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Papers by M. Sharifi-yazdi

Archive of SID A NEW VARIETY AND A REVIEW OF THE GENUS NEOTORULARIA (BRASSICACEAE) IN FLORA OF IRAN

The Journal of Qazvin University of Medical Sciences, 2016

Background: Diarrhea is one of the most important causes of children mortality in developing coun... more Background: Diarrhea is one of the most important causes of children mortality in developing countries. Gastroenteritis causes mortality in children younger than five years old annually. The presence of antibiotics resistance species among these bacteria are major health problems. Objective: This study aimed to determine the antibiotic susceptibility and MIC50%, MIC90% of Yersinia to three beta-lactam antibiotics. Methods: This experimental study was determined sensitivity of different species of Yersinia to three beta-lactam antibiotics (Ampicillin-Cefazolin Cefotaxime ). In total 50 species (39 from different human sources, environments, foods and 11 from Pasteur institutions in Paris) were used. Minimum inhibitory concentration (MIC) was carried out by serial dilution method. Findings: Among three tested antibiotics, the lowest MIC 50%, MIC 90% were obtained for Cefotaxime (≤0.125μg/ml and 0.5μg/ml). All the tested species showed resistance to Ampicillin and very low sensitivity ...

Journal of Clinical Microbiology and Biochemical Technology, 2017

Background & Aim: Despite the drug resistance M.bovis and Mycobacterium tuberculosis (MTB) are st... more Background & Aim: Despite the drug resistance M.bovis and Mycobacterium tuberculosis (MTB) are still regarded as two of the global health problems in the world. In the present study, a comparison was made between protein profi les of M.bovis and MTB in order to achieve effective biomarkers for diagnosis of TB. The clinical samples, sputum and gastric lavage (and the other samples) were processed by N-acetyl-Lcysteine-sodium hydroxide methods and consequently were cultured on Lowenstein-Jensen medium. Mycobacterium tuberculosis and bovis strains were distinguished according to the biochemical tests and susceptibility testing system. Colonies were grown in 7H9 medium and membrane and secretory proteins were extracted, purifi ed by ammonium sulfate and refrigerated alcohol methods. The protein contents were measured by Bradford method. Comparison of protein bands in each strain were performed by one dimensional electrophoresis. The major discrepancy between the two strains in the banding separation membrane proteins could be observed in 45 and 60 KDa and also less than 45 and 14 KDa. The results showed that discrepancy in the proteins bands could be used as protein effective biomarker for TB diagnosis. We should use antibody against TB for further investigation for rapid TB diagnosis

Archives of Disease in Childhood, 2012

Iranian Journal of Public Health, 2002

The immounological responses to S. aureus cell wall antigens, namely crude or whole cell, teichoi... more The immounological responses to S. aureus cell wall antigens, namely crude or whole cell, teichoic acid (TA) peptidoglycan prepared in conventional way (CPG), cell wall (CW), lipoteichoic acid (LTA) were investigated by number of immunological methods e.g. RIA, ELISA, immunodiffusion and western blotting techniques.Also a method for preparation of peptidoglycan (MPG) was modified and used as vaccine for our protection studies. In general the responses in rabbits (serum titre) to fractions of S. aureus, varied, crued antigen showed the highest end-point titer while lipoteichoic acid showed the lowest end-point. The highest end-point titre was always seen between homologous sera and antigens. Modified pepdidoglycan and cell wall (PG + TA) showed the similar titre against S. epidermidis hyperimmueserum. The cross-reactivty was also seen between fractions by S. aureus Bate. S. aureus Wood (lack protein A) S. epidermidis and streptococci against various immune sera tested by Elisa and gel diffiusion. The results demonstrated that protective antibody can be obtained between different strain or species of bacteria due to the presence of common antigen or antigenes especially PG in gram-positive bacteria.

