M. Degré - Academia.edu (original) (raw)
Papers by M. Degré
Acta Pathologica Microbiologica Scandinavica Series B: Microbiology, 2009
Acta Pathologica Microbiologica Scandinavica Section B Microbiology, 2009
The effects of ascorbic acid on interferon production and on the antiviral effect of interferon i... more The effects of ascorbic acid on interferon production and on the antiviral effect of interferon in cultures of human cells were investigated. Ascorbic acid enhanced the interferon levels produced by human embryo skin and human embryo lung fibroblasts, induced by Newcastle disease virus and by polyinosinic-polycytidylic acid. The same concentrations of ascorbic acid had no effect on interferon production in two lymphoblastoid cell lines induced by Sendai virus. Leucocyte interferon assayed in lung fibroblasts titrated 0.2-0.3 log10 units higher in the presence of 5 mug ascorbic acid than in the absence of the latter.
The Biology of the Interferon System 1986, 1987
The etiology of psoriasis is yet to be solved. However, there is a large body of evidence pointin... more The etiology of psoriasis is yet to be solved. However, there is a large body of evidence pointing to involvement of immune mechanisms in the pathogenesis of this disease. Thus, several authors reported presence of auto-antibodies against different structures in the skin, as stratum cornea antigen, basal nuclei and proliferating cells in psoriatic lesions. Impaired skin reaction of delayed-hypersensisivity type indicated that cell mediated immunity functions are also involved. Finally, several groups of investigators reported a large variety of alterations in mononuclear cell parameters. A depletion of T cells in peripheral blood and suppressor cell deffects were observed by some authors, but not by others. Altered monocyte/macrophage activity, measured by clearence of 51CR-labelled erythrocytes, increased chemotaxis and elevated phagocytic and bactericidal activities, are also common findings.
Journal of Interferon Research, 1990
Previous studies have shown that interferons (IFNs) specifically interact with a number of cells ... more Previous studies have shown that interferons (IFNs) specifically interact with a number of cells cultured in vitro and reduce the invasiveness of facultatively intracellular bacteria. IFN treatment also reduced the internalization of vesicular stomatitis virus (VSV) in cell cultures. Here we show that the anti-invasive effect of IFN on bacteria is eliminated in an L-cell variant where its effect on the uptake of vesicular stomatitis virus is lost. The data strongly suggest that the anti-invasive effect of IFN is mediated through inhibition of endocytosis.
Tidsskrift for den Norske lægeforening : tidsskrift for praktisk medicin, ny række, Jan 20, 1974
Tidsskrift for den Norske lægeforening : tidsskrift for praktisk medicin, ny række, Jan 10, 1991
Journal of biological regulators and homeostatic agents
The effect of interferon treatment on interaction of Shigella flexneri with in vitro cultured cel... more The effect of interferon treatment on interaction of Shigella flexneri with in vitro cultured cells was investigated. Pretreatment of HEp-2 cells with human interferons had no effect on the susceptibility of cells to S. flexneri, measured by invasiveness and adhesiveness. Human leukocyte interferon and human recombinant interferon-alpha-A reduced adhesiveness, intracellular multiplication and invasiveness of S. flexneri in HEp-2 cells preinfected with coxsackie B1 virus. Also non-receptor mediated-phagocytosis was reduced by interferon treatment in virus infected cells. The interferon effects were dependent on continuous protein synthesis, because they were not expressed when cycloheximide or abrin was added to the virus infected cell cultures. No effect of interferon was detected on intracellular content of Na+ or K+, Na(+)-K+ activated ATPase activity or cytoplasma membrane polarity, in virus infected or control cell cultures. The interferon effect on bacterial invasiveness seems ...
Journal of Medical Microbiology, 1969
Clinical Microbiology and Infection, 2002
Chemotherapy, 1993
The effect of acyclovir (ACV) treatment on selected functions of human blood-derived macrophages ... more The effect of acyclovir (ACV) treatment on selected functions of human blood-derived macrophages was examined. ACV was not cytotoxic when applied in a wide range of concentrations. Only minor effects on macrophage functions were observed when cells were treated with therapeutic concentrations of ACV:phagocytosis and the production of interferon and tumor necrosis factor were slightly enhanced, while the production of lysozyme was reduced, in a dose-dependent manner. Interferon production was also reduced in the presence of high concentrations of ACV.
