MD ASIF ALI - Academia.edu (original) (raw)

Papers by MD ASIF ALI

Energies

The scope of this work was to develop a technique based on the regression method and apply it on ... more The scope of this work was to develop a technique based on the regression method and apply it on a real cooled geometry for measuring its internal heat transfer distribution. The proposed methodology is based upon an already available literature approach. For implementation of the methodology, the geometry is initially heated to a known steady temperature, followed by thermal transient, induced by injection of ambient air to its internal cooling system. During the thermal transient, external surface temperature of the geometry is recorded with the help of infrared camera. Then, a numerical procedure based upon a series of transient finite element analyses of the geometry is applied by using the obtained experimental data. The total test duration is divided into time steps, during which the heat flux on the internal surface is iteratively updated to target the measured external surface temperature. The final procured heat flux and internal surface temperature data of each time step i...

E3S Web of Conferences

To ensure a passable life span of gas turbine hot gas path components the measurement of metal su... more To ensure a passable life span of gas turbine hot gas path components the measurement of metal surface temperature is paramount. Experimental analyses on internally cooled devices are often performed on simplified or scaled up geometries, which reduces the applicability of the results to the actual real hardware. A more reliable estimation of cooling performance could be obtained if the real engine component is directly studied. To achieve this goal, an experimental campaign is performed to investigate the internal heat transfer distribution of an industrial blade, cooled by means of an internal U-shaped channel. During the experiment the blade is heated to a known temperature, then a coolant is introduced through the internal channel to induce a thermal transient, during which the external surface temperature is measured with the help of an infrared camera. Then a transient thermal finite element simulation is performed with the same boundary and inlet conditions of the experiment....

Plants

Phosphorus (P) is an essential macroelement supporting maize productivity and low-P stress is a l... more Phosphorus (P) is an essential macroelement supporting maize productivity and low-P stress is a limiting factor of maize growth and yield. Improving maize plant tolerance to low P through molecular breeding is an effective alternative to increase crop productivity. In this study, a total of 111 diverse maize inbred lines were used to identify the favorable alleles and nucleotide diversity of candidate ZmNAC9, which plays an important role in response to low P and regulation in root architecture. A significant difference was found under low- and sufficient-P conditions for each of the 22 seedling traits, and a total of 41 polymorphic sites including 32 single nucleotide polymorphisms (SNPs) and 9 insertion and deletions (InDels) were detected in ZmNAC9 among 111 inbred lines. Among the 41 polymorphic studied sites, a total of 39 polymorphic sites were associated with 20 traits except for the dry weight of shoots and forks, of which six sites were highly significantly associated with ...

South African Journal of Industrial Engineering

Organisations cannot put their efforts equally into all the best practices that impact operationa... more Organisations cannot put their efforts equally into all the best practices that impact operational performance, due to their limited resources, their ineffective usage, government policies, and inadequate strategic planning. In this contribution, we aim to identify and prioritise the practices with the most impact on operational performance. Twelve practices that steer operational performance were identified through a literature survey. Second, the identified practices were prioritised and evaluated, based on improving operational performance by using a hybrid approach. The analytical hierarchy process (AHP) and the technique for order performance by similarity to ideal solution (TOPSIS) method were used such that AHP was applied to determine the relative weights of alternatives, and then the final ranking was obtained by conducting a TOPSIS analysis. Empirical data were collected from eight experts and 417 users at operational-level. The results suggest that lean management, total quality management, six sigma, and supply chain management practices rank 1st, 2nd, 3rd, and 4th respectively, signifying the practices with the most influence and making the greatest contribution. This strategic understanding will help researchers and practitioners to frame competitive strategies in order to pay more attention to those practices that have a greater influence on operational performance.

