Münir Aktas - Academia.edu (original) (raw)
Papers by Münir Aktas
Babesia ovis, commonly associated with ovine babesiosis, poses a significant threat to sheep heal... more Babesia ovis, commonly associated with ovine babesiosis, poses a significant threat to sheep health, often resulting in severe clinical manifestations and high mortality rates. However, the impact of B. ovis on goats has remained uncertain, prompting us to investigate its pathogenicity in caprine hosts. Experimental infections using B. ovis-infected blood inoculation and infected tick infestation, were conducted on spleen-intact (n=5) and splenectomized (n=5) goats. The experimental infection was performed using fresh blood obtained from a B. ovisinfected splenectomized sheep. One spleen-intact sheep served as a control for the experimental infection with B. ovis-infected Rhipicephalus bursa ticks. While all experimentally infected sheep (#501, #575) displayed severe clinical symptoms and high parasitemia, goats exhibited resistance, showing no significant clinical manifestations or sustained parasitemia. Notably, B. ovis was detected in two spleen-intact goats via nested PCR, prompting further investigation into their role as reservoirs for tick-borne transmission. These goats were then infested with Babesia spp.-free R. bursa larvae (0.1 gr) and adults (50 females and 50 males) for transstadial and transovarial transmission experiments respectively. Results indicated that chronically B. ovis-infected spleen-intact goats are not significant sources for maintaining the tick-borne transmission cycle of the parasite. These findings highlight the differential susceptibility of goats to B. ovis infection compared to sheep and their limited role as reservoirs for parasite transmission. Understanding the role of goats in B. ovis transmission and their resistance mechanisms can inform effective control measures and reduce economic losses in affected regions. Further research into caprine babesiosis and host immunological responses is essential to fully elucidate their possible role as reservoirs of the parasite, and underlying mechanisms of host susceptibility and parasite pathogenesis.
Veterinary Parasitology, Apr 1, 2015
In this study, asymptomatic dogs in nine provinces of Turkey were surveyed to investigate the pre... more In this study, asymptomatic dogs in nine provinces of Turkey were surveyed to investigate the prevalence and intensity of Hepatozoon canis infection. DNA obtained from blood samples collected from 694 domestic dogs (243 stray, 288 shelter, and 163 pets) of both genders and varying ages were evaluated by polymerase chain reaction (PCR). In addition, 285 thin blood smears prepared from these blood samples were also evaluated for microscopic examination. Direct microscopy revealed Hepatozoon gamonts in the peripheral blood of three of 285 (1.0%; 95% confidence interval (CI): 0.21-3.04) tested. Using PCR, 155 of the 694 (22.3%; 95% CI: 19.28-25.61) were found to be positive for the presence of H. canis DNA. The prevalence of infection was higher in adult dogs (26.2%; 95% CI: 22.1-30.7) than young animals (16.4%; 95% CI: 12.2-21.3). Although the prevalence determined by PCR was higher in male dogs (24.5%; 95% CI: 19.6-29.9) than in female dogs (20.8%; 95% CI: 16.9-25.1), gender differences were not significant. Pet dogs had a lower prevalence of infection (10.4%; 95% CI: 6.2-16.2) compared to stray (26.3%; 95% CI: 20.9-32.3) and shelter dogs (25.7%; 95% CI: 20.7-31.1), but no significant association between stray and shelter dogs was found for the presence of the parasite. Partial sequences of the 18S ribosomal RNA (rRNA) gene shared 99-100% similarity with the corresponding H. canis isolates. This epidemiological survey revealed a high prevalence of H. canis in dogs from several provinces in Turkey, and it suggests that the age and origin are associated with the parasite.