Clinical Microbiology and Infection, 2001
Objective To evaluate the performance of the recently introduced method based on detection of hum... more Objective To evaluate the performance of the recently introduced method based on detection of human cytomegalovirus (HCMV) pp67 mRNA in blood by the nucleic acid sequence-based ampli®cation (NucliSens), in comparison to semiquantitative detection of pp65 HCMV antigen in white blood cells, in relation to development of clinical HCMV disease. Methods Thirty patients, recipients of renal transplants, were monitored prospectively for the presence of pp67 mRNA, the presence and level of pp65 antigenemia, IgG and IgM antibodies, and the development of clinical HCMV disease. A total of 148 samples were examined during the observation period. Results Twenty-®ve samples were positive for pp67-mRNA and 45 samples contained at least one pp65 positive cell, with 68% agreement between the two assays. Both assays predicted correctly the development of clinical disease in ®ve patients, giving a sensitivity of 100%. However, the speci®city of the pp67-mRNA test was 72%, and of the pp65 antigenemia test from 20 to 64%, depending on the level of antigenemia chosen for cutoff. pp67-RNA appeared somewhat earlier than pp65 antigenemia, and responded earlier to treatment. Sero-conversion and appearance of IgM antibodies were of very little clinical value. Conclusion Both the pp67-mRNA and the pp65 antigenemia assay predicted correctly the development of clinical HCMV disease in renal transplant recipients. However, the speci®city of both tests with respect to development of HCMV disease, especially the pp65 antigen test was moderate. Signi®cantly positive tests not necessarily prove the development of clinical disease. Testing for pp67-mRNA may improve the diagnosis and management of HCMV disease in renal transplant patients.
Acta Pathologica Microbiologica Scandinavica Series B Microbiology, 1984
The influence of infection of HEp-2 cells with Coxsackie B 1 virus on the invasiveness of Salmone... more The influence of infection of HEp-2 cells with Coxsackie B 1 virus on the invasiveness of Salmonella typhimurium has been studied. The bacterial invasiveness was significantly increased in the cell cultures incubated with virus before bacterial inoculation. This effect was a function of time after introduction of virus into the cell cultures and the concentration of virus. The increase in bacterial invasiveness was observed before production of infectious virus particles and before development of cytopathogenic effect was evident. Two strains of non-invasive Escherichia coli did not show invasiveness after the virus treatment of the cells. The results indicate a specific mechanism for the interaction.
Acta Pathologica Microbiologica Scandinavica Series B Microbiology, 1983
A murine osteosarcoma (OGS) cell line was permanently infected with Newcastle disease virus (NDV)... more A murine osteosarcoma (OGS) cell line was permanently infected with Newcastle disease virus (NDV). The presence of NDV in the subsequent passages was demonstrated by haemadsorption and by immunofluorescence. Compared to the uninfected OGS line the persistently infected cells had a slightly reduced growth rate and they had a reduced sensitivity to several viruses, shown by several different methods. Interferon sensitivity was considerably reduced in the OGS-NDV cells, both the antiviral activity and the cell multiplication inhibitory activity.
Aviation Space and Environmental Medicine, 2000
The aim of the study was to detect the prevalence of antibodies against hepatitis A, B, C, and E ... more The aim of the study was to detect the prevalence of antibodies against hepatitis A, B, C, and E viruses in flying airline personnel, and to determine the necessity of hepatitis A vaccination to prevent such infections related to occupational exposure. Antibodies against hepatitis A (HAV), B (HBV), C (HVC), and E (HEV) were tested for using standard enzyme immunoassay in airline personnel, 208 flying personnel, 199 ground crew, and 204 employees from companies not involved in travel activities. Prevalence of antibodies against HAV was less than 5% in each group, and there was no significant difference between the three groups. Prevalence of antibodies against HEV was significantly higher in flying personnel (3.4%) than in the control groups. Prevalence of antibodies against HBV and HCV was low in each of the three groups and there were no differences between the three groups. Infection with HAV, HBV and HCV does not seem to represent an occupational hazard to flying personnel. It is...
