M. Maccaferri - Academia.edu (original) (raw)

Papers by M. Maccaferri

Crop stress management and global climate change, 2011

... et al., 2010). However, it should be noted that other studies in rice have reported a weak or... more ... et al., 2010). However, it should be noted that other studies in rice have reported a weak or even absence of correlation between root features and drought resistance (Pantuwan et al., 2002; Subashri et al., 2009). The final effects ...

Annals of botany, 2002

Comparative analysis of a number of studies in drought-stressed maize (Zea mays L.) reporting qua... more Comparative analysis of a number of studies in drought-stressed maize (Zea mays L.) reporting quantitative trait loci (QTLs) for abscisic acid concentration, root characteristics, other morpho-physiological traits (MPTs) and grain yield (GY) reveals their complex genetic basis and the influence of the genetic background and the environment on QTL effects. Chromosome regions (e.g. near umc11 on chromosome 1 and near csu133 on chromosome 2) with QTLs controlling a number of MPTs and GY across populations and conditions of different water supply have been identified. Examples are presented on the use of QTL information to elucidate the genetic and physiological bases of the association among MPTs and GY. The QTL approach allows us to develop hypotheses accounting for these associations which can be further tested by developing near isogenic lines (NILs) differing for the QTL alleles. NILs also allow for a more accurate assessment of the breeding value of MPTs and, in some cases, may al...

Fibrinolysis, 1990

Summary The traditional assays for measuring fibrin/ogen degradation products (FDP) have limitati... more Summary The traditional assays for measuring fibrin/ogen degradation products (FDP) have limitations due to the use of serum, and do not discriminate between FDPs deriving from fibrin or fibrinogen. The crosslinked fibrin derivatives may be detected by means of ...

Fibrinolysis, 1988

Abstract Fibrinolysis and related parameters were studied before and after a venous occlusion tes... more Abstract Fibrinolysis and related parameters were studied before and after a venous occlusion test in a group of obese non diabetic children, in comparison with an age and sex-matched control group. Obese children (exceeding by at least 25% the upper limit of ...

Genetic analysis of SBCMV resistance in durum wheat was carried out using a population of 181 rec... more Genetic analysis of SBCMV resistance in durum wheat was carried out using a population of 181 recombinant inbred lines (RILs) obtained from Meridiano (moderately resistant) × Claudio (moderately susceptible). The RILs were characterized for SBCMV response in the field under severe and uniform SBCMV infection during 2007 and 2008 and profiled with SSR and DArT markers. A wide range of

BMC Genomics, 2014

Durum wheat (Triticum durum Desf.) is a tetraploid cereal grown in the medium to low-precipitatio... more Durum wheat (Triticum durum Desf.) is a tetraploid cereal grown in the medium to low-precipitation areas of the Mediterranean Basin, North America and South-West Asia. Genomics applications in durum wheat have the potential to boost exploitation of genetic resources and to advance understanding of the genetics of important complex traits (e.g. resilience to environmental and biotic stresses). A dense and accurate consensus map specific for T. durum will greatly facilitate genetic mapping, functional genomics and marker-assisted improvement. High quality genotypic data from six core recombinant inbred line populations were used to obtain a consensus framework map of 598 simple sequence repeats (SSR) and Diversity Array Technology® (DArT) anchor markers (common across populations). Interpolation of unique markers from 14 maps allowed us to position a total of 2,575 markers in a consensus map of 2,463 cM. The T. durum A and B genomes were covered in their near totality based on the reference SSR hexaploid wheat map. The consensus locus order compared to those of the single component maps showed good correspondence, (average Spearman's rank correlation rho ρ value of 0.96). Differences in marker order and local recombination rate were observed between the durum and hexaploid wheat consensus maps. The consensus map was used to carry out a whole-genome search for genetic differentiation signatures and association to heading date in a panel of 183 accessions adapted to the Mediterranean areas. Linkage disequilibrium was found to decay below the r2 threshold=0.3 within 2.20 cM, on average. Strong molecular differentiations among sub-populations were mapped to 87 chromosome regions. A genome-wide association scan for heading date from 27 field trials in the Mediterranean Basin and in Mexico yielded 50 chromosome regions with evidences of association in multiple environments. The consensus map presented here was used as a reference for genetic diversity and mapping analyses in T. durum, providing nearly complete genome coverage and even marker density. Markers previously mapped in hexaploid wheat constitute a strong link between the two species. The consensus map provides the basis for high-density single nucleotide polymorphic (SNP) marker implementation in durum wheat.

