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Papers by M. Conner
Infection and Immunity, 2005
The acute antibody and T-cell immune response to Helicobacter pylori infection in humans has not ... more The acute antibody and T-cell immune response to Helicobacter pylori infection in humans has not been studied systematically. Serum from H. pylori-naive volunteers challenged with H. pylori and cured after 4 or 12 weeks was tested by enzyme-linked immunosorbent assays for anti-H. pylori-specific immunoglobulin M (IgM) and IgA established using bacterial lysates from homologous (the infecting strain) and heterologous H. pylori. , ABC transporter binding protein, heat shock protein 70 (DnaK), and alkyl hydroperoxide reductase. Mucosal CD3, CD4, and CD8 T-cell numbers increased following infection. IgM antibody responses were detected to a range of homologous H. pylori antigens 2 to 4 weeks postchallenge. The majority of H. pylori proteins were those involved in motility and colonization and may represent targets for vaccine development.
Gastroenterology, 2001
DNA from 102 unrelated Spanish Caucasian patients with DU and 85 ethnically matched healthy contr... more DNA from 102 unrelated Spanish Caucasian patients with DU and 85 ethnically matched healthy controls, was typed for the IL-1B 5" and IL-1B -~ gene polymorphisms and the variable number of tandem repeats polymorphism in intron 2 of the IL-1RN gene by PCR based methods. The determination of H. pylori status and nonsteroidal anti-infiamatory drugs (NSAIDs) use was studied in all patients and in controls. Results: Logistic regression analysis identified H. pylori infection as an independent risk factor for duodenal ulcer (OR:10.2, 95%CI = 3.6-28.9). The simultaneous carriage of alleles IL-1RN*2, IL-IB s'*/and IL-IB *~.1 was significantly increased in H. pylori-posltive DU patients who did not take NSAIDs (n =60) compare to H. pyloriinfected healthy controls (n=63) (0R:4.08, 95%CI = 1.24-13.3). There were no differences in carriage, genotype and allele frequencies of the IL-1B and the IL-1RN gene polymorphisms between H pylori-positive and H. pylori-negative individuals, patients and controls. Moreover, no differences in genotype with respect to NSAIDs use were found between DU patients. Conclusion: Our results provide further evidence that genetic factors are important in the pathogenesis of duodenal ulcer. We suggest that the lack of the "high" IL-1,8 producer alleles, IL-1B -5" *2 and IL-1B+ ~'2, in addition to bacterial factors may play a key role in the development of duodenal ulcer disease.
Journal of Virology, 2000
We investigated the immunogenicity of recombinant double-layered rotavirus-like particle (2/6-VLP... more We investigated the immunogenicity of recombinant double-layered rotavirus-like particle (2/6-VLPs) vaccines derived from simian SA11 or human (VP6) Wa and bovine RF (VP2) rotavirus strains. The 2/6-VLPs were administered to gnotobiotic pigs intranasally (i.n.) with a mutant Escherichia coli heat-labile toxin, LT-R192G (mLT), as mucosal adjuvant. Pigs were challenged with virulent Wa (P1A[8],G1) human rotavirus at postinoculation day (PID) 21 (two-dose VLP regimen) or 28 (three-dose VLP regimen).
Infection and Immunity, 2005
The acute antibody and T-cell immune response to Helicobacter pylori infection in humans has not ... more The acute antibody and T-cell immune response to Helicobacter pylori infection in humans has not been studied systematically. Serum from H. pylori-naive volunteers challenged with H. pylori and cured after 4 or 12 weeks was tested by enzyme-linked immunosorbent assays for anti-H. pylori-specific immunoglobulin M (IgM) and IgA established using bacterial lysates from homologous (the infecting strain) and heterologous H. pylori. , ABC transporter binding protein, heat shock protein 70 (DnaK), and alkyl hydroperoxide reductase. Mucosal CD3, CD4, and CD8 T-cell numbers increased following infection. IgM antibody responses were detected to a range of homologous H. pylori antigens 2 to 4 weeks postchallenge. The majority of H. pylori proteins were those involved in motility and colonization and may represent targets for vaccine development.
Gastroenterology, 2001
DNA from 102 unrelated Spanish Caucasian patients with DU and 85 ethnically matched healthy contr... more DNA from 102 unrelated Spanish Caucasian patients with DU and 85 ethnically matched healthy controls, was typed for the IL-1B 5" and IL-1B -~ gene polymorphisms and the variable number of tandem repeats polymorphism in intron 2 of the IL-1RN gene by PCR based methods. The determination of H. pylori status and nonsteroidal anti-infiamatory drugs (NSAIDs) use was studied in all patients and in controls. Results: Logistic regression analysis identified H. pylori infection as an independent risk factor for duodenal ulcer (OR:10.2, 95%CI = 3.6-28.9). The simultaneous carriage of alleles IL-1RN*2, IL-IB s'*/and IL-IB *~.1 was significantly increased in H. pylori-posltive DU patients who did not take NSAIDs (n =60) compare to H. pyloriinfected healthy controls (n=63) (0R:4.08, 95%CI = 1.24-13.3). There were no differences in carriage, genotype and allele frequencies of the IL-1B and the IL-1RN gene polymorphisms between H pylori-positive and H. pylori-negative individuals, patients and controls. Moreover, no differences in genotype with respect to NSAIDs use were found between DU patients. Conclusion: Our results provide further evidence that genetic factors are important in the pathogenesis of duodenal ulcer. We suggest that the lack of the "high" IL-1,8 producer alleles, IL-1B -5" *2 and IL-1B+ ~'2, in addition to bacterial factors may play a key role in the development of duodenal ulcer disease.
Journal of Virology, 2000
We investigated the immunogenicity of recombinant double-layered rotavirus-like particle (2/6-VLP... more We investigated the immunogenicity of recombinant double-layered rotavirus-like particle (2/6-VLPs) vaccines derived from simian SA11 or human (VP6) Wa and bovine RF (VP2) rotavirus strains. The 2/6-VLPs were administered to gnotobiotic pigs intranasally (i.n.) with a mutant Escherichia coli heat-labile toxin, LT-R192G (mLT), as mucosal adjuvant. Pigs were challenged with virulent Wa (P1A[8],G1) human rotavirus at postinoculation day (PID) 21 (two-dose VLP regimen) or 28 (three-dose VLP regimen).