SHREYA MANDAL - Academia.edu (original) (raw)

Papers by SHREYA MANDAL

Methods: A survey research design with a quantitative research approach was used to conduct the c... more Methods: A survey research design with a quantitative research approach was used to conduct the current study. The study was conducted in orphanage centers and schools of Bhubaneswar, Odisha. For this study, 100 numbers (50 orphan & 50 non-orphan children) of the sample were selected by randomization. After getting the approval from the ethical committee and administrative permissions were taken from respective authorities, with that written consent was taken from the participants. The purpose and objectives were explained and the data were collected. Tool used for collecting data from samples were sociodemographic Performa and self-structured 3 point psychosocial development assessment scale.

Uremia, acute kidney injury (AKI), chronic kidney disease (CKD) and end-stage renal disease (ESRD... more Uremia, acute kidney injury (AKI), chronic kidney disease (CKD) and end-stage renal disease (ESRD) have become worldwide public health problems. There are many causes for acute renal failure which mainly includes acute tubular necrosis which occurs either due to ischemia or toxins. CKD or ESRD is a gradual decrease of kidney function in both kidneys over a period of time. These conditions increase patient morbidity and mortality risks and treatment option is only dialysis and kidney transplantation which is very much expensive and not free from side effects. So that the maximum people of our country cannot afford this therapy to treat kidney disorders. However, In absence of reliable and effective modern nephroprotective drugs and traditional medicines, different scientists in different part of the World is trying to employed for the treatment of the diseases by using complementary or alternative medicine of the disease treatment and /or prevention which are free from side effects a...

Indian journal of experimental biology, 2013

The total antioxidative activity of L. ingluviei ADK10 isolated from chicken intestine intact cel... more The total antioxidative activity of L. ingluviei ADK10 isolated from chicken intestine intact cells and cell free culture supernatant (CFCS) was 54- 67.95%. The ability to scavenge a,a-Diphenyl-b-Picrylhydrazyl free radical ranged from 71 and 64% in intact cells and CFCS respectively. Total reducing activity of bacteria was equivalent to 290 microM/L of cysteine. Reducing glutathione activity was equivalent to 93.95 microg/mL. Oral administration of the strain at a dose of 10(9) cfu/kg body weight to acetaminophen induced oxidative stress in rats increased catalase, glutathione and superoxide dismutase activity in the blood, liver and kidney and lowered malondialdehyde level. The results indicate that L. ingluviei ADK10 has potential free radical scavenging activity for the treatment of oxidative stress related disease.

International journal of pharma and bio sciences, 2016

Hyperlipidimia is a medical condition where there is an elevation of lipids, or fats, in the bloo... more Hyperlipidimia is a medical condition where there is an elevation of lipids, or fats, in the blood. The purpose of the study was to evaluate the hypolipidemic effect of sesame seed on experimentally induced hyperlipidemic albino rats. The study was carried out with thirty Wistar stain albino male rats and the groups were Group I was denoted as control, they were provided normal food, water with 1g multi-vitamin for 60 days. Group II was denoted as hyperlipidemic, from 1 st to 14 th day they were provided normal food, water and on the 15 th to 60 th day (45 days) they were provided normal food with 3.7 ml coconut oil with 1mg/kg cholesterol per rat, 1g multi-vitamin, and 5% sucrose for induction of hyperlipidemia. Group III, Group IV, Group V were denoted as treatment I, I, III respectively and they were treated as group II rats and fed orally sesame seed extract at the dose of 25, 50 and 75 mg/kg body weight/day/rat orally for 45 days respectively. The result of the present study sh...

