MB Urrutia - Academia.edu (original) (raw)

Papers by MB Urrutia

Les composantes physiologiques du bilan energetique ont ete determinees sur des Coques alimentees... more Les composantes physiologiques du bilan energetique ont ete determinees sur des Coques alimentees avec du seston naturel du Bassin de Marennes-Oleron (S.O. France). Ces determinations ont ete realisees a differents moments du cycle des marees de vives eaux et de mortes eaux. La variabilite nutritionelle provenant des fluctuations de l'abondance des particules en suspension est liee au flux de la maree et s'echelonne de 10 a 170 mg de matiere particulaire totale (MTP) par litre. Les variations de la fraction organique du seston peuvent etre expliquees pour 73 % par les fluctuations de la concentration en particules associees au cycle de la maree, qui comporte la remise en suspension du substrat, pauvre en contenu organique. La variabilite restante n'a pu etre expliquee par les fluctuations d'abondance du phytoplancton, car elle apparait faiblement correlee avec les concentrations des chlorophylles et/ou des phaeopigments du seston. Les equations derivees des mesures p...

Scientific Reports

We tested the hypothesis that environmental trophic conditions prominent during the growing perio... more We tested the hypothesis that environmental trophic conditions prominent during the growing period (nurture conditions) can modify the differing physiological profiles between fast (F)- and slow (S)-growing juveniles of the mussel Mytilus galloprovincialis. Approximately 200 individuals were fed a high organic content diet dosed below the pseudofaeces threshold (BP), whereas another 200 were fed a low organic content diet dosed above the pseudofaeces threshold (AP), forcing them to maintain a continuous production of pseudofaeces. After 3 months, F and S individuals in each rearing condition were selected and used in feeding experiments. We measured the physiological parameters of the energy balance of selected F and S mussels fed on 4 different diets and tested the effects of the rearing condition (BP vs AP) and growth condition (F vs S) upon the physiological variables. Irrespective of the rearing condition, F-mussels attained higher values of scope for growth with the four experi...

Aquaculture

Abstract The molecular basis underlying the mechanisms at the origin of growth variation in bival... more Abstract The molecular basis underlying the mechanisms at the origin of growth variation in bivalves is still poorly understood, although several genes have been described as upregulated in fast-growing individuals. In the present study, we reared mussel spat of the species Mytilus galloprovincialis under diets below the pseudofaeces threshold (BP) and above the pseudofaeces threshold (AP). After 3 months, F and S mussels from each condition were selected to obtain 4 experimental groups: FBP, SBP, FAP and SAP. We hypothesized that the nurturing conditions during the growing period would modify the molecular basis of their growth rate differences. To decipher the molecular mechanisms underlying the growth variation, the gill transcriptomes for the four mussel groups were analysed. Gene expression analysis revealed i) a low number (12) of genes differentially expressed in association with diet and ii) 117 genes differentially expressed by the fast- and slow-growing mussels. According to Biological Process GO term analysis transcriptomic differences between the F and S mussels were mainly based on the upregulation of: response to the stimulus, growth and cell activity. Regarding the KEGG terms, carbohydrate metabolism and the Krebs cycle were upregulated in F mussels, whereas biosynthetic processes were upregulated in S mussels. In accordance with their larger gill surface area and higher rates of feeding and growth, the F individuals overexpressed genes in their gill tissues, and these were involved in i) growth (insulin-like growth factors and myostatin); ii) maintenance of the structure and functioning of extracellular matrix (collagen, laminin, fibulin and decorin); iii) filtration and ciliary activity (mucin, fibrocystin, dynein and tilB homolog protein genes); iv) aerobic metabolism (citrate synthase and carbonic anhydrase); and v) the immune-system, probably in association with haemocytes. In contrast, S individuals overexpressed a different series of genes pertaining to immune system (leucine-rich repeat protein and galectin), along with genes involved in the response to cellular stress (Heat shock protein (HSP24) and metalloendopeptidase) as well as anaerobic metabolism (C4-dicarboxylate transporter). These results might suggest that S individuals would have a greater prevalence of pathogens/diseases or a higher susceptibility to the pathogens.