Mathematical Problems in Engineering

For achieving world-class performance and competitiveness in today’s global highly complex enviro... more For achieving world-class performance and competitiveness in today’s global highly complex environment and competition, there is an immense pressure on manufacturing organizations having limited resources to pursue operational excellence in terms of productivity and quality, reduce cost, and provide high-quality products in shorter lead times. Identification of representative practices affecting operational performance (OP) and understanding of mutual dependence among those practices will improve the performance of manufacturing organizations with available resources. A sample of 10 experts of academicians and senior company managers from different functional areas of large-scale textile, sugar, and cement industries were surveyed to identify critical practices influencing OP and then to develop the relationships among them. It also identifies those practices that support other practices (driving practices) and also those that are most influenced by other practices (dependent practi...

SECOND INTERNATIONAL CONFERENCE ON MATERIAL SCIENCE, SMART STRUCTURES AND APPLICATIONS: ICMSS-2019

The aim of this work is to develop a suitable technique to measure internal heat transfer coeffic... more The aim of this work is to develop a suitable technique to measure internal heat transfer coefficient distributions of a high conductivity device (e.g. a gas turbine blade). The technique implements the acquisition of external full surface temperature data of the geometry during the thermal transient evoked by internal cooling, in concurrence with geometry material property details, part wall thickness and internal fluid temperature. With the help of an infrared camera the external temperature history of the geometry is recorded during the whole thermal transient. Infrared data is combined with cooling flow network modeling to provide the boundary conditions for a finite element model representing the geometry. An inverse transient conduction model of the geometry is executed with updated internal heat transfer coefficient distribution until the convergence is reached with the experimental measured external wall temperature at a given time. The first iteration of internal heat transfer coefficient distribution is calculated by using lumped thermal capacitance model. Then a root finding algorithm is used for further iterations until convergence is achieved. The technique is validated on a canonical test case composed by a straight circular duct. The experimental setup consists of an oven which contains ceramic heaters, an air intake system used for flowing air inside the duct and a data acquisition system consisting of Coriolis flow meter, thermocouples and an infrared camera. First the duct is heated to uniform temperature in the oven, then an air is introduced into the duct in order to induce a thermal transient. Reynolds number of 25000, 50000 and 75000 are used for different tests. The outcomes of different tests are compared with available literature data, obtaining a good agreement and thus validating the employed experimental approach.

Proceedings of the Institution of Mechanical Engineers, Part G: Journal of Aerospace Engineering

Journal of Mathematics of Kyoto University

... Following is a short proof of the result given by Laradji and Thaheem in [5]. Theorem 0.4. Le... more ... Following is a short proof of the result given by Laradji and Thaheem in [5]. Theorem 0.4. Let R be a semiprime ring and f be a centralizing au-tomorphism of R. Then D(f) ⊆ Z(R). Proof. ... i i i i 246 Asif Ali and Muhammad Yasen By Theorem 0.1, a(f(x) − x)[y, z]=[y, z](f(x) − x)a = 0. ...

Bulletin of the Korean Mathematical Society, 2006

Let R be a semiprime ring and f be an endomorphism on R. If f is a strong commutativity preservin... more Let R be a semiprime ring and f be an endomorphism on R. If f is a strong commutativity preserving (simply, scp) map on a non-zero ideal U of R, then f is commuting on U .

Bioinformation, Dec 6, 2013

Background: Arabitol dehydrogenase (ArDH) is involved in the production of different sugar alcoho... more Background: Arabitol dehydrogenase (ArDH) is involved in the production of different sugar alcohols like arabitol, sorbitol, mannitol, erythritol and xylitol by using five carbon sugars as substrate. Arabinose, d-ribose, d-ribulose, xylose and d-xylulose are known substrate of this enzyme. ArDH is mainly produced by osmophilic fungi for the conversion of ribulose to arabitol under stress conditions. Recently this enzyme has been used by various industries for the production of pharmaceutically important sugar alcohols form cheap source than glucose. But the information at structure level as well as its binding energy analysis with different substrates was missing. Results: The present study was focused on sequence analysis, insilico characterization and substrate binding analysis of ArDH from a fungus specie candida albican. Sequence analysis and physicochemical properties showed that this protein is highly stable, negatively charged and having more hydrophilic regions, these properties made this enzyme to bind with number of five carbon sugars as substrate. The predicted 3D model will helpful for further structure based studies. Docking analysis provided free energies of binding of each substrate from a best pose as arabinose-9.8224Kcal/mol, dribose-11.3701Kcal/mol, d-ribulose-8.9230Kcal/mol, xylose-9.7007Kcal/mol and d-xylulose 9.7802Kcal/mol. Conclusion: Our study provided insight information of structure and interactions of ArDH with its substrate. The results obtained from this study clearly indicate that d-ribose is the best substrate for ArDH for the production of sugar alcohols. This information will be helpful for better usage of this enzyme for hyper-production of sugar alcohols by different industries.