World Journal of Microbiology and Biotechnology, 2012

Lactobacillus plantarum strains isolated and identified from naturally-fermented Chinese sauerkra... more Lactobacillus plantarum strains isolated and identified from naturally-fermented Chinese sauerkraut were examined in vitro for potential probiotic properties and in vivo for cholesterol-lowering effect in mice. Among 7 isolated L. plantarum strains, strains S2-5 and S4-1 were found to possess desirable probiotic properties including ability to survive at pH 2.0 for 60 min, tolerate pancreatin and bile salts, adhere to Caco-2 cells, produce high b-galactosidase activity and antimicrobial activity against Escherichia coli O157 and Shigella flexneri CMCC(B). In addition, strains S2-5 and S4-1 were susceptible to several antibiotics, and capable of reducing cholesterol level in MRS medium by assimilation of cholesterol at 20.39 and 22.28 lg ml-1 , respectively. The in vivo study with L. plantarum S4-1 showed that feeding with fermented milk containing this strain was able to effectively reduce serum cholesterol level in mice, demonstrating its potential as an excellent probiotic candidate for applications in functional products.

Combining ability of male and female genotypes of pistacia atlantica trees in 9 different provenances

(English) In 2003, some pistacia atlantica trees from 9 different provenances including Ilam, Ker... more (English) In 2003, some pistacia atlantica trees from 9 different provenances including Ilam, Kerman, Kermanshah, Khaklun, Mamand, Prikdan, Shiraz, Sistan & Yazd were selected to obtain half sib seeds. At harvesting time, the seeds were collected and ...

Evaluation of Antibiotic Susceptibility of Enterobacteriacea Isolates from Blood Cultures

African Journal of Microbiology Research, 2011

Listeria monocytogenes is a causative agent of listeriosis. Clinical manifestation of invasive li... more Listeria monocytogenes is a causative agent of listeriosis. Clinical manifestation of invasive listeriosis is usually sever and include sepsis and meningoencephalitis. Uterian infection in listeriosis depend on gestational age varies from preterm labour, delivery of infected neonate to abortion. The present study investigated the incidence and antibiograms of the organism in clinical samples were collected from women with spontaneous abortions in Iran. Clinical samples were collected from patients with spontaneous abortions hospitalized in Shariati hospital in Tehran during December 2009 and November 2010. Antibiogram was done by disk-agar method. L. monocytogenes was isolated from 9 of 100 samples. Out of 9 isolates of L. monocytogenes 3 (16.66%), 0 (0%), 1 (4%), 3 (12%) and 2 (8%) were isolated from placental tissue, blood, urine, vaginal and rectal swabs respectively. Resistance to Penicillin G had the highest rate. Because of high incidence of L. monocytogenes in this study, in women with history of abortions or bad obstetric history, diagnosis procedures for detection of L. monocytogenes and on time treatment is recommended additionally because of high resistance rate of bacteria to Penicillin G in this study, antibacterial susceptibility before initiation of treatment is recommended.

The activated sludge process simply involves bringing together wastewater and a mixed culture of ... more The activated sludge process simply involves bringing together wastewater and a mixed culture of microorganisms under aerobic conditions. The system usually includes a secondary treatment given to the settled sewage, and requires an environment in which active microorganisms are maintained in intimate contact with wastewater in the presence of sufficient oxygen. In this study, the treatment of industrial effluents, by using laboratory activated sludge unit was investigated. The reduction of the pollution laws was determined, using the biochemical oxygen demand (BOD) and the chemical oxygen demand (COD) methods. The results indicated that the pollution laws was reduced by up to 98% in the activated sludge unit.

Inhibitory effect of Lactobacillus plantarum K-1 on passive cutaneous anaphylaxis reaction and scratching behavior in mice

Archives of Pharmacal Research, 2011

Lactobacillus plantarum K-1 (LP) inhibiting AP-1 (c-Jun) and NF-κB activations was isolated from ... more Lactobacillus plantarum K-1 (LP) inhibiting AP-1 (c-Jun) and NF-κB activations was isolated from kimchi, and its inhibitory activity against scratching behavior and passive cutaneous anaphylaxis reaction in mice was investigated. Heat-inactivated LP (heated at 60°C for 30 min) potently inhibited the expression of TNF-α and IL-4 as well as the activation of their transcription factors, NF-κB and c-jun, in phorbol 12'-myristate 13'-acetate-stimulated RBL-2H3 cells. LP (1 × 10(10) CFU per mouse) showed a potent inhibition against passive cutaneous anaphylaxis reaction induced by the IgE-antigen complex in mice, inhibiting it by 87.5%. LP (1 × 10(10) CFU/mouse) inhibited histamine-induced scratching behavior by 58.9% compared to the control group. LP significantly inhibited vascular permeability induced by histamine. The inhibitory activity of LP against vascular permeability was in proportion to its inhibition against scratching behavior. LP potently inhibited histamine-induced cytokine production: it (1 × 10(10) CFU per mouse) inhibited IL-4, IL-1β, and TNF-α expression by 88.9%, 88.6%, and 98.9%, respectively. LP also inhibited IgE level increased by histamine by 85.3%. It inhibited histamine-induced the activations of their transcription factors, NF-κB and c-Jun. Based on these findings, LP may improve allergic diseases, such as anaphylaxis, atopic dermatitis, rhinitis, and pruritus by inhibiting the expression of IgE-switching cytokine IL-4 and proinflammatory cytokines IL-1β and TNF-α via NF-κB and AP-1 signaling pathways.

