MARCIA SALDANHA KUBRUSLY - Academia.edu (original) (raw)
Papers by MARCIA SALDANHA KUBRUSLY
International Journal of Molecular Sciences, Jun 17, 2023
This article is an open access article distributed under the terms and conditions of the Creative... more This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY
Revista do Hospital das Clinicas da Faculdade de Medicina da Universidade de Sao Paulo, 1993
Frontiers in Endocrinology, Nov 27, 2020
Non-alcoholic fatty liver disease (NAFLD) is one of the most common forms of liver disease, which... more Non-alcoholic fatty liver disease (NAFLD) is one of the most common forms of liver disease, which is associated with several etiological factors, including stress and dysfunction in oxidative metabolism. However, studies showed that aerobic exercise training (AET) can combat the oxidative stress (OS) and improves mitochondrial functionality in the NAFLD. To test the hypothesis that AET improves oxidative metabolism and antioxidant defense in the liver of ob/ob mice. Male ob/ob mice with eight weeks old were separated into two groups: the sedentary group (S), n=7, and the trained group (T), n=7. The T mice were submitted to an 8-week protocol of AET at 60% of the maximum velocity achieved in the running capacity test. Before AET, no difference was observed in running test between the groups (S=10.4 ± 0.7 min vs. T= 13 ± 0.47 min). However, after AET, the running capacity was increased in the T group (12.8 ± 0.87 min) compared to the S group (7.2 ± 0.63 min). In skeletal muscle, the T group (26.91 ± 1.12 U/ mg of protein) showed higher citrate synthase activity compared with the S group (19.28 ± 0.88 U/mg of protein) (p =0.006). In the analysis of BW evolution, significant reductions were seen in the T group as of the fourth week when compared to the S group. In addition, food intake was not significant different between the groups. Significant increases were observed in the activity of enzymes citrate synthase (p=0.004) and b-HAD (p=0.01) as well as in PGC-1a gene expression (p=0.002) in the liver of T group. The levels of TBARs and carbonyls, as well as SOD, CAT and GST were not different between the groups. However, in the nonenzymatic antioxidant system, we found that the T group had
Pancreas, Nov 1, 2008
Background: Myelodysplastic syndromes (MDS) are a group of clonal hematological disorders charact... more Background: Myelodysplastic syndromes (MDS) are a group of clonal hematological disorders characterized by ineffective hematopoiesis with morphological evidence of marrow cell dysplasia resulting in peripheral blood cytopenia. Microarray technology has permitted a refined high-throughput mapping of the transcriptional activity in the human genome. Non-coding RNAs (ncRNAs) transcribed from intronic regions of genes are involved in a number of processes related to post-transcriptional control of gene expression, and in the regulation of exon-skipping and intron retention. Characterization of ncRNAs in progenitor cells and stromal cells of MDS patients could be strategic for understanding gene expression regulation in this disease. Methods: In this study, gene expression profiles of CD34 + cells of 4 patients with MDS of refractory anemia with ringed sideroblasts (RARS) subgroup and stromal cells of 3 patients with MDS-RARS were compared with healthy individuals using 44 k combined intron-exon oligoarrays, which included probes for exons of protein-coding genes, and for noncoding RNAs transcribed from intronic regions in either the sense or antisense strands. Real-time RT-PCR was performed to confirm the expression levels of selected transcripts. Results: In CD34 + cells of MDS-RARS patients, 216 genes were significantly differentially expressed (q-value ≤ 0.01) in comparison to healthy individuals, of which 65 (30%) were non-coding transcripts. In stromal cells of MDS-RARS, 12 genes were significantly differentially expressed (q-value ≤ 0.05) in comparison to healthy individuals, of which 3 (25%) were non-coding transcripts. Conclusions: These results demonstrated, for the first time, the differential ncRNA expression profile between MDS-RARS and healthy individuals, in CD34 + cells and stromal cells, suggesting that ncRNAs may play an important role during the development of myelodysplastic syndromes.