Tidsskrift for Den norske legeforening, Feb 28, 1973
Acta Pathologica, Microbiologica et Immunologica Scandinavica (APMIS), 1995
Coxsackie B1 virus infection enhances the susceptibility of in vitro cultured HEp-2 cells to inva... more Coxsackie B1 virus infection enhances the susceptibility of in vitro cultured HEp-2 cells to invasiveness by Shigella flexneri. We have studied the effect of viral infection on two phases of the invasiveness. Only a minor part was mediated by enhanced bacterial adherence to the cells, and the intracellular multiplication was unaffected by the virus. Enhanced adherence was not dependent on the presence of the gene product of the 140 Md virulence associated plasmid. Our data indicate that enhanced invasiveness induced by viral infection is mediated by an effect on other phases of the invasiveness.
Blood, Oct 1, 1996
Recently, considerable interest has arisen as to use cord blood (CB) as a source of hematopoietic... more Recently, considerable interest has arisen as to use cord blood (CB) as a source of hematopoietic stem cells for allogenic transplantation when bone marrow (BM) from a familial HLA-matched donor is not available. Because human cytomegalovirus (HCMV) has been shown to inhibit the proliferation of BM progenitors in vitro, it was important to examine whether similar effect could be observed in HCMV-infected CB cells. Therefore, the effect of HCMV challenge on the proliferation of myeloid progenitors from BM and CB was compared using both mononuclear cells (MNC) and purified CD34+ cells. A clinical isolate of HCMV inhibited the colony formation of myeloid BM progenitors responsive to granulocyte-macrophage colony-stimulating factor (CSF), granulocyte-CSF, macrophage-CSF, interleukin-3 (IL-3) and the combination of IL-3 and stem cell factor (SCF). In contrast, colony growth of CB progenitors was not affected. In addition, HCMV inhibited directly the growth of purified BM CD34+ cells resp...
Journal of the Oslo city hospitals, 1966
Acta pathologica et microbiologica Scandinavica. Section B: Microbiology and immunology, 1973
A standard bacterial vaccine (SBV) induced interferon (IF) production and reduced the mortality d... more A standard bacterial vaccine (SBV) induced interferon (IF) production and reduced the mortality due to influenza A2 virus infection in germfree mice. These effects were quantitatively comparable in conventional and germfree mice. The length of survival was somewhat shorter in conventional mice. It is concluded, that SBV exerts a direct effect on the viral infection probably partly mediated by IF induction. The role of resident bacterial flora is discussed.
Acta Pathologica Microbiologica Scandinavica Series B: Microbiology, 2009
Acta Pathologica Microbiologica Scandinavica Section B Microbiology, 2009
The effects of ascorbic acid on interferon production and on the antiviral effect of interferon i... more The effects of ascorbic acid on interferon production and on the antiviral effect of interferon in cultures of human cells were investigated. Ascorbic acid enhanced the interferon levels produced by human embryo skin and human embryo lung fibroblasts, induced by Newcastle disease virus and by polyinosinic-polycytidylic acid. The same concentrations of ascorbic acid had no effect on interferon production in two lymphoblastoid cell lines induced by Sendai virus. Leucocyte interferon assayed in lung fibroblasts titrated 0.2-0.3 log10 units higher in the presence of 5 mug ascorbic acid than in the absence of the latter.
The Biology of the Interferon System 1986, 1987
The etiology of psoriasis is yet to be solved. However, there is a large body of evidence pointin... more The etiology of psoriasis is yet to be solved. However, there is a large body of evidence pointing to involvement of immune mechanisms in the pathogenesis of this disease. Thus, several authors reported presence of auto-antibodies against different structures in the skin, as stratum cornea antigen, basal nuclei and proliferating cells in psoriatic lesions. Impaired skin reaction of delayed-hypersensisivity type indicated that cell mediated immunity functions are also involved. Finally, several groups of investigators reported a large variety of alterations in mononuclear cell parameters. A depletion of T cells in peripheral blood and suppressor cell deffects were observed by some authors, but not by others. Altered monocyte/macrophage activity, measured by clearence of 51CR-labelled erythrocytes, increased chemotaxis and elevated phagocytic and bactericidal activities, are also common findings.
Journal of Interferon Research, 1990
Previous studies have shown that interferons (IFNs) specifically interact with a number of cells ... more Previous studies have shown that interferons (IFNs) specifically interact with a number of cells cultured in vitro and reduce the invasiveness of facultatively intracellular bacteria. IFN treatment also reduced the internalization of vesicular stomatitis virus (VSV) in cell cultures. Here we show that the anti-invasive effect of IFN on bacteria is eliminated in an L-cell variant where its effect on the uptake of vesicular stomatitis virus is lost. The data strongly suggest that the anti-invasive effect of IFN is mediated through inhibition of endocytosis.