Journal of Payavard Salamat, 2007

1 . نارهت يكشزپ مولع هاگشناد يكشزپاريپ هدكشناد يهاگشيامزآ مولع هورگ يبرم 2 . نارهت يكشزپ مولع هاگ... more 1 . نارهت يكشزپ مولع هاگشناد يكشزپاريپ هدكشناد يهاگشيامزآ مولع هورگ يبرم 2 . نارهت يكشزپ مولع هاگشناد يژولوتامه دشرا يسانشراك 3 . رايشناد مولع هورگ يژولويبوركيم آ نارهت يكشزپ مولع هاگشناد يكشزپاريپ هدكشناد يهاگشيامز 4 . هورگ رايداتسا آ شناد يژولونوميا يشزوم نارهت يكشزپ مولع هاگشناد تشادهب هدك 5 . رايداتسا نارهت يكشزپ مولع هاگشناد يكشزپاريپ هدكشناد ينامرد يتشادهب تامدخ تيريدمو يژولويب 6 . سردم تيبرت هاگشناد يكشزپ هدكشناد يژولونوميا هورگ يژولوتامهونوميا داتسا هديكچ فده و هنيمز : ياهيراميب نيرتعياش زا يكي يمسلاات يراميب تسا ناسنا يكيتنژ . ايند طاقن مامت رد يمسلاات ياه نژ , يور ياهروشك رد صوصخب تسا عياش ناريا هلمج زا يمسلاات دنبرمك . اب هجيتن رد نوخ تفايرد تهج ناراميب نيا مربم زاين و ناريا رد يكيتنژ يراميب نيا ندوب عياش هب هجوت اب فده اب روژام يمسلاات ناراميب يكيژولورس يسررب تفرگ ماجنا هعلاطم نيا سوريوولاگموتيس لاعف تنوفع ظاحل زا رركم نوخ تفايرد . يسررب شور : دش ماجنا يعطقم شور هب هك هعلاطم نيارد 309 ريز يمسلاات ناراميب زا رفن 15 فلتخم ينامرد زكارم زا رركم تروص هب هك لاس باختنا دندرك يم...

Background and Objective: The most common enterohemorrhagic Escherichia coli strain is the O157: ... more Background and Objective: The most common enterohemorrhagic Escherichia coli strain is the O157: H7 serotype, which is one of the most important intestinal pathogens and can cause complications such as hemorrhagic colitis, hemolytic uremic syndrome and acute renal failure. The aim of this study was to evaluate the enterohemorrhagic Escherichia coli causing molecular outbreaks of foodborne illness in Iran. Methods: In this descriptive cross-sectional study, 189 fecal swab specimens were examined during April to September 2018. All suspected isolates were tested for biochemical tests. The isolates were confirmed by molecular PCR and evaluated by antimicrobial susceptibility tests. Results: From 189 stool swab samples studied, 98 Escherichia coli isolates were detected based on phenotypic tests. Most of the outbreaks occurred in summer and the prevalence of enterohemorrhagic Escherichia coli was 24.5%, which 4% of them were non-O157H7. Most patients were between 1 and 12 years of age a...

