M. Kadin - Academia.edu (original) (raw)

Papers by M. Kadin

Research paper thumbnail of Primary Cutaneous Anaplastic Large-cell Lymphoma

American Journal of Clinical Oncology, 2013

Research paper thumbnail of Peripheral T Cell Lymphoma, NOS

Research paper thumbnail of T gamma cells: a missing link between malignant histiocytosis and T cell leukemia-lymphoma?

Human pathology, 1981

At least nine patient~ have been reported to develop malignant histiocytosis or a histiocytic med... more At least nine patient~ have been reported to develop malignant histiocytosis or a histiocytic medullary reticulosis-like s y n d r o m e within a few monttts after their presentation with acute lymphocytic leukemia. ~-s In all three of these cases studied for surface markers the leukemic cells were found to be T cells, although none of these patients had a mediastinal mass that commonly accompanies acute T cell leukemia. Each of these young male patients had hepatosplenotnegaly, only mild o r no l y m p h a d enopathy, and fever. A m o r e recent case o f histiocytic medullary reticulosis has been r e p o r t e d in a two year old boy with acute lymphocytic leukemia that was also probably of the T cell type. Tiffs patient differs from previous ones in having a mediastinal mass as well as hepatosplenomegaly. 9 Explanations for malignant histiocytosis or histiocytic medullary reticulosis developing rapidly after the onset of acute lymphocytic leukemia in the same patient included the following: a second malignant tumor caused by therapy for the first disorder, a chance relationship between the two disorders, a mistaken diagnosis of one or both disorders, and a conmmn stem cell origin for both lymphocytic leukemia and histiocytosis.

Research paper thumbnail of Eosinophils are the major source of transforming growth factor-beta 1 in nodular sclerosing Hodgkin's disease

The American journal of pathology, 1993

Transforming growth factor-beta 1 (TGF-beta 1) is a multifunctional cytokine which promotes fibro... more Transforming growth factor-beta 1 (TGF-beta 1) is a multifunctional cytokine which promotes fibroblast growth and collagen synthesis, but suppresses growth and differentiation of immune lymphocytes and killer cells. Immunohistochemical detection of TGF-beta 1 in Hodgkin's disease (HD) has been shown to correlate with the histologic feature of nodular sclerosis, which is associated with a favorable prognosis (American Journal of Pathology 1990, 136:1209). In that study, TGF-beta 1 was localized mainly at the margins of broad collagen bands (presumably sites of new collagen synthesis) and in areas containing numerous Hodgkin/Reed-Sternberg cells (H/RS). In these areas, TGF-beta 1 protein was found on the membrane and occasionally within the cytoplasm of H/RS cells. To determine whether TGF-beta 1 is synthesized by H/RS cells or secondarily bound to their membrane and sometimes internalized, we performed in situ hybridization (ISH) using 1.5 Kb 35S-labeled anti-sense and sense RNA ...

Research paper thumbnail of Molecular diagnosis of early cutaneous T cell lymphoma

Research paper thumbnail of Surface Characteristics of Hodgkin's Cells

Research paper thumbnail of Cutaneous T cell lymphoma with suppressor/cytotoxic (CD8) phenotype: Identification of rapidly progressive and chronic subtypes

Journal of the American Academy of Dermatology, 1990

We identified nine patients with cutaneous T cell lymphoma in whom the neoplastic cells expressed... more We identified nine patients with cutaneous T cell lymphoma in whom the neoplastic cells expressed the COg (T8) suppressor T cell phenotype instead of the more common CD4 (T4) helper T cell phenotype. Five had rapidly progressive disease characterized by distinctive papulonodular skin lesions (four patients), involvement of palms or soles (four patients) or oral cavity (two patients), and poor response to standard topical therapy (four patients). Histologic examination showed extensive epidermotropism often associated with pagetoid fea· tures. Immunoperoxidase studies revealed a novel aberrant T cell phenotype characterized by lack of expression of CD4 and CD2 (Tll) but positive staining for CD3 (D) and CD7 (3Al). In contrast, the neoplastic cells from four patients with clinically more chronic CD8+ cutaneous T cell lymphoma, although also commonly epidermotropic, had a different aberrant T cell phenotype similar to that often seen in CD4+ mycosis fungoides; that is, there was lack of expression of CD7 but a positive reaction to staining for C02. In two cases the tumor cells acquired the CD7 antigen or lost the CD2 antigen with progression of the disease. Two cases were analyzed with Southern blotting and both showed rearranged DNA bands that confirmed the presence of clonal populations of T cells. Our findings suggest the following:

