M. Serek - Academia.edu (original) (raw)

Papers by M. Serek

Research paper thumbnail of Use of genetic manipulation to reduce ethylene sensitivity in ornamental plants

Research paper thumbnail of Expression of the etr1-1 allele under control of floral- and senescence-specific promoters in Pelargonium zonale

Research paper thumbnail of The establishment of effective regeneration system and in vitro culture of Campanula

Research paper thumbnail of Development of cleaved amplified polymorphic sequence (CAPS) markers that may indicate ethylene sensitivity in miniature potted roses

Research paper thumbnail of Anti-ethylene Treatments for Potted Christmas Cactus-Efficacy of Inhibitors of Ethylene Action and Biosynthesis

HortScience

Spraying flowering plants of Schlumbergera truncata (Haw.) `White Christmas' with aminoethoxy... more Spraying flowering plants of Schlumbergera truncata (Haw.) `White Christmas' with aminoethoxyvinylglycine (AVG), an inhibitor of ethylene biosynthesis, did not prevent the rapid loss of flower buds caused by exposure to 1 μl of ethylene/liter. Treatment with the silver thiosulfate anionic complex (STS) strongly inhibited such effects. The rate of bud drop in ethylene-free air (interior environment room) was somewhat reduced by AVG treatment, although total display life of treated plants was not significantly different from that of the controls. STS treatment reduced the rate of bud drop, and increased display life by 20 %.

Research paper thumbnail of Protoplast Culture in Petunia Hybrida and Effects of Cell Cycle Inhibitors on Cell Division Frequency

Acta Horticulturae

Protoplasts were isolated from six different Petunia hybrida genotypes and cultured embedded in a... more Protoplasts were isolated from six different Petunia hybrida genotypes and cultured embedded in alginate in liquid medium 8pm7 (1.0 mg L -1 benzylaminopurin (BAP) + 1.0 mg L -1 indole acetic acid (IAA) + 2.5% sucrose). This medium was replaced after seven to nine days by callus induction medium KF1 (0.5 mg L -1 BAP + 0.5 mg L -1 IAA and 1.8% sucrose) and later by KF2 (0.5 mg L -1 BAP + 0.5 mg L -1 IAA and 1.3% sucrose). Thereafter, calluses were transferred to solidified PET 2 medium (1 mg L -1 BAP + 2 mg L -1 IAA). The protoplast yield varied between 0.5 and 5.6 * 10 6 protoplasts per gram fresh weight. Cell division frequencies between 15 and 55% were observed. Shoot regeneration was obtained from three genotypes. In terms of a later fusion and selection of the heterologous hybrids the effects of the cell cycle inhibitors Rhodamine 6G (R6G) and lodoacetamide (IOA) were analysed. The treatment with different concentrations of R6G and IOA reduced cell division markedly, but pronounced differences between experiments did not allow the identification of a appropriate concentration yet.

Research paper thumbnail of Effects of cytokinin treatment of nodal cuts and petioles on shoot regeneration of Campanula portenschlagiana ‘Blue Ocean’

Research paper thumbnail of Expression of genes for ethylene biosynthetic enzymes and anethylene receptor in senescing flowers of miniature potted roses

The Journal of Horticultural Science and Biotechnology

Summary Expression of genes for the ethylene biosynthetic enzymes ACC oxidase and ACC synthase, a... more Summary Expression of genes for the ethylene biosynthetic enzymes ACC oxidase and ACC synthase, and the gene for a putative ethylene receptor, termed RhETR, in miniature roses (Rosa hybrida) were examined during flower senescence of the long-lasting cv. Vanilla and cv. Bronze, which has a short flower life. The abundance of ACC oxidase transcript increased during the late stages of flower development in petals of both cultivars, being higher for `Bronze' than for `Vanilla'. The ACC synthase transcript increased during flower senescence in `Vanilla' but remained constant at a low level in `Bronze'. The expression of RhETR was distinctly higher in the cultivar with short flower life (`Bronze') than in the long-lasting cultivar (`Vanilla'), and modulation of receptor levels was also observed during flower development. Exposure to low ethylene concentrations resulted in an up-regulation of RhETR in flowers of both cultivars. Differences in the expression of the putative ethylene receptor of cultivars with short or long flower life suggest that variation in flower longevity may be due to differences in receptor levels during flower development.