The Frequency of Extended Spectrum Beta Lactamase and CTX M-I of Escherichia Coli Isolated from the Urine Tract Infection of Patients by Phenotypic and PCR Methods in the City of Khoy in Iran

Background and Objective: The production of Extended Spectrum Beta Lactamases (ESBLs) by Escheric... more Background and Objective: The production of Extended Spectrum Beta Lactamases (ESBLs) by Escherichia coli is the main cause of resistance to Cephalosporins. In the past decade, CTX-M enzymes have become the most prevalent ESBLs in Europe, Canada, and Asia. In this study, the frequency of ESBL-producing E.coli and molecular detection of the CTX-M-I group was investigated. Materials and Methods: A total of 400 urine samples were collected from both hospitalized and out-patients in Khoys hospitals between November 2009 and April 2010. Out of these samples, 188 were identified as E.coli by standard biochemical tests. The antibiotic Susceptibility tests to 10 antibiotics were performed by the-disk-agar diffusion (DAD) method. ESBL production was screened by phenotypic test that including disk diffusion agar and combined disk as recommended by the Clinical and Laboratory Standards Institute (CLSI). Screened isolates were investigated by PCR assay for detection of CTX-M-I group genes. Resu...

Autecology of Ferula oopoda (Boiss & Buhse)

Iranian Journal of Range and Desert Research, 2009

Journal of Gorgan University of Medical Sciences, 2014

Background and Objective: Gastroenteritis due to Salmonella is common in human and considered as ... more Background and Objective: Gastroenteritis due to Salmonella is common in human and considered as a global dilemma of public health. This study was done to determine the Pattern of serotyping and antibiotic resistance of Salmonella in children with diarrhea in Iran. Methods: In this laboratory study, 306 stool samples were collected from children with diarrhea in public health centers in Robat-karim, Tehran province, Iran. The specimens were enriched in Selenite F medium and then cultured on Hekton agar. The identification of Salmonella was carried out by conventional method and antimicrobial susceptibility testing was performed according to CLSI procedures. Results: Out of 306 stool samples, 7.2 % were identified as Salmonella species, as follow: 7 Salmonella typhi, 6 Salmonella paratyphi B, 3 Salmonella paratyphi C, 2 Salmonella paratyphi A and 4 samples were not identifiable. There was a significant relation between presence of WBC in fecal and salmonellosis (P<0.05). In drug s...

Comparison of Multiple Antibiotic Resistance Patterns of Klebsiella Bacteria Groups Causing Urinary Infections and Determination of Imipenem MIC in MDR Strains

Comparison of Selective Media for the Detection of Escherichia Coli in Water

Iranian Journal of Public Health, 1999

Archive of SID A NEW VARIETY AND A REVIEW OF THE GENUS NEOTORULARIA (BRASSICACEAE) IN FLORA OF IRAN

The Journal of Qazvin University of Medical Sciences, 2016

Background: Diarrhea is one of the most important causes of children mortality in developing coun... more Background: Diarrhea is one of the most important causes of children mortality in developing countries. Gastroenteritis causes mortality in children younger than five years old annually. The presence of antibiotics resistance species among these bacteria are major health problems. Objective: This study aimed to determine the antibiotic susceptibility and MIC50%, MIC90% of Yersinia to three beta-lactam antibiotics. Methods: This experimental study was determined sensitivity of different species of Yersinia to three beta-lactam antibiotics (Ampicillin-Cefazolin Cefotaxime ). In total 50 species (39 from different human sources, environments, foods and 11 from Pasteur institutions in Paris) were used. Minimum inhibitory concentration (MIC) was carried out by serial dilution method. Findings: Among three tested antibiotics, the lowest MIC 50%, MIC 90% were obtained for Cefotaxime (≤0.125μg/ml and 0.5μg/ml). All the tested species showed resistance to Ampicillin and very low sensitivity ...

Journal of Clinical Microbiology and Biochemical Technology, 2017

Background & Aim: Despite the drug resistance M.bovis and Mycobacterium tuberculosis (MTB) are st... more Background & Aim: Despite the drug resistance M.bovis and Mycobacterium tuberculosis (MTB) are still regarded as two of the global health problems in the world. In the present study, a comparison was made between protein profi les of M.bovis and MTB in order to achieve effective biomarkers for diagnosis of TB. The clinical samples, sputum and gastric lavage (and the other samples) were processed by N-acetyl-Lcysteine-sodium hydroxide methods and consequently were cultured on Lowenstein-Jensen medium. Mycobacterium tuberculosis and bovis strains were distinguished according to the biochemical tests and susceptibility testing system. Colonies were grown in 7H9 medium and membrane and secretory proteins were extracted, purifi ed by ammonium sulfate and refrigerated alcohol methods. The protein contents were measured by Bradford method. Comparison of protein bands in each strain were performed by one dimensional electrophoresis. The major discrepancy between the two strains in the banding separation membrane proteins could be observed in 45 and 60 KDa and also less than 45 and 14 KDa. The results showed that discrepancy in the proteins bands could be used as protein effective biomarker for TB diagnosis. We should use antibody against TB for further investigation for rapid TB diagnosis