Fibrinolysis, 1990

Plasminogen activator inhibitor (PA&l) antigen and fibrinjogen degradation products (FbDP, FgDP) ... more Plasminogen activator inhibitor (PA&l) antigen and fibrinjogen degradation products (FbDP, FgDP) were measured before and 8 days after gynaecological surgery in 50 patients randomly submitted to prophylaxis with low molecular weight heparin (Alfa LMWl-OP 2123, Alfa Wassermann, Bologna, Italy; LMWH group) or standard calcium heparin (CH group). All patients were screened for deep venous thrombosis by the 1251 Fibrinogen uptake test. In the CH group, but not in LMWH group, postoperative PAI-levels were significantly higher (~~0.01) than at baseline. The percent variation of PAI-between pre-and postoperative values was significantly higher in the CH than in LMWH group (~~0.01). Postoperative FbDP values were significantly increased in both groups (~~0.01) but FgDP only in LMWH group (pcO.01). No significant differences in pre-and postoperative PAI-values were found between patients with positive or negative "'1 Fibrinogen uptake test.

Plant Biotechnology Journal, 2014

Genetics and Genomics of the Triticeae, 2009

... Marco Maccaferri, Maria Corinna Sanguineti, Silvia Giuliani and Roberto Tuberosa ... C. Feuil... more ... Marco Maccaferri, Maria Corinna Sanguineti, Silvia Giuliani and Roberto Tuberosa ... C. Feuillet, GJ Muehlbauer (eds.), Genetics and Genomics of the Triticeae, Plant Genetics and Genomics: Crops and Models 7, DOI 10.1007/978-0-387-77489-3_18, ?? Springer Science ... Salem ...

ressources.ciheam.org

... C. Royo***, D. Villegas***, F. Alvaro***, M. Moragues***, JL ,Araus-Ortega***** M. Ben Salem*... more ... C. Royo***, D. Villegas***, F. Alvaro***, M. Moragues***, JL ,Araus-Ortega***** M. Ben Salem***** J. Bort***** E. DeAmbrogio**, A. Demontis**, A. El-Ahmed***** LF Garcia del Moral****, J. Isidro****, F. Maalouf***** M. Maccaferri*, V. Martos****, J. Motawai ...

Department of Plant …, 2000

Thrombosis Research, 1991

Using an ACL 300R coagulometer (Instrumentation Laboratory) we assessed the clinical usefulness o... more Using an ACL 300R coagulometer (Instrumentation Laboratory) we assessed the clinical usefulness of a new method to measure PS activity (PS:Act), based on the prolongation of prothrombin time of a mixture of diluted plasma sample, PS depleted plasma previously incubated with Protac for protein C activation, bovine thromboplastin and calcium ions. The results were compared with those from immunological assays. PS:Act was measured in 42 apparently healthy subjects, in 12 patients with hereditary PS deficiency (HPSD group) diagnosed on the basis of immunologic tests and in 48 patients with episodes of juvenile venous thromboembolism at least three months prior to testing (JVTE group). All the HPSD patients had PS:Act below the normal range (less than 62%). In JVTE group 9 patients (18.7%) showed abnormal results for PS:Act, 4 (8.3%) had low levels of free PS:Ag; all patients had normal total PS:Ag levels. Levels of antiphospholipid antibodies (immunologic test) were normal in the 9 JVTE patients with low PS:Act. When all the results were considered together (n = 102), the correlation coefficient between PS:Act and free PS:Ag was 0.78 (p less than 0.01).

