Mehmet Baysallar - Academia.edu (original) (raw)
Papers by Mehmet Baysallar
Özet Bu çalýþma, bakteriyoloji laboratuvarýmýzda 2001 ile 2004 yýllarý arasýnda dýþký örneklerind... more Özet Bu çalýþma, bakteriyoloji laboratuvarýmýzda 2001 ile 2004 yýllarý arasýnda dýþký örneklerinden izole edilen 21 Salmonella ve 31 Shigella izolatý ile kan örneklerinden izole edilen 30 Salmonella izolatýnýn antibiyotik duyarlýlýklarýyla, geniþ spektrumlu beta laktamaz pozitifliklerinin saptanýp direnç paternlerinin belirlenerek tedavi protokollerine ve gelecekteki direnç geliþimlerinin izlenmesine katkýda bulunulmasý amacýyla yapýlmýþtýr. Antibiyotik duyarlýlýklarý 2005 "Clinical and Laboratory Standards Institute" kriterler-ine göre Kirby-Bauer disk difüzyon test yöntemiyle belirlen-miþtir. Shigella izolatlarýnda trimetoprim-sülfametoksazol ve tetrasikline %71, amoksisilin-klavulanik aside %9.7 oranýnda direnç gözlenmiþtir. Dýþký örneklerinden izole edilen Salmonella izolatlarýnda seftazidim, sefotaksim, trimetoprim-sülfametoksazol ve gentamisine %4.8, amok-sisilin-klavulanik aside %9.5, tetrasikline %33.3 direnç belir-lenmiþtir. Kan örneklerinden izole edilen Salmonel...
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](ÖZET Nöroparalitik bir hastalık olan botulizmi oluşturan Clostridium botulinum biyolojik silah sı... more ÖZET Nöroparalitik bir hastalık olan botulizmi oluşturan Clostridium botulinum biyolojik silah sınıflandırmasında yer aldığı için son yıllarda ayrı bir ilgi çekmektedir. Rutin laboratuar testleri ile botulizm tanısını koymak mümkün değildir. Dışkı ve gıdalardan kültür ile aynı örneklerden ve hasta serumundan toksin aranması tercih edilen işlemlerdir. Tedavide erken tanı ve destekleyici tedavi önemlidir. Botulizm ihbarı zorunlu hastalıklardandır. Çok öldürücü toksini olan bu ajanın, göz ardı edilmemesi ve laboratuar analizleri esnasında biyogüvenlik tedbirlerine uyulması gerekmektedir. SUMMARY Clostridium botulinum which causes botulism, a neuroparalytic disease, attracts attention recently because of having an important role on classification of biological warfare agents. The diagnosis of botulism is not possible with routine laboratory tests. Culture from stool and food samples or toxin detection from the same samples and sera are preferable procedures. Early diagnosis and supporti...
Revista Romana de Medicina de Laborator, 2019
Background/aim: This prospective study aimed to determine the presence of the most common carbape... more Background/aim: This prospective study aimed to determine the presence of the most common carbapenemase genes, blaOXA-48, blaKPC, blaIMP, blaVIM and blaNDM on carbapenem resistant clinical K.pneumoniae and E.coli isolates. Materials and methods: Isolates were selected according to EUCAST guideline; gradient test and disc diffusion with both meropenem and ertapenem discs. Resistance rates of these isolates to other antimicrobial agents were also examined by disc diffusion method. Carbapenem resistance gene were investigated by using Real-Time PCR. Results: A total of 3845 E. coli and 1689 K.pneumoniae isolates from clinical samples between January 2015 and April 2017 were evaluated. The 419 isolates were found as carbapenem resistant but only the first resistant isolate (n=155; 126 K.pneumoniae and 29 E.coli) of each patient were included. Carbapenem resistant isolates were most frequently isolated from intensive care units (48.8%). Colistin was the most effective antibiotic (91.0%)....