Marine Environmental Research

The present study aims to test if the environmental conditions prevailing during the growing peri... more The present study aims to test if the environmental conditions prevailing during the growing period can determine the physiological profiles of specimens differentiated as fast (F) or slow (S) growers in the mussel Mytilus galloprovincialis. We reared mussel spats in the laboratory under two different conditions. In Treatment I (continuous feeding during discontinuous immersion), two mussel groups were submitted to a daily air exposure of 8 h and fed continuously during immersion-time, with either high-quality food dosed below the pseudofaeces threshold (BP group) or low organic content food dosed above the pseudofaeces threshold (AP group). In Treatment II (discontinuous feeding during continuous immersion), mussels were continuously immersed but fed only 1 day per week (RC group). Mussels were reared for 7 and 11 months (time required for size-differentiation) in Treatments I and II, respectively, and the smallest and largest individuals from each group were selected as S and F specimens. A series of feeding experiments (with different food quality, food ration and under continuous food supply) were performed to analyse the physiological performance of selected F and S mussels. In Treatment I, no significant differences were found in the metabolic rates between F and S mussels, and the faster growth rate of F-mussels resulted from their capacity to display higher clearance-ingestion rates and pre-ingestive selections. The physiological basis of growth rate differences between F and S mussels were found to be the same in mussels reared with diets below or above a pseudofaeces threshold (FBP, FAP, SBP and SAP). In contrast, the mussels from Treatment II had no significant differences in the feeding rates between FRC and SRC mussels. However, F individuals were found to have a 33% lower standard metabolic rate, indicating that fast growth under severe feeding restriction stemmed from a higher capacity of F-mussels to save energy during long periods of starvation. Despite the differences in the physiological basis explaining fast growth between the two treatments, F-mussels were found to possess significantly higher gill-surface area in both cases. It is thus concluded that endogenous factors affecting the gill-surface area play a major role in determining inter-individual growth rate differences in the mussel, Mytilus galloprovincialis.

Aquaculture International

Mytilus galloprovincialis Lmk. mussels were cultured at two culture depths in a submerged longlin... more Mytilus galloprovincialis Lmk. mussels were cultured at two culture depths in a submerged longline system, located in the SE Bay of Biscay, from June 2013 to August 2014. The Von Bertalanffy Seasonal Growth Function (VBSGF) of the original seeded batch, without newly settled seed, at each culture depth was assessed. Mussels maintained their reached size during the slow growing months and size increased thereafter. In addition, to test both culture depth scenarios for resource management, the production of commercial pieces (“small” and “large” mussels) in quantity (mussel classification) was assessed. VBSGF output proved that commercial mussels may well be harvested throughout the year in the studied site. The culture period required to produce mussels of a commercial size was 1 to 1.5 years, depending on seeding date.

Journal of the Marine Biological Association of the UK

Temporal evolution of the ingestion and absorption rates of organic matter and biochemical compon... more Temporal evolution of the ingestion and absorption rates of organic matter and biochemical components in the filter-feeding bivalve Cerastodermaedule (Mollusca: Bivalvia) fed for 12 d on the same ration (1.5 mm3 l96 h of starvation) was performed to estimate biochemical composition of metabolic faecal losses (endogenous matter lost during the process of digestion).