Gastroenterology, May 1, 2011
Vascular endothelium is a major target for the inflammatory damage that occurs with multiple orga... more Vascular endothelium is a major target for the inflammatory damage that occurs with multiple organ dysfunction associated with sepsis and other trauma. The growing appreciation of endothelium as a target of inflammation has obscured the importance of these cells as a source of inflammatory mediators. In the following study we evaluated the ability of tumor necrosis factor-alpha (TNF) to induce the synthesis of complement component C3 in human umbilical vein endothelial cells (HUVEC) and whether pentoxifylline (PTX) could reduce C3 expression. Confluent monolayers of HUVEC were treated with increasing concentrations of TNF with and without two concentrations of PTX. Concentrations of C3 were determined every 48 h for 144 h in cellular supernatants and C3 mRNA was amplified using RT-PCR. TNF increased C3 release from HUVEC in a concentration dependent manner. PTX added at the same time as TNF significantly reduced C3 release at the 96 h time point. Consistent with data on C3 release PTX inhibited the increased C3 mRNA expression associated with TNF treatment. TNF increases C3 synthesis and release from endothelial cells which were inhibited by clinical concentrations of PTX. This data further supports the potential benefit of PTX in multiple organ dysfunction and other inflammatory processes involving the endothelium by inhibiting one of the major mediators of vascular damage.
Liver Transplantation, 2008
Nutraceuticals are bioactive compounds present in foods, utilized to ameliorate health, prevent d... more Nutraceuticals are bioactive compounds present in foods, utilized to ameliorate health, prevent diseases, and support the proper functioning of the human body. They have gained attention due to their ability to hit multi-targets and act as antioxidants, anti-inflammatory agents, and modulators of immune response and cell death. Therefore, nutraceuticals are being studied to prevent and treat liver ischemia–reperfusion injury (IRI). This study evaluated the effect of a nutraceutical solution formed by resveratrol, quercetin, omega-3 fatty acid, selenium, ginger, avocado, leucine, and niacin on liver IRI. IRI was performed with 60 min of ischemia and 4 hours of reperfusion in male Wistar rats. Afterward, the animals were euthanized to study hepatocellular injury, cyto-kines, oxidative stress, gene expression of apoptosis-related genes, TNF-alpha and Caspase 3 proteins, and histology. Our results show that the nutraceutical solution was able to decrease apoptosis and histologic injury....
Cadernos de Agroecologia, May 19, 2020
Cadernos de Agroecologia, Aug 23, 2018
Revista do Hospital das Clinicas. Faculdade de Medicina da Universidade de Sao Paulo, 1996
Aesthetic Plastic Surgery, 2020
Background Smoking causes a threefold increase in the risk of surgical complications in flaps. Hy... more Background Smoking causes a threefold increase in the risk of surgical complications in flaps. Hyperbaric oxygen therapy (HBOT) increases the viability of chronic wounds. However, there are few studies concerning the effects of HBOT on surgical flaps in patients who smoke. This study aimed to analyze the effect of HBOT on the viability of cutaneous flaps in tobacco-exposed rats. Methods Twenty Wistar rats were exposed to tobacco smoke for two months. Following this period, all animals underwent a dorsal cutaneous flap (3 9 10 cm) surgery and were divided into two groups: control (n = 10) and HBOT (n = 10). HBOT was performed in seven daily sessions (2 ATA, 90 min). After seven days, the animals were euthanized. The outcomes were total area, viable area, viable area/total area rate, analysis of dermal appendages and angiogenesis (hematoxylin-eosin), and gene expression analysis of iNOS and VEGF-a biomarkers. Results The HBOT group showed an increase in viable area compared with the control group (84% versus 47%, p = 0.009, respectively). The HBOT group also showed an increase in appendage units (1.69 ± 0.54 versus 1.87 ± 0.58, p = 0.04) and angiogenesis density (1.29 ± 0.45 versus 1.82 ± 0.64, p \ 0.001) compared to the control group. There was a difference between the control and HBOT groups in iNOS levels (0.926 ± 1.4 versus 0.04 ± 0.1 p = 0.002, respectively). However, this study did not show a difference between the groups concerning the gene expression of VEGF-a. Conclusion The use of hyperbaric oxygen therapy increased the viability of cutaneous flaps in tobacco-exposed rats and decreased iNOS mRNA levels; however, it did not change VEGF-a levels.