Biochemistry and Molecular Biology International, Oct 1, 1997

Native calf thymus DNA was modified with furocoumarins and reactive oxygen species (ROS). The mod... more Native calf thymus DNA was modified with furocoumarins and reactive oxygen species (ROS). The modifications were probed by UV & Fluorescence spectroscopy, thermal denaturation and hydroxyapatite chromatography. In CD spectroscopy changes in the ellipticity of the DNA molecule were observed as a result of modification. The binding specificity of naturally occurring anti-DNA antibodies with modified DNA molecules was assessed by ELISA and quantitative precipitin titration. The affinity of anti-DNA antibodies for modified conformers was found to be quite high.

Mathematical and Computational Applications, 2005

Exact solutions for the unsteady flow equations of an incompressible MHD aligned second grade flu... more Exact solutions for the unsteady flow equations of an incompressible MHD aligned second grade fluid are obtained. Translational symmetries are used successively to reduce the governing partial differential equations into ordinary differential equations.

Biochimica Et Biophysica Acta General Subjects, Apr 9, 1991

The DNA-psoralen crosslink induced precipitating antibodies in rabbits with a titer of 1:102,400 ... more The DNA-psoralen crosslink induced precipitating antibodies in rabbits with a titer of 1:102,400 by direct binding ELISA. The antiserum showed considerable binding with Z-DNA and calf thymus DNA brominated under high salt concentration which has been shown to attain Z-/analogous conformation. Inhibition experiments substantiated the results of direct binding assay. However, the affinity purified IgG showed high degree of specificity for the immunogen and did not recognize nDNA, Z-DNA and brominated DNA as inhibitor. Poly(dG.dC).poly(dG.dC)-psoralen photoadduct was found to be inhibitory. These results indicate that the antibodies are probably recognizing the unique conformation at the site of psoralen crosslinking. The DNA-psoralen crosslink showed significant binding with SLE sera known to have high levels of anti-native DNA antibodies. Affinity purified SLE-IgG in a competition assay pointed out the autoantibody recognition of altered conformation of DNA-psoralen crosslink.

... 1 , Brian Harding 1 , M Andrew Nesbit 1 , Rebecca Fairclough 1 , Elena Grigorieva 2 , Asif Al... more ... 1 , Brian Harding 1 , M Andrew Nesbit 1 , Rebecca Fairclough 1 , Elena Grigorieva 2 , Asif Ali 1 , Tertius Hough 5 , William ... 3 Royal Liverpool University Hospital, Liverpool, United Kingdom; 4 Erasmus University, Rotterdam, Rotterdam, Netherlands; 5 Mary Lyon Centre, Medical ...

Jahrbuch Fur Morphologie Und Mikroskopische Anatomie 2 Abteilung Zeitschrift Fur Mikroskopisch Anatomische Forschung, Feb 1, 1984

Rabbits immunized with polylysine-polyglutamate complex induced high titer antibodies. The immune... more Rabbits immunized with polylysine-polyglutamate complex induced high titer antibodies. The immune IgG exhibited high degree of specificity towards the immunogen. In a competition assay, nDNA, heat denatured DNA and total buffalo thymus RNA were inhibitory. An appreciable binding with polyglutamate, poly(rG).poly(dC) poly(dG).poly(dC), cardiolipin, poly(dA-dU). Poly(dA-dU) and Z- or Z-like conformations demonstrates the polyspecificity of induced antibodies. The immunological specificity of induced antibodies was comparable to autoantibodies derived from SLE. The results might be of some help in understanding the etiology of autoimmune process.