Pathogens
A novel Babesia sp. infecting goats was discovered based on the molecular findings obtained in th... more A novel Babesia sp. infecting goats was discovered based on the molecular findings obtained in the current study, which was conducted in the Mediterranean region of Türkiye. The goal of this study was to isolate this species of Babesia (Babesia sp.) infecting goats in vivo and to assess the genetic and morphological characterization of the parasite. To identify the animal naturally infected with Babesia sp. and isolate the parasite from this animal, field studies were conducted first, and genomic DNA were extracted from blood samples taken from goats (n = 50). The Theileria, Babesia, and Anaplasma species were identified using a nested PCR-based reverse line blotting (RLB) method. The study included one goat that was determined to be infected with Babesia sp. (single infection) in RLB for in vivo isolation. A blood smear was prepared to examine the parasite’s morphology, but it was found to be negative microscopically. Following that, a splenectomy operation (to suppress the immune ...
Pathogens
The ikeda and chitose genotypes of Theileria orientalis, which for many years were thought to be ... more The ikeda and chitose genotypes of Theileria orientalis, which for many years were thought to be benign, cause a disease that results in significant economic losses in the cattle industry. This study was carried out in order to determine the genotypes of T. orientalis in cattle in Kyrgyzstan, and 149 archived DNA samples known to be T. orientalis were analyzed by the PCR amplification of the major piroplasm surface protein (MPSP) gene region. Single-Strand Conformation Polymorphism (SSCP) analysis was performed to uncover the nucleotide changes in the archived DNA samples, and 15 samples showing different band profiles were subjected to sequence analysis. As a result of the sequence analysis, it was seen that the samples belonged to the buffeli and chitose A genotypes. In order to identify mixed genotypes, PCR was performed using primers specific for these genotypes, and buffeli (type 3), chitose (type 1) and buffeli+chitose were found to be positive in 26.2%, 2% and 71.8% of sample...
Turk. J. Vet. Anim …, 2001
Abstract: This study was carried out in cattle in Elazığ, Malatya and Tunceli provinces between M... more Abstract: This study was carried out in cattle in Elazığ, Malatya and Tunceli provinces between May 1997 and March 1998 to determine the seroprevalence of Babesia species. Serum samples were collected from a total of 741 cattle in Elazığ, Malatya, Tunceli and ...
Türkiye parazitoloji dergisi, 2011
Türkiye'de kırmızı tilkilerde endoparazit olarak Alaria alata, Mesocestoides lineatus, Taeni... more Türkiye'de kırmızı tilkilerde endoparazit olarak Alaria alata, Mesocestoides lineatus, Taenia multiceps, Taenia pisiformis, Taenia taeniaformis, Taenia spp., Echinococcus granulosus, Toxascaris leonina, Toxocara canis, Capillaria spp., Joyeuxiella echinorhynchoides, Taenia ...
Ankara Universitesi Veteriner Fakultesi Dergisi, Jun 14, 2019
Tick-borne pathogens cause serious health problems and loss of productivity in domesticated and w... more Tick-borne pathogens cause serious health problems and loss of productivity in domesticated and wild animals. A molecular study was performed to detect the frequency of infection with Anaplasma/Ehrlichia (A/E) in cattle from Karaman province of Turkey. Venous blood samples were taken from 150 apparently healthy cattle in 2016. After amplification the hypervariable V1 region of the 16S rRNA gene of A/E species, a reverse line blot (RLB) assay was performed using species-specific probes. Since some samples gave signal only to A/E catch-all probe, the samples analyzed in terms of major surface proteins (MSPs) of Anaplasma marginale. Genetic diversity and tandem repeat analysis were made for msp1α gene sequences of A. marginale. Anaplasma-like bodies were detected in four (2.66%) animals via microscopic examination. Anaplasma centrale was detected in eight (5.33%) animals via RLB. When the samples were examined in terms of A. marginale msp1a gene with semi-nested PCR, a total of nine (6.00%) animals [six of them (4.00%) were positive for A. centrale with RLB] were found to be infected with A. marginale. In addition, the sequences of MSP1a amplicons revealed one new tandem repeat (Tr70). According to these results, it was determined that A. marginale and A. centrale were found in cattle in Karaman province and this study provided the first evidence of genetic diversity of A. marginale with one new tandem repeat in cattle in the region.
