2008
In animal cells, nitric oxide and NO-derived molecules have been shown to mediate post-translatio... more In animal cells, nitric oxide and NO-derived molecules have been shown to mediate post-translational modifications such as S-nitrosylation and protein tyrosine nitration which are associated with cell signalling and pathological processes, respectively. In plant cells, knowledge of the function of these post-translational modifications under physiological and stress conditions is still very rudimentary. In this addendum, we briefly examine how reactive nitrogen species (RNS) can exert important effects on proteins that could mediate signalling processes in plants.
Plant Physiology, Aug 1, 1989
The activity and isozymic composition of superoxide dismutase (SOD; EC 1.15.1.1) were determined ... more The activity and isozymic composition of superoxide dismutase (SOD; EC 1.15.1.1) were determined in nodules of Phaseolus vulgaris L., Pisum sativum L., and Vigna ungukulata (L.) Walp. formed by Rhizoblum phaseoll 3622, R. leguminosarum 3855, and Bradyrhizobium sp. BR7301, respectively. A Mn-SOD was present in Rhizobium and two in Bradyrhizobium and bacteroids. Nodule mitochondria from all three legume species had a single Mn-SOD with similar relative mobility, whereas the cytosol contained several CuZn-SODs: two in Phaseolus and Pisum, and four in Vigna.
Horticultura Revista De Industria Distribucion Y Socioeconomia Horticola Frutas Hortalizas Flores Plantas Arboles Ornamentales Y Viveros, 2007
Free Radical Res, 1997
In previous works using cell fractionation methods we demonstrated the presence of a Cu,Zn-contai... more In previous works using cell fractionation methods we demonstrated the presence of a Cu,Zn-containing superoxide dismutase in peroxisomes from watermelon cotyledons. In this work, this intracellular localization was evaluated by using western blot and EM immunocytochemical analysis with a polyclonal antibody against peroxisomal Cu,Zn-SOD II from watermelon cotyledons. In crude extracts from 6-day old cotyledons, analysis by western blot showed two cross-reactivity bands which belonged to the isozymes Cu,Zn-SOD I and Cu,Zn-SOD II. In peroxisomes purified by sucrose density-gradient centrifugation only one cross-reactivity band was found in the peroxisomal matrix which corresponded to the isozyme Cu,Zn-SOD II. When SOD activity was assayed in purified peroxisomes two isozymes were detected, Cu,Zn-SOD II in the matrix, and a Mn-SOD in the membrane fraction which was removed by sodium carbonate washing. EM immunocytochemistry of Cu,Zn-SOD on sections of 6-day old cotyledons, showed that gold label was mainly localized over plastids and also in peroxisomes and the cytosol, whereas mitochondria did not label for Cu,Zn-SOD.
... Tal sistema debería proporcionar las siguientes ventajas: - Una plataforma de tecnología inte... more ... Tal sistema debería proporcionar las siguientes ventajas: - Una plataforma de tecnología integrada que se añadiría a las inver-siones ya realizadas por una organización, para proporcionar infor-mación de alta calidad a cada PC o servidor del departamento, aña-diendo ...
Revista Espanola De Drogodependencias, 2006
Plant and Soil, May 1, 2010