HPB, 2000
Pulmonary insufficiency is a frequent complication of acute pancreatitis (AP). Intravenous admini... more Pulmonary insufficiency is a frequent complication of acute pancreatitis (AP). Intravenous administration of cerulein in small doses decreases the pancreatic enzyme content and reduces the mortality rate of experimental AP.We stud ied the influence of pancreatic enzyme reduction on the pulmonary lesions of experimental AP. Methods Pulmonary oedema was evaluated by microscopic lung morphometry in rats 24 hours after the induction of sodium taurocholate (Na+ Tachol) Ap, with and without a previous intravenous injection of cerulein. Experimental groups were as follows : Group I: No cerulein infusion and AP induced by Na+Tachol at 2.5% (Group IA) and at 4% (Group IS) ; Group II : Previous cerulein infusion and AP induced by Na+Tachol at 2.5% (Group IIA) and at 4% (Group liB) ; Group III : Cerulein infusion withoutAP.
Gastroenterology, 2017
of 59 consecutive patients with IPMN diagnosed histologically after pancreatectomy between 2006 a... more of 59 consecutive patients with IPMN diagnosed histologically after pancreatectomy between 2006 and 2016 were retrospectively reviewed. Endoscopic ultrasonography was performed in 50 of the 59 patients. Malignant IPMN included high-grade dysplasia as well as invasive carcinoma. Results: Twenty patients had malignant IPMN, while 39 had benign IPMN. Thirty-five patients had HRS, and 18 (51%) of the patients with HRS had malignant IPMN. The sensitivity, specificity and positive and negative predictive values of HRS for malignant IPMN were 90%, 56%, 51% and 92%, respectively. NLRs tended to be higher in patients with malignant IPMN (median, 2.23; range, 1.05-10.63)) than in those with benign IPMN (median, 2.04; range, 0.58-5.68; P = 0.15). Mural nodules were detected by endoscopic ultrasonography in 40 patients. Mural nodule heights were significantly greater in patients with malignant IPMN (median, 16 mm; range, 2-39 mm) than in those with benign IPMN (median, 8 mm; range, 2-30 mm; P <0.01). The optimal cut off values for the NLR and the mural nodule height were calculated by receiver operating characteristic curve analysis to be 3.60 and 11 mm, respectively. The sensitivity, specificity and positive and negative predictive values of NLR ≥ 3.60 for predicting malignant IPMN were 40%, 92%, 72% and 75%, respectively, and those of the mural nodule height ≥ 11 mm were 73%, 76%, 65% and 83%, respectively. Univariate analysis revealed that main duct type IPMN (P <0.01), presence of enhanced solid component (P <0.01), abrupt change in caliber of main pancreatic duct (P <0.01), NLR ≥ 3.60 (P <0.01) and mural nodule height ≥ 11 mm (P <0.01) were significant preoperative factors for predicting malignant IPMN. Multivariate analysis demonstrated that presence of enhanced solid component (odds ratio, 8.20; 95% confidence interval, 1.14-90.97) and mural nodule height ≥ 11 mm (odds ratio, 6.51; 95% confidence interval, 1.4-59.36) were independent factors. Conclusion: The NLR and mural nodule height might be useful markers for predicting which IPMNs must be surgically resected. The NLR should be added to the list of worrisome features, and mural nodule height should be added to HRS criteria. Univariate and multivariate analysis for predicting malignant intraductal papillary mucinous neoplasms CI, confidence interval; NA, not available; IPMN, intraductal papillary mucinous neoplasm; MPD, main pancreatic duct; NLR, neutrophil-to-lymphocyte ratio Su1284
Gastroenterology, 2017
Background: Peripancreatic drain that is placed after distal pancreatectomy sometimes migrates an... more Background: Peripancreatic drain that is placed after distal pancreatectomy sometimes migrates and becomes ineffective, postoperatively. We devised a new drainage method with fixation of the tip of a peripancreatic drain. Methods: This retrospective study was done on 84 consecutive patients who underwent distal pancreatectomy followed by peripancreatic drainage (silicone duple drain) with (n=31) or without (n=53) fixation. In the drain fixation group, the tip of the drain was fixed to the retroperitoneal tissue close to the pancreatic stump, with a loose loop of an absorbable suture (Figure 1). Results: The fixed drain remained in place postoperatively and was easily removed when the drainage became unnecessary. Pancreatic fistula (ISGPF, Grade B/C) developed in four patients (13%) with and 11 (21%) without drain fixation, the incidence being not different. None with and seven patients (13%) without fixation required additional drainage (interventional or surgical) for pancreatic fistula, the difference being significant. Time to resolution of pancreatic fistula tended to be shorter after drain fixation (20 days) than after non-fixation (31 days). Conclusion: Fixation of the tip of a peripancreatic drain is a simple but useful technique for effective drainage after distal pancreatectomy.