Hydrobiologia, 1997

Resuspension of bottom sediments by wind and tide-driven currents often occur in shallow waters, ... more Resuspension of bottom sediments by wind and tide-driven currents often occur in shallow waters, coastal embayments and estuaries. These processes may lead to dramatic variations in the concentration and organic richness of suspended particle assemblages. Since resuspended matter is mainly inorganic, decreasing organic contents are usually associated with higher seston loads. Under this environmental context the feeding behaviour of bivalves shows a wide degree of plasticity, which has been interpreted as having high adaptive value. In order to evaluate benefits derived from this behaviour, we have used functional relationships obtained in previous studies, relating feeding parameters to characteristics of suspended food, to predict the effect that different feeding responses would have exerted. In cockles, main processes determining energy acquisition are feeding rates and preingestive food selection. Thus, the procedure followed in the present work consisted of simulating rates of food absorption under alternative feeding behaviours characterised by: (a) no preferential ingestion of filtered organic matter and (b) maintenance of constant clearance rates. In the absence of selection of organic matter at the preingestive level, ingestion rate of organics (OIR) would decline with increasing seston loads to 30% of values predicted by functions fitted to experimental data; difference in absorption rate (AR) would be even greater, falling to 10%, due to the strong effect that the organic content of ingested matter exerts on absorption efficiency. On the other hand, had the clearance rate (CR) kept constant despite the increasing seston load, OIR and AR would have fallen to values respectively 30% and 49% lower than actual values. From these results it is concluded that the ability of sorting particles before ingestion and the capability of adjusting clearance rate are key elements in the feeding behaviour that enable cockles to be well adapted to cope with changes in the water column caused by resuspension events.

Journal of the Marine Biological Association of the United Kingdom, 1996

Physiological processes involved in energy acquisition by the filter-feeding bivalve Cerastoderma... more Physiological processes involved in energy acquisition by the filter-feeding bivalve Cerastoderma edule (L.) (Mollusca: Bivalvia) were quantified under naturally fluctuating feeding conditions imposed by tidal cycles in the Bay of Marennes-Oleron. Physiological measurements were performed during two neap and two spring tidal cycles in order to cover a wide range of seston concentrations (TPM = 15–95 mg I-1). The main effect exerted by tides on the food supply was the resuspension of bottom sediments of low organic content, leading to a strong ‘dilution’ of suspended organic matter.Although filtration rate was found to increase with seston concentration, ingestion rate was strictly regulated by means of pseudofaeces production. Selection efficiencies for chlorophyll a (SEchl), overall organic matter (SE0), carbon (SEC) and nitrogen (SEN) were estimated and related to dietary descriptors. The following ranking was found for the efficiency with which different substrates were selected:...

Marine Ecology Progress Series, 2001

Journal of Sea Research, 1999

... as well as seasonal changes in growth rates, have often been described (Appeldorn, 1983; Beuk... more ... as well as seasonal changes in growth rates, have often been described (Appeldorn, 1983; Beukema and Meehan, 1985; [MacDonald and Thompson, 1985a] , [MacDonald and Thompson, 1985b] and [MacDonald and Thompson, 1986] ; Iglesias and Navarro, 1990; Vincent et ...

Journal of Sea Research, 2010

... Particulate organic matter (POM: mg/l) was calculated as the difference TPM-PIM. Food organic... more ... Particulate organic matter (POM: mg/l) was calculated as the difference TPM-PIM. Food organic content (OC) was calculated as POM/TPM. 2.4. ... Lipids were extracted with a mixture of chlorophorm:methanol:water according to Bligh and Dyer (1959). ...

Journal of Experimental Marine Biology and Ecology, 1998

Journal of Experimental Marine Biology and Ecology, 2000

Journal of Experimental Marine Biology and Ecology, 1998

Journal of Comparative Physiology B, 2014

Journal of Experimental Marine Biology and Ecology, 1996

Physiological processes controlling food acquisition by the filter feeding bivalve Cerastoderma e... more Physiological processes controlling food acquisition by the filter feeding bivalve Cerastoderma edule (L.) were quantified under a broad range of seston concentrations and compositions. Experimental diets consisted of suspensions elaborated by adding variable amounts of microalgal cells of different species (or sediment particles in one case) to natural sea-water. Clearance rates exponentially decreased with seston concentration, but the rate of

Comparative Biochemistry and Physiology Part A: Molecular & Integrative Physiology, 1999

Comparative Biochemistry and Physiology Part A: Molecular & Integrative Physiology, 1998

Comparative Biochemistry and Physiology Part A: Molecular & Integrative Physiology, 2008