Archives of Disease in Childhood, 2012

Iranian Journal of Public Health, 2002

The immounological responses to S. aureus cell wall antigens, namely crude or whole cell, teichoi... more The immounological responses to S. aureus cell wall antigens, namely crude or whole cell, teichoic acid (TA) peptidoglycan prepared in conventional way (CPG), cell wall (CW), lipoteichoic acid (LTA) were investigated by number of immunological methods e.g. RIA, ELISA, immunodiffusion and western blotting techniques.Also a method for preparation of peptidoglycan (MPG) was modified and used as vaccine for our protection studies. In general the responses in rabbits (serum titre) to fractions of S. aureus, varied, crued antigen showed the highest end-point titer while lipoteichoic acid showed the lowest end-point. The highest end-point titre was always seen between homologous sera and antigens. Modified pepdidoglycan and cell wall (PG + TA) showed the similar titre against S. epidermidis hyperimmueserum. The cross-reactivty was also seen between fractions by S. aureus Bate. S. aureus Wood (lack protein A) S. epidermidis and streptococci against various immune sera tested by Elisa and gel diffiusion. The results demonstrated that protective antibody can be obtained between different strain or species of bacteria due to the presence of common antigen or antigenes especially PG in gram-positive bacteria.

World Journal of Microbiology and Biotechnology, 2012

Lactobacillus plantarum strains isolated and identified from naturally-fermented Chinese sauerkra... more Lactobacillus plantarum strains isolated and identified from naturally-fermented Chinese sauerkraut were examined in vitro for potential probiotic properties and in vivo for cholesterol-lowering effect in mice. Among 7 isolated L. plantarum strains, strains S2-5 and S4-1 were found to possess desirable probiotic properties including ability to survive at pH 2.0 for 60 min, tolerate pancreatin and bile salts, adhere to Caco-2 cells, produce high b-galactosidase activity and antimicrobial activity against Escherichia coli O157 and Shigella flexneri CMCC(B). In addition, strains S2-5 and S4-1 were susceptible to several antibiotics, and capable of reducing cholesterol level in MRS medium by assimilation of cholesterol at 20.39 and 22.28 lg ml-1 , respectively. The in vivo study with L. plantarum S4-1 showed that feeding with fermented milk containing this strain was able to effectively reduce serum cholesterol level in mice, demonstrating its potential as an excellent probiotic candidate for applications in functional products.

Combining ability of male and female genotypes of pistacia atlantica trees in 9 different provenances

(English) In 2003, some pistacia atlantica trees from 9 different provenances including Ilam, Ker... more (English) In 2003, some pistacia atlantica trees from 9 different provenances including Ilam, Kerman, Kermanshah, Khaklun, Mamand, Prikdan, Shiraz, Sistan & Yazd were selected to obtain half sib seeds. At harvesting time, the seeds were collected and ...

Evaluation of Antibiotic Susceptibility of Enterobacteriacea Isolates from Blood Cultures

African Journal of Microbiology Research, 2011

Listeria monocytogenes is a causative agent of listeriosis. Clinical manifestation of invasive li... more Listeria monocytogenes is a causative agent of listeriosis. Clinical manifestation of invasive listeriosis is usually sever and include sepsis and meningoencephalitis. Uterian infection in listeriosis depend on gestational age varies from preterm labour, delivery of infected neonate to abortion. The present study investigated the incidence and antibiograms of the organism in clinical samples were collected from women with spontaneous abortions in Iran. Clinical samples were collected from patients with spontaneous abortions hospitalized in Shariati hospital in Tehran during December 2009 and November 2010. Antibiogram was done by disk-agar method. L. monocytogenes was isolated from 9 of 100 samples. Out of 9 isolates of L. monocytogenes 3 (16.66%), 0 (0%), 1 (4%), 3 (12%) and 2 (8%) were isolated from placental tissue, blood, urine, vaginal and rectal swabs respectively. Resistance to Penicillin G had the highest rate. Because of high incidence of L. monocytogenes in this study, in women with history of abortions or bad obstetric history, diagnosis procedures for detection of L. monocytogenes and on time treatment is recommended additionally because of high resistance rate of bacteria to Penicillin G in this study, antibacterial susceptibility before initiation of treatment is recommended.