European Journal of Therapeutics
Shigellae are responsible for bacillary dysentery which causes high morbidity especially in devel... more Shigellae are responsible for bacillary dysentery which causes high morbidity especially in developing countries. in this study stool samples of 735 patients whit acute diarrhea were examined and Shigella spp. were isolated in 79(10. 7 %). S.sonnei (44.3 %) was the most frequently isolated pathogen followed by S.boydii (24.0 %) S.flexneri (22.8 %) and S.dysenteriae (8.9 %) respectively. The mean age of patients was 12.85 and 0-5 age group had the highest frequency with a rate of 41.8 %. High resistance rates were found against tetracycline, ampicilline and trimethoprim-sulfamethoxazole (SXT) which were the drug of choice for the therapy of shigellosis until recent years, while quinolones and third generation cephalosporins were found to have highest in-vitro antibacterial activity.
European Journal of Therapeutics
In this study, susceptibilities of 50 Candida strain isolated and identified from clinical specim... more In this study, susceptibilities of 50 Candida strain isolated and identified from clinical specimens as pathogen to systemic antifungal agents including amphotericin-B, flucanozole, ketocanozole and miconazole nitrate using Macro Dilution method and results were discussed in a view of literature data.
Transfusion and Apheresis Science, 2020
OBJECTIVE Parvovirus B19 causes a range of diseases and morbidity in humans and is transmissible ... more OBJECTIVE Parvovirus B19 causes a range of diseases and morbidity in humans and is transmissible by transfusion of blood, blood components and plasma derivatives. The objective of the study was to investigate the prevalence and quantity of B19 DNA among blood donors. METHOD Totally 1053 samples were collected from March to July 2016 at a blood bank for detection of Parvovirus B19 DNA and serological status of blood donors. Testing of the presence of viral DNA was performed by a quantitative real-time PCR with a 101 copies/ml detection limit. All DNA positive and randomly selected 267 samples were tested for the presence of anti-B19 IgM and IgG by ELISA. RESULTS Age distribution of donors was between 18-64; mean age was 27 and median was 23. Among the 1053 samples, 5 (0.47%) had PB19 DNA. All PB19 DNA positive donations had both B19 IgM and IgG antibodies. The DNA level for positive donations were between 0.9 × 102 to 3.1 × 104 copies/ml. IgG and IgM were present in 59.9% (160/267) and 0,74% (2/267) respectively among the healthy donors without PB19 DNA. CONCLUSION Detected DNA concentration was less than 105 copies/ml. The presence of IgM in low level PB19 DNA positive donors may indicate that there might be a risk in transmission of PB19 to particularly immunosuppressed recipients. The clinical follow-up of blood donation with low level of PB19DNA should be considered to answer the questions about blood safety.
Mikrobiyoloji Bulteni, 2015
Klinik mikrobiyoloji laboratuvarlarında metisiline dirençli Staphylococcus aureus (MRSA)'un sapta... more Klinik mikrobiyoloji laboratuvarlarında metisiline dirençli Staphylococcus aureus (MRSA)'un saptanması ve tanımlanması, uygun tedavi seçimi ve epidemiyolojik verilerin elde edilmesi için önemlidir. mecC geni, mecA genine yaklaşık %69 DNA benzerliği gösteren bir mecA homoloğudur ve PBP2a/2' proteinine yaklaşık %63 benzerlik gösteren proteini kodlamaktadır. Çeşitli çalışmalar, mecC pozitif MRSA izolatlarının, çapraz kontaminasyon ile çiftlik hayvanlarından insanlara bulaştığını desteklemektedir. Panton-Valentine lökosidini (PVL) ise, S.aureus'un potent sitotoksini olup, önemli bir virülans faktörü olarak kabul edilmektedir. Bu çalışmada, klinik örneklerden izole edilen S.aureus suşlarında mecC ve pvl gen varlığının ve prevalansının araştırılması amaçlanmıştır. Çalışmaya, Ocak 2007-Aralık 2014 tarihleri arasında hastanemizde izole edilmiş olan 1177'si metisiline duyarlı S.aureus (MSSA) ve 523'ü MRSA olmak üzere toplam 1700 S.aureus izolatı dahil edilmiştir. İzolatlar, konvansiyonel yöntemler ve BD Phoenix otomatize sistemi (BD Diagnostic Instrument Systems, ABD) ile tanımlanmıştır. Antibiyotik duyarlılık testi, oksasilin (1 μg) ve sefoksitin (30 μg) için Kirby-Bauer