Les composantes physiologiques du bilan energetique ont ete determinees sur des Coques alimentees... more Les composantes physiologiques du bilan energetique ont ete determinees sur des Coques alimentees avec du seston naturel du Bassin de Marennes-Oleron (S.O. France). Ces determinations ont ete realisees a differents moments du cycle des marees de vives eaux et de mortes eaux. La variabilite nutritionelle provenant des fluctuations de l'abondance des particules en suspension est liee au flux de la maree et s'echelonne de 10 a 170 mg de matiere particulaire totale (MTP) par litre. Les variations de la fraction organique du seston peuvent etre expliquees pour 73 % par les fluctuations de la concentration en particules associees au cycle de la maree, qui comporte la remise en suspension du substrat, pauvre en contenu organique. La variabilite restante n'a pu etre expliquee par les fluctuations d'abondance du phytoplancton, car elle apparait faiblement correlee avec les concentrations des chlorophylles et/ou des phaeopigments du seston. Les equations derivees des mesures p...

Scientific Reports

We tested the hypothesis that environmental trophic conditions prominent during the growing perio... more We tested the hypothesis that environmental trophic conditions prominent during the growing period (nurture conditions) can modify the differing physiological profiles between fast (F)- and slow (S)-growing juveniles of the mussel Mytilus galloprovincialis. Approximately 200 individuals were fed a high organic content diet dosed below the pseudofaeces threshold (BP), whereas another 200 were fed a low organic content diet dosed above the pseudofaeces threshold (AP), forcing them to maintain a continuous production of pseudofaeces. After 3 months, F and S individuals in each rearing condition were selected and used in feeding experiments. We measured the physiological parameters of the energy balance of selected F and S mussels fed on 4 different diets and tested the effects of the rearing condition (BP vs AP) and growth condition (F vs S) upon the physiological variables. Irrespective of the rearing condition, F-mussels attained higher values of scope for growth with the four experi...

Aquaculture

Abstract The molecular basis underlying the mechanisms at the origin of growth variation in bival... more Abstract The molecular basis underlying the mechanisms at the origin of growth variation in bivalves is still poorly understood, although several genes have been described as upregulated in fast-growing individuals. In the present study, we reared mussel spat of the species Mytilus galloprovincialis under diets below the pseudofaeces threshold (BP) and above the pseudofaeces threshold (AP). After 3 months, F and S mussels from each condition were selected to obtain 4 experimental groups: FBP, SBP, FAP and SAP. We hypothesized that the nurturing conditions during the growing period would modify the molecular basis of their growth rate differences. To decipher the molecular mechanisms underlying the growth variation, the gill transcriptomes for the four mussel groups were analysed. Gene expression analysis revealed i) a low number (12) of genes differentially expressed in association with diet and ii) 117 genes differentially expressed by the fast- and slow-growing mussels. According to Biological Process GO term analysis transcriptomic differences between the F and S mussels were mainly based on the upregulation of: response to the stimulus, growth and cell activity. Regarding the KEGG terms, carbohydrate metabolism and the Krebs cycle were upregulated in F mussels, whereas biosynthetic processes were upregulated in S mussels. In accordance with their larger gill surface area and higher rates of feeding and growth, the F individuals overexpressed genes in their gill tissues, and these were involved in i) growth (insulin-like growth factors and myostatin); ii) maintenance of the structure and functioning of extracellular matrix (collagen, laminin, fibulin and decorin); iii) filtration and ciliary activity (mucin, fibrocystin, dynein and tilB homolog protein genes); iv) aerobic metabolism (citrate synthase and carbonic anhydrase); and v) the immune-system, probably in association with haemocytes. In contrast, S individuals overexpressed a different series of genes pertaining to immune system (leucine-rich repeat protein and galectin), along with genes involved in the response to cellular stress (Heat shock protein (HSP24) and metalloendopeptidase) as well as anaerobic metabolism (C4-dicarboxylate transporter). These results might suggest that S individuals would have a greater prevalence of pathogens/diseases or a higher susceptibility to the pathogens.

