M. Alofs - Academia.edu (original) (raw)

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Papers by M. Alofs

Research paper thumbnail of Fluorescence in situ hybridization and single nucleotide polymorphism of a new case with inv dup del(8p)

Genetic counseling (Geneva, Switzerland), 2009

Fluorescence In Situ Hybridization and single nucleotide polymorphism of a new case with inv dup ... more Fluorescence In Situ Hybridization and single nucleotide polymorphism of a new case with inv dup del(8p): Inverted duplication deletion of 8p [inv dup del(8p)] is a complex chromosome rearrangement leading among others to deletion of the chromosome region distal to the duplication in 8p. A new case with an inverted duplication deletion of 8p and the results of SNP-array analysis and fluorescence in situ hybridization (FISH) are reported here. Our results are in concordance with earlier reported inv dup del(8p) cases.

Research paper thumbnail of A patient with a de novo 11q24.2-->qter deletion

Genetic counseling (Geneva, Switzerland), 2004

In the group of patients with terminal 11q deletion reported up to now. Jacobson syndrome has bee... more In the group of patients with terminal 11q deletion reported up to now. Jacobson syndrome has been delineated as a distinct clinical entity. In the present report we describe the clinical findings in a 3-year old girl with de novo deletion 11q24.2-->11qter, and compare the findings with Jacobson syndrome.

Research paper thumbnail of Intrachromosomal insertion translocation resulting in duplication of chromosome band Yq11.2 in two fertile brothers

American Journal of Medical Genetics, 2003

Chromosome analysis in a couple referred because of two spontaneous abortions showed a normal 46,... more Chromosome analysis in a couple referred because of two spontaneous abortions showed a normal 46,XX karyotype in the 28-year-old female and an aberrant Y chromosome with an enlarged short arm in the 30-year-old male. Subsequent chromosome analysis showed that his 33-year-old brother was carrier of the same Y chromosome aberration. Further characterization of the aberrant Y chromosome with FISH using probes specific for chromosome bands Yp11.32, Yq11.2, the centromere and the subtelomeric region of the p-arm of the Y chromosome showed that chromosome band Yq11.2 was duplicated and inserted in the p-arm of the Y chromosome. Combining the results of the analysis of GTG-banded chromosomes and of the FISH analysis we conclude that both patients have a 46,X,ins dup(Y)(pter --> p11.23::q12 --> q11.1::p11.23 -->) karyotype. The clinical and cytogenetical findings are reported and discussed.

Research paper thumbnail of Mosaic telomeric (2;14) association in a child with motor delay

American Journal of Medical Genetics, 2000

In a 6-year-old girl referred because of mild motor delay and hyperextensible joints, chromosome ... more In a 6-year-old girl referred because of mild motor delay and hyperextensible joints, chromosome analysis disclosed a derivative chromosome consisting of end-to-end fusion of chromosomes 2 and 14. Two cell lines existed in which this telomere association was present, one with a 45,XX,tas(2;14)(q37;p11) karyotype and one with a 45,XX,tas(2;14) (q37;q32) karyotype. The cell line with the telomeric fusion of 2q and 14p was present in 90% of the cells; a telomeric fusion of 2q and 14q was seen in the remaining 10% of the cells. In both association complexes, only the centromere of chromosome 14 was active. Fluorescence in situ hybridization with telomere and subtelomere probes disclosed no deletion of chromosomal material. Microsatellite analysis showed that the patient had a normal biparental contribution of chromosomes 14.

Research paper thumbnail of De novo ?pure? partial trisomy (6)(p22.1?pter) in a chromosome 15 with an enlarged satellite, identified by microdissection

American Journal of Medical Genetics, 2001

Research paper thumbnail of Fluorescence in situ hybridization and single nucleotide polymorphism of a new case with inv dup del(8p)

Genetic counseling (Geneva, Switzerland), 2009

Fluorescence In Situ Hybridization and single nucleotide polymorphism of a new case with inv dup ... more Fluorescence In Situ Hybridization and single nucleotide polymorphism of a new case with inv dup del(8p): Inverted duplication deletion of 8p [inv dup del(8p)] is a complex chromosome rearrangement leading among others to deletion of the chromosome region distal to the duplication in 8p. A new case with an inverted duplication deletion of 8p and the results of SNP-array analysis and fluorescence in situ hybridization (FISH) are reported here. Our results are in concordance with earlier reported inv dup del(8p) cases.

Research paper thumbnail of A patient with a de novo 11q24.2-->qter deletion

Genetic counseling (Geneva, Switzerland), 2004

In the group of patients with terminal 11q deletion reported up to now. Jacobson syndrome has bee... more In the group of patients with terminal 11q deletion reported up to now. Jacobson syndrome has been delineated as a distinct clinical entity. In the present report we describe the clinical findings in a 3-year old girl with de novo deletion 11q24.2-->11qter, and compare the findings with Jacobson syndrome.

Research paper thumbnail of Intrachromosomal insertion translocation resulting in duplication of chromosome band Yq11.2 in two fertile brothers

American Journal of Medical Genetics, 2003

Chromosome analysis in a couple referred because of two spontaneous abortions showed a normal 46,... more Chromosome analysis in a couple referred because of two spontaneous abortions showed a normal 46,XX karyotype in the 28-year-old female and an aberrant Y chromosome with an enlarged short arm in the 30-year-old male. Subsequent chromosome analysis showed that his 33-year-old brother was carrier of the same Y chromosome aberration. Further characterization of the aberrant Y chromosome with FISH using probes specific for chromosome bands Yp11.32, Yq11.2, the centromere and the subtelomeric region of the p-arm of the Y chromosome showed that chromosome band Yq11.2 was duplicated and inserted in the p-arm of the Y chromosome. Combining the results of the analysis of GTG-banded chromosomes and of the FISH analysis we conclude that both patients have a 46,X,ins dup(Y)(pter --> p11.23::q12 --> q11.1::p11.23 -->) karyotype. The clinical and cytogenetical findings are reported and discussed.

Research paper thumbnail of Mosaic telomeric (2;14) association in a child with motor delay

American Journal of Medical Genetics, 2000

In a 6-year-old girl referred because of mild motor delay and hyperextensible joints, chromosome ... more In a 6-year-old girl referred because of mild motor delay and hyperextensible joints, chromosome analysis disclosed a derivative chromosome consisting of end-to-end fusion of chromosomes 2 and 14. Two cell lines existed in which this telomere association was present, one with a 45,XX,tas(2;14)(q37;p11) karyotype and one with a 45,XX,tas(2;14) (q37;q32) karyotype. The cell line with the telomeric fusion of 2q and 14p was present in 90% of the cells; a telomeric fusion of 2q and 14q was seen in the remaining 10% of the cells. In both association complexes, only the centromere of chromosome 14 was active. Fluorescence in situ hybridization with telomere and subtelomere probes disclosed no deletion of chromosomal material. Microsatellite analysis showed that the patient had a normal biparental contribution of chromosomes 14.

Research paper thumbnail of De novo ?pure? partial trisomy (6)(p22.1?pter) in a chromosome 15 with an enlarged satellite, identified by microdissection

American Journal of Medical Genetics, 2001

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