Now days, herbal medicines are widely used to treat, manage and cure the kidney diseases as well ... more Now days, herbal medicines are widely used to treat, manage and cure the kidney diseases as well as reduce uremia, acute nephritis & nephritic syndrome. The present study was to evaluate the antiuremic and antioxidative effect of Asparagus racemosus (AR) on acetaminophen induced uremic male rats. The study was designed with 36 male albino rats which were randomly divided into 6 groups. Group I animals were provided normal food and water ad libitum, Group II, III, IV, V and VI received acetaminophen intraperitoneally at the dose of 500 mg/kg body weight/day for 10 days. Methanol, aqueous, hydromethanol, and hexane extract of AR at the dose of 500mg/kg body weight/day were fed orally on the 11 th day and continue for next 15 days to group III, IV, V and VI respectively. After 25 days, group-II animals showed significantly increased (p<0.05) plasma urea, creatinine, sodium. Elevation of lipid peroxidation was noted by measuring Malondialdehyde level in both plasma and kidney tissues...

The gastrointestinal tract of the human and other mammals is populated by a vast and diverse grou... more The gastrointestinal tract of the human and other mammals is populated by a vast and diverse group of microbes in a composite manner (Frank and Pace, 2008; Ito, 2005). Bacteria are the major population of the alimentary tract and this native microflora is commonly designated the gut microflora (Ito, 2005). Microbial flora present in the microenvironment of the gastrointestinal tract performs several important and essential activities of the host (Straw, 1989) such as the breakdown of undigested food, metabolism of drugs, enhanced absorption of foodstuffs, synthesis of vitamins, creation of resistance against pathogenic bacteria by colonization resistance, stimulation of host ISSN: 2319-7706 Volume 3 Number 8 (2014) pp. 282-291 http://www.ijcmas.com

Heart disease and Diabetes possess the first and second leading cause of death in the world and p... more Heart disease and Diabetes possess the first and second leading cause of death in the world and people are suffering from chronic kidney disease are likely more tend to suffer from hypercholesterolemia. Alpha-lipoic acid is unsaturated fatty acids having strong anti-oxidative properties. The aim of this study was to characterize alpha-lipoic acid as a cholesterol reducer for acetaminophen induced uremic rats. The study was performed on thirty male albino rats. Plasma total cholesterol, triglyceride levels, liver enzymes assay and hematologic parameters were performed to investigate its cholesterol reducing properties. In this present study it was observed that receiving acetaminophen intraperitoneally for 10 days significantly decreased Hb level and RBC count compared with control. The present study demonstrated that cholesterol and triglycerides levels were significantly higher in acetaminophen treated group compare to control. Alpha-lipoic acid ameliorates cholesterol and triglyce...

ARTICLE INFO ABSTRACT Article history Received 12 August 2016 Accepted 3 September 2016 Uremia, a... more ARTICLE INFO ABSTRACT Article history Received 12 August 2016 Accepted 3 September 2016 Uremia, acute kidney injury (AKI), chronic kidney disease (CKD) and end-stage renal disease (ESRD) have become worldwide public health problems. There are many causes for acute renal failure which mainly includes acute tubular necrosis which occurs either due to ischemia or toxins. CKD or ESRD is a gradual decrease of kidney function in both kidneys over a period of time. These conditions increase patient morbidity and mortality risks and treatment option is only dialysis and kidney transplantation which is very much expensive and not free from side effects. So that the maximum people of our country cannot afford this therapy to treat kidney disorders. However, In absence of reliable and effective modern nephroprotective drugs and traditional medicines, different scientists in different part of the World is trying to employed for the treatment of the diseases by using complementary or alternative...

Saudi Journal of Kidney Diseases and Transplantation, 2018

Oxygen is very important to the existence of life. Oxygen deficiency, defined as hypoxia, elicits... more Oxygen is very important to the existence of life. Oxygen deficiency, defined as hypoxia, elicits adaptive responses in cells and tissues. Lower oxygen concentration can cause the alteration of renal function, affects the maintenance of a balance of the body fluids, electrolytes, pH, and blood pressure homeostasis. Impaired fluid regulation could, in addition, contribute to the precipitation of pulmonary edema and exacerbate hypoxemia which may accelerate the progression of chronic kidney disease. In this context, the present study attempted to evaluate the association of renal injury and oxidative stress at different atmospheric pressures (1829, 3657, and 5486 m). Limited fecal analysis of experimental animals was also done to evaluate the impact of hypobaric hypoxia on the composition of dominant gastrointestinal microbiota. The study was performed on 24 male Wister strain rats and divided into four groups (C, HA-I, HA-II, and HA-III), and exposure was carried out for seven days period. In hypoxic exposure rats, plasma urea, creatinine, electrolytes and malonaldehyde level elevated and catalase and superoxide dismutase level diminished significantly compared to the controls. Increase in blood uremia profile, toxicity markers, and lipid peroxidation marker enzymes indicated that hypoxia causes renal failure. Histological structures of the kidney of group HA-II and HA-III animals showed severe disorganization of glomerulus and dilation of renal tubules. These results indicate nephrotoxicity or acute renal failure can occur at hypobaric hypoxia and it also affected the gut microbial population. This alteration was observed significantly above 3000 m.