International Journal of Molecular Sciences, Jun 17, 2023
This article is an open access article distributed under the terms and conditions of the Creative... more This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY
Revista do Hospital das Clinicas da Faculdade de Medicina da Universidade de Sao Paulo, 1993
Frontiers in Endocrinology, Nov 27, 2020
Non-alcoholic fatty liver disease (NAFLD) is one of the most common forms of liver disease, which... more Non-alcoholic fatty liver disease (NAFLD) is one of the most common forms of liver disease, which is associated with several etiological factors, including stress and dysfunction in oxidative metabolism. However, studies showed that aerobic exercise training (AET) can combat the oxidative stress (OS) and improves mitochondrial functionality in the NAFLD. To test the hypothesis that AET improves oxidative metabolism and antioxidant defense in the liver of ob/ob mice. Male ob/ob mice with eight weeks old were separated into two groups: the sedentary group (S), n=7, and the trained group (T), n=7. The T mice were submitted to an 8-week protocol of AET at 60% of the maximum velocity achieved in the running capacity test. Before AET, no difference was observed in running test between the groups (S=10.4 ± 0.7 min vs. T= 13 ± 0.47 min). However, after AET, the running capacity was increased in the T group (12.8 ± 0.87 min) compared to the S group (7.2 ± 0.63 min). In skeletal muscle, the T group (26.91 ± 1.12 U/ mg of protein) showed higher citrate synthase activity compared with the S group (19.28 ± 0.88 U/mg of protein) (p =0.006). In the analysis of BW evolution, significant reductions were seen in the T group as of the fourth week when compared to the S group. In addition, food intake was not significant different between the groups. Significant increases were observed in the activity of enzymes citrate synthase (p=0.004) and b-HAD (p=0.01) as well as in PGC-1a gene expression (p=0.002) in the liver of T group. The levels of TBARs and carbonyls, as well as SOD, CAT and GST were not different between the groups. However, in the nonenzymatic antioxidant system, we found that the T group had
Pancreas, Nov 1, 2008
Background: Myelodysplastic syndromes (MDS) are a group of clonal hematological disorders charact... more Background: Myelodysplastic syndromes (MDS) are a group of clonal hematological disorders characterized by ineffective hematopoiesis with morphological evidence of marrow cell dysplasia resulting in peripheral blood cytopenia. Microarray technology has permitted a refined high-throughput mapping of the transcriptional activity in the human genome. Non-coding RNAs (ncRNAs) transcribed from intronic regions of genes are involved in a number of processes related to post-transcriptional control of gene expression, and in the regulation of exon-skipping and intron retention. Characterization of ncRNAs in progenitor cells and stromal cells of MDS patients could be strategic for understanding gene expression regulation in this disease. Methods: In this study, gene expression profiles of CD34 + cells of 4 patients with MDS of refractory anemia with ringed sideroblasts (RARS) subgroup and stromal cells of 3 patients with MDS-RARS were compared with healthy individuals using 44 k combined intron-exon oligoarrays, which included probes for exons of protein-coding genes, and for noncoding RNAs transcribed from intronic regions in either the sense or antisense strands. Real-time RT-PCR was performed to confirm the expression levels of selected transcripts. Results: In CD34 + cells of MDS-RARS patients, 216 genes were significantly differentially expressed (q-value ≤ 0.01) in comparison to healthy individuals, of which 65 (30%) were non-coding transcripts. In stromal cells of MDS-RARS, 12 genes were significantly differentially expressed (q-value ≤ 0.05) in comparison to healthy individuals, of which 3 (25%) were non-coding transcripts. Conclusions: These results demonstrated, for the first time, the differential ncRNA expression profile between MDS-RARS and healthy individuals, in CD34 + cells and stromal cells, suggesting that ncRNAs may play an important role during the development of myelodysplastic syndromes.