International Union of Biochemistry and Molecular Biology Life, Mar 5, 2015

Hyperglycemia is the defining feature of diabetes mellitus. The persistently high levels of reduc... more Hyperglycemia is the defining feature of diabetes mellitus. The persistently high levels of reducing sugars like glucose and fructose cause glycation of various macromolecules in the body. Human serum albumin (HSA), the most abundant serum protein with a myriad of functions, is prone to glycation and consequent alteration in its structural and biological properties. This study aimed to assess the role of fructose-modified human serum albumin as a marker of diabetic pathophysiology. We carried out modification of HSA with fructose and the changes induced were studied by various physicochemical studies. Fructose modified-HSA showed hyperchromicity in UV spectrum and increased AGE-specific fluorescence as well as quenching of tryptophan fluorescence. In SDS-PAGE protein aggregation was seen. Amadori products were detected by NBT. The fructose modified HSA had higher content of carbonyls along with perturbations in secondary structure as revealed by CD and FT-IR. A greater hydrodynamic radius of fructose-modified HSA was evident by DLS measurement. The fructose-modified HSA induced high titre antibodies in experimental animals exhibiting high specificity towards the immunogen. © 2015 IUBMB Life, 2015 67(5):338-347, 2015.

ABSTRACT INTRODUCTION Pancreatic ductal adenocarcinoma (PDAC) is common and aggressive with a 5-y... more ABSTRACT INTRODUCTION Pancreatic ductal adenocarcinoma (PDAC) is common and aggressive with a 5-year survival of 2-3%. Initial assessment of patients involves imaging and diagnostic cytology at endoscopy. Pre-treatment diagnosis on cytology samples can be difficult to establish, and may be improved with diagnostic biomarkers. KOC (k homology domain protein) is highly expressed in PDAC but less in normal ducts. Therefore, KOC could help to increase the diagnostic accuracy of pancreatic ductal adenocarcinoma (PDAC) versus benign or inflammatory tissue. Our aim was to validate the expression of KOC in a local surgical cohort. MATERIAL AND METHODS Optimised antibodies for KOC were applied to 3 Tissue Microarray (TMA) slides containing a total of 256 cores (149 PDAC, 107 Normal Ducts) from 89 patients. Afterwards, we analysed the immunohistochemical expression of KOC with the intension of differentiating PDAC from normal pancreatic ducts. We selected cores for comparison purposes as negative, weak, moderate and strong for staining. The expression was visualised as a cytoplasmic stain of varying intensity and proportion in distiller 2.1. Results were quantitatively measured as a “weighted histoscore”, taking into account both the intensity of staining and the proportion of cells being positive [Histoscore= (negative=0×%cells + weak=1×%cells + moderate=2×%cells + strong=3×%cells)]. RESULTS Immunohistochemical analysis of KOC expression showed heterogeneity of staining in PDAC but not in normal ductal tissues. Overall average percentage of KOC expressing cells across all cores in 3 TMAs for PDAC samples was 77.9% (range, 0%-100%), however in normal pancreatic ducts it was 0.32% (range, 0%-10%) [p <.0001, Independent sample t test]. Similarly, the average histoscore for PDAC samples was 153 (range, 0-300), while for normal ducts it was 0.52 (range, 0-18) [p <.0001, Independent sample t test]. Finally, with a cut-off of 12.5% positivity of cells and at least weak staining, a sensitivity of 83.9% and specificity of 100% was achieved. CONCLUSION The results of this study with 100% specificity and almost 84% sensitivity demonstrate that KOC expression is essentially restricted to PDAC in tissue samples. Thus it has the potential to increase the diagnostic accuracy of cytological samples of PDAC, but further studies on cytologic samples of PDAC and normal ducts are required. J Pathol Inform. 2011; 2: 43c. > Sub-article 2