International Journal of Pharmacy and Pharmaceutical Sciences, Dec 1, 2017

The aim of this study was to evaluate the antioxidant property of the isolated phytocompounds fro... more The aim of this study was to evaluate the antioxidant property of the isolated phytocompounds from TA (Terminalia arjuna) bark and in vivo study for nephro-protective and oxidative stress reducing activity in experimentally induced albino male rats. Methods: Fractions from methanol crude TA extract were collected by column chromatography and F27, F28, F29 fractions were selected on the basis of antioxidant property by 2,2-diphenyl-1-picrylhydrazyl (DPPH) scavenging assay. The in vivo study performed by 30 albino male rats which were randomly divided into five groups: Group I (control)were taken normal food and water, Groups II (uremic) were injected acetaminophen intraperitoneally at the dose of 500 mg/kg/d for 10 d, Group III, IV and V(extract treatment) acetaminophen intraperitoneally at the dose of 500 mg/kg/d for 10 d with co-administered orally of methanol fraction F27, F28, F29 at the dose of 100 mg/kg/d for 15 d respectively. Results: After scarification of rats, the uremic marker plasma urea (80%), creatinine (85%) were elevated and antioxidant enzyme marker such as plasma SOD and catalase level were significantly increased (p<0.05)in Group IV compared to Group II. The total phenolic content of the F28 methanolic fraction was (815.48±8.11) mg gallic acid equivalent/g of extract. For isolation of available compound by 1 Conclusion: Among the three methanolic fraction of TA bark, F28 was shown best antioxidative, nephron-protective and oxidative stress reducing property. H NMR study in F28 methanol fraction of TA bark was arjunoside IV which contained olefinic proton (a pair of carbon atom linked with double bond).

Acta Biologica Szegediensis, 2018

Acetaminophen-induced renal necrosis and insufficiency occurs in patients with acetaminophen over... more Acetaminophen-induced renal necrosis and insufficiency occurs in patients with acetaminophen overdose. Renal failure is rapidly assuming epidemic proportions globally. In absence of reliable and effective nephroprotective drugs, strategies towards exploring alternative therapies for treatment of kidney diseases are essential. Asparagus racemosus is a medicinal plant used for treatment of various ailments. This research was undertaken to investigate the protective effect of ethanol fraction of A. racemosus roots extract in acetaminophen-induced uraemia and renal failure in rats. Rats were co-administered with acetaminophen injection and oral administration of A. racemosus roots extract in an attempt of protection against renal failure. Uremic biomarkers significantly decreased, and elevated levels of antioxidant enzymes were found, in the animals treated with ethanol fraction of A. racemosus when compared with acetaminophen treated uremic animals. Also, histology of kidneys showed co...

Clinical Nutrition Experimental, 2018

International Journal of Research in Ayurveda & Pharmacy, 2017

The present study is designed to investigate the comparison level of antimicrobial, antioxidant p... more The present study is designed to investigate the comparison level of antimicrobial, antioxidant properties and as well as the total phenolic content estimation of aqueous extract between dusts and leaf black tea. Antimicrobial assay as well as antioxidant activity of phyto-compounds in both types of black tea was detected by using agar well diffusion and chromatographic technique respectively. Total antioxidant activity and phenolic content were measured by using coloriemetric method. In comparison level the antimicrobial activity and screening of phytochemical compounds like saponins, phenolic compounds & tannins, flavonoids, triterpenoids were significantly (P<0.05) different in both aqueous extract of leaf black tea and dust black tea. Total phenolic contents in the leaf black tea were 130.51mg Gallic acid equivalent/g of dried aqueous extract. In conclusion the leaf black tea has potential to be used as a natural antioxidant which is attributed to the rich presence of secondary metabolites and exhibit medicinal as well as physiological activities.