Research paper thumbnail of Glucocorticoid receptors in normal human eosinophils: comparison with neutrophils

Journal of Allergy and Clinical Immunology, 1981

Saturation analysis with [3H]-dexamethasone was employed to measure glucocorticoid binding in pkj... more Saturation analysis with [3H]-dexamethasone was employed to measure glucocorticoid binding in pkjied preparations of human eosinophils and neutrophils. Eosinophils contained 10.8 + 1.3 x 10" high-a@@ receptor sites per cell, with a dissociation constant (Kd) of 15.3 +-0.6 nM dexamethasone. Cortisol was capable of competing with [3H]-dexamethasone in the binding reaction, whereas progesterone, estradiol, estriol, and testosterone were less effective. Saturable glucocorticoid binding in neutrophils had a Kd of 17.7 2 0.8 nh4 dexamethasone with II .I k 0.8 X IO3 sites per cell ana' displayed similar steroid spec$city. These data indicate that normal human eosinophils have glucocorticoid receptors with characteristics similar to those in neutrophils and that in these cells ligand-receptor interaction can occur at physiologic glucocorticoid concentrations. Furthermore, these results suggest that certain glucocorticoid effects on eosinophils and neutrophils may be mediated through spectjic receptors. (J ALLERGY CLIN IMUUNOL 68:212, 1981.) Glucocorticoids are steroid hormones with potent anti-inflammatory effects. For this reason they are used extensively in the treatment of allergic, inflammatory, and autoimmune disorders such as asthma, rheumatoid arthritis, systemic lupus erythematosus, ulcerative colitis, and many dermatologic diseases. Eosinophils and neutrophils, long known to be responsive to glucocorticoid hormones, play a prominent role in many of these inflammatory and hypersensitivity diseases. The generally accepted model of glucocorticoid action involves hormone binding to specific intracellular cytoplasmic proteins,

Research paper thumbnail of Anti-CD30 (BER-H2) immunotoxins containing the type-1 ribosome-inactivating proteins momordin and PAP-S (pokeweed antiviral protein from seeds) display powerful antitumour activity against CD30 + tumour cells in vitro and in SCID mice

British Journal of Haematology, 1996

The anti-CD30 immunotoxin (IT) Ber-H2/saporin is effective in patients with refractory Hodgkin&am... more The anti-CD30 immunotoxin (IT) Ber-H2/saporin is effective in patients with refractory Hodgkin's disease. However, responses are short and partial, one of the main reasons being the inability to repeat IT doses because of formation of human antibodies against the murine antibody and/or the toxin. To overcome this problem, we constructed two new anti-CD30 ITs by covalently linking the mouse monoclonal antibody Ber-H2 to the type 1 ribosome-inactivating proteins (RIPs) momordin (MOM) and pokeweed antiviral protein from seeds (PAP-S), which do not cross-react with each other or with saporin. Both ITs inhibited protein synthesis by Hodgkin's disease and anaplastic large-cell lymphoma (ALCL)-derived CD30+ target cell lines with a very high efficiency (IC50 ranging from < 5 x 10(-13) M to 2.75 x 10(-11) M, as RIP). In a SCID mouse model of xenografted CD30+ human ALCL, a 3d treatment with non-toxin doses of Ber-H2/MOM (50%LD50), started 24 h after transplantation, prevented tumour development in about 40% of the animals and significantly delayed tumour growth rate in the others. Main toxicity signs in mice and rabbits were dose-related increase of serum transaminases (AST and ALT) and creatine phosphokinase (CPK). LD50 (as RIP) in Swiss mice was 7 mg/kg for Ber-H2/MOM and 0.45 mg/kg for Ber-H2/PAP-S. Sequential administration of two anti-CD30 ITs (Ber-H2/MOM and Ber-H2/saporin) was well tolerated and did not result in formation of antibodies cross-reacting and with the two plant toxins. The results presented in this paper suggest that in the future, sequential administration of anti-CD30 humanized antibodies linked to antigenically distinct type 1 RIPs (saporin, MOM, PAP-S) should be feasible.

