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Research paper thumbnail of In vitro Differentiation of Mouse Teratocarcinoma Cells Monitored by Intermediate Filament Expression

Differentiation, 1982

Teratocarcinoma differentiation has been studied using sera specific for each of the five interme... more Teratocarcinoma differentiation has been studied using sera specific for each of the five intermediate filament (IF) classes. These antibodies distinguish cells of epithelial, muscle, neural, astrocytic, and mesenchymal origin. In embryoid bodies, derived from embryo transplants and obtained in the ascitic fluid by transplantation of teratocarcinoma, the cells of the inner cellular mass did not express any of these intermediate filament types while the outer cells expressed cytokeratin. Intermediate filament expression in the embryoid body thus appears analogous to that in the blastocyst and differs from that in embryonal carcinoma (EC) lines. Twelve EC lines have now been shown to express vimentin although in some EC lines not all cells express vimentin. Other established permanent differentiated cell lines, derived from EC lines in vitro or from tumors in vivo, have been characterized with respect to the type of IF they contain. The distribution of different IF types has been examined in EC cells induced to differentiate by addition of retinoic acid. The proportion of cells expressing each type of intermediate filament appears to depend on the EC cell line used, on the inducing agent, and on the length of treatment. Thus, for instance, F9 cells express cytokeratin, PCC3 derivatives express vimentin, many 1009 derivatives express either glial fibrillar acidic protein (GFA) or neurofilament proteins. Overall the results obtained are in excellent agreement with emerging principles of intermediate filament expression during embryonic differentiation, thus emphasizing the potential use of the various EC lines to study differentiation in culture.

Research paper thumbnail of Actin and tubulin in teratocarcinoma cells

Developmental Biology, 1978

Research paper thumbnail of Amelioration of epidermolysis bullosa by transfer of wild-type bone marrow cells

Blood, 2008

The recessive dystrophic form of epidermolysis bullosa (RDEB) is a disorder of incurable skin fra... more The recessive dystrophic form of epidermolysis bullosa (RDEB) is a disorder of incurable skin fragility and blistering caused by mutations in the type VII collagen gene (Col7a1). The absence of type VII collagen production leads to the loss of adhesion at the basement membrane zone due to the absence of anchoring fibrils, which are composed of type VII collagen. We report that wild-type, congenic bone marrow cells homed to damaged skin, produced type VII collagen protein and anchoring fibrils, ameliorated skin fragility, and reduced lethality in the murine model of RDEB generated by targeted Col7a1 disruption. These data provide the first evidence that a population of marrow cells can correct the basement membrane zone defect found in mice with RDEB and offer a potentially valuable approach for treatment of human RDEB and other extracellular matrix disorders.

Research paper thumbnail of Sarcoma Derived from Cultured Mesenchymal Stem Cells

Stem Cells, 2007

STEM CELLS® is a monthly publication, it has been published continuously since 1983. The genetics... more STEM CELLS® is a monthly publication, it has been published continuously since 1983. The genetics and genomics; translational and clinical research; technology development. embryonic stem cells; tissue-specific stem cells; cancer stem cells; the stem cell niche; stem cell STEM CELLS®, an international peer-reviewed journal, covers all aspects of stem cell research:

Research paper thumbnail of In vitro Differentiation of Mouse Teratocarcinoma Cells Monitored by Intermediate Filament Expression

Differentiation, 1982

Teratocarcinoma differentiation has been studied using sera specific for each of the five interme... more Teratocarcinoma differentiation has been studied using sera specific for each of the five intermediate filament (IF) classes. These antibodies distinguish cells of epithelial, muscle, neural, astrocytic, and mesenchymal origin. In embryoid bodies, derived from embryo transplants and obtained in the ascitic fluid by transplantation of teratocarcinoma, the cells of the inner cellular mass did not express any of these intermediate filament types while the outer cells expressed cytokeratin. Intermediate filament expression in the embryoid body thus appears analogous to that in the blastocyst and differs from that in embryonal carcinoma (EC) lines. Twelve EC lines have now been shown to express vimentin although in some EC lines not all cells express vimentin. Other established permanent differentiated cell lines, derived from EC lines in vitro or from tumors in vivo, have been characterized with respect to the type of IF they contain. The distribution of different IF types has been examined in EC cells induced to differentiate by addition of retinoic acid. The proportion of cells expressing each type of intermediate filament appears to depend on the EC cell line used, on the inducing agent, and on the length of treatment. Thus, for instance, F9 cells express cytokeratin, PCC3 derivatives express vimentin, many 1009 derivatives express either glial fibrillar acidic protein (GFA) or neurofilament proteins. Overall the results obtained are in excellent agreement with emerging principles of intermediate filament expression during embryonic differentiation, thus emphasizing the potential use of the various EC lines to study differentiation in culture.

Research paper thumbnail of Actin and tubulin in teratocarcinoma cells

Developmental Biology, 1978

Research paper thumbnail of Amelioration of epidermolysis bullosa by transfer of wild-type bone marrow cells

Blood, 2008

The recessive dystrophic form of epidermolysis bullosa (RDEB) is a disorder of incurable skin fra... more The recessive dystrophic form of epidermolysis bullosa (RDEB) is a disorder of incurable skin fragility and blistering caused by mutations in the type VII collagen gene (Col7a1). The absence of type VII collagen production leads to the loss of adhesion at the basement membrane zone due to the absence of anchoring fibrils, which are composed of type VII collagen. We report that wild-type, congenic bone marrow cells homed to damaged skin, produced type VII collagen protein and anchoring fibrils, ameliorated skin fragility, and reduced lethality in the murine model of RDEB generated by targeted Col7a1 disruption. These data provide the first evidence that a population of marrow cells can correct the basement membrane zone defect found in mice with RDEB and offer a potentially valuable approach for treatment of human RDEB and other extracellular matrix disorders.

Research paper thumbnail of Sarcoma Derived from Cultured Mesenchymal Stem Cells

Stem Cells, 2007

STEM CELLS® is a monthly publication, it has been published continuously since 1983. The genetics... more STEM CELLS® is a monthly publication, it has been published continuously since 1983. The genetics and genomics; translational and clinical research; technology development. embryonic stem cells; tissue-specific stem cells; cancer stem cells; the stem cell niche; stem cell STEM CELLS®, an international peer-reviewed journal, covers all aspects of stem cell research:

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