International Journal of Pharmacy and Pharmaceutical Sciences, Sep 1, 2014

Journal of Biological Sciences, 2015

Acetaminophen is a commonly used analgesic and antipyretic drug, high doses of which cause hepati... more Acetaminophen is a commonly used analgesic and antipyretic drug, high doses of which cause hepatic and renal injury. In development and progression of kidney disease research, it is necessary to have a suitable common drug to induce uremia and renal failure of rats. It is also required to select the threshold doses for the said drug; a therapeutic dose and toxic dose for kidney failure using standard guidelines. An acute toxicity of acetaminophen was conducted by the limit test at a dose of 2000 mg kgG 1 b.wt., on either sex rats (n = 5) and a main test was conducted by a dose progression factor of 3.2 times as per Organization of Economic CoOperation and Development guidelines 425. Eighteen male albino rats (n = 18) were divided into three groups, group I served as control, groups II and III rats were administered 175 mg and 550 mg kgG 1 b.wt., acetaminophen intraperitoneally for 14 days, respectively. Different parameters were considered to analyze renal failure. Urea, creatinine, GOT, GPT and MDA levels were increased significantly (p<0.05) in group III, compared to groups I and II. Antioxidant enzymes like SOD, catalase and GSH level were decreased significantly (p<0.05) in group III rats, compared to group I and II rats. Increase in blood uremia profile indicated that the higher dose of acetaminophen causes uremia. Increase in the toxicity markers and lipid peroxidation marker enzymes indicate the nephrotoxicity. Histological structures of kidney of group III animals showed a severe disorganization of glomerulus and dilation of renal tubules. These results indicate that intraperitoneal injection of acetaminophen at high dose causes nephrotoxicity and renal cellular damage to experimental rats.

Indian journal of experimental biology, 2013

The total antioxidative activity of L. ingluviei ADK10 isolated from chicken intestine intact cel... more The total antioxidative activity of L. ingluviei ADK10 isolated from chicken intestine intact cells and cell free culture supernatant (CFCS) was 54- 67.95%. The ability to scavenge a,a-Diphenyl-b-Picrylhydrazyl free radical ranged from 71 and 64% in intact cells and CFCS respectively. Total reducing activity of bacteria was equivalent to 290 microM/L of cysteine. Reducing glutathione activity was equivalent to 93.95 microg/mL. Oral administration of the strain at a dose of 10(9) cfu/kg body weight to acetaminophen induced oxidative stress in rats increased catalase, glutathione and superoxide dismutase activity in the blood, liver and kidney and lowered malondialdehyde level. The results indicate that L. ingluviei ADK10 has potential free radical scavenging activity for the treatment of oxidative stress related disease.

Asian Pacific journal of tropical biomedicine, 2013

To investigate phytochemical screening, antimicrobial activity and qualitative thin layer chromat... more To investigate phytochemical screening, antimicrobial activity and qualitative thin layer chromatographic separation of flavonoid components, antioxidant activity and total flavonoid compound of Terminalia arjuna. For phytochemical screening, some common and available standard tests were done. Antimicrobial bioassay was done through agar well diffusion method. Detection of antioxidant activity and flavonoid compounds were done through thin layer chromatography. Total antioxidant activity was measured by 2, 2-diphenyl-1-picrylhydrazyl (DPPH) in colorimetric method. Aluminum chloride colorimetric method was used for total flavonoid determination. Phytochemical screening showed the active compounds presence in high concentration, such as phytosterol, lactones, flavonoids, phenolic compounds and tannins and glycosides. The antimicrobial activity of extract showed that greater inhibition zone against Gram negative bacteria than Gram positive bacteria. This methanolic extract showed a pro...