Research paper thumbnail of Response of F1 breeding lines of Rosa hybrida to exogenous ethylene treatment evaluated at physiological and molecular levels

Research paper thumbnail of Induction of Ethylene Insensitivity Into Oncidium and Odontoglossum Orchid Species for Improvement of Display Life

Research paper thumbnail of Novel gaseous ethylene inhibitor prevents ethylene effects in potted flowering plants

Research paper thumbnail of Plant Regeneration from Different Explant Types of Streptocarpus Spec. In Vitro

Acta Horticulturae, 2006

In order to develop an in vitro regeneration protocol for different Streptocarpus species, we inv... more In order to develop an in vitro regeneration protocol for different Streptocarpus species, we investigated the effects of three factors: surface sterilization, regeneration medium and explant type. For surface sterilization the influence of an ethanol (70%) treatment for 30 s was tested, before the explants were immersed in 1.5% sodium hypochlorite for 10 min. Our experiments showed no significant difference regarding contamination of explants between the surface sterilization with and without ethanol. On the other hand, the treatment with 70% ethanol led to damage of the leaf explants regarding regeneration, especially the number of shootlets. The variation of indole acetic acid (IAA) and benzylaminopurine (BAP) concentrations in the regeneration medium revealed, that a combination of 1.0 mg L IAA and 1.0 mg L -1 BAP was most efficient for shoot regeneration in three species (S1, S3, S4). However, the best quality and regeneration response of the fourth species (S7) was obtained with 1.0 mg L -1 IAA and 0.1 mg L -1 BAP. The differences of the most suitable combination of phytohormones showed, that it was not possible to designate a uniform optimal medium for all of Streptocarpus species. In addition, different explant types were compared. Taking into consideration the regeneration intensity, the expenditure of time for explant preparation and material costs, we concluded that the most suitable explant type were leaf blade explants.

Research paper thumbnail of Efficiency of Pmi/Mannose as a Selectable Marker for Transformation of Kalanchoe

Acta Horticulturae, 2012

ABSTRACT Mannose is an unsuitable carbon source for many plants. Various factors were studied to ... more ABSTRACT Mannose is an unsuitable carbon source for many plants. Various factors were studied to evaluate the efficacy of mannose in the for production of transgenic plants using Agrobacterium tumefaciens-mediated transformation of leaf explants of Kalanchoe blossfeldiana. To establish a non-antibiotic selection system that utilizes the phosphomannose-isomerase (PMI) gene from E. coli, we determined the optimum mannose concentration for selecting transformed cells. Shoot organogenesis in K. blossfeldiana leaf explants was suppressed effectively on medium supplemented with mannose, or mannose with glucose, which indicated that Kalanchoe cells had little or no PMI activity and could not utilize mannose as a carbon source. The optimum concentration of mannose was found to eliminate non-transgenic shoots. Transformed plants were confirmed by GUS assay. Transformation ranged up to ca. 40%. The PMI/mannose system provides an efficient method for selecting without antibiotics or herbicides.

Research paper thumbnail of Plant Regeneration via Somatic Embryogenesis in Schlumbergera Truncate

Research paper thumbnail of Enhanced Regenerative Capacity in Rhipsalidopsis and Schlumbergera Explants by in Vitro Manipulations