African Journal of Urology, Apr 18, 2006

Energies

The scope of this work was to develop a technique based on the regression method and apply it on ... more The scope of this work was to develop a technique based on the regression method and apply it on a real cooled geometry for measuring its internal heat transfer distribution. The proposed methodology is based upon an already available literature approach. For implementation of the methodology, the geometry is initially heated to a known steady temperature, followed by thermal transient, induced by injection of ambient air to its internal cooling system. During the thermal transient, external surface temperature of the geometry is recorded with the help of infrared camera. Then, a numerical procedure based upon a series of transient finite element analyses of the geometry is applied by using the obtained experimental data. The total test duration is divided into time steps, during which the heat flux on the internal surface is iteratively updated to target the measured external surface temperature. The final procured heat flux and internal surface temperature data of each time step i...

E3S Web of Conferences

To ensure a passable life span of gas turbine hot gas path components the measurement of metal su... more To ensure a passable life span of gas turbine hot gas path components the measurement of metal surface temperature is paramount. Experimental analyses on internally cooled devices are often performed on simplified or scaled up geometries, which reduces the applicability of the results to the actual real hardware. A more reliable estimation of cooling performance could be obtained if the real engine component is directly studied. To achieve this goal, an experimental campaign is performed to investigate the internal heat transfer distribution of an industrial blade, cooled by means of an internal U-shaped channel. During the experiment the blade is heated to a known temperature, then a coolant is introduced through the internal channel to induce a thermal transient, during which the external surface temperature is measured with the help of an infrared camera. Then a transient thermal finite element simulation is performed with the same boundary and inlet conditions of the experiment....

Plants

Phosphorus (P) is an essential macroelement supporting maize productivity and low-P stress is a l... more Phosphorus (P) is an essential macroelement supporting maize productivity and low-P stress is a limiting factor of maize growth and yield. Improving maize plant tolerance to low P through molecular breeding is an effective alternative to increase crop productivity. In this study, a total of 111 diverse maize inbred lines were used to identify the favorable alleles and nucleotide diversity of candidate ZmNAC9, which plays an important role in response to low P and regulation in root architecture. A significant difference was found under low- and sufficient-P conditions for each of the 22 seedling traits, and a total of 41 polymorphic sites including 32 single nucleotide polymorphisms (SNPs) and 9 insertion and deletions (InDels) were detected in ZmNAC9 among 111 inbred lines. Among the 41 polymorphic studied sites, a total of 39 polymorphic sites were associated with 20 traits except for the dry weight of shoots and forks, of which six sites were highly significantly associated with ...

South African Journal of Industrial Engineering

Organisations cannot put their efforts equally into all the best practices that impact operationa... more Organisations cannot put their efforts equally into all the best practices that impact operational performance, due to their limited resources, their ineffective usage, government policies, and inadequate strategic planning. In this contribution, we aim to identify and prioritise the practices with the most impact on operational performance. Twelve practices that steer operational performance were identified through a literature survey. Second, the identified practices were prioritised and evaluated, based on improving operational performance by using a hybrid approach. The analytical hierarchy process (AHP) and the technique for order performance by similarity to ideal solution (TOPSIS) method were used such that AHP was applied to determine the relative weights of alternatives, and then the final ranking was obtained by conducting a TOPSIS analysis. Empirical data were collected from eight experts and 417 users at operational-level. The results suggest that lean management, total quality management, six sigma, and supply chain management practices rank 1st, 2nd, 3rd, and 4th respectively, signifying the practices with the most influence and making the greatest contribution. This strategic understanding will help researchers and practitioners to frame competitive strategies in order to pay more attention to those practices that have a greater influence on operational performance.