Biomedicine & Preventive Nutrition, 2014

ABSTRACT

Methods: A survey research design with a quantitative research approach was used to conduct the c... more Methods: A survey research design with a quantitative research approach was used to conduct the current study. The study was conducted in orphanage centers and schools of Bhubaneswar, Odisha. For this study, 100 numbers (50 orphan & 50 non-orphan children) of the sample were selected by randomization. After getting the approval from the ethical committee and administrative permissions were taken from respective authorities, with that written consent was taken from the participants. The purpose and objectives were explained and the data were collected. Tool used for collecting data from samples were sociodemographic Performa and self-structured 3 point psychosocial development assessment scale.

Uremia, acute kidney injury (AKI), chronic kidney disease (CKD) and end-stage renal disease (ESRD... more Uremia, acute kidney injury (AKI), chronic kidney disease (CKD) and end-stage renal disease (ESRD) have become worldwide public health problems. There are many causes for acute renal failure which mainly includes acute tubular necrosis which occurs either due to ischemia or toxins. CKD or ESRD is a gradual decrease of kidney function in both kidneys over a period of time. These conditions increase patient morbidity and mortality risks and treatment option is only dialysis and kidney transplantation which is very much expensive and not free from side effects. So that the maximum people of our country cannot afford this therapy to treat kidney disorders. However, In absence of reliable and effective modern nephroprotective drugs and traditional medicines, different scientists in different part of the World is trying to employed for the treatment of the diseases by using complementary or alternative medicine of the disease treatment and /or prevention which are free from side effects a...

Indian journal of experimental biology, 2013

The total antioxidative activity of L. ingluviei ADK10 isolated from chicken intestine intact cel... more The total antioxidative activity of L. ingluviei ADK10 isolated from chicken intestine intact cells and cell free culture supernatant (CFCS) was 54- 67.95%. The ability to scavenge a,a-Diphenyl-b-Picrylhydrazyl free radical ranged from 71 and 64% in intact cells and CFCS respectively. Total reducing activity of bacteria was equivalent to 290 microM/L of cysteine. Reducing glutathione activity was equivalent to 93.95 microg/mL. Oral administration of the strain at a dose of 10(9) cfu/kg body weight to acetaminophen induced oxidative stress in rats increased catalase, glutathione and superoxide dismutase activity in the blood, liver and kidney and lowered malondialdehyde level. The results indicate that L. ingluviei ADK10 has potential free radical scavenging activity for the treatment of oxidative stress related disease.

International journal of pharma and bio sciences, 2016

Hyperlipidimia is a medical condition where there is an elevation of lipids, or fats, in the bloo... more Hyperlipidimia is a medical condition where there is an elevation of lipids, or fats, in the blood. The purpose of the study was to evaluate the hypolipidemic effect of sesame seed on experimentally induced hyperlipidemic albino rats. The study was carried out with thirty Wistar stain albino male rats and the groups were Group I was denoted as control, they were provided normal food, water with 1g multi-vitamin for 60 days. Group II was denoted as hyperlipidemic, from 1 st to 14 th day they were provided normal food, water and on the 15 th to 60 th day (45 days) they were provided normal food with 3.7 ml coconut oil with 1mg/kg cholesterol per rat, 1g multi-vitamin, and 5% sucrose for induction of hyperlipidemia. Group III, Group IV, Group V were denoted as treatment I, I, III respectively and they were treated as group II rats and fed orally sesame seed extract at the dose of 25, 50 and 75 mg/kg body weight/day/rat orally for 45 days respectively. The result of the present study sh...

Now days, herbal medicines are widely used to treat, manage and cure the kidney diseases as well ... more Now days, herbal medicines are widely used to treat, manage and cure the kidney diseases as well as reduce uremia, acute nephritis & nephritic syndrome. The present study was to evaluate the antiuremic and antioxidative effect of Asparagus racemosus (AR) on acetaminophen induced uremic male rats. The study was designed with 36 male albino rats which were randomly divided into 6 groups. Group I animals were provided normal food and water ad libitum, Group II, III, IV, V and VI received acetaminophen intraperitoneally at the dose of 500 mg/kg body weight/day for 10 days. Methanol, aqueous, hydromethanol, and hexane extract of AR at the dose of 500mg/kg body weight/day were fed orally on the 11 th day and continue for next 15 days to group III, IV, V and VI respectively. After 25 days, group-II animals showed significantly increased (p<0.05) plasma urea, creatinine, sodium. Elevation of lipid peroxidation was noted by measuring Malondialdehyde level in both plasma and kidney tissues...