Acta Horticulturae, 2006

Several plant species are recalcitrant when considering regeneration through adventitious organs.... more Several plant species are recalcitrant when considering regeneration through adventitious organs. Establishment of regeneration systems for the plants of interest is a pre-requisite for further work such as genetic transformation and induced mutation. The cactus plants belonging to species Schlumbergera and Rhipsalidopsis were found to be recalcitrant. In addition to this problem, they were very slow to grow. However, it was possible to achieve high regenerative ability in explants of in vitro cultures of these cacti. The initial phylloclade explants established from adult plants grown in greenhouse were found to be recalcitrant. These explants produced only axillary shoots from the pre-formed shoot primodia when treated with exogenous plant hormones, but they failed to produce any adventitious shoots. There was a significant improvement in adventitious shoot formation in phylloclade explants, more particularly, of Rhipsalidopsis in long-term cultures. After the first subculture, sporadic adventitious shoot formation began, and after the third subculture 87% explants of one of the cultiyars of Rhipsalidopsis produced adventitious shoots. There was an increase in the regenerative capacity in the explants of Schlumbergera, however, the efficiency was not to the same extent as for the other species. Callus cultures were found to be more regenerative compared to the phylloclade explants. The results clearly indicated that the in vitro manipulations helped in increasing the capacity to regenerate.

Research paper thumbnail of Effects and Interactions of Ethylene and Abscisic Acid in Detached and Intact Miniature Rose Flowers

IV International Conference on Postharvest Science, 2001

Research paper thumbnail of Physiological and Genetic Reasons for Different Postharvest Characteristics in Miniature Potted Roses

VII International Symposium on Postharvest Physiology of Ornamental Plants, 2001

Research paper thumbnail of Zakizadeh, H., Lütken H., Sriskandarajah S., M. Serek and R. Müller (2012) Transformation of miniature potted rose (Rosa hybrida cv. Linda) with PSAG12-ipt gene enhances resistance to leaf senescence and exogenous ethylene. Plant Cell Reports (Online, Dec 2012 :DOI 10.1007/s00299-012-1354-5)

Research paper thumbnail of Chemical Compounds Interacting with the Ethylene Receptor in Ornamental Crops

X International Symposium on Postharvest Quality of Ornamental Plants, 2015

Research paper thumbnail of Molecular Genetic Investigation of Malformed Flower Types of Rosa Hybrid L

XXIV International Eucarpia Symposium Section Ornamentals: Ornamental Breeding Worldwide, 2012

Research paper thumbnail of Use of genetic manipulation to reduce ethylene sensitivity in ornamental plants

Research paper thumbnail of Expression of the etr1-1 allele under control of floral- and senescence-specific promoters in Pelargonium zonale

Research paper thumbnail of The establishment of effective regeneration system and in vitro culture of Campanula

Research paper thumbnail of Development of cleaved amplified polymorphic sequence (CAPS) markers that may indicate ethylene sensitivity in miniature potted roses

Research paper thumbnail of Anti-ethylene Treatments for Potted Christmas Cactus-Efficacy of Inhibitors of Ethylene Action and Biosynthesis

HortScience

Spraying flowering plants of Schlumbergera truncata (Haw.) `White Christmas' with aminoethoxy... more Spraying flowering plants of Schlumbergera truncata (Haw.) `White Christmas' with aminoethoxyvinylglycine (AVG), an inhibitor of ethylene biosynthesis, did not prevent the rapid loss of flower buds caused by exposure to 1 μl of ethylene/liter. Treatment with the silver thiosulfate anionic complex (STS) strongly inhibited such effects. The rate of bud drop in ethylene-free air (interior environment room) was somewhat reduced by AVG treatment, although total display life of treated plants was not significantly different from that of the controls. STS treatment reduced the rate of bud drop, and increased display life by 20 %.

Research paper thumbnail of Protoplast Culture in Petunia Hybrida and Effects of Cell Cycle Inhibitors on Cell Division Frequency