Marine Environmental Research

The present study aims to test if the environmental conditions prevailing during the growing peri... more The present study aims to test if the environmental conditions prevailing during the growing period can determine the physiological profiles of specimens differentiated as fast (F) or slow (S) growers in the mussel Mytilus galloprovincialis. We reared mussel spats in the laboratory under two different conditions. In Treatment I (continuous feeding during discontinuous immersion), two mussel groups were submitted to a daily air exposure of 8 h and fed continuously during immersion-time, with either high-quality food dosed below the pseudofaeces threshold (BP group) or low organic content food dosed above the pseudofaeces threshold (AP group). In Treatment II (discontinuous feeding during continuous immersion), mussels were continuously immersed but fed only 1 day per week (RC group). Mussels were reared for 7 and 11 months (time required for size-differentiation) in Treatments I and II, respectively, and the smallest and largest individuals from each group were selected as S and F specimens. A series of feeding experiments (with different food quality, food ration and under continuous food supply) were performed to analyse the physiological performance of selected F and S mussels. In Treatment I, no significant differences were found in the metabolic rates between F and S mussels, and the faster growth rate of F-mussels resulted from their capacity to display higher clearance-ingestion rates and pre-ingestive selections. The physiological basis of growth rate differences between F and S mussels were found to be the same in mussels reared with diets below or above a pseudofaeces threshold (FBP, FAP, SBP and SAP). In contrast, the mussels from Treatment II had no significant differences in the feeding rates between FRC and SRC mussels. However, F individuals were found to have a 33% lower standard metabolic rate, indicating that fast growth under severe feeding restriction stemmed from a higher capacity of F-mussels to save energy during long periods of starvation. Despite the differences in the physiological basis explaining fast growth between the two treatments, F-mussels were found to possess significantly higher gill-surface area in both cases. It is thus concluded that endogenous factors affecting the gill-surface area play a major role in determining inter-individual growth rate differences in the mussel, Mytilus galloprovincialis.

Aquaculture International

Mytilus galloprovincialis Lmk. mussels were cultured at two culture depths in a submerged longlin... more Mytilus galloprovincialis Lmk. mussels were cultured at two culture depths in a submerged longline system, located in the SE Bay of Biscay, from June 2013 to August 2014. The Von Bertalanffy Seasonal Growth Function (VBSGF) of the original seeded batch, without newly settled seed, at each culture depth was assessed. Mussels maintained their reached size during the slow growing months and size increased thereafter. In addition, to test both culture depth scenarios for resource management, the production of commercial pieces (“small” and “large” mussels) in quantity (mussel classification) was assessed. VBSGF output proved that commercial mussels may well be harvested throughout the year in the studied site. The culture period required to produce mussels of a commercial size was 1 to 1.5 years, depending on seeding date.

Journal of the Marine Biological Association of the UK

Temporal evolution of the ingestion and absorption rates of organic matter and biochemical compon... more Temporal evolution of the ingestion and absorption rates of organic matter and biochemical components in the filter-feeding bivalve Cerastodermaedule (Mollusca: Bivalvia) fed for 12 d on the same ration (1.5 mm3 l96 h of starvation) was performed to estimate biochemical composition of metabolic faecal losses (endogenous matter lost during the process of digestion).