Mathematical Problems in Engineering

For achieving world-class performance and competitiveness in today’s global highly complex enviro... more For achieving world-class performance and competitiveness in today’s global highly complex environment and competition, there is an immense pressure on manufacturing organizations having limited resources to pursue operational excellence in terms of productivity and quality, reduce cost, and provide high-quality products in shorter lead times. Identification of representative practices affecting operational performance (OP) and understanding of mutual dependence among those practices will improve the performance of manufacturing organizations with available resources. A sample of 10 experts of academicians and senior company managers from different functional areas of large-scale textile, sugar, and cement industries were surveyed to identify critical practices influencing OP and then to develop the relationships among them. It also identifies those practices that support other practices (driving practices) and also those that are most influenced by other practices (dependent practi...

SECOND INTERNATIONAL CONFERENCE ON MATERIAL SCIENCE, SMART STRUCTURES AND APPLICATIONS: ICMSS-2019

The aim of this work is to develop a suitable technique to measure internal heat transfer coeffic... more The aim of this work is to develop a suitable technique to measure internal heat transfer coefficient distributions of a high conductivity device (e.g. a gas turbine blade). The technique implements the acquisition of external full surface temperature data of the geometry during the thermal transient evoked by internal cooling, in concurrence with geometry material property details, part wall thickness and internal fluid temperature. With the help of an infrared camera the external temperature history of the geometry is recorded during the whole thermal transient. Infrared data is combined with cooling flow network modeling to provide the boundary conditions for a finite element model representing the geometry. An inverse transient conduction model of the geometry is executed with updated internal heat transfer coefficient distribution until the convergence is reached with the experimental measured external wall temperature at a given time. The first iteration of internal heat transfer coefficient distribution is calculated by using lumped thermal capacitance model. Then a root finding algorithm is used for further iterations until convergence is achieved. The technique is validated on a canonical test case composed by a straight circular duct. The experimental setup consists of an oven which contains ceramic heaters, an air intake system used for flowing air inside the duct and a data acquisition system consisting of Coriolis flow meter, thermocouples and an infrared camera. First the duct is heated to uniform temperature in the oven, then an air is introduced into the duct in order to induce a thermal transient. Reynolds number of 25000, 50000 and 75000 are used for different tests. The outcomes of different tests are compared with available literature data, obtaining a good agreement and thus validating the employed experimental approach.

Proceedings of the Institution of Mechanical Engineers, Part G: Journal of Aerospace Engineering

Journal of Mathematics of Kyoto University

... Following is a short proof of the result given by Laradji and Thaheem in [5]. Theorem 0.4. Le... more ... Following is a short proof of the result given by Laradji and Thaheem in [5]. Theorem 0.4. Let R be a semiprime ring and f be a centralizing au-tomorphism of R. Then D(f) ⊆ Z(R). Proof. ... i i i i 246 Asif Ali and Muhammad Yasen By Theorem 0.1, a(f(x) − x)[y, z]=[y, z](f(x) − x)a = 0. ...

Bulletin of the Korean Mathematical Society, 2006

Let R be a semiprime ring and f be an endomorphism on R. If f is a strong commutativity preservin... more Let R be a semiprime ring and f be an endomorphism on R. If f is a strong commutativity preserving (simply, scp) map on a non-zero ideal U of R, then f is commuting on U .

Bioinformation, Dec 6, 2013

Background: Arabitol dehydrogenase (ArDH) is involved in the production of different sugar alcoho... more Background: Arabitol dehydrogenase (ArDH) is involved in the production of different sugar alcohols like arabitol, sorbitol, mannitol, erythritol and xylitol by using five carbon sugars as substrate. Arabinose, d-ribose, d-ribulose, xylose and d-xylulose are known substrate of this enzyme. ArDH is mainly produced by osmophilic fungi for the conversion of ribulose to arabitol under stress conditions. Recently this enzyme has been used by various industries for the production of pharmaceutically important sugar alcohols form cheap source than glucose. But the information at structure level as well as its binding energy analysis with different substrates was missing. Results: The present study was focused on sequence analysis, insilico characterization and substrate binding analysis of ArDH from a fungus specie candida albican. Sequence analysis and physicochemical properties showed that this protein is highly stable, negatively charged and having more hydrophilic regions, these properties made this enzyme to bind with number of five carbon sugars as substrate. The predicted 3D model will helpful for further structure based studies. Docking analysis provided free energies of binding of each substrate from a best pose as arabinose-9.8224Kcal/mol, dribose-11.3701Kcal/mol, d-ribulose-8.9230Kcal/mol, xylose-9.7007Kcal/mol and d-xylulose 9.7802Kcal/mol. Conclusion: Our study provided insight information of structure and interactions of ArDH with its substrate. The results obtained from this study clearly indicate that d-ribose is the best substrate for ArDH for the production of sugar alcohols. This information will be helpful for better usage of this enzyme for hyper-production of sugar alcohols by different industries.