Veterinary Parasitology, Nov 1, 2005
A pair of oligonucleotide primers were designed according to the nucleotide sequence of the P18 g... more A pair of oligonucleotide primers were designed according to the nucleotide sequence of the P18 gene of Babesia gibsoni (B. gibsoni), NRCPD strain, and were used to detect parasite DNA from blood samples of B. gibsoni-infected dogs by polymerase chain reaction (PCR). PCR was specific for B. gibsoni since no amplification was detected with DNA from B. canis or normal dog leucocytes. PCR was sensitive enough to detect parasite DNA from 2.5 µl of blood samples with a parasitemia of 0.000002%. PCR detected parasite DNA from 2 to 222 days post-infection in sequential blood samples derived from a dog experimentally infected with B. gibsoni. The detection of B. gibsoni DNA by PCR was much earlier than the detection of antibodies to B. gibsoni in blood samples by the indirect fluorescent antibody test (IFAT) or that of the parasite itself in Giemsa-stained thin blood smear film examined by microscopy. In addition, 28 field samples collected from dogs in Kansai area, Japan, were tested for B. gibsoni infection. Nine samples were positive in blood smears, 9 samples were positive by IFAT and 11 samples were positive for B. gibsoni DNA by PCR. The nucleotide sequences of PCR products from all 11 samples found positive by PCR were completely identical to that of the P18 gene of the B. gibsoni, NRCPD strain. These results suggest that PCR provides a useful diagnostic tool for the detection of B. gibsoni infection in dogs.
Ticks and Tick-borne Diseases, Mar 1, 2011
Bovine anaplasmosis is a tick-borne rickettsial disease widespread in tropical and subtropical ar... more Bovine anaplasmosis is a tick-borne rickettsial disease widespread in tropical and subtropical areas. We investigated the presence and distribution of Anaplasma spp. in cattle from 6 provinces in Turkey. For amplification of the segment spanning the V1 region of the 16S ribosomal RNA (rRNA) gene of Anaplasma species, a reverse line blot (RLB) hybridization assay was performed on 389 blood samples. RLB identified Anaplasma infections in 9.0% (35/389) of the samples. The most frequently found species was A. marginale (11/389, 2.8%), followed by A. centrale (4/389, 1.0%) and A. phagocytophilum (4/389, 1.0%). Eighteen of 35 PCR-positive samples gave positive signals to the catch-all probes, but did not show any response to the species-specific probes tested. Sequencing results of 5 representative amplicons randomly selected from these specimens indicated that 3 were 100% identical to the sequence of A. ovis, and the other 2 sequences were 99.5% identical to the sequence of Ehrlichia sp. Omatjenne strain. The results further confirmed that A. ovis and Ehrlichia sp. Omatjenne infection occurs in cattle populations in Turkey.
Bu derlemede kenelerin sistemdeki yeri, genel morfolojik ve biyolojik ozellikleri, beslenme, kene... more Bu derlemede kenelerin sistemdeki yeri, genel morfolojik ve biyolojik ozellikleri, beslenme, kene-konak-patojen iliskileri, vektorluk (Mekanik ve Biyolojik nakil), arthropodlarin patojenlerle enfeksiyonu, hastalik etkenlerinin vektorlerden omurgali konaklara nakli ve kenelerle tasinan onemli hastaliklar uzerinde durulmustur.
Eurasian Journal of Veterinary Sciences, Apr 4, 2004
This study was carried oul in eats in Elazig. A total of 36 eals were examined for the presence o... more This study was carried oul in eats in Elazig. A total of 36 eals were examined for the presence of anti-Toxoplasma gondii antibodies using Sabin-Felclman dye test (SF). Anti-Toxoplasma gondii antibodies were detected in 20 (55.5%) of the eats. Titers ol 1/16, 1164 were found in 9 (45%) and 11 (55%) ol the seropozitive ea ts res-pectively, Toxoplasma gondii oocyst was not found in the faeces examination.