Gastroenterology, May 1, 2011
Vascular endothelium is a major target for the inflammatory damage that occurs with multiple orga... more Vascular endothelium is a major target for the inflammatory damage that occurs with multiple organ dysfunction associated with sepsis and other trauma. The growing appreciation of endothelium as a target of inflammation has obscured the importance of these cells as a source of inflammatory mediators. In the following study we evaluated the ability of tumor necrosis factor-alpha (TNF) to induce the synthesis of complement component C3 in human umbilical vein endothelial cells (HUVEC) and whether pentoxifylline (PTX) could reduce C3 expression. Confluent monolayers of HUVEC were treated with increasing concentrations of TNF with and without two concentrations of PTX. Concentrations of C3 were determined every 48 h for 144 h in cellular supernatants and C3 mRNA was amplified using RT-PCR. TNF increased C3 release from HUVEC in a concentration dependent manner. PTX added at the same time as TNF significantly reduced C3 release at the 96 h time point. Consistent with data on C3 release PTX inhibited the increased C3 mRNA expression associated with TNF treatment. TNF increases C3 synthesis and release from endothelial cells which were inhibited by clinical concentrations of PTX. This data further supports the potential benefit of PTX in multiple organ dysfunction and other inflammatory processes involving the endothelium by inhibiting one of the major mediators of vascular damage.
Liver Transplantation, 2008
Nutraceuticals are bioactive compounds present in foods, utilized to ameliorate health, prevent d... more Nutraceuticals are bioactive compounds present in foods, utilized to ameliorate health, prevent diseases, and support the proper functioning of the human body. They have gained attention due to their ability to hit multi-targets and act as antioxidants, anti-inflammatory agents, and modulators of immune response and cell death. Therefore, nutraceuticals are being studied to prevent and treat liver ischemia–reperfusion injury (IRI). This study evaluated the effect of a nutraceutical solution formed by resveratrol, quercetin, omega-3 fatty acid, selenium, ginger, avocado, leucine, and niacin on liver IRI. IRI was performed with 60 min of ischemia and 4 hours of reperfusion in male Wistar rats. Afterward, the animals were euthanized to study hepatocellular injury, cyto-kines, oxidative stress, gene expression of apoptosis-related genes, TNF-alpha and Caspase 3 proteins, and histology. Our results show that the nutraceutical solution was able to decrease apoptosis and histologic injury....
Cadernos de Agroecologia, May 19, 2020
Cadernos de Agroecologia, Aug 23, 2018
Revista do Hospital das Clinicas. Faculdade de Medicina da Universidade de Sao Paulo, 1996
Aesthetic Plastic Surgery, 2020
Background Smoking causes a threefold increase in the risk of surgical complications in flaps. Hy... more Background Smoking causes a threefold increase in the risk of surgical complications in flaps. Hyperbaric oxygen therapy (HBOT) increases the viability of chronic wounds. However, there are few studies concerning the effects of HBOT on surgical flaps in patients who smoke. This study aimed to analyze the effect of HBOT on the viability of cutaneous flaps in tobacco-exposed rats. Methods Twenty Wistar rats were exposed to tobacco smoke for two months. Following this period, all animals underwent a dorsal cutaneous flap (3 9 10 cm) surgery and were divided into two groups: control (n = 10) and HBOT (n = 10). HBOT was performed in seven daily sessions (2 ATA, 90 min). After seven days, the animals were euthanized. The outcomes were total area, viable area, viable area/total area rate, analysis of dermal appendages and angiogenesis (hematoxylin-eosin), and gene expression analysis of iNOS and VEGF-a biomarkers. Results The HBOT group showed an increase in viable area compared with the control group (84% versus 47%, p = 0.009, respectively). The HBOT group also showed an increase in appendage units (1.69 ± 0.54 versus 1.87 ± 0.58, p = 0.04) and angiogenesis density (1.29 ± 0.45 versus 1.82 ± 0.64, p \ 0.001) compared to the control group. There was a difference between the control and HBOT groups in iNOS levels (0.926 ± 1.4 versus 0.04 ± 0.1 p = 0.002, respectively). However, this study did not show a difference between the groups concerning the gene expression of VEGF-a. Conclusion The use of hyperbaric oxygen therapy increased the viability of cutaneous flaps in tobacco-exposed rats and decreased iNOS mRNA levels; however, it did not change VEGF-a levels.
HPB, 2000
Pulmonary insufficiency is a frequent complication of acute pancreatitis (AP). Intravenous admini... more Pulmonary insufficiency is a frequent complication of acute pancreatitis (AP). Intravenous administration of cerulein in small doses decreases the pancreatic enzyme content and reduces the mortality rate of experimental AP.We stud ied the influence of pancreatic enzyme reduction on the pulmonary lesions of experimental AP. Methods Pulmonary oedema was evaluated by microscopic lung morphometry in rats 24 hours after the induction of sodium taurocholate (Na+ Tachol) Ap, with and without a previous intravenous injection of cerulein. Experimental groups were as follows : Group I: No cerulein infusion and AP induced by Na+Tachol at 2.5% (Group IA) and at 4% (Group IS) ; Group II : Previous cerulein infusion and AP induced by Na+Tachol at 2.5% (Group IIA) and at 4% (Group liB) ; Group III : Cerulein infusion withoutAP.