disk difüzyon yöntemiyle CLSI standartlarına göre yapılmıştır. mecA gen varlığı gerçek zamanlı PCR; pvl ve mecC gen varlığı ise konvansiyonel PCR yöntemi ile araştırılmıştır. Suşların izole edildiği hastaların %44.6 (759/1700)'sı poliklinik hastası ve %65.8 (1119/1700)'i erkek olup, yaş ortalaması 39.7 yıldır. Çalışmamızda değerlendirilen 1700 izolatın 523 (%30.7)'ünde mecA geni pozitif bulunmuş; hiçbir izolatta mecC geni tespit edilmemiştir. MSSA izolatlarının %1.9 (23/1177)'u ve MRSA izolatlarının %1.7 (9/523)'sinde olmak üzere, toplam 32 (%1.8) izolatta pvl geni pozitif bulunmuştur. PVL pozitif S.aureus'ların %56.2 (18/32)'sinin, deri ve yumuşak doku enfeksiyonu örneklerinden izole
Mikrobiyoloji Bulteni, 2016
Gazi Medical Journal, 2004
Annals of Laboratory Medicine, 2015
Dear Editor Resistance to broad-spectrum antimicrobials is a well-known challenge in treating Ent... more Dear Editor Resistance to broad-spectrum antimicrobials is a well-known challenge in treating Enterobacteriaceae infection worldwide. Carbapenems are an important class of antimicrobials used in treatment against these organisms, although increasing resistance to carbapenems has been reported among Enterobacteriaceae [1]. The production of OXA-48 was first described in Klebsiella pneumoniae isolates from Istanbul [2]. Although most reports describe single cases [3], important outbreaks have also been reported. K. pneumoniae and other Enterobacteriaceae strains with OXA-48 carbapenemase are now spreading from the Middle East to Europe, Asia, and North America. In addition, NDM-1 (another carbapenemase) was first identified in a K. pneumoniae isolate from a Swedish patient who had been treated in India in 2009 [4]. NDM-1 has since been reported in numerous isolates, predominantly in Escherichia coli and K. pneumonia, in many countries, including the United Kingdom, India, Pakistan, and Bangladesh [1].
The International journal of prosthodontics
This study evaluated the antibacterial activity of water-activated zinc polycarboxylate cement wi... more This study evaluated the antibacterial activity of water-activated zinc polycarboxylate cement with adjunctive 0.12% chlorhexidine gluconate on the subgingival microbiota in fixed partial dentures. Thirty-six teeth prepared as fixed partial denture abutments in 9 patients were cemented randomly using water-activated zinc polycarboxylate cement (control group) or water-activated zinc polycarboxylate cement, including 0.12% chlorhexidine gluconate (test group). A total of 108 subgingival plaque samples were analyzed at baseline, immediately before permanent cementation (5 weeks), and 8 weeks later (at 13 weeks). In the control group, the subgingival microbiota altered to closely resemble the flora of chronic gingivitis (increased proportions of gram-negative anaerobes such as Prevotella intermedia, Fusobacterium nucleatum) by 13 weeks. In contrast, the microflora at test sites comprised predominantly gram-positive facultative cocci and rods at 13 weeks. This study demonstrated that th...
Medical science monitor : international medical journal of experimental and clinical research, 2006
The isolation of Brucella species from blood may be achieved by using classic culture techniques,... more The isolation of Brucella species from blood may be achieved by using classic culture techniques, but detection of the organism is difficult due to its slow growth. The time-to-detection of Brucella can take up to 30 days using the Castaneda blood culture method. Automated blood culture systems have reduced the growth time of Brucella. In this report we would like to contribute our experience on detection time in the isolation of Brucella species from 33,039 blood culture sets using BacT/ALERT between 1995 and 2000 (13 isolates) and thereafter using both the BACTEC and BacT/ALERT systems (17 isolates). Thirty Brucella spp. (17 by both systems and 13 by BacT/ALERT only) were isolated from 33,039 blood culture sets between 1995 and 2002. Brucellae were recovered between 1.8 and 3.7 days (mean: 2.5 days) in the BacT/ALERT blood culture system and between 2.1 and 3.8 days (mean: 2.8 days) in BACTEC 9240 system. We concluded that the mean time-to-detection could be <or=3 days, which i...