The gastrointestinal tract of the human and other mammals is populated by a vast and diverse grou... more The gastrointestinal tract of the human and other mammals is populated by a vast and diverse group of microbes in a composite manner (Frank and Pace, 2008; Ito, 2005). Bacteria are the major population of the alimentary tract and this native microflora is commonly designated the gut microflora (Ito, 2005). Microbial flora present in the microenvironment of the gastrointestinal tract performs several important and essential activities of the host (Straw, 1989) such as the breakdown of undigested food, metabolism of drugs, enhanced absorption of foodstuffs, synthesis of vitamins, creation of resistance against pathogenic bacteria by colonization resistance, stimulation of host ISSN: 2319-7706 Volume 3 Number 8 (2014) pp. 282-291 http://www.ijcmas.com

Heart disease and Diabetes possess the first and second leading cause of death in the world and p... more Heart disease and Diabetes possess the first and second leading cause of death in the world and people are suffering from chronic kidney disease are likely more tend to suffer from hypercholesterolemia. Alpha-lipoic acid is unsaturated fatty acids having strong anti-oxidative properties. The aim of this study was to characterize alpha-lipoic acid as a cholesterol reducer for acetaminophen induced uremic rats. The study was performed on thirty male albino rats. Plasma total cholesterol, triglyceride levels, liver enzymes assay and hematologic parameters were performed to investigate its cholesterol reducing properties. In this present study it was observed that receiving acetaminophen intraperitoneally for 10 days significantly decreased Hb level and RBC count compared with control. The present study demonstrated that cholesterol and triglycerides levels were significantly higher in acetaminophen treated group compare to control. Alpha-lipoic acid ameliorates cholesterol and triglyce...

ARTICLE INFO ABSTRACT Article history Received 12 August 2016 Accepted 3 September 2016 Uremia, a... more ARTICLE INFO ABSTRACT Article history Received 12 August 2016 Accepted 3 September 2016 Uremia, acute kidney injury (AKI), chronic kidney disease (CKD) and end-stage renal disease (ESRD) have become worldwide public health problems. There are many causes for acute renal failure which mainly includes acute tubular necrosis which occurs either due to ischemia or toxins. CKD or ESRD is a gradual decrease of kidney function in both kidneys over a period of time. These conditions increase patient morbidity and mortality risks and treatment option is only dialysis and kidney transplantation which is very much expensive and not free from side effects. So that the maximum people of our country cannot afford this therapy to treat kidney disorders. However, In absence of reliable and effective modern nephroprotective drugs and traditional medicines, different scientists in different part of the World is trying to employed for the treatment of the diseases by using complementary or alternative...

Saudi Journal of Kidney Diseases and Transplantation, 2018

Oxygen is very important to the existence of life. Oxygen deficiency, defined as hypoxia, elicits... more Oxygen is very important to the existence of life. Oxygen deficiency, defined as hypoxia, elicits adaptive responses in cells and tissues. Lower oxygen concentration can cause the alteration of renal function, affects the maintenance of a balance of the body fluids, electrolytes, pH, and blood pressure homeostasis. Impaired fluid regulation could, in addition, contribute to the precipitation of pulmonary edema and exacerbate hypoxemia which may accelerate the progression of chronic kidney disease. In this context, the present study attempted to evaluate the association of renal injury and oxidative stress at different atmospheric pressures (1829, 3657, and 5486 m). Limited fecal analysis of experimental animals was also done to evaluate the impact of hypobaric hypoxia on the composition of dominant gastrointestinal microbiota. The study was performed on 24 male Wister strain rats and divided into four groups (C, HA-I, HA-II, and HA-III), and exposure was carried out for seven days period. In hypoxic exposure rats, plasma urea, creatinine, electrolytes and malonaldehyde level elevated and catalase and superoxide dismutase level diminished significantly compared to the controls. Increase in blood uremia profile, toxicity markers, and lipid peroxidation marker enzymes indicated that hypoxia causes renal failure. Histological structures of the kidney of group HA-II and HA-III animals showed severe disorganization of glomerulus and dilation of renal tubules. These results indicate nephrotoxicity or acute renal failure can occur at hypobaric hypoxia and it also affected the gut microbial population. This alteration was observed significantly above 3000 m.