Research paper thumbnail of Interleukin-9 expression in human malignant lymphomas: unique association with Hodgkin's disease and large cell anaplastic lymphoma

Blood, 1991

To test the possibility that interleukin-9 (IL-9), the human homologue of the mouse T-cell growth... more To test the possibility that interleukin-9 (IL-9), the human homologue of the mouse T-cell growth factor P40, may be involved in the pathogenesis of human lymphomas, we examined IL-9 expression in a variety of tumors both by Northern blot analysis and by in situ hybridization. Of 18 B-cell non-Hodgkin's lymphomas and 11 peripheral T-cell lymphomas, none expressed IL-9 message. By contrast, IL-9 message was found in two of six cases of large cell anaplastic lymphoma (LCAL) and in 6 of 13 cases of Hodgkin's disease (HD). In HD the strongest signals were observed in Hodgkin (H) and Sternberg-Reed (SR) cells, but IL-9 mRNA was also detected in small lymphocytic cells. A search for IL-9 message in a panel of 20 cell lines derived both from hematopoietic and nonhematopoietic tumors confirmed the unique association of IL-9 expression with HD and LCAL in as much as the only two cell lines with IL-9 message were derived from cases of HD and LCAL. These results suggest that IL-9 is no...

Research paper thumbnail of Immunofluorescent method for positive identification of null-cell type acute lymphocytic leukemias: use of heterologous antiserum

Blood, 1977

Acute lymphoblastic leukemia (ALL) patients could be subclassified into two groups depending on w... more Acute lymphoblastic leukemia (ALL) patients could be subclassified into two groups depending on whether or not their leukemia cells expressed a B-lymphocyte antigen. The antigen was detected by an indirect immunofluorescence test using rabbit antisera. In the positive group, consisting of 26 of 32 patients, the leukemia cells were of the "null"-cell type, i.e., they did not appear to express currently recognized T- and -b-cell markers. Absroption studies indicated that the positive null-cell group expressed a common antigen which was not expressed on the negative group. Of the 6 negative cases,, 5 expressed complement receptors and 3 expressed T-cell markers. The negative group was also characterized by high white cell counts and the pressence of a mediastinal mass.

Research paper thumbnail of Comparison of treatment regimens for pediatric lymphoblastic non-Hodgkin's lymphoma: a Childrens Cancer Group study

Journal of clinical oncology : official journal of the American Society of Clinical Oncology, 1995

Patients with lymphoblastic non-Hodgkin's lymphoma (LB NHL) were randomized to treatment with... more Patients with lymphoblastic non-Hodgkin's lymphoma (LB NHL) were randomized to treatment with either modified LSA2L2 or ADCOMP, which added daunorubicin (DAUN) and asparaginase (L-ASP) to the methotrexate (MTX), cyclophosphamide (CYT), vincristine (VCR), and prednisone (PRED) (COMP) regimen, in a clinical trial to determine the relative effectiveness and toxicity of the two regimens. Patients with LB NHL were eligible for this randomized study if they were less than 22 years of age at diagnosis and had < or = 25% blasts in the bone marrow. Of 307 patients registered, 281 were fully eligible and assessable. Patients were stratified by extent of disease at diagnosis. The 5-year event-free survival (EFS) rate for patients with localized disease was 84%, and for patients with disseminated disease, 67%. There were four relapses in 28 patients with localized disease. Two hundred six patients had mediastinal primary tumors and despite local radiation, 34 of 63 failures in these pati...

Research paper thumbnail of Smog eye irritation: effect of air pollution on the tear protein pattern

Ophthalmologica. Journal international d'ophtalmologie. International journal of ophthalmology. Zeitschrift für Augenheilkunde, 1969

Research paper thumbnail of 613 Acquired Immunodeficiency Syndrome in the United States: An Analysis of Cases Outside High-Incidence Groups

Research paper thumbnail of Cutaneous T-cell lymphoma with porokeratosis-like lesions

Journal of the American Academy of Dermatology, 1992

We describe two black patients who had widespread, sharply demarcated, round, hyperpigmented or h... more We describe two black patients who had widespread, sharply demarcated, round, hyperpigmented or hypopigmented macules that clinically resembled disseminated superficial actinic porokeratosis (DSAP). One patient was known to have cutaneous T-cell lymphoma (CTCL), and the other patient had only the DSAP-like lesions. Histopathologie and immunohistochemical studies of the DSAP-like lesions revealed characteristic features of patch-phase CTCL. This unusual variant of patch-phase CTCL should be differentiated from DSAP and the hypopigmented variant of CTCL. (J AM ACAD DERMATOL 1992;27:327-30.)