Acta Horticulturae

Protoplasts were isolated from six different Petunia hybrida genotypes and cultured embedded in a... more Protoplasts were isolated from six different Petunia hybrida genotypes and cultured embedded in alginate in liquid medium 8pm7 (1.0 mg L -1 benzylaminopurin (BAP) + 1.0 mg L -1 indole acetic acid (IAA) + 2.5% sucrose). This medium was replaced after seven to nine days by callus induction medium KF1 (0.5 mg L -1 BAP + 0.5 mg L -1 IAA and 1.8% sucrose) and later by KF2 (0.5 mg L -1 BAP + 0.5 mg L -1 IAA and 1.3% sucrose). Thereafter, calluses were transferred to solidified PET 2 medium (1 mg L -1 BAP + 2 mg L -1 IAA). The protoplast yield varied between 0.5 and 5.6 * 10 6 protoplasts per gram fresh weight. Cell division frequencies between 15 and 55% were observed. Shoot regeneration was obtained from three genotypes. In terms of a later fusion and selection of the heterologous hybrids the effects of the cell cycle inhibitors Rhodamine 6G (R6G) and lodoacetamide (IOA) were analysed. The treatment with different concentrations of R6G and IOA reduced cell division markedly, but pronounced differences between experiments did not allow the identification of a appropriate concentration yet.

Research paper thumbnail of Effects of cytokinin treatment of nodal cuts and petioles on shoot regeneration of Campanula portenschlagiana ‘Blue Ocean’

Research paper thumbnail of Expression of genes for ethylene biosynthetic enzymes and anethylene receptor in senescing flowers of miniature potted roses

The Journal of Horticultural Science and Biotechnology

Summary Expression of genes for the ethylene biosynthetic enzymes ACC oxidase and ACC synthase, a... more Summary Expression of genes for the ethylene biosynthetic enzymes ACC oxidase and ACC synthase, and the gene for a putative ethylene receptor, termed RhETR, in miniature roses (Rosa hybrida) were examined during flower senescence of the long-lasting cv. Vanilla and cv. Bronze, which has a short flower life. The abundance of ACC oxidase transcript increased during the late stages of flower development in petals of both cultivars, being higher for `Bronze' than for `Vanilla'. The ACC synthase transcript increased during flower senescence in `Vanilla' but remained constant at a low level in `Bronze'. The expression of RhETR was distinctly higher in the cultivar with short flower life (`Bronze') than in the long-lasting cultivar (`Vanilla'), and modulation of receptor levels was also observed during flower development. Exposure to low ethylene concentrations resulted in an up-regulation of RhETR in flowers of both cultivars. Differences in the expression of the putative ethylene receptor of cultivars with short or long flower life suggest that variation in flower longevity may be due to differences in receptor levels during flower development.

Research paper thumbnail of Response of F1 breeding lines of Rosa hybrida to exogenous ethylene treatment evaluated at physiological and molecular levels

Research paper thumbnail of Induction of Ethylene Insensitivity Into Oncidium and Odontoglossum Orchid Species for Improvement of Display Life

Research paper thumbnail of Novel gaseous ethylene inhibitor prevents ethylene effects in potted flowering plants

Research paper thumbnail of Plant Regeneration from Different Explant Types of Streptocarpus Spec. In Vitro

Acta Horticulturae, 2006

In order to develop an in vitro regeneration protocol for different Streptocarpus species, we inv... more In order to develop an in vitro regeneration protocol for different Streptocarpus species, we investigated the effects of three factors: surface sterilization, regeneration medium and explant type. For surface sterilization the influence of an ethanol (70%) treatment for 30 s was tested, before the explants were immersed in 1.5% sodium hypochlorite for 10 min. Our experiments showed no significant difference regarding contamination of explants between the surface sterilization with and without ethanol. On the other hand, the treatment with 70% ethanol led to damage of the leaf explants regarding regeneration, especially the number of shootlets. The variation of indole acetic acid (IAA) and benzylaminopurine (BAP) concentrations in the regeneration medium revealed, that a combination of 1.0 mg L IAA and 1.0 mg L -1 BAP was most efficient for shoot regeneration in three species (S1, S3, S4). However, the best quality and regeneration response of the fourth species (S7) was obtained with 1.0 mg L -1 IAA and 0.1 mg L -1 BAP. The differences of the most suitable combination of phytohormones showed, that it was not possible to designate a uniform optimal medium for all of Streptocarpus species. In addition, different explant types were compared. Taking into consideration the regeneration intensity, the expenditure of time for explant preparation and material costs, we concluded that the most suitable explant type were leaf blade explants.