International Journal of Pharmacy and Pharmaceutical Sciences, Dec 1, 2017

The aim of this study was to evaluate the antioxidant property of the isolated phytocompounds fro... more The aim of this study was to evaluate the antioxidant property of the isolated phytocompounds from TA (Terminalia arjuna) bark and in vivo study for nephro-protective and oxidative stress reducing activity in experimentally induced albino male rats. Methods: Fractions from methanol crude TA extract were collected by column chromatography and F27, F28, F29 fractions were selected on the basis of antioxidant property by 2,2-diphenyl-1-picrylhydrazyl (DPPH) scavenging assay. The in vivo study performed by 30 albino male rats which were randomly divided into five groups: Group I (control)were taken normal food and water, Groups II (uremic) were injected acetaminophen intraperitoneally at the dose of 500 mg/kg/d for 10 d, Group III, IV and V(extract treatment) acetaminophen intraperitoneally at the dose of 500 mg/kg/d for 10 d with co-administered orally of methanol fraction F27, F28, F29 at the dose of 100 mg/kg/d for 15 d respectively. Results: After scarification of rats, the uremic marker plasma urea (80%), creatinine (85%) were elevated and antioxidant enzyme marker such as plasma SOD and catalase level were significantly increased (p<0.05)in Group IV compared to Group II. The total phenolic content of the F28 methanolic fraction was (815.48±8.11) mg gallic acid equivalent/g of extract. For isolation of available compound by 1 Conclusion: Among the three methanolic fraction of TA bark, F28 was shown best antioxidative, nephron-protective and oxidative stress reducing property. H NMR study in F28 methanol fraction of TA bark was arjunoside IV which contained olefinic proton (a pair of carbon atom linked with double bond).

Acta Biologica Szegediensis, 2018

Acetaminophen-induced renal necrosis and insufficiency occurs in patients with acetaminophen over... more Acetaminophen-induced renal necrosis and insufficiency occurs in patients with acetaminophen overdose. Renal failure is rapidly assuming epidemic proportions globally. In absence of reliable and effective nephroprotective drugs, strategies towards exploring alternative therapies for treatment of kidney diseases are essential. Asparagus racemosus is a medicinal plant used for treatment of various ailments. This research was undertaken to investigate the protective effect of ethanol fraction of A. racemosus roots extract in acetaminophen-induced uraemia and renal failure in rats. Rats were co-administered with acetaminophen injection and oral administration of A. racemosus roots extract in an attempt of protection against renal failure. Uremic biomarkers significantly decreased, and elevated levels of antioxidant enzymes were found, in the animals treated with ethanol fraction of A. racemosus when compared with acetaminophen treated uremic animals. Also, histology of kidneys showed co...

Clinical Nutrition Experimental, 2018

International Journal of Research in Ayurveda & Pharmacy, 2017

The present study is designed to investigate the comparison level of antimicrobial, antioxidant p... more The present study is designed to investigate the comparison level of antimicrobial, antioxidant properties and as well as the total phenolic content estimation of aqueous extract between dusts and leaf black tea. Antimicrobial assay as well as antioxidant activity of phyto-compounds in both types of black tea was detected by using agar well diffusion and chromatographic technique respectively. Total antioxidant activity and phenolic content were measured by using coloriemetric method. In comparison level the antimicrobial activity and screening of phytochemical compounds like saponins, phenolic compounds & tannins, flavonoids, triterpenoids were significantly (P<0.05) different in both aqueous extract of leaf black tea and dust black tea. Total phenolic contents in the leaf black tea were 130.51mg Gallic acid equivalent/g of dried aqueous extract. In conclusion the leaf black tea has potential to be used as a natural antioxidant which is attributed to the rich presence of secondary metabolites and exhibit medicinal as well as physiological activities.