Turkiye Klinikleri Journal of Infectious Diseases Special Topics, 2014
![Research paper thumbnail of Corrigendum to “Molecular detection of Theileria and Babesia infections in cattle” [Veterinary Parasitol. 158(4) (2008) 295–301]](https://attachments.academia-assets.com/115797655/thumbnails/1.jpg)
](Veterinary Parasitology, 2009
Türkiye parazitolojii dergisi / Türkiye Parazitoloji Derneği = Acta parasitologica Turcica / Turkish Society for Parasitology, 2007
Tams1 is a merozoite surface antigen of Theileria annulata. Genetical variations of Tams1 make st... more Tams1 is a merozoite surface antigen of Theileria annulata. Genetical variations of Tams1 make studies of vaccine and diagnostic tests like ELISA difficult. In this study, Tams1 genes of 89 T. annulata isolates obtained from natural infected cattle in Elazig and Bingöl regions were tested with PCR-RFLP. Six different restriction profiles (a, b, c, d, e, f) were detected. The number of restriction profiles of 89 samples was found to be as follows: 78(a), 2(b), 2(c), 5(d), 1(e), and 1(f).
Türkiye parazitolojii dergisi / Türkiye Parazitoloji Derneği = Acta parasitologica Turcica / Turkish Society for Parasitology, 2007
This study was carried out to investigate Theileria annulata and T. buffeli/orientalis in cattle ... more This study was carried out to investigate Theileria annulata and T. buffeli/orientalis in cattle in the region of Erzincan using reverse line blotting (RLB) and microscopical examination. A total of 123 blood samples and thin blood smears were collected from cattle in distinct locations. Thin blood smears were microscopically examined for Theileria piroplasms. The 18S SSU rRNA gene in the DNA of Theileria spp extracted from blood was amplified and used in RLB. For this purpose, PCR products were hybridized with specific probes for over-all Theileria spp., T. annulata and T. buffeli/orientalis as well as Babesia spp. While Theileria spp. were observed in 14 out of 123 cattle, (11.38 %) during microscopical examination, T. annulata was detected in 19 (15.45%) cattle and T. buffeli/orientalis, in 12 (9.76%) by RLB, respectively. Mixed infection was also detected in three samples.
Türkiye parazitolojii dergisi / Türkiye Parazitoloji Derneği = Acta parasitologica Turcica / Turkish Society for Parasitology, 2005
This study was carried out in order to investigate the presence of Theileria ovis in small rumina... more This study was carried out in order to investigate the presence of Theileria ovis in small ruminants in the Elazig region between April-October 2004. A total of 164 whole blood and thin blood smears (from 103 sheep and 61 goats) were collected from 15 flocks in different locations. T. ovis piroplasm DNA extracted from sheep and goats' blood was amplified by the polymerase chain reaction (PCR) using specific primers. Thin blood smears were examined for Theileria piroplasms by microscopic examination. In the examination of DNA extracted from 103 sheep and 61 goats, amplification with the molecular length 520 base pair was obtained in 67.96% (70/103) and 1.63% (1/61), respectively. In the microscopic examination of thin blood smears, Theileria spp. were observed in 40 out of 103 sheep (38.83%), but Theileria spp. were not seen in goats. The difference between microscopic examination and the PCR results was statistically significant (p < 0.01).
Türkiye parazitolojii dergisi / Türkiye Parazitoloji Derneği = Acta parasitologica Turcica / Turkish Society for Parasitology, 2008
The species causing theileriosis in cattle in Turkey are Theileria annulata and T. buffeli. While... more The species causing theileriosis in cattle in Turkey are Theileria annulata and T. buffeli. While T. buffeli is low in pathogenicity or non-pathogenic , T. annulata is very pathogenic and causes tropical theileriosis with high morbidity and mortality in cattle. In this study, a multiplex PCR was used for a simultaneous diagnosis of these species. Genes for the merozoite surface antigen (Tams 1) and the major piroplasm surface protein (MPSP) were amplified with PCR for T. annulata and T. buffeli, respectively. It was found that both single and mixed infection with T. annulata and T. buffeli could be diagnosed with multiplex PCR.