Research paper thumbnail of Efficiency of Pmi/Mannose as a Selectable Marker for Transformation of Kalanchoe

Acta Horticulturae, 2012

ABSTRACT Mannose is an unsuitable carbon source for many plants. Various factors were studied to ... more ABSTRACT Mannose is an unsuitable carbon source for many plants. Various factors were studied to evaluate the efficacy of mannose in the for production of transgenic plants using Agrobacterium tumefaciens-mediated transformation of leaf explants of Kalanchoe blossfeldiana. To establish a non-antibiotic selection system that utilizes the phosphomannose-isomerase (PMI) gene from E. coli, we determined the optimum mannose concentration for selecting transformed cells. Shoot organogenesis in K. blossfeldiana leaf explants was suppressed effectively on medium supplemented with mannose, or mannose with glucose, which indicated that Kalanchoe cells had little or no PMI activity and could not utilize mannose as a carbon source. The optimum concentration of mannose was found to eliminate non-transgenic shoots. Transformed plants were confirmed by GUS assay. Transformation ranged up to ca. 40%. The PMI/mannose system provides an efficient method for selecting without antibiotics or herbicides.

Research paper thumbnail of Plant Regeneration via Somatic Embryogenesis in Schlumbergera Truncate

Research paper thumbnail of Enhanced Regenerative Capacity in Rhipsalidopsis and Schlumbergera Explants by in Vitro Manipulations

Acta Horticulturae, 2006

Several plant species are recalcitrant when considering regeneration through adventitious organs.... more Several plant species are recalcitrant when considering regeneration through adventitious organs. Establishment of regeneration systems for the plants of interest is a pre-requisite for further work such as genetic transformation and induced mutation. The cactus plants belonging to species Schlumbergera and Rhipsalidopsis were found to be recalcitrant. In addition to this problem, they were very slow to grow. However, it was possible to achieve high regenerative ability in explants of in vitro cultures of these cacti. The initial phylloclade explants established from adult plants grown in greenhouse were found to be recalcitrant. These explants produced only axillary shoots from the pre-formed shoot primodia when treated with exogenous plant hormones, but they failed to produce any adventitious shoots. There was a significant improvement in adventitious shoot formation in phylloclade explants, more particularly, of Rhipsalidopsis in long-term cultures. After the first subculture, sporadic adventitious shoot formation began, and after the third subculture 87% explants of one of the cultiyars of Rhipsalidopsis produced adventitious shoots. There was an increase in the regenerative capacity in the explants of Schlumbergera, however, the efficiency was not to the same extent as for the other species. Callus cultures were found to be more regenerative compared to the phylloclade explants. The results clearly indicated that the in vitro manipulations helped in increasing the capacity to regenerate.

Research paper thumbnail of Effects and Interactions of Ethylene and Abscisic Acid in Detached and Intact Miniature Rose Flowers

IV International Conference on Postharvest Science, 2001

Research paper thumbnail of Physiological and Genetic Reasons for Different Postharvest Characteristics in Miniature Potted Roses

VII International Symposium on Postharvest Physiology of Ornamental Plants, 2001

Research paper thumbnail of Zakizadeh, H., Lütken H., Sriskandarajah S., M. Serek and R. Müller (2012) Transformation of miniature potted rose (Rosa hybrida cv. Linda) with PSAG12-ipt gene enhances resistance to leaf senescence and exogenous ethylene. Plant Cell Reports (Online, Dec 2012 :DOI 10.1007/s00299-012-1354-5)

Research paper thumbnail of Chemical Compounds Interacting with the Ethylene Receptor in Ornamental Crops

X International Symposium on Postharvest Quality of Ornamental Plants, 2015

Research paper thumbnail of Molecular Genetic Investigation of Malformed Flower Types of Rosa Hybrid L

XXIV International Eucarpia Symposium Section Ornamentals: Ornamental Breeding Worldwide, 2012