International Journal of Pharmacy and Pharmaceutical Sciences, Sep 1, 2014

Journal of Biological Sciences, 2015

Acetaminophen is a commonly used analgesic and antipyretic drug, high doses of which cause hepati... more Acetaminophen is a commonly used analgesic and antipyretic drug, high doses of which cause hepatic and renal injury. In development and progression of kidney disease research, it is necessary to have a suitable common drug to induce uremia and renal failure of rats. It is also required to select the threshold doses for the said drug; a therapeutic dose and toxic dose for kidney failure using standard guidelines. An acute toxicity of acetaminophen was conducted by the limit test at a dose of 2000 mg kgG 1 b.wt., on either sex rats (n = 5) and a main test was conducted by a dose progression factor of 3.2 times as per Organization of Economic CoOperation and Development guidelines 425. Eighteen male albino rats (n = 18) were divided into three groups, group I served as control, groups II and III rats were administered 175 mg and 550 mg kgG 1 b.wt., acetaminophen intraperitoneally for 14 days, respectively. Different parameters were considered to analyze renal failure. Urea, creatinine, GOT, GPT and MDA levels were increased significantly (p<0.05) in group III, compared to groups I and II. Antioxidant enzymes like SOD, catalase and GSH level were decreased significantly (p<0.05) in group III rats, compared to group I and II rats. Increase in blood uremia profile indicated that the higher dose of acetaminophen causes uremia. Increase in the toxicity markers and lipid peroxidation marker enzymes indicate the nephrotoxicity. Histological structures of kidney of group III animals showed a severe disorganization of glomerulus and dilation of renal tubules. These results indicate that intraperitoneal injection of acetaminophen at high dose causes nephrotoxicity and renal cellular damage to experimental rats.

Indian journal of experimental biology, 2013

The total antioxidative activity of L. ingluviei ADK10 isolated from chicken intestine intact cel... more The total antioxidative activity of L. ingluviei ADK10 isolated from chicken intestine intact cells and cell free culture supernatant (CFCS) was 54- 67.95%. The ability to scavenge a,a-Diphenyl-b-Picrylhydrazyl free radical ranged from 71 and 64% in intact cells and CFCS respectively. Total reducing activity of bacteria was equivalent to 290 microM/L of cysteine. Reducing glutathione activity was equivalent to 93.95 microg/mL. Oral administration of the strain at a dose of 10(9) cfu/kg body weight to acetaminophen induced oxidative stress in rats increased catalase, glutathione and superoxide dismutase activity in the blood, liver and kidney and lowered malondialdehyde level. The results indicate that L. ingluviei ADK10 has potential free radical scavenging activity for the treatment of oxidative stress related disease.

Asian Pacific journal of tropical biomedicine, 2013

To investigate phytochemical screening, antimicrobial activity and qualitative thin layer chromat... more To investigate phytochemical screening, antimicrobial activity and qualitative thin layer chromatographic separation of flavonoid components, antioxidant activity and total flavonoid compound of Terminalia arjuna. For phytochemical screening, some common and available standard tests were done. Antimicrobial bioassay was done through agar well diffusion method. Detection of antioxidant activity and flavonoid compounds were done through thin layer chromatography. Total antioxidant activity was measured by 2, 2-diphenyl-1-picrylhydrazyl (DPPH) in colorimetric method. Aluminum chloride colorimetric method was used for total flavonoid determination. Phytochemical screening showed the active compounds presence in high concentration, such as phytosterol, lactones, flavonoids, phenolic compounds and tannins and glycosides. The antimicrobial activity of extract showed that greater inhibition zone against Gram negative bacteria than Gram positive bacteria. This methanolic extract showed a pro...

Biomedicine & Preventive Nutrition, 2014

ABSTRACT