Biochemistry and Molecular Biology International, Oct 1, 1997

Native calf thymus DNA was modified with furocoumarins and reactive oxygen species (ROS). The mod... more Native calf thymus DNA was modified with furocoumarins and reactive oxygen species (ROS). The modifications were probed by UV & Fluorescence spectroscopy, thermal denaturation and hydroxyapatite chromatography. In CD spectroscopy changes in the ellipticity of the DNA molecule were observed as a result of modification. The binding specificity of naturally occurring anti-DNA antibodies with modified DNA molecules was assessed by ELISA and quantitative precipitin titration. The affinity of anti-DNA antibodies for modified conformers was found to be quite high.

Mathematical and Computational Applications, 2005

Exact solutions for the unsteady flow equations of an incompressible MHD aligned second grade flu... more Exact solutions for the unsteady flow equations of an incompressible MHD aligned second grade fluid are obtained. Translational symmetries are used successively to reduce the governing partial differential equations into ordinary differential equations.

Biochimica Et Biophysica Acta General Subjects, Apr 9, 1991

The DNA-psoralen crosslink induced precipitating antibodies in rabbits with a titer of 1:102,400 ... more The DNA-psoralen crosslink induced precipitating antibodies in rabbits with a titer of 1:102,400 by direct binding ELISA. The antiserum showed considerable binding with Z-DNA and calf thymus DNA brominated under high salt concentration which has been shown to attain Z-/analogous conformation. Inhibition experiments substantiated the results of direct binding assay. However, the affinity purified IgG showed high degree of specificity for the immunogen and did not recognize nDNA, Z-DNA and brominated DNA as inhibitor. Poly(dG.dC).poly(dG.dC)-psoralen photoadduct was found to be inhibitory. These results indicate that the antibodies are probably recognizing the unique conformation at the site of psoralen crosslinking. The DNA-psoralen crosslink showed significant binding with SLE sera known to have high levels of anti-native DNA antibodies. Affinity purified SLE-IgG in a competition assay pointed out the autoantibody recognition of altered conformation of DNA-psoralen crosslink.

... 1 , Brian Harding 1 , M Andrew Nesbit 1 , Rebecca Fairclough 1 , Elena Grigorieva 2 , Asif Al... more ... 1 , Brian Harding 1 , M Andrew Nesbit 1 , Rebecca Fairclough 1 , Elena Grigorieva 2 , Asif Ali 1 , Tertius Hough 5 , William ... 3 Royal Liverpool University Hospital, Liverpool, United Kingdom; 4 Erasmus University, Rotterdam, Rotterdam, Netherlands; 5 Mary Lyon Centre, Medical ...

Jahrbuch Fur Morphologie Und Mikroskopische Anatomie 2 Abteilung Zeitschrift Fur Mikroskopisch Anatomische Forschung, Feb 1, 1984

Rabbits immunized with polylysine-polyglutamate complex induced high titer antibodies. The immune... more Rabbits immunized with polylysine-polyglutamate complex induced high titer antibodies. The immune IgG exhibited high degree of specificity towards the immunogen. In a competition assay, nDNA, heat denatured DNA and total buffalo thymus RNA were inhibitory. An appreciable binding with polyglutamate, poly(rG).poly(dC) poly(dG).poly(dC), cardiolipin, poly(dA-dU). Poly(dA-dU) and Z- or Z-like conformations demonstrates the polyspecificity of induced antibodies. The immunological specificity of induced antibodies was comparable to autoantibodies derived from SLE. The results might be of some help in understanding the etiology of autoimmune process.