Turkiye Klinikleri Journal of Medical Sciences, 2013
Mikrobiyoloji Bulteni, 2014
Anaerobe, 2007
Propolis is an agent having antimicrobial properties, however, its composition can vary depending... more Propolis is an agent having antimicrobial properties, however, its composition can vary depending on the area where it is collected. In the present study, the antimicrobial activity of five propolis samples, collected from four different regions in Turkey and from Brazil, against nine anaerobic strains was evaluated. Ethanol extracts of propolis (EEP) were prepared from propolis samples and we determined minimum inhibitory concentrations (MIC) and minimum bactericidal concentrations (MBC) of EEP on the growth of test microorganisms by using agar dilution method. All strains were susceptible and MIC values ranged from 4 to 512 mg/ml for propolis activity. Propolis from Kazan-Ankara showed most effective MIC values to the studied microorganisms. MBC values of Kazan-Ankara EEP samples were ranged from 8 to 512 mg/ml. Death was observed within 4 h of incubation for Peptostreptococcus anaerobius and micros and Lactobacillus acidophilus and Actinomyces naeslundii, while 8 h for Prevotella oralis and Prevotella melaninogenica and Porphyromonas gingivalis, 12 h for Fusobacterium nucleatum, 16 h for Veillonella parvula. It was shown that propolis samples were more effective against Gram positive anaerobic bacteria than Gram negative ones. The organic chemical compositions of EEPs were determined by high-resolution gas chromatography coupled to mass spectrometry (GC-MS). The main compounds of EEPs were flavonoids such as pinobanksin, quercetin, naringenin, galangine, chrysin and aromatic acids such as cafeic acid. Because of increased antimicrobial resistance, propolis may be kept in mind in the treatment of oral cavity diseases.
Scandinavian Journal of Infectious Diseases, 1995
There has been an increasing multiple drug resistance problem in Vibrio cholerae biotype Eltor, t... more There has been an increasing multiple drug resistance problem in Vibrio cholerae biotype Eltor, the causative agent of 7th pandemic. The aim of this study was to show in vitro and in vivo susceptibility and effectiveness of quinolones in the treatment of endemic cholera cases. Excellent results were obtained in 53 bacteriologically confirmed cholera patients treated with short-term ofloxacin and ciprofloxacin. To our knowledge, there has been no previous report on this subject in the international medical literature. Our results show that quinolones can be an alternative drug for the treatment of multiply resistant V. cholerae infections.
Microbial Drug Resistance, 2006
The purpose of this study was to describe a patient, 7-month-old child with ventriculoperitoneal ... more The purpose of this study was to describe a patient, 7-month-old child with ventriculoperitoneal shunts for hydrocephalus with ventriculitis caused by vancomycin-resistant Enterococcus faecium. Two ventriculoperitoneal shunts were inserted just after birth and on the second month. On the sixth month, both shunts were removed because of dysfunction, and external drainage was inserted. The child developed fever, and lumbar puncture revealed a high leukocyte count and protein concentration after external drainage. Cerebrospinal fluid (CSF) cultures yielded E. faecium, which was resistant to ampicillin, erythromycin, gentamicin, penicillin G, vancomycin, and teicoplanin and was susceptible to chloramphenicol, ciprofloxacin, streptomycin, levofloxacin, and rifampin, as determined by the disk diffusion method. As a result of the antimicrobial susceptibility tests, multidrug antibiotic therapy was changed from vancomycin and ceftazidime to chloramphenicol, rifampin, and meropenem. In addition, a rifampin-clindamycin-impregnated shunt (The Codman Hakim Bactiseal, Raynham, MA) was inserted. The patient became afebrile, and CSF cultures were sterile after 15 days of yielding E. faecium. Implantation of the rifampin-clindamycin-impregnated shunt and timely use of appropriate antibiotics for 10 days according to antimicrobial susceptibility testing seem to be important in the resolution of vancomycin-resistant enterococci infections, especially in countries where linezolid and quinupristin-dalfopristin are not in use yet.