Babesia ovis, commonly associated with ovine babesiosis, poses a significant threat to sheep heal... more Babesia ovis, commonly associated with ovine babesiosis, poses a significant threat to sheep health, often resulting in severe clinical manifestations and high mortality rates. However, the impact of B. ovis on goats has remained uncertain, prompting us to investigate its pathogenicity in caprine hosts. Experimental infections using B. ovis-infected blood inoculation and infected tick infestation, were conducted on spleen-intact (n=5) and splenectomized (n=5) goats. The experimental infection was performed using fresh blood obtained from a B. ovisinfected splenectomized sheep. One spleen-intact sheep served as a control for the experimental infection with B. ovis-infected Rhipicephalus bursa ticks. While all experimentally infected sheep (#501, #575) displayed severe clinical symptoms and high parasitemia, goats exhibited resistance, showing no significant clinical manifestations or sustained parasitemia. Notably, B. ovis was detected in two spleen-intact goats via nested PCR, prompting further investigation into their role as reservoirs for tick-borne transmission. These goats were then infested with Babesia spp.-free R. bursa larvae (0.1 gr) and adults (50 females and 50 males) for transstadial and transovarial transmission experiments respectively. Results indicated that chronically B. ovis-infected spleen-intact goats are not significant sources for maintaining the tick-borne transmission cycle of the parasite. These findings highlight the differential susceptibility of goats to B. ovis infection compared to sheep and their limited role as reservoirs for parasite transmission. Understanding the role of goats in B. ovis transmission and their resistance mechanisms can inform effective control measures and reduce economic losses in affected regions. Further research into caprine babesiosis and host immunological responses is essential to fully elucidate their possible role as reservoirs of the parasite, and underlying mechanisms of host susceptibility and parasite pathogenesis.
Veterinary Parasitology, Apr 1, 2015
In this study, asymptomatic dogs in nine provinces of Turkey were surveyed to investigate the pre... more In this study, asymptomatic dogs in nine provinces of Turkey were surveyed to investigate the prevalence and intensity of Hepatozoon canis infection. DNA obtained from blood samples collected from 694 domestic dogs (243 stray, 288 shelter, and 163 pets) of both genders and varying ages were evaluated by polymerase chain reaction (PCR). In addition, 285 thin blood smears prepared from these blood samples were also evaluated for microscopic examination. Direct microscopy revealed Hepatozoon gamonts in the peripheral blood of three of 285 (1.0%; 95% confidence interval (CI): 0.21-3.04) tested. Using PCR, 155 of the 694 (22.3%; 95% CI: 19.28-25.61) were found to be positive for the presence of H. canis DNA. The prevalence of infection was higher in adult dogs (26.2%; 95% CI: 22.1-30.7) than young animals (16.4%; 95% CI: 12.2-21.3). Although the prevalence determined by PCR was higher in male dogs (24.5%; 95% CI: 19.6-29.9) than in female dogs (20.8%; 95% CI: 16.9-25.1), gender differences were not significant. Pet dogs had a lower prevalence of infection (10.4%; 95% CI: 6.2-16.2) compared to stray (26.3%; 95% CI: 20.9-32.3) and shelter dogs (25.7%; 95% CI: 20.7-31.1), but no significant association between stray and shelter dogs was found for the presence of the parasite. Partial sequences of the 18S ribosomal RNA (rRNA) gene shared 99-100% similarity with the corresponding H. canis isolates. This epidemiological survey revealed a high prevalence of H. canis in dogs from several provinces in Turkey, and it suggests that the age and origin are associated with the parasite.