Research paper thumbnail of Complete resolution of Kaposi's sarcoma with systemic etretinate therapy in a patient with mycosis fungoides

Journal of the American Academy of Dermatology, 1989

1. J Am Acad Dermatol. 1989 Jun;20(6):1123-4. Complete resolution of Kaposi's sarcoma with s... more 1. J Am Acad Dermatol. 1989 Jun;20(6):1123-4. Complete resolution of Kaposi's sarcoma with systemic etretinate therapy in a patient with mycosis fungoides. Sharpe RJ, Kadin ME, Harmon DC, Imber MJ, Anderson RR. PMID: 2754062 [PubMed - indexed for MEDLINE]. ...

Research paper thumbnail of Extracorporeal photopheresis in psoriasis vulgaris: Clinical and immunologic observations

Journal of the American Academy of Dermatology, 1990

10. Brown MD, EllisC,Voorhees JJ. Cyclosporinc A: a review of its dermatologic applications. Semi... more 10. Brown MD, EllisC,Voorhees JJ. Cyclosporinc A: a review of its dermatologic applications. Semin Dermatol 1987; 6:2-9. II. Cunliffe WJ. Pemphigus Ioliaceus and response to cyclosporin. Br J Dermatol 1987; 117(suppl 32): 114-6. 12. Palestine AG, Nussemblatt RB, Chance. Sideeffects of systemic cyclosporin in patients not undergoing transplantation. Am J Med 1984;77:652-6. 13. Boungneres PF, Cavel lC, Castano L, et al. Factors associated with early remission of type I diabetes in children Cyclosporine therapy in pemphigus ' . u!,:;arjs treated with cyclosporine. N Engl

Research paper thumbnail of Cutaneous manifestations of postthymic T cell malignancies: Description of five clinicopathologic subtypes

Journal of the American Academy of Dermatology, 1990

This study was undertaken to identify the cutaneous manifestations among different prognostic sub... more This study was undertaken to identify the cutaneous manifestations among different prognostic subgroups of postthymic T cell malignancies. Cutaneous involvement was demonstrated in 43 of 88 cases. We recognized five clinicopathologic subtypes: typeI, classical cu-taneousT celllymphoma (Cf'Cl.) or mycosis fungoides, sixcases; type II, primary large cell type CTCL, Ki-l antigen (Ki-l + or Ki-T"), seven cases; typeIII, primary angioinvasive T celllymphoma, three cases; type IV,human T-Iymphotropic virus typeI (HTLV-I+) adult T cellleukemia/lymphoma (ATL), eightcases; type V,secondary cutaneous involvement by peripheralT celllymphoma (PTL), 19cases. Primary CTCL and ATL tendto involve papillary dermiswith or withoutepidermotropism, whereas PTL and angioinvasive T cell lymphoma predominantly affectskin adnexae, vessels, and subcutis. Cutaneous lesions in type V PTL are heterogeneous and may be confused with panniculitis, vasculitis, or an eczematous eruption. ClassicCTCL, Ki-l + lymphoma, and angioinvasive T cell lymphoma have a chroniccourse, whereas ATL, Ki-Ir large celllymphoma, andP1Lareclinically aggressive.

Research paper thumbnail of ULTRASTRUCTURAL LOCALIZATION OF IMMUNOGLOBULINS IN BULLOUS PEMPHIGOID SKIN. Employment of a New Peroxidase - Antiperoxidase Multistep Method

Journal of Investigative Dermatology, 1975

Abstract A multistep immunochemical method was developed for light and electron microscopy utiliz... more Abstract A multistep immunochemical method was developed for light and electron microscopy utilizing horseradish peroxidase as an immunologically bound marker. The method was applied to studies of in vivo bound IgG in bullous pemphigoid skin and of ...

Research paper thumbnail of Molecular evidence for a clonal relationship between lymphomatoid papulosis and Ki-1 positive large cell anaplastic lymphoma

Journal of Dermatological Science, 1993

Currently, considerable controversy surrounds questions about the clonal evolution of lymphomas i... more Currently, considerable controversy surrounds questions about the clonal evolution of lymphomas in patients with lymphomatoid papulosis. In order to analyze a possible clonal relationship between lesions of lymphomatoid papulosis and a Ki l+ large cell anaplastic lymphoma in the same patient, a highly specific molecular probe for the malignant lymphoid clone of the large cell anaplastic lymphoma was developed. As a clone specific molecular marker, highly variable junctional sequences of rearranged T-cell receptor-gamma genes were used. An oligonucleotide primer complementary to these sequences was synthesized and, using the polymerase chain reaction, clone specific DNA was detected in all lesions of lymphomatoid papulosis of the patient. These results provide evidence for a clonal relationship between lesions of lymphomatoid papulosis and large cell anaplastic lymphoma developing in the same patient.

Research paper thumbnail of Primary Cutaneous Anaplastic Large-cell Lymphoma

American Journal of Clinical Oncology, 2013

Research paper thumbnail of Peripheral T Cell Lymphoma, NOS

Research paper thumbnail of T gamma cells: a missing link between malignant histiocytosis and T cell leukemia-lymphoma?

Human pathology, 1981

At least nine patient~ have been reported to develop malignant histiocytosis or a histiocytic med... more At least nine patient~ have been reported to develop malignant histiocytosis or a histiocytic medullary reticulosis-like s y n d r o m e within a few monttts after their presentation with acute lymphocytic leukemia. ~-s In all three of these cases studied for surface markers the leukemic cells were found to be T cells, although none of these patients had a mediastinal mass that commonly accompanies acute T cell leukemia. Each of these young male patients had hepatosplenotnegaly, only mild o r no l y m p h a d enopathy, and fever. A m o r e recent case o f histiocytic medullary reticulosis has been r e p o r t e d in a two year old boy with acute lymphocytic leukemia that was also probably of the T cell type. Tiffs patient differs from previous ones in having a mediastinal mass as well as hepatosplenomegaly. 9 Explanations for malignant histiocytosis or histiocytic medullary reticulosis developing rapidly after the onset of acute lymphocytic leukemia in the same patient included the following: a second malignant tumor caused by therapy for the first disorder, a chance relationship between the two disorders, a mistaken diagnosis of one or both disorders, and a conmmn stem cell origin for both lymphocytic leukemia and histiocytosis.

Research paper thumbnail of Eosinophils are the major source of transforming growth factor-beta 1 in nodular sclerosing Hodgkin's disease

The American journal of pathology, 1993

Transforming growth factor-beta 1 (TGF-beta 1) is a multifunctional cytokine which promotes fibro... more Transforming growth factor-beta 1 (TGF-beta 1) is a multifunctional cytokine which promotes fibroblast growth and collagen synthesis, but suppresses growth and differentiation of immune lymphocytes and killer cells. Immunohistochemical detection of TGF-beta 1 in Hodgkin's disease (HD) has been shown to correlate with the histologic feature of nodular sclerosis, which is associated with a favorable prognosis (American Journal of Pathology 1990, 136:1209). In that study, TGF-beta 1 was localized mainly at the margins of broad collagen bands (presumably sites of new collagen synthesis) and in areas containing numerous Hodgkin/Reed-Sternberg cells (H/RS). In these areas, TGF-beta 1 protein was found on the membrane and occasionally within the cytoplasm of H/RS cells. To determine whether TGF-beta 1 is synthesized by H/RS cells or secondarily bound to their membrane and sometimes internalized, we performed in situ hybridization (ISH) using 1.5 Kb 35S-labeled anti-sense and sense RNA ...

Research paper thumbnail of Molecular diagnosis of early cutaneous T cell lymphoma

Research paper thumbnail of Surface Characteristics of Hodgkin's Cells

Research paper thumbnail of Cutaneous T cell lymphoma with suppressor/cytotoxic (CD8) phenotype: Identification of rapidly progressive and chronic subtypes

Journal of the American Academy of Dermatology, 1990

We identified nine patients with cutaneous T cell lymphoma in whom the neoplastic cells expressed... more We identified nine patients with cutaneous T cell lymphoma in whom the neoplastic cells expressed the COg (T8) suppressor T cell phenotype instead of the more common CD4 (T4) helper T cell phenotype. Five had rapidly progressive disease characterized by distinctive papulonodular skin lesions (four patients), involvement of palms or soles (four patients) or oral cavity (two patients), and poor response to standard topical therapy (four patients). Histologic examination showed extensive epidermotropism often associated with pagetoid fea· tures. Immunoperoxidase studies revealed a novel aberrant T cell phenotype characterized by lack of expression of CD4 and CD2 (Tll) but positive staining for CD3 (D) and CD7 (3Al). In contrast, the neoplastic cells from four patients with clinically more chronic CD8+ cutaneous T cell lymphoma, although also commonly epidermotropic, had a different aberrant T cell phenotype similar to that often seen in CD4+ mycosis fungoides; that is, there was lack of expression of CD7 but a positive reaction to staining for C02. In two cases the tumor cells acquired the CD7 antigen or lost the CD2 antigen with progression of the disease. Two cases were analyzed with Southern blotting and both showed rearranged DNA bands that confirmed the presence of clonal populations of T cells. Our findings suggest the following:

Research paper thumbnail of Glucocorticoid receptors in normal human eosinophils: comparison with neutrophils

Journal of Allergy and Clinical Immunology, 1981

Saturation analysis with [3H]-dexamethasone was employed to measure glucocorticoid binding in pkj... more Saturation analysis with [3H]-dexamethasone was employed to measure glucocorticoid binding in pkjied preparations of human eosinophils and neutrophils. Eosinophils contained 10.8 + 1.3 x 10" high-a@@ receptor sites per cell, with a dissociation constant (Kd) of 15.3 +-0.6 nM dexamethasone. Cortisol was capable of competing with [3H]-dexamethasone in the binding reaction, whereas progesterone, estradiol, estriol, and testosterone were less effective. Saturable glucocorticoid binding in neutrophils had a Kd of 17.7 2 0.8 nh4 dexamethasone with II .I k 0.8 X IO3 sites per cell ana' displayed similar steroid spec$city. These data indicate that normal human eosinophils have glucocorticoid receptors with characteristics similar to those in neutrophils and that in these cells ligand-receptor interaction can occur at physiologic glucocorticoid concentrations. Furthermore, these results suggest that certain glucocorticoid effects on eosinophils and neutrophils may be mediated through spectjic receptors. (J ALLERGY CLIN IMUUNOL 68:212, 1981.) Glucocorticoids are steroid hormones with potent anti-inflammatory effects. For this reason they are used extensively in the treatment of allergic, inflammatory, and autoimmune disorders such as asthma, rheumatoid arthritis, systemic lupus erythematosus, ulcerative colitis, and many dermatologic diseases. Eosinophils and neutrophils, long known to be responsive to glucocorticoid hormones, play a prominent role in many of these inflammatory and hypersensitivity diseases. The generally accepted model of glucocorticoid action involves hormone binding to specific intracellular cytoplasmic proteins,

Research paper thumbnail of Anti-CD30 (BER-H2) immunotoxins containing the type-1 ribosome-inactivating proteins momordin and PAP-S (pokeweed antiviral protein from seeds) display powerful antitumour activity against CD30 + tumour cells in vitro and in SCID mice

British Journal of Haematology, 1996

The anti-CD30 immunotoxin (IT) Ber-H2/saporin is effective in patients with refractory Hodgkin&am... more The anti-CD30 immunotoxin (IT) Ber-H2/saporin is effective in patients with refractory Hodgkin&amp;amp;amp;amp;#39;s disease. However, responses are short and partial, one of the main reasons being the inability to repeat IT doses because of formation of human antibodies against the murine antibody and/or the toxin. To overcome this problem, we constructed two new anti-CD30 ITs by covalently linking the mouse monoclonal antibody Ber-H2 to the type 1 ribosome-inactivating proteins (RIPs) momordin (MOM) and pokeweed antiviral protein from seeds (PAP-S), which do not cross-react with each other or with saporin. Both ITs inhibited protein synthesis by Hodgkin&amp;amp;amp;amp;#39;s disease and anaplastic large-cell lymphoma (ALCL)-derived CD30+ target cell lines with a very high efficiency (IC50 ranging from &amp;amp;amp;amp;lt; 5 x 10(-13) M to 2.75 x 10(-11) M, as RIP). In a SCID mouse model of xenografted CD30+ human ALCL, a 3d treatment with non-toxin doses of Ber-H2/MOM (50%LD50), started 24 h after transplantation, prevented tumour development in about 40% of the animals and significantly delayed tumour growth rate in the others. Main toxicity signs in mice and rabbits were dose-related increase of serum transaminases (AST and ALT) and creatine phosphokinase (CPK). LD50 (as RIP) in Swiss mice was 7 mg/kg for Ber-H2/MOM and 0.45 mg/kg for Ber-H2/PAP-S. Sequential administration of two anti-CD30 ITs (Ber-H2/MOM and Ber-H2/saporin) was well tolerated and did not result in formation of antibodies cross-reacting and with the two plant toxins. The results presented in this paper suggest that in the future, sequential administration of anti-CD30 humanized antibodies linked to antigenically distinct type 1 RIPs (saporin, MOM, PAP-S) should be feasible.

Research paper thumbnail of Interleukin-9 expression in human malignant lymphomas: unique association with Hodgkin's disease and large cell anaplastic lymphoma

Blood, 1991

To test the possibility that interleukin-9 (IL-9), the human homologue of the mouse T-cell growth... more To test the possibility that interleukin-9 (IL-9), the human homologue of the mouse T-cell growth factor P40, may be involved in the pathogenesis of human lymphomas, we examined IL-9 expression in a variety of tumors both by Northern blot analysis and by in situ hybridization. Of 18 B-cell non-Hodgkin's lymphomas and 11 peripheral T-cell lymphomas, none expressed IL-9 message. By contrast, IL-9 message was found in two of six cases of large cell anaplastic lymphoma (LCAL) and in 6 of 13 cases of Hodgkin's disease (HD). In HD the strongest signals were observed in Hodgkin (H) and Sternberg-Reed (SR) cells, but IL-9 mRNA was also detected in small lymphocytic cells. A search for IL-9 message in a panel of 20 cell lines derived both from hematopoietic and nonhematopoietic tumors confirmed the unique association of IL-9 expression with HD and LCAL in as much as the only two cell lines with IL-9 message were derived from cases of HD and LCAL. These results suggest that IL-9 is no...

Research paper thumbnail of Immunofluorescent method for positive identification of null-cell type acute lymphocytic leukemias: use of heterologous antiserum

Blood, 1977

Acute lymphoblastic leukemia (ALL) patients could be subclassified into two groups depending on w... more Acute lymphoblastic leukemia (ALL) patients could be subclassified into two groups depending on whether or not their leukemia cells expressed a B-lymphocyte antigen. The antigen was detected by an indirect immunofluorescence test using rabbit antisera. In the positive group, consisting of 26 of 32 patients, the leukemia cells were of the "null"-cell type, i.e., they did not appear to express currently recognized T- and -b-cell markers. Absroption studies indicated that the positive null-cell group expressed a common antigen which was not expressed on the negative group. Of the 6 negative cases,, 5 expressed complement receptors and 3 expressed T-cell markers. The negative group was also characterized by high white cell counts and the pressence of a mediastinal mass.

Research paper thumbnail of Comparison of treatment regimens for pediatric lymphoblastic non-Hodgkin's lymphoma: a Childrens Cancer Group study

Journal of clinical oncology : official journal of the American Society of Clinical Oncology, 1995

Patients with lymphoblastic non-Hodgkin's lymphoma (LB NHL) were randomized to treatment with... more Patients with lymphoblastic non-Hodgkin's lymphoma (LB NHL) were randomized to treatment with either modified LSA2L2 or ADCOMP, which added daunorubicin (DAUN) and asparaginase (L-ASP) to the methotrexate (MTX), cyclophosphamide (CYT), vincristine (VCR), and prednisone (PRED) (COMP) regimen, in a clinical trial to determine the relative effectiveness and toxicity of the two regimens. Patients with LB NHL were eligible for this randomized study if they were less than 22 years of age at diagnosis and had < or = 25% blasts in the bone marrow. Of 307 patients registered, 281 were fully eligible and assessable. Patients were stratified by extent of disease at diagnosis. The 5-year event-free survival (EFS) rate for patients with localized disease was 84%, and for patients with disseminated disease, 67%. There were four relapses in 28 patients with localized disease. Two hundred six patients had mediastinal primary tumors and despite local radiation, 34 of 63 failures in these pati...

Research paper thumbnail of Smog eye irritation: effect of air pollution on the tear protein pattern

Ophthalmologica. Journal international d'ophtalmologie. International journal of ophthalmology. Zeitschrift für Augenheilkunde, 1969

Research paper thumbnail of 613 Acquired Immunodeficiency Syndrome in the United States: An Analysis of Cases Outside High-Incidence Groups

Research paper thumbnail of Cutaneous T-cell lymphoma with porokeratosis-like lesions

Journal of the American Academy of Dermatology, 1992

We describe two black patients who had widespread, sharply demarcated, round, hyperpigmented or h... more We describe two black patients who had widespread, sharply demarcated, round, hyperpigmented or hypopigmented macules that clinically resembled disseminated superficial actinic porokeratosis (DSAP). One patient was known to have cutaneous T-cell lymphoma (CTCL), and the other patient had only the DSAP-like lesions. Histopathologie and immunohistochemical studies of the DSAP-like lesions revealed characteristic features of patch-phase CTCL. This unusual variant of patch-phase CTCL should be differentiated from DSAP and the hypopigmented variant of CTCL. (J AM ACAD DERMATOL 1992;27:327-30.)

Research paper thumbnail of Complete resolution of Kaposi's sarcoma with systemic etretinate therapy in a patient with mycosis fungoides

Journal of the American Academy of Dermatology, 1989

1. J Am Acad Dermatol. 1989 Jun;20(6):1123-4. Complete resolution of Kaposi's sarcoma with s... more 1. J Am Acad Dermatol. 1989 Jun;20(6):1123-4. Complete resolution of Kaposi's sarcoma with systemic etretinate therapy in a patient with mycosis fungoides. Sharpe RJ, Kadin ME, Harmon DC, Imber MJ, Anderson RR. PMID: 2754062 [PubMed - indexed for MEDLINE]. ...

Research paper thumbnail of Extracorporeal photopheresis in psoriasis vulgaris: Clinical and immunologic observations

Journal of the American Academy of Dermatology, 1990

10. Brown MD, EllisC,Voorhees JJ. Cyclosporinc A: a review of its dermatologic applications. Semi... more 10. Brown MD, EllisC,Voorhees JJ. Cyclosporinc A: a review of its dermatologic applications. Semin Dermatol 1987; 6:2-9. II. Cunliffe WJ. Pemphigus Ioliaceus and response to cyclosporin. Br J Dermatol 1987; 117(suppl 32): 114-6. 12. Palestine AG, Nussemblatt RB, Chance. Sideeffects of systemic cyclosporin in patients not undergoing transplantation. Am J Med 1984;77:652-6. 13. Boungneres PF, Cavel lC, Castano L, et al. Factors associated with early remission of type I diabetes in children Cyclosporine therapy in pemphigus ' . u!,:;arjs treated with cyclosporine. N Engl

Research paper thumbnail of Cutaneous manifestations of postthymic T cell malignancies: Description of five clinicopathologic subtypes

Journal of the American Academy of Dermatology, 1990

This study was undertaken to identify the cutaneous manifestations among different prognostic sub... more This study was undertaken to identify the cutaneous manifestations among different prognostic subgroups of postthymic T cell malignancies. Cutaneous involvement was demonstrated in 43 of 88 cases. We recognized five clinicopathologic subtypes: typeI, classical cu-taneousT celllymphoma (Cf'Cl.) or mycosis fungoides, sixcases; type II, primary large cell type CTCL, Ki-l antigen (Ki-l + or Ki-T"), seven cases; typeIII, primary angioinvasive T celllymphoma, three cases; type IV,human T-Iymphotropic virus typeI (HTLV-I+) adult T cellleukemia/lymphoma (ATL), eightcases; type V,secondary cutaneous involvement by peripheralT celllymphoma (PTL), 19cases. Primary CTCL and ATL tendto involve papillary dermiswith or withoutepidermotropism, whereas PTL and angioinvasive T cell lymphoma predominantly affectskin adnexae, vessels, and subcutis. Cutaneous lesions in type V PTL are heterogeneous and may be confused with panniculitis, vasculitis, or an eczematous eruption. ClassicCTCL, Ki-l + lymphoma, and angioinvasive T cell lymphoma have a chroniccourse, whereas ATL, Ki-Ir large celllymphoma, andP1Lareclinically aggressive.

Research paper thumbnail of ULTRASTRUCTURAL LOCALIZATION OF IMMUNOGLOBULINS IN BULLOUS PEMPHIGOID SKIN. Employment of a New Peroxidase - Antiperoxidase Multistep Method

Journal of Investigative Dermatology, 1975

Abstract A multistep immunochemical method was developed for light and electron microscopy utiliz... more Abstract A multistep immunochemical method was developed for light and electron microscopy utilizing horseradish peroxidase as an immunologically bound marker. The method was applied to studies of in vivo bound IgG in bullous pemphigoid skin and of ...

Research paper thumbnail of Molecular evidence for a clonal relationship between lymphomatoid papulosis and Ki-1 positive large cell anaplastic lymphoma

Journal of Dermatological Science, 1993

Currently, considerable controversy surrounds questions about the clonal evolution of lymphomas i... more Currently, considerable controversy surrounds questions about the clonal evolution of lymphomas in patients with lymphomatoid papulosis. In order to analyze a possible clonal relationship between lesions of lymphomatoid papulosis and a Ki l+ large cell anaplastic lymphoma in the same patient, a highly specific molecular probe for the malignant lymphoid clone of the large cell anaplastic lymphoma was developed. As a clone specific molecular marker, highly variable junctional sequences of rearranged T-cell receptor-gamma genes were used. An oligonucleotide primer complementary to these sequences was synthesized and, using the polymerase chain reaction, clone specific DNA was detected in all lesions of lymphomatoid papulosis of the patient. These results provide evidence for a clonal relationship between lesions of lymphomatoid papulosis and large cell anaplastic lymphoma developing in the same patient.