Mannana Ntoampe - Academia.edu (original) (raw)
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Egypt-Japan University of Science and Technology (E-JUST)
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Papers by Mannana Ntoampe
WIT Transactions on Ecology and the Environment, 2010
I am also thankful to my grandmother, Annah Ntoampe and my aunt, Palesa Ntoampe for encouraging m... more I am also thankful to my grandmother, Annah Ntoampe and my aunt, Palesa Ntoampe for encouraging me through it all, as well as Mr M. Mabusela and Mr. B. Mothibedi for their technical support. Above all, I would like to thank my God who makes all things possible. The Lord who renewed my strength even when all I wanted to do was to give up and throw in the towel. vi
Many strains of Gram-negative bacteria, such as Pseudomonas, are able to utilize a variety of unu... more Many strains of Gram-negative bacteria, such as Pseudomonas, are able to utilize a variety of unusual chemicals, including a wide range or aromatic hydrocarbons and their derivatives for growth. Bacteria with the potential to degrade sodium benzoate were isolated, identified and grown as biofilms on sodium benzoate in a laboratory-scale fluidized bed biofilm bioreactor. Four Pseudomonas strains identified as P. aeruginosa (BDS2) P. putida (BDS1 and GR1) and Burkholderia cepecia (GR3FAR) were used in a laboratory-scale FBBR together with two Bacillus strains-Bacillus macroides (SBSY4) and Bacillus simplex (MAR). Sodium benzoate biodegradation capacities of these species were compared under batch and continuous operations. Biofilm and planktonic bacterial growth dynamics were monitored by plate counts, and optical density measurements (230nm) determined benzoate biodegradation. Overall, higher attached and planktonic bacterial counts were determined under batch compared to continuous mode. In addition to this, the ability of attached cells to use sodium benzoate as their sole carbon source was compared to their suspended counterparts in a batch system. There were more attached counts compared to suspended cells and attached cells apparently degraded sodium benzoate better than planktonic cells. Similarly, higher rates of benzoate depletion were found to occur under batch compared to the continuous system. It thus appeared that more cell growth implied more substrate consumption. SEM showed attached cells and microcolonies of all the isolates on GAC, indicating their biofilmforming abilities.
Bioresource Technology, 2008
WIT Transactions on Ecology and the Environment, 2010
I am also thankful to my grandmother, Annah Ntoampe and my aunt, Palesa Ntoampe for encouraging m... more I am also thankful to my grandmother, Annah Ntoampe and my aunt, Palesa Ntoampe for encouraging me through it all, as well as Mr M. Mabusela and Mr. B. Mothibedi for their technical support. Above all, I would like to thank my God who makes all things possible. The Lord who renewed my strength even when all I wanted to do was to give up and throw in the towel. vi
Many strains of Gram-negative bacteria, such as Pseudomonas, are able to utilize a variety of unu... more Many strains of Gram-negative bacteria, such as Pseudomonas, are able to utilize a variety of unusual chemicals, including a wide range or aromatic hydrocarbons and their derivatives for growth. Bacteria with the potential to degrade sodium benzoate were isolated, identified and grown as biofilms on sodium benzoate in a laboratory-scale fluidized bed biofilm bioreactor. Four Pseudomonas strains identified as P. aeruginosa (BDS2) P. putida (BDS1 and GR1) and Burkholderia cepecia (GR3FAR) were used in a laboratory-scale FBBR together with two Bacillus strains-Bacillus macroides (SBSY4) and Bacillus simplex (MAR). Sodium benzoate biodegradation capacities of these species were compared under batch and continuous operations. Biofilm and planktonic bacterial growth dynamics were monitored by plate counts, and optical density measurements (230nm) determined benzoate biodegradation. Overall, higher attached and planktonic bacterial counts were determined under batch compared to continuous mode. In addition to this, the ability of attached cells to use sodium benzoate as their sole carbon source was compared to their suspended counterparts in a batch system. There were more attached counts compared to suspended cells and attached cells apparently degraded sodium benzoate better than planktonic cells. Similarly, higher rates of benzoate depletion were found to occur under batch compared to the continuous system. It thus appeared that more cell growth implied more substrate consumption. SEM showed attached cells and microcolonies of all the isolates on GAC, indicating their biofilmforming abilities.
Bioresource Technology, 2008