Pathogens
A novel Babesia sp. infecting goats was discovered based on the molecular findings obtained in th... more A novel Babesia sp. infecting goats was discovered based on the molecular findings obtained in the current study, which was conducted in the Mediterranean region of Türkiye. The goal of this study was to isolate this species of Babesia (Babesia sp.) infecting goats in vivo and to assess the genetic and morphological characterization of the parasite. To identify the animal naturally infected with Babesia sp. and isolate the parasite from this animal, field studies were conducted first, and genomic DNA were extracted from blood samples taken from goats (n = 50). The Theileria, Babesia, and Anaplasma species were identified using a nested PCR-based reverse line blotting (RLB) method. The study included one goat that was determined to be infected with Babesia sp. (single infection) in RLB for in vivo isolation. A blood smear was prepared to examine the parasite’s morphology, but it was found to be negative microscopically. Following that, a splenectomy operation (to suppress the immune ...
Pathogens
The ikeda and chitose genotypes of Theileria orientalis, which for many years were thought to be ... more The ikeda and chitose genotypes of Theileria orientalis, which for many years were thought to be benign, cause a disease that results in significant economic losses in the cattle industry. This study was carried out in order to determine the genotypes of T. orientalis in cattle in Kyrgyzstan, and 149 archived DNA samples known to be T. orientalis were analyzed by the PCR amplification of the major piroplasm surface protein (MPSP) gene region. Single-Strand Conformation Polymorphism (SSCP) analysis was performed to uncover the nucleotide changes in the archived DNA samples, and 15 samples showing different band profiles were subjected to sequence analysis. As a result of the sequence analysis, it was seen that the samples belonged to the buffeli and chitose A genotypes. In order to identify mixed genotypes, PCR was performed using primers specific for these genotypes, and buffeli (type 3), chitose (type 1) and buffeli+chitose were found to be positive in 26.2%, 2% and 71.8% of sample...
Turk. J. Vet. Anim …, 2001
Abstract: This study was carried out in cattle in Elazığ, Malatya and Tunceli provinces between M... more Abstract: This study was carried out in cattle in Elazığ, Malatya and Tunceli provinces between May 1997 and March 1998 to determine the seroprevalence of Babesia species. Serum samples were collected from a total of 741 cattle in Elazığ, Malatya, Tunceli and ...
Türkiye parazitoloji dergisi, 2011
Türkiye'de kırmızı tilkilerde endoparazit olarak Alaria alata, Mesocestoides lineatus, Taeni... more Türkiye'de kırmızı tilkilerde endoparazit olarak Alaria alata, Mesocestoides lineatus, Taenia multiceps, Taenia pisiformis, Taenia taeniaformis, Taenia spp., Echinococcus granulosus, Toxascaris leonina, Toxocara canis, Capillaria spp., Joyeuxiella echinorhynchoides, Taenia ...
Ankara Universitesi Veteriner Fakultesi Dergisi, Jun 14, 2019
Tick-borne pathogens cause serious health problems and loss of productivity in domesticated and w... more Tick-borne pathogens cause serious health problems and loss of productivity in domesticated and wild animals. A molecular study was performed to detect the frequency of infection with Anaplasma/Ehrlichia (A/E) in cattle from Karaman province of Turkey. Venous blood samples were taken from 150 apparently healthy cattle in 2016. After amplification the hypervariable V1 region of the 16S rRNA gene of A/E species, a reverse line blot (RLB) assay was performed using species-specific probes. Since some samples gave signal only to A/E catch-all probe, the samples analyzed in terms of major surface proteins (MSPs) of Anaplasma marginale. Genetic diversity and tandem repeat analysis were made for msp1α gene sequences of A. marginale. Anaplasma-like bodies were detected in four (2.66%) animals via microscopic examination. Anaplasma centrale was detected in eight (5.33%) animals via RLB. When the samples were examined in terms of A. marginale msp1a gene with semi-nested PCR, a total of nine (6.00%) animals [six of them (4.00%) were positive for A. centrale with RLB] were found to be infected with A. marginale. In addition, the sequences of MSP1a amplicons revealed one new tandem repeat (Tr70). According to these results, it was determined that A. marginale and A. centrale were found in cattle in Karaman province and this study provided the first evidence of genetic diversity of A. marginale with one new tandem repeat in cattle in the region.