The activated sludge process simply involves bringing together wastewater and a mixed culture of ... more The activated sludge process simply involves bringing together wastewater and a mixed culture of microorganisms under aerobic conditions. The system usually includes a secondary treatment given to the settled sewage, and requires an environment in which active microorganisms are maintained in intimate contact with wastewater in the presence of sufficient oxygen. In this study, the treatment of industrial effluents, by using laboratory activated sludge unit was investigated. The reduction of the pollution laws was determined, using the biochemical oxygen demand (BOD) and the chemical oxygen demand (COD) methods. The results indicated that the pollution laws was reduced by up to 98% in the activated sludge unit.

Inhibitory effect of Lactobacillus plantarum K-1 on passive cutaneous anaphylaxis reaction and scratching behavior in mice

Archives of Pharmacal Research, 2011

Lactobacillus plantarum K-1 (LP) inhibiting AP-1 (c-Jun) and NF-κB activations was isolated from ... more Lactobacillus plantarum K-1 (LP) inhibiting AP-1 (c-Jun) and NF-κB activations was isolated from kimchi, and its inhibitory activity against scratching behavior and passive cutaneous anaphylaxis reaction in mice was investigated. Heat-inactivated LP (heated at 60°C for 30 min) potently inhibited the expression of TNF-α and IL-4 as well as the activation of their transcription factors, NF-κB and c-jun, in phorbol 12&amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;#39;-myristate 13&amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;#39;-acetate-stimulated RBL-2H3 cells. LP (1 × 10(10) CFU per mouse) showed a potent inhibition against passive cutaneous anaphylaxis reaction induced by the IgE-antigen complex in mice, inhibiting it by 87.5%. LP (1 × 10(10) CFU/mouse) inhibited histamine-induced scratching behavior by 58.9% compared to the control group. LP significantly inhibited vascular permeability induced by histamine. The inhibitory activity of LP against vascular permeability was in proportion to its inhibition against scratching behavior. LP potently inhibited histamine-induced cytokine production: it (1 × 10(10) CFU per mouse) inhibited IL-4, IL-1β, and TNF-α expression by 88.9%, 88.6%, and 98.9%, respectively. LP also inhibited IgE level increased by histamine by 85.3%. It inhibited histamine-induced the activations of their transcription factors, NF-κB and c-Jun. Based on these findings, LP may improve allergic diseases, such as anaphylaxis, atopic dermatitis, rhinitis, and pruritus by inhibiting the expression of IgE-switching cytokine IL-4 and proinflammatory cytokines IL-1β and TNF-α via NF-κB and AP-1 signaling pathways.

Journal of Payavard Salamat, 2007

1 . نارهت يكشزپ مولع هاگشناد يكشزپاريپ هدكشناد يهاگشيامزآ مولع هورگ يبرم 2 . نارهت يكشزپ مولع هاگ... more 1 . نارهت يكشزپ مولع هاگشناد يكشزپاريپ هدكشناد يهاگشيامزآ مولع هورگ يبرم 2 . نارهت يكشزپ مولع هاگشناد يژولوتامه دشرا يسانشراك 3 . رايشناد مولع هورگ يژولويبوركيم آ نارهت يكشزپ مولع هاگشناد يكشزپاريپ هدكشناد يهاگشيامز 4 . هورگ رايداتسا آ شناد يژولونوميا يشزوم نارهت يكشزپ مولع هاگشناد تشادهب هدك 5 . رايداتسا نارهت يكشزپ مولع هاگشناد يكشزپاريپ هدكشناد ينامرد يتشادهب تامدخ تيريدمو يژولويب 6 . سردم تيبرت هاگشناد يكشزپ هدكشناد يژولونوميا هورگ يژولوتامهونوميا داتسا هديكچ فده و هنيمز : ياهيراميب نيرتعياش زا يكي يمسلاات يراميب تسا ناسنا يكيتنژ . ايند طاقن مامت رد يمسلاات ياه نژ , يور ياهروشك رد صوصخب تسا عياش ناريا هلمج زا يمسلاات دنبرمك . اب هجيتن رد نوخ تفايرد تهج ناراميب نيا مربم زاين و ناريا رد يكيتنژ يراميب نيا ندوب عياش هب هجوت اب فده اب روژام يمسلاات ناراميب يكيژولورس يسررب تفرگ ماجنا هعلاطم نيا سوريوولاگموتيس لاعف تنوفع ظاحل زا رركم نوخ تفايرد . يسررب شور : دش ماجنا يعطقم شور هب هك هعلاطم نيارد 309 ريز يمسلاات ناراميب زا رفن 15 فلتخم ينامرد زكارم زا رركم تروص هب هك لاس باختنا دندرك يم...