Özet Bu çalýþma, bakteriyoloji laboratuvarýmýzda 2001 ile 2004 yýllarý arasýnda dýþký örneklerind... more Özet Bu çalýþma, bakteriyoloji laboratuvarýmýzda 2001 ile 2004 yýllarý arasýnda dýþký örneklerinden izole edilen 21 Salmonella ve 31 Shigella izolatý ile kan örneklerinden izole edilen 30 Salmonella izolatýnýn antibiyotik duyarlýlýklarýyla, geniþ spektrumlu beta laktamaz pozitifliklerinin saptanýp direnç paternlerinin belirlenerek tedavi protokollerine ve gelecekteki direnç geliþimlerinin izlenmesine katkýda bulunulmasý amacýyla yapýlmýþtýr. Antibiyotik duyarlýlýklarý 2005 "Clinical and Laboratory Standards Institute" kriterler-ine göre Kirby-Bauer disk difüzyon test yöntemiyle belirlen-miþtir. Shigella izolatlarýnda trimetoprim-sülfametoksazol ve tetrasikline %71, amoksisilin-klavulanik aside %9.7 oranýnda direnç gözlenmiþtir. Dýþký örneklerinden izole edilen Salmonella izolatlarýnda seftazidim, sefotaksim, trimetoprim-sülfametoksazol ve gentamisine %4.8, amok-sisilin-klavulanik aside %9.5, tetrasikline %33.3 direnç belir-lenmiþtir. Kan örneklerinden izole edilen Salmonel...
ÖZET Nöroparalitik bir hastalık olan botulizmi oluşturan Clostridium botulinum biyolojik silah sı... more ÖZET Nöroparalitik bir hastalık olan botulizmi oluşturan Clostridium botulinum biyolojik silah sınıflandırmasında yer aldığı için son yıllarda ayrı bir ilgi çekmektedir. Rutin laboratuar testleri ile botulizm tanısını koymak mümkün değildir. Dışkı ve gıdalardan kültür ile aynı örneklerden ve hasta serumundan toksin aranması tercih edilen işlemlerdir. Tedavide erken tanı ve destekleyici tedavi önemlidir. Botulizm ihbarı zorunlu hastalıklardandır. Çok öldürücü toksini olan bu ajanın, göz ardı edilmemesi ve laboratuar analizleri esnasında biyogüvenlik tedbirlerine uyulması gerekmektedir. SUMMARY Clostridium botulinum which causes botulism, a neuroparalytic disease, attracts attention recently because of having an important role on classification of biological warfare agents. The diagnosis of botulism is not possible with routine laboratory tests. Culture from stool and food samples or toxin detection from the same samples and sera are preferable procedures. Early diagnosis and supporti...
Revista Romana de Medicina de Laborator, 2019
Background/aim: This prospective study aimed to determine the presence of the most common carbape... more Background/aim: This prospective study aimed to determine the presence of the most common carbapenemase genes, blaOXA-48, blaKPC, blaIMP, blaVIM and blaNDM on carbapenem resistant clinical K.pneumoniae and E.coli isolates. Materials and methods: Isolates were selected according to EUCAST guideline; gradient test and disc diffusion with both meropenem and ertapenem discs. Resistance rates of these isolates to other antimicrobial agents were also examined by disc diffusion method. Carbapenem resistance gene were investigated by using Real-Time PCR. Results: A total of 3845 E. coli and 1689 K.pneumoniae isolates from clinical samples between January 2015 and April 2017 were evaluated. The 419 isolates were found as carbapenem resistant but only the first resistant isolate (n=155; 126 K.pneumoniae and 29 E.coli) of each patient were included. Carbapenem resistant isolates were most frequently isolated from intensive care units (48.8%). Colistin was the most effective antibiotic (91.0%)....