Tidsskrift for den Norske lægeforening : tidsskrift for praktisk medicin, ny række, Jan 20, 1974
Tidsskrift for den Norske lægeforening : tidsskrift for praktisk medicin, ny række, Jan 10, 1991
Journal of biological regulators and homeostatic agents
The effect of interferon treatment on interaction of Shigella flexneri with in vitro cultured cel... more The effect of interferon treatment on interaction of Shigella flexneri with in vitro cultured cells was investigated. Pretreatment of HEp-2 cells with human interferons had no effect on the susceptibility of cells to S. flexneri, measured by invasiveness and adhesiveness. Human leukocyte interferon and human recombinant interferon-alpha-A reduced adhesiveness, intracellular multiplication and invasiveness of S. flexneri in HEp-2 cells preinfected with coxsackie B1 virus. Also non-receptor mediated-phagocytosis was reduced by interferon treatment in virus infected cells. The interferon effects were dependent on continuous protein synthesis, because they were not expressed when cycloheximide or abrin was added to the virus infected cell cultures. No effect of interferon was detected on intracellular content of Na+ or K+, Na(+)-K+ activated ATPase activity or cytoplasma membrane polarity, in virus infected or control cell cultures. The interferon effect on bacterial invasiveness seems ...
Journal of Medical Microbiology, 1969
Clinical Microbiology and Infection, 2002
Chemotherapy, 1993
The effect of acyclovir (ACV) treatment on selected functions of human blood-derived macrophages ... more The effect of acyclovir (ACV) treatment on selected functions of human blood-derived macrophages was examined. ACV was not cytotoxic when applied in a wide range of concentrations. Only minor effects on macrophage functions were observed when cells were treated with therapeutic concentrations of ACV:phagocytosis and the production of interferon and tumor necrosis factor were slightly enhanced, while the production of lysozyme was reduced, in a dose-dependent manner. Interferon production was also reduced in the presence of high concentrations of ACV.
Clinical Microbiology and Infection, 2001
Objective To evaluate the performance of the recently introduced method based on detection of hum... more Objective To evaluate the performance of the recently introduced method based on detection of human cytomegalovirus (HCMV) pp67 mRNA in blood by the nucleic acid sequence-based ampli®cation (NucliSens), in comparison to semiquantitative detection of pp65 HCMV antigen in white blood cells, in relation to development of clinical HCMV disease. Methods Thirty patients, recipients of renal transplants, were monitored prospectively for the presence of pp67 mRNA, the presence and level of pp65 antigenemia, IgG and IgM antibodies, and the development of clinical HCMV disease. A total of 148 samples were examined during the observation period. Results Twenty-®ve samples were positive for pp67-mRNA and 45 samples contained at least one pp65 positive cell, with 68% agreement between the two assays. Both assays predicted correctly the development of clinical disease in ®ve patients, giving a sensitivity of 100%. However, the speci®city of the pp67-mRNA test was 72%, and of the pp65 antigenemia test from 20 to 64%, depending on the level of antigenemia chosen for cutoff. pp67-RNA appeared somewhat earlier than pp65 antigenemia, and responded earlier to treatment. Sero-conversion and appearance of IgM antibodies were of very little clinical value. Conclusion Both the pp67-mRNA and the pp65 antigenemia assay predicted correctly the development of clinical HCMV disease in renal transplant recipients. However, the speci®city of both tests with respect to development of HCMV disease, especially the pp65 antigen test was moderate. Signi®cantly positive tests not necessarily prove the development of clinical disease. Testing for pp67-mRNA may improve the diagnosis and management of HCMV disease in renal transplant patients.
Acta Pathologica Microbiologica Scandinavica Series B Microbiology, 1984
The influence of infection of HEp-2 cells with Coxsackie B 1 virus on the invasiveness of Salmone... more The influence of infection of HEp-2 cells with Coxsackie B 1 virus on the invasiveness of Salmonella typhimurium has been studied. The bacterial invasiveness was significantly increased in the cell cultures incubated with virus before bacterial inoculation. This effect was a function of time after introduction of virus into the cell cultures and the concentration of virus. The increase in bacterial invasiveness was observed before production of infectious virus particles and before development of cytopathogenic effect was evident. Two strains of non-invasive Escherichia coli did not show invasiveness after the virus treatment of the cells. The results indicate a specific mechanism for the interaction.