TAG Theoretical and Applied Genetics, 2003

It has been argued that the level of genetic diversity in the modern durum wheat (Triticum turgid... more It has been argued that the level of genetic diversity in the modern durum wheat (Triticum turgidum L. var. durum) elite germplasm may have declined due to the high selection pressure applied in breeding programs. In this study, 58 accessions covering a wide spectrum of genetic diversity of the cultivated durum wheat gene pool were characterized with 70 microsatellite loci (or simple sequence repeats, SSRs). On average, SSRs detected 5.6 different allelic variants per locus, with a mean diversity index (DI) equal to 0.56, thus revealing a diversity content comparable to those previously observed with SSRs in other small-grain cereal gene pools. The mean genetic similarity value was equal to 0.44. A highly diagnostic SSR set has been identified. A high variation in allele size was detected among SSR loci, suggesting a different suitability of these loci for estimating genetic diversity. The B genome was characterized by an overall polymorphism significantly higher than that of the A genome. Genetic diversity is organised in well-distinct sub-groups identified by the corresponding foundationgenotypes. A large portion (92.7%) of the molecular variation detected within the group of 45 modern cvs was accounted for by SSR alleles tracing back to ten foundation-genotypes; among those, the most recent CIMMYT-derived founders were genetically distant from the old Mediterranean ones. On the other hand, rare alleles were abundant, suggesting that a large number of genetic introgressions contributed to the foundation of the well-diversified germplasm herein considered. The profiles of recently released varieties indicate that the level of genetic diversity present in the modern durum wheat germplasm has actually increased over time.

Theoretical and Applied Genetics, 2008

A recombinant inbred line (RIL) population and a set of advanced lines from multiple crosses were... more A recombinant inbred line (RIL) population and a set of advanced lines from multiple crosses were used to investigate the leaf rust (Puccinia triticina Eriks.) resistance carried by the durum wheat cultivar Creso and its derivatives (Colosseo and Plinio). One hundred seventysix RILs from the cross Colosseo 9 Lloyd were tested under artificial rust inoculation in the field. The response at the seedling stage was also investigated. A major QTL (QLr.ubo-7B.2) for leaf rust resistance controlling both the seedling and the adult open field based-response was mapped on 7BL, with the favourable allele inherited from Colosseo. QLr.ubo-7B.2 showed R 2 and LOD peak values for the area under disease progress curve (AUDPC) equal to 72.9% and 44.5, respectively. The presence and location of QLr.ubo-7B.2 was validated by a linkage disequilibrium-based test using two-year field data of 62 advanced lines from 21 crosses with Creso, Colosseo or Plinio as resistance donors. QLr.ubo-7B.2 maps in a gene-dense region (7BL10-0.78-1.00) carrying several genes/QTLs in wheat and barley for resistance to rusts and other fungal diseases.

Crop stress management and global climate change, 2011

... et al., 2010). However, it should be noted that other studies in rice have reported a weak or... more ... et al., 2010). However, it should be noted that other studies in rice have reported a weak or even absence of correlation between root features and drought resistance (Pantuwan et al., 2002; Subashri et al., 2009). The final effects ...

Annals of botany, 2002

Comparative analysis of a number of studies in drought-stressed maize (Zea mays L.) reporting qua... more Comparative analysis of a number of studies in drought-stressed maize (Zea mays L.) reporting quantitative trait loci (QTLs) for abscisic acid concentration, root characteristics, other morpho-physiological traits (MPTs) and grain yield (GY) reveals their complex genetic basis and the influence of the genetic background and the environment on QTL effects. Chromosome regions (e.g. near umc11 on chromosome 1 and near csu133 on chromosome 2) with QTLs controlling a number of MPTs and GY across populations and conditions of different water supply have been identified. Examples are presented on the use of QTL information to elucidate the genetic and physiological bases of the association among MPTs and GY. The QTL approach allows us to develop hypotheses accounting for these associations which can be further tested by developing near isogenic lines (NILs) differing for the QTL alleles. NILs also allow for a more accurate assessment of the breeding value of MPTs and, in some cases, may al...

Fibrinolysis, 1990

Summary The traditional assays for measuring fibrin/ogen degradation products (FDP) have limitati... more Summary The traditional assays for measuring fibrin/ogen degradation products (FDP) have limitations due to the use of serum, and do not discriminate between FDPs deriving from fibrin or fibrinogen. The crosslinked fibrin derivatives may be detected by means of ...

Fibrinolysis, 1988

Abstract Fibrinolysis and related parameters were studied before and after a venous occlusion tes... more Abstract Fibrinolysis and related parameters were studied before and after a venous occlusion test in a group of obese non diabetic children, in comparison with an age and sex-matched control group. Obese children (exceeding by at least 25% the upper limit of ...