Veterinary Parasitology, Nov 1, 2005
A pair of oligonucleotide primers were designed according to the nucleotide sequence of the P18 g... more A pair of oligonucleotide primers were designed according to the nucleotide sequence of the P18 gene of Babesia gibsoni (B. gibsoni), NRCPD strain, and were used to detect parasite DNA from blood samples of B. gibsoni-infected dogs by polymerase chain reaction (PCR). PCR was specific for B. gibsoni since no amplification was detected with DNA from B. canis or normal dog leucocytes. PCR was sensitive enough to detect parasite DNA from 2.5 µl of blood samples with a parasitemia of 0.000002%. PCR detected parasite DNA from 2 to 222 days post-infection in sequential blood samples derived from a dog experimentally infected with B. gibsoni. The detection of B. gibsoni DNA by PCR was much earlier than the detection of antibodies to B. gibsoni in blood samples by the indirect fluorescent antibody test (IFAT) or that of the parasite itself in Giemsa-stained thin blood smear film examined by microscopy. In addition, 28 field samples collected from dogs in Kansai area, Japan, were tested for B. gibsoni infection. Nine samples were positive in blood smears, 9 samples were positive by IFAT and 11 samples were positive for B. gibsoni DNA by PCR. The nucleotide sequences of PCR products from all 11 samples found positive by PCR were completely identical to that of the P18 gene of the B. gibsoni, NRCPD strain. These results suggest that PCR provides a useful diagnostic tool for the detection of B. gibsoni infection in dogs.
Ticks and Tick-borne Diseases, Mar 1, 2011
Bovine anaplasmosis is a tick-borne rickettsial disease widespread in tropical and subtropical ar... more Bovine anaplasmosis is a tick-borne rickettsial disease widespread in tropical and subtropical areas. We investigated the presence and distribution of Anaplasma spp. in cattle from 6 provinces in Turkey. For amplification of the segment spanning the V1 region of the 16S ribosomal RNA (rRNA) gene of Anaplasma species, a reverse line blot (RLB) hybridization assay was performed on 389 blood samples. RLB identified Anaplasma infections in 9.0% (35/389) of the samples. The most frequently found species was A. marginale (11/389, 2.8%), followed by A. centrale (4/389, 1.0%) and A. phagocytophilum (4/389, 1.0%). Eighteen of 35 PCR-positive samples gave positive signals to the catch-all probes, but did not show any response to the species-specific probes tested. Sequencing results of 5 representative amplicons randomly selected from these specimens indicated that 3 were 100% identical to the sequence of A. ovis, and the other 2 sequences were 99.5% identical to the sequence of Ehrlichia sp. Omatjenne strain. The results further confirmed that A. ovis and Ehrlichia sp. Omatjenne infection occurs in cattle populations in Turkey.
Bu derlemede kenelerin sistemdeki yeri, genel morfolojik ve biyolojik ozellikleri, beslenme, kene... more Bu derlemede kenelerin sistemdeki yeri, genel morfolojik ve biyolojik ozellikleri, beslenme, kene-konak-patojen iliskileri, vektorluk (Mekanik ve Biyolojik nakil), arthropodlarin patojenlerle enfeksiyonu, hastalik etkenlerinin vektorlerden omurgali konaklara nakli ve kenelerle tasinan onemli hastaliklar uzerinde durulmustur.
Eurasian Journal of Veterinary Sciences, Apr 4, 2004
This study was carried oul in eats in Elazig. A total of 36 eals were examined for the presence o... more This study was carried oul in eats in Elazig. A total of 36 eals were examined for the presence of anti-Toxoplasma gondii antibodies using Sabin-Felclman dye test (SF). Anti-Toxoplasma gondii antibodies were detected in 20 (55.5%) of the eats. Titers ol 1/16, 1164 were found in 9 (45%) and 11 (55%) ol the seropozitive ea ts res-pectively, Toxoplasma gondii oocyst was not found in the faeces examination.