Acta Pathologica Microbiologica Scandinavica Series B Microbiology, 1983
A murine osteosarcoma (OGS) cell line was permanently infected with Newcastle disease virus (NDV)... more A murine osteosarcoma (OGS) cell line was permanently infected with Newcastle disease virus (NDV). The presence of NDV in the subsequent passages was demonstrated by haemadsorption and by immunofluorescence. Compared to the uninfected OGS line the persistently infected cells had a slightly reduced growth rate and they had a reduced sensitivity to several viruses, shown by several different methods. Interferon sensitivity was considerably reduced in the OGS-NDV cells, both the antiviral activity and the cell multiplication inhibitory activity.
Aviation Space and Environmental Medicine, 2000
The aim of the study was to detect the prevalence of antibodies against hepatitis A, B, C, and E ... more The aim of the study was to detect the prevalence of antibodies against hepatitis A, B, C, and E viruses in flying airline personnel, and to determine the necessity of hepatitis A vaccination to prevent such infections related to occupational exposure. Antibodies against hepatitis A (HAV), B (HBV), C (HVC), and E (HEV) were tested for using standard enzyme immunoassay in airline personnel, 208 flying personnel, 199 ground crew, and 204 employees from companies not involved in travel activities. Prevalence of antibodies against HAV was less than 5% in each group, and there was no significant difference between the three groups. Prevalence of antibodies against HEV was significantly higher in flying personnel (3.4%) than in the control groups. Prevalence of antibodies against HBV and HCV was low in each of the three groups and there were no differences between the three groups. Infection with HAV, HBV and HCV does not seem to represent an occupational hazard to flying personnel. It is...
Tidsskrift for Den norske legeforening, Feb 28, 1973
Acta Pathologica, Microbiologica et Immunologica Scandinavica (APMIS), 1995
Coxsackie B1 virus infection enhances the susceptibility of in vitro cultured HEp-2 cells to inva... more Coxsackie B1 virus infection enhances the susceptibility of in vitro cultured HEp-2 cells to invasiveness by Shigella flexneri. We have studied the effect of viral infection on two phases of the invasiveness. Only a minor part was mediated by enhanced bacterial adherence to the cells, and the intracellular multiplication was unaffected by the virus. Enhanced adherence was not dependent on the presence of the gene product of the 140 Md virulence associated plasmid. Our data indicate that enhanced invasiveness induced by viral infection is mediated by an effect on other phases of the invasiveness.
Blood, Oct 1, 1996
Recently, considerable interest has arisen as to use cord blood (CB) as a source of hematopoietic... more Recently, considerable interest has arisen as to use cord blood (CB) as a source of hematopoietic stem cells for allogenic transplantation when bone marrow (BM) from a familial HLA-matched donor is not available. Because human cytomegalovirus (HCMV) has been shown to inhibit the proliferation of BM progenitors in vitro, it was important to examine whether similar effect could be observed in HCMV-infected CB cells. Therefore, the effect of HCMV challenge on the proliferation of myeloid progenitors from BM and CB was compared using both mononuclear cells (MNC) and purified CD34+ cells. A clinical isolate of HCMV inhibited the colony formation of myeloid BM progenitors responsive to granulocyte-macrophage colony-stimulating factor (CSF), granulocyte-CSF, macrophage-CSF, interleukin-3 (IL-3) and the combination of IL-3 and stem cell factor (SCF). In contrast, colony growth of CB progenitors was not affected. In addition, HCMV inhibited directly the growth of purified BM CD34+ cells resp...
Journal of the Oslo city hospitals, 1966
Acta pathologica et microbiologica Scandinavica. Section B: Microbiology and immunology, 1973
A standard bacterial vaccine (SBV) induced interferon (IF) production and reduced the mortality d... more A standard bacterial vaccine (SBV) induced interferon (IF) production and reduced the mortality due to influenza A2 virus infection in germfree mice. These effects were quantitatively comparable in conventional and germfree mice. The length of survival was somewhat shorter in conventional mice. It is concluded, that SBV exerts a direct effect on the viral infection probably partly mediated by IF induction. The role of resident bacterial flora is discussed.