Gastroenterology, 2017
of 59 consecutive patients with IPMN diagnosed histologically after pancreatectomy between 2006 a... more of 59 consecutive patients with IPMN diagnosed histologically after pancreatectomy between 2006 and 2016 were retrospectively reviewed. Endoscopic ultrasonography was performed in 50 of the 59 patients. Malignant IPMN included high-grade dysplasia as well as invasive carcinoma. Results: Twenty patients had malignant IPMN, while 39 had benign IPMN. Thirty-five patients had HRS, and 18 (51%) of the patients with HRS had malignant IPMN. The sensitivity, specificity and positive and negative predictive values of HRS for malignant IPMN were 90%, 56%, 51% and 92%, respectively. NLRs tended to be higher in patients with malignant IPMN (median, 2.23; range, 1.05-10.63)) than in those with benign IPMN (median, 2.04; range, 0.58-5.68; P = 0.15). Mural nodules were detected by endoscopic ultrasonography in 40 patients. Mural nodule heights were significantly greater in patients with malignant IPMN (median, 16 mm; range, 2-39 mm) than in those with benign IPMN (median, 8 mm; range, 2-30 mm; P <0.01). The optimal cut off values for the NLR and the mural nodule height were calculated by receiver operating characteristic curve analysis to be 3.60 and 11 mm, respectively. The sensitivity, specificity and positive and negative predictive values of NLR ≥ 3.60 for predicting malignant IPMN were 40%, 92%, 72% and 75%, respectively, and those of the mural nodule height ≥ 11 mm were 73%, 76%, 65% and 83%, respectively. Univariate analysis revealed that main duct type IPMN (P <0.01), presence of enhanced solid component (P <0.01), abrupt change in caliber of main pancreatic duct (P <0.01), NLR ≥ 3.60 (P <0.01) and mural nodule height ≥ 11 mm (P <0.01) were significant preoperative factors for predicting malignant IPMN. Multivariate analysis demonstrated that presence of enhanced solid component (odds ratio, 8.20; 95% confidence interval, 1.14-90.97) and mural nodule height ≥ 11 mm (odds ratio, 6.51; 95% confidence interval, 1.4-59.36) were independent factors. Conclusion: The NLR and mural nodule height might be useful markers for predicting which IPMNs must be surgically resected. The NLR should be added to the list of worrisome features, and mural nodule height should be added to HRS criteria. Univariate and multivariate analysis for predicting malignant intraductal papillary mucinous neoplasms CI, confidence interval; NA, not available; IPMN, intraductal papillary mucinous neoplasm; MPD, main pancreatic duct; NLR, neutrophil-to-lymphocyte ratio Su1284
Gastroenterology, 2017
Background: Peripancreatic drain that is placed after distal pancreatectomy sometimes migrates an... more Background: Peripancreatic drain that is placed after distal pancreatectomy sometimes migrates and becomes ineffective, postoperatively. We devised a new drainage method with fixation of the tip of a peripancreatic drain. Methods: This retrospective study was done on 84 consecutive patients who underwent distal pancreatectomy followed by peripancreatic drainage (silicone duple drain) with (n=31) or without (n=53) fixation. In the drain fixation group, the tip of the drain was fixed to the retroperitoneal tissue close to the pancreatic stump, with a loose loop of an absorbable suture (Figure 1). Results: The fixed drain remained in place postoperatively and was easily removed when the drainage became unnecessary. Pancreatic fistula (ISGPF, Grade B/C) developed in four patients (13%) with and 11 (21%) without drain fixation, the incidence being not different. None with and seven patients (13%) without fixation required additional drainage (interventional or surgical) for pancreatic fistula, the difference being significant. Time to resolution of pancreatic fistula tended to be shorter after drain fixation (20 days) than after non-fixation (31 days). Conclusion: Fixation of the tip of a peripancreatic drain is a simple but useful technique for effective drainage after distal pancreatectomy.