European Journal of Therapeutics
Shigellae are responsible for bacillary dysentery which causes high morbidity especially in devel... more Shigellae are responsible for bacillary dysentery which causes high morbidity especially in developing countries. in this study stool samples of 735 patients whit acute diarrhea were examined and Shigella spp. were isolated in 79(10. 7 %). S.sonnei (44.3 %) was the most frequently isolated pathogen followed by S.boydii (24.0 %) S.flexneri (22.8 %) and S.dysenteriae (8.9 %) respectively. The mean age of patients was 12.85 and 0-5 age group had the highest frequency with a rate of 41.8 %. High resistance rates were found against tetracycline, ampicilline and trimethoprim-sulfamethoxazole (SXT) which were the drug of choice for the therapy of shigellosis until recent years, while quinolones and third generation cephalosporins were found to have highest in-vitro antibacterial activity.
European Journal of Therapeutics
In this study, susceptibilities of 50 Candida strain isolated and identified from clinical specim... more In this study, susceptibilities of 50 Candida strain isolated and identified from clinical specimens as pathogen to systemic antifungal agents including amphotericin-B, flucanozole, ketocanozole and miconazole nitrate using Macro Dilution method and results were discussed in a view of literature data.
Transfusion and Apheresis Science, 2020
OBJECTIVE Parvovirus B19 causes a range of diseases and morbidity in humans and is transmissible ... more OBJECTIVE Parvovirus B19 causes a range of diseases and morbidity in humans and is transmissible by transfusion of blood, blood components and plasma derivatives. The objective of the study was to investigate the prevalence and quantity of B19 DNA among blood donors. METHOD Totally 1053 samples were collected from March to July 2016 at a blood bank for detection of Parvovirus B19 DNA and serological status of blood donors. Testing of the presence of viral DNA was performed by a quantitative real-time PCR with a 101 copies/ml detection limit. All DNA positive and randomly selected 267 samples were tested for the presence of anti-B19 IgM and IgG by ELISA. RESULTS Age distribution of donors was between 18-64; mean age was 27 and median was 23. Among the 1053 samples, 5 (0.47%) had PB19 DNA. All PB19 DNA positive donations had both B19 IgM and IgG antibodies. The DNA level for positive donations were between 0.9 × 102 to 3.1 × 104 copies/ml. IgG and IgM were present in 59.9% (160/267) and 0,74% (2/267) respectively among the healthy donors without PB19 DNA. CONCLUSION Detected DNA concentration was less than 105 copies/ml. The presence of IgM in low level PB19 DNA positive donors may indicate that there might be a risk in transmission of PB19 to particularly immunosuppressed recipients. The clinical follow-up of blood donation with low level of PB19DNA should be considered to answer the questions about blood safety.
Mikrobiyoloji Bulteni, 2015
Klinik mikrobiyoloji laboratuvarlarında metisiline dirençli Staphylococcus aureus (MRSA)'un sapta... more Klinik mikrobiyoloji laboratuvarlarında metisiline dirençli Staphylococcus aureus (MRSA)'un saptanması ve tanımlanması, uygun tedavi seçimi ve epidemiyolojik verilerin elde edilmesi için önemlidir. mecC geni, mecA genine yaklaşık %69 DNA benzerliği gösteren bir mecA homoloğudur ve PBP2a/2' proteinine yaklaşık %63 benzerlik gösteren proteini kodlamaktadır. Çeşitli çalışmalar, mecC pozitif MRSA izolatlarının, çapraz kontaminasyon ile çiftlik hayvanlarından insanlara bulaştığını desteklemektedir. Panton-Valentine lökosidini (PVL) ise, S.aureus'un potent sitotoksini olup, önemli bir virülans faktörü olarak kabul edilmektedir. Bu çalışmada, klinik örneklerden izole edilen S.aureus suşlarında mecC ve pvl gen varlığının ve prevalansının araştırılması amaçlanmıştır. Çalışmaya, Ocak 2007-Aralık 2014 tarihleri arasında hastanemizde izole edilmiş olan 1177'si metisiline duyarlı S.aureus (MSSA) ve 523'ü MRSA olmak üzere toplam 1700 S.aureus izolatı dahil edilmiştir. İzolatlar, konvansiyonel yöntemler ve BD Phoenix otomatize sistemi (BD Diagnostic Instrument Systems, ABD) ile tanımlanmıştır. Antibiyotik duyarlılık testi, oksasilin (1 μg) ve sefoksitin (30 μg) için Kirby-Bauer