Background and Objective: The most common enterohemorrhagic Escherichia coli strain is the O157: ... more Background and Objective: The most common enterohemorrhagic Escherichia coli strain is the O157: H7 serotype, which is one of the most important intestinal pathogens and can cause complications such as hemorrhagic colitis, hemolytic uremic syndrome and acute renal failure. The aim of this study was to evaluate the enterohemorrhagic Escherichia coli causing molecular outbreaks of foodborne illness in Iran. Methods: In this descriptive cross-sectional study, 189 fecal swab specimens were examined during April to September 2018. All suspected isolates were tested for biochemical tests. The isolates were confirmed by molecular PCR and evaluated by antimicrobial susceptibility tests. Results: From 189 stool swab samples studied, 98 Escherichia coli isolates were detected based on phenotypic tests. Most of the outbreaks occurred in summer and the prevalence of enterohemorrhagic Escherichia coli was 24.5%, which 4% of them were non-O157H7. Most patients were between 1 and 12 years of age a...

The Frequency of Extended Spectrum Beta Lactamase and CTX M-I of Escherichia Coli Isolated from the Urine Tract Infection of Patients by Phenotypic and PCR Methods in the City of Khoy in Iran

Background and Objective: The production of Extended Spectrum Beta Lactamases (ESBLs) by Escheric... more Background and Objective: The production of Extended Spectrum Beta Lactamases (ESBLs) by Escherichia coli is the main cause of resistance to Cephalosporins. In the past decade, CTX-M enzymes have become the most prevalent ESBLs in Europe, Canada, and Asia. In this study, the frequency of ESBL-producing E.coli and molecular detection of the CTX-M-I group was investigated. Materials and Methods: A total of 400 urine samples were collected from both hospitalized and out-patients in Khoys hospitals between November 2009 and April 2010. Out of these samples, 188 were identified as E.coli by standard biochemical tests. The antibiotic Susceptibility tests to 10 antibiotics were performed by the-disk-agar diffusion (DAD) method. ESBL production was screened by phenotypic test that including disk diffusion agar and combined disk as recommended by the Clinical and Laboratory Standards Institute (CLSI). Screened isolates were investigated by PCR assay for detection of CTX-M-I group genes. Resu...

Autecology of Ferula oopoda (Boiss & Buhse)

Iranian Journal of Range and Desert Research, 2009

Journal of Gorgan University of Medical Sciences, 2014

Background and Objective: Gastroenteritis due to Salmonella is common in human and considered as ... more Background and Objective: Gastroenteritis due to Salmonella is common in human and considered as a global dilemma of public health. This study was done to determine the Pattern of serotyping and antibiotic resistance of Salmonella in children with diarrhea in Iran. Methods: In this laboratory study, 306 stool samples were collected from children with diarrhea in public health centers in Robat-karim, Tehran province, Iran. The specimens were enriched in Selenite F medium and then cultured on Hekton agar. The identification of Salmonella was carried out by conventional method and antimicrobial susceptibility testing was performed according to CLSI procedures. Results: Out of 306 stool samples, 7.2 % were identified as Salmonella species, as follow: 7 Salmonella typhi, 6 Salmonella paratyphi B, 3 Salmonella paratyphi C, 2 Salmonella paratyphi A and 4 samples were not identifiable. There was a significant relation between presence of WBC in fecal and salmonellosis (P<0.05). In drug s...

Comparison of Multiple Antibiotic Resistance Patterns of Klebsiella Bacteria Groups Causing Urinary Infections and Determination of Imipenem MIC in MDR Strains

Comparison of Selective Media for the Detection of Escherichia Coli in Water

Iranian Journal of Public Health, 1999