Genetic analysis of SBCMV resistance in durum wheat was carried out using a population of 181 rec... more Genetic analysis of SBCMV resistance in durum wheat was carried out using a population of 181 recombinant inbred lines (RILs) obtained from Meridiano (moderately resistant) × Claudio (moderately susceptible). The RILs were characterized for SBCMV response in the field under severe and uniform SBCMV infection during 2007 and 2008 and profiled with SSR and DArT markers. A wide range of

BMC Genomics, 2014

Durum wheat (Triticum durum Desf.) is a tetraploid cereal grown in the medium to low-precipitatio... more Durum wheat (Triticum durum Desf.) is a tetraploid cereal grown in the medium to low-precipitation areas of the Mediterranean Basin, North America and South-West Asia. Genomics applications in durum wheat have the potential to boost exploitation of genetic resources and to advance understanding of the genetics of important complex traits (e.g. resilience to environmental and biotic stresses). A dense and accurate consensus map specific for T. durum will greatly facilitate genetic mapping, functional genomics and marker-assisted improvement. High quality genotypic data from six core recombinant inbred line populations were used to obtain a consensus framework map of 598 simple sequence repeats (SSR) and Diversity Array Technology® (DArT) anchor markers (common across populations). Interpolation of unique markers from 14 maps allowed us to position a total of 2,575 markers in a consensus map of 2,463 cM. The T. durum A and B genomes were covered in their near totality based on the reference SSR hexaploid wheat map. The consensus locus order compared to those of the single component maps showed good correspondence, (average Spearman's rank correlation rho ρ value of 0.96). Differences in marker order and local recombination rate were observed between the durum and hexaploid wheat consensus maps. The consensus map was used to carry out a whole-genome search for genetic differentiation signatures and association to heading date in a panel of 183 accessions adapted to the Mediterranean areas. Linkage disequilibrium was found to decay below the r2 threshold=0.3 within 2.20 cM, on average. Strong molecular differentiations among sub-populations were mapped to 87 chromosome regions. A genome-wide association scan for heading date from 27 field trials in the Mediterranean Basin and in Mexico yielded 50 chromosome regions with evidences of association in multiple environments. The consensus map presented here was used as a reference for genetic diversity and mapping analyses in T. durum, providing nearly complete genome coverage and even marker density. Markers previously mapped in hexaploid wheat constitute a strong link between the two species. The consensus map provides the basis for high-density single nucleotide polymorphic (SNP) marker implementation in durum wheat.

Fibrinolysis, 1990

Plasminogen activator inhibitor (PA&l) antigen and fibrinjogen degradation products (FbDP, FgDP) ... more Plasminogen activator inhibitor (PA&l) antigen and fibrinjogen degradation products (FbDP, FgDP) were measured before and 8 days after gynaecological surgery in 50 patients randomly submitted to prophylaxis with low molecular weight heparin (Alfa LMWl-OP 2123, Alfa Wassermann, Bologna, Italy; LMWH group) or standard calcium heparin (CH group). All patients were screened for deep venous thrombosis by the 1251 Fibrinogen uptake test. In the CH group, but not in LMWH group, postoperative PAI-levels were significantly higher (~~0.01) than at baseline. The percent variation of PAI-between pre-and postoperative values was significantly higher in the CH than in LMWH group (~~0.01). Postoperative FbDP values were significantly increased in both groups (~~0.01) but FgDP only in LMWH group (pcO.01). No significant differences in pre-and postoperative PAI-values were found between patients with positive or negative "'1 Fibrinogen uptake test.

Plant Biotechnology Journal, 2014

Genetics and Genomics of the Triticeae, 2009

... Marco Maccaferri, Maria Corinna Sanguineti, Silvia Giuliani and Roberto Tuberosa ... C. Feuil... more ... Marco Maccaferri, Maria Corinna Sanguineti, Silvia Giuliani and Roberto Tuberosa ... C. Feuillet, GJ Muehlbauer (eds.), Genetics and Genomics of the Triticeae, Plant Genetics and Genomics: Crops and Models 7, DOI 10.1007/978-0-387-77489-3_18, ?? Springer Science ... Salem ...

ressources.ciheam.org

... C. Royo***, D. Villegas***, F. Alvaro***, M. Moragues***, JL ,Araus-Ortega***** M. Ben Salem*... more ... C. Royo***, D. Villegas***, F. Alvaro***, M. Moragues***, JL ,Araus-Ortega***** M. Ben Salem***** J. Bort***** E. DeAmbrogio**, A. Demontis**, A. El-Ahmed***** LF Garcia del Moral****, J. Isidro****, F. Maalouf***** M. Maccaferri*, V. Martos****, J. Motawai ...