disk difüzyon yöntemiyle CLSI standartlarına göre yapılmıştır. mecA gen varlığı gerçek zamanlı PCR; pvl ve mecC gen varlığı ise konvansiyonel PCR yöntemi ile araştırılmıştır. Suşların izole edildiği hastaların %44.6 (759/1700)'sı poliklinik hastası ve %65.8 (1119/1700)'i erkek olup, yaş ortalaması 39.7 yıldır. Çalışmamızda değerlendirilen 1700 izolatın 523 (%30.7)'ünde mecA geni pozitif bulunmuş; hiçbir izolatta mecC geni tespit edilmemiştir. MSSA izolatlarının %1.9 (23/1177)'u ve MRSA izolatlarının %1.7 (9/523)'sinde olmak üzere, toplam 32 (%1.8) izolatta pvl geni pozitif bulunmuştur. PVL pozitif S.aureus'ların %56.2 (18/32)'sinin, deri ve yumuşak doku enfeksiyonu örneklerinden izole
Mikrobiyoloji Bulteni, 2016
Gazi Medical Journal, 2004
Annals of Laboratory Medicine, 2015
Dear Editor Resistance to broad-spectrum antimicrobials is a well-known challenge in treating Ent... more Dear Editor Resistance to broad-spectrum antimicrobials is a well-known challenge in treating Enterobacteriaceae infection worldwide. Carbapenems are an important class of antimicrobials used in treatment against these organisms, although increasing resistance to carbapenems has been reported among Enterobacteriaceae [1]. The production of OXA-48 was first described in Klebsiella pneumoniae isolates from Istanbul [2]. Although most reports describe single cases [3], important outbreaks have also been reported. K. pneumoniae and other Enterobacteriaceae strains with OXA-48 carbapenemase are now spreading from the Middle East to Europe, Asia, and North America. In addition, NDM-1 (another carbapenemase) was first identified in a K. pneumoniae isolate from a Swedish patient who had been treated in India in 2009 [4]. NDM-1 has since been reported in numerous isolates, predominantly in Escherichia coli and K. pneumonia, in many countries, including the United Kingdom, India, Pakistan, and Bangladesh [1].
The International journal of prosthodontics
This study evaluated the antibacterial activity of water-activated zinc polycarboxylate cement wi... more This study evaluated the antibacterial activity of water-activated zinc polycarboxylate cement with adjunctive 0.12% chlorhexidine gluconate on the subgingival microbiota in fixed partial dentures. Thirty-six teeth prepared as fixed partial denture abutments in 9 patients were cemented randomly using water-activated zinc polycarboxylate cement (control group) or water-activated zinc polycarboxylate cement, including 0.12% chlorhexidine gluconate (test group). A total of 108 subgingival plaque samples were analyzed at baseline, immediately before permanent cementation (5 weeks), and 8 weeks later (at 13 weeks). In the control group, the subgingival microbiota altered to closely resemble the flora of chronic gingivitis (increased proportions of gram-negative anaerobes such as Prevotella intermedia, Fusobacterium nucleatum) by 13 weeks. In contrast, the microflora at test sites comprised predominantly gram-positive facultative cocci and rods at 13 weeks. This study demonstrated that th...
Medical science monitor : international medical journal of experimental and clinical research, 2006
The isolation of Brucella species from blood may be achieved by using classic culture techniques,... more The isolation of Brucella species from blood may be achieved by using classic culture techniques, but detection of the organism is difficult due to its slow growth. The time-to-detection of Brucella can take up to 30 days using the Castaneda blood culture method. Automated blood culture systems have reduced the growth time of Brucella. In this report we would like to contribute our experience on detection time in the isolation of Brucella species from 33,039 blood culture sets using BacT/ALERT between 1995 and 2000 (13 isolates) and thereafter using both the BACTEC and BacT/ALERT systems (17 isolates). Thirty Brucella spp. (17 by both systems and 13 by BacT/ALERT only) were isolated from 33,039 blood culture sets between 1995 and 2002. Brucellae were recovered between 1.8 and 3.7 days (mean: 2.5 days) in the BacT/ALERT blood culture system and between 2.1 and 3.8 days (mean: 2.8 days) in BACTEC 9240 system. We concluded that the mean time-to-detection could be <or=3 days, which i...
