M. Naeemullah - Academia.edu (original) (raw)

Papers by M. Naeemullah

Research paper thumbnail of Screening of mint germplasm under field and glasshouse conditions

pu.edu.pk

... Atif Jamal, Muhammad Naeemullah, M. Shahid Masood, Muhammad Zakria, Riffat Tahira, Umer Iqbal... more ... Atif Jamal, Muhammad Naeemullah, M. Shahid Masood, Muhammad Zakria, Riffat Tahira, Umer Iqbal and SM Iqbal. ... menthae), powdery mildew (Erysiphe cischoracearum), leaf spot (Alternaria alternate), aerial blight (Rhizoctonia solani), wilt (Verticillium dahlia), stem rot ...

Research paper thumbnail of PLANT PARASITIC NEMATODES ASSOCIATED WITH DIFFERENT MENTHA SPECIES

Pak. J. Nematol, 2012

Thirteen mint species were evaluated for the presence of nematode fauna associated with their rhi... more Thirteen mint species were evaluated for the presence of nematode fauna associated with their rhizospheres. Six plant parasitic and six saprophytic nematode genera were found associated with mint rhizosphere. Tylenchorhynchus spp., was found to be ...

Research paper thumbnail of Genetic divergence in Amaranthus collected from Pakistan

In the present study different species (Amaranthus hypochondriacus & A. tricolor) of amaranthus w... more In the present study different species (Amaranthus hypochondriacus & A. tricolor) of amaranthus were collected from different agro-ecological zones of Pakistan and compared for their phenotypic and nutritional traits. A UPGMA cluster, grouped the 13 amaranthus genotypes into two major clusters, I and II, differentiating the ornamental amaranthus cultivars from edible. However, comparative view of the cluster showed that the Amaranthus hypochondriacus were closest to the China variety than to the Amaranthus tricolor according to their morphological characters. Optimal level of carbohydrates, fats, proteins and moisture contents were observed in 7033 (192.7 mg/ml), 7051(30.68 %) and 7033 (102.7 µg/ml) and 7034 (16 %) respectively, of Gonar, Northern Area and AJK of Pakistan. Accession numbers were allotted to the amaranthus germplasm and preservation of there seeds in the gene bank at PGRI, Islamabad, for future research activities including evaluation, characterization and crop improvement.

Research paper thumbnail of Genetic variation in the living repository of Ocimum germplasm

Pakistan Journal of …, 2011

... Shazia Erum*, Muhammad Naeemullah, Shahid Masood** and Muhammad Irfan Khan* ... polyploidy, c... more ... Shazia Erum*, Muhammad Naeemullah, Shahid Masood** and Muhammad Irfan Khan* ... polyploidy, common occurrences within Ocimum genus and have created taxonomic confusion making it difficult to understand the genetic rela-tionship between many basils (Harley et al ...

Research paper thumbnail of Characterization of brown planthopper strains based on their differential responses to introgressed resistance genes and on mitochondrial DNA polymorphism

Applied Entomology and Zoology, 2009

Research paper thumbnail of Molecular cloning, transcriptional regulation, and differential expression profiling of vitellogenin in two wing-morphs of the brown planthopper, Nilaparvata lugens Stal (Hemiptera: Delphacidae)

The brown planthopper, Nilaparvata lugens, is a serious pest of rice crops throughout Asia and ex... more The brown planthopper, Nilaparvata lugens, is a serious pest of rice crops throughout Asia and exhibits wing dimorphism, with brachypterous adults having reduced wings and macropterous adults possessing fully developed wings. To understand the reproductive strategies in two wing-morphs of this insect, the transcript encoding the major yolk protein precursor, vitellogenin (Vg), was cloned. The complete mRNA transcript was 6314 bp, which encodes a protein of 2063 residues including an 18-residue putative signal peptide. Analysis of the mature protein revealed two vitellogenin-N (or lipoprotein aminoterminal) domains near the N-terminus and a von Willebrand factor type D domain near the C-terminus. In addition, a highly conserved motif GL/ICG, and a number of cysteine residues were identified near the C-terminus. Northern blot analysis identified a~6.8 kb Vg gene transcript that was expressed exclusively in the adult female fat body cells. The expression profile revealed that the Vg gene starts to be expressed earlier (on day 3) in brachypters as compared to macropters where the mRNA transcript was observed on day 4. However, in both morphs, the amount of Vg mRNA increased to reach high levels during vitellogenic periods [from day 4 (in brachypters) and day 5 (in macropters) and onwards]. Reflecting the RNA transcription pattern, the Vg signal was detected by immunoblotting on day 3 and day 4 in haemolymph of brachypterous and macropterous females, respectively, and that was increased every day and remained high during the vitellogenic periods. Furthermore, the topical application of juvenile hormone (JH) III had up-regulated the Vg gene expression suggesting that the Vg gene is regulated by JH in N. lugens. In addition, it was demonstrated by Southern blot analysis that there exists a single copy of the gene in the N. lugens genome. A delayed trend in expression (of both the transcript and the protein) demonstrated by macropterous females in the present studies supports the hypothesis of prereproductive long distance migration in this wing-dimorphic species.

Research paper thumbnail of Non-specific resistance in brown planthopper resistant indica rice varieties against Plodia interpunctella (Lepidoptera: Phycitidae)

Entomological Science, 2004

... A decreased infestation of the maize weevil, Sitophilus zeamais Mutchulsky, in stored grain w... more ... A decreased infestation of the maize weevil, Sitophilus zeamais Mutchulsky, in stored grain was attributed to kernel hardiness (Eden 1952), which ... as introgression lines, in which BPH resistance genes are integrated into the rice genome from resistance sources (Sharma et al. ...