Department of Plant …, 2000

Thrombosis Research, 1991

Using an ACL 300R coagulometer (Instrumentation Laboratory) we assessed the clinical usefulness o... more Using an ACL 300R coagulometer (Instrumentation Laboratory) we assessed the clinical usefulness of a new method to measure PS activity (PS:Act), based on the prolongation of prothrombin time of a mixture of diluted plasma sample, PS depleted plasma previously incubated with Protac for protein C activation, bovine thromboplastin and calcium ions. The results were compared with those from immunological assays. PS:Act was measured in 42 apparently healthy subjects, in 12 patients with hereditary PS deficiency (HPSD group) diagnosed on the basis of immunologic tests and in 48 patients with episodes of juvenile venous thromboembolism at least three months prior to testing (JVTE group). All the HPSD patients had PS:Act below the normal range (less than 62%). In JVTE group 9 patients (18.7%) showed abnormal results for PS:Act, 4 (8.3%) had low levels of free PS:Ag; all patients had normal total PS:Ag levels. Levels of antiphospholipid antibodies (immunologic test) were normal in the 9 JVTE patients with low PS:Act. When all the results were considered together (n = 102), the correlation coefficient between PS:Act and free PS:Ag was 0.78 (p less than 0.01).

TAG Theoretical and Applied Genetics, 2003

It has been argued that the level of genetic diversity in the modern durum wheat (Triticum turgid... more It has been argued that the level of genetic diversity in the modern durum wheat (Triticum turgidum L. var. durum) elite germplasm may have declined due to the high selection pressure applied in breeding programs. In this study, 58 accessions covering a wide spectrum of genetic diversity of the cultivated durum wheat gene pool were characterized with 70 microsatellite loci (or simple sequence repeats, SSRs). On average, SSRs detected 5.6 different allelic variants per locus, with a mean diversity index (DI) equal to 0.56, thus revealing a diversity content comparable to those previously observed with SSRs in other small-grain cereal gene pools. The mean genetic similarity value was equal to 0.44. A highly diagnostic SSR set has been identified. A high variation in allele size was detected among SSR loci, suggesting a different suitability of these loci for estimating genetic diversity. The B genome was characterized by an overall polymorphism significantly higher than that of the A genome. Genetic diversity is organised in well-distinct sub-groups identified by the corresponding foundationgenotypes. A large portion (92.7%) of the molecular variation detected within the group of 45 modern cvs was accounted for by SSR alleles tracing back to ten foundation-genotypes; among those, the most recent CIMMYT-derived founders were genetically distant from the old Mediterranean ones. On the other hand, rare alleles were abundant, suggesting that a large number of genetic introgressions contributed to the foundation of the well-diversified germplasm herein considered. The profiles of recently released varieties indicate that the level of genetic diversity present in the modern durum wheat germplasm has actually increased over time.

Theoretical and Applied Genetics, 2008

A recombinant inbred line (RIL) population and a set of advanced lines from multiple crosses were... more A recombinant inbred line (RIL) population and a set of advanced lines from multiple crosses were used to investigate the leaf rust (Puccinia triticina Eriks.) resistance carried by the durum wheat cultivar Creso and its derivatives (Colosseo and Plinio). One hundred seventysix RILs from the cross Colosseo 9 Lloyd were tested under artificial rust inoculation in the field. The response at the seedling stage was also investigated. A major QTL (QLr.ubo-7B.2) for leaf rust resistance controlling both the seedling and the adult open field based-response was mapped on 7BL, with the favourable allele inherited from Colosseo. QLr.ubo-7B.2 showed R 2 and LOD peak values for the area under disease progress curve (AUDPC) equal to 72.9% and 44.5, respectively. The presence and location of QLr.ubo-7B.2 was validated by a linkage disequilibrium-based test using two-year field data of 62 advanced lines from 21 crosses with Creso, Colosseo or Plinio as resistance donors. QLr.ubo-7B.2 maps in a gene-dense region (7BL10-0.78-1.00) carrying several genes/QTLs in wheat and barley for resistance to rusts and other fungal diseases.