Turkiye Klinikleri Journal of Medical Sciences, 2013
Mikrobiyoloji Bulteni, 2014
Anaerobe, 2007
Propolis is an agent having antimicrobial properties, however, its composition can vary depending... more Propolis is an agent having antimicrobial properties, however, its composition can vary depending on the area where it is collected. In the present study, the antimicrobial activity of five propolis samples, collected from four different regions in Turkey and from Brazil, against nine anaerobic strains was evaluated. Ethanol extracts of propolis (EEP) were prepared from propolis samples and we determined minimum inhibitory concentrations (MIC) and minimum bactericidal concentrations (MBC) of EEP on the growth of test microorganisms by using agar dilution method. All strains were susceptible and MIC values ranged from 4 to 512 mg/ml for propolis activity. Propolis from Kazan-Ankara showed most effective MIC values to the studied microorganisms. MBC values of Kazan-Ankara EEP samples were ranged from 8 to 512 mg/ml. Death was observed within 4 h of incubation for Peptostreptococcus anaerobius and micros and Lactobacillus acidophilus and Actinomyces naeslundii, while 8 h for Prevotella oralis and Prevotella melaninogenica and Porphyromonas gingivalis, 12 h for Fusobacterium nucleatum, 16 h for Veillonella parvula. It was shown that propolis samples were more effective against Gram positive anaerobic bacteria than Gram negative ones. The organic chemical compositions of EEPs were determined by high-resolution gas chromatography coupled to mass spectrometry (GC-MS). The main compounds of EEPs were flavonoids such as pinobanksin, quercetin, naringenin, galangine, chrysin and aromatic acids such as cafeic acid. Because of increased antimicrobial resistance, propolis may be kept in mind in the treatment of oral cavity diseases.
Scandinavian Journal of Infectious Diseases, 1995
There has been an increasing multiple drug resistance problem in Vibrio cholerae biotype Eltor, t... more There has been an increasing multiple drug resistance problem in Vibrio cholerae biotype Eltor, the causative agent of 7th pandemic. The aim of this study was to show in vitro and in vivo susceptibility and effectiveness of quinolones in the treatment of endemic cholera cases. Excellent results were obtained in 53 bacteriologically confirmed cholera patients treated with short-term ofloxacin and ciprofloxacin. To our knowledge, there has been no previous report on this subject in the international medical literature. Our results show that quinolones can be an alternative drug for the treatment of multiply resistant V. cholerae infections.
Microbial Drug Resistance, 2006
The purpose of this study was to describe a patient, 7-month-old child with ventriculoperitoneal ... more The purpose of this study was to describe a patient, 7-month-old child with ventriculoperitoneal shunts for hydrocephalus with ventriculitis caused by vancomycin-resistant Enterococcus faecium. Two ventriculoperitoneal shunts were inserted just after birth and on the second month. On the sixth month, both shunts were removed because of dysfunction, and external drainage was inserted. The child developed fever, and lumbar puncture revealed a high leukocyte count and protein concentration after external drainage. Cerebrospinal fluid (CSF) cultures yielded E. faecium, which was resistant to ampicillin, erythromycin, gentamicin, penicillin G, vancomycin, and teicoplanin and was susceptible to chloramphenicol, ciprofloxacin, streptomycin, levofloxacin, and rifampin, as determined by the disk diffusion method. As a result of the antimicrobial susceptibility tests, multidrug antibiotic therapy was changed from vancomycin and ceftazidime to chloramphenicol, rifampin, and meropenem. In addition, a rifampin-clindamycin-impregnated shunt (The Codman Hakim Bactiseal, Raynham, MA) was inserted. The patient became afebrile, and CSF cultures were sterile after 15 days of yielding E. faecium. Implantation of the rifampin-clindamycin-impregnated shunt and timely use of appropriate antibiotics for 10 days according to antimicrobial susceptibility testing seem to be important in the resolution of vancomycin-resistant enterococci infections, especially in countries where linezolid and quinupristin-dalfopristin are not in use yet.