International Union of Biochemistry and Molecular Biology Life, Mar 5, 2015

Hyperglycemia is the defining feature of diabetes mellitus. The persistently high levels of reduc... more Hyperglycemia is the defining feature of diabetes mellitus. The persistently high levels of reducing sugars like glucose and fructose cause glycation of various macromolecules in the body. Human serum albumin (HSA), the most abundant serum protein with a myriad of functions, is prone to glycation and consequent alteration in its structural and biological properties. This study aimed to assess the role of fructose-modified human serum albumin as a marker of diabetic pathophysiology. We carried out modification of HSA with fructose and the changes induced were studied by various physicochemical studies. Fructose modified-HSA showed hyperchromicity in UV spectrum and increased AGE-specific fluorescence as well as quenching of tryptophan fluorescence. In SDS-PAGE protein aggregation was seen. Amadori products were detected by NBT. The fructose modified HSA had higher content of carbonyls along with perturbations in secondary structure as revealed by CD and FT-IR. A greater hydrodynamic radius of fructose-modified HSA was evident by DLS measurement. The fructose-modified HSA induced high titre antibodies in experimental animals exhibiting high specificity towards the immunogen. © 2015 IUBMB Life, 2015 67(5):338-347, 2015.

ABSTRACT INTRODUCTION Pancreatic ductal adenocarcinoma (PDAC) is common and aggressive with a 5-y... more ABSTRACT INTRODUCTION Pancreatic ductal adenocarcinoma (PDAC) is common and aggressive with a 5-year survival of 2-3%. Initial assessment of patients involves imaging and diagnostic cytology at endoscopy. Pre-treatment diagnosis on cytology samples can be difficult to establish, and may be improved with diagnostic biomarkers. KOC (k homology domain protein) is highly expressed in PDAC but less in normal ducts. Therefore, KOC could help to increase the diagnostic accuracy of pancreatic ductal adenocarcinoma (PDAC) versus benign or inflammatory tissue. Our aim was to validate the expression of KOC in a local surgical cohort. MATERIAL AND METHODS Optimised antibodies for KOC were applied to 3 Tissue Microarray (TMA) slides containing a total of 256 cores (149 PDAC, 107 Normal Ducts) from 89 patients. Afterwards, we analysed the immunohistochemical expression of KOC with the intension of differentiating PDAC from normal pancreatic ducts. We selected cores for comparison purposes as negative, weak, moderate and strong for staining. The expression was visualised as a cytoplasmic stain of varying intensity and proportion in distiller 2.1. Results were quantitatively measured as a “weighted histoscore”, taking into account both the intensity of staining and the proportion of cells being positive [Histoscore= (negative=0×%cells + weak=1×%cells + moderate=2×%cells + strong=3×%cells)]. RESULTS Immunohistochemical analysis of KOC expression showed heterogeneity of staining in PDAC but not in normal ductal tissues. Overall average percentage of KOC expressing cells across all cores in 3 TMAs for PDAC samples was 77.9% (range, 0%-100%), however in normal pancreatic ducts it was 0.32% (range, 0%-10%) [p <.0001, Independent sample t test]. Similarly, the average histoscore for PDAC samples was 153 (range, 0-300), while for normal ducts it was 0.52 (range, 0-18) [p <.0001, Independent sample t test]. Finally, with a cut-off of 12.5% positivity of cells and at least weak staining, a sensitivity of 83.9% and specificity of 100% was achieved. CONCLUSION The results of this study with 100% specificity and almost 84% sensitivity demonstrate that KOC expression is essentially restricted to PDAC in tissue samples. Thus it has the potential to increase the diagnostic accuracy of cytological samples of PDAC, but further studies on cytologic samples of PDAC and normal ducts are required. J Pathol Inform. 2011; 2: 43c. > Sub-article 2

African Journal of Urology, Apr 18, 2006