Turkiye Klinikleri Journal of Infectious Diseases Special Topics, 2014
Veterinary Parasitology, 2009
Türkiye parazitolojii dergisi / Türkiye Parazitoloji Derneği = Acta parasitologica Turcica / Turkish Society for Parasitology, 2007
Tams1 is a merozoite surface antigen of Theileria annulata. Genetical variations of Tams1 make st... more Tams1 is a merozoite surface antigen of Theileria annulata. Genetical variations of Tams1 make studies of vaccine and diagnostic tests like ELISA difficult. In this study, Tams1 genes of 89 T. annulata isolates obtained from natural infected cattle in Elazig and Bingöl regions were tested with PCR-RFLP. Six different restriction profiles (a, b, c, d, e, f) were detected. The number of restriction profiles of 89 samples was found to be as follows: 78(a), 2(b), 2(c), 5(d), 1(e), and 1(f).
Türkiye parazitolojii dergisi / Türkiye Parazitoloji Derneği = Acta parasitologica Turcica / Turkish Society for Parasitology, 2007
This study was carried out to investigate Theileria annulata and T. buffeli/orientalis in cattle ... more This study was carried out to investigate Theileria annulata and T. buffeli/orientalis in cattle in the region of Erzincan using reverse line blotting (RLB) and microscopical examination. A total of 123 blood samples and thin blood smears were collected from cattle in distinct locations. Thin blood smears were microscopically examined for Theileria piroplasms. The 18S SSU rRNA gene in the DNA of Theileria spp extracted from blood was amplified and used in RLB. For this purpose, PCR products were hybridized with specific probes for over-all Theileria spp., T. annulata and T. buffeli/orientalis as well as Babesia spp. While Theileria spp. were observed in 14 out of 123 cattle, (11.38 %) during microscopical examination, T. annulata was detected in 19 (15.45%) cattle and T. buffeli/orientalis, in 12 (9.76%) by RLB, respectively. Mixed infection was also detected in three samples.
Türkiye parazitolojii dergisi / Türkiye Parazitoloji Derneği = Acta parasitologica Turcica / Turkish Society for Parasitology, 2005
This study was carried out in order to investigate the presence of Theileria ovis in small rumina... more This study was carried out in order to investigate the presence of Theileria ovis in small ruminants in the Elazig region between April-October 2004. A total of 164 whole blood and thin blood smears (from 103 sheep and 61 goats) were collected from 15 flocks in different locations. T. ovis piroplasm DNA extracted from sheep and goats' blood was amplified by the polymerase chain reaction (PCR) using specific primers. Thin blood smears were examined for Theileria piroplasms by microscopic examination. In the examination of DNA extracted from 103 sheep and 61 goats, amplification with the molecular length 520 base pair was obtained in 67.96% (70/103) and 1.63% (1/61), respectively. In the microscopic examination of thin blood smears, Theileria spp. were observed in 40 out of 103 sheep (38.83%), but Theileria spp. were not seen in goats. The difference between microscopic examination and the PCR results was statistically significant (p < 0.01).
Türkiye parazitolojii dergisi / Türkiye Parazitoloji Derneği = Acta parasitologica Turcica / Turkish Society for Parasitology, 2008
The species causing theileriosis in cattle in Turkey are Theileria annulata and T. buffeli. While... more The species causing theileriosis in cattle in Turkey are Theileria annulata and T. buffeli. While T. buffeli is low in pathogenicity or non-pathogenic , T. annulata is very pathogenic and causes tropical theileriosis with high morbidity and mortality in cattle. In this study, a multiplex PCR was used for a simultaneous diagnosis of these species. Genes for the merozoite surface antigen (Tams 1) and the major piroplasm surface protein (MPSP) were amplified with PCR for T. annulata and T. buffeli, respectively. It was found that both single and mixed infection with T. annulata and T. buffeli could be diagnosed with multiplex PCR.