Hydrobiologia, 1997

Resuspension of bottom sediments by wind and tide-driven currents often occur in shallow waters, ... more Resuspension of bottom sediments by wind and tide-driven currents often occur in shallow waters, coastal embayments and estuaries. These processes may lead to dramatic variations in the concentration and organic richness of suspended particle assemblages. Since resuspended matter is mainly inorganic, decreasing organic contents are usually associated with higher seston loads. Under this environmental context the feeding behaviour of bivalves shows a wide degree of plasticity, which has been interpreted as having high adaptive value. In order to evaluate benefits derived from this behaviour, we have used functional relationships obtained in previous studies, relating feeding parameters to characteristics of suspended food, to predict the effect that different feeding responses would have exerted. In cockles, main processes determining energy acquisition are feeding rates and preingestive food selection. Thus, the procedure followed in the present work consisted of simulating rates of food absorption under alternative feeding behaviours characterised by: (a) no preferential ingestion of filtered organic matter and (b) maintenance of constant clearance rates. In the absence of selection of organic matter at the preingestive level, ingestion rate of organics (OIR) would decline with increasing seston loads to 30% of values predicted by functions fitted to experimental data; difference in absorption rate (AR) would be even greater, falling to 10%, due to the strong effect that the organic content of ingested matter exerts on absorption efficiency. On the other hand, had the clearance rate (CR) kept constant despite the increasing seston load, OIR and AR would have fallen to values respectively 30% and 49% lower than actual values. From these results it is concluded that the ability of sorting particles before ingestion and the capability of adjusting clearance rate are key elements in the feeding behaviour that enable cockles to be well adapted to cope with changes in the water column caused by resuspension events.

Journal of the Marine Biological Association of the United Kingdom, 1996

Physiological processes involved in energy acquisition by the filter-feeding bivalve Cerastoderma... more Physiological processes involved in energy acquisition by the filter-feeding bivalve Cerastoderma edule (L.) (Mollusca: Bivalvia) were quantified under naturally fluctuating feeding conditions imposed by tidal cycles in the Bay of Marennes-Oleron. Physiological measurements were performed during two neap and two spring tidal cycles in order to cover a wide range of seston concentrations (TPM = 15–95 mg I-1). The main effect exerted by tides on the food supply was the resuspension of bottom sediments of low organic content, leading to a strong ‘dilution’ of suspended organic matter.Although filtration rate was found to increase with seston concentration, ingestion rate was strictly regulated by means of pseudofaeces production. Selection efficiencies for chlorophyll a (SEchl), overall organic matter (SE0), carbon (SEC) and nitrogen (SEN) were estimated and related to dietary descriptors. The following ranking was found for the efficiency with which different substrates were selected:...

Marine Ecology Progress Series, 2001

Journal of Sea Research, 1999

... as well as seasonal changes in growth rates, have often been described (Appeldorn, 1983; Beuk... more ... as well as seasonal changes in growth rates, have often been described (Appeldorn, 1983; Beukema and Meehan, 1985; [MacDonald and Thompson, 1985a] , [MacDonald and Thompson, 1985b] and [MacDonald and Thompson, 1986] ; Iglesias and Navarro, 1990; Vincent et ...

Journal of Sea Research, 2010

... Particulate organic matter (POM: mg/l) was calculated as the difference TPM-PIM. Food organic... more ... Particulate organic matter (POM: mg/l) was calculated as the difference TPM-PIM. Food organic content (OC) was calculated as POM/TPM. 2.4. ... Lipids were extracted with a mixture of chlorophorm:methanol:water according to Bligh and Dyer (1959). ...

Journal of Experimental Marine Biology and Ecology, 1998

Journal of Experimental Marine Biology and Ecology, 2000

Journal of Experimental Marine Biology and Ecology, 1998

Journal of Comparative Physiology B, 2014

Journal of Experimental Marine Biology and Ecology, 1996

Physiological processes controlling food acquisition by the filter feeding bivalve Cerastoderma e... more Physiological processes controlling food acquisition by the filter feeding bivalve Cerastoderma edule (L.) were quantified under a broad range of seston concentrations and compositions. Experimental diets consisted of suspensions elaborated by adding variable amounts of microalgal cells of different species (or sediment particles in one case) to natural sea-water. Clearance rates exponentially decreased with seston concentration, but the rate of

Comparative Biochemistry and Physiology Part A: Molecular & Integrative Physiology, 1999

Comparative Biochemistry and Physiology Part A: Molecular & Integrative Physiology, 1998

Comparative Biochemistry and Physiology Part A: Molecular & Integrative Physiology, 2008