Research paper thumbnail of Screening of mint germplasm under field and glasshouse conditions

pu.edu.pk

... Atif Jamal, Muhammad Naeemullah, M. Shahid Masood, Muhammad Zakria, Riffat Tahira, Umer Iqbal... more ... Atif Jamal, Muhammad Naeemullah, M. Shahid Masood, Muhammad Zakria, Riffat Tahira, Umer Iqbal and SM Iqbal. ... menthae), powdery mildew (Erysiphe cischoracearum), leaf spot (Alternaria alternate), aerial blight (Rhizoctonia solani), wilt (Verticillium dahlia), stem rot ...

Research paper thumbnail of PLANT PARASITIC NEMATODES ASSOCIATED WITH DIFFERENT MENTHA SPECIES

Pak. J. Nematol, 2012

Thirteen mint species were evaluated for the presence of nematode fauna associated with their rhi... more Thirteen mint species were evaluated for the presence of nematode fauna associated with their rhizospheres. Six plant parasitic and six saprophytic nematode genera were found associated with mint rhizosphere. Tylenchorhynchus spp., was found to be ...

Research paper thumbnail of Genetic divergence in Amaranthus collected from Pakistan

In the present study different species (Amaranthus hypochondriacus & A. tricolor) of amaranthus w... more In the present study different species (Amaranthus hypochondriacus & A. tricolor) of amaranthus were collected from different agro-ecological zones of Pakistan and compared for their phenotypic and nutritional traits. A UPGMA cluster, grouped the 13 amaranthus genotypes into two major clusters, I and II, differentiating the ornamental amaranthus cultivars from edible. However, comparative view of the cluster showed that the Amaranthus hypochondriacus were closest to the China variety than to the Amaranthus tricolor according to their morphological characters. Optimal level of carbohydrates, fats, proteins and moisture contents were observed in 7033 (192.7 mg/ml), 7051(30.68 %) and 7033 (102.7 µg/ml) and 7034 (16 %) respectively, of Gonar, Northern Area and AJK of Pakistan. Accession numbers were allotted to the amaranthus germplasm and preservation of there seeds in the gene bank at PGRI, Islamabad, for future research activities including evaluation, characterization and crop improvement.

Research paper thumbnail of Genetic variation in the living repository of Ocimum germplasm

Pakistan Journal of …, 2011

... Shazia Erum*, Muhammad Naeemullah, Shahid Masood** and Muhammad Irfan Khan* ... polyploidy, c... more ... Shazia Erum*, Muhammad Naeemullah, Shahid Masood** and Muhammad Irfan Khan* ... polyploidy, common occurrences within Ocimum genus and have created taxonomic confusion making it difficult to understand the genetic rela-tionship between many basils (Harley et al ...

Research paper thumbnail of Characterization of brown planthopper strains based on their differential responses to introgressed resistance genes and on mitochondrial DNA polymorphism

Applied Entomology and Zoology, 2009

Research paper thumbnail of Molecular cloning, transcriptional regulation, and differential expression profiling of vitellogenin in two wing-morphs of the brown planthopper, Nilaparvata lugens Stal (Hemiptera: Delphacidae)

The brown planthopper, Nilaparvata lugens, is a serious pest of rice crops throughout Asia and ex... more The brown planthopper, Nilaparvata lugens, is a serious pest of rice crops throughout Asia and exhibits wing dimorphism, with brachypterous adults having reduced wings and macropterous adults possessing fully developed wings. To understand the reproductive strategies in two wing-morphs of this insect, the transcript encoding the major yolk protein precursor, vitellogenin (Vg), was cloned. The complete mRNA transcript was 6314 bp, which encodes a protein of 2063 residues including an 18-residue putative signal peptide. Analysis of the mature protein revealed two vitellogenin-N (or lipoprotein aminoterminal) domains near the N-terminus and a von Willebrand factor type D domain near the C-terminus. In addition, a highly conserved motif GL/ICG, and a number of cysteine residues were identified near the C-terminus. Northern blot analysis identified a~6.8 kb Vg gene transcript that was expressed exclusively in the adult female fat body cells. The expression profile revealed that the Vg gene starts to be expressed earlier (on day 3) in brachypters as compared to macropters where the mRNA transcript was observed on day 4. However, in both morphs, the amount of Vg mRNA increased to reach high levels during vitellogenic periods [from day 4 (in brachypters) and day 5 (in macropters) and onwards]. Reflecting the RNA transcription pattern, the Vg signal was detected by immunoblotting on day 3 and day 4 in haemolymph of brachypterous and macropterous females, respectively, and that was increased every day and remained high during the vitellogenic periods. Furthermore, the topical application of juvenile hormone (JH) III had up-regulated the Vg gene expression suggesting that the Vg gene is regulated by JH in N. lugens. In addition, it was demonstrated by Southern blot analysis that there exists a single copy of the gene in the N. lugens genome. A delayed trend in expression (of both the transcript and the protein) demonstrated by macropterous females in the present studies supports the hypothesis of prereproductive long distance migration in this wing-dimorphic species.

Research paper thumbnail of Non-specific resistance in brown planthopper resistant indica rice varieties against Plodia interpunctella (Lepidoptera: Phycitidae)

Entomological Science, 2004

... A decreased infestation of the maize weevil, Sitophilus zeamais Mutchulsky, in stored grain w... more ... A decreased infestation of the maize weevil, Sitophilus zeamais Mutchulsky, in stored grain was attributed to kernel hardiness (Eden 1952), which ... as introgression lines, in which BPH resistance genes are integrated into the rice genome